Lag0005836 (gene) Sponge gourd (AG‐4) v1

BLAST of Lag0005836 vs. NCBI nr
Match: XP_023538150.1 (U-box domain-containing protein 9-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 573.2 bits (1476), Expect = 2.0e-159
Identity = 305/457 (66.74%), Postives = 364/457 (79.65%), Query Frame = 0

Query: 1   MADDKPMDLLKKDLKNLLDRIVVEEDFDFSTADEAIEKLTALKISKIAAASP-------- 60
           MAD + ++LLKKD+K LL RIV ++DF+ S ADEAIEKL +LK++K+AAA+         
Sbjct: 1   MADKESLELLKKDIKELLQRIVDQDDFECSIADEAIEKLNSLKLTKMAAAAAAAAAAAAA 60

Query: 61  ----ESGESSGSKTLVLPEMFLCPISGEFMKDPVLLQTGQTYDRSFIEKWFHKGHNTCPK 120
               ESG  SG K LV+P+ F CPISGE M+DPVLL+TGQTYDR FI KWF+KGHNTCP+
Sbjct: 61  AAPVESGGDSGRKALVMPDEFRCPISGELMEDPVLLKTGQTYDRPFINKWFNKGHNTCPR 120

Query: 121 TKEVLSDMTLTPNLLLRSMMIQWYAENGLPSPDVILEEAIANADESRLDELLNKLLSRSL 180
           T EVL DMTLTPN  LRSM+++W  +N L  P +  EE +A+A ES LDELLN+LLS SL
Sbjct: 121 TNEVLHDMTLTPNRSLRSMILKWCIDNKLELPKLTYEETVAHATESHLDELLNQLLSSSL 180

Query: 181 SDQKEAAKELRRIARLNHEFRALFASLPGSIERLLHPLSVENVNLHPDLQEDLITTVLNI 240
           SDQK AAKELR+IA+ NHEFR+LFA LP SIERLLHP+SV++VNLHPDLQEDLITTVLNI
Sbjct: 181 SDQKLAAKELRQIAKWNHEFRSLFAKLPDSIERLLHPISVDHVNLHPDLQEDLITTVLNI 240

Query: 241 SIHDANKKYLAEEPLVLPLLIQSLQYGSTEVKANAAAAIFSLAFHEENKNLIGNAGALKP 300
           S+   NKK L E  LVL LLIQSLQYGS+E KANAAAAIFSL+ ++ENK  IGNAGA+K 
Sbjct: 241 SLSGDNKKRLVENQLVLSLLIQSLQYGSSEAKANAAAAIFSLSLYQENKIAIGNAGAIKH 300

Query: 301 LILLLDDGHPVVIRDAASAIFQLCTTLENKEKAVCSGAVGSIVRNISQGIMVDELVSVLA 360
           L+ LLD+GHPVV+RDAASAIF LCT   NKEKAV SG V S++RNI++G++VDELVSVLA
Sbjct: 301 LVGLLDEGHPVVMRDAASAIFTLCTASANKEKAVSSGVVVSVLRNITEGVLVDELVSVLA 360

Query: 361 LLSTNLKAIDEMCKLKTVPCLLRIMRKTTSQGVKENCASILFIICTADRSKWTEIQDDEN 420
           LLS N+KAI  MCKL  V C+LR++R++TSQGVKENC SIL  I +  RSK  EIQ+DEN
Sbjct: 361 LLSNNMKAISIMCKLNAVSCMLRVIRESTSQGVKENCISILDTISSTYRSKLKEIQEDEN 420

Query: 421 ANGTILDLSQTGTSRARRKALGILEKLRRAASITHTA 446
           ANG I  LS+ G SRA+RKA  +LEKL+RA SITHTA
Sbjct: 421 ANGMISKLSRNGNSRAKRKATSVLEKLQRAGSITHTA 457

BLAST of Lag0005836 vs. NCBI nr
Match: XP_022965721.1 (U-box domain-containing protein 9-like [Cucurbita maxima])

HSP 1 Score: 573.2 bits (1476), Expect = 2.0e-159
Identity = 304/456 (66.67%), Postives = 365/456 (80.04%), Query Frame = 0

Query: 1   MADDKPMDLLKKDLKNLLDRIVVEEDFDFSTADEAIEKLTALKISKIAAASP-------- 60
           MAD + ++LLKKD+K+LL RIV ++DF+ S ADEAIEKL +LK++K+AAA+         
Sbjct: 1   MADKESLELLKKDIKDLLQRIVDQDDFECSIADEAIEKLNSLKLTKMAAAAAAAAAAAAA 60

Query: 61  ---ESGESSGSKTLVLPEMFLCPISGEFMKDPVLLQTGQTYDRSFIEKWFHKGHNTCPKT 120
              ESG  SG   LV+P+ F CPISGE M+DPVLL+TGQTYDR FI KWF+KGHNTCP+T
Sbjct: 61  PPLESGGDSGRTALVMPDEFRCPISGELMEDPVLLKTGQTYDRPFINKWFNKGHNTCPRT 120

Query: 121 KEVLSDMTLTPNLLLRSMMIQWYAENGLPSPDVILEEAIANADESRLDELLNKLLSRSLS 180
            EVL DMTLTPN  LRSM+++W  +N L  P +  EE +A+A ES LDELLN+LLS SLS
Sbjct: 121 NEVLHDMTLTPNRSLRSMILKWCIDNKLELPKLTYEETVAHATESHLDELLNQLLSSSLS 180

Query: 181 DQKEAAKELRRIARLNHEFRALFASLPGSIERLLHPLSVENVNLHPDLQEDLITTVLNIS 240
           DQK AAKELR+IA+ NHEFR+LFA LP SIERLLHP+SV++VNLHPDLQEDLITTVLNIS
Sbjct: 181 DQKLAAKELRQIAKWNHEFRSLFAKLPDSIERLLHPISVDHVNLHPDLQEDLITTVLNIS 240

Query: 241 IHDANKKYLAEEPLVLPLLIQSLQYGSTEVKANAAAAIFSLAFHEENKNLIGNAGALKPL 300
           +   NKK L E  LVL LLIQSLQYGS+E KANAAAAIFSL+ ++ENK  IGNAGA+K L
Sbjct: 241 LSGDNKKRLVENQLVLSLLIQSLQYGSSEAKANAAAAIFSLSLYQENKIAIGNAGAIKHL 300

Query: 301 ILLLDDGHPVVIRDAASAIFQLCTTLENKEKAVCSGAVGSIVRNISQGIMVDELVSVLAL 360
           + LLD+GHPVV+RDAASAIF LCT   NKEKAV SG V S++RNI++G++VDELVSVLAL
Sbjct: 301 VGLLDEGHPVVMRDAASAIFSLCTASANKEKAVSSGVVVSVLRNITEGVLVDELVSVLAL 360

Query: 361 LSTNLKAIDEMCKLKTVPCLLRIMRKTTSQGVKENCASILFIICTADRSKWTEIQDDENA 420
           LS N+KAI  MCKL  V C+LR++R++TSQGVKENC SIL  I +  RSK  EIQ+DENA
Sbjct: 361 LSNNMKAISIMCKLNAVSCMLRVIRESTSQGVKENCISILDTISSTYRSKLKEIQEDENA 420

Query: 421 NGTILDLSQTGTSRARRKALGILEKLRRAASITHTA 446
           NG I  LS+ G SRA+RKA+ +LEKL+RA SITHTA
Sbjct: 421 NGMISKLSRNGNSRAKRKAMSVLEKLQRAGSITHTA 456

BLAST of Lag0005836 vs. NCBI nr
Match: KAG7021008.1 (U-box domain-containing protein 9, partial [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 570.9 bits (1470), Expect = 9.9e-159
Identity = 304/458 (66.38%), Postives = 363/458 (79.26%), Query Frame = 0

Query: 1   MADDKPMDLLKKDLKNLLDRIVVEEDFDFSTADEAIEKLTALKISKIAAASP-------- 60
           MAD + ++LLKKD+K LL RIV ++DF+ S ADEAIEKL +LK++K+AAA+         
Sbjct: 1   MADKESLELLKKDIKELLQRIVDQDDFECSIADEAIEKLNSLKLTKMAAAAAASAAAAAA 60

Query: 61  -----ESGESSGSKTLVLPEMFLCPISGEFMKDPVLLQTGQTYDRSFIEKWFHKGHNTCP 120
                ESG  S    LV+P+ F CPISGE M+DPVLL+TGQTYDR FI KWF+KGHNTCP
Sbjct: 61  AAAPVESGGDSARTALVMPDEFRCPISGELMEDPVLLKTGQTYDRPFINKWFNKGHNTCP 120

Query: 121 KTKEVLSDMTLTPNLLLRSMMIQWYAENGLPSPDVILEEAIANADESRLDELLNKLLSRS 180
           +T EVL DMTLTPN  LRSM+++W  +N L  P +  EE +A+A ES LDELLN+LLS S
Sbjct: 121 RTNEVLHDMTLTPNRSLRSMILKWCIDNKLELPKLTYEETVAHATESHLDELLNQLLSSS 180

Query: 181 LSDQKEAAKELRRIARLNHEFRALFASLPGSIERLLHPLSVENVNLHPDLQEDLITTVLN 240
           LSDQK AAKELR+IA+ NHEFR+LFA LP SIERLLHP+SV++VNLHPDLQEDLITTVLN
Sbjct: 181 LSDQKLAAKELRQIAKWNHEFRSLFAKLPDSIERLLHPISVDHVNLHPDLQEDLITTVLN 240

Query: 241 ISIHDANKKYLAEEPLVLPLLIQSLQYGSTEVKANAAAAIFSLAFHEENKNLIGNAGALK 300
           IS+   NKK L E  LVL LLIQSLQYGS+E KANAAAAIFSL+ ++ENK  IGNAGA+K
Sbjct: 241 ISLSGDNKKRLVENQLVLSLLIQSLQYGSSEAKANAAAAIFSLSLYQENKIAIGNAGAIK 300

Query: 301 PLILLLDDGHPVVIRDAASAIFQLCTTLENKEKAVCSGAVGSIVRNISQGIMVDELVSVL 360
            L+ LLD+GHPVV+RDAASAIF LCT   NKEKAV SG V S++RNI++G++VDELVSVL
Sbjct: 301 HLVGLLDEGHPVVMRDAASAIFTLCTASANKEKAVSSGVVVSVLRNITEGVLVDELVSVL 360

Query: 361 ALLSTNLKAIDEMCKLKTVPCLLRIMRKTTSQGVKENCASILFIICTADRSKWTEIQDDE 420
           ALLS N+KAI  MCKL  V C+LR++R++TSQGVKENC SIL  I +  RSK  EIQ+DE
Sbjct: 361 ALLSNNMKAISIMCKLNAVSCMLRVIRESTSQGVKENCISILDTISSTYRSKLKEIQEDE 420

Query: 421 NANGTILDLSQTGTSRARRKALGILEKLRRAASITHTA 446
           NANG I  LSQ G SRA+RKA+ +LEKL+RA SITHTA
Sbjct: 421 NANGMISKLSQNGNSRAKRKAMSVLEKLQRAGSITHTA 458

BLAST of Lag0005836 vs. NCBI nr
Match: XP_022937712.1 (U-box domain-containing protein 9-like [Cucurbita moschata])

HSP 1 Score: 570.5 bits (1469), Expect = 1.3e-158
Identity = 303/455 (66.59%), Postives = 363/455 (79.78%), Query Frame = 0

Query: 1   MADDKPMDLLKKDLKNLLDRIVVEEDFDFSTADEAIEKLTALKISKIAAASP-------- 60
           MAD + ++LLKKD+K LL RIV ++DF+ S ADEAIEKL +LK++K+AAAS         
Sbjct: 1   MADKESLELLKKDIKELLQRIVDQDDFECSIADEAIEKLNSLKLTKMAAASAAAAAAAAA 60

Query: 61  --ESGESSGSKTLVLPEMFLCPISGEFMKDPVLLQTGQTYDRSFIEKWFHKGHNTCPKTK 120
             ESG  S    LV+P+ F CPISGE M+DPVLL+TGQTYDR FI KWF+KGHNTCP+T 
Sbjct: 61  PVESGGDSARTALVMPDEFRCPISGELMEDPVLLKTGQTYDRPFINKWFNKGHNTCPRTN 120

Query: 121 EVLSDMTLTPNLLLRSMMIQWYAENGLPSPDVILEEAIANADESRLDELLNKLLSRSLSD 180
           EVL DMTLTPN  LRSM+++W  +N L  P +  EE +A+A ES LDELLN+LLS SLSD
Sbjct: 121 EVLHDMTLTPNRSLRSMILKWCIDNKLELPKLTYEETVAHATESHLDELLNQLLSSSLSD 180

Query: 181 QKEAAKELRRIARLNHEFRALFASLPGSIERLLHPLSVENVNLHPDLQEDLITTVLNISI 240
           QK AAKELR+IA+ NHEFR+LFA LP SIERLLHP+SV++VNLHPDLQEDLITTVLNIS+
Sbjct: 181 QKLAAKELRQIAKWNHEFRSLFAKLPDSIERLLHPISVDHVNLHPDLQEDLITTVLNISL 240

Query: 241 HDANKKYLAEEPLVLPLLIQSLQYGSTEVKANAAAAIFSLAFHEENKNLIGNAGALKPLI 300
              NKK L E  LVL LLIQSLQYGS+E KANAAAAIFSL+ ++ENK  IGNAGA+K L+
Sbjct: 241 SGDNKKRLVENQLVLSLLIQSLQYGSSEAKANAAAAIFSLSLYQENKIAIGNAGAIKHLV 300

Query: 301 LLLDDGHPVVIRDAASAIFQLCTTLENKEKAVCSGAVGSIVRNISQGIMVDELVSVLALL 360
            LLD+GHPVV+RDAASAIF LCT   NKEKAV SG V S++RNI++G++VDELVSVLALL
Sbjct: 301 GLLDEGHPVVMRDAASAIFTLCTASANKEKAVSSGVVVSVLRNITEGVLVDELVSVLALL 360

Query: 361 STNLKAIDEMCKLKTVPCLLRIMRKTTSQGVKENCASILFIICTADRSKWTEIQDDENAN 420
           S N+KAI  MCKL  V C+L+++R++TSQGVKENC SIL  I +  RSK  EIQ+DENAN
Sbjct: 361 SNNMKAISIMCKLNAVSCMLKVIRESTSQGVKENCISILDTISSTYRSKLKEIQEDENAN 420

Query: 421 GTILDLSQTGTSRARRKALGILEKLRRAASITHTA 446
           G I  LS+ G SRA+RKA+ +LEKL+RA SITHTA
Sbjct: 421 GMISKLSRNGNSRAKRKAMSVLEKLQRAGSITHTA 455

BLAST of Lag0005836 vs. NCBI nr
Match: KAG6586176.1 (U-box domain-containing protein 9, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 567.8 bits (1462), Expect = 8.4e-158
Identity = 302/459 (65.80%), Postives = 363/459 (79.08%), Query Frame = 0

Query: 1   MADDKPMDLLKKDLKNLLDRIVVEEDFDFSTADEAIEKLTALKISKIAAASP-------- 60
           MAD + ++LLKKD+K LL RIV ++DF+ S ADEAIEKL +LK++K+AAA+         
Sbjct: 1   MADKESLELLKKDIKELLQRIVDQDDFECSIADEAIEKLNSLKLTKMAAAAAASAAAAAA 60

Query: 61  ------ESGESSGSKTLVLPEMFLCPISGEFMKDPVLLQTGQTYDRSFIEKWFHKGHNTC 120
                 ESG  S    LV+P+ F CPISGE M+DPVLL+TGQTYDR FI KWF+KGHNTC
Sbjct: 61  AAAAPVESGGDSARTALVMPDEFRCPISGELMEDPVLLKTGQTYDRPFINKWFNKGHNTC 120

Query: 121 PKTKEVLSDMTLTPNLLLRSMMIQWYAENGLPSPDVILEEAIANADESRLDELLNKLLSR 180
           P+T EVL DMTLTPN  LRSM+++W  +N L  P +  EE +A+A ES LDELLN+LLS 
Sbjct: 121 PRTNEVLHDMTLTPNRSLRSMILKWCIDNKLELPKLTYEETVAHATESHLDELLNQLLSS 180

Query: 181 SLSDQKEAAKELRRIARLNHEFRALFASLPGSIERLLHPLSVENVNLHPDLQEDLITTVL 240
           SLSDQK AAKELR+IA+ NHEFR+LFA LP SIERLLHP+SV++VNLHPDLQEDLITTVL
Sbjct: 181 SLSDQKLAAKELRQIAKWNHEFRSLFAKLPDSIERLLHPISVDHVNLHPDLQEDLITTVL 240

Query: 241 NISIHDANKKYLAEEPLVLPLLIQSLQYGSTEVKANAAAAIFSLAFHEENKNLIGNAGAL 300
           NIS+   NKK L E  LVL LLIQSLQYGS+E KANAAAAIFSL+ ++ENK  IGNAGA+
Sbjct: 241 NISLSGDNKKRLVENQLVLSLLIQSLQYGSSEAKANAAAAIFSLSLYQENKIAIGNAGAI 300

Query: 301 KPLILLLDDGHPVVIRDAASAIFQLCTTLENKEKAVCSGAVGSIVRNISQGIMVDELVSV 360
           K L+ LLD+GHPVV+RDAASAIF LCT   NKEKAV SG V S++RNI++G++VDELVSV
Sbjct: 301 KHLVGLLDEGHPVVMRDAASAIFTLCTASANKEKAVSSGVVVSVLRNITEGVLVDELVSV 360

Query: 361 LALLSTNLKAIDEMCKLKTVPCLLRIMRKTTSQGVKENCASILFIICTADRSKWTEIQDD 420
           LALLS N+KAI  MCKL  V C+L+++R++TSQGVKENC SIL  I +  RSK  EIQ+D
Sbjct: 361 LALLSNNMKAISIMCKLNAVSCMLKVIRESTSQGVKENCISILDTISSTYRSKLKEIQED 420

Query: 421 ENANGTILDLSQTGTSRARRKALGILEKLRRAASITHTA 446
           ENANG I  LS+ G SRA+RKA+ +LEKL+RA SITHTA
Sbjct: 421 ENANGMISKLSRNGNSRAKRKAMSVLEKLQRAGSITHTA 459

BLAST of Lag0005836 vs. ExPASy Swiss-Prot
Match: Q9SRT0 (U-box domain-containing protein 9 OS=Arabidopsis thaliana OX=3702 GN=PUB9 PE=1 SV=1)

HSP 1 Score: 374.8 bits (961), Expect = 1.4e-102
Identity = 210/443 (47.40%), Postives = 303/443 (68.40%), Query Frame = 0

Query: 10  LKKDLKNLLDRIVVEEDFDFSTADEAIEKLTALKISKIAAASPESGESSGSKTLVLPEMF 69
           LK+++K LL +I  E+D    T D+  + L+AL+ + +   + +S      +T+  PE F
Sbjct: 20  LKREMKKLLIKIDDEDDLGVQTIDQLQDALSALREATMRKMA-KSSSLEMLETVSCPEEF 79

Query: 70  LCPISGEFMKDPVLLQTGQTYDRSFIEKWFHKGHNTCPKTKEVLSDMTLTPNLLLRSMMI 129
            CP+S E M+DPV+L +GQTYD+ FI+KW   G+ TCPKT++VL    LTPNLL+R M+ 
Sbjct: 80  RCPLSNELMRDPVVLASGQTYDKLFIQKWLSSGNRTCPKTQQVLPHTALTPNLLIREMIS 139

Query: 130 QWYAENGLPS-----PDVILE-EAIANADESRLDELLNKLLSRSLSDQKEAAKELRRIAR 189
           +W  +NGL +     P+++ E E +  +D    + LL K+ S +L DQK AAKELR + R
Sbjct: 140 KWCKKNGLETKSQYHPNLVNEDETVTRSDREIFNSLLCKVSSSNLQDQKSAAKELRLLTR 199

Query: 190 LNHEFRALFASLPGSIERLLHPLSVENVNLHPDLQEDLITTVLNISIH-DANKKYLAEEP 249
              EFRALF   P  I RL++PL +   N    LQED++TT+LNISIH D+NKK + E P
Sbjct: 200 KGTEFRALFGESPDEITRLVNPL-LHGSNPDEKLQEDVVTTLLNISIHDDSNKKLVCENP 259

Query: 250 LVLPLLIQSLQYGSTEVKANAAAAIFSLAFHEENKNLIGNAGALKPLILLLDDGHPVVIR 309
            V+PLLI +L+ G+   ++NAAAAIF+L+  + NK LIG +G LKPLI LL++G+P+ I+
Sbjct: 260 NVIPLLIDALRRGTVATRSNAAAAIFTLSALDSNKVLIGKSGILKPLIDLLEEGNPLAIK 319

Query: 310 DAASAIFQLCTTLENKEKAVCSGAVGSIVRNISQGIMVDELVSVLALLSTNLKAIDEMCK 369
           D A+AIF LC   EN+ +AV  GAV  + + IS G+ VDEL+++LA+L T+ KA++E+ +
Sbjct: 320 DVAAAIFTLCIAHENRSRAVRDGAVRVLGKKISNGLYVDELLAILAMLVTHWKAVEELGE 379

Query: 370 LKTVPCLLRIMRKTTSQGVKENCASILFIICTADRSKWTEIQDDENANGTILDLSQTGTS 429
           L  V  LL+I R++  +  KEN   IL  IC +DR+KW EI+++ENA+GTI  LS+ GTS
Sbjct: 380 LGGVSWLLKITRESECKRNKENAIVILHTICFSDRTKWKEIKEEENAHGTITKLSREGTS 439

Query: 430 RARRKALGILEKLRRAASITHTA 446
           RA+RKA GIL++LR+A ++THTA
Sbjct: 440 RAQRKANGILDRLRKAMNLTHTA 460

BLAST of Lag0005836 vs. ExPASy Swiss-Prot
Match: Q3E9F7 (Putative U-box domain-containing protein 46 OS=Arabidopsis thaliana OX=3702 GN=PUB46 PE=3 SV=1)

HSP 1 Score: 251.1 bits (640), Expect = 2.3e-65
Identity = 170/447 (38.03%), Postives = 264/447 (59.06%), Query Frame = 0

Query: 8   DLLKKDLKNLLDRIVVE---EDFDFSTA--------DEAIEKLTALKISKIAAASPESGE 67
           D L+++L+ +L  I+ +    D D + A        DEA+  LT L+  K+ +  PES  
Sbjct: 10  DTLRRELQKVLTEILNDGGGNDRDETEAFSGVVKAIDEAVRILTCLR--KVESKIPESDI 69

Query: 68  SSGSKTLVLPEMFLCPISGEFMKDPVLLQTGQTYDRSFIEKWFHKGHNTCPKTKEVLSDM 127
           S     + +P+ F+C +S   M +PV++ +GQTY++ +I +W  K   TCPKTK+VLS  
Sbjct: 70  S----PVEVPKEFICTLSNTIMIEPVIIASGQTYEKRYITEWL-KHERTCPKTKQVLSHR 129

Query: 128 TLTPNLLLRSMMIQWYAENGLPSPDVILEEAIANADESRLDELLNKL-LSRSLSDQKEAA 187
              PN L+  ++ QW   N         +E +A    S ++ LL ++  S S++DQ EAA
Sbjct: 130 LWIPNHLISDLITQWCLVNKYDHQKP-SDELVAELFTSDIEALLQRVSSSSSVADQIEAA 189

Query: 188 KELRRIARLNHEFRALF-ASLPGSIERLLHPLSV--ENVNLHPDLQEDLITTVLNISIHD 247
           KELR   +     R  F A +  SI RLL PLS   E V+   +LQE+++T + N+SI +
Sbjct: 190 KELRHQTKKFPNVRVFFVAGIHDSITRLLSPLSTLDEAVDSSLELQENIVTALFNLSILE 249

Query: 248 ANKKYLAEEPLVLPLLIQSLQYGSTEVKANAAAAIFSLAFHEENKNLIGNAGALKPLILL 307
           +NK  +AE  LV+PLL +SL+ G+ E + NAAA + SL+  + NK +IGN+ A+K LI L
Sbjct: 250 SNKTVIAENCLVIPLLTKSLKQGTDETRRNAAATLSSLSAIDSNKIIIGNSEAVKALIDL 309

Query: 308 LDDGHPVVIRDAASAIFQLCTTLENKEKAVCSGAVGSIVRNISQGIMVDELVSVLALLST 367
           +++G  +  ++A S +F LC  LENK K V +G + +  + I  G  VDEL+S+LAL+ST
Sbjct: 310 IEEGDLLATKEATSTVFNLCIVLENKGKVVSAGLIHAATKKIKAGSNVDELLSLLALIST 369

Query: 368 NLKAIDEMCKLKTVPCLLRIMRKTTSQGVKENCASILFIIC--TADRSKWTEIQDDENAN 427
           + +A++EM KL  +  L  I+RK +S    EN   I+F +     DRS+   + ++EN +
Sbjct: 370 HNRAVEEMDKLGFIYDLFSILRKPSSLLTGENAVVIVFNMYDRNRDRSRLKVVGEEENQH 429

Query: 428 GTILDLSQTGTSRARRKALGILEKLRR 438
           GT   L++ G+ RA RKA GIL+ ++R
Sbjct: 430 GTFTKLAKQGSVRAARKAQGILQWIKR 448

BLAST of Lag0005836 vs. ExPASy Swiss-Prot
Match: Q8GUG9 (U-box domain-containing protein 11 OS=Arabidopsis thaliana OX=3702 GN=PUB11 PE=2 SV=2)

HSP 1 Score: 245.4 bits (625), Expect = 1.3e-63
Identity = 151/387 (39.02%), Postives = 240/387 (62.02%), Query Frame = 0

Query: 56  ESSGSKTLVLPEMFLCPISGEFMKDPVLLQTGQTYDRSFIEKWFHKGHNTCPKTKEVLSD 115
           ES  S  L +P  FLCP+S E MKDPV++ TGQTY+R++I++W   G+ TCPKT++ L +
Sbjct: 232 ESKKSDKLTIPVDFLCPVSLELMKDPVIVATGQTYERAYIQRWIDCGNLTCPKTQQKLEN 291

Query: 116 MTLTPNLLLRSMMIQWYAENGLPSPDVILEEAIANA-DESRLDELLNKLLSRSLSDQKEA 175
            TLTPN +LRS++ +W AE+ +  P   +     N+ D S +  L+ +L SRS  D++ A
Sbjct: 292 FTLTPNYVLRSLISRWCAEHNIEQPAGYINGRTKNSGDMSVIRALVQRLSSRSTEDRRNA 351

Query: 176 AKELRRIARLNHEFRALFASLPGSIERLLHPLSVENVNLHPDLQEDLITTVLNISIHDAN 235
             E+R +++ + + R L A   G+I  L++ L+ E+V      QE+ IT VLN+SI++ N
Sbjct: 352 VSEIRSLSKRSTDNRILIAE-AGAIPVLVNLLTSEDV----ATQENAITCVLNLSIYENN 411

Query: 236 KKYLAEEPLVLPLLIQSLQYGSTEVKANAAAAIFSLAFHEENKNLIGNAGALKPLILLLD 295
           K+ +     V   ++Q L+ G+ E + NAAA +FSL+  +ENK +IG +GA+  L+ LL+
Sbjct: 412 KELIMFAGAVTS-IVQVLRAGTMEARENAAATLFSLSLADENKIIIGGSGAIPALVDLLE 471

Query: 296 DGHPVVIRDAASAIFQLCTTLENKEKAVCSGAVGSIVRNISQGI---MVDELVSVLALLS 355
           +G P   +DAA+A+F LC    NK +AV +G V ++V+ +S      MVDE +++L++L+
Sbjct: 472 NGTPRGKKDAATALFNLCIYHGNKGRAVRAGIVTALVKMLSDSTRHRMVDEALTILSVLA 531

Query: 356 TNLKAIDEMCKLKTVPCLLRIMRKTTSQGVKENCASILFIICTADRSKWTEIQDDENANG 415
            N  A   + K  T+P L+ I++   ++  +EN A+IL  +C  D  K   I     A  
Sbjct: 532 NNQDAKSAIVKANTLPALIGILQTDQTRN-RENAAAILLSLCKRDTEKLITI-GRLGAVV 591

Query: 416 TILDLSQTGTSRARRKALGILEKLRRA 439
            ++DLS+ GT R +RKA+ +LE LR+A
Sbjct: 592 PLMDLSKNGTERGKRKAISLLELLRKA 610

BLAST of Lag0005836 vs. ExPASy Swiss-Prot
Match: Q5VRH9 (U-box domain-containing protein 12 OS=Oryza sativa subsp. japonica OX=39947 GN=PUB12 PE=2 SV=1)

HSP 1 Score: 238.0 bits (606), Expect = 2.0e-61
Identity = 165/443 (37.25%), Postives = 252/443 (56.88%), Query Frame = 0

Query: 4   DKPMDLLKKDLKNLLDRIVVEEDFDFSTADEAIEKLTALKISKIAAASPESGESSGSKTL 63
           D  +D +   LK L D +V E        D A + LT  + + I   SP           
Sbjct: 187 DGCVDQMSSLLKKLKDCVVTE--------DHANDALTT-RSASIKHRSP----------- 246

Query: 64  VLPEMFLCPISGEFMKDPVLLQTGQTYDRSFIEKWFHKGHNTCPKTKEVLSDMTLTPNLL 123
           ++P+ F CPIS E M+DPV++ +GQTY+RS I+KW   GH TCPKT++ LS  +LTPN +
Sbjct: 247 IIPDEFRCPISLELMQDPVIVSSGQTYERSCIQKWLDSGHKTCPKTQQPLSHTSLTPNFV 306

Query: 124 LRSMMIQWYAENGLPSPDVILEEAIANA------DESRLDELLNKLLSRSLSDQKEAAKE 183
           L+S++ QW   NG+  P          A      D + L  L+N+L S +  +Q+ AA E
Sbjct: 307 LKSLISQWCEANGIELPKNKQNSRDKKAAKSSDYDHAGLVSLMNRLRSGNQDEQRAAAGE 366

Query: 184 LRRIARLNHEFRALFASLPGSIERLLHPLSVENVNLHPDLQEDLITTVLNISIHDANKKY 243
           +R +A+ N   R   A   G+I  L++ LS  +    P  QE  +T +LN+SIH+ NK  
Sbjct: 367 IRLLAKRNVNNRICIAE-AGAIPLLVNLLSSSD----PRTQEHAVTALLNLSIHENNKAS 426

Query: 244 LAEEPLVLPLLIQSLQYGSTEVKANAAAAIFSLAFHEENKNLIGNAGALKPLILLLDDGH 303
           + +    +P +++ L+ GS E + NAAA +FSL+  +ENK  IG AGA+ PLI LL DG 
Sbjct: 427 IVDSH-AIPKIVEVLKTGSMETRENAAATLFSLSVVDENKVTIGAAGAIPPLINLLCDGS 486

Query: 304 PVVIRDAASAIFQLCTTLENKEKAVCSGAVGSIVRNI--SQGIMVDELVSVLALLSTNLK 363
           P   +DAA+AIF LC    NK +AV +G V  ++  +    G M+DE +S+L++L+ N +
Sbjct: 487 PRGKKDAATAIFNLCIYQGNKVRAVKAGIVIHLMNFLVDPTGGMIDEALSLLSILAGNPE 546

Query: 364 AIDEMCKLKTVPCLLRIMRKTTSQGVKENCASILFIICTADRSKWTEIQDDENANGTILD 423
               + + + +P L+ ++ KT S   +EN A+IL+++C+AD ++ T           + +
Sbjct: 547 GKIVIARSEPIPPLVEVI-KTGSPRNRENAAAILWLLCSAD-TEQTLAAKAAGVEDALKE 601

Query: 424 LSQTGTSRARRKALGILEKLRRA 439
           LS+TGT RA+RKA  ILE + +A
Sbjct: 607 LSETGTDRAKRKASSILELMHQA 601

BLAST of Lag0005836 vs. ExPASy Swiss-Prot
Match: Q8VZ40 (U-box domain-containing protein 14 OS=Arabidopsis thaliana OX=3702 GN=PUB14 PE=1 SV=1)

HSP 1 Score: 233.4 bits (594), Expect = 4.9e-60
Identity = 159/454 (35.02%), Postives = 253/454 (55.73%), Query Frame = 0

Query: 7   MDLLKKDLKNLLDRIVVEEDFDFSTADEAIEKLTALKISKIAAASPESGESSGSKTL--- 66
           +D LKK+  + +    +  D D     E +  L    +  +   S +   S+GS+ +   
Sbjct: 185 IDELKKE-SHAIHEYFLSYDGDPDDCFERMSSLLKNLVDFVTMESSDPDPSTGSRIVSRH 244

Query: 67  ---VLPEMFLCPISGEFMKDPVLLQTGQTYDRSFIEKWFHKGHNTCPKTKEVLSDMTLTP 126
              V+PE F CPIS E MKDPV++ TGQTY+RS I+KW   GH TCPK++E L    LTP
Sbjct: 245 RSPVIPEYFRCPISLELMKDPVIVSTGQTYERSSIQKWLDAGHKTCPKSQETLLHAGLTP 304

Query: 127 NLLLRSMMIQWYAENGLPSPD--------VILEEAIANADESRLDELLNKLLSRSLSDQK 186
           N +L+S++  W   NG+  P          I   + ++ D + +  LL KL + +   Q+
Sbjct: 305 NYVLKSLIALWCESNGIELPQNQGSCRTTKIGGSSSSDCDRTFVLSLLEKLANGTTEQQR 364

Query: 187 EAAKELRRIARLNHEFRALFASLPGSIERLLHPLSVENVNLHPDLQEDLITTVLNISIHD 246
            AA ELR +A+ N + R   A   G+I  L+  LS  +    P  QE  +T +LN+SI++
Sbjct: 365 AAAGELRLLAKRNVDNRVCIAE-AGAIPLLVELLSSPD----PRTQEHSVTALLNLSINE 424

Query: 247 ANKKYLAEEPLVLPLLIQSLQYGSTEVKANAAAAIFSLAFHEENKNLIGNAGALKPLILL 306
            NK  + +   +   +++ L+ GS E + NAAA +FSL+  +ENK  IG AGA++ LI L
Sbjct: 425 GNKGAIVDAGAITD-IVEVLKNGSMEARENAAATLFSLSVIDENKVAIGAAGAIQALISL 484

Query: 307 LDDGHPVVIRDAASAIFQLCTTLENKEKAVCSGAVGSIVRNISQ--GIMVDELVSVLALL 366
           L++G     +DAA+AIF LC    NK +AV  G V  + R +    G MVDE +++LA+L
Sbjct: 485 LEEGTRRGKKDAATAIFNLCIYQGNKSRAVKGGIVDPLTRLLKDAGGGMVDEALAILAIL 544

Query: 367 STNLKAIDEMCKLKTVPCLLRIMRKTTSQGVKENCASILFIICTADRSKWTEIQDDENAN 426
           STN +    + + +++P L+ I+R T S   +EN A+IL+ +C  +  +   +  +  A+
Sbjct: 545 STNQEGKTAIAEAESIPVLVEIIR-TGSPRNRENAAAILWYLCIGNIER-LNVAREVGAD 604

Query: 427 GTILDLSQTGTSRARRKALGILEKLRRAASITHT 445
             + +L++ GT RA+RKA  +LE +++   +  T
Sbjct: 605 VALKELTENGTDRAKRKAASLLELIQQTEGVAVT 629

BLAST of Lag0005836 vs. ExPASy TrEMBL
Match: A0A6J1HMF9 (RING-type E3 ubiquitin transferase OS=Cucurbita maxima OX=3661 GN=LOC111465532 PE=4 SV=1)

HSP 1 Score: 573.2 bits (1476), Expect = 9.7e-160
Identity = 304/456 (66.67%), Postives = 365/456 (80.04%), Query Frame = 0

Query: 1   MADDKPMDLLKKDLKNLLDRIVVEEDFDFSTADEAIEKLTALKISKIAAASP-------- 60
           MAD + ++LLKKD+K+LL RIV ++DF+ S ADEAIEKL +LK++K+AAA+         
Sbjct: 1   MADKESLELLKKDIKDLLQRIVDQDDFECSIADEAIEKLNSLKLTKMAAAAAAAAAAAAA 60

Query: 61  ---ESGESSGSKTLVLPEMFLCPISGEFMKDPVLLQTGQTYDRSFIEKWFHKGHNTCPKT 120
              ESG  SG   LV+P+ F CPISGE M+DPVLL+TGQTYDR FI KWF+KGHNTCP+T
Sbjct: 61  PPLESGGDSGRTALVMPDEFRCPISGELMEDPVLLKTGQTYDRPFINKWFNKGHNTCPRT 120

Query: 121 KEVLSDMTLTPNLLLRSMMIQWYAENGLPSPDVILEEAIANADESRLDELLNKLLSRSLS 180
            EVL DMTLTPN  LRSM+++W  +N L  P +  EE +A+A ES LDELLN+LLS SLS
Sbjct: 121 NEVLHDMTLTPNRSLRSMILKWCIDNKLELPKLTYEETVAHATESHLDELLNQLLSSSLS 180

Query: 181 DQKEAAKELRRIARLNHEFRALFASLPGSIERLLHPLSVENVNLHPDLQEDLITTVLNIS 240
           DQK AAKELR+IA+ NHEFR+LFA LP SIERLLHP+SV++VNLHPDLQEDLITTVLNIS
Sbjct: 181 DQKLAAKELRQIAKWNHEFRSLFAKLPDSIERLLHPISVDHVNLHPDLQEDLITTVLNIS 240

Query: 241 IHDANKKYLAEEPLVLPLLIQSLQYGSTEVKANAAAAIFSLAFHEENKNLIGNAGALKPL 300
           +   NKK L E  LVL LLIQSLQYGS+E KANAAAAIFSL+ ++ENK  IGNAGA+K L
Sbjct: 241 LSGDNKKRLVENQLVLSLLIQSLQYGSSEAKANAAAAIFSLSLYQENKIAIGNAGAIKHL 300

Query: 301 ILLLDDGHPVVIRDAASAIFQLCTTLENKEKAVCSGAVGSIVRNISQGIMVDELVSVLAL 360
           + LLD+GHPVV+RDAASAIF LCT   NKEKAV SG V S++RNI++G++VDELVSVLAL
Sbjct: 301 VGLLDEGHPVVMRDAASAIFSLCTASANKEKAVSSGVVVSVLRNITEGVLVDELVSVLAL 360

Query: 361 LSTNLKAIDEMCKLKTVPCLLRIMRKTTSQGVKENCASILFIICTADRSKWTEIQDDENA 420
           LS N+KAI  MCKL  V C+LR++R++TSQGVKENC SIL  I +  RSK  EIQ+DENA
Sbjct: 361 LSNNMKAISIMCKLNAVSCMLRVIRESTSQGVKENCISILDTISSTYRSKLKEIQEDENA 420

Query: 421 NGTILDLSQTGTSRARRKALGILEKLRRAASITHTA 446
           NG I  LS+ G SRA+RKA+ +LEKL+RA SITHTA
Sbjct: 421 NGMISKLSRNGNSRAKRKAMSVLEKLQRAGSITHTA 456

BLAST of Lag0005836 vs. ExPASy TrEMBL
Match: A0A6J1FC00 (RING-type E3 ubiquitin transferase OS=Cucurbita moschata OX=3662 GN=LOC111444033 PE=4 SV=1)

HSP 1 Score: 570.5 bits (1469), Expect = 6.3e-159
Identity = 303/455 (66.59%), Postives = 363/455 (79.78%), Query Frame = 0

Query: 1   MADDKPMDLLKKDLKNLLDRIVVEEDFDFSTADEAIEKLTALKISKIAAASP-------- 60
           MAD + ++LLKKD+K LL RIV ++DF+ S ADEAIEKL +LK++K+AAAS         
Sbjct: 1   MADKESLELLKKDIKELLQRIVDQDDFECSIADEAIEKLNSLKLTKMAAASAAAAAAAAA 60

Query: 61  --ESGESSGSKTLVLPEMFLCPISGEFMKDPVLLQTGQTYDRSFIEKWFHKGHNTCPKTK 120
             ESG  S    LV+P+ F CPISGE M+DPVLL+TGQTYDR FI KWF+KGHNTCP+T 
Sbjct: 61  PVESGGDSARTALVMPDEFRCPISGELMEDPVLLKTGQTYDRPFINKWFNKGHNTCPRTN 120

Query: 121 EVLSDMTLTPNLLLRSMMIQWYAENGLPSPDVILEEAIANADESRLDELLNKLLSRSLSD 180
           EVL DMTLTPN  LRSM+++W  +N L  P +  EE +A+A ES LDELLN+LLS SLSD
Sbjct: 121 EVLHDMTLTPNRSLRSMILKWCIDNKLELPKLTYEETVAHATESHLDELLNQLLSSSLSD 180

Query: 181 QKEAAKELRRIARLNHEFRALFASLPGSIERLLHPLSVENVNLHPDLQEDLITTVLNISI 240
           QK AAKELR+IA+ NHEFR+LFA LP SIERLLHP+SV++VNLHPDLQEDLITTVLNIS+
Sbjct: 181 QKLAAKELRQIAKWNHEFRSLFAKLPDSIERLLHPISVDHVNLHPDLQEDLITTVLNISL 240

Query: 241 HDANKKYLAEEPLVLPLLIQSLQYGSTEVKANAAAAIFSLAFHEENKNLIGNAGALKPLI 300
              NKK L E  LVL LLIQSLQYGS+E KANAAAAIFSL+ ++ENK  IGNAGA+K L+
Sbjct: 241 SGDNKKRLVENQLVLSLLIQSLQYGSSEAKANAAAAIFSLSLYQENKIAIGNAGAIKHLV 300

Query: 301 LLLDDGHPVVIRDAASAIFQLCTTLENKEKAVCSGAVGSIVRNISQGIMVDELVSVLALL 360
            LLD+GHPVV+RDAASAIF LCT   NKEKAV SG V S++RNI++G++VDELVSVLALL
Sbjct: 301 GLLDEGHPVVMRDAASAIFTLCTASANKEKAVSSGVVVSVLRNITEGVLVDELVSVLALL 360

Query: 361 STNLKAIDEMCKLKTVPCLLRIMRKTTSQGVKENCASILFIICTADRSKWTEIQDDENAN 420
           S N+KAI  MCKL  V C+L+++R++TSQGVKENC SIL  I +  RSK  EIQ+DENAN
Sbjct: 361 SNNMKAISIMCKLNAVSCMLKVIRESTSQGVKENCISILDTISSTYRSKLKEIQEDENAN 420

Query: 421 GTILDLSQTGTSRARRKALGILEKLRRAASITHTA 446
           G I  LS+ G SRA+RKA+ +LEKL+RA SITHTA
Sbjct: 421 GMISKLSRNGNSRAKRKAMSVLEKLQRAGSITHTA 455

BLAST of Lag0005836 vs. ExPASy TrEMBL
Match: A0A6J1CT87 (RING-type E3 ubiquitin transferase OS=Momordica charantia OX=3673 GN=LOC111014117 PE=4 SV=1)

HSP 1 Score: 557.8 bits (1436), Expect = 4.2e-155
Identity = 299/452 (66.15%), Postives = 367/452 (81.19%), Query Frame = 0

Query: 1   MADDK-PMDLLKKDLKNLLDRIVVEEDFDFSTADEAIEKLTALKISKIAAASPESGESSG 60
           MA++K P + +KKDL+ LL++I  +ED++  +ADEAI++L ALK+S+I  AS  +  SS 
Sbjct: 1   MAEEKPPSEPIKKDLEKLLNKITHQEDWEIVSADEAIDRLDALKLSRI--ASTAACGSSR 60

Query: 61  SKTLVLPEMFLCPISGEFMKDPVLLQTGQTYDRSFIEKWFHKGHNTCPKTKEVLSDMTLT 120
           S++  +PE F CPISGE MKDPV+L+TGQTYDR+FIEKWFHKGHNTCP+TKEVLSDMTLT
Sbjct: 61  SRSPFVPEFFRCPISGELMKDPVILKTGQTYDRAFIEKWFHKGHNTCPQTKEVLSDMTLT 120

Query: 121 PNLLLRSMMIQWYAENGLPSPDVILEEAIANADESRLDELLNKLL-----SRSLSDQKEA 180
           PN LLR ++ QW AENGL  P ++ EE + +  E   DELLNKL      S SLSDQK A
Sbjct: 121 PNRLLREIISQWCAENGLELPKLVFEEVVTSESECDFDELLNKLSLSLSPSSSLSDQKAA 180

Query: 181 AKELRRIARLNHEFRALFASLPGSIERLLHPLSVENVNLHPDLQEDLITTVLNISIHDAN 240
           AKELRR+A+ NHEFRALFA  P SI+RLL  L ++N++LHPDLQEDL+T+VLNISIH+ N
Sbjct: 181 AKELRRLAKGNHEFRALFAKRPESIDRLLSCLLLDNISLHPDLQEDLVTSVLNISIHEDN 240

Query: 241 KKYLAEEPLVLPLLIQSLQYGSTEVKANAAAAIFSLAFHEENKNLIGNAGALKPLILLL- 300
           KK++AE  L LP+LI+SLQYG+T  KANAAAAI SLA  E NK +IGNAGALKPLI LL 
Sbjct: 241 KKHVAENWLALPVLIESLQYGNTNTKANAAAAISSLACLESNKIIIGNAGALKPLIALLD 300

Query: 301 DDGHPVVIRDAASAIFQLCTTLENKEKAVCSGAVGSIVRNISQGIMVDELVSVLALLSTN 360
           DD HPVVI+DAASAIF+LCT  +NKE+AV +GAVGS++RNI  GIMVDELVSVLALLS+N
Sbjct: 301 DDAHPVVIKDAASAIFRLCTIHQNKEQAVYNGAVGSVLRNIIGGIMVDELVSVLALLSSN 360

Query: 361 LKAIDEMCKLKTVPCLLRIMRKTTSQGVKENCASILFIICTADRSKWTEIQDDENANGTI 420
            KA++EMCKL TVPC+L IMR+ +++ VKENC SILF ICT++R K  EIQ+DEN NGTI
Sbjct: 361 RKAVEEMCKLDTVPCMLEIMREASAECVKENCVSILFAICTSNRRKMWEIQEDENWNGTI 420

Query: 421 LDLSQTGTSRARRKALGILEKLRRAASITHTA 446
           ++LS +G SRARRKA+GILE+L RAAS THTA
Sbjct: 421 VELSTSGNSRARRKAIGILERLHRAASRTHTA 450

BLAST of Lag0005836 vs. ExPASy TrEMBL
Match: A0A5A7UZN6 (RING-type E3 ubiquitin transferase OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold165G00400 PE=4 SV=1)

HSP 1 Score: 538.1 bits (1385), Expect = 3.5e-149
Identity = 285/442 (64.48%), Postives = 348/442 (78.73%), Query Frame = 0

Query: 7   MDLLKKDLKNLLDRIVVEEDFDFSTADEAIEKLTALKISKI-AAASPESGESSGSKTLVL 66
           MDLLKKDLK +L RI+ EEDFD S AD+AI+ LT+LK S + A +S ES   SGSKTL++
Sbjct: 3   MDLLKKDLKQVLYRIIQEEDFDSSAADDAIQILTSLKFSLMPAPSSQESPGCSGSKTLII 62

Query: 67  PEMFLCPISGEFMKDPVLLQTGQTYDRSFIEKWFHKGHNTCPKTKEVLSDMTLTPNLLLR 126
           P+ F CPISG+ MKDPVLL TGQTYDR FIEKWFH+GHNTCP+TKEVL+DMTLTPN LLR
Sbjct: 63  PDKFRCPISGDLMKDPVLLITGQTYDRFFIEKWFHEGHNTCPQTKEVLTDMTLTPNRLLR 122

Query: 127 SMMIQWYAENGLPSPDVILEEAIANADESRLDELLNKLLSRSLSDQKEAAKELRRIARLN 186
           SM+ QW  +N    P +  EE + N  ES LD LLN+L+S SL  +KEAAKELR   R +
Sbjct: 123 SMISQWCLDNRFELPRLSYEEEVDNVTESHLDALLNRLMSSSLIVKKEAAKELRETTRCS 182

Query: 187 HEFRALFASLPGSIERLLHPL-SVENVNLHPDLQEDLITTVLNISIHDANKKYLAEEPLV 246
           HEFRALFA LPGS+ERLLHPL S+  V+LHPDLQEDLITT+LNIS+ D NK+++ E PLV
Sbjct: 183 HEFRALFAKLPGSVERLLHPLISIGKVDLHPDLQEDLITTILNISVFDDNKEHVVENPLV 242

Query: 247 LPLLIQSLQYGSTEVKANAAAAIFSLAFHEENKNLIGNAGALKPLILLLDDGHPVVIRDA 306
           LPLLI+SLQ+GS E+ ANA AAI+SL+F E NK  +G  G  K LI LLD  HP V RDA
Sbjct: 243 LPLLIESLQHGSIELTANAVAAIYSLSFSEANKITMGKVGIFKHLISLLDYAHPGVTRDA 302

Query: 307 ASAIFQLCTTLENKEKAVCSGAVGSIVRNISQG-IMVDELVSVLALLSTNLKAIDEMCKL 366
            SAI+ LCTT+EN+EKAV SG V +I RNI++  ++VD+L+S+LALL T++KAIDEMCK 
Sbjct: 303 GSAIYNLCTTVENREKAVGSGVVAAIFRNIARSLLLVDKLISILALLCTDVKAIDEMCKF 362

Query: 367 KTVPCLLRIMRKTTSQGVKENCASILFIICTADRSKWTEIQDDENANGTILDLSQTGTSR 426
             VPC+LR +R+T SQ +KENCASILF ICT D+S+  +IQ+DEN N TIL+LS+ G SR
Sbjct: 363 DVVPCMLRFIRETESQRIKENCASILFAICTTDQSQLRKIQEDENKNETILELSKNGNSR 422

Query: 427 ARRKALGILEKLRRAASITHTA 446
           A RKA GILE+++RAAS THT+
Sbjct: 423 ASRKATGILERMQRAASRTHTS 444

BLAST of Lag0005836 vs. ExPASy TrEMBL
Match: A0A1S3B4X7 (RING-type E3 ubiquitin transferase OS=Cucumis melo OX=3656 GN=LOC103485858 PE=4 SV=1)

HSP 1 Score: 538.1 bits (1385), Expect = 3.5e-149
Identity = 285/442 (64.48%), Postives = 348/442 (78.73%), Query Frame = 0

Query: 7   MDLLKKDLKNLLDRIVVEEDFDFSTADEAIEKLTALKISKI-AAASPESGESSGSKTLVL 66
           MDLLKKDLK +L RI+ EEDFD S AD+AI+ LT+LK S + A +S ES   SGSKTL++
Sbjct: 3   MDLLKKDLKQVLYRIIQEEDFDSSAADDAIQILTSLKFSLMPAPSSQESPGCSGSKTLII 62

Query: 67  PEMFLCPISGEFMKDPVLLQTGQTYDRSFIEKWFHKGHNTCPKTKEVLSDMTLTPNLLLR 126
           P+ F CPISG+ MKDPVLL TGQTYDR FIEKWFH+GHNTCP+TKEVL+DMTLTPN LLR
Sbjct: 63  PDKFRCPISGDLMKDPVLLITGQTYDRFFIEKWFHEGHNTCPQTKEVLTDMTLTPNRLLR 122

Query: 127 SMMIQWYAENGLPSPDVILEEAIANADESRLDELLNKLLSRSLSDQKEAAKELRRIARLN 186
           SM+ QW  +N    P +  EE + N  ES LD LLN+L+S SL  +KEAAKELR   R +
Sbjct: 123 SMISQWCLDNRFELPRLSYEEEVDNVTESHLDALLNRLMSSSLIVKKEAAKELRETTRCS 182

Query: 187 HEFRALFASLPGSIERLLHPL-SVENVNLHPDLQEDLITTVLNISIHDANKKYLAEEPLV 246
           HEFRALFA LPGS+ERLLHPL S+  V+LHPDLQEDLITT+LNIS+ D NK+++ E PLV
Sbjct: 183 HEFRALFAKLPGSVERLLHPLISIGKVDLHPDLQEDLITTILNISVFDDNKEHVVENPLV 242

Query: 247 LPLLIQSLQYGSTEVKANAAAAIFSLAFHEENKNLIGNAGALKPLILLLDDGHPVVIRDA 306
           LPLLI+SLQ+GS E+ ANA AAI+SL+F E NK  +G  G  K LI LLD  HP V RDA
Sbjct: 243 LPLLIESLQHGSIELTANAVAAIYSLSFSEANKITMGKVGIFKHLISLLDYAHPGVTRDA 302

Query: 307 ASAIFQLCTTLENKEKAVCSGAVGSIVRNISQG-IMVDELVSVLALLSTNLKAIDEMCKL 366
            SAI+ LCTT+EN+EKAV SG V +I RNI++  ++VD+L+S+LALL T++KAIDEMCK 
Sbjct: 303 GSAIYNLCTTVENREKAVGSGVVAAIFRNIARSLLLVDKLISILALLCTDVKAIDEMCKF 362

Query: 367 KTVPCLLRIMRKTTSQGVKENCASILFIICTADRSKWTEIQDDENANGTILDLSQTGTSR 426
             VPC+LR +R+T SQ +KENCASILF ICT D+S+  +IQ+DEN N TIL+LS+ G SR
Sbjct: 363 DVVPCMLRFIRETESQRIKENCASILFAICTTDQSQLRKIQEDENKNETILELSKNGNSR 422

Query: 427 ARRKALGILEKLRRAASITHTA 446
           A RKA GILE+++RAAS THT+
Sbjct: 423 ASRKATGILERMQRAASRTHTS 444

BLAST of Lag0005836 vs. TAIR 10
Match: AT3G07360.1 (plant U-box 9 )

HSP 1 Score: 374.8 bits (961), Expect = 9.7e-104
Identity = 210/443 (47.40%), Postives = 303/443 (68.40%), Query Frame = 0

Query: 10  LKKDLKNLLDRIVVEEDFDFSTADEAIEKLTALKISKIAAASPESGESSGSKTLVLPEMF 69
           LK+++K LL +I  E+D    T D+  + L+AL+ + +   + +S      +T+  PE F
Sbjct: 20  LKREMKKLLIKIDDEDDLGVQTIDQLQDALSALREATMRKMA-KSSSLEMLETVSCPEEF 79

Query: 70  LCPISGEFMKDPVLLQTGQTYDRSFIEKWFHKGHNTCPKTKEVLSDMTLTPNLLLRSMMI 129
            CP+S E M+DPV+L +GQTYD+ FI+KW   G+ TCPKT++VL    LTPNLL+R M+ 
Sbjct: 80  RCPLSNELMRDPVVLASGQTYDKLFIQKWLSSGNRTCPKTQQVLPHTALTPNLLIREMIS 139

Query: 130 QWYAENGLPS-----PDVILE-EAIANADESRLDELLNKLLSRSLSDQKEAAKELRRIAR 189
           +W  +NGL +     P+++ E E +  +D    + LL K+ S +L DQK AAKELR + R
Sbjct: 140 KWCKKNGLETKSQYHPNLVNEDETVTRSDREIFNSLLCKVSSSNLQDQKSAAKELRLLTR 199

Query: 190 LNHEFRALFASLPGSIERLLHPLSVENVNLHPDLQEDLITTVLNISIH-DANKKYLAEEP 249
              EFRALF   P  I RL++PL +   N    LQED++TT+LNISIH D+NKK + E P
Sbjct: 200 KGTEFRALFGESPDEITRLVNPL-LHGSNPDEKLQEDVVTTLLNISIHDDSNKKLVCENP 259

Query: 250 LVLPLLIQSLQYGSTEVKANAAAAIFSLAFHEENKNLIGNAGALKPLILLLDDGHPVVIR 309
            V+PLLI +L+ G+   ++NAAAAIF+L+  + NK LIG +G LKPLI LL++G+P+ I+
Sbjct: 260 NVIPLLIDALRRGTVATRSNAAAAIFTLSALDSNKVLIGKSGILKPLIDLLEEGNPLAIK 319

Query: 310 DAASAIFQLCTTLENKEKAVCSGAVGSIVRNISQGIMVDELVSVLALLSTNLKAIDEMCK 369
           D A+AIF LC   EN+ +AV  GAV  + + IS G+ VDEL+++LA+L T+ KA++E+ +
Sbjct: 320 DVAAAIFTLCIAHENRSRAVRDGAVRVLGKKISNGLYVDELLAILAMLVTHWKAVEELGE 379

Query: 370 LKTVPCLLRIMRKTTSQGVKENCASILFIICTADRSKWTEIQDDENANGTILDLSQTGTS 429
           L  V  LL+I R++  +  KEN   IL  IC +DR+KW EI+++ENA+GTI  LS+ GTS
Sbjct: 380 LGGVSWLLKITRESECKRNKENAIVILHTICFSDRTKWKEIKEEENAHGTITKLSREGTS 439

Query: 430 RARRKALGILEKLRRAASITHTA 446
           RA+RKA GIL++LR+A ++THTA
Sbjct: 440 RAQRKANGILDRLRKAMNLTHTA 460

BLAST of Lag0005836 vs. TAIR 10
Match: AT3G07360.2 (plant U-box 9 )

HSP 1 Score: 277.3 bits (708), Expect = 2.1e-74
Identity = 159/326 (48.77%), Postives = 227/326 (69.63%), Query Frame = 0

Query: 127 MMIQWYAENGLPS-----PDVILE-EAIANADESRLDELLNKLLSRSLSDQKEAAKELRR 186
           M+ +W  +NGL +     P+++ E E +  +D    + LL K+ S +L DQK AAKELR 
Sbjct: 1   MISKWCKKNGLETKSQYHPNLVNEDETVTRSDREIFNSLLCKVSSSNLQDQKSAAKELRL 60

Query: 187 IARLNHEFRALFASLPGSIERLLHPLSVENVNLHPDLQEDLITTVLNISIH-DANKKYLA 246
           + R   EFRALF   P  I RL++PL +   N    LQED++TT+LNISIH D+NKK + 
Sbjct: 61  LTRKGTEFRALFGESPDEITRLVNPL-LHGSNPDEKLQEDVVTTLLNISIHDDSNKKLVC 120

Query: 247 EEPLVLPLLIQSLQYGSTEVKANAAAAIFSLAFHEENKNLIGNAGALKPLILLLDDGHPV 306
           E P V+PLLI +L+ G+   ++NAAAAIF+L+  + NK LIG +G LKPLI LL++G+P+
Sbjct: 121 ENPNVIPLLIDALRRGTVATRSNAAAAIFTLSALDSNKVLIGKSGILKPLIDLLEEGNPL 180

Query: 307 VIRDAASAIFQLCTTLENKEKAVCSGAVGSIVRNISQGIMVDELVSVLALLSTNLKAIDE 366
            I+D A+AIF LC   EN+ +AV  GAV  + + IS G+ VDEL+++LA+L T+ KA++E
Sbjct: 181 AIKDVAAAIFTLCIAHENRSRAVRDGAVRVLGKKISNGLYVDELLAILAMLVTHWKAVEE 240

Query: 367 MCKLKTVPCLLRIMRKTTSQGVKENCASILFIICTADRSKWTEIQDDENANGTILDLSQT 426
           + +L  V  LL+I R++  +  KEN   IL  IC +DR+KW EI+++ENA+GTI  LS+ 
Sbjct: 241 LGELGGVSWLLKITRESECKRNKENAIVILHTICFSDRTKWKEIKEEENAHGTITKLSRE 300

Query: 427 GTSRARRKALGILEKLRRAASITHTA 446
           GTSRA+RKA GIL++LR+A ++THTA
Sbjct: 301 GTSRAQRKANGILDRLRKAMNLTHTA 325

BLAST of Lag0005836 vs. TAIR 10
Match: AT3G07360.3 (plant U-box 9 )

HSP 1 Score: 277.3 bits (708), Expect = 2.1e-74
Identity = 159/326 (48.77%), Postives = 227/326 (69.63%), Query Frame = 0

Query: 127 MMIQWYAENGLPS-----PDVILE-EAIANADESRLDELLNKLLSRSLSDQKEAAKELRR 186
           M+ +W  +NGL +     P+++ E E +  +D    + LL K+ S +L DQK AAKELR 
Sbjct: 1   MISKWCKKNGLETKSQYHPNLVNEDETVTRSDREIFNSLLCKVSSSNLQDQKSAAKELRL 60

Query: 187 IARLNHEFRALFASLPGSIERLLHPLSVENVNLHPDLQEDLITTVLNISIH-DANKKYLA 246
           + R   EFRALF   P  I RL++PL +   N    LQED++TT+LNISIH D+NKK + 
Sbjct: 61  LTRKGTEFRALFGESPDEITRLVNPL-LHGSNPDEKLQEDVVTTLLNISIHDDSNKKLVC 120

Query: 247 EEPLVLPLLIQSLQYGSTEVKANAAAAIFSLAFHEENKNLIGNAGALKPLILLLDDGHPV 306
           E P V+PLLI +L+ G+   ++NAAAAIF+L+  + NK LIG +G LKPLI LL++G+P+
Sbjct: 121 ENPNVIPLLIDALRRGTVATRSNAAAAIFTLSALDSNKVLIGKSGILKPLIDLLEEGNPL 180

Query: 307 VIRDAASAIFQLCTTLENKEKAVCSGAVGSIVRNISQGIMVDELVSVLALLSTNLKAIDE 366
            I+D A+AIF LC   EN+ +AV  GAV  + + IS G+ VDEL+++LA+L T+ KA++E
Sbjct: 181 AIKDVAAAIFTLCIAHENRSRAVRDGAVRVLGKKISNGLYVDELLAILAMLVTHWKAVEE 240

Query: 367 MCKLKTVPCLLRIMRKTTSQGVKENCASILFIICTADRSKWTEIQDDENANGTILDLSQT 426
           + +L  V  LL+I R++  +  KEN   IL  IC +DR+KW EI+++ENA+GTI  LS+ 
Sbjct: 241 LGELGGVSWLLKITRESECKRNKENAIVILHTICFSDRTKWKEIKEEENAHGTITKLSRE 300

Query: 427 GTSRARRKALGILEKLRRAASITHTA 446
           GTSRA+RKA GIL++LR+A ++THTA
Sbjct: 301 GTSRAQRKANGILDRLRKAMNLTHTA 325

BLAST of Lag0005836 vs. TAIR 10
Match: AT5G18320.1 (ARM repeat superfamily protein )

HSP 1 Score: 251.1 bits (640), Expect = 1.6e-66
Identity = 170/447 (38.03%), Postives = 264/447 (59.06%), Query Frame = 0

Query: 8   DLLKKDLKNLLDRIVVE---EDFDFSTA--------DEAIEKLTALKISKIAAASPESGE 67
           D L+++L+ +L  I+ +    D D + A        DEA+  LT L+  K+ +  PES  
Sbjct: 10  DTLRRELQKVLTEILNDGGGNDRDETEAFSGVVKAIDEAVRILTCLR--KVESKIPESDI 69

Query: 68  SSGSKTLVLPEMFLCPISGEFMKDPVLLQTGQTYDRSFIEKWFHKGHNTCPKTKEVLSDM 127
           S     + +P+ F+C +S   M +PV++ +GQTY++ +I +W  K   TCPKTK+VLS  
Sbjct: 70  S----PVEVPKEFICTLSNTIMIEPVIIASGQTYEKRYITEWL-KHERTCPKTKQVLSHR 129

Query: 128 TLTPNLLLRSMMIQWYAENGLPSPDVILEEAIANADESRLDELLNKL-LSRSLSDQKEAA 187
              PN L+  ++ QW   N         +E +A    S ++ LL ++  S S++DQ EAA
Sbjct: 130 LWIPNHLISDLITQWCLVNKYDHQKP-SDELVAELFTSDIEALLQRVSSSSSVADQIEAA 189

Query: 188 KELRRIARLNHEFRALF-ASLPGSIERLLHPLSV--ENVNLHPDLQEDLITTVLNISIHD 247
           KELR   +     R  F A +  SI RLL PLS   E V+   +LQE+++T + N+SI +
Sbjct: 190 KELRHQTKKFPNVRVFFVAGIHDSITRLLSPLSTLDEAVDSSLELQENIVTALFNLSILE 249

Query: 248 ANKKYLAEEPLVLPLLIQSLQYGSTEVKANAAAAIFSLAFHEENKNLIGNAGALKPLILL 307
           +NK  +AE  LV+PLL +SL+ G+ E + NAAA + SL+  + NK +IGN+ A+K LI L
Sbjct: 250 SNKTVIAENCLVIPLLTKSLKQGTDETRRNAAATLSSLSAIDSNKIIIGNSEAVKALIDL 309

Query: 308 LDDGHPVVIRDAASAIFQLCTTLENKEKAVCSGAVGSIVRNISQGIMVDELVSVLALLST 367
           +++G  +  ++A S +F LC  LENK K V +G + +  + I  G  VDEL+S+LAL+ST
Sbjct: 310 IEEGDLLATKEATSTVFNLCIVLENKGKVVSAGLIHAATKKIKAGSNVDELLSLLALIST 369

Query: 368 NLKAIDEMCKLKTVPCLLRIMRKTTSQGVKENCASILFIIC--TADRSKWTEIQDDENAN 427
           + +A++EM KL  +  L  I+RK +S    EN   I+F +     DRS+   + ++EN +
Sbjct: 370 HNRAVEEMDKLGFIYDLFSILRKPSSLLTGENAVVIVFNMYDRNRDRSRLKVVGEEENQH 429

Query: 428 GTILDLSQTGTSRARRKALGILEKLRR 438
           GT   L++ G+ RA RKA GIL+ ++R
Sbjct: 430 GTFTKLAKQGSVRAARKAQGILQWIKR 448

BLAST of Lag0005836 vs. TAIR 10
Match: AT1G23030.1 (ARM repeat superfamily protein )

HSP 1 Score: 245.4 bits (625), Expect = 8.9e-65
Identity = 151/387 (39.02%), Postives = 240/387 (62.02%), Query Frame = 0

Query: 56  ESSGSKTLVLPEMFLCPISGEFMKDPVLLQTGQTYDRSFIEKWFHKGHNTCPKTKEVLSD 115
           ES  S  L +P  FLCP+S E MKDPV++ TGQTY+R++I++W   G+ TCPKT++ L +
Sbjct: 232 ESKKSDKLTIPVDFLCPVSLELMKDPVIVATGQTYERAYIQRWIDCGNLTCPKTQQKLEN 291

Query: 116 MTLTPNLLLRSMMIQWYAENGLPSPDVILEEAIANA-DESRLDELLNKLLSRSLSDQKEA 175
            TLTPN +LRS++ +W AE+ +  P   +     N+ D S +  L+ +L SRS  D++ A
Sbjct: 292 FTLTPNYVLRSLISRWCAEHNIEQPAGYINGRTKNSGDMSVIRALVQRLSSRSTEDRRNA 351

Query: 176 AKELRRIARLNHEFRALFASLPGSIERLLHPLSVENVNLHPDLQEDLITTVLNISIHDAN 235
             E+R +++ + + R L A   G+I  L++ L+ E+V      QE+ IT VLN+SI++ N
Sbjct: 352 VSEIRSLSKRSTDNRILIAE-AGAIPVLVNLLTSEDV----ATQENAITCVLNLSIYENN 411

Query: 236 KKYLAEEPLVLPLLIQSLQYGSTEVKANAAAAIFSLAFHEENKNLIGNAGALKPLILLLD 295
           K+ +     V   ++Q L+ G+ E + NAAA +FSL+  +ENK +IG +GA+  L+ LL+
Sbjct: 412 KELIMFAGAVTS-IVQVLRAGTMEARENAAATLFSLSLADENKIIIGGSGAIPALVDLLE 471

Query: 296 DGHPVVIRDAASAIFQLCTTLENKEKAVCSGAVGSIVRNISQGI---MVDELVSVLALLS 355
           +G P   +DAA+A+F LC    NK +AV +G V ++V+ +S      MVDE +++L++L+
Sbjct: 472 NGTPRGKKDAATALFNLCIYHGNKGRAVRAGIVTALVKMLSDSTRHRMVDEALTILSVLA 531

Query: 356 TNLKAIDEMCKLKTVPCLLRIMRKTTSQGVKENCASILFIICTADRSKWTEIQDDENANG 415
            N  A   + K  T+P L+ I++   ++  +EN A+IL  +C  D  K   I     A  
Sbjct: 532 NNQDAKSAIVKANTLPALIGILQTDQTRN-RENAAAILLSLCKRDTEKLITI-GRLGAVV 591

Query: 416 TILDLSQTGTSRARRKALGILEKLRRA 439
            ++DLS+ GT R +RKA+ +LE LR+A
Sbjct: 592 PLMDLSKNGTERGKRKAISLLELLRKA 610