Lag0005510 (gene) Sponge gourd (AG‐4) v1

BLAST of Lag0005510 vs. NCBI nr
Match: KYP50444.1 (Retrovirus-related Pol polyprotein from transposon TNT 1-94 [Cajanus cajan])

HSP 1 Score: 185.3 bits (469), Expect = 9.4e-43
Identity = 146/392 (37.24%), Postives = 199/392 (50.77%), Query Frame = 0

Query: 1   MKSHVDNLGQAGSPVSTRSLISQVLLGLDEEFNPVIAMIQGRGGITWSEMQAELLVYEKR 60
           MK   D+L  AGS VST  L++Q L GLD E+NP++  +  +  +TW EMQA+LL YE R
Sbjct: 58  MKEIADDLALAGSSVSTMDLVTQTLAGLDNEYNPIVVQLSDKEHLTWVEMQAQLLTYENR 117

Query: 61  LELQNTFKSSLMLSQGPSVNMANVKDNNNQKNRGNNYNRQSGCGRGNQRGGGGRGRSKGR 120
           LE  N  +S+L L+  PS N++ +  N   K+         G GRG Q   G RG   GR
Sbjct: 118 LEQINN-QSNLTLN--PSSNISTILYNRRGKSNA------FGGGRGGQINRGARG---GR 177

Query: 121 GYGSFNNGKPTCQ---------------FN-----LHNSVQPSAFMANQNYNSYNLFVAS 180
           G G     +  CQ               FN      ++  Q S     QNYN +N +VAS
Sbjct: 178 GRGRATKDRIVCQVCCKPGHAASHCYHRFNKNYIGQNSDEQKSEKDKEQNYN-FNAYVAS 237

Query: 181 SETVVDPNWYADSGASNHVTVDYTNLTNPVDYEGKERVTVGDGNQLQITCVGSSSLNAGR 240
             TV D +WY DSGASNHVT D   +    + +GK  +TVG+G  L+I   G SSL+  +
Sbjct: 238 PSTVEDLDWYFDSGASNHVTYDQNKVQEVNENDGKSFLTVGNGANLKIIACGDSSLDTQQ 297

Query: 241 QS-------YGQANAERVLSEGLYRFDDG------RTAAVEKSKSVICSSGTLSRKNNVD 300
           +S       Y     + +LS     FD+         A   K K     +G +  +  + 
Sbjct: 298 KSLNLKDILYVPKITKNLLSISKLTFDNDIYVEFHDVACFVKDK----LTGRILLEGKIK 357

Query: 301 LSVLTL---SSSVN----VVVS-KVTWHRRVGHPSEKVFDLIVKQCNLPYRLNEKSTFCE 352
             +  L   S+S N    V  S K TWHR++GHP+ KV + ++K CN+     E   FCE
Sbjct: 358 DGLYQLPGGSTSTNKRPHVFFSIKETWHRKLGHPNSKVLNEVMKLCNIEASPCENFEFCE 417

BLAST of Lag0005510 vs. NCBI nr
Match: PNX76291.1 (gag/pol polyprotein - maize retrotransposon Hopscotch, partial [Trifolium pratense])

HSP 1 Score: 184.1 bits (466), Expect = 2.1e-42
Identity = 127/378 (33.60%), Postives = 190/378 (50.26%), Query Frame = 0

Query: 1   MKSHVDNLGQAGSPVSTRSLISQVLLGLDEEFNPVIAMIQGRGGITWSEMQAELLVYEKR 60
           MK+  D L  AG+P+ST  LI Q L GLD E+NPV+  +  +  ++W ++QA+LL +E R
Sbjct: 145 MKNLADKLKLAGNPISTSDLIIQTLNGLDSEYNPVVVKLSDQTTLSWVDLQAQLLTFENR 204

Query: 61  LELQNTFKSSLMLSQGPSVNMANVKDN-NNQKNRGNNYNRQSGCGRGNQRGGGGRGRSKG 120
           +E  N+  +   L+   + N+A   D+  N+ N  NN+   +   RG+   G   GR +G
Sbjct: 205 IEQLNSLTN---LTLNATANVAKKSDHRGNRFNSNNNWRGSNNNWRGSNFRGWRGGRGRG 264

Query: 121 RGYGSFNNGKPTCQF-NLHNSVQPSAFMA-------------NQNYNSYNLFVASSETVV 180
           R +      K TCQ   L N +    F               N    S+N F+AS  ++ 
Sbjct: 265 RSF------KTTCQVCGLDNHIAIDCFYRFDKTYSRSNHSANNDKQGSHNAFLASQNSIE 324

Query: 181 DPNWYADSGASNHVTVDYTNLTNPVDYEGKERVTVGDGNQLQITCVGSS---SLNAGRQS 240
           D +WY DSGASNHVT       N  ++ GK  + VG+G +L+I   GSS   SLN     
Sbjct: 325 DYDWYFDSGASNHVTHQTDKFQNLSEHHGKNSLIVGNGEKLEIVATGSSKLKSLNLHDIL 384

Query: 241 YGQANAERVLSEGLYRFDDGRTAAVEKS----KSVICSSGTLSRKNNVDLSVLTLSSSVN 300
           Y     + +LS      D+      +++    K  +     L       L  L+   S  
Sbjct: 385 YVPKITKNLLSVSKLAADNNILVEFDENCCFVKDKLTGKAILRGILKDGLYQLSEKDSSA 444

Query: 301 VVVSKVTWHRRVGHPSEKVFDLIVKQCNLPYRLNEKSTFCEAFQFGKAHALPFPHSVSRA 357
            V  K +WHR++GHP+ KV D+++K CN+    +++ +FCEA Q+GK H LPF  S S A
Sbjct: 445 YVSIKESWHRKLGHPNNKVLDIVLKSCNVKLSPSDQFSFCEACQYGKMHFLPFKTSFSHA 504

BLAST of Lag0005510 vs. NCBI nr
Match: XP_022151683.1 (uncharacterized protein LOC111019598 [Momordica charantia])

HSP 1 Score: 181.8 bits (460), Expect = 1.0e-41
Identity = 132/338 (39.05%), Postives = 181/338 (53.55%), Query Frame = 0

Query: 1   MKSHVDNLGQAGSPVSTRSLISQVLLGLDEEFNPVIAMIQGRGGITWSEMQAELLVYEKR 60
           MKSH DNL  AGS VS R L+SQVL GLDEE+NP++  +QG+  ++WSEM AELL YEKR
Sbjct: 175 MKSHADNLALAGSSVSVRDLVSQVLTGLDEEYNPIVVAVQGKVNLSWSEMHAELLTYEKR 234

Query: 61  LELQNTFKSSLML--SQGPSVN-------MANVKDNNNQKNRGNNYNRQSGCGRGNQRGG 120
           LE QN+ KS + +  +Q PSVN         N + NN   + G+N +R    G G QRG 
Sbjct: 235 LEYQNSLKSGIPINQTQTPSVNYVDGRSFQTNQRTNNGNNSHGSNTHR----GGGYQRGS 294

Query: 121 GGRGRSKGRGYGSFNNGKPTCQFNLHNSVQ-PSAFMANQNYNSYNLFVASSETVVDPNWY 180
            G+ R++GRG       +PT   N   S   P+ F A+    +    V + ETV+DP+WY
Sbjct: 295 FGQ-RNRGRG------PQPTQHKNFTPSNSGPNVFAAHHTSTT----VTTPETVIDPSWY 354

Query: 181 ADSGASNHVTVDYTNLTNPVDYEGKERVTVGDGNQLQITCVGSSSLNAGRQSY------- 240
           ADSGA++HVT +  N+   VDY G E V V +GN+L I+ +GS++++A   S        
Sbjct: 355 ADSGATSHVTANPNNVEQKVDYSGTENVIVANGNKLSISHIGSTNIHASGGSLKLKDVLR 414

Query: 241 ------------GQANAERVLSEGLYRFDDGR---------TAAVEKSKSVICSSGTLSR 300
                       G+   +  L + LYR D            TA +     V  S+ TLS 
Sbjct: 415 VPDIAKNLDKASGRTLLKGTLKDNLYRLDRSHRSPPATPTLTAPLFAHTVVSLSNNTLSS 474

BLAST of Lag0005510 vs. NCBI nr
Match: PNX81106.1 (glutamate receptor [Trifolium pratense])

HSP 1 Score: 176.0 bits (445), Expect = 5.7e-40
Identity = 127/378 (33.60%), Postives = 180/378 (47.62%), Query Frame = 0

Query: 1   MKSHVDNLGQAGSPVSTRSLISQVLLGLDEEFNPVIAMIQGRGGITWSEMQAELLVYEKR 60
           MK   D L  AGS +S   L+ Q L GLD ++NPV+  +  +  ++W E+QA+LL +E R
Sbjct: 146 MKGLADKLKLAGSAISNSDLVIQTLNGLDMDYNPVVVKLSDQIDLSWVELQAQLLAFESR 205

Query: 61  LELQNTFKSSLMLSQGPSVNMANVKDNNNQKNRGNNYNRQSGCGRGNQRGGGGRGRSKGR 120
           LE  N   +   L+   + N+A     N  K  GN +N      RGN RG   RG   GR
Sbjct: 206 LEQLNNINN---LNLNATANVA-----NKTKFSGNRFN-----SRGNWRGSNFRGMRGGR 265

Query: 121 GYGSFNNGKPTCQFNLHNSVQPSAF--------------MANQNYNSYNLFVASSETVVD 180
           G G F+N KP CQ    +    + F                N+   ++N FVAS     D
Sbjct: 266 GKGRFHNSKPVCQVCTGSHTAINCFYRFDKSYTGPNYSNSENEKQGTHNAFVASPYNGQD 325

Query: 181 PNWYADSGASNHVTVDYTNLTNPVDYEGKERVTVGDGNQLQITCVGSSSLNA---GRQSY 240
             WY DSGASNHVT       +  ++ GK  + VG+G +L+I   GSS +N        Y
Sbjct: 326 YEWYFDSGASNHVTHQADRFEDLTEHTGKNSLMVGNGEKLKIVASGSSKINTVNLHNVLY 385

Query: 241 GQANAERVLS--------EGLYRFDDGRTAAVEKSKSVICSSGTLSRKNNVDLSVLTLSS 300
                + +LS          +  FD       +K    +   G L +     LS  T ++
Sbjct: 386 VPQITKNLLSVSKLTSDNNAIVEFDHDYCYVKDKVTGKVLLKGRL-KDGLYQLSDATQTN 445

Query: 301 SVNVVVSKV--TWHRRVGHPSEKVFDLIVKQCNLPYRLNEKSTFCEAFQFGKAHALPFPH 352
             + V   V  +WHR++GHP+ K  D ++K CN+    +++  FCEA Q GK+H LPF  
Sbjct: 446 KDSCVYLSVKESWHRKLGHPNNKALDRVLKICNVKTSPSDQLEFCEACQLGKSHLLPFKS 505

BLAST of Lag0005510 vs. NCBI nr
Match: GAU19483.1 (hypothetical protein TSUD_77270 [Trifolium subterraneum])

HSP 1 Score: 172.2 bits (435), Expect = 8.2e-39
Identity = 126/363 (34.71%), Postives = 183/363 (50.41%), Query Frame = 0

Query: 1   MKSHVDNLGQAGSPVSTRSLISQVLLGLDEEFNPVIAMIQGRGGITWSEMQAELLVYEKR 60
           MK+ VD L  AG+PVST  LI Q L GLD E+NPV+  +  +  ++W ++QA+LL +E R
Sbjct: 145 MKNLVDKLKLAGNPVSTSDLIIQTLNGLDSEYNPVVVKLSDQTTLSWVDLQAQLLTFESR 204

Query: 61  LELQNTFKSSLMLSQGPSVNMANVKDNNNQKNRGNNYNRQSGCGRGNQRGGGGRGRSKGR 120
           +E  N   +   L+   + N+AN  D+   K+  NN+   +  G    RG G  G++  +
Sbjct: 205 IEQLNNLTN---LTLNATANVANRSDHRG-KSSNNNWRGSNSRGWRGGRGRGKSGKNPCQ 264

Query: 121 GYGSFNNGKPTCQFNLHNSVQPSAFMA-NQNYNSYNLFVASSETVVDPNWYADSGASNHV 180
             G  N+    C      +   S   A +    S+N F+AS  +V D +WY DSGASNHV
Sbjct: 265 VCGLSNHIAIDCFHRFDKTYSRSNHSAGHDKQGSHNAFLASQNSVEDYDWYFDSGASNHV 324

Query: 181 TVDYTNLTNPVDYEGKERVTVGDGNQLQITCVGSS---SLNAGRQSYGQANAERVLSEG- 240
           T       +  ++ GK  + VG+G +L I   GSS   SLN     Y     + +LS   
Sbjct: 325 THQTEKFQDLTEHHGKNSLVVGNGEKLAILATGSSKLKSLNLHDILYVPNITKNLLSVSK 384

Query: 241 -------LYRFDDGRTAAVEKSKSVICSSGTLSRKNNVDLSVLTLSSSVNVVVSKVTWHR 300
                  L  FD+      +K    +   G L +     LS    + S  V V K +WHR
Sbjct: 385 LAADNNILVEFDENCCFVKDKLTGKVILKGLL-KDGLYQLSGTKRNPSAFVSV-KESWHR 444

Query: 301 RVGHPSEKVFDLIVKQCNLPYRLNEKSTFCEAFQFGKAHALPFPHSVSRASSVFELIHAD 352
           R+GHP+ KV D +++ C +    ++  +FCEA Q+GK H LPF  S S A    EL+H D
Sbjct: 445 RLGHPNNKVLDKVLESCKVKVPPSDNFSFCEACQYGKMHLLPFKSSSSHAQEPLELVHTD 501

BLAST of Lag0005510 vs. ExPASy Swiss-Prot
Match: Q9ZT94 (Retrovirus-related Pol polyprotein from transposon RE2 OS=Arabidopsis thaliana OX=3702 GN=RE2 PE=4 SV=1)

HSP 1 Score: 80.1 bits (196), Expect = 5.6e-14
Identity = 97/381 (25.46%), Postives = 162/381 (42.52%), Query Frame = 0

Query: 6   DNLGQAGSPVSTRSLISQVLLGLDEEFNPVIAMIQGRG-GITWSEMQAELLVYEKRLELQ 65
           D L   G P+     + +VL  L +++ PVI  I  +    + +E+   L+  E +L   
Sbjct: 135 DQLALLGKPMDHDEQVERVLENLPDDYKPVIDQIAAKDTPPSLTEIHERLINRESKLLAL 194

Query: 66  NTFKSSLMLSQGPSVNMANVKDNNNQKNRGNNYNRQSGCGRGNQRGGGGRG-RSKGRGYG 125
           N+  + ++      V   N   N NQ NRG+N N  +   R N       G RS  R   
Sbjct: 195 NS--AEVVPITANVVTHRNTNTNRNQNNRGDNRNYNNNNNRSNSWQPSSSGSRSDNRQPK 254

Query: 126 SFNNGKPTCQFNLHNSVQ-------PSAFMANQNYNSYNLFVASSETVVDP-----NWYA 185
            +      C    H++ +        S     Q+ + +  +   +   V+      NW  
Sbjct: 255 PYLGRCQICSVQGHSAKRCPQLHQFQSTTNQQQSTSPFTPWQPRANLAVNSPYNANNWLL 314

Query: 186 DSGASNHVTVDYTNLTNPVDYEGKERVTVGDGNQLQITCVGSSSLNAGRQS-------YG 245
           DSGA++H+T D+ NL+    Y G + V + DG+ + IT  GS+SL    +S       Y 
Sbjct: 315 DSGATHHITSDFNNLSFHQPYTGGDDVMIADGSTIPITHTGSASLPTSSRSLDLNKVLYV 374

Query: 246 QANAERVLSEGLYRFDDGRTAAVE------KSKSVICSSGTLSRKNNVDLSVLTLSSSVN 305
               + ++S  +YR  +    +VE      + K +      L  K   +L    ++SS  
Sbjct: 375 PNIHKNLIS--VYRLCNTNRVSVEFFPASFQVKDLNTGVPLLQGKTKDELYEWPIASSQA 434

Query: 306 VVV-----SKVT---WHRRVGHPSEKVFDLIVKQCNLP-YRLNEKSTFCEAFQFGKAHAL 351
           V +     SK T   WH R+GHPS  + + ++   +LP    + K   C      K+H +
Sbjct: 435 VSMFASPCSKATHSSWHSRLGHPSLAILNSVISNHSLPVLNPSHKLLSCSDCFINKSHKV 494

BLAST of Lag0005510 vs. ExPASy Swiss-Prot
Match: Q94HW2 (Retrovirus-related Pol polyprotein from transposon RE1 OS=Arabidopsis thaliana OX=3702 GN=RE1 PE=2 SV=1)

HSP 1 Score: 74.7 bits (182), Expect = 2.3e-12
Identity = 98/389 (25.19%), Postives = 160/389 (41.13%), Query Frame = 0

Query: 6   DNLGQAGSPVSTRSLISQVLLGLDEEFNPVIAMIQGRG-GITWSEMQAELLVYEKRLELQ 65
           D L   G P+     + +VL  L EE+ PVI  I  +    T +E+   LL +E ++   
Sbjct: 154 DQLALLGKPMDHDEQVERVLENLPEEYKPVIDQIAAKDTPPTLTEIHERLLNHESKILAV 213

Query: 66  NTFKSSLMLSQGPSVNMANVKDNNNQKNRGNNYNRQSGCGRGNQRGGGGRGRSKGRGYGS 125
           ++     + +   S       +NNN  NR N Y+      R N        +S    + +
Sbjct: 214 SSATVIPITANAVSHRNTTTTNNNNNGNRNNRYD-----NRNNNNNSKPWQQSSTNFHPN 273

Query: 126 FNNGKP---TCQF-------------------NLHNSVQPSAFMANQNYNSYNLFVASSE 185
            N  KP    CQ                    ++++   PS F   Q     NL + S  
Sbjct: 274 NNQSKPYLGKCQICGVQGHSAKRCSQLQHFLSSVNSQQPPSPFTPWQ--PRANLALGSPY 333

Query: 186 TVVDPNWYADSGASNHVTVDYTNLTNPVDYEGKERVTVGDGNQLQITCVGSSSLNAGRQS 245
           +    NW  DSGA++H+T D+ NL+    Y G + V V DG+ + I+  GS+SL+   + 
Sbjct: 334 S--SNNWLLDSGATHHITSDFNNLSLHQPYTGGDDVMVADGSTIPISHTGSTSLSTKSRP 393

Query: 246 YGQANAERVLS-----EGLYRFDDGRTAAVE------KSKSVICSSGTLSRKNNVDLSVL 305
               N   V +       +YR  +    +VE      + K +      L  K   +L   
Sbjct: 394 LNLHNILYVPNIHKNLISVYRLCNANGVSVEFFPASFQVKDLNTGVPLLQGKTKDELYEW 453

Query: 306 TLSSSVNVVV-----SKVT---WHRRVGHPSEKVFDLIVKQCNLPYRLNEKSTF--CEAF 351
            ++SS  V +     SK T   WH R+GHP+  + + ++   +L   LN    F  C   
Sbjct: 454 PIASSQPVSLFASPSSKATHSSWHARLGHPAPSILNSVISNYSLSV-LNPSHKFLSCSDC 513

BLAST of Lag0005510 vs. ExPASy Swiss-Prot
Match: P93293 (Uncharacterized mitochondrial protein AtMg00300 OS=Arabidopsis thaliana OX=3702 GN=AtMg00300 PE=4 SV=1)

HSP 1 Score: 50.1 bits (118), Expect = 6.2e-05
Identity = 21/66 (31.82%), Postives = 33/66 (50.00%), Query Frame = 0

Query: 286 WHRRVGHPSEKVFDLIVKQCNLPYRLNEKSTFCEAFQFGKAHALPFPHSVSRASSVFELI 345
           WH R+ H S++  +L+VK+  L         FCE   +GK H + F        +  + +
Sbjct: 72  WHSRLAHMSQRGMELLVKKGFLDSSKVSSLKFCEDCIYGKTHRVNFSTGQHTTKNPLDYV 131

Query: 346 HADLWG 352
           H+DLWG
Sbjct: 132 HSDLWG 137

BLAST of Lag0005510 vs. ExPASy TrEMBL
Match: A0A151S6M8 (Retrovirus-related Pol polyprotein from transposon TNT 1-94 OS=Cajanus cajan OX=3821 GN=KK1_027809 PE=4 SV=1)

HSP 1 Score: 185.3 bits (469), Expect = 4.6e-43
Identity = 146/392 (37.24%), Postives = 199/392 (50.77%), Query Frame = 0

Query: 1   MKSHVDNLGQAGSPVSTRSLISQVLLGLDEEFNPVIAMIQGRGGITWSEMQAELLVYEKR 60
           MK   D+L  AGS VST  L++Q L GLD E+NP++  +  +  +TW EMQA+LL YE R
Sbjct: 58  MKEIADDLALAGSSVSTMDLVTQTLAGLDNEYNPIVVQLSDKEHLTWVEMQAQLLTYENR 117

Query: 61  LELQNTFKSSLMLSQGPSVNMANVKDNNNQKNRGNNYNRQSGCGRGNQRGGGGRGRSKGR 120
           LE  N  +S+L L+  PS N++ +  N   K+         G GRG Q   G RG   GR
Sbjct: 118 LEQINN-QSNLTLN--PSSNISTILYNRRGKSNA------FGGGRGGQINRGARG---GR 177

Query: 121 GYGSFNNGKPTCQ---------------FN-----LHNSVQPSAFMANQNYNSYNLFVAS 180
           G G     +  CQ               FN      ++  Q S     QNYN +N +VAS
Sbjct: 178 GRGRATKDRIVCQVCCKPGHAASHCYHRFNKNYIGQNSDEQKSEKDKEQNYN-FNAYVAS 237

Query: 181 SETVVDPNWYADSGASNHVTVDYTNLTNPVDYEGKERVTVGDGNQLQITCVGSSSLNAGR 240
             TV D +WY DSGASNHVT D   +    + +GK  +TVG+G  L+I   G SSL+  +
Sbjct: 238 PSTVEDLDWYFDSGASNHVTYDQNKVQEVNENDGKSFLTVGNGANLKIIACGDSSLDTQQ 297

Query: 241 QS-------YGQANAERVLSEGLYRFDDG------RTAAVEKSKSVICSSGTLSRKNNVD 300
           +S       Y     + +LS     FD+         A   K K     +G +  +  + 
Sbjct: 298 KSLNLKDILYVPKITKNLLSISKLTFDNDIYVEFHDVACFVKDK----LTGRILLEGKIK 357

Query: 301 LSVLTL---SSSVN----VVVS-KVTWHRRVGHPSEKVFDLIVKQCNLPYRLNEKSTFCE 352
             +  L   S+S N    V  S K TWHR++GHP+ KV + ++K CN+     E   FCE
Sbjct: 358 DGLYQLPGGSTSTNKRPHVFFSIKETWHRKLGHPNSKVLNEVMKLCNIEASPCENFEFCE 417

BLAST of Lag0005510 vs. ExPASy TrEMBL
Match: A0A2K3LCM1 (Gag/pol polyprotein-maize retrotransposon Hopscotch (Fragment) OS=Trifolium pratense OX=57577 GN=L195_g032236 PE=4 SV=1)

HSP 1 Score: 184.1 bits (466), Expect = 1.0e-42
Identity = 127/378 (33.60%), Postives = 190/378 (50.26%), Query Frame = 0

Query: 1   MKSHVDNLGQAGSPVSTRSLISQVLLGLDEEFNPVIAMIQGRGGITWSEMQAELLVYEKR 60
           MK+  D L  AG+P+ST  LI Q L GLD E+NPV+  +  +  ++W ++QA+LL +E R
Sbjct: 145 MKNLADKLKLAGNPISTSDLIIQTLNGLDSEYNPVVVKLSDQTTLSWVDLQAQLLTFENR 204

Query: 61  LELQNTFKSSLMLSQGPSVNMANVKDN-NNQKNRGNNYNRQSGCGRGNQRGGGGRGRSKG 120
           +E  N+  +   L+   + N+A   D+  N+ N  NN+   +   RG+   G   GR +G
Sbjct: 205 IEQLNSLTN---LTLNATANVAKKSDHRGNRFNSNNNWRGSNNNWRGSNFRGWRGGRGRG 264

Query: 121 RGYGSFNNGKPTCQF-NLHNSVQPSAFMA-------------NQNYNSYNLFVASSETVV 180
           R +      K TCQ   L N +    F               N    S+N F+AS  ++ 
Sbjct: 265 RSF------KTTCQVCGLDNHIAIDCFYRFDKTYSRSNHSANNDKQGSHNAFLASQNSIE 324

Query: 181 DPNWYADSGASNHVTVDYTNLTNPVDYEGKERVTVGDGNQLQITCVGSS---SLNAGRQS 240
           D +WY DSGASNHVT       N  ++ GK  + VG+G +L+I   GSS   SLN     
Sbjct: 325 DYDWYFDSGASNHVTHQTDKFQNLSEHHGKNSLIVGNGEKLEIVATGSSKLKSLNLHDIL 384

Query: 241 YGQANAERVLSEGLYRFDDGRTAAVEKS----KSVICSSGTLSRKNNVDLSVLTLSSSVN 300
           Y     + +LS      D+      +++    K  +     L       L  L+   S  
Sbjct: 385 YVPKITKNLLSVSKLAADNNILVEFDENCCFVKDKLTGKAILRGILKDGLYQLSEKDSSA 444

Query: 301 VVVSKVTWHRRVGHPSEKVFDLIVKQCNLPYRLNEKSTFCEAFQFGKAHALPFPHSVSRA 357
            V  K +WHR++GHP+ KV D+++K CN+    +++ +FCEA Q+GK H LPF  S S A
Sbjct: 445 YVSIKESWHRKLGHPNNKVLDIVLKSCNVKLSPSDQFSFCEACQYGKMHFLPFKTSFSHA 504

BLAST of Lag0005510 vs. ExPASy TrEMBL
Match: A0A6J1DCW4 (uncharacterized protein LOC111019598 OS=Momordica charantia OX=3673 GN=LOC111019598 PE=4 SV=1)

HSP 1 Score: 181.8 bits (460), Expect = 5.0e-42
Identity = 132/338 (39.05%), Postives = 181/338 (53.55%), Query Frame = 0

Query: 1   MKSHVDNLGQAGSPVSTRSLISQVLLGLDEEFNPVIAMIQGRGGITWSEMQAELLVYEKR 60
           MKSH DNL  AGS VS R L+SQVL GLDEE+NP++  +QG+  ++WSEM AELL YEKR
Sbjct: 175 MKSHADNLALAGSSVSVRDLVSQVLTGLDEEYNPIVVAVQGKVNLSWSEMHAELLTYEKR 234

Query: 61  LELQNTFKSSLML--SQGPSVN-------MANVKDNNNQKNRGNNYNRQSGCGRGNQRGG 120
           LE QN+ KS + +  +Q PSVN         N + NN   + G+N +R    G G QRG 
Sbjct: 235 LEYQNSLKSGIPINQTQTPSVNYVDGRSFQTNQRTNNGNNSHGSNTHR----GGGYQRGS 294

Query: 121 GGRGRSKGRGYGSFNNGKPTCQFNLHNSVQ-PSAFMANQNYNSYNLFVASSETVVDPNWY 180
            G+ R++GRG       +PT   N   S   P+ F A+    +    V + ETV+DP+WY
Sbjct: 295 FGQ-RNRGRG------PQPTQHKNFTPSNSGPNVFAAHHTSTT----VTTPETVIDPSWY 354

Query: 181 ADSGASNHVTVDYTNLTNPVDYEGKERVTVGDGNQLQITCVGSSSLNAGRQSY------- 240
           ADSGA++HVT +  N+   VDY G E V V +GN+L I+ +GS++++A   S        
Sbjct: 355 ADSGATSHVTANPNNVEQKVDYSGTENVIVANGNKLSISHIGSTNIHASGGSLKLKDVLR 414

Query: 241 ------------GQANAERVLSEGLYRFDDGR---------TAAVEKSKSVICSSGTLSR 300
                       G+   +  L + LYR D            TA +     V  S+ TLS 
Sbjct: 415 VPDIAKNLDKASGRTLLKGTLKDNLYRLDRSHRSPPATPTLTAPLFAHTVVSLSNNTLSS 474

BLAST of Lag0005510 vs. ExPASy TrEMBL
Match: A0A803PEH4 (Uncharacterized protein OS=Cannabis sativa OX=3483 PE=4 SV=1)

HSP 1 Score: 176.8 bits (447), Expect = 1.6e-40
Identity = 143/426 (33.57%), Postives = 202/426 (47.42%), Query Frame = 0

Query: 2   KSHVDNLGQAGSPVSTRSLISQVLLGLDEEFNPVIAMIQGRGGITWSEMQAELLVYEKRL 61
           K+  + L  AG P     L++ VL GLD E+  ++  I+ R   TW E+Q  LL ++ ++
Sbjct: 177 KNWSNMLALAGDPYPEAHLVANVLFGLDAEYLSIVVQIEARSNTTWQELQDLLLSFDSKI 236

Query: 62  E-LQN-TFKSSLMLSQGPSVNMANVKDNNNQKNRG---NNYNRQSGCGRGNQRGGGGRGR 121
           E LQN T  S+   S  P  NMA  K NNN + RG    N +  SG    N RG   R R
Sbjct: 237 ERLQNLTLNSNKATSSSPQANMA-AKTNNNGRGRGFQSQNASTNSGGLFSNSRGTSNRFR 296

Query: 122 SKGRGYGSFNNGKPTCQF-------------------------NLHNSVQPSAFMANQNY 181
            +GRG GS    +PTCQ                          N HN  +     A Q  
Sbjct: 297 GRGRGPGS--GSRPTCQVYGKYGHTAAVCYNRFDESYMGSDPNNPHNQNK-----AGQTN 356

Query: 182 NSYNLFVASSETVVDPNWYADSGASNHVTVDYTNLTNPVDYEGKERVTVGDGNQLQITCV 241
           N+++ FVA+ E +    W+ADSGASNH+T D  NLT   DY GKE V VG+G++L+IT +
Sbjct: 357 NNHSAFVATPEVLEFDAWFADSGASNHITSDPANLTQKQDYNGKESVVVGNGSKLRITHI 416

Query: 242 GSSSLNAGRQSY--------------------------------------------GQAN 301
           G+  LN    +Y                                             +  
Sbjct: 417 GNGKLNIESGNYLLLKDMLLVPKIAKNLVSVSKLATDNNVLIEFYSNFCLVKDKVTKKVL 476

Query: 302 AERVLSEGLYRFDDGRTAAVEK-SKSVICSSGTLSRKNNVDLSVLTLSSSVNVVVSKV-T 352
              VL + LY+ D   T +     +S   S+ T+S  +NV+      S + ++++S++  
Sbjct: 477 LHGVLKDELYQLDSPFTKSSHPYQQSNFLSAFTISVDSNVN-----QSQTDSLLISQMDV 536

BLAST of Lag0005510 vs. ExPASy TrEMBL
Match: A0A2K3LRE0 (Glutamate receptor OS=Trifolium pratense OX=57577 GN=L195_g037121 PE=4 SV=1)

HSP 1 Score: 176.0 bits (445), Expect = 2.8e-40
Identity = 127/378 (33.60%), Postives = 180/378 (47.62%), Query Frame = 0

Query: 1   MKSHVDNLGQAGSPVSTRSLISQVLLGLDEEFNPVIAMIQGRGGITWSEMQAELLVYEKR 60
           MK   D L  AGS +S   L+ Q L GLD ++NPV+  +  +  ++W E+QA+LL +E R
Sbjct: 146 MKGLADKLKLAGSAISNSDLVIQTLNGLDMDYNPVVVKLSDQIDLSWVELQAQLLAFESR 205

Query: 61  LELQNTFKSSLMLSQGPSVNMANVKDNNNQKNRGNNYNRQSGCGRGNQRGGGGRGRSKGR 120
           LE  N   +   L+   + N+A     N  K  GN +N      RGN RG   RG   GR
Sbjct: 206 LEQLNNINN---LNLNATANVA-----NKTKFSGNRFN-----SRGNWRGSNFRGMRGGR 265

Query: 121 GYGSFNNGKPTCQFNLHNSVQPSAF--------------MANQNYNSYNLFVASSETVVD 180
           G G F+N KP CQ    +    + F                N+   ++N FVAS     D
Sbjct: 266 GKGRFHNSKPVCQVCTGSHTAINCFYRFDKSYTGPNYSNSENEKQGTHNAFVASPYNGQD 325

Query: 181 PNWYADSGASNHVTVDYTNLTNPVDYEGKERVTVGDGNQLQITCVGSSSLNA---GRQSY 240
             WY DSGASNHVT       +  ++ GK  + VG+G +L+I   GSS +N        Y
Sbjct: 326 YEWYFDSGASNHVTHQADRFEDLTEHTGKNSLMVGNGEKLKIVASGSSKINTVNLHNVLY 385

Query: 241 GQANAERVLS--------EGLYRFDDGRTAAVEKSKSVICSSGTLSRKNNVDLSVLTLSS 300
                + +LS          +  FD       +K    +   G L +     LS  T ++
Sbjct: 386 VPQITKNLLSVSKLTSDNNAIVEFDHDYCYVKDKVTGKVLLKGRL-KDGLYQLSDATQTN 445

Query: 301 SVNVVVSKV--TWHRRVGHPSEKVFDLIVKQCNLPYRLNEKSTFCEAFQFGKAHALPFPH 352
             + V   V  +WHR++GHP+ K  D ++K CN+    +++  FCEA Q GK+H LPF  
Sbjct: 446 KDSCVYLSVKESWHRKLGHPNNKALDRVLKICNVKTSPSDQLEFCEACQLGKSHLLPFKS 505

BLAST of Lag0005510 vs. TAIR 10
Match: ATMG00300.1 (Gag-Pol-related retrotransposon family protein )

HSP 1 Score: 50.1 bits (118), Expect = 4.4e-06
Identity = 21/66 (31.82%), Postives = 33/66 (50.00%), Query Frame = 0

Query: 286 WHRRVGHPSEKVFDLIVKQCNLPYRLNEKSTFCEAFQFGKAHALPFPHSVSRASSVFELI 345
           WH R+ H S++  +L+VK+  L         FCE   +GK H + F        +  + +
Sbjct: 72  WHSRLAHMSQRGMELLVKKGFLDSSKVSSLKFCEDCIYGKTHRVNFSTGQHTTKNPLDYV 131

Query: 346 HADLWG 352
           H+DLWG
Sbjct: 132 HSDLWG 137