Lag0005655 (gene) Sponge gourd (AG‐4) v1

Overview


Name	Lag0005655
Type	gene
Organism	Luffa acutangula (Sponge gourd (AG‐4) v1)
Description	Ankyrin repeat-containing protein
Location	chr6: 25237945 .. 25238514 (-)
RNA-Seq Expression	Lag0005655
Synteny	Lag0005655

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: polypeptideexonCDS

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGGCTTACGAGAAAGACCGAAATGGCATGTCGGCCCTGCACATTGCAGCGGAGGAAGGCCATCTTGGCGTCTTGGAAACCATTGTCGGACTCTGTCCAGACAGCTGCGAGCTGTTGGATGTTAAAGGCAGAACAGCCCTCCATGTCGCTGTCGCTAAGAGGAAGGTCAATGCTGTCAGGACGATGTTGAAAATGCCAAACTTTATGAAGATGGTGAACCAGCAGGACATAGATGGAAACACGGCTTTGCATCTCGCTGCCATTAATGGCCACATTTATCTAATCAGGATGTTGGCTGCCAGTGGAAGGGTTGCTAAAAATATTAACAACAACCAAGGATTTACTACCAATGACATTATTCGATTGAGTCCCACGTTTAATTCCTTAGGAAAGGTATGCCTTTTATCTCTATATCTCTGCCTATCTACACATATTAAGGTTGAGTTATATATATTTGTATGTTGTTAACACATTTAACATGAGGACTCTTACATTTTACAACAACTAGTACATAAAATTAAGGCCATGTTTGGGGTTGATTGTAAAAAGTGATTGTAGATAAATTTTTGA

mRNA sequence

ATGGCTTACGAGAAAGACCGAAATGGCATGTCGGCCCTGCACATTGCAGCGGAGGAAGGCCATCTTGGCGTCTTGGAAACCATTGTCGGACTCTGTCCAGACAGCTGCGAGCTGTTGGATGTTAAAGGCAGAACAGCCCTCCATGTCGCTGTCGCTAAGAGGAAGGTCAATGCTGTCAGGACGATGTTGAAAATGCCAAACTTTATGAAGATGGTGAACCAGCAGGACATAGATGGAAACACGGCTTTGCATCTCGCTGCCATTAATGGCCACATTTATCTAATCAGGATGTTGGCTGCCAGTGGAAGGGTTGCTAAAAATATTAACAACAACCAAGGATTTACTACCAATGACATTATTCGATTGAGTCCCACGTTTAATTCCTTAGGAAAGATAAATTTTTGA

Coding sequence (CDS)

ATGGCTTACGAGAAAGACCGAAATGGCATGTCGGCCCTGCACATTGCAGCGGAGGAAGGCCATCTTGGCGTCTTGGAAACCATTGTCGGACTCTGTCCAGACAGCTGCGAGCTGTTGGATGTTAAAGGCAGAACAGCCCTCCATGTCGCTGTCGCTAAGAGGAAGGTCAATGCTGTCAGGACGATGTTGAAAATGCCAAACTTTATGAAGATGGTGAACCAGCAGGACATAGATGGAAACACGGCTTTGCATCTCGCTGCCATTAATGGCCACATTTATCTAATCAGGATGTTGGCTGCCAGTGGAAGGGTTGCTAAAAATATTAACAACAACCAAGGATTTACTACCAATGACATTATTCGATTGAGTCCCACGTTTAATTCCTTAGGAAAGATAAATTTTTGA

Protein sequence

MAYEKDRNGMSALHIAAEEGHLGVLETIVGLCPDSCELLDVKGRTALHVAVAKRKVNAVRTMLKMPNFMKMVNQQDIDGNTALHLAAINGHIYLIRMLAASGRVAKNINNNQGFTTNDIIRLSPTFNSLGKINF

Homology

[Close]

BLAST of Lag0005655 vs. NCBI nr
Match: XP_008456252.1 (PREDICTED: protein ACCELERATED CELL DEATH 6-like [Cucumis melo])

HSP 1 Score: 161.8 bits (408), Expect = 4.2e-36
Identity = 81/127 (63.78%), Postives = 98/127 (77.17%), Query Frame = 0


Query: 1   MAYEKDRNGMSALHIAAEEGHLGVLETIVGLCPDSCELLDVKGRTALHVAVAKRKVNAVR 60
           MAYEKD+NG+SALH+AA+EG   VL+T   LCPDSCELLD K +T LHVAVA R+  AVR
Sbjct: 310 MAYEKDKNGVSALHLAAKEGRSAVLKTFARLCPDSCELLDSKDQTVLHVAVANRQAYAVR 369

Query: 61  TMLKMPNFMKMVNQQDIDGNTALHLAAINGHIYLIRMLAASGRVAKNINNNQGFTTNDII 120
            ML++ +F  +VNQ+DIDGNT LH+AAI G   +I MLA+ GRV K I N  GFTTNDII
Sbjct: 370 KMLELRSFRNLVNQKDIDGNTPLHVAAIVGDYVIIMMLASHGRVDKKIMNQAGFTTNDII 429

Query: 121 RLSPTFN 128
           RL+P F+
Sbjct: 430 RLNPKFS 436

[Close]

BLAST of Lag0005655 vs. NCBI nr
Match: KAA0063587.1 (protein ACCELERATED CELL DEATH 6-like [Cucumis melo var. makuwa] >TYK18440.1 protein ACCELERATED CELL DEATH 6-like [Cucumis melo var. makuwa])

HSP 1 Score: 161.8 bits (408), Expect = 4.2e-36
Identity = 81/127 (63.78%), Postives = 98/127 (77.17%), Query Frame = 0


Query: 1   MAYEKDRNGMSALHIAAEEGHLGVLETIVGLCPDSCELLDVKGRTALHVAVAKRKVNAVR 60
           MAYEKD+NG+SALH+AA+EG   VL+T   LCPDSCELLD K +T LHVAVA R+  AVR
Sbjct: 310 MAYEKDKNGVSALHLAAKEGRSAVLKTFARLCPDSCELLDSKDQTVLHVAVANRQAYAVR 369

Query: 61  TMLKMPNFMKMVNQQDIDGNTALHLAAINGHIYLIRMLAASGRVAKNINNNQGFTTNDII 120
            ML++ +F  +VNQ+DIDGNT LH+AAI G   +I MLA+ GRV K I N  GFTTNDII
Sbjct: 370 KMLELRSFRNLVNQKDIDGNTPLHVAAIVGDYVIIMMLASHGRVDKKIMNKAGFTTNDII 429

Query: 121 RLSPTFN 128
           RL+P F+
Sbjct: 430 RLNPKFS 436

[Close]

BLAST of Lag0005655 vs. NCBI nr
Match: XP_008456239.1 (PREDICTED: protein ACCELERATED CELL DEATH 6-like [Cucumis melo])

HSP 1 Score: 152.9 bits (385), Expect = 1.9e-33
Identity = 78/127 (61.42%), Postives = 94/127 (74.02%), Query Frame = 0


Query: 1   MAYEKDRNGMSALHIAAEEGHLGVLETIVGLCPDSCELLDVKGRTALHVAVAKRKVNAVR 60
           MAY KD NG+SALH+AA+EG + VL+T   LCPDSCEL D++ RTALH AVA  +  AVR
Sbjct: 314 MAYTKDNNGISALHLAAKEGCISVLKTFTKLCPDSCELTDLRDRTALHFAVANHQAYAVR 373

Query: 61  TMLKMPNFMKMVNQQDIDGNTALHLAAINGHIYLIRMLAASGRVAKNINNNQGFTTNDII 120
            ML+  +F  +VNQ+DIDGNT LHLAAI G   ++ MLAA+ RV K I NN GFTTNDII
Sbjct: 374 KMLEFGSFRNLVNQRDIDGNTPLHLAAIVGDFVIVMMLAANERVDKKIMNNAGFTTNDII 433

Query: 121 RLSPTFN 128
           R S  F+
Sbjct: 434 RSSLKFS 440

[Close]

BLAST of Lag0005655 vs. NCBI nr
Match: KAA0063588.1 (protein ACCELERATED CELL DEATH 6-like [Cucumis melo var. makuwa] >TYK18439.1 protein ACCELERATED CELL DEATH 6-like [Cucumis melo var. makuwa])

HSP 1 Score: 152.9 bits (385), Expect = 1.9e-33
Identity = 78/127 (61.42%), Postives = 94/127 (74.02%), Query Frame = 0


Query: 1   MAYEKDRNGMSALHIAAEEGHLGVLETIVGLCPDSCELLDVKGRTALHVAVAKRKVNAVR 60
           MAY KD NG+SALH+AA+EG + VL+T   LCPDSCEL D++ RTALH AVA  +  AVR
Sbjct: 298 MAYTKDNNGISALHLAAKEGCISVLKTFTKLCPDSCELTDLRDRTALHFAVANHQAYAVR 357

Query: 61  TMLKMPNFMKMVNQQDIDGNTALHLAAINGHIYLIRMLAASGRVAKNINNNQGFTTNDII 120
            ML+  +F  +VNQ+DIDGNT LHLAAI G   ++ MLAA+ RV K I NN GFTTNDII
Sbjct: 358 KMLEFGSFRNLVNQRDIDGNTPLHLAAIVGDFVIVMMLAANERVDKKIMNNAGFTTNDII 417

Query: 121 RLSPTFN 128
           R S  F+
Sbjct: 418 RSSLKFS 424

[Close]

BLAST of Lag0005655 vs. NCBI nr
Match: XP_038877254.1 (LOW QUALITY PROTEIN: ankyrin repeat-containing protein NPR4-like [Benincasa hispida])

HSP 1 Score: 152.1 bits (383), Expect = 3.3e-33
Identity = 76/127 (59.84%), Postives = 95/127 (74.80%), Query Frame = 0


Query: 1   MAYEKDRNGMSALHIAAEEGHLGVLETIVGLCPDSCELLDVKGRTALHVAVAKRKVNAVR 60
           MAY+KD  G+ ALH+AA+EG   VL+T   +CPDSCELLD   +TALHVAVA R+  AVR
Sbjct: 324 MAYKKDNKGVPALHLAAKEGRSAVLKTFAKVCPDSCELLDPNDQTALHVAVANRQAYAVR 383

Query: 61  TMLKMPNFMKMVNQQDIDGNTALHLAAINGHIYLIRMLAASGRVAKNINNNQGFTTNDII 120
            ML++ +F  +VNQ+DIDGNT LH+AAI G   ++ MLAA+GRV K I N  GFTTNDII
Sbjct: 384 KMLELGSFRNLVNQKDIDGNTPLHVAAIVGDYVIVMMLAANGRVDKKIMNKAGFTTNDII 443

Query: 121 RLSPTFN 128
           RL+  F+
Sbjct: 444 RLNSKFS 450

[Close]

BLAST of Lag0005655 vs. ExPASy Swiss-Prot
Match: Q8GYH5 (Ankyrin repeat-containing protein BDA1 OS=Arabidopsis thaliana OX=3702 GN=BAD1 PE=1 SV=1)

HSP 1 Score: 72.4 bits (176), Expect = 4.4e-12
Identity = 39/125 (31.20%), Postives = 69/125 (55.20%), Query Frame = 0


Query: 5   KDRNGMSALHIAAEEGHLGVLETIVGLCPDSCELLDVKGRTALHVAVAKRKVNAVRT--- 64
           + R GM+ LH+ A++G + +L   +  CP+S + ++V G T LH+ +   K   ++    
Sbjct: 101 RGRGGMTPLHLVAKKGDVDLLTDFLLACPESIKDVNVNGETILHITIMNDKYEQLKVLTG 160

Query: 65  -MLKMPN----FMKMVNQQDIDGNTALHLAAINGHIYLIRMLAASGRVAKNINNNQGFTT 122
            M KM +    F+ ++N++D  GNT LHLAA   +  +++ L     + +NI N  G T 
Sbjct: 161 WMQKMRDSDDVFIDVLNRRDRGGNTVLHLAAYENNDKVVKQLVKCLSLDRNIQNKSGMTA 220

[Close]

BLAST of Lag0005655 vs. ExPASy Swiss-Prot
Match: Q8LPS2 (Protein ACCELERATED CELL DEATH 6 OS=Arabidopsis thaliana OX=3702 GN=ACD6 PE=1 SV=1)

HSP 1 Score: 61.6 bits (148), Expect = 7.7e-09
Identity = 40/118 (33.90%), Postives = 57/118 (48.31%), Query Frame = 0


Query: 3   YEKDRNGMSALHIAAEEGHLGVLETIVGLCPDSCELLDVKGRTALHVAVAKRKVNAVRTM 62
           Y  D++G   +H AA+  H  +++  +  CP S  LL+  G+  LHVA AK + +    M
Sbjct: 324 YVCDQDGSFPIHSAAKNEHYEIIKEFIKRCPASKYLLNRLGQNILHVA-AKNEASLTAYM 383

Query: 63  LKMPNFMKMVN-QQDIDGNTALHLAAINGHIYLIRMLAASGRVAKNINNNQGFTTNDI 120
           L      K +   QD+DGNT LHLA +N     I  LA+       + N  G    DI
Sbjct: 384 LMHDKDTKHLGVGQDVDGNTPLHLAVMNWDFDSITCLASRNHEILKLRNKSGLRARDI 440

[Close]

BLAST of Lag0005655 vs. ExPASy Swiss-Prot
Match: Q01484 (Ankyrin-2 OS=Homo sapiens OX=9606 GN=ANK2 PE=1 SV=4)

HSP 1 Score: 61.6 bits (148), Expect = 7.7e-09
Identity = 36/110 (32.73%), Postives = 64/110 (58.18%), Query Frame = 0


Query: 6   DRNGMSALHIAAEEGHLGVLETIVGLCPDSCELLDVKGRTALHVAVAKRKVNAVRTMLKM 65
           ++NG++ALH+AA+EGH+G+++ ++G    S +    KG TALH+A    +   V+ ++K 
Sbjct: 61  NQNGLNALHLAAKEGHVGLVQELLGR-GSSVDSATKKGNTALHIASLAGQAEVVKVLVKE 120

Query: 66  PNFMKMVNQQDIDGNTALHLAAINGHIYLIRMLAASGRVAKNINNNQGFT 116
                 +N Q  +G T L++AA   HI +++ L  +G   ++     GFT
Sbjct: 121 G---ANINAQSQNGFTPLYMAAQENHIDVVKYLLENG-ANQSTATEDGFT 165

[Close]

BLAST of Lag0005655 vs. ExPASy Swiss-Prot
Match: Q8C8R3 (Ankyrin-2 OS=Mus musculus OX=10090 GN=Ank2 PE=1 SV=2)

HSP 1 Score: 61.6 bits (148), Expect = 7.7e-09
Identity = 36/110 (32.73%), Postives = 64/110 (58.18%), Query Frame = 0


Query: 6   DRNGMSALHIAAEEGHLGVLETIVGLCPDSCELLDVKGRTALHVAVAKRKVNAVRTMLKM 65
           ++NG++ALH+AA+EGH+G+++ ++G    S +    KG TALH+A    +   V+ ++K 
Sbjct: 61  NQNGLNALHLAAKEGHVGLVQELLGR-GSSVDSATKKGNTALHIASLAGQAEVVKVLVKE 120

Query: 66  PNFMKMVNQQDIDGNTALHLAAINGHIYLIRMLAASGRVAKNINNNQGFT 116
                 +N Q  +G T L++AA   HI +++ L  +G   ++     GFT
Sbjct: 121 G---ANINAQSQNGFTPLYMAAQENHIDVVKYLLENG-ANQSTATEDGFT 165

[Close]

BLAST of Lag0005655 vs. ExPASy Swiss-Prot
Match: Q9ZU96 (Ankyrin repeat-containing protein At2g01680 OS=Arabidopsis thaliana OX=3702 GN=At2g01680 PE=2 SV=1)

HSP 1 Score: 57.4 bits (137), Expect = 1.5e-07
Identity = 28/111 (25.23%), Postives = 58/111 (52.25%), Query Frame = 0


Query: 5   KDRNGMSALHIAAEEGHLGVLETIVGLCPDSCELLDVKGRTALHVAVAKRKVNAVRTMLK 64
           + ++ M+A H+AA+ GHLG+++ ++ L P+ C + D    + L+ A  +  +  V  ML 
Sbjct: 90  RSKSDMNAFHVAAKRGHLGIVKELLRLWPELCRICDASNTSPLYAAAVQDHLEIVNAMLD 149

Query: 65  MPNFMKMVNQQDIDGNTALHLAAINGHIYLIRMLAASGRVAKNINNNQGFT 116
           +     M+ ++  +G T+LH A   G + +++ L         + + +G T
Sbjct: 150 VDPSCAMIVRK--NGKTSLHTAGRYGLLRIVKALIEKDAAIVGVKDKKGQT 198

The following BLAST results are available for this feature:

BLAST of Lag0005655 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_008456252.1	4.2e-36	63.78	PREDICTED: protein ACCELERATED CELL DEATH 6-like [Cucumis melo]	[more]
KAA0063587.1	4.2e-36	63.78	protein ACCELERATED CELL DEATH 6-like [Cucumis melo var. makuwa] >TYK18440.1 pro...	[more]
XP_008456239.1	1.9e-33	61.42	PREDICTED: protein ACCELERATED CELL DEATH 6-like [Cucumis melo]	[more]
KAA0063588.1	1.9e-33	61.42	protein ACCELERATED CELL DEATH 6-like [Cucumis melo var. makuwa] >TYK18439.1 pro...	[more]
XP_038877254.1	3.3e-33	59.84	LOW QUALITY PROTEIN: ankyrin repeat-containing protein NPR4-like [Benincasa hisp...	[more]

BLAST of Lag0005655 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
Q8GYH5	4.4e-12	31.20	Ankyrin repeat-containing protein BDA1 OS=Arabidopsis thaliana OX=3702 GN=BAD1 P...	[more]
Q8LPS2	7.7e-09	33.90	Protein ACCELERATED CELL DEATH 6 OS=Arabidopsis thaliana OX=3702 GN=ACD6 PE=1 SV...	[more]
Q01484	7.7e-09	32.73	Ankyrin-2 OS=Homo sapiens OX=9606 GN=ANK2 PE=1 SV=4	[more]
Q8C8R3	7.7e-09	32.73	Ankyrin-2 OS=Mus musculus OX=10090 GN=Ank2 PE=1 SV=2	[more]
Q9ZU96	1.5e-07	25.23	Ankyrin repeat-containing protein At2g01680 OS=Arabidopsis thaliana OX=3702 GN=A...	[more]

InterPro

Analysis Name: InterPro Annotations of Sponge gourd (AG-4) v1
Date Performed: 2022-08-01


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR002110	Ankyrin repeat	SMART	SM00248	ANK_2a	coord: 78..110 e-value: 2.5E-4 score: 30.4 coord: 8..38 e-value: 0.044 score: 22.9 coord: 42..71 e-value: 7.8 score: 15.4
IPR002110	Ankyrin repeat	PROSITE	PS50088	ANK_REPEAT	coord: 8..29 score: 8.76305
IPR002110	Ankyrin repeat	PROSITE	PS50088	ANK_REPEAT	coord: 78..110 score: 10.579329
IPR036770	Ankyrin repeat-containing domain superfamily	GENE3D	1.25.40.20		coord: 2..129 e-value: 1.2E-23 score: 85.5
IPR036770	Ankyrin repeat-containing domain superfamily	SUPERFAMILY	48403	Ankyrin repeat	coord: 4..120
IPR020683	Ankyrin repeat-containing domain	PFAM	PF12796	Ank_2	coord: 40..102 e-value: 2.1E-10 score: 41.1
None	No IPR available	PANTHER	PTHR24186:SF30	ANKYRIN REPEAT-CONTAINING PROTEIN ITN1-LIKE	coord: 2..125
None	No IPR available	PANTHER	PTHR24186	PROTEIN PHOSPHATASE 1 REGULATORY SUBUNIT	coord: 2..125
None	No IPR available	PROSITE	PS50297	ANK_REP_REGION	coord: 78..102 score: 10.418106
None	No IPR available	PROSITE	PS50297	ANK_REP_REGION	coord: 8..29 score: 8.74663

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
Lag0005655.1	Lag0005655.1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
cellular_component	GO:0016020	membrane
molecular_function	GO:0005515	protein binding