Lag0005732 (gene) Sponge gourd (AG‐4) v1

Overview
NameLag0005732
Typegene
OrganismLuffa acutangula (Sponge gourd (AG‐4) v1)
DescriptionGDSL esterase/lipase
Locationchr6: 27387250 .. 27389566 (+)
RNA-Seq ExpressionLag0005732
SyntenyLag0005732
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonCDSpolypeptide
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGAAGGCTCAATTGGTGGTGTATTGTTTTCTAACGATTGCTGCTTTTGGAACTCGTGGGGTTGATGGAAAATCAATAGTGCCTTGCTATTTTGTATTTGGGGACTCTCAAGCTGATAATGGAAATAACAACATCCTGAATATTGGGATGGCGAGGGCTGATTATAAGCCTTATGGCATTGACTTCTCAAAGCAACAGACTCCCACAGGGAGGTTCACCAATGGCCGAAACGTTCCTGATTTCATCGGTTTGCCTTCTCATTTCAATCTGGACTAAAATGGAACAAATTTGAAAATTTATCGTGGTCCTGTAATTTGATTGAATTAACGATTTTATTTACGTAATCTCAAATTTTCTTTTTCCAAATTAACACGGTTCAAAACCATATTGAAAATATATGTATTATATCATTTTATTATCCCTCTCTCTTATATTACTTTTCCATTCTAAAGATACATAAGATTGATAATGATGACCCTTTTCATTTTTCAGCTAAATTTTTGGGTTTTGTCGGTTACATCCCACCATTTGAGAATACAAAGGGTTCGGATATTCTCAAAGGTGTAAACTATGCATCCGGAGGTTCAGGAATTCTCCCGGAAACTGGAAGGCTACTGGTAACTTTTTTTTTTGAGAAGAACTGGTAACTAATTTAGAATTAGACTTGATCACTACACAGAATTGAATAGGAAATTTTTTGTTAATGAATTTCTCGTTCTATTAGTTTCATCTTTATTAAATTGAAACATATGTGGAGCGGAGATTTGAATCTCCCACCTTGAAAAAATGAGAAGATAGCTTAACCGCTGAGCTACATACATATATTAGTTGGTAGTTCATTCTTGAGGGAACTAAAAAAAAGGTTATTAGACTACATATAGAAGTTGAGAGCAAATAATCCATATAAAACCATTTGATTTAGTTCCTACGCTTCGCATAAAAAATCATTTAGTCCTTATTCATTAATTTTATAGGTTTATGTAGAGATAAGTATATATGAAGTTGGTCAACATGAGTATGACGTAAAATTTGTACCAATGGTCAAAAGGTCTATGGTTCATTTCCCCCACCCAGATGTTGTCAAACAAAAAAGTTACTTTCCTTTGTAAAAAAAGAAGAAGAAGAAGAATGTTAGGTGTTGAATTTTAAAACAGTTATGAATTTCTATTACATGTTTAATGGAGAATTAATGTTAAGCACTAAAATTAGTAATAATTTACACTAAATTTAGATACTAAATAGAACAAAAAAAAAAAAAAGGTAGAAAGAGAAAAATAGAACATTGTTTAATATAATCAAATGTATTGTGATCCTATATATGAGAACAATCAATTTTCTCTTTATGCTTGTAGGGCCAAATATCAAGCCTCGGCAAGCAATTGGAAAATCACAATCACACAATTTCAAAAATCCAAGCCTTATTAGGAAGGAAAAGTCGAACTACAGAATATCTTAAACGCTGCTTATATACAGTTCAAATTGGAAGCAATGATTACCTTAATAATTATTTTATGCCACTTAACTACAAAACAAGTTTCCAATTTACTCCCGAACATTTTGCCACCACCCTGAATAAAGAGCTCACTCGCCAATTGAAGGTCAGTTCCTACAAATGTTTCCTACGTACCACCATTAAAATATTGACAACTAAGAGAATTTTTTTATCTTTCCTTTAGTTGTCAACATTTGGATGGTGGTATGCAGTCAATTACGGTTATTTTATTTTTATTGAGGTTAAATTAATATGTCTCGAGATTGGATGGTAGACTTTATATGAGCAAGGAGCTAGAAAGGTAGCCGTTTTTGGAGTGGGATCCATAGGGTGTACTCCATATGCAAGGGCTAACTTTGAGCACAAAGAAACTCCATGTGTGGACAAAGTCAACAGTGCCGTTCAATTTTTCAATATTGGCCTCAAGTCCCTTGTTGAGGATCTTAATACCCAATTGCCCGATGCTAAGTTTATCTTCGTTGATGTCTTCCAAATTTCTACTCTTCAGCCTTCAAATATTGGTAACATTTCATGCAAATTTCTAATATTAATTTCGATTGAATTATGATTAGAACTTAGAAAAAGGATACAAATGAATTTGCAGGTAAAATGGTTCTAGATGAGCCTTGTTGTGAAGTTCTACCGGGGCAGTTGCAGTGTAGTCCTTTTGGAAAAGTATGTGGCGACAGGACCCAATACCTGTTTTGGGATGGAGTTCATCCAACTGAAATTGGATTTACGACAGTGGCAAGTAGAGCCTTTAATTCTCAACAACCTGGTGATACTCATCCCTTTGATATCAAACACCTTCTTCGCACCTCCTGA

mRNA sequence

ATGAAGGCTCAATTGGTGGTGTATTGTTTTCTAACGATTGCTGCTTTTGGAACTCGTGGGGTTGATGGAAAATCAATAGTGCCTTGCTATTTTGTATTTGGGGACTCTCAAGCTGATAATGGAAATAACAACATCCTGAATATTGGGATGGCGAGGGCTGATTATAAGCCTTATGGCATTGACTTCTCAAAGCAACAGACTCCCACAGGGAGGTTCACCAATGGCCGAAACGTTCCTGATTTCATCGCTAAATTTTTGGGTTTTGTCGGTTACATCCCACCATTTGAGAATACAAAGGGTTCGGATATTCTCAAAGGTGTAAACTATGCATCCGGAGGTTCAGGAATTCTCCCGGAAACTGGAAGGCTACTGGGCCAAATATCAAGCCTCGGCAAGCAATTGGAAAATCACAATCACACAATTTCAAAAATCCAAGCCTTATTAGGAAGGAAAAGTCGAACTACAGAATATCTTAAACGCTGCTTATATACAGTTCAAATTGGAAGCAATGATTACCTTAATAATTATTTTATGCCACTTAACTACAAAACAAGTTTCCAATTTACTCCCGAACATTTTGCCACCACCCTGAATAAAGAGCTCACTCGCCAATTGAAGACTTTATATGAGCAAGGAGCTAGAAAGGTAGCCGTTTTTGGAGTGGGATCCATAGGGTGTACTCCATATGCAAGGGCTAACTTTGAGCACAAAGAAACTCCATGTGTGGACAAAGTCAACAGTGCCGTTCAATTTTTCAATATTGGCCTCAAGTCCCTTGTTGAGGATCTTAATACCCAATTGCCCGATGCTAAGTTTATCTTCGTTGATGTCTTCCAAATTTCTACTCTTCAGCCTTCAAATATTGGTAAAATGGTTCTAGATGAGCCTTGTTGTGAAGTTCTACCGGGGCAGTTGCAGTGTAGTCCTTTTGGAAAAGTATGTGGCGACAGGACCCAATACCTGTTTTGGGATGGAGTTCATCCAACTGAAATTGGATTTACGACAGTGGCAAGTAGAGCCTTTAATTCTCAACAACCTGGTGATACTCATCCCTTTGATATCAAACACCTTCTTCGCACCTCCTGA

Coding sequence (CDS)

ATGAAGGCTCAATTGGTGGTGTATTGTTTTCTAACGATTGCTGCTTTTGGAACTCGTGGGGTTGATGGAAAATCAATAGTGCCTTGCTATTTTGTATTTGGGGACTCTCAAGCTGATAATGGAAATAACAACATCCTGAATATTGGGATGGCGAGGGCTGATTATAAGCCTTATGGCATTGACTTCTCAAAGCAACAGACTCCCACAGGGAGGTTCACCAATGGCCGAAACGTTCCTGATTTCATCGCTAAATTTTTGGGTTTTGTCGGTTACATCCCACCATTTGAGAATACAAAGGGTTCGGATATTCTCAAAGGTGTAAACTATGCATCCGGAGGTTCAGGAATTCTCCCGGAAACTGGAAGGCTACTGGGCCAAATATCAAGCCTCGGCAAGCAATTGGAAAATCACAATCACACAATTTCAAAAATCCAAGCCTTATTAGGAAGGAAAAGTCGAACTACAGAATATCTTAAACGCTGCTTATATACAGTTCAAATTGGAAGCAATGATTACCTTAATAATTATTTTATGCCACTTAACTACAAAACAAGTTTCCAATTTACTCCCGAACATTTTGCCACCACCCTGAATAAAGAGCTCACTCGCCAATTGAAGACTTTATATGAGCAAGGAGCTAGAAAGGTAGCCGTTTTTGGAGTGGGATCCATAGGGTGTACTCCATATGCAAGGGCTAACTTTGAGCACAAAGAAACTCCATGTGTGGACAAAGTCAACAGTGCCGTTCAATTTTTCAATATTGGCCTCAAGTCCCTTGTTGAGGATCTTAATACCCAATTGCCCGATGCTAAGTTTATCTTCGTTGATGTCTTCCAAATTTCTACTCTTCAGCCTTCAAATATTGGTAAAATGGTTCTAGATGAGCCTTGTTGTGAAGTTCTACCGGGGCAGTTGCAGTGTAGTCCTTTTGGAAAAGTATGTGGCGACAGGACCCAATACCTGTTTTGGGATGGAGTTCATCCAACTGAAATTGGATTTACGACAGTGGCAAGTAGAGCCTTTAATTCTCAACAACCTGGTGATACTCATCCCTTTGATATCAAACACCTTCTTCGCACCTCCTGA

Protein sequence

MKAQLVVYCFLTIAAFGTRGVDGKSIVPCYFVFGDSQADNGNNNILNIGMARADYKPYGIDFSKQQTPTGRFTNGRNVPDFIAKFLGFVGYIPPFENTKGSDILKGVNYASGGSGILPETGRLLGQISSLGKQLENHNHTISKIQALLGRKSRTTEYLKRCLYTVQIGSNDYLNNYFMPLNYKTSFQFTPEHFATTLNKELTRQLKTLYEQGARKVAVFGVGSIGCTPYARANFEHKETPCVDKVNSAVQFFNIGLKSLVEDLNTQLPDAKFIFVDVFQISTLQPSNIGKMVLDEPCCEVLPGQLQCSPFGKVCGDRTQYLFWDGVHPTEIGFTTVASRAFNSQQPGDTHPFDIKHLLRTS
Homology
BLAST of Lag0005732 vs. NCBI nr
Match: XP_038891276.1 (GDSL esterase/lipase At1g29670-like [Benincasa hispida])

HSP 1 Score: 528.5 bits (1360), Expect = 4.6e-146
Identity = 257/372 (69.09%), Postives = 296/372 (79.57%), Query Frame = 0

Query: 1   MKAQL--VVYCFLTIAA---FGTRGVDG-----KSIVPCYFVFGDSQADNGNNNIL---N 60
           MK +L  V YC + I        + VDG     K  V CYFVFGDSQADNGNNN +    
Sbjct: 1   MKGELLMVYYCLIIIIGGLLLENKYVDGASSSSKKKVSCYFVFGDSQADNGNNNDMLERE 60

Query: 61  IGMARADYKPYGIDFSKQQTPTGRFTNGRNVPDFIAKFLGFVGYIPPFENTKGSDILKGV 120
            G ARADYKPYGIDFSKQ  PTGRFTNGRNVPDFIAKFLGF  YIPPF+ TK   ILKGV
Sbjct: 61  YGNARADYKPYGIDFSKQSIPTGRFTNGRNVPDFIAKFLGFRDYIPPFKITKSRTILKGV 120

Query: 121 NYASGGSGILPETGRLLGQISSLGKQLENHNHTISKIQALLGRKSRTTEYLKRCLYTVQI 180
           NYASGG+GIL ETGR LGQ+SS+ KQLENHN+TIS++  LLG K++ T YLKRCLYT+QI
Sbjct: 121 NYASGGAGILRETGRTLGQVSSIKKQLENHNYTISQMHGLLGSKTKITSYLKRCLYTIQI 180

Query: 181 GSNDYLNNYFMPLNYKTSFQFTPEHFATTLNKELTRQLKTLYEQGARKVAVFGVGSIGCT 240
           GSNDYLNN+FMP  Y+TS Q++P+ +A  LNKEL+ QLK LY+QGARKVA+FGVGSIGCT
Sbjct: 181 GSNDYLNNFFMPKLYQTSSQYSPKAYAIALNKELSTQLKILYDQGARKVAIFGVGSIGCT 240

Query: 241 PYARANFEHKETPCVDKVNSAVQFFNIGLKSLVEDLNTQLPDAKFIFVDVFQISTLQPSN 300
           PYARANFEH+  PCVDK+NSA+Q FN GLKSLV+ LNT LP AKF F+DVF+IST+ PS+
Sbjct: 241 PYARANFEHRGLPCVDKMNSAIQLFNDGLKSLVQQLNTNLPAAKFTFIDVFKISTIDPSD 300

Query: 301 IGKMVLDEPCCEVLPGQLQCSPFGKVCGDRTQYLFWDGVHPTEIGFTTVASRAFNSQQPG 360
            GK VLD PCCEV  G +QC PFGKVCG+R  Y+FWDGVHPTEIGF  VASRAFN++Q G
Sbjct: 301 YGKKVLDAPCCEVEVGGMQCDPFGKVCGNRMDYMFWDGVHPTEIGFRLVASRAFNAKQAG 360

BLAST of Lag0005732 vs. NCBI nr
Match: XP_011649033.1 (GDSL esterase/lipase At1g29670 [Cucumis sativus] >XP_031737227.1 GDSL esterase/lipase At1g29670 [Cucumis sativus] >KGN61342.1 hypothetical protein Csa_005903 [Cucumis sativus])

HSP 1 Score: 507.3 bits (1305), Expect = 1.1e-139
Identity = 241/349 (69.05%), Postives = 272/349 (77.94%), Query Frame = 0

Query: 19  RGVDGKSIVPCYFVFGDSQADNGNNNIL---NIGMARADYKPYGIDFSKQQT---PTGRF 78
           +G  G+ I  CYFVFGDSQADNGNNN +     G ARADYKPYGIDFS   +   PTGRF
Sbjct: 37  KGGSGRGICSCYFVFGDSQADNGNNNDMLEREYGRARADYKPYGIDFSSSSSSYIPTGRF 96

Query: 79  TNGRNVPDFIAKFLGFVGYIPPFENTKGSDILKGVNYASGGSGILPETGRLLGQISSLGK 138
           TN RNVPDFIAKFLGF  YIPPF  TK   ILKG NYASGG+GIL ETGR LGQ+SS+ K
Sbjct: 97  TNARNVPDFIAKFLGFDDYIPPFRTTKSRTILKGANYASGGAGILRETGRTLGQVSSIKK 156

Query: 139 QLENHNHTISKIQALLGRKSRTTEYLKRCLYTVQIGSNDYLNNYFMPLNYKTSFQFTPEH 198
           QLENHN+TIS++  LLG  S+   YLK CLYTVQIGSNDYLNNYFMP  Y TS Q++P+ 
Sbjct: 157 QLENHNYTISQMHTLLGSSSKINRYLKECLYTVQIGSNDYLNNYFMPKIYMTSTQYSPQA 216

Query: 199 FATTLNKELTRQLKTLYEQGARKVAVFGVGSIGCTPYARANFEHKETPCVDKVNSAVQFF 258
           FAT LNK L++ LK LY  GARKVA+FGVGSIGCTPYAR NFEH   PCVD++NSA+Q F
Sbjct: 217 FATALNKNLSQHLKNLYNYGARKVAIFGVGSIGCTPYARENFEHTGLPCVDEINSAIQLF 276

Query: 259 NIGLKSLVEDLNTQLPDAKFIFVDVFQISTLQPSNIGKMVLDEPCCEVLPGQLQCSPFGK 318
           N GLKSLV+ LN  LP AKF F+DVFQIST+ P N GKMVLD PCCEV  G +QCSPFGK
Sbjct: 277 NSGLKSLVQHLNANLPSAKFTFIDVFQISTVDPLNYGKMVLDAPCCEVGAGAMQCSPFGK 336

Query: 319 VCGDRTQYLFWDGVHPTEIGFTTVASRAFNSQQPGDTHPFDIKHLLRTS 362
           VC +R  Y+FWDGVHPTE GF  VASRAFN++QPG+ +PFDI HL+  S
Sbjct: 337 VCKNRGDYMFWDGVHPTESGFKLVASRAFNAKQPGEAYPFDINHLVHLS 385

BLAST of Lag0005732 vs. NCBI nr
Match: XP_022144504.1 (GDSL esterase/lipase At1g29670-like [Momordica charantia])

HSP 1 Score: 498.8 bits (1283), Expect = 3.9e-137
Identity = 231/341 (67.74%), Postives = 281/341 (82.40%), Query Frame = 0

Query: 24  KSIVPCYFVFGDSQADNGNNNILNIGMARADYKPYGIDFSKQQ--TPTGRFTNGRNVPDF 83
           KS + CYF+FGDSQ+DNGNNN +  G A A+YKPYGIDF  Q   TPTGRFTNGRN+PDF
Sbjct: 55  KSRMDCYFIFGDSQSDNGNNNDIK-GDAMANYKPYGIDFPNQNSPTPTGRFTNGRNLPDF 114

Query: 84  IAKFLGFVGYIPPFENTKGSDILKGVNYASGGSGILPETGRLLGQISSLGKQLENHNHTI 143
           IA+ LGF  YIPPF NT G ++LKGVNYASGGSGI  ETGR+LG++ S+ KQLENHNHTI
Sbjct: 115 IAELLGFHDYIPPFRNTTGWNVLKGVNYASGGSGIHSETGRMLGKVLSINKQLENHNHTI 174

Query: 144 SKIQALLGRKSRTTEYLKRCLYTVQIGSNDYLNNYFMPLNYKTSFQFTPEHFATTLNKEL 203
           S+IQ  LG K   T++LK CLYTVQ GSNDYLNNYF+P +YKTS QFTP+ +A  LNK+L
Sbjct: 175 SRIQGSLGSKLAATKHLKSCLYTVQAGSNDYLNNYFLPFSYKTSAQFTPQQYAVALNKQL 234

Query: 204 TRQLKTLYEQGARKVAVFGVGSIGCTPYARANFEHKETPCVDKVNSAVQFFNIGLKSLVE 263
           + QL+ LY++G RKVAVFGVG IGCTPYARANFEH+ +PCV+K+N A+Q FN GLKSL+ 
Sbjct: 235 SHQLEELYDRGGRKVAVFGVGGIGCTPYARANFEHEGSPCVEKINYAIQLFNSGLKSLIH 294

Query: 264 DLNTQLPDAKFIFVDVFQISTLQPSNI---GKMVLDEPCCEVLPGQLQCSPFGKVCGDRT 323
           DLNT+  DAKFIF+DVF+IST++P+ +     +VLD PCCEV  GQLQC+PFGKVCG+R+
Sbjct: 295 DLNTKFTDAKFIFIDVFKISTMKPTPLVPADLLVLDAPCCEVPVGQLQCTPFGKVCGNRS 354

Query: 324 QYLFWDGVHPTEIGFTTVASRAFNSQQPGDTHPFDIKHLLR 360
           +Y+FWDGVHPTE+GF  VA RAFN+++P DT+PFDI HL+R
Sbjct: 355 KYMFWDGVHPTELGFKLVAKRAFNAKEPDDTYPFDISHLVR 394

BLAST of Lag0005732 vs. NCBI nr
Match: XP_008441762.1 (PREDICTED: GDSL esterase/lipase At1g29670-like [Cucumis melo] >KAA0051383.1 GDSL esterase/lipase [Cucumis melo var. makuwa] >TYK23839.1 GDSL esterase/lipase [Cucumis melo var. makuwa])

HSP 1 Score: 496.1 bits (1276), Expect = 2.5e-136
Identity = 237/351 (67.52%), Postives = 273/351 (77.78%), Query Frame = 0

Query: 19  RGVDGKSIVPCYFVFGDSQADNGNNNIL---NIGMARADYKPYGIDF-----SKQQTPTG 78
           +G  G     CYFVFGDSQADNGNNN +     G ARADYKPYGIDF     S    PTG
Sbjct: 36  KGGGGSDACSCYFVFGDSQADNGNNNDMLEREYGRARADYKPYGIDFPPSSSSSSFIPTG 95

Query: 79  RFTNGRNVPDFIAKFLGFVGYIPPFENTKGSDILKGVNYASGGSGILPETGRLLGQISSL 138
           RFTNGRNVPDFIA+FLGF  YIPPF  TK   ILKG NYASGG+GIL ETGR LG++SS+
Sbjct: 96  RFTNGRNVPDFIAQFLGFRDYIPPFRTTKSQTILKGANYASGGAGILLETGRTLGEVSSI 155

Query: 139 GKQLENHNHTISKIQALLGRKSRTTEYLKRCLYTVQIGSNDYLNNYFMPLNYKTSFQFTP 198
            KQLENHN+TIS++Q L G  S+   YLK CLYTVQIGSNDYLNNYFMP  Y TS Q++P
Sbjct: 156 KKQLENHNYTISQMQRLFGSSSKLKSYLKECLYTVQIGSNDYLNNYFMPTIYMTSTQYSP 215

Query: 199 EHFATTLNKELTRQLKTLYEQGARKVAVFGVGSIGCTPYARANFEHKETPCVDKVNSAVQ 258
           + FAT LN++L+RQLK LYEQGARKVA+FGVGSIGCTPYARANFEH   PCVDK+N+A+Q
Sbjct: 216 QAFATALNEDLSRQLKILYEQGARKVAIFGVGSIGCTPYARANFEHIGLPCVDKINTAIQ 275

Query: 259 FFNIGLKSLVEDLNTQLPDAKFIFVDVFQISTLQPSNIGKMVLDEPCCEVLPGQLQCSPF 318
            FN GLKSLVE LN  LP AKF F+DVF+IST+ P N GK+ LD PCCEV  G++QC+PF
Sbjct: 276 LFNAGLKSLVEHLNANLPSAKFTFIDVFKISTVDPLNYGKVNLDAPCCEVGAGEMQCTPF 335

Query: 319 GKVCGDRTQYLFWDGVHPTEIGFTTVASRAFNSQQPGDTHPFDIKHLLRTS 362
           GKVC +R  Y+F+DGVHPTE GF  VAS AFN++QP + +PFDI HL+  S
Sbjct: 336 GKVCENRRDYMFFDGVHPTENGFELVASSAFNAKQPDEAYPFDINHLVHLS 386

BLAST of Lag0005732 vs. NCBI nr
Match: XP_022973923.1 (GDSL esterase/lipase At1g29670-like [Cucurbita maxima])

HSP 1 Score: 485.7 bits (1249), Expect = 3.4e-133
Identity = 236/380 (62.11%), Postives = 285/380 (75.00%), Query Frame = 0

Query: 1   MKAQ-LVVYCFLTIAAFGTRGVDG-------------------KSIVPCYFVFGDSQADN 60
           MK Q LVV C LT A F +  VDG                   K+ V CYFV GDSQADN
Sbjct: 1   MKGQSLVVLCLLTTACFASSWVDGASPRIKSRTRPKSNATTQTKTKVGCYFVLGDSQADN 60

Query: 61  GNNNILNI--GMARADYKPYGIDFSKQQTPTGRFTNGRNVPDFIAKFLGFVGYIPPFENT 120
           GNNN++ +  G ARADYKPYG DF+++ TPTGRFTNGRN PDFIA +LGF  YI PF   
Sbjct: 61  GNNNVMEMAYGNARADYKPYGCDFNREGTPTGRFTNGRNAPDFIATYLGFRHYISPFTTI 120

Query: 121 KGSDILKGVNYASGGSGILPETGRLLGQISSLGKQLENHNHTISKIQALLGRKSRTTEYL 180
           KG +IL GVNYASGG+GILPETGR LGQ+ S+ KQLENHN TIS+I+ LLG +S TT +L
Sbjct: 121 KGREILDGVNYASGGAGILPETGRTLGQVLSIHKQLENHNITISQIRGLLGTRSATTTHL 180

Query: 181 KRCLYTVQIGSNDYLNNYFMPLNYKTSFQFTPEHFATTLNKELTRQLKTLYEQGARKVAV 240
             CLYTVQIGSNDYLNNYFMP  YKTS QFTP+ FAT LN  L  +LK LYEQGARKVA+
Sbjct: 181 NSCLYTVQIGSNDYLNNYFMPQFYKTSAQFTPQQFATVLNNRLYTELKVLYEQGARKVAL 240

Query: 241 FGVGSIGCTPYARANFEHKETPCVDKVNSAVQFFNIGLKSLVEDLNTQLPDAKFIFVDVF 300
           FGVGSIGCTPYARANFE+K   CVDK+N+A+Q FN GLK+LV  LNT++  AKF ++DVF
Sbjct: 241 FGVGSIGCTPYARANFENKGGGCVDKINNAIQLFNDGLKALVLQLNTKMAGAKFTYIDVF 300

Query: 301 QISTLQPSNIGKMVLDEPCCEVLPGQLQCSPFGKVCGDRTQYLFWDGVHPTEIGFTTVAS 359
           +IS+  PS  G+MVL+ PCCEV  GQ+QC+  G++CG+R +Y+FWD VHPTE+G + +AS
Sbjct: 301 KISSTIPSTSGRMVLNAPCCEVDAGQVQCTRLGRICGNRMEYMFWDAVHPTEVGISALAS 360

BLAST of Lag0005732 vs. ExPASy Swiss-Prot
Match: Q9C7N4 (GDSL esterase/lipase At1g29670 OS=Arabidopsis thaliana OX=3702 GN=At1g29670 PE=2 SV=1)

HSP 1 Score: 334.7 bits (857), Expect = 1.3e-90
Identity = 172/358 (48.04%), Postives = 222/358 (62.01%), Query Frame = 0

Query: 5   LVVYCFLTIAAFGTRGVDGKSIVPCYFVFGDSQADNGNNNILNIGMARADYKPYGIDFSK 64
           LV+ CF     F       ++ VPC+FVFGDS  DNGNNN L I +AR++Y PYGIDF  
Sbjct: 12  LVLLCF----GFSVVKAQAQAQVPCFFVFGDSLVDNGNNNGL-ISIARSNYFPYGIDFG- 71

Query: 65  QQTPTGRFTNGRNVPDFIAKFLGFVGYIPPFENTKGSDILKGVNYASGGSGILPETGRLL 124
              PTGRF+NG+   D IA+ LGF GYIP +    G  IL GVNYAS  +GI  ETGR L
Sbjct: 72  --GPTGRFSNGKTTVDVIAELLGFNGYIPAYNTVSGRQILSGVNYASAAAGIREETGRQL 131

Query: 125 GQISSLGKQLENHNHTISKIQALLGRKSRTTEYLKRCLYTVQIGSNDYLNNYFMPLNYKT 184
           GQ  S   Q+ N+  T+S++  LLG ++R  +YLKRC+Y+V +GSNDYLNNYFMP  Y +
Sbjct: 132 GQRISFSGQVRNYQTTVSQVVQLLGDETRAADYLKRCIYSVGLGSNDYLNNYFMPTFYSS 191

Query: 185 SFQFTPEHFATTLNKELTRQLKTLYEQGARKVAVFGVGSIGCTPYARANFEHKETPCVDK 244
           S QFTPE +A  L    + QL  LY  GARK A+ G+G++GC+P A A      T CVD+
Sbjct: 192 SRQFTPEQYANDLISRYSTQLNALYNYGARKFALSGIGAVGCSPNALAGSPDGRT-CVDR 251

Query: 245 VNSAVQFFNIGLKSLVEDLNTQLPDAKFIFVD---VFQISTLQPSNIGKMVLDEPCCEV- 304
           +NSA Q FN  L+SLV+ LN   PDAKFI+++   +FQ     P+  G  V +  CC + 
Sbjct: 252 INSANQIFNNKLRSLVDQLNNNHPDAKFIYINAYGIFQDMITNPARFGFRVTNAGCCGIG 311

Query: 305 -LPGQLQCSPFGKVCGDRTQYLFWDGVHPTEIGFTTVASRAFNSQQPGDTHPFDIKHL 358
              GQ+ C P  + C DR  Y+FWD  HPTE     +A R++N+Q   D +P DI  L
Sbjct: 312 RNAGQITCLPGQRPCRDRNAYVFWDAFHPTEAANVIIARRSYNAQSASDAYPMDISRL 360

BLAST of Lag0005732 vs. ExPASy Swiss-Prot
Match: Q93YW8 (GDSL esterase/lipase At4g18970 OS=Arabidopsis thaliana OX=3702 GN=At4g18970 PE=2 SV=1)

HSP 1 Score: 312.0 bits (798), Expect = 8.8e-84
Identity = 152/342 (44.44%), Postives = 207/342 (60.53%), Query Frame = 0

Query: 23  GKSIVPCYFVFGDSQADNGNNNILNIGMARADYKPYGIDFSKQQTPTGRFTNGRNVPDFI 82
           G  I PCYF+FGDS  D+GNNN L   +ARA+Y PYGIDF  Q  PTGRF+NG+   D I
Sbjct: 22  GDPIAPCYFIFGDSLVDSGNNNRLT-SLARANYFPYGIDF--QYGPTGRFSNGKTTVDVI 81

Query: 83  AKFLGFVGYIPPFENTKGSDILKGVNYASGGSGILPETGRLLGQISSLGKQLENHNHTIS 142
            + LGF  YI P+   +G DIL+GVNYAS  +GI  ETGR LG   +   Q+ NH +T+S
Sbjct: 82  TELLGFDDYITPYSEARGEDILRGVNYASAAAGIREETGRQLGARITFAGQVANHVNTVS 141

Query: 143 KIQALLGRKSRTTEYLKRCLYTVQIGSNDYLNNYFMPLNYKTSFQFTPEHFATTLNKELT 202
           ++  +LG ++    YL +C+Y++ +GSNDYLNNYFMP+ Y T  Q++P+ +A  L    T
Sbjct: 142 QVVNILGDENEAANYLSKCIYSIGLGSNDYLNNYFMPVYYSTGSQYSPDAYANDLINRYT 201

Query: 203 RQLKTLYEQGARKVAVFGVGSIGCTPYARANFEHKETPCVDKVNSAVQFFNIGLKSLVED 262
            QL+ +Y  GARK A+ G+G+IGC+P   A        C +++NSA + FN  L SLV+ 
Sbjct: 202 EQLRIMYNNGARKFALVGIGAIGCSPNELAQNSRDGVTCDERINSANRIFNSKLVSLVDH 261

Query: 263 LNTQLPDAKFIFVD---VFQISTLQPSNIGKMVLDEPCCEV--LPGQLQCSPFGKVCGDR 322
            N   P AKF +++   +FQ     PS  G  V +  CC V    GQ+ C P    C +R
Sbjct: 262 FNQNTPGAKFTYINAYGIFQDMVANPSRYGFRVTNAGCCGVGRNNGQITCLPGQAPCLNR 321

Query: 323 TQYLFWDGVHPTEIGFTTVASRAFNSQQPGDTHPFDIKHLLR 360
            +Y+FWD  HP E     + SR+F  +   D HP+DI+ L R
Sbjct: 322 DEYVFWDAFHPGEAANVVIGSRSFQRESASDAHPYDIQQLAR 360

BLAST of Lag0005732 vs. ExPASy Swiss-Prot
Match: Q9C7N5 (GDSL esterase/lipase At1g29660 OS=Arabidopsis thaliana OX=3702 GN=At1g29660 PE=1 SV=1)

HSP 1 Score: 309.7 bits (792), Expect = 4.4e-83
Identity = 156/336 (46.43%), Postives = 206/336 (61.31%), Query Frame = 0

Query: 27  VPCYFVFGDSQADNGNNNILNIGMARADYKPYGIDFSKQQTPTGRFTNGRNVPDFIAKFL 86
           VPCYF+FGDS  DNGNNN L   +ARADY PYGIDF     PTGRF+NGR   D + + L
Sbjct: 30  VPCYFIFGDSLVDNGNNNRLR-SIARADYFPYGIDFG---GPTGRFSNGRTTVDVLTELL 89

Query: 87  GFVGYIPPFENTKGSDILKGVNYASGGSGILPETGRLLGQISSLGKQLENHNHTISKIQA 146
           GF  YIP +    G +IL+GVNYAS  +GI  ETG  LGQ  +   Q+EN+ +T++++  
Sbjct: 90  GFDNYIPAYSTVSGQEILQGVNYASAAAGIREETGAQLGQRITFSGQVENYKNTVAQVVE 149

Query: 147 LLGRKSRTTEYLKRCLYTVQIGSNDYLNNYFMPLNYKTSFQFTPEHFATTLNKELTRQLK 206
           +LG +    +YLKRC+Y+V +GSNDYLNNYFMP  Y TS Q+TPE +A  L      QL 
Sbjct: 150 ILGDEYTAADYLKRCIYSVGMGSNDYLNNYFMPQFYSTSRQYTPEQYADDLISRYRDQLN 209

Query: 207 TLYEQGARKVAVFGVGSIGCTPYARANFEHKETPCVDKVNSAVQFFNIGLKSLVEDLNTQ 266
            LY  GARK A+ G+G+IGC+P A A      T CV+++NSA + FN  L S+V+ LN  
Sbjct: 210 ALYNYGARKFALVGIGAIGCSPNALAQGSQDGTTCVERINSANRIFNNRLISMVQQLNNA 269

Query: 267 LPDAKFIFVD---VFQISTLQPSNIGKMVLDEPCCEV--LPGQLQCSPFGKVCGDRTQYL 326
             DA F +++    FQ     PS  G    +  CC +    GQL C P    C +R +Y+
Sbjct: 270 HSDASFTYINAYGAFQDIIANPSAYGFTNTNTACCGIGRNGGQLTCLPGEPPCLNRDEYV 329

Query: 327 FWDGVHPTEIGFTTVASRAFNSQQPGDTHPFDIKHL 358
           FWD  HP+    T +A R++N+Q+  D +P DI  L
Sbjct: 330 FWDAFHPSAAANTAIAKRSYNAQRSSDVYPIDISQL 361

BLAST of Lag0005732 vs. ExPASy Swiss-Prot
Match: Q9FK75 (GDSL esterase/lipase At5g45670 OS=Arabidopsis thaliana OX=3702 GN=At5g45670 PE=2 SV=1)

HSP 1 Score: 307.4 bits (786), Expect = 2.2e-82
Identity = 153/337 (45.40%), Postives = 206/337 (61.13%), Query Frame = 0

Query: 26  IVPCYFVFGDSQADNGNNNILNIGMARADYKPYGIDFSKQQTPTGRFTNGRNVPDFIAKF 85
           I PCYF+FGDS  DNGNNN L   +ARA+Y PYGIDF+    PTGRF+NG    D IA+ 
Sbjct: 26  IAPCYFIFGDSLVDNGNNNQLQ-SLARANYFPYGIDFA--AGPTGRFSNGLTTVDVIAQL 85

Query: 86  LGFVGYIPPFENTKGSDILKGVNYASGGSGILPETGRLLGQISSLGKQLENHNHTISKIQ 145
           LGF  YI P+ + +G DIL+GVNYAS  +GI  ETGR LG   +   Q+ NH +T+S++ 
Sbjct: 86  LGFEDYITPYASARGQDILRGVNYASAAAGIRDETGRQLGGRIAFAGQVANHVNTVSQVV 145

Query: 146 ALLGRKSRTTEYLKRCLYTVQIGSNDYLNNYFMPLNYKTSFQFTPEHFATTLNKELTRQL 205
            +LG ++  + YL +C+Y++ +GSNDYLNNYFMP  Y T  QF+PE +A  L    T QL
Sbjct: 146 NILGDQNEASNYLSKCIYSIGLGSNDYLNNYFMPTFYSTGNQFSPESYADDLVARYTEQL 205

Query: 206 KTLYEQGARKVAVFGVGSIGCTPYARANFEHKETPCVDKVNSAVQFFNIGLKSLVEDLNT 265
           + LY  GARK A+ GVG+IGC+P   A        C +++NSA + FN  L S+V+  N 
Sbjct: 206 RVLYTNGARKFALIGVGAIGCSPNELAQNSRDGRTCDERINSANRIFNSKLISIVDAFNQ 265

Query: 266 QLPDAKFIFVD---VFQISTLQPSNIGKMVLDEPCCEV--LPGQLQCSPFGKVCGDRTQY 325
             PDAKF +++   +FQ     P+  G  V +  CC V    GQ+ C P    C +R +Y
Sbjct: 266 NTPDAKFTYINAYGIFQDIITNPARYGFRVTNAGCCGVGRNNGQITCLPGQAPCLNRNEY 325

Query: 326 LFWDGVHPTEIGFTTVASRAFNSQQPGDTHPFDIKHL 358
           +FWD  HP E     +  R+F  +   D HP+DI+ L
Sbjct: 326 VFWDAFHPGEAANIVIGRRSFKREAASDAHPYDIQQL 359

BLAST of Lag0005732 vs. ExPASy Swiss-Prot
Match: O64468 (GDSL esterase/lipase At2g19050 OS=Arabidopsis thaliana OX=3702 GN=At2g19050 PE=3 SV=1)

HSP 1 Score: 285.4 bits (729), Expect = 8.9e-76
Identity = 161/359 (44.85%), Postives = 206/359 (57.38%), Query Frame = 0

Query: 2   KAQLVVYCFLTIAAFGTRGVDGKSIVPCYFVFGDSQADNGNNNILNIGMARADYKPYGID 61
           KA L+V   +   AF T        VPCYFVFGDS  DNGNNN+LN   A+ +Y PYGID
Sbjct: 7   KALLLV---IATTAFATTEAALGQRVPCYFVFGDSVFDNGNNNVLNTS-AKVNYSPYGID 66

Query: 62  FSKQQTPTGRFTNGRNVPDFIAKFLGFVGYIPPFENTKGSDILKGVNYASGGSGILPETG 121
           F++   PTGRF+NGRN+PD IA+ + F  YIPPF          G+NYASGG GI  ET 
Sbjct: 67  FAR--GPTGRFSNGRNIPDIIAELMRFSDYIPPFTGASPEQAHIGINYASGGGGIREETS 126

Query: 122 RLLGQISSLGKQLENHNHTISKIQALLGRKSRTTEYLKRCLYTVQIGSNDYLNNYFMPLN 181
           + LG+I S  KQ++NH   I   +          E L +CLYT+ IGSNDYLNNYFMP  
Sbjct: 127 QHLGEIISFKKQIKNHRSMIMTAKV-------PEEKLNKCLYTINIGSNDYLNNYFMPAP 186

Query: 182 YKTSFQFTPEHFATTLNKELTRQLKTLYEQGARKVAVFGVGSIGCTPYARANFEHKETPC 241
           Y T+ +F+ + +A +L +     LK+LY  GARKVAVFGV  +GCTP   A+       C
Sbjct: 187 YMTNKKFSFDEYADSLIRSYRSYLKSLYVLGARKVAVFGVSKLGCTPRMIAS-HGGGNGC 246

Query: 242 VDKVNSAVQFFNIGLKSLVEDLNTQLPDAKFIFVDVFQ-ISTLQPSNIGKMVLDEPCCEV 301
             +VN AV+ FN  LK+LV + N    DAKF FVD+F   S      +G  V D+ CC V
Sbjct: 247 AAEVNKAVEPFNKNLKALVYEFNRDFADAKFTFVDIFSGQSPFAFFMLGFRVTDKSCCTV 306

Query: 302 LPGQLQCSPFGKVCGDRTQYLFWDGVHPTEIGFTTVASRAFNSQQPGDTHPFDIKHLLR 360
            PG+  C+    VC  + +Y++WD VH TE     VA  A+       T P+ +  L R
Sbjct: 307 KPGEELCATNEPVCPVQRRYVYWDNVHSTEAANMVVAKAAYAGL---ITSPYSLSWLAR 348

BLAST of Lag0005732 vs. ExPASy TrEMBL
Match: A0A0A0LKR6 (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_2G093840 PE=3 SV=1)

HSP 1 Score: 507.3 bits (1305), Expect = 5.3e-140
Identity = 241/349 (69.05%), Postives = 272/349 (77.94%), Query Frame = 0

Query: 19  RGVDGKSIVPCYFVFGDSQADNGNNNIL---NIGMARADYKPYGIDFSKQQT---PTGRF 78
           +G  G+ I  CYFVFGDSQADNGNNN +     G ARADYKPYGIDFS   +   PTGRF
Sbjct: 37  KGGSGRGICSCYFVFGDSQADNGNNNDMLEREYGRARADYKPYGIDFSSSSSSYIPTGRF 96

Query: 79  TNGRNVPDFIAKFLGFVGYIPPFENTKGSDILKGVNYASGGSGILPETGRLLGQISSLGK 138
           TN RNVPDFIAKFLGF  YIPPF  TK   ILKG NYASGG+GIL ETGR LGQ+SS+ K
Sbjct: 97  TNARNVPDFIAKFLGFDDYIPPFRTTKSRTILKGANYASGGAGILRETGRTLGQVSSIKK 156

Query: 139 QLENHNHTISKIQALLGRKSRTTEYLKRCLYTVQIGSNDYLNNYFMPLNYKTSFQFTPEH 198
           QLENHN+TIS++  LLG  S+   YLK CLYTVQIGSNDYLNNYFMP  Y TS Q++P+ 
Sbjct: 157 QLENHNYTISQMHTLLGSSSKINRYLKECLYTVQIGSNDYLNNYFMPKIYMTSTQYSPQA 216

Query: 199 FATTLNKELTRQLKTLYEQGARKVAVFGVGSIGCTPYARANFEHKETPCVDKVNSAVQFF 258
           FAT LNK L++ LK LY  GARKVA+FGVGSIGCTPYAR NFEH   PCVD++NSA+Q F
Sbjct: 217 FATALNKNLSQHLKNLYNYGARKVAIFGVGSIGCTPYARENFEHTGLPCVDEINSAIQLF 276

Query: 259 NIGLKSLVEDLNTQLPDAKFIFVDVFQISTLQPSNIGKMVLDEPCCEVLPGQLQCSPFGK 318
           N GLKSLV+ LN  LP AKF F+DVFQIST+ P N GKMVLD PCCEV  G +QCSPFGK
Sbjct: 277 NSGLKSLVQHLNANLPSAKFTFIDVFQISTVDPLNYGKMVLDAPCCEVGAGAMQCSPFGK 336

Query: 319 VCGDRTQYLFWDGVHPTEIGFTTVASRAFNSQQPGDTHPFDIKHLLRTS 362
           VC +R  Y+FWDGVHPTE GF  VASRAFN++QPG+ +PFDI HL+  S
Sbjct: 337 VCKNRGDYMFWDGVHPTESGFKLVASRAFNAKQPGEAYPFDINHLVHLS 385

BLAST of Lag0005732 vs. ExPASy TrEMBL
Match: A0A6J1CSH4 (GDSL esterase/lipase At1g29670-like OS=Momordica charantia OX=3673 GN=LOC111014175 PE=3 SV=1)

HSP 1 Score: 498.8 bits (1283), Expect = 1.9e-137
Identity = 231/341 (67.74%), Postives = 281/341 (82.40%), Query Frame = 0

Query: 24  KSIVPCYFVFGDSQADNGNNNILNIGMARADYKPYGIDFSKQQ--TPTGRFTNGRNVPDF 83
           KS + CYF+FGDSQ+DNGNNN +  G A A+YKPYGIDF  Q   TPTGRFTNGRN+PDF
Sbjct: 55  KSRMDCYFIFGDSQSDNGNNNDIK-GDAMANYKPYGIDFPNQNSPTPTGRFTNGRNLPDF 114

Query: 84  IAKFLGFVGYIPPFENTKGSDILKGVNYASGGSGILPETGRLLGQISSLGKQLENHNHTI 143
           IA+ LGF  YIPPF NT G ++LKGVNYASGGSGI  ETGR+LG++ S+ KQLENHNHTI
Sbjct: 115 IAELLGFHDYIPPFRNTTGWNVLKGVNYASGGSGIHSETGRMLGKVLSINKQLENHNHTI 174

Query: 144 SKIQALLGRKSRTTEYLKRCLYTVQIGSNDYLNNYFMPLNYKTSFQFTPEHFATTLNKEL 203
           S+IQ  LG K   T++LK CLYTVQ GSNDYLNNYF+P +YKTS QFTP+ +A  LNK+L
Sbjct: 175 SRIQGSLGSKLAATKHLKSCLYTVQAGSNDYLNNYFLPFSYKTSAQFTPQQYAVALNKQL 234

Query: 204 TRQLKTLYEQGARKVAVFGVGSIGCTPYARANFEHKETPCVDKVNSAVQFFNIGLKSLVE 263
           + QL+ LY++G RKVAVFGVG IGCTPYARANFEH+ +PCV+K+N A+Q FN GLKSL+ 
Sbjct: 235 SHQLEELYDRGGRKVAVFGVGGIGCTPYARANFEHEGSPCVEKINYAIQLFNSGLKSLIH 294

Query: 264 DLNTQLPDAKFIFVDVFQISTLQPSNI---GKMVLDEPCCEVLPGQLQCSPFGKVCGDRT 323
           DLNT+  DAKFIF+DVF+IST++P+ +     +VLD PCCEV  GQLQC+PFGKVCG+R+
Sbjct: 295 DLNTKFTDAKFIFIDVFKISTMKPTPLVPADLLVLDAPCCEVPVGQLQCTPFGKVCGNRS 354

Query: 324 QYLFWDGVHPTEIGFTTVASRAFNSQQPGDTHPFDIKHLLR 360
           +Y+FWDGVHPTE+GF  VA RAFN+++P DT+PFDI HL+R
Sbjct: 355 KYMFWDGVHPTELGFKLVAKRAFNAKEPDDTYPFDISHLVR 394

BLAST of Lag0005732 vs. ExPASy TrEMBL
Match: A0A5D3DKM8 (GDSL esterase/lipase OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold419G00010 PE=3 SV=1)

HSP 1 Score: 496.1 bits (1276), Expect = 1.2e-136
Identity = 237/351 (67.52%), Postives = 273/351 (77.78%), Query Frame = 0

Query: 19  RGVDGKSIVPCYFVFGDSQADNGNNNIL---NIGMARADYKPYGIDF-----SKQQTPTG 78
           +G  G     CYFVFGDSQADNGNNN +     G ARADYKPYGIDF     S    PTG
Sbjct: 36  KGGGGSDACSCYFVFGDSQADNGNNNDMLEREYGRARADYKPYGIDFPPSSSSSSFIPTG 95

Query: 79  RFTNGRNVPDFIAKFLGFVGYIPPFENTKGSDILKGVNYASGGSGILPETGRLLGQISSL 138
           RFTNGRNVPDFIA+FLGF  YIPPF  TK   ILKG NYASGG+GIL ETGR LG++SS+
Sbjct: 96  RFTNGRNVPDFIAQFLGFRDYIPPFRTTKSQTILKGANYASGGAGILLETGRTLGEVSSI 155

Query: 139 GKQLENHNHTISKIQALLGRKSRTTEYLKRCLYTVQIGSNDYLNNYFMPLNYKTSFQFTP 198
            KQLENHN+TIS++Q L G  S+   YLK CLYTVQIGSNDYLNNYFMP  Y TS Q++P
Sbjct: 156 KKQLENHNYTISQMQRLFGSSSKLKSYLKECLYTVQIGSNDYLNNYFMPTIYMTSTQYSP 215

Query: 199 EHFATTLNKELTRQLKTLYEQGARKVAVFGVGSIGCTPYARANFEHKETPCVDKVNSAVQ 258
           + FAT LN++L+RQLK LYEQGARKVA+FGVGSIGCTPYARANFEH   PCVDK+N+A+Q
Sbjct: 216 QAFATALNEDLSRQLKILYEQGARKVAIFGVGSIGCTPYARANFEHIGLPCVDKINTAIQ 275

Query: 259 FFNIGLKSLVEDLNTQLPDAKFIFVDVFQISTLQPSNIGKMVLDEPCCEVLPGQLQCSPF 318
            FN GLKSLVE LN  LP AKF F+DVF+IST+ P N GK+ LD PCCEV  G++QC+PF
Sbjct: 276 LFNAGLKSLVEHLNANLPSAKFTFIDVFKISTVDPLNYGKVNLDAPCCEVGAGEMQCTPF 335

Query: 319 GKVCGDRTQYLFWDGVHPTEIGFTTVASRAFNSQQPGDTHPFDIKHLLRTS 362
           GKVC +R  Y+F+DGVHPTE GF  VAS AFN++QP + +PFDI HL+  S
Sbjct: 336 GKVCENRRDYMFFDGVHPTENGFELVASSAFNAKQPDEAYPFDINHLVHLS 386

BLAST of Lag0005732 vs. ExPASy TrEMBL
Match: A0A1S3B473 (GDSL esterase/lipase At1g29670-like OS=Cucumis melo OX=3656 GN=LOC103485821 PE=3 SV=1)

HSP 1 Score: 496.1 bits (1276), Expect = 1.2e-136
Identity = 237/351 (67.52%), Postives = 273/351 (77.78%), Query Frame = 0

Query: 19  RGVDGKSIVPCYFVFGDSQADNGNNNIL---NIGMARADYKPYGIDF-----SKQQTPTG 78
           +G  G     CYFVFGDSQADNGNNN +     G ARADYKPYGIDF     S    PTG
Sbjct: 36  KGGGGSDACSCYFVFGDSQADNGNNNDMLEREYGRARADYKPYGIDFPPSSSSSSFIPTG 95

Query: 79  RFTNGRNVPDFIAKFLGFVGYIPPFENTKGSDILKGVNYASGGSGILPETGRLLGQISSL 138
           RFTNGRNVPDFIA+FLGF  YIPPF  TK   ILKG NYASGG+GIL ETGR LG++SS+
Sbjct: 96  RFTNGRNVPDFIAQFLGFRDYIPPFRTTKSQTILKGANYASGGAGILLETGRTLGEVSSI 155

Query: 139 GKQLENHNHTISKIQALLGRKSRTTEYLKRCLYTVQIGSNDYLNNYFMPLNYKTSFQFTP 198
            KQLENHN+TIS++Q L G  S+   YLK CLYTVQIGSNDYLNNYFMP  Y TS Q++P
Sbjct: 156 KKQLENHNYTISQMQRLFGSSSKLKSYLKECLYTVQIGSNDYLNNYFMPTIYMTSTQYSP 215

Query: 199 EHFATTLNKELTRQLKTLYEQGARKVAVFGVGSIGCTPYARANFEHKETPCVDKVNSAVQ 258
           + FAT LN++L+RQLK LYEQGARKVA+FGVGSIGCTPYARANFEH   PCVDK+N+A+Q
Sbjct: 216 QAFATALNEDLSRQLKILYEQGARKVAIFGVGSIGCTPYARANFEHIGLPCVDKINTAIQ 275

Query: 259 FFNIGLKSLVEDLNTQLPDAKFIFVDVFQISTLQPSNIGKMVLDEPCCEVLPGQLQCSPF 318
            FN GLKSLVE LN  LP AKF F+DVF+IST+ P N GK+ LD PCCEV  G++QC+PF
Sbjct: 276 LFNAGLKSLVEHLNANLPSAKFTFIDVFKISTVDPLNYGKVNLDAPCCEVGAGEMQCTPF 335

Query: 319 GKVCGDRTQYLFWDGVHPTEIGFTTVASRAFNSQQPGDTHPFDIKHLLRTS 362
           GKVC +R  Y+F+DGVHPTE GF  VAS AFN++QP + +PFDI HL+  S
Sbjct: 336 GKVCENRRDYMFFDGVHPTENGFELVASSAFNAKQPDEAYPFDINHLVHLS 386

BLAST of Lag0005732 vs. ExPASy TrEMBL
Match: A0A6J1IEL6 (GDSL esterase/lipase At1g29670-like OS=Cucurbita maxima OX=3661 GN=LOC111472555 PE=3 SV=1)

HSP 1 Score: 485.7 bits (1249), Expect = 1.6e-133
Identity = 236/380 (62.11%), Postives = 285/380 (75.00%), Query Frame = 0

Query: 1   MKAQ-LVVYCFLTIAAFGTRGVDG-------------------KSIVPCYFVFGDSQADN 60
           MK Q LVV C LT A F +  VDG                   K+ V CYFV GDSQADN
Sbjct: 1   MKGQSLVVLCLLTTACFASSWVDGASPRIKSRTRPKSNATTQTKTKVGCYFVLGDSQADN 60

Query: 61  GNNNILNI--GMARADYKPYGIDFSKQQTPTGRFTNGRNVPDFIAKFLGFVGYIPPFENT 120
           GNNN++ +  G ARADYKPYG DF+++ TPTGRFTNGRN PDFIA +LGF  YI PF   
Sbjct: 61  GNNNVMEMAYGNARADYKPYGCDFNREGTPTGRFTNGRNAPDFIATYLGFRHYISPFTTI 120

Query: 121 KGSDILKGVNYASGGSGILPETGRLLGQISSLGKQLENHNHTISKIQALLGRKSRTTEYL 180
           KG +IL GVNYASGG+GILPETGR LGQ+ S+ KQLENHN TIS+I+ LLG +S TT +L
Sbjct: 121 KGREILDGVNYASGGAGILPETGRTLGQVLSIHKQLENHNITISQIRGLLGTRSATTTHL 180

Query: 181 KRCLYTVQIGSNDYLNNYFMPLNYKTSFQFTPEHFATTLNKELTRQLKTLYEQGARKVAV 240
             CLYTVQIGSNDYLNNYFMP  YKTS QFTP+ FAT LN  L  +LK LYEQGARKVA+
Sbjct: 181 NSCLYTVQIGSNDYLNNYFMPQFYKTSAQFTPQQFATVLNNRLYTELKVLYEQGARKVAL 240

Query: 241 FGVGSIGCTPYARANFEHKETPCVDKVNSAVQFFNIGLKSLVEDLNTQLPDAKFIFVDVF 300
           FGVGSIGCTPYARANFE+K   CVDK+N+A+Q FN GLK+LV  LNT++  AKF ++DVF
Sbjct: 241 FGVGSIGCTPYARANFENKGGGCVDKINNAIQLFNDGLKALVLQLNTKMAGAKFTYIDVF 300

Query: 301 QISTLQPSNIGKMVLDEPCCEVLPGQLQCSPFGKVCGDRTQYLFWDGVHPTEIGFTTVAS 359
           +IS+  PS  G+MVL+ PCCEV  GQ+QC+  G++CG+R +Y+FWD VHPTE+G + +AS
Sbjct: 301 KISSTIPSTSGRMVLNAPCCEVDAGQVQCTRLGRICGNRMEYMFWDAVHPTEVGISALAS 360

BLAST of Lag0005732 vs. TAIR 10
Match: AT1G29670.1 (GDSL-like Lipase/Acylhydrolase superfamily protein )

HSP 1 Score: 334.7 bits (857), Expect = 9.0e-92
Identity = 172/358 (48.04%), Postives = 222/358 (62.01%), Query Frame = 0

Query: 5   LVVYCFLTIAAFGTRGVDGKSIVPCYFVFGDSQADNGNNNILNIGMARADYKPYGIDFSK 64
           LV+ CF     F       ++ VPC+FVFGDS  DNGNNN L I +AR++Y PYGIDF  
Sbjct: 12  LVLLCF----GFSVVKAQAQAQVPCFFVFGDSLVDNGNNNGL-ISIARSNYFPYGIDFG- 71

Query: 65  QQTPTGRFTNGRNVPDFIAKFLGFVGYIPPFENTKGSDILKGVNYASGGSGILPETGRLL 124
              PTGRF+NG+   D IA+ LGF GYIP +    G  IL GVNYAS  +GI  ETGR L
Sbjct: 72  --GPTGRFSNGKTTVDVIAELLGFNGYIPAYNTVSGRQILSGVNYASAAAGIREETGRQL 131

Query: 125 GQISSLGKQLENHNHTISKIQALLGRKSRTTEYLKRCLYTVQIGSNDYLNNYFMPLNYKT 184
           GQ  S   Q+ N+  T+S++  LLG ++R  +YLKRC+Y+V +GSNDYLNNYFMP  Y +
Sbjct: 132 GQRISFSGQVRNYQTTVSQVVQLLGDETRAADYLKRCIYSVGLGSNDYLNNYFMPTFYSS 191

Query: 185 SFQFTPEHFATTLNKELTRQLKTLYEQGARKVAVFGVGSIGCTPYARANFEHKETPCVDK 244
           S QFTPE +A  L    + QL  LY  GARK A+ G+G++GC+P A A      T CVD+
Sbjct: 192 SRQFTPEQYANDLISRYSTQLNALYNYGARKFALSGIGAVGCSPNALAGSPDGRT-CVDR 251

Query: 245 VNSAVQFFNIGLKSLVEDLNTQLPDAKFIFVD---VFQISTLQPSNIGKMVLDEPCCEV- 304
           +NSA Q FN  L+SLV+ LN   PDAKFI+++   +FQ     P+  G  V +  CC + 
Sbjct: 252 INSANQIFNNKLRSLVDQLNNNHPDAKFIYINAYGIFQDMITNPARFGFRVTNAGCCGIG 311

Query: 305 -LPGQLQCSPFGKVCGDRTQYLFWDGVHPTEIGFTTVASRAFNSQQPGDTHPFDIKHL 358
              GQ+ C P  + C DR  Y+FWD  HPTE     +A R++N+Q   D +P DI  L
Sbjct: 312 RNAGQITCLPGQRPCRDRNAYVFWDAFHPTEAANVIIARRSYNAQSASDAYPMDISRL 360

BLAST of Lag0005732 vs. TAIR 10
Match: AT4G18970.1 (GDSL-like Lipase/Acylhydrolase superfamily protein )

HSP 1 Score: 312.0 bits (798), Expect = 6.3e-85
Identity = 152/342 (44.44%), Postives = 207/342 (60.53%), Query Frame = 0

Query: 23  GKSIVPCYFVFGDSQADNGNNNILNIGMARADYKPYGIDFSKQQTPTGRFTNGRNVPDFI 82
           G  I PCYF+FGDS  D+GNNN L   +ARA+Y PYGIDF  Q  PTGRF+NG+   D I
Sbjct: 22  GDPIAPCYFIFGDSLVDSGNNNRLT-SLARANYFPYGIDF--QYGPTGRFSNGKTTVDVI 81

Query: 83  AKFLGFVGYIPPFENTKGSDILKGVNYASGGSGILPETGRLLGQISSLGKQLENHNHTIS 142
            + LGF  YI P+   +G DIL+GVNYAS  +GI  ETGR LG   +   Q+ NH +T+S
Sbjct: 82  TELLGFDDYITPYSEARGEDILRGVNYASAAAGIREETGRQLGARITFAGQVANHVNTVS 141

Query: 143 KIQALLGRKSRTTEYLKRCLYTVQIGSNDYLNNYFMPLNYKTSFQFTPEHFATTLNKELT 202
           ++  +LG ++    YL +C+Y++ +GSNDYLNNYFMP+ Y T  Q++P+ +A  L    T
Sbjct: 142 QVVNILGDENEAANYLSKCIYSIGLGSNDYLNNYFMPVYYSTGSQYSPDAYANDLINRYT 201

Query: 203 RQLKTLYEQGARKVAVFGVGSIGCTPYARANFEHKETPCVDKVNSAVQFFNIGLKSLVED 262
            QL+ +Y  GARK A+ G+G+IGC+P   A        C +++NSA + FN  L SLV+ 
Sbjct: 202 EQLRIMYNNGARKFALVGIGAIGCSPNELAQNSRDGVTCDERINSANRIFNSKLVSLVDH 261

Query: 263 LNTQLPDAKFIFVD---VFQISTLQPSNIGKMVLDEPCCEV--LPGQLQCSPFGKVCGDR 322
            N   P AKF +++   +FQ     PS  G  V +  CC V    GQ+ C P    C +R
Sbjct: 262 FNQNTPGAKFTYINAYGIFQDMVANPSRYGFRVTNAGCCGVGRNNGQITCLPGQAPCLNR 321

Query: 323 TQYLFWDGVHPTEIGFTTVASRAFNSQQPGDTHPFDIKHLLR 360
            +Y+FWD  HP E     + SR+F  +   D HP+DI+ L R
Sbjct: 322 DEYVFWDAFHPGEAANVVIGSRSFQRESASDAHPYDIQQLAR 360

BLAST of Lag0005732 vs. TAIR 10
Match: AT4G18970.2 (GDSL-like Lipase/Acylhydrolase superfamily protein )

HSP 1 Score: 312.0 bits (798), Expect = 6.3e-85
Identity = 152/342 (44.44%), Postives = 207/342 (60.53%), Query Frame = 0

Query: 23  GKSIVPCYFVFGDSQADNGNNNILNIGMARADYKPYGIDFSKQQTPTGRFTNGRNVPDFI 82
           G  I PCYF+FGDS  D+GNNN L   +ARA+Y PYGIDF  Q  PTGRF+NG+   D I
Sbjct: 22  GDPIAPCYFIFGDSLVDSGNNNRLT-SLARANYFPYGIDF--QYGPTGRFSNGKTTVDVI 81

Query: 83  AKFLGFVGYIPPFENTKGSDILKGVNYASGGSGILPETGRLLGQISSLGKQLENHNHTIS 142
            + LGF  YI P+   +G DIL+GVNYAS  +GI  ETGR LG   +   Q+ NH +T+S
Sbjct: 82  TELLGFDDYITPYSEARGEDILRGVNYASAAAGIREETGRQLGARITFAGQVANHVNTVS 141

Query: 143 KIQALLGRKSRTTEYLKRCLYTVQIGSNDYLNNYFMPLNYKTSFQFTPEHFATTLNKELT 202
           ++  +LG ++    YL +C+Y++ +GSNDYLNNYFMP+ Y T  Q++P+ +A  L    T
Sbjct: 142 QVVNILGDENEAANYLSKCIYSIGLGSNDYLNNYFMPVYYSTGSQYSPDAYANDLINRYT 201

Query: 203 RQLKTLYEQGARKVAVFGVGSIGCTPYARANFEHKETPCVDKVNSAVQFFNIGLKSLVED 262
            QL+ +Y  GARK A+ G+G+IGC+P   A        C +++NSA + FN  L SLV+ 
Sbjct: 202 EQLRIMYNNGARKFALVGIGAIGCSPNELAQNSRDGVTCDERINSANRIFNSKLVSLVDH 261

Query: 263 LNTQLPDAKFIFVD---VFQISTLQPSNIGKMVLDEPCCEV--LPGQLQCSPFGKVCGDR 322
            N   P AKF +++   +FQ     PS  G  V +  CC V    GQ+ C P    C +R
Sbjct: 262 FNQNTPGAKFTYINAYGIFQDMVANPSRYGFRVTNAGCCGVGRNNGQITCLPGQAPCLNR 321

Query: 323 TQYLFWDGVHPTEIGFTTVASRAFNSQQPGDTHPFDIKHLLR 360
            +Y+FWD  HP E     + SR+F  +   D HP+DI+ L R
Sbjct: 322 DEYVFWDAFHPGEAANVVIGSRSFQRESASDAHPYDIQQLAR 360

BLAST of Lag0005732 vs. TAIR 10
Match: AT1G29660.1 (GDSL-like Lipase/Acylhydrolase superfamily protein )

HSP 1 Score: 309.7 bits (792), Expect = 3.1e-84
Identity = 156/336 (46.43%), Postives = 206/336 (61.31%), Query Frame = 0

Query: 27  VPCYFVFGDSQADNGNNNILNIGMARADYKPYGIDFSKQQTPTGRFTNGRNVPDFIAKFL 86
           VPCYF+FGDS  DNGNNN L   +ARADY PYGIDF     PTGRF+NGR   D + + L
Sbjct: 30  VPCYFIFGDSLVDNGNNNRLR-SIARADYFPYGIDFG---GPTGRFSNGRTTVDVLTELL 89

Query: 87  GFVGYIPPFENTKGSDILKGVNYASGGSGILPETGRLLGQISSLGKQLENHNHTISKIQA 146
           GF  YIP +    G +IL+GVNYAS  +GI  ETG  LGQ  +   Q+EN+ +T++++  
Sbjct: 90  GFDNYIPAYSTVSGQEILQGVNYASAAAGIREETGAQLGQRITFSGQVENYKNTVAQVVE 149

Query: 147 LLGRKSRTTEYLKRCLYTVQIGSNDYLNNYFMPLNYKTSFQFTPEHFATTLNKELTRQLK 206
           +LG +    +YLKRC+Y+V +GSNDYLNNYFMP  Y TS Q+TPE +A  L      QL 
Sbjct: 150 ILGDEYTAADYLKRCIYSVGMGSNDYLNNYFMPQFYSTSRQYTPEQYADDLISRYRDQLN 209

Query: 207 TLYEQGARKVAVFGVGSIGCTPYARANFEHKETPCVDKVNSAVQFFNIGLKSLVEDLNTQ 266
            LY  GARK A+ G+G+IGC+P A A      T CV+++NSA + FN  L S+V+ LN  
Sbjct: 210 ALYNYGARKFALVGIGAIGCSPNALAQGSQDGTTCVERINSANRIFNNRLISMVQQLNNA 269

Query: 267 LPDAKFIFVD---VFQISTLQPSNIGKMVLDEPCCEV--LPGQLQCSPFGKVCGDRTQYL 326
             DA F +++    FQ     PS  G    +  CC +    GQL C P    C +R +Y+
Sbjct: 270 HSDASFTYINAYGAFQDIIANPSAYGFTNTNTACCGIGRNGGQLTCLPGEPPCLNRDEYV 329

Query: 327 FWDGVHPTEIGFTTVASRAFNSQQPGDTHPFDIKHL 358
           FWD  HP+    T +A R++N+Q+  D +P DI  L
Sbjct: 330 FWDAFHPSAAANTAIAKRSYNAQRSSDVYPIDISQL 361

BLAST of Lag0005732 vs. TAIR 10
Match: AT5G45670.1 (GDSL-like Lipase/Acylhydrolase superfamily protein )

HSP 1 Score: 307.4 bits (786), Expect = 1.5e-83
Identity = 153/337 (45.40%), Postives = 206/337 (61.13%), Query Frame = 0

Query: 26  IVPCYFVFGDSQADNGNNNILNIGMARADYKPYGIDFSKQQTPTGRFTNGRNVPDFIAKF 85
           I PCYF+FGDS  DNGNNN L   +ARA+Y PYGIDF+    PTGRF+NG    D IA+ 
Sbjct: 26  IAPCYFIFGDSLVDNGNNNQLQ-SLARANYFPYGIDFA--AGPTGRFSNGLTTVDVIAQL 85

Query: 86  LGFVGYIPPFENTKGSDILKGVNYASGGSGILPETGRLLGQISSLGKQLENHNHTISKIQ 145
           LGF  YI P+ + +G DIL+GVNYAS  +GI  ETGR LG   +   Q+ NH +T+S++ 
Sbjct: 86  LGFEDYITPYASARGQDILRGVNYASAAAGIRDETGRQLGGRIAFAGQVANHVNTVSQVV 145

Query: 146 ALLGRKSRTTEYLKRCLYTVQIGSNDYLNNYFMPLNYKTSFQFTPEHFATTLNKELTRQL 205
            +LG ++  + YL +C+Y++ +GSNDYLNNYFMP  Y T  QF+PE +A  L    T QL
Sbjct: 146 NILGDQNEASNYLSKCIYSIGLGSNDYLNNYFMPTFYSTGNQFSPESYADDLVARYTEQL 205

Query: 206 KTLYEQGARKVAVFGVGSIGCTPYARANFEHKETPCVDKVNSAVQFFNIGLKSLVEDLNT 265
           + LY  GARK A+ GVG+IGC+P   A        C +++NSA + FN  L S+V+  N 
Sbjct: 206 RVLYTNGARKFALIGVGAIGCSPNELAQNSRDGRTCDERINSANRIFNSKLISIVDAFNQ 265

Query: 266 QLPDAKFIFVD---VFQISTLQPSNIGKMVLDEPCCEV--LPGQLQCSPFGKVCGDRTQY 325
             PDAKF +++   +FQ     P+  G  V +  CC V    GQ+ C P    C +R +Y
Sbjct: 266 NTPDAKFTYINAYGIFQDIITNPARYGFRVTNAGCCGVGRNNGQITCLPGQAPCLNRNEY 325

Query: 326 LFWDGVHPTEIGFTTVASRAFNSQQPGDTHPFDIKHL 358
           +FWD  HP E     +  R+F  +   D HP+DI+ L
Sbjct: 326 VFWDAFHPGEAANIVIGRRSFKREAASDAHPYDIQQL 359

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
XP_038891276.14.6e-14669.09GDSL esterase/lipase At1g29670-like [Benincasa hispida][more]
XP_011649033.11.1e-13969.05GDSL esterase/lipase At1g29670 [Cucumis sativus] >XP_031737227.1 GDSL esterase/l... [more]
XP_022144504.13.9e-13767.74GDSL esterase/lipase At1g29670-like [Momordica charantia][more]
XP_008441762.12.5e-13667.52PREDICTED: GDSL esterase/lipase At1g29670-like [Cucumis melo] >KAA0051383.1 GDSL... [more]
XP_022973923.13.4e-13362.11GDSL esterase/lipase At1g29670-like [Cucurbita maxima][more]
Match NameE-valueIdentityDescription
Q9C7N41.3e-9048.04GDSL esterase/lipase At1g29670 OS=Arabidopsis thaliana OX=3702 GN=At1g29670 PE=2... [more]
Q93YW88.8e-8444.44GDSL esterase/lipase At4g18970 OS=Arabidopsis thaliana OX=3702 GN=At4g18970 PE=2... [more]
Q9C7N54.4e-8346.43GDSL esterase/lipase At1g29660 OS=Arabidopsis thaliana OX=3702 GN=At1g29660 PE=1... [more]
Q9FK752.2e-8245.40GDSL esterase/lipase At5g45670 OS=Arabidopsis thaliana OX=3702 GN=At5g45670 PE=2... [more]
O644688.9e-7644.85GDSL esterase/lipase At2g19050 OS=Arabidopsis thaliana OX=3702 GN=At2g19050 PE=3... [more]
Match NameE-valueIdentityDescription
A0A0A0LKR65.3e-14069.05Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_2G093840 PE=3 SV=1[more]
A0A6J1CSH41.9e-13767.74GDSL esterase/lipase At1g29670-like OS=Momordica charantia OX=3673 GN=LOC1110141... [more]
A0A5D3DKM81.2e-13667.52GDSL esterase/lipase OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold419... [more]
A0A1S3B4731.2e-13667.52GDSL esterase/lipase At1g29670-like OS=Cucumis melo OX=3656 GN=LOC103485821 PE=3... [more]
A0A6J1IEL61.6e-13362.11GDSL esterase/lipase At1g29670-like OS=Cucurbita maxima OX=3661 GN=LOC111472555 ... [more]
Match NameE-valueIdentityDescription
AT1G29670.19.0e-9248.04GDSL-like Lipase/Acylhydrolase superfamily protein [more]
AT4G18970.16.3e-8544.44GDSL-like Lipase/Acylhydrolase superfamily protein [more]
AT4G18970.26.3e-8544.44GDSL-like Lipase/Acylhydrolase superfamily protein [more]
AT1G29660.13.1e-8446.43GDSL-like Lipase/Acylhydrolase superfamily protein [more]
AT5G45670.11.5e-8345.40GDSL-like Lipase/Acylhydrolase superfamily protein [more]
InterPro
Analysis Name: InterPro Annotations of Sponge gourd (AG-4) v1
Date Performed: 2022-08-01
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR036514SGNH hydrolase superfamilyGENE3D3.40.50.1110SGNH hydrolasecoord: 18..345
e-value: 9.0E-70
score: 237.6
IPR001087GDSL lipase/esterasePFAMPF00657Lipase_GDSLcoord: 30..337
e-value: 1.2E-31
score: 111.0
NoneNo IPR availablePANTHERPTHR45650:SF62SUBFAMILY NOT NAMEDcoord: 7..358
NoneNo IPR availablePANTHERPTHR45650GDSL-LIKE LIPASE/ACYLHYDROLASE-RELATEDcoord: 7..358
NoneNo IPR availableSUPERFAMILY52266SGNH hydrolasecoord: 30..340
IPR035669GDSL lipase/esterase-like, plantCDDcd01837SGNH_plant_lipase_likecoord: 28..342
e-value: 4.78042E-108
score: 317.25

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Lag0005732.1Lag0005732.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
cellular_component GO:0016021 integral component of membrane
molecular_function GO:0016788 hydrolase activity, acting on ester bonds