Lag0005717 (gene) Sponge gourd (AG‐4) v1

Overview


Name	Lag0005717
Type	gene
Organism	Luffa acutangula (Sponge gourd (AG‐4) v1)
Description	Katanin p60 ATPase-containing subunit A1
Location	chr6: 27103476 .. 27105183 (-)
RNA-Seq Expression	Lag0005717
Synteny	Lag0005717

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: polypeptideexonCDS

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGCCGTCGCGGTTTTCGTCCGTAGTTTCGTTGTCTTCGCCGTCGTTCGTCAGTTCTTCGTGGTGTTCACACGCTGTCCGTCAATTCGTGGTCTTCACACGCTCGTTCGTCATCGTCTTCACAGCACCGTGTACGTCAGTTCGTGGTGTTCACACGTCGTTCGTCAGTTCTTCGTGGTGTTCGCACACCCTATGGAGCCACTTGATAGGTCTGCAAAGCGTATTTATATTCCTCTTCCTAATTTTGTGAGTAGGAAGGAACTTATCAGGATAAATCTAAAAACTGTCGAGGTAAATCAAATACCCATTCATTAACTATCAGAATGTTATTGGACTGATTGTACTTGCATAATTATTTTTGTTAATTCTTATCTCCTTAGGAAATTCGAGATGCTTTATTCTTGAACCAATTGCCTCATGTTTTTCTCTCCTCTTCCGTTTGTTTTGTATTTTATTTTTAATTACTTCTATTGGATTGTTTATCATGGTTTGCAAAGAAATCCTTGTCTTAGCCTATAAGAAATATATTTTAGCTCAAATGGTTGTTGATTTGAACTTACCATGCAATAAACTATTTTCTTAGTCTGAATAAATTTGATGTAAACGAGTTATGATTATCCATACTAGGTGGCTCCTGATGTGAATATCGATTATGTTGCTCGCCCGGACAGAAGGATATAGTGGGGACGATCTCACAAATGTCTGCAGATATGCTTCTTTGAATGGAATGAGACGTAAAATAGCCGGAAAAACTCGCGATGAGATTAAGAATATGCCCAAGGATGAGATTTCAAAGGACCCTGTTGCCATGTGCGACTTTGAAGAAGCATTGAAGAAGGTACAGCGAGGTGTTTCTGCTGCTGATATCGAACGACATGAGAAGTGGTTTTCAGAATTTGGGTCTGCATAAATTACACGCTATCATGAAGATCAGTTACGGATTCACCACTTTCCATTCTGCAAAGTGATTTGTTTATTAATTTTGTTTTTATGATAATCATTGTGTTGTCACGGTGTGATTAATAGTAATCCAAGTGTCTGCTGCCTCTTTTACAGCTTGTAAACCTTTGCTTCCTTTAACCACTGGTTCTATGTAACTTTATCAATTACGAAGAAGTTGAAGTTTCGATTGATATCCATTTCACCAATGAAATGTATTATTTTCTTTTTCTATTATGTTGCTGGAAACTCACTCTATAAAATTAGTTTAAACACTTTTCAGAACCTAGCTTCAATTTATAATACTGGTTTATGTTTATTTAGTTTACTTTTTTTTTAAATATTAAGTTTGGGTTTTGTGTGTGTGTATTATACTGGAGGTTGAGCATTATTGGTGACACCACCAACTCTTGTCTTTACCAGAAAAACTCGAAATGGGGGGGGGGGGGATTTCAGTTTTAAATAGTGAACTAGCTCTTTGCAGCCATTTAGCAACAAAAATCATGAAGGAAGGTTTAATTCTATTTTAAATATTCTGATTTGTTTTGTAGGTACTGTTGTGTGCTCAATCAAAAAGTGTGGGCAAGGAACTTGCAACGCATCTAATGCTTCATTTCTTGGTTTCGATTATGAGTGCAATTTTGGTTGGAGGAAACTCCAAATTGGCTCGTTCTCCTTCGCCTATTGTGTTCTTCCCAACTGTAAGAATCTCTCTCTTCTCCTCTCTCGCTGCAAATGCACTCACACTCTCTGTAGATTGTGCTTTATAG

mRNA sequence

ATGCCGTCGCGGTTTTCGTCCGTAGTTTCGTTGTCTTCGCCGTCGTTCGTCAGTTCTTCGTGGTGTTCACACGCTGTCCGTCAATTCGTGGTCTTCACACGCTCGTTCGTCATCGTCTTCACAGCACCGTGTACGTCAGTTCGTGGTGTTCACACGTCGTTCGTCAGTTCTTCGTGGTGTTCGCACACCCTATGGAGCCACTTGATAGAAGGATATAGTGGGGACGATCTCACAAATGTCTGCAGATATGCTTCTTTGAATGGAATGAGACGTAAAATAGCCGGAAAAACTCGCGATGAGATTAAGAATATGCCCAAGGATGAGATTTCAAAGGACCCTGTTGCCATGTGCGACTTTGAAGAAGCATTGAAGAAGGTACTGTTGTGTGCTCAATCAAAAAGTGTGGGCAAGGAACTTGCAACGCATCTAATGCTTCATTTCTTGGTTTCGATTATGAGTGCAATTTTGGTTGGAGGAAACTCCAAATTGGCTCGTTCTCCTTCGCCTATTGTGTTCTTCCCAACTGTAAGAATCTCTCTCTTCTCCTCTCTCGCTGCAAATGCACTCACACTCTCTGTAGATTGTGCTTTATAG

Coding sequence (CDS)

ATGCCGTCGCGGTTTTCGTCCGTAGTTTCGTTGTCTTCGCCGTCGTTCGTCAGTTCTTCGTGGTGTTCACACGCTGTCCGTCAATTCGTGGTCTTCACACGCTCGTTCGTCATCGTCTTCACAGCACCGTGTACGTCAGTTCGTGGTGTTCACACGTCGTTCGTCAGTTCTTCGTGGTGTTCGCACACCCTATGGAGCCACTTGATAGAAGGATATAGTGGGGACGATCTCACAAATGTCTGCAGATATGCTTCTTTGAATGGAATGAGACGTAAAATAGCCGGAAAAACTCGCGATGAGATTAAGAATATGCCCAAGGATGAGATTTCAAAGGACCCTGTTGCCATGTGCGACTTTGAAGAAGCATTGAAGAAGGTACTGTTGTGTGCTCAATCAAAAAGTGTGGGCAAGGAACTTGCAACGCATCTAATGCTTCATTTCTTGGTTTCGATTATGAGTGCAATTTTGGTTGGAGGAAACTCCAAATTGGCTCGTTCTCCTTCGCCTATTGTGTTCTTCCCAACTGTAAGAATCTCTCTCTTCTCCTCTCTCGCTGCAAATGCACTCACACTCTCTGTAGATTGTGCTTTATAG

Protein sequence

MPSRFSSVVSLSSPSFVSSSWCSHAVRQFVVFTRSFVIVFTAPCTSVRGVHTSFVSSSWCSHTLWSHLIEGYSGDDLTNVCRYASLNGMRRKIAGKTRDEIKNMPKDEISKDPVAMCDFEEALKKVLLCAQSKSVGKELATHLMLHFLVSIMSAILVGGNSKLARSPSPIVFFPTVRISLFSSLAANALTLSVDCAL

Homology

[Close]

BLAST of Lag0005717 vs. NCBI nr
Match: XP_022150913.1 (katanin p60 ATPase-containing subunit A1 isoform X2 [Momordica charantia])

HSP 1 Score: 115.9 bits (289), Expect = 3.9e-22
Identity = 56/57 (98.25%), Postives = 56/57 (98.25%), Query Frame = 0


Query: 70  EGYSGDDLTNVCRYASLNGMRRKIAGKTRDEIKNMPKDEISKDPVAMCDFEEALKKV 127
           EGYSGDDLTNVCR ASLNGMRRKIAGKTRDEIKNMPKDEISKDPVAMCDFEEALKKV
Sbjct: 442 EGYSGDDLTNVCRDASLNGMRRKIAGKTRDEIKNMPKDEISKDPVAMCDFEEALKKV 498

[Close]

BLAST of Lag0005717 vs. NCBI nr
Match: XP_022150917.1 (katanin p60 ATPase-containing subunit A1 isoform X3 [Momordica charantia])

HSP 1 Score: 115.9 bits (289), Expect = 3.9e-22
Identity = 56/57 (98.25%), Postives = 56/57 (98.25%), Query Frame = 0


Query: 70  EGYSGDDLTNVCRYASLNGMRRKIAGKTRDEIKNMPKDEISKDPVAMCDFEEALKKV 127
           EGYSGDDLTNVCR ASLNGMRRKIAGKTRDEIKNMPKDEISKDPVAMCDFEEALKKV
Sbjct: 410 EGYSGDDLTNVCRDASLNGMRRKIAGKTRDEIKNMPKDEISKDPVAMCDFEEALKKV 466

[Close]

BLAST of Lag0005717 vs. NCBI nr
Match: KAF1882961.1 (hypothetical protein Lal_00003143 [Lupinus albus])

HSP 1 Score: 115.9 bits (289), Expect = 3.9e-22
Identity = 56/57 (98.25%), Postives = 56/57 (98.25%), Query Frame = 0


Query: 70  EGYSGDDLTNVCRYASLNGMRRKIAGKTRDEIKNMPKDEISKDPVAMCDFEEALKKV 127
           EGYSGDDLTNVCR ASLNGMRRKIAGKTRDEIKNMPKDEISKDPVAMCDFEEALKKV
Sbjct: 477 EGYSGDDLTNVCRDASLNGMRRKIAGKTRDEIKNMPKDEISKDPVAMCDFEEALKKV 533

[Close]

BLAST of Lag0005717 vs. NCBI nr
Match: XP_016171262.1 (katanin p60 ATPase-containing subunit A1 [Arachis ipaensis])

HSP 1 Score: 115.9 bits (289), Expect = 3.9e-22
Identity = 56/57 (98.25%), Postives = 56/57 (98.25%), Query Frame = 0


Query: 70  EGYSGDDLTNVCRYASLNGMRRKIAGKTRDEIKNMPKDEISKDPVAMCDFEEALKKV 127
           EGYSGDDLTNVCR ASLNGMRRKIAGKTRDEIKNMPKDEISKDPVAMCDFEEALKKV
Sbjct: 534 EGYSGDDLTNVCRDASLNGMRRKIAGKTRDEIKNMPKDEISKDPVAMCDFEEALKKV 590

[Close]

BLAST of Lag0005717 vs. NCBI nr
Match: XP_015933613.1 (katanin p60 ATPase-containing subunit A1 [Arachis duranensis] >XP_025611065.1 katanin p60 ATPase-containing subunit A1 [Arachis hypogaea] >XP_025671147.1 katanin p60 ATPase-containing subunit A1 [Arachis hypogaea] >QHN94646.1 Katanin p60 ATPase-containing subunit [Arachis hypogaea] >RYR50756.1 hypothetical protein Ahy_A07g037375 [Arachis hypogaea])

HSP 1 Score: 115.9 bits (289), Expect = 3.9e-22
Identity = 56/57 (98.25%), Postives = 56/57 (98.25%), Query Frame = 0


Query: 70  EGYSGDDLTNVCRYASLNGMRRKIAGKTRDEIKNMPKDEISKDPVAMCDFEEALKKV 127
           EGYSGDDLTNVCR ASLNGMRRKIAGKTRDEIKNMPKDEISKDPVAMCDFEEALKKV
Sbjct: 455 EGYSGDDLTNVCRDASLNGMRRKIAGKTRDEIKNMPKDEISKDPVAMCDFEEALKKV 511

[Close]

BLAST of Lag0005717 vs. ExPASy Swiss-Prot
Match: Q9SEX2 (Katanin p60 ATPase-containing subunit A1 OS=Arabidopsis thaliana OX=3702 GN=AAA1 PE=1 SV=1)

HSP 1 Score: 108.6 bits (270), Expect = 8.1e-23
Identity = 52/68 (76.47%), Postives = 57/68 (83.82%), Query Frame = 0


Query: 70  EGYSGDDLTNVCRYASLNGMRRKIAGKTRDEIKNMPKDEISKDPVAMCDFEEALKKVLLC 129
           EGYSGDDLTNVCR AS+NGMRRKIAGKTRDEIKNM KD+IS DPVAMCDFEEA++KV   
Sbjct: 445 EGYSGDDLTNVCRDASMNGMRRKIAGKTRDEIKNMSKDDISNDPVAMCDFEEAIRKVQPS 504

Query: 130 AQSKSVGK 138
             S  + K
Sbjct: 505 VSSSDIEK 512

[Close]

BLAST of Lag0005717 vs. ExPASy Swiss-Prot
Match: Q5RII9 (Katanin p60 ATPase-containing subunit A1 OS=Danio rerio OX=7955 GN=katna1 PE=2 SV=1)

HSP 1 Score: 72.4 bits (176), Expect = 6.4e-12
Identity = 41/67 (61.19%), Postives = 48/67 (71.64%), Query Frame = 0


Query: 69  IEGYSGDDLTNVCRYASLNGMRRKIAGKTRDEIKNMPKDEISKDPVAMCDFEEALKKVLL 128
           +EGYSG D+TNVCR ASL  MRR+I G T +EI+N+PKDE+   P  M DFE ALKKV  
Sbjct: 407 MEGYSGADITNVCRDASLMAMRRRIEGLTPEEIRNLPKDEMHM-PTTMEDFETALKKV-- 466

Query: 129 CAQSKSV 136
              SKSV
Sbjct: 467 ---SKSV 467

[Close]

BLAST of Lag0005717 vs. ExPASy Swiss-Prot
Match: Q5U3S1 (Katanin p60 ATPase-containing subunit A-like 1 OS=Danio rerio OX=7955 GN=katnal1 PE=2 SV=1)

HSP 1 Score: 68.6 bits (166), Expect = 9.3e-11
Identity = 39/73 (53.42%), Postives = 51/73 (69.86%), Query Frame = 0


Query: 63  TLWSHLIEGYSGDDLTNVCRYASLNGMRRKIAGKTRDEIKNMPKDEISKDPVAMCDFEEA 122
           T+++  IEGYSG D+TNVCR AS+  MRR+I G + +EI+ + KDE+   PV M DFE A
Sbjct: 404 TVFAEKIEGYSGADITNVCRDASMMAMRRRIQGLSPEEIRALSKDELQM-PVTMEDFELA 463

Query: 123 LKKVLLCAQSKSV 136
           LKK+     SKSV
Sbjct: 464 LKKI-----SKSV 470

[Close]

BLAST of Lag0005717 vs. ExPASy Swiss-Prot
Match: Q1HGK7 (Katanin p60 ATPase-containing subunit A1 OS=Gallus gallus OX=9031 GN=KATNA1 PE=2 SV=1)

HSP 1 Score: 67.4 bits (163), Expect = 2.1e-10
Identity = 39/67 (58.21%), Postives = 47/67 (70.15%), Query Frame = 0


Query: 69  IEGYSGDDLTNVCRYASLNGMRRKIAGKTRDEIKNMPKDEISKDPVAMCDFEEALKKVLL 128
           +EGYSG D+TNVCR ASL  MRR+I G T +EI+N+ +DE+   P  M DFE ALKKV  
Sbjct: 414 MEGYSGADITNVCRDASLMAMRRRIEGLTPEEIRNLSRDEMHM-PTTMEDFEIALKKV-- 473

Query: 129 CAQSKSV 136
              SKSV
Sbjct: 474 ---SKSV 474

[Close]

BLAST of Lag0005717 vs. ExPASy Swiss-Prot
Match: O75449 (Katanin p60 ATPase-containing subunit A1 OS=Homo sapiens OX=9606 GN=KATNA1 PE=1 SV=1)

HSP 1 Score: 67.4 bits (163), Expect = 2.1e-10
Identity = 39/67 (58.21%), Postives = 47/67 (70.15%), Query Frame = 0


Query: 69  IEGYSGDDLTNVCRYASLNGMRRKIAGKTRDEIKNMPKDEISKDPVAMCDFEEALKKVLL 128
           +EGYSG D+TNVCR ASL  MRR+I G T +EI+N+ K+E+   P  M DFE ALKKV  
Sbjct: 413 MEGYSGADITNVCRDASLMAMRRRIEGLTPEEIRNLSKEEMHM-PTTMEDFEMALKKV-- 472

Query: 129 CAQSKSV 136
              SKSV
Sbjct: 473 ---SKSV 473

[Close]

BLAST of Lag0005717 vs. ExPASy TrEMBL
Match: A0A6A4MZ94 (Katanin p60 ATPase-containing subunit A1 OS=Lupinus albus OX=3870 GN=KATNA1 PE=3 SV=1)

HSP 1 Score: 115.9 bits (289), Expect = 1.9e-22
Identity = 56/57 (98.25%), Postives = 56/57 (98.25%), Query Frame = 0


Query: 70  EGYSGDDLTNVCRYASLNGMRRKIAGKTRDEIKNMPKDEISKDPVAMCDFEEALKKV 127
           EGYSGDDLTNVCR ASLNGMRRKIAGKTRDEIKNMPKDEISKDPVAMCDFEEALKKV
Sbjct: 458 EGYSGDDLTNVCRDASLNGMRRKIAGKTRDEIKNMPKDEISKDPVAMCDFEEALKKV 514

[Close]

BLAST of Lag0005717 vs. ExPASy TrEMBL
Match: A0A6A5N6N1 (Katanin p60 ATPase-containing subunit A1 OS=Lupinus albus OX=3870 GN=KATNA1 PE=3 SV=1)

HSP 1 Score: 115.9 bits (289), Expect = 1.9e-22
Identity = 56/57 (98.25%), Postives = 56/57 (98.25%), Query Frame = 0


Query: 70  EGYSGDDLTNVCRYASLNGMRRKIAGKTRDEIKNMPKDEISKDPVAMCDFEEALKKV 127
           EGYSGDDLTNVCR ASLNGMRRKIAGKTRDEIKNMPKDEISKDPVAMCDFEEALKKV
Sbjct: 477 EGYSGDDLTNVCRDASLNGMRRKIAGKTRDEIKNMPKDEISKDPVAMCDFEEALKKV 533

[Close]

BLAST of Lag0005717 vs. ExPASy TrEMBL
Match: A0A6J1DC29 (Katanin p60 ATPase-containing subunit A1 OS=Momordica charantia OX=3673 GN=LOC111018943 PE=3 SV=1)

HSP 1 Score: 115.9 bits (289), Expect = 1.9e-22
Identity = 56/57 (98.25%), Postives = 56/57 (98.25%), Query Frame = 0


Query: 70  EGYSGDDLTNVCRYASLNGMRRKIAGKTRDEIKNMPKDEISKDPVAMCDFEEALKKV 127
           EGYSGDDLTNVCR ASLNGMRRKIAGKTRDEIKNMPKDEISKDPVAMCDFEEALKKV
Sbjct: 410 EGYSGDDLTNVCRDASLNGMRRKIAGKTRDEIKNMPKDEISKDPVAMCDFEEALKKV 466

[Close]

BLAST of Lag0005717 vs. ExPASy TrEMBL
Match: A0A6J1DAQ9 (Katanin p60 ATPase-containing subunit A1 OS=Momordica charantia OX=3673 GN=LOC111018943 PE=3 SV=1)

HSP 1 Score: 115.9 bits (289), Expect = 1.9e-22
Identity = 56/57 (98.25%), Postives = 56/57 (98.25%), Query Frame = 0


Query: 70  EGYSGDDLTNVCRYASLNGMRRKIAGKTRDEIKNMPKDEISKDPVAMCDFEEALKKV 127
           EGYSGDDLTNVCR ASLNGMRRKIAGKTRDEIKNMPKDEISKDPVAMCDFEEALKKV
Sbjct: 442 EGYSGDDLTNVCRDASLNGMRRKIAGKTRDEIKNMPKDEISKDPVAMCDFEEALKKV 498

[Close]

BLAST of Lag0005717 vs. ExPASy TrEMBL
Match: A0A6J1DC18 (Katanin p60 ATPase-containing subunit A1 OS=Momordica charantia OX=3673 GN=LOC111018943 PE=3 SV=1)

HSP 1 Score: 115.9 bits (289), Expect = 1.9e-22
Identity = 56/57 (98.25%), Postives = 56/57 (98.25%), Query Frame = 0


Query: 70  EGYSGDDLTNVCRYASLNGMRRKIAGKTRDEIKNMPKDEISKDPVAMCDFEEALKKV 127
           EGYSGDDLTNVCR ASLNGMRRKIAGKTRDEIKNMPKDEISKDPVAMCDFEEALKKV
Sbjct: 445 EGYSGDDLTNVCRDASLNGMRRKIAGKTRDEIKNMPKDEISKDPVAMCDFEEALKKV 501

The following BLAST results are available for this feature:

BLAST of Lag0005717 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_022150913.1	3.9e-22	98.25	katanin p60 ATPase-containing subunit A1 isoform X2 [Momordica charantia]	[more]
XP_022150917.1	3.9e-22	98.25	katanin p60 ATPase-containing subunit A1 isoform X3 [Momordica charantia]	[more]
KAF1882961.1	3.9e-22	98.25	hypothetical protein Lal_00003143 [Lupinus albus]	[more]
XP_016171262.1	3.9e-22	98.25	katanin p60 ATPase-containing subunit A1 [Arachis ipaensis]	[more]
XP_015933613.1	3.9e-22	98.25	katanin p60 ATPase-containing subunit A1 [Arachis duranensis] >XP_025611065.1 ka...	[more]

BLAST of Lag0005717 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
Q9SEX2	8.1e-23	76.47	Katanin p60 ATPase-containing subunit A1 OS=Arabidopsis thaliana OX=3702 GN=AAA1...	[more]
Q5RII9	6.4e-12	61.19	Katanin p60 ATPase-containing subunit A1 OS=Danio rerio OX=7955 GN=katna1 PE=2 S...	[more]
Q5U3S1	9.3e-11	53.42	Katanin p60 ATPase-containing subunit A-like 1 OS=Danio rerio OX=7955 GN=katnal1...	[more]
Q1HGK7	2.1e-10	58.21	Katanin p60 ATPase-containing subunit A1 OS=Gallus gallus OX=9031 GN=KATNA1 PE=2...	[more]
O75449	2.1e-10	58.21	Katanin p60 ATPase-containing subunit A1 OS=Homo sapiens OX=9606 GN=KATNA1 PE=1 ...	[more]

BLAST of Lag0005717 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A6A4MZ94	1.9e-22	98.25	Katanin p60 ATPase-containing subunit A1 OS=Lupinus albus OX=3870 GN=KATNA1 PE=3...	[more]
A0A6A5N6N1	1.9e-22	98.25	Katanin p60 ATPase-containing subunit A1 OS=Lupinus albus OX=3870 GN=KATNA1 PE=3...	[more]
A0A6J1DC29	1.9e-22	98.25	Katanin p60 ATPase-containing subunit A1 OS=Momordica charantia OX=3673 GN=LOC11...	[more]
A0A6J1DAQ9	1.9e-22	98.25	Katanin p60 ATPase-containing subunit A1 OS=Momordica charantia OX=3673 GN=LOC11...	[more]
A0A6J1DC18	1.9e-22	98.25	Katanin p60 ATPase-containing subunit A1 OS=Momordica charantia OX=3673 GN=LOC11...	[more]

InterPro

Analysis Name: InterPro Annotations of Sponge gourd (AG-4) v1
Date Performed: 2022-08-01


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
None	No IPR available	GENE3D	1.10.8.60		coord: 55..124 e-value: 1.3E-10 score: 43.3
None	No IPR available	PANTHER	PTHR23074:SF156	KATANIN P60 ATPASE-CONTAINING SUBUNIT A1	coord: 69..136
None	No IPR available	PANTHER	PTHR23074	AAA DOMAIN-CONTAINING	coord: 69..136

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
Lag0005717.1	Lag0005717.1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0051013	microtubule severing
cellular_component	GO:0005737	cytoplasm
cellular_component	GO:0005874	microtubule
molecular_function	GO:0005524	ATP binding
molecular_function	GO:0016887	ATP hydrolysis activity
molecular_function	GO:0016853	isomerase activity
molecular_function	GO:0008017	microtubule binding
molecular_function	GO:0008568	microtubule-severing ATPase activity