Lag0005331 (gene) Sponge gourd (AG‐4) v1

Overview


Name	Lag0005331
Type	gene
Organism	Luffa acutangula (Sponge gourd (AG‐4) v1)
Description	Small COPII coat GTPase SAR1
Location	chr6: 14450983 .. 14451814 (+)
RNA-Seq Expression	Lag0005331
Synteny	Lag0005331

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonCDSpolypeptide

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGTTGAAGGACGAGGTGAGTTTCATTTATTTTTCTTCACTCTGCAATTGAATTTTTGTCGTTGATTTTTTGATTTCTTATGGAAATTTTGAATTGTGTAGAGATTAGTGCAACACCACCCGACTCAACACCTGACCTCAGAGGAGTTAAGTATTGGGGAAATCAAATTCAAGGCTTTTGATCTTGGAGGCCACCGGATCGCTTGCAGAGTCTGGAAAGATTATTATGCCAAGGTGATCTTTAATTCATTTTTTGATTGCATTATGTCTGTGTAACCATGAATTGGCTTGATGATTTTCTTTGTCTTGAAATTGTCGATACTTTGATCTTTTCAATTCGATTTAGAAGCCTACATCCTGGTTTCTTTGGTTGGCCTCAACTTAATGACATTTTCCTCAACGACGGCTGTTATTATTGATCTTCCATTTTGAATTGATAACTGATTGGAACTATGATGTATAGTTTGTTTATTATTGATTTGATGGTTGTTGGAGTTTCCTTCTTGTCAAGCCCACCAAATGCCTTTGTTGTGCTTTGTTGTGCTTCATGGTAAGATTTAGACTTTGGTTTCAATCTTCTTCAGCGGGTAGTTGATTTTTCAAGTTGAAGCTTTTTGAATAACGAACCTCCTTTCATTTTCAGGGACTGGTAATTTCTGACCATATTTTTTAGTTATGGTAGTTTTGCATTGTATGTGCTGACAACGAAATTGCTTCTTATAATTGTGTAGAACTTCGTAAGCTTTGGCAAGCTTGCTCATGATCTTGTTATGCGCATCCAGAAAATTGATGGGGACAATTATCCTAAGGTTTGCCCAAACTATATTTTATAG

mRNA sequence

ATGTTGAAGGACGAGAGATTAGTGCAACACCACCCGACTCAACACCTGACCTCAGAGGAGTTAAGTATTGGGGAAATCAAATTCAAGGCTTTTGATCTTGGAGGCCACCGGATCGCTTGCAGAGTCTGGAAAGATTATTATGCCAAGAACTTCGTAAGCTTTGGCAAGCTTGCTCATGATCTTGTTATGCGCATCCAGAAAATTGATGGGGACAATTATCCTAAGGTTTGCCCAAACTATATTTTATAG

Coding sequence (CDS)

ATGTTGAAGGACGAGAGATTAGTGCAACACCACCCGACTCAACACCTGACCTCAGAGGAGTTAAGTATTGGGGAAATCAAATTCAAGGCTTTTGATCTTGGAGGCCACCGGATCGCTTGCAGAGTCTGGAAAGATTATTATGCCAAGAACTTCGTAAGCTTTGGCAAGCTTGCTCATGATCTTGTTATGCGCATCCAGAAAATTGATGGGGACAATTATCCTAAGGTTTGCCCAAACTATATTTTATAG

Protein sequence

MLKDERLVQHHPTQHLTSEELSIGEIKFKAFDLGGHRIACRVWKDYYAKNFVSFGKLAHDLVMRIQKIDGDNYPKVCPNYIL

Homology

[Close]

BLAST of Lag0005331 vs. NCBI nr
Match: KAF2590872.1 (hypothetical protein F2Q70_00039840 [Brassica cretica])

HSP 1 Score: 100.5 bits (249), Expect = 7.0e-18
Identity = 46/65 (70.77%), Postives = 52/65 (80.00%), Query Frame = 0


Query: 1   MLKDERLVQHHPTQHLTSEELSIGEIKFKAFDLGGHRIACRVWKDYYAKNFVSFGKLAHD 60
           MLKDERLVQH PTQH TSEELSIG+IKFKAFDLGGH+IA RVWKDYYAK F+      H 
Sbjct: 39  MLKDERLVQHQPTQHPTSEELSIGKIKFKAFDLGGHQIARRVWKDYYAKVFIHLSVFCHH 98

Query: 61  LVMRI 66
            ++ +
Sbjct: 99  NIILV 103

[Close]

BLAST of Lag0005331 vs. NCBI nr
Match: XP_020208105.1 (GTP-binding protein SAR1B [Cajanus cajan])

HSP 1 Score: 98.6 bits (244), Expect = 2.7e-17
Identity = 45/55 (81.82%), Postives = 49/55 (89.09%), Query Frame = 0


Query: 1  MLKDERLVQHHPTQHLTSEELSIGEIKFKAFDLGGHRIACRVWKDYYAKNFVSFG 56
          MLKDERLVQH PTQH TSEELSIG+IKFKAFDLGGH++A RVWKDYYAK F  +G
Sbjct: 39 MLKDERLVQHQPTQHPTSEELSIGKIKFKAFDLGGHQVARRVWKDYYAKGFSMWG 93

[Close]

BLAST of Lag0005331 vs. NCBI nr
Match: EFH70897.1 (secretion-associated ras [Arabidopsis lyrata subsp. lyrata])

HSP 1 Score: 98.2 bits (243), Expect = 3.5e-17
Identity = 47/61 (77.05%), Postives = 50/61 (81.97%), Query Frame = 0


Query: 1  MLKDERLVQHHPTQHLTSEELSIGEIKFKAFDLGGHRIACRVWKDYYAKNFVSFGKLAHD 60
          MLKDERLVQH PTQH TSEELSIG+IKFKAFDLGGH+IA RVWKDYYAK  + F  L   
Sbjct: 39 MLKDERLVQHQPTQHPTSEELSIGKIKFKAFDLGGHQIARRVWKDYYAKRNMVFSLLIES 98

Query: 61 L 62
          L
Sbjct: 99 L 99

[Close]

BLAST of Lag0005331 vs. NCBI nr
Match: CBI22905.3 (unnamed protein product, partial [Vitis vinifera])

HSP 1 Score: 98.2 bits (243), Expect = 3.5e-17
Identity = 46/56 (82.14%), Postives = 49/56 (87.50%), Query Frame = 0


Query: 1  MLKDERLVQHHPTQHLTSEELSIGEIKFKAFDLGGHRIACRVWKDYYAKNFVSFGK 57
          MLKDERLVQH PTQH TSEELSIG+IKFKAFDLGGH+IA RVWKDYYAK +   GK
Sbjct: 39 MLKDERLVQHQPTQHPTSEELSIGKIKFKAFDLGGHQIARRVWKDYYAKRYGEDGK 94

[Close]

BLAST of Lag0005331 vs. NCBI nr
Match: MBA0679344.1 (hypothetical protein [Gossypium aridum])

HSP 1 Score: 97.8 bits (242), Expect = 4.5e-17
Identity = 45/54 (83.33%), Postives = 49/54 (90.74%), Query Frame = 0


Query: 1  MLKDERLVQHHPTQHLTSEELSIGEIKFKAFDLGGHRIACRVWKDYYAKNFVSF 55
          MLKDERLVQH PTQ+ TSEELSIG+IKFKAFDLGGH+IA RVWKDYYAK F+ F
Sbjct: 39 MLKDERLVQHQPTQYPTSEELSIGKIKFKAFDLGGHQIARRVWKDYYAKTFIVF 92

[Close]

BLAST of Lag0005331 vs. ExPASy Swiss-Prot
Match: O04834 (GTP-binding protein SAR1A OS=Arabidopsis thaliana OX=3702 GN=SAR1A PE=2 SV=1)

HSP 1 Score: 94.7 bits (234), Expect = 5.0e-19
Identity = 44/49 (89.80%), Postives = 46/49 (93.88%), Query Frame = 0


Query: 1  MLKDERLVQHHPTQHLTSEELSIGEIKFKAFDLGGHRIACRVWKDYYAK 50
          MLKDERLVQH PTQH TSEELSIG+IKFKAFDLGGH+IA RVWKDYYAK
Sbjct: 39 MLKDERLVQHQPTQHPTSEELSIGKIKFKAFDLGGHQIARRVWKDYYAK 87

[Close]

BLAST of Lag0005331 vs. ExPASy Swiss-Prot
Match: O04266 (GTP-binding protein SAR1A OS=Brassica campestris OX=3711 GN=SAR1A PE=2 SV=1)

HSP 1 Score: 94.7 bits (234), Expect = 5.0e-19
Identity = 44/49 (89.80%), Postives = 46/49 (93.88%), Query Frame = 0


Query: 1  MLKDERLVQHHPTQHLTSEELSIGEIKFKAFDLGGHRIACRVWKDYYAK 50
          MLKDERLVQH PTQH TSEELSIG+IKFKAFDLGGH+IA RVWKDYYAK
Sbjct: 39 MLKDERLVQHQPTQHPTSEELSIGKIKFKAFDLGGHQIARRVWKDYYAK 87

[Close]

BLAST of Lag0005331 vs. ExPASy Swiss-Prot
Match: Q01474 (GTP-binding protein SAR1B OS=Arabidopsis thaliana OX=3702 GN=SAR1B PE=1 SV=1)

HSP 1 Score: 94.7 bits (234), Expect = 5.0e-19
Identity = 44/49 (89.80%), Postives = 46/49 (93.88%), Query Frame = 0


Query: 1  MLKDERLVQHHPTQHLTSEELSIGEIKFKAFDLGGHRIACRVWKDYYAK 50
          MLKDERLVQH PTQH TSEELSIG+IKFKAFDLGGH+IA RVWKDYYAK
Sbjct: 39 MLKDERLVQHQPTQHPTSEELSIGKIKFKAFDLGGHQIARRVWKDYYAK 87

[Close]

BLAST of Lag0005331 vs. ExPASy Swiss-Prot
Match: O04267 (GTP-binding protein SAR1B OS=Brassica campestris OX=3711 GN=SAR1B PE=2 SV=1)

HSP 1 Score: 94.7 bits (234), Expect = 5.0e-19
Identity = 44/49 (89.80%), Postives = 46/49 (93.88%), Query Frame = 0


Query: 1  MLKDERLVQHHPTQHLTSEELSIGEIKFKAFDLGGHRIACRVWKDYYAK 50
          MLKDERLVQH PTQH TSEELSIG+IKFKAFDLGGH+IA RVWKDYYAK
Sbjct: 39 MLKDERLVQHQPTQHPTSEELSIGKIKFKAFDLGGHQIARRVWKDYYAK 87

[Close]

BLAST of Lag0005331 vs. ExPASy Swiss-Prot
Match: P52885 (GTP-binding protein SAR1 OS=Nicotiana tabacum OX=4097 GN=SAR1 PE=2 SV=1)

HSP 1 Score: 92.4 bits (228), Expect = 2.5e-18
Identity = 43/49 (87.76%), Postives = 46/49 (93.88%), Query Frame = 0


Query: 1  MLKDERLVQHHPTQHLTSEELSIGEIKFKAFDLGGHRIACRVWKDYYAK 50
          MLKDERLVQH PTQ+ TSEELSIG+IKFKAFDLGGH+IA RVWKDYYAK
Sbjct: 39 MLKDERLVQHQPTQYPTSEELSIGKIKFKAFDLGGHQIARRVWKDYYAK 87

The following BLAST results are available for this feature:

BLAST of Lag0005331 vs. NCBI nr


Match Name	E-value	Identity	Description
KAF2590872.1	7.0e-18	70.77	hypothetical protein F2Q70_00039840 [Brassica cretica]	[more]
XP_020208105.1	2.7e-17	81.82	GTP-binding protein SAR1B [Cajanus cajan]	[more]
EFH70897.1	3.5e-17	77.05	secretion-associated ras [Arabidopsis lyrata subsp. lyrata]	[more]
CBI22905.3	3.5e-17	82.14	unnamed protein product, partial [Vitis vinifera]	[more]
MBA0679344.1	4.5e-17	83.33	hypothetical protein [Gossypium aridum]	[more]

BLAST of Lag0005331 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
O04834	5.0e-19	89.80	GTP-binding protein SAR1A OS=Arabidopsis thaliana OX=3702 GN=SAR1A PE=2 SV=1	[more]
O04266	5.0e-19	89.80	GTP-binding protein SAR1A OS=Brassica campestris OX=3711 GN=SAR1A PE=2 SV=1	[more]
Q01474	5.0e-19	89.80	GTP-binding protein SAR1B OS=Arabidopsis thaliana OX=3702 GN=SAR1B PE=1 SV=1	[more]
O04267	5.0e-19	89.80	GTP-binding protein SAR1B OS=Brassica campestris OX=3711 GN=SAR1B PE=2 SV=1	[more]
P52885	2.5e-18	87.76	GTP-binding protein SAR1 OS=Nicotiana tabacum OX=4097 GN=SAR1 PE=2 SV=1	[more]

InterPro

Analysis Name: InterPro Annotations of Sponge gourd (AG-4) v1
Date Performed: 2022-08-01


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR006689	Small GTPase superfamily, ARF/SAR type	PRINTS	PR00328	SAR1GTPBP	coord: 12..36 score: 81.88 coord: 39..64 score: 35.97
IPR006689	Small GTPase superfamily, ARF/SAR type	PFAM	PF00025	Arf	coord: 1..49 e-value: 3.9E-13 score: 49.2
IPR027417	P-loop containing nucleoside triphosphate hydrolase	GENE3D	3.40.50.300		coord: 1..68 e-value: 2.8E-11 score: 45.3
IPR027417	P-loop containing nucleoside triphosphate hydrolase	SUPERFAMILY	52540	P-loop containing nucleoside triphosphate hydrolases	coord: 3..48
None	No IPR available	PANTHER	PTHR45684:SF29	GTP-BINDING PROTEIN SAR1-LIKE	coord: 1..55
IPR006687	Small GTPase superfamily, SAR1-type	PANTHER	PTHR45684	RE74312P	coord: 1..55

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
Lag0005331.1	Lag0005331.1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0006886	intracellular protein transport
biological_process	GO:0016192	vesicle-mediated transport
cellular_component	GO:0005783	endoplasmic reticulum
cellular_component	GO:0005794	Golgi apparatus
cellular_component	GO:0016021	integral component of membrane
molecular_function	GO:0003924	GTPase activity
molecular_function	GO:0005525	GTP binding