Lag0005654 (gene) Sponge gourd (AG‐4) v1

Overview
NameLag0005654
Typegene
OrganismLuffa acutangula (Sponge gourd (AG‐4) v1)
DescriptionAnkyrin repeat family protein
Locationchr6: 25195286 .. 25195726 (-)
RNA-Seq ExpressionLag0005654
SyntenyLag0005654
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonCDSpolypeptide
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGTGGAGCAGATTGTCGAATGCGAACATGGTGGTGGCGGCGCTCATCGCGACAGTAACCTTCAGTGCGGCCTTTCAGGTTCCCGGTGGATACGACAACAACGGCCTGGCGGTGCTACGAAAAAAAGAATCTTTCGGCCGCTACTTGATATTGGATGCTGTATCCTTTTGGTTAGCAGTGGCTTCCATGTCCCTCACCTTCTTAACACATTTTTTCCGTACGACCAAATTTGCTCCGAGTGACTTGGTGTTGTACTTGACGGGTTGGTCTCTTTTTTTCATGGTATTTGCATTCTATGAAGGGATAACTTTGGTCATAAAGGATGGGGAACGTTCACGCTTCTCAACACTGTTGGGTCTTCTGTCTGATATTTACATTCCACTTATATTGCTTATAGGCTTTATCTTACCTTTATGGGGCTCCTGGGATTTGCAAGAATGA

mRNA sequence

ATGTGGAGCAGATTGTCGAATGCGAACATGGTGGTGGCGGCGCTCATCGCGACAGTAACCTTCAGTGCGGCCTTTCAGGTTCCCGGTGGATACGACAACAACGGCCTGGCGGTGCTACGAAAAAAAGAATCTTTCGGCCGCTACTTGATATTGGATGCTGTATCCTTTTGGTTAGCAGTGGCTTCCATGTCCCTCACCTTCTTAACACATTTTTTCCGTACGACCAAATTTGCTCCGAGTGACTTGGTGTTGTACTTGACGGGTTGGTCTCTTTTTTTCATGGTATTTGCATTCTATGAAGGGATAACTTTGGTCATAAAGGATGGGGAACGTTCACGCTTCTCAACACTGTTGGGTCTTCTGTCTGATATTTACATTCCACTTATATTGCTTATAGGCTTTATCTTACCTTTATGGGGCTCCTGGGATTTGCAAGAATGA

Coding sequence (CDS)

ATGTGGAGCAGATTGTCGAATGCGAACATGGTGGTGGCGGCGCTCATCGCGACAGTAACCTTCAGTGCGGCCTTTCAGGTTCCCGGTGGATACGACAACAACGGCCTGGCGGTGCTACGAAAAAAAGAATCTTTCGGCCGCTACTTGATATTGGATGCTGTATCCTTTTGGTTAGCAGTGGCTTCCATGTCCCTCACCTTCTTAACACATTTTTTCCGTACGACCAAATTTGCTCCGAGTGACTTGGTGTTGTACTTGACGGGTTGGTCTCTTTTTTTCATGGTATTTGCATTCTATGAAGGGATAACTTTGGTCATAAAGGATGGGGAACGTTCACGCTTCTCAACACTGTTGGGTCTTCTGTCTGATATTTACATTCCACTTATATTGCTTATAGGCTTTATCTTACCTTTATGGGGCTCCTGGGATTTGCAAGAATGA

Protein sequence

MWSRLSNANMVVAALIATVTFSAAFQVPGGYDNNGLAVLRKKESFGRYLILDAVSFWLAVASMSLTFLTHFFRTTKFAPSDLVLYLTGWSLFFMVFAFYEGITLVIKDGERSRFSTLLGLLSDIYIPLILLIGFILPLWGSWDLQE
Homology
BLAST of Lag0005654 vs. NCBI nr
Match: KAA0063588.1 (protein ACCELERATED CELL DEATH 6-like [Cucumis melo var. makuwa] >TYK18439.1 protein ACCELERATED CELL DEATH 6-like [Cucumis melo var. makuwa])

HSP 1 Score: 113.2 bits (282), Expect = 1.9e-21
Identity = 64/140 (45.71%), Postives = 96/140 (68.57%), Query Frame = 0

Query: 1   MWSRLSNANMVVAALIATVTFSAAFQVPGGYDNNGLAVLRKKESFGRYLILDAVSFWLAV 60
           +WS++S+AN+VVA +IATVTFSAAFQVPGGY+NNG+AVLR+ + F  Y++ DA+SF  A 
Sbjct: 492 IWSQVSDANLVVATIIATVTFSAAFQVPGGYNNNGIAVLRRAKQFRLYMLYDALSFGFAA 551

Query: 61  ASMSLTFLTHFFRT-TKFA-PSDLVLYLTGWSLFFMVFAFYEGITLVI----KDGERSRF 120
           ASM +TF T  F   + F+ P   + +LTG S++FMVFAF  G +L +    K G  +R+
Sbjct: 552 ASMFITFFTGLFGVGSGFSYPRRWMTFLTGLSVWFMVFAFMMGTSLAVDEHSKRGWVARY 611

Query: 121 STLLGLLSDIYIPLILLIGF 135
              +  +S +++  +L + +
Sbjct: 612 VPCISFISPVFLLGVLAVNW 631

BLAST of Lag0005654 vs. NCBI nr
Match: XP_008456239.1 (PREDICTED: protein ACCELERATED CELL DEATH 6-like [Cucumis melo])

HSP 1 Score: 112.8 bits (281), Expect = 2.4e-21
Identity = 64/140 (45.71%), Postives = 96/140 (68.57%), Query Frame = 0

Query: 1   MWSRLSNANMVVAALIATVTFSAAFQVPGGYDNNGLAVLRKKESFGRYLILDAVSFWLAV 60
           +WS++S+AN+VVA +IATVTFSAAFQVPGGY+NNG+AVLR+ + F  Y++ DA+SF  A 
Sbjct: 508 IWSQVSDANLVVATIIATVTFSAAFQVPGGYNNNGIAVLRRAKQFRLYMLYDALSFGFAA 567

Query: 61  ASMSLTFLTHFFRT-TKFA-PSDLVLYLTGWSLFFMVFAFYEGITLVI----KDGERSRF 120
           ASM +TF T  F   + F+ P   + +LTG S++FMVFAF  G +L +    K G  +R+
Sbjct: 568 ASMFITFFTGLFGVGSGFSYPRRWMTFLTGSSVWFMVFAFMMGTSLAVDEHSKRGWVARY 627

Query: 121 STLLGLLSDIYIPLILLIGF 135
              +  +S +++  +L + +
Sbjct: 628 VPCISFISPVFLLGVLAVNW 647

BLAST of Lag0005654 vs. NCBI nr
Match: KAG7030321.1 (Protein ACCELERATED CELL DEATH 6, partial [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 109.8 bits (273), Expect = 2.1e-20
Identity = 63/140 (45.00%), Postives = 90/140 (64.29%), Query Frame = 0

Query: 1   MWSRLSNANMVVAALIATVTFSAAFQVPGGYDNNGLAVLRKKESFGRYLILDAVSFWLAV 60
           +WS++S+AN+VVA +IATVTFSAAFQVPGGY+NNG+A LR+ + F  Y+  DA+SF  A 
Sbjct: 463 IWSQVSDANLVVATIIATVTFSAAFQVPGGYNNNGIATLRRAKQFRLYMTFDALSFGFAA 522

Query: 61  ASMSLTFLTHFFRTTK--FAPSDLVLYLTGWSLFFMVFAFYEGITLVI----KDGERSRF 120
           ASM +TF T  F        P   V +LTG SL+FMVFAF  G ++ +    K G ++R 
Sbjct: 523 ASMFVTFFTGLFGVDAGFTYPRKWVTFLTGLSLWFMVFAFMMGTSVAMDEHSKLGGQARV 582

Query: 121 STLLGLLSDIYIPLILLIGF 135
              +  +S ++   +L + +
Sbjct: 583 LPCISFISPVFFLGVLAVNW 602

BLAST of Lag0005654 vs. NCBI nr
Match: XP_004139385.1 (protein ACCELERATED CELL DEATH 6 [Cucumis sativus] >KGN60785.1 hypothetical protein Csa_019478 [Cucumis sativus])

HSP 1 Score: 109.0 bits (271), Expect = 3.5e-20
Identity = 64/119 (53.78%), Postives = 82/119 (68.91%), Query Frame = 0

Query: 1   MWSRLSNANMVVAALIATVTFSAAFQVPGGYDNNGLAVLRKKESFGRYLILDAVSFWLAV 60
           +WS LS+AN+VVA +IATVTFSAAFQVPGGY ++G+AVLRK++ F  YL+ DA+SF  A 
Sbjct: 505 IWSELSDANLVVATIIATVTFSAAFQVPGGYQSDGMAVLRKEKYFRLYLLSDALSFGFAA 564

Query: 61  ASMSLTFLTHFFRTTK--FAPSDLVLYLTGWSLFFMVFAFYEGITLVIKDGERSRFSTL 118
           ASM +TF T  F        P   V +LTG S++FMVFAF  G + V+   E S F+ L
Sbjct: 565 ASMFVTFFTGLFGANSGFSYPRRWVTFLTGTSVWFMVFAFMLGTSAVM--AEHSGFAGL 621

BLAST of Lag0005654 vs. NCBI nr
Match: KAG6599329.1 (Ankyrin repeat-containing protein ITN1, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 107.8 bits (268), Expect = 7.8e-20
Identity = 58/110 (52.73%), Postives = 77/110 (70.00%), Query Frame = 0

Query: 1   MWSRLSNANMVVAALIATVTFSAAFQVPGGYDNNGLAVLRKKESFGRYLILDAVSFWLAV 60
           +WS++S+AN+VVA +IATVTFSAAFQVPGGY+NNG+A LR+ + F  Y+  DA+SF  A 
Sbjct: 429 IWSQVSDANLVVATIIATVTFSAAFQVPGGYNNNGIATLRRAKQFRLYMTFDALSFGFAA 488

Query: 61  ASMSLTFLTHFFRTTK--FAPSDLVLYLTGWSLFFMVFAFYEGITLVIKD 109
           ASM +TF T  F        P   V +LTG SL+FMVFAF  G ++ + +
Sbjct: 489 ASMFVTFFTGLFGVDAGFTYPRKWVTFLTGLSLWFMVFAFMMGTSVAMDE 538

BLAST of Lag0005654 vs. ExPASy Swiss-Prot
Match: A2CIR5 (Ankyrin repeat-containing protein NPR4 OS=Oryza sativa subsp. japonica OX=39947 GN=NPR4 PE=2 SV=1)

HSP 1 Score: 49.7 bits (117), Expect = 3.3e-05
Identity = 25/56 (44.64%), Postives = 38/56 (67.86%), Query Frame = 0

Query: 6   SNANMVVAALIATVTFSAAFQVPGGYDNNGLAVLRKKESFGRYLILDAVSFWLAVA 62
           +N+  VVA L ATV F+A F VPGG  NNG+AV+ +  SF  + I +A++ + ++A
Sbjct: 411 TNSVTVVAVLFATVAFAAIFTVPGGNANNGVAVVVQAASFRIFFIFNAIALFTSLA 466

BLAST of Lag0005654 vs. ExPASy Swiss-Prot
Match: Q9C7A2 (Ankyrin repeat-containing protein ITN1 OS=Arabidopsis thaliana OX=3702 GN=ITN1 PE=1 SV=1)

HSP 1 Score: 45.8 bits (107), Expect = 4.8e-04
Identity = 27/65 (41.54%), Postives = 42/65 (64.62%), Query Frame = 0

Query: 6   SNANMVVAALIATVTFSAAFQVPGGYDNNGLAVLRKKESFGRYLILDAVSFW--LAVASM 65
           +N+  VVA L ATV F+A F VPGG +N+G AV+  + SF  + I +A++ +  LAV  +
Sbjct: 419 TNSVTVVAVLFATVAFAAIFTVPGGDNNDGSAVVVGRASFKIFFIFNALALFTSLAVVVV 478

Query: 66  SLTFL 69
            +T +
Sbjct: 479 QITLV 483

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
KAA0063588.11.9e-2145.71protein ACCELERATED CELL DEATH 6-like [Cucumis melo var. makuwa] >TYK18439.1 pro... [more]
XP_008456239.12.4e-2145.71PREDICTED: protein ACCELERATED CELL DEATH 6-like [Cucumis melo][more]
KAG7030321.12.1e-2045.00Protein ACCELERATED CELL DEATH 6, partial [Cucurbita argyrosperma subsp. argyros... [more]
XP_004139385.13.5e-2053.78protein ACCELERATED CELL DEATH 6 [Cucumis sativus] >KGN60785.1 hypothetical prot... [more]
KAG6599329.17.8e-2052.73Ankyrin repeat-containing protein ITN1, partial [Cucurbita argyrosperma subsp. s... [more]
Match NameE-valueIdentityDescription
A2CIR53.3e-0544.64Ankyrin repeat-containing protein NPR4 OS=Oryza sativa subsp. japonica OX=39947 ... [more]
Q9C7A24.8e-0441.54Ankyrin repeat-containing protein ITN1 OS=Arabidopsis thaliana OX=3702 GN=ITN1 P... [more]
InterPro
Analysis Name: InterPro Annotations of Sponge gourd (AG-4) v1
Date Performed: 2022-08-01
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR026961PGG domainPFAMPF13962PGGcoord: 5..104
e-value: 2.0E-19
score: 69.5
NoneNo IPR availablePANTHERPTHR24186PROTEIN PHOSPHATASE 1 REGULATORY SUBUNITcoord: 5..119
NoneNo IPR availablePANTHERPTHR24186:SF30ANKYRIN REPEAT-CONTAINING PROTEIN ITN1-LIKEcoord: 5..119

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Lag0005654.1Lag0005654.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
cellular_component GO:0016021 integral component of membrane