Lag0005327 (gene) Sponge gourd (AG‐4) v1

Overview
NameLag0005327
Typegene
OrganismLuffa acutangula (Sponge gourd (AG‐4) v1)
Descriptionpolygalacturonase 1 beta-like protein 3
Locationchr6: 14356943 .. 14358893 (+)
RNA-Seq ExpressionLag0005327
SyntenyLag0005327
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonCDSpolypeptide
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGCGCAGATTACCCGCGCCGCTTGTGGTTCCCCTCTCTCTCTTTATCTTCTTGCTTTCGGCCCTCAATGTAAGTCAATCTCCATCTCCATTTCACACTTTCTTTCTTTTCCTCTTGTCAATTCTGAATTGTAAATTGTAAATTGCAGGTTTCGGCGGCCGGCGACGAGGATAATCCCTTTACTCCCAAAGCTTTTCTCAATCGTTATTGGAACAAGGAGGTCCGGAATGATTTGCCAAAACCCAGTTTCCTCTTCTCCAAGGTCTCTCCCTTGACGGCAGTGCAGTCGGCCACCTTCGCCAAGCTCGCAGCCGAAAACGCACTCTCCTCCCGCCTCCCCGAGTTCTGCTCCGCCGCTAATTTGCTCTGCTTCCCCGATTTGTCCCCCTCTTTGGCCAAGCACGACAGGGACTCCAACTTTGCCGTCTATTCCAATCGGAACTTCACCGCTTACGGCACCCAGAGATCTGGTGGAATCGACTCCTTCAAGAACTATTCCAATGACGACAATGTCATCGTGGATTCCTTCCGCCGCTACAGCCACGAGGCATTGGGCCACAAGGACCAATTTACGACCTATGCCACCGGCACCAACGTCCCCGACCAAAGTTTCAACACTTATGGTTCCAAAGCCGTCGGAGGCGACGGCAAGTTCACGACCTACGAGGAATCCGTCAATGTCCCCAATCTCCGATTTACCTCCTACTCCTCCGACGCCACGGGAAGATCTCACACATTCAAGAGCTACAGTGAGAACGCAAACGCCGGAGATCAGTCGTTTGCCAACTACGGAAAACGTGGTAATGGGGGTCCCAACGAGTTCGCTGGTTACGGGACCAACGCAAACGTAGTAGGCTCGGGGTTTTCGAGCTACGGGTCTGAGGGAAATGGGGCCAACGACACATTCACCAATTACGGGAACGAGCAGAACAACCCACAGAATAACTTCAGGAACTATGGCGAGGGTGGGAATGGAGCGATTGAAAGCTTCTCCAGTTACAGGGACCAGGCGAATGTGGGGGATGATTCATTCCAATCCTACTCCAAGAACTCGAACTCAGCGAAGGTCAATTTCGCAAATTACGGGAAGTCGTTCAACGAGGGCACGGACAAATTCAGTGGGTATGGTCAGAATGCGAATGGGCAAAGCGTGGGATTCAAGATCTATGGGGTGAATACGACGTTCAAAGATTACGCGAAACAGGGGATTAGTTTTGCGAAATACAGCAACGTGAGCAGTGGGGGTGGGAAAATGTCGGTGAGCGGGAGTTTGGCGAAGAGGTGGGTAGAGCCCGGGAAGTTCTTCAGGGAGTCGATGCTGAAGAAGGGGACGGTGATGGCGATGCCGGACATCAGAGATAAAATGCCGAAGAGGTCGTTCTTGCCTCGATCTATAGTGTCGAAGTTGCCGTTTTCGAGTTCGAAAGTGGGAGTGATGAAGGAGATATTCCAGGCGGGGGAGAACTCGTCGATGGAGACGATGATGACGGAGACGCTGAGGGAGTGCGAGAGGGCTCCGAGCGCGGGTGAGACGAAGCGGTGCGTGGGGTCGGTGGAAGACATGATAGACTTCGCGACGTCGGTGCTGGGGCGGAACGTGGTGGTCCGGACGACGGAGAACACGAAGGGTTCGAAGGGGAAAGTAATGATTGGGGCGGTGAAGGGGATAAACGGAGGGAAGGTGACGCAGTCGGTGTCGTGCCACCAGAGCCTGTTCCCTTACCTACTCTACTACTGCCACTCGGTGCCCAAGGTTCGGGTTTACGAAGCGGATTTGCTGGATCCGAGCTCTAAGGCCACCATCAACCACGGAGTTGCGATCTGTCACTTGGACACCTCGGCCTGGAGTCCCGCTCACGGGGCCTTCATAGCTCTCGGCTCCGCTCCCGGTCGGGTCGAGGTGTGCCACTGGATCTTCGAGAACGACATGACCTGGGCCGTCGCCGACTGA

mRNA sequence

ATGCGCAGATTACCCGCGCCGCTTGTGGTTCCCCTCTCTCTCTTTATCTTCTTGCTTTCGGCCCTCAATGTTTCGGCGGCCGGCGACGAGGATAATCCCTTTACTCCCAAAGCTTTTCTCAATCGTTATTGGAACAAGGAGGTCCGGAATGATTTGCCAAAACCCAGTTTCCTCTTCTCCAAGGTCTCTCCCTTGACGGCAGTGCAGTCGGCCACCTTCGCCAAGCTCGCAGCCGAAAACGCACTCTCCTCCCGCCTCCCCGAGTTCTGCTCCGCCGCTAATTTGCTCTGCTTCCCCGATTTGTCCCCCTCTTTGGCCAAGCACGACAGGGACTCCAACTTTGCCGTCTATTCCAATCGGAACTTCACCGCTTACGGCACCCAGAGATCTGGTGGAATCGACTCCTTCAAGAACTATTCCAATGACGACAATGTCATCGTGGATTCCTTCCGCCGCTACAGCCACGAGGCATTGGGCCACAAGGACCAATTTACGACCTATGCCACCGGCACCAACGTCCCCGACCAAAGTTTCAACACTTATGGTTCCAAAGCCGTCGGAGGCGACGGCAAGTTCACGACCTACGAGGAATCCGTCAATGTCCCCAATCTCCGATTTACCTCCTACTCCTCCGACGCCACGGGAAGATCTCACACATTCAAGAGCTACAGTGAGAACGCAAACGCCGGAGATCAGTCGTTTGCCAACTACGGAAAACGTGGTAATGGGGGTCCCAACGAGTTCGCTGGTTACGGGACCAACGCAAACGTAGTAGGCTCGGGGTTTTCGAGCTACGGGTCTGAGGGAAATGGGGCCAACGACACATTCACCAATTACGGGAACGAGCAGAACAACCCACAGAATAACTTCAGGAACTATGGCGAGGGTGGGAATGGAGCGATTGAAAGCTTCTCCAGTTACAGGGACCAGGCGAATGTGGGGGATGATTCATTCCAATCCTACTCCAAGAACTCGAACTCAGCGAAGGTCAATTTCGCAAATTACGGGAAGTCGTTCAACGAGGGCACGGACAAATTCAGTGGGTATGGTCAGAATGCGAATGGGCAAAGCGTGGGATTCAAGATCTATGGGGTGAATACGACGTTCAAAGATTACGCGAAACAGGGGATTAGTTTTGCGAAATACAGCAACGTGAGCAGTGGGGGTGGGAAAATGTCGGTGAGCGGGAGTTTGGCGAAGAGGTGGGTAGAGCCCGGGAAGTTCTTCAGGGAGTCGATGCTGAAGAAGGGGACGGTGATGGCGATGCCGGACATCAGAGATAAAATGCCGAAGAGGTCGTTCTTGCCTCGATCTATAGTGTCGAAGTTGCCGTTTTCGAGTTCGAAAGTGGGAGTGATGAAGGAGATATTCCAGGCGGGGGAGAACTCGTCGATGGAGACGATGATGACGGAGACGCTGAGGGAGTGCGAGAGGGCTCCGAGCGCGGGTGAGACGAAGCGGTGCGTGGGGTCGGTGGAAGACATGATAGACTTCGCGACGTCGGTGCTGGGGCGGAACGTGGTGGTCCGGACGACGGAGAACACGAAGGGTTCGAAGGGGAAAGTAATGATTGGGGCGGTGAAGGGGATAAACGGAGGGAAGGTGACGCAGTCGGTGTCGTGCCACCAGAGCCTGTTCCCTTACCTACTCTACTACTGCCACTCGGTGCCCAAGGTTCGGGTTTACGAAGCGGATTTGCTGGATCCGAGCTCTAAGGCCACCATCAACCACGGAGTTGCGATCTGTCACTTGGACACCTCGGCCTGGAGTCCCGCTCACGGGGCCTTCATAGCTCTCGGCTCCGCTCCCGGTCGGGTCGAGGTGTGCCACTGGATCTTCGAGAACGACATGACCTGGGCCGTCGCCGACTGA

Coding sequence (CDS)

ATGCGCAGATTACCCGCGCCGCTTGTGGTTCCCCTCTCTCTCTTTATCTTCTTGCTTTCGGCCCTCAATGTTTCGGCGGCCGGCGACGAGGATAATCCCTTTACTCCCAAAGCTTTTCTCAATCGTTATTGGAACAAGGAGGTCCGGAATGATTTGCCAAAACCCAGTTTCCTCTTCTCCAAGGTCTCTCCCTTGACGGCAGTGCAGTCGGCCACCTTCGCCAAGCTCGCAGCCGAAAACGCACTCTCCTCCCGCCTCCCCGAGTTCTGCTCCGCCGCTAATTTGCTCTGCTTCCCCGATTTGTCCCCCTCTTTGGCCAAGCACGACAGGGACTCCAACTTTGCCGTCTATTCCAATCGGAACTTCACCGCTTACGGCACCCAGAGATCTGGTGGAATCGACTCCTTCAAGAACTATTCCAATGACGACAATGTCATCGTGGATTCCTTCCGCCGCTACAGCCACGAGGCATTGGGCCACAAGGACCAATTTACGACCTATGCCACCGGCACCAACGTCCCCGACCAAAGTTTCAACACTTATGGTTCCAAAGCCGTCGGAGGCGACGGCAAGTTCACGACCTACGAGGAATCCGTCAATGTCCCCAATCTCCGATTTACCTCCTACTCCTCCGACGCCACGGGAAGATCTCACACATTCAAGAGCTACAGTGAGAACGCAAACGCCGGAGATCAGTCGTTTGCCAACTACGGAAAACGTGGTAATGGGGGTCCCAACGAGTTCGCTGGTTACGGGACCAACGCAAACGTAGTAGGCTCGGGGTTTTCGAGCTACGGGTCTGAGGGAAATGGGGCCAACGACACATTCACCAATTACGGGAACGAGCAGAACAACCCACAGAATAACTTCAGGAACTATGGCGAGGGTGGGAATGGAGCGATTGAAAGCTTCTCCAGTTACAGGGACCAGGCGAATGTGGGGGATGATTCATTCCAATCCTACTCCAAGAACTCGAACTCAGCGAAGGTCAATTTCGCAAATTACGGGAAGTCGTTCAACGAGGGCACGGACAAATTCAGTGGGTATGGTCAGAATGCGAATGGGCAAAGCGTGGGATTCAAGATCTATGGGGTGAATACGACGTTCAAAGATTACGCGAAACAGGGGATTAGTTTTGCGAAATACAGCAACGTGAGCAGTGGGGGTGGGAAAATGTCGGTGAGCGGGAGTTTGGCGAAGAGGTGGGTAGAGCCCGGGAAGTTCTTCAGGGAGTCGATGCTGAAGAAGGGGACGGTGATGGCGATGCCGGACATCAGAGATAAAATGCCGAAGAGGTCGTTCTTGCCTCGATCTATAGTGTCGAAGTTGCCGTTTTCGAGTTCGAAAGTGGGAGTGATGAAGGAGATATTCCAGGCGGGGGAGAACTCGTCGATGGAGACGATGATGACGGAGACGCTGAGGGAGTGCGAGAGGGCTCCGAGCGCGGGTGAGACGAAGCGGTGCGTGGGGTCGGTGGAAGACATGATAGACTTCGCGACGTCGGTGCTGGGGCGGAACGTGGTGGTCCGGACGACGGAGAACACGAAGGGTTCGAAGGGGAAAGTAATGATTGGGGCGGTGAAGGGGATAAACGGAGGGAAGGTGACGCAGTCGGTGTCGTGCCACCAGAGCCTGTTCCCTTACCTACTCTACTACTGCCACTCGGTGCCCAAGGTTCGGGTTTACGAAGCGGATTTGCTGGATCCGAGCTCTAAGGCCACCATCAACCACGGAGTTGCGATCTGTCACTTGGACACCTCGGCCTGGAGTCCCGCTCACGGGGCCTTCATAGCTCTCGGCTCCGCTCCCGGTCGGGTCGAGGTGTGCCACTGGATCTTCGAGAACGACATGACCTGGGCCGTCGCCGACTGA

Protein sequence

MRRLPAPLVVPLSLFIFLLSALNVSAAGDEDNPFTPKAFLNRYWNKEVRNDLPKPSFLFSKVSPLTAVQSATFAKLAAENALSSRLPEFCSAANLLCFPDLSPSLAKHDRDSNFAVYSNRNFTAYGTQRSGGIDSFKNYSNDDNVIVDSFRRYSHEALGHKDQFTTYATGTNVPDQSFNTYGSKAVGGDGKFTTYEESVNVPNLRFTSYSSDATGRSHTFKSYSENANAGDQSFANYGKRGNGGPNEFAGYGTNANVVGSGFSSYGSEGNGANDTFTNYGNEQNNPQNNFRNYGEGGNGAIESFSSYRDQANVGDDSFQSYSKNSNSAKVNFANYGKSFNEGTDKFSGYGQNANGQSVGFKIYGVNTTFKDYAKQGISFAKYSNVSSGGGKMSVSGSLAKRWVEPGKFFRESMLKKGTVMAMPDIRDKMPKRSFLPRSIVSKLPFSSSKVGVMKEIFQAGENSSMETMMTETLRECERAPSAGETKRCVGSVEDMIDFATSVLGRNVVVRTTENTKGSKGKVMIGAVKGINGGKVTQSVSCHQSLFPYLLYYCHSVPKVRVYEADLLDPSSKATINHGVAICHLDTSAWSPAHGAFIALGSAPGRVEVCHWIFENDMTWAVAD
Homology
BLAST of Lag0005327 vs. NCBI nr
Match: XP_038884819.1 (polygalacturonase 1 beta-like protein 3 [Benincasa hispida])

HSP 1 Score: 1149.4 bits (2972), Expect = 0.0e+00
Identity = 575/623 (92.30%), Postives = 598/623 (95.99%), Query Frame = 0

Query: 1   MRRLPAPLVVPLSLFIFLLSALNVSAAGDEDNPFTPKAFLNRYWNKEVRNDLPKPSFLFS 60
           M RLP PLVVPL LFIF LSALNV AAGDEDNPF+PKAFLNRYWNKEVRND PKPSFLFS
Sbjct: 1   MHRLPTPLVVPLYLFIFSLSALNVPAAGDEDNPFSPKAFLNRYWNKEVRNDFPKPSFLFS 60

Query: 61  KVSPLTAVQSATFAKLAAENALSSRLPEFCSAANLLCFPDLSPSLAKHDRDSNFAVYSNR 120
           K SPLTAVQSA FAKLAA+NALSSRLP+FCSAANLLCFPDLSPSLAKHDRDSNFAVYSN+
Sbjct: 61  KASPLTAVQSAAFAKLAAQNALSSRLPDFCSAANLLCFPDLSPSLAKHDRDSNFAVYSNK 120

Query: 121 NFTAYGTQRSGGIDSFKNYSNDDNVIVDSFRRYSHEALGHKDQFTTYATGTNVPDQSFNT 180
           NFT YGTQRSGGIDSFKNYSN +NVIVDSFRRYSHEALGH DQF+TYAT TNVPDQSFNT
Sbjct: 121 NFTTYGTQRSGGIDSFKNYSNGENVIVDSFRRYSHEALGHTDQFSTYATDTNVPDQSFNT 180

Query: 181 YGSKAVGGDGKFTTYEESVNVPNLRFTSYSSDATGRSHTFKSYSENANAGDQSFANYGKR 240
           Y SKA+GGDGKFTTYE+SVNVPNLRFT+YSSDATGRS +FKSYSENANAGDQSFANYGKR
Sbjct: 181 YASKAIGGDGKFTTYEQSVNVPNLRFTTYSSDATGRSQSFKSYSENANAGDQSFANYGKR 240

Query: 241 GNGGPNEFAGYGTNANVVGSGFSSYGSEGNGANDTFTNYGNEQNNPQNNFRNYGEGGNGA 300
           GNGGPNEF GYGTNANVVGSGFSSYG++GNGANDTFTNYGN QNNPQNNFRNYGEGGNGA
Sbjct: 241 GNGGPNEFTGYGTNANVVGSGFSSYGTDGNGANDTFTNYGNNQNNPQNNFRNYGEGGNGA 300

Query: 301 IESFSSYRDQANVGDDSFQSYSKNSNSAKVNFANYGKSFNEGTDKFSGYGQNANGQSVGF 360
           IESFSSYRDQANVGDDSFQSYSKNSNSAKV FANYGKSFNEGTDKFSGYGQN NGQSVGF
Sbjct: 301 IESFSSYRDQANVGDDSFQSYSKNSNSAKVKFANYGKSFNEGTDKFSGYGQNGNGQSVGF 360

Query: 361 KIYGVNTTFKDYAKQGISFAKYSNVSSGGGKMSVSGSLAKRWVEPGKFFRESMLKKGTVM 420
           K+YGVNTTFKDYAKQGISFAKYSNVSSGG K SV GSLAKRWVEPGKFFRESML+KGTVM
Sbjct: 361 KMYGVNTTFKDYAKQGISFAKYSNVSSGGAKTSVRGSLAKRWVEPGKFFRESMLRKGTVM 420

Query: 421 AMPDIRDKMPKRSFLPRSIVSKLPFSSSKVGVMKEIFQAGENSSMETMMTETLRECERAP 480
           AMPDIRDKMPKRSFLPRSI+SKLPFSSSK+GVMK+IF AG+NSSMETMMTET+RECERAP
Sbjct: 421 AMPDIRDKMPKRSFLPRSILSKLPFSSSKLGVMKQIFHAGDNSSMETMMTETVRECERAP 480

Query: 481 SAGETKRCVGSVEDMIDFATSVLGRNVVVRTTENTKGSKGKVMIGAVKGINGGKVTQSVS 540
           SAGETKRCVGSVEDMIDFATSVLGRNVVVRTTENTKGSKG +MIGAVKGINGGKVT+SVS
Sbjct: 481 SAGETKRCVGSVEDMIDFATSVLGRNVVVRTTENTKGSKGNIMIGAVKGINGGKVTESVS 540

Query: 541 CHQSLFPYLLYYCHSVPKVRVYEADLLDPSSKATINHGVAICHLDTSAWSPAHGAFIALG 600
           CHQSLFPYLLYYCHSVPKVRVYEADLLDP+SKATINHGVAICHLDTSAWSPAHGAFIAL 
Sbjct: 541 CHQSLFPYLLYYCHSVPKVRVYEADLLDPTSKATINHGVAICHLDTSAWSPAHGAFIALS 600

Query: 601 SAPGRVEVCHWIFENDMTWAVAD 624
           SAPG+VEVCHWIFENDMTW VAD
Sbjct: 601 SAPGQVEVCHWIFENDMTWTVAD 623

BLAST of Lag0005327 vs. NCBI nr
Match: XP_008444317.1 (PREDICTED: polygalacturonase 1 beta-like protein 3 [Cucumis melo] >KAA0061089.1 polygalacturonase 1 beta-like protein 3 [Cucumis melo var. makuwa])

HSP 1 Score: 1132.5 bits (2928), Expect = 0.0e+00
Identity = 565/623 (90.69%), Postives = 593/623 (95.18%), Query Frame = 0

Query: 1   MRRLPAPLVVPLSLFIFLLSALNVSAAGDEDNPFTPKAFLNRYWNKEVRNDLPKPSFLFS 60
           M RLP   VVPL LFIF LSALNV A GDEDNPFTPKAFLNRYWNKEVRND+PKPSFLFS
Sbjct: 1   MHRLPTLHVVPLYLFIFSLSALNVPAVGDEDNPFTPKAFLNRYWNKEVRNDIPKPSFLFS 60

Query: 61  KVSPLTAVQSATFAKLAAENALSSRLPEFCSAANLLCFPDLSPSLAKHDRDSNFAVYSNR 120
           K SPLTAVQSA FAKLAAENALSSRLP+FCSAANLLCFPDLSPSLAKHDRDSNFAVYSN+
Sbjct: 61  KASPLTAVQSAAFAKLAAENALSSRLPDFCSAANLLCFPDLSPSLAKHDRDSNFAVYSNK 120

Query: 121 NFTAYGTQRSGGIDSFKNYSNDDNVIVDSFRRYSHEALGHKDQFTTYATGTNVPDQSFNT 180
           NFT YGTQRSGGIDSFKNYSN DNV+VDSFRRYSHEALGH D+F+TY T TNVPDQSFNT
Sbjct: 121 NFTTYGTQRSGGIDSFKNYSNGDNVVVDSFRRYSHEALGHTDRFSTYGTETNVPDQSFNT 180

Query: 181 YGSKAVGGDGKFTTYEESVNVPNLRFTSYSSDATGRSHTFKSYSENANAGDQSFANYGKR 240
           Y SKA+GGDGKFTTYE+SVNVPNLRFT+YSSDATGRS  FKSYSENANAGDQSF NYGKR
Sbjct: 181 YASKAIGGDGKFTTYEQSVNVPNLRFTTYSSDATGRSQAFKSYSENANAGDQSFVNYGKR 240

Query: 241 GNGGPNEFAGYGTNANVVGSGFSSYGSEGNGANDTFTNYGNEQNNPQNNFRNYGEGGNGA 300
           GNGGPNEF GYGT+ANVVGS FSSYG++GNGANDTFTNYGN+QNNPQNNFR+YGEGGNGA
Sbjct: 241 GNGGPNEFTGYGTSANVVGSRFSSYGTDGNGANDTFTNYGNDQNNPQNNFRSYGEGGNGA 300

Query: 301 IESFSSYRDQANVGDDSFQSYSKNSNSAKVNFANYGKSFNEGTDKFSGYGQNANGQSVGF 360
           IESFSSYRDQANVGDDSFQSYSKNSNSAKVNFA+YGKSFNEGTDKFSGYGQN NGQSVGF
Sbjct: 301 IESFSSYRDQANVGDDSFQSYSKNSNSAKVNFASYGKSFNEGTDKFSGYGQNGNGQSVGF 360

Query: 361 KIYGVNTTFKDYAKQGISFAKYSNVSSGGGKMSVSGSLAKRWVEPGKFFRESMLKKGTVM 420
           K+YGVNTTFKDYAKQGI+FAKYSNVSSGG K SVSGSLAKRWVEPGKFFRESML+KG+VM
Sbjct: 361 KVYGVNTTFKDYAKQGINFAKYSNVSSGGAKTSVSGSLAKRWVEPGKFFRESMLRKGSVM 420

Query: 421 AMPDIRDKMPKRSFLPRSIVSKLPFSSSKVGVMKEIFQAGENSSMETMMTETLRECERAP 480
           AMPDIRDKMPKRSFLPRSI+SKLPFSSSK+GVMK+IF AG+NSSMETMMTET+RECER P
Sbjct: 421 AMPDIRDKMPKRSFLPRSILSKLPFSSSKLGVMKQIFHAGDNSSMETMMTETVRECERVP 480

Query: 481 SAGETKRCVGSVEDMIDFATSVLGRNVVVRTTENTKGSKGKVMIGAVKGINGGKVTQSVS 540
           SAGETKRCVGSVEDMIDFATSVLGRNVVVRTTENTKGSKG +MIG VKG+NGGKVT+SVS
Sbjct: 481 SAGETKRCVGSVEDMIDFATSVLGRNVVVRTTENTKGSKGNIMIGKVKGMNGGKVTESVS 540

Query: 541 CHQSLFPYLLYYCHSVPKVRVYEADLLDPSSKATINHGVAICHLDTSAWSPAHGAFIALG 600
           CHQSLFPYLLYYCHSVPKVRVYE DLLDP+SKATINHGVAICHLDTSAWSPAHGAFIALG
Sbjct: 541 CHQSLFPYLLYYCHSVPKVRVYEVDLLDPASKATINHGVAICHLDTSAWSPAHGAFIALG 600

Query: 601 SAPGRVEVCHWIFENDMTWAVAD 624
           SAPGRVEVCHWIFENDMTW VAD
Sbjct: 601 SAPGRVEVCHWIFENDMTWTVAD 623

BLAST of Lag0005327 vs. NCBI nr
Match: XP_023546090.1 (polygalacturonase 1 beta-like protein 3 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 1131.7 bits (2926), Expect = 0.0e+00
Identity = 569/623 (91.33%), Postives = 593/623 (95.18%), Query Frame = 0

Query: 1   MRRLPAPLVVPLSLFIFLLSALNVSAAGDEDNPFTPKAFLNRYWNKEVRNDLPKPSFLFS 60
           M  LPAPL+V L LFIFLLS LNV AA DEDNPFTPKAFLNRYWNKEVRNDLPKP+FLFS
Sbjct: 1   MHTLPAPLLVLLYLFIFLLSPLNVPAAADEDNPFTPKAFLNRYWNKEVRNDLPKPTFLFS 60

Query: 61  KVSPLTAVQSATFAKLAAENALSSRLPEFCSAANLLCFPDLSPSLAKHDRDSNFAVYSNR 120
           KVSPLTAVQSA FAKLAA+NALSSRLPEFCSAANLLCFPDLSPSLAKHD  SNFAVYSN+
Sbjct: 61  KVSPLTAVQSAAFAKLAAQNALSSRLPEFCSAANLLCFPDLSPSLAKHDSTSNFAVYSNK 120

Query: 121 NFTAYGTQRSGGIDSFKNYSNDDNVIVDSFRRYSHEALGHKDQFTTYATGTNVPDQSFNT 180
           NFT YGTQRSGGIDSFKNYSN DNV+VDSFRRYSHEALGH DQF+TY T TNVPDQSFNT
Sbjct: 121 NFTTYGTQRSGGIDSFKNYSNGDNVVVDSFRRYSHEALGHTDQFSTYGTDTNVPDQSFNT 180

Query: 181 YGSKAVGGDGKFTTYEESVNVPNLRFTSYSSDATGRSHTFKSYSENANAGDQSFANYGKR 240
           Y SKAVGG+G FTTYE+SVNVPNLRFT+YSSDATGRS +FKSYSENANAGDQSF NYGKR
Sbjct: 181 YASKAVGGNGGFTTYEQSVNVPNLRFTTYSSDATGRSQSFKSYSENANAGDQSFVNYGKR 240

Query: 241 GNGGPNEFAGYGTNANVVGSGFSSYGSEGNGANDTFTNYGNEQNNPQNNFRNYGEGGNGA 300
           GNGGPNEF GYGTNANVVGSGFSSYG+ GNGANDTFTNYGNEQNNPQNNFR+YG+GGNGA
Sbjct: 241 GNGGPNEFTGYGTNANVVGSGFSSYGANGNGANDTFTNYGNEQNNPQNNFRSYGDGGNGA 300

Query: 301 IESFSSYRDQANVGDDSFQSYSKNSNSAKVNFANYGKSFNEGTDKFSGYGQNANGQSVGF 360
           IESFSSYRDQANVGDDSFQSYSKNSNSAKVNFANYGKSFNEGTDKFSGYGQN NGQSVGF
Sbjct: 301 IESFSSYRDQANVGDDSFQSYSKNSNSAKVNFANYGKSFNEGTDKFSGYGQNGNGQSVGF 360

Query: 361 KIYGVNTTFKDYAKQGISFAKYSNVSSGGGKMSVSGSLAKRWVEPGKFFRESMLKKGTVM 420
           K YGVNTTFKDYAKQGISFAKYSNVSSGG KMSVS SLAKRWVEPGKFFRESML+KGTVM
Sbjct: 361 KTYGVNTTFKDYAKQGISFAKYSNVSSGGAKMSVSESLAKRWVEPGKFFRESMLRKGTVM 420

Query: 421 AMPDIRDKMPKRSFLPRSIVSKLPFSSSKVGVMKEIFQAGENSSMETMMTETLRECERAP 480
           AMPDIRDKMPKRSFLPRSI+SKLPFSSSK+ VMK+IF AG+NSSMETMMTETLR+CER P
Sbjct: 421 AMPDIRDKMPKRSFLPRSILSKLPFSSSKLEVMKQIFHAGDNSSMETMMTETLRDCERTP 480

Query: 481 SAGETKRCVGSVEDMIDFATSVLGRNVVVRTTENTKGSKGKVMIGAVKGINGGKVTQSVS 540
           SAGETKRCVGSVEDMIDFATSVLGRNVVVRTTENTKGSKG +MIGAVKGI GGKVT+SVS
Sbjct: 481 SAGETKRCVGSVEDMIDFATSVLGRNVVVRTTENTKGSKGNIMIGAVKGIEGGKVTESVS 540

Query: 541 CHQSLFPYLLYYCHSVPKVRVYEADLLDPSSKATINHGVAICHLDTSAWSPAHGAFIALG 600
           CHQSLFPYLLYYCHSVPKVRVYEA+LLDP+SKATINHGVAICHLDTSAWSPAHGAFIALG
Sbjct: 541 CHQSLFPYLLYYCHSVPKVRVYEAELLDPTSKATINHGVAICHLDTSAWSPAHGAFIALG 600

Query: 601 SAPGRVEVCHWIFENDMTWAVAD 624
           SAPGRVEVCHWIFENDMTW+VAD
Sbjct: 601 SAPGRVEVCHWIFENDMTWSVAD 623

BLAST of Lag0005327 vs. NCBI nr
Match: XP_022961767.1 (polygalacturonase 1 beta-like protein 3 [Cucurbita moschata])

HSP 1 Score: 1131.3 bits (2925), Expect = 0.0e+00
Identity = 568/623 (91.17%), Postives = 593/623 (95.18%), Query Frame = 0

Query: 1   MRRLPAPLVVPLSLFIFLLSALNVSAAGDEDNPFTPKAFLNRYWNKEVRNDLPKPSFLFS 60
           M  LPAPL+V L LFIFLLS LNV AA DEDNPFTPKAFLNRYWNKEVRNDLPKP+FLFS
Sbjct: 1   MHTLPAPLLVLLYLFIFLLSPLNVPAAADEDNPFTPKAFLNRYWNKEVRNDLPKPTFLFS 60

Query: 61  KVSPLTAVQSATFAKLAAENALSSRLPEFCSAANLLCFPDLSPSLAKHDRDSNFAVYSNR 120
           KVSPLTAVQSA FAKLAA+NALSSRLPEFCSAANLLCFPDLSPSLAKHD  SNFAVYSN+
Sbjct: 61  KVSPLTAVQSAAFAKLAAQNALSSRLPEFCSAANLLCFPDLSPSLAKHDSTSNFAVYSNK 120

Query: 121 NFTAYGTQRSGGIDSFKNYSNDDNVIVDSFRRYSHEALGHKDQFTTYATGTNVPDQSFNT 180
           NFT YGTQRSGGIDSFKNYSN DNV+VDSFRRYSHEALGH DQF+TY T TNVPDQSFNT
Sbjct: 121 NFTTYGTQRSGGIDSFKNYSNGDNVVVDSFRRYSHEALGHTDQFSTYGTDTNVPDQSFNT 180

Query: 181 YGSKAVGGDGKFTTYEESVNVPNLRFTSYSSDATGRSHTFKSYSENANAGDQSFANYGKR 240
           Y SKAVGG+G FTTYE+SVNVPNLRFT+YSSDATGRS +FKSYSENANAGDQSF NYGKR
Sbjct: 181 YASKAVGGNGGFTTYEQSVNVPNLRFTTYSSDATGRSQSFKSYSENANAGDQSFVNYGKR 240

Query: 241 GNGGPNEFAGYGTNANVVGSGFSSYGSEGNGANDTFTNYGNEQNNPQNNFRNYGEGGNGA 300
           GNGGPNEF GYGTNANV+GSGFSSYG+ GNGANDTFTNYGNEQNNPQNNFR+YG+GGNGA
Sbjct: 241 GNGGPNEFTGYGTNANVIGSGFSSYGANGNGANDTFTNYGNEQNNPQNNFRSYGDGGNGA 300

Query: 301 IESFSSYRDQANVGDDSFQSYSKNSNSAKVNFANYGKSFNEGTDKFSGYGQNANGQSVGF 360
           IESFSSYRDQANVGDDSFQSYSKNSNSAKVNFANYGKSFNEGTDKFSGYGQN NGQSVGF
Sbjct: 301 IESFSSYRDQANVGDDSFQSYSKNSNSAKVNFANYGKSFNEGTDKFSGYGQNGNGQSVGF 360

Query: 361 KIYGVNTTFKDYAKQGISFAKYSNVSSGGGKMSVSGSLAKRWVEPGKFFRESMLKKGTVM 420
           K YGVNTTFKDYAKQGISFAKYSNVSSGG KMSVS SLAKRWVEPGKFFRESML+KGTVM
Sbjct: 361 KTYGVNTTFKDYAKQGISFAKYSNVSSGGAKMSVSESLAKRWVEPGKFFRESMLRKGTVM 420

Query: 421 AMPDIRDKMPKRSFLPRSIVSKLPFSSSKVGVMKEIFQAGENSSMETMMTETLRECERAP 480
           AMPDIRDKMPKRSFLPRSI+SKLPFSSSK+ VMK+IF AG+NSSMETMMTETLR+CER P
Sbjct: 421 AMPDIRDKMPKRSFLPRSILSKLPFSSSKLEVMKQIFHAGDNSSMETMMTETLRDCERTP 480

Query: 481 SAGETKRCVGSVEDMIDFATSVLGRNVVVRTTENTKGSKGKVMIGAVKGINGGKVTQSVS 540
           SAGETKRCVGSVEDMIDFATSVLGRNVVVRTTENTKGSKG +MIGAVKGI GGKVT+SVS
Sbjct: 481 SAGETKRCVGSVEDMIDFATSVLGRNVVVRTTENTKGSKGNIMIGAVKGIEGGKVTESVS 540

Query: 541 CHQSLFPYLLYYCHSVPKVRVYEADLLDPSSKATINHGVAICHLDTSAWSPAHGAFIALG 600
           CHQSLFPYLLYYCHSVPKVRVYEA+LLDP+SKATINHGVAICHLDTSAWSPAHGAFIALG
Sbjct: 541 CHQSLFPYLLYYCHSVPKVRVYEAELLDPTSKATINHGVAICHLDTSAWSPAHGAFIALG 600

Query: 601 SAPGRVEVCHWIFENDMTWAVAD 624
           SAPGRVEVCHWIFENDMTW+VAD
Sbjct: 601 SAPGRVEVCHWIFENDMTWSVAD 623

BLAST of Lag0005327 vs. NCBI nr
Match: XP_004142981.1 (polygalacturonase 1 beta-like protein 3 [Cucumis sativus] >KGN62285.1 hypothetical protein Csa_018674 [Cucumis sativus])

HSP 1 Score: 1128.2 bits (2917), Expect = 0.0e+00
Identity = 561/623 (90.05%), Postives = 593/623 (95.18%), Query Frame = 0

Query: 1   MRRLPAPLVVPLSLFIFLLSALNVSAAGDEDNPFTPKAFLNRYWNKEVRNDLPKPSFLFS 60
           M RLP   VVPL LFIF LS+LNVS AGDED+PFTPKAFLNRYWNKEVRND PKPSFLFS
Sbjct: 1   MHRLPTLYVVPLYLFIFSLSSLNVSTAGDEDDPFTPKAFLNRYWNKEVRNDFPKPSFLFS 60

Query: 61  KVSPLTAVQSATFAKLAAENALSSRLPEFCSAANLLCFPDLSPSLAKHDRDSNFAVYSNR 120
           K SPLTAVQSA FAKLAAENALSSRLP+FCSAANLLCFPDLSPSLAKHDRDSNFAVYSN+
Sbjct: 61  KASPLTAVQSAAFAKLAAENALSSRLPDFCSAANLLCFPDLSPSLAKHDRDSNFAVYSNK 120

Query: 121 NFTAYGTQRSGGIDSFKNYSNDDNVIVDSFRRYSHEALGHKDQFTTYATGTNVPDQSFNT 180
           NFT YGTQRSGGIDSFKNYSN DNV+VDSFRRYSHEALGH D+F+ Y T TNVPDQSFNT
Sbjct: 121 NFTTYGTQRSGGIDSFKNYSNGDNVVVDSFRRYSHEALGHTDRFSVYGTDTNVPDQSFNT 180

Query: 181 YGSKAVGGDGKFTTYEESVNVPNLRFTSYSSDATGRSHTFKSYSENANAGDQSFANYGKR 240
           Y SKA+GGDGKFTTYE+SVNVPNLRFT+YSSDATGRS +FKSYSENANAGDQSF NYGKR
Sbjct: 181 YASKAIGGDGKFTTYEQSVNVPNLRFTTYSSDATGRSQSFKSYSENANAGDQSFVNYGKR 240

Query: 241 GNGGPNEFAGYGTNANVVGSGFSSYGSEGNGANDTFTNYGNEQNNPQNNFRNYGEGGNGA 300
           GNGGPNEF GYGT+ANV+GS FSSYG+EGNGANDTFTNYGN+QNNPQNNFR+YGEGGNGA
Sbjct: 241 GNGGPNEFTGYGTSANVIGSRFSSYGAEGNGANDTFTNYGNDQNNPQNNFRSYGEGGNGA 300

Query: 301 IESFSSYRDQANVGDDSFQSYSKNSNSAKVNFANYGKSFNEGTDKFSGYGQNANGQSVGF 360
           IESFSSYRDQANVGDDSFQSYSKNSNSAKVNFA+YGKSFNEGTDKFSGYGQN NGQSVGF
Sbjct: 301 IESFSSYRDQANVGDDSFQSYSKNSNSAKVNFASYGKSFNEGTDKFSGYGQNGNGQSVGF 360

Query: 361 KIYGVNTTFKDYAKQGISFAKYSNVSSGGGKMSVSGSLAKRWVEPGKFFRESMLKKGTVM 420
           K YGVNTTFKDYAKQGI+FAKYSNVSSGG KMSVSGSLAKRWVEPGKFFRESML+KG+VM
Sbjct: 361 KTYGVNTTFKDYAKQGINFAKYSNVSSGGAKMSVSGSLAKRWVEPGKFFRESMLRKGSVM 420

Query: 421 AMPDIRDKMPKRSFLPRSIVSKLPFSSSKVGVMKEIFQAGENSSMETMMTETLRECERAP 480
           AMPDIRDKMPKRSFLPRSI+SKLPFSSSK+GVMK+IF AG+NSSMETMMTET+RECER P
Sbjct: 421 AMPDIRDKMPKRSFLPRSILSKLPFSSSKLGVMKQIFHAGDNSSMETMMTETVRECERLP 480

Query: 481 SAGETKRCVGSVEDMIDFATSVLGRNVVVRTTENTKGSKGKVMIGAVKGINGGKVTQSVS 540
           SAGETKRCVGSVEDMIDFATSVLGRNVVV+TTENTKGSKG +MIG VKG+NGGKVT+SVS
Sbjct: 481 SAGETKRCVGSVEDMIDFATSVLGRNVVVQTTENTKGSKGNIMIGKVKGMNGGKVTESVS 540

Query: 541 CHQSLFPYLLYYCHSVPKVRVYEADLLDPSSKATINHGVAICHLDTSAWSPAHGAFIALG 600
           CHQSLFPYLLYYCHSVPKVRVYE DLLDP++KAT+NHGVAICHLDTSAWSPAHGAFIALG
Sbjct: 541 CHQSLFPYLLYYCHSVPKVRVYEVDLLDPATKATVNHGVAICHLDTSAWSPAHGAFIALG 600

Query: 601 SAPGRVEVCHWIFENDMTWAVAD 624
           SAPGRVEVCHWIFENDMTW VAD
Sbjct: 601 SAPGRVEVCHWIFENDMTWTVAD 623

BLAST of Lag0005327 vs. ExPASy Swiss-Prot
Match: P92990 (Polygalacturonase 1 beta-like protein 3 OS=Arabidopsis thaliana OX=3702 GN=PGL3 PE=2 SV=2)

HSP 1 Score: 800.4 bits (2066), Expect = 1.4e-230
Identity = 391/595 (65.71%), Postives = 472/595 (79.33%), Query Frame = 0

Query: 30  EDNPFTPKAFLNRYWNKEVRNDLPKPSFLFSKVSPLTAVQSATFAKLAAENALSSRLPEF 89
           E+NPFTPKA L RYWNKE+R   P+  FL SK SPL AV SATF+KLAA N+L +R P+F
Sbjct: 33  EENPFTPKASLVRYWNKEIRGQSPRSEFLISKASPLNAVDSATFSKLAAANSLPTRFPDF 92

Query: 90  CSAANLLCFPDLSPSLAKHDRDSNFAVYSNRNFTAYGTQRSGGIDSFKNYSNDDNVIVDS 149
           CSAANL CFPDL  SL KHD D  F+VY  +NFT YG  R+GG DSFKNYS D NV+ DS
Sbjct: 93  CSAANLFCFPDLGASLEKHDDDVKFSVYDQKNFTNYGNARAGGADSFKNYSKDGNVVTDS 152

Query: 150 FRRYSHEALGHKDQFTTYATGTNVPDQSFNTYGSKAVGGDGKFTTYEESVNVPNLRFTSY 209
           FRRYS  A GH D+FT Y   +NV ++ FN+YG+   GG G FT Y+ +VN P  RFT+Y
Sbjct: 153 FRRYSRNAAGHDDKFTVYGENSNVVEEGFNSYGTFGTGGAGDFTNYQNNVNNPTSRFTAY 212

Query: 210 SSDATGRSHTFKSYSENANAGD-QSFANYGKRGNGGPNEFAGYGTNANVVGSGFSSYGSE 269
           S    GRS TFK+Y+  ANAG+ QSF +YGK GNG PNEF  YG ++NV+GSGFS+YG  
Sbjct: 213 SDGGNGRSQTFKTYTHEANAGNGQSFTSYGKNGNGVPNEFTSYGVSSNVIGSGFSNYGES 272

Query: 270 GNGANDTFTNYGNEQNNPQNNFRNYGEGGNGAIESFSSYRDQANVGDDSFQSYSKNSNSA 329
           GN ANDTFT+YG++ N PQNNF NYG  GN A+++F++YRD+ANVGDDSF SY+K+SNS 
Sbjct: 273 GNAANDTFTSYGSDGNVPQNNFNNYGASGNAAVDTFANYRDKANVGDDSFSSYAKDSNSE 332

Query: 330 KVNFANYGKSFNEGTDKFSGYGQNANGQSVGFKIYGVNTTFKDYAKQGISFAKYSNVSSG 389
           KVNF NYG+SFN G++ F+GYG+ A G  + FK Y  N+TFKDYAK+G++FAKY NVS+ 
Sbjct: 333 KVNFVNYGQSFNPGSETFTGYGKGAEGSKLSFKTYTPNSTFKDYAKKGVAFAKY-NVSTT 392

Query: 390 GGKMSVSGSLAKRWVEPGKFFRESMLKKGTVMAMPDIRDKMPKRSFLPRSIVSKLPFSSS 449
                  G    +W+EPGKFFRES LK+GTV+ MPDI+DKMPKRSFLPRSI++KLPFS+S
Sbjct: 393 TANTVGDGKTVNKWIEPGKFFRESSLKEGTVIPMPDIKDKMPKRSFLPRSIITKLPFSTS 452

Query: 450 KVGVMKEIFQAGENSSMETMMTETLRECERAPSAGETKRCVGSVEDMIDFATSVLGRNVV 509
           K+G +K IF A ENS+M  ++T+ + ECER PS GETKRCVGS EDMIDFATSVLGR+VV
Sbjct: 453 KLGEIKRIFHAVENSTMGGIITDAVTECERPPSVGETKRCVGSAEDMIDFATSVLGRSVV 512

Query: 510 VRTTENTKGSKGKVMIGAVKGINGGKVTQSVSCHQSLFPYLLYYCHSVPKVRVYEADLLD 569
           +RTTEN  GSK KV+IG V GINGGK+T++VSCHQSL+PYLLYYCHSVPKVRVYEADLL+
Sbjct: 513 LRTTENVAGSKEKVVIGKVNGINGGKLTKAVSCHQSLYPYLLYYCHSVPKVRVYEADLLE 572

Query: 570 PSSKATINHGVAICHLDTSAWSPAHGAFIALGSAPGRVEVCHWIFENDMTWAVAD 624
            +SK  INHG+AICH+DTS+W P+HGAF+ALGS PGR+EVCHWIFENDM WA+AD
Sbjct: 573 LNSKKKINHGIAICHMDTSSWGPSHGAFLALGSKPGRIEVCHWIFENDMNWAIAD 626

BLAST of Lag0005327 vs. ExPASy Swiss-Prot
Match: P92982 (Polygalacturonase 1 beta-like protein 1 OS=Arabidopsis thaliana OX=3702 GN=PGL1 PE=2 SV=2)

HSP 1 Score: 789.6 bits (2038), Expect = 2.5e-227
Identity = 394/624 (63.14%), Postives = 473/624 (75.80%), Query Frame = 0

Query: 15  FIFLLSALN-----VSAAGD---------EDNPFTPKAFLNRYWNKEVRNDLPKPSFLFS 74
           F+FLL  L+     V A G+         E NPFTPKA L RYWNK++ +  P  +FL S
Sbjct: 5   FVFLLPFLSRLYHVVIAGGERLTNGGFSPEINPFTPKASLVRYWNKQIHHQSPGSAFLLS 64

Query: 75  KVSPLTAVQSATFAKLAAENALSSRLPEFCSAANLLCFPDLSPSLAKHDRDSNFAVYSNR 134
           K SPLTAV SATFAKLA+ENAL  RLP+FCSAANL CFPDL P+L KH  D  F+VY  R
Sbjct: 65  KASPLTAVASATFAKLASENALPDRLPDFCSAANLFCFPDLGPTLEKHSNDVKFSVYDQR 124

Query: 135 NFTAYGTQRSGGIDSFKNYSNDDNVIVDSFRRYSHEALGHKDQFTTYATGTNVPDQSFNT 194
           NFT YGT   GG DSFK YS + N + DSFRRYS +A GH+D+FT YA  +NV ++ FN+
Sbjct: 125 NFTNYGTIHPGGADSFKKYSQNGNAVTDSFRRYSRDAAGHEDKFTGYADNSNVVEEKFNS 184

Query: 195 YGSKAVGGDGKFTTYEESVNVPNLRFTSYSSDATGRSHTFKSYSENANAG-DQSFANYGK 254
           YG+ A GG G F  Y+  VN P  RF +YS +  GR+ TFK+Y+  AN+G  QSF +YGK
Sbjct: 185 YGTSATGGSGDFKNYQSGVNNPTTRFKAYSDEGNGRAQTFKTYTHEANSGPGQSFTSYGK 244

Query: 255 RGNGGPNEFAGYGTNANVVGSGFSSYGSEGNGANDTFTNYGNEQNNPQNNFRNYGEGGNG 314
            GNG PNEF+ YG ++NV+GS FS+YG  GN ANDTF  YG + N PQNNF++YG  GN 
Sbjct: 245 NGNGAPNEFSSYGVSSNVIGSDFSNYGENGNAANDTFKGYGGDGNVPQNNFKSYGASGNA 304

Query: 315 AIESFSSYRDQANVGDDSFQSYSKNSNSAKVNFANYGKSFNEGTDKFSGYGQNANGQSVG 374
           A+++F +YRD ANVGDDSF SY+KNSN  KV+F NYGKS N G++ FSGYG+ A G  + 
Sbjct: 305 AVDTFLNYRDNANVGDDSFSSYAKNSNFEKVDFVNYGKSINPGSESFSGYGKGAEGNKID 364

Query: 375 FKIYGVNTTFKDYAKQGISFAKYSNVSSGGGKMSVSGSLAKRWVEPGKFFRESMLKKGTV 434
           FK Y  N+TFKDY K G+ FAKY+  S GGGK         +WVEPGKFFRESMLK+GT+
Sbjct: 365 FKTYTQNSTFKDYTKTGVEFAKYNRSSLGGGK------TVNKWVEPGKFFRESMLKEGTL 424

Query: 435 MAMPDIRDKMPKRSFLPRSIVSKLPFSSSKVGVMKEIFQAGENSSMETMMTETLRECERA 494
           + MPDI+DKMPKRSFLPRSIVSKLPFS+SK+  +K +F A +NS+ME ++T+ +RECER 
Sbjct: 425 IWMPDIKDKMPKRSFLPRSIVSKLPFSTSKIAEIKRVFHANDNSTMEGIITDAVRECERP 484

Query: 495 PSAGETKRCVGSVEDMIDFATSVLGRNVVVRTTENTKGSKGKVMIGAVKGINGGKVTQSV 554
           P+  ETKRCVGS EDMIDFATSVLGR+VV+RTTE+  GSK KVMIG V GINGG+VT+SV
Sbjct: 485 PTVSETKRCVGSAEDMIDFATSVLGRSVVLRTTESVAGSKEKVMIGKVNGINGGRVTKSV 544

Query: 555 SCHQSLFPYLLYYCHSVPKVRVYEADLLDPSSKATINHGVAICHLDTSAWSPAHGAFIAL 614
           SCHQSL+PYLLYYCHSVPKVRVYE+DLLDP SKA INHG+AICH+DTSAW   HGAF+ L
Sbjct: 545 SCHQSLYPYLLYYCHSVPKVRVYESDLLDPKSKAKINHGIAICHMDTSAWGANHGAFMLL 604

Query: 615 GSAPGRVEVCHWIFENDMTWAVAD 624
           GS PG++EVCHWIFENDM WA+AD
Sbjct: 605 GSRPGQIEVCHWIFENDMNWAIAD 622

BLAST of Lag0005327 vs. ExPASy Swiss-Prot
Match: O80760 (Polygalacturonase 1 beta-like protein 2 OS=Arabidopsis thaliana OX=3702 GN=PGL2 PE=2 SV=1)

HSP 1 Score: 759.2 bits (1959), Expect = 3.6e-218
Identity = 385/619 (62.20%), Postives = 452/619 (73.02%), Query Frame = 0

Query: 15  FIFLLSALNVSAAG---------DEDNPFTPKAFLNRYWNKEVRNDLPKPSFLFSKVSPL 74
           F  + S+ NV  AG           +NPFTPKA L RYWN  +  D PKPSF  SK SPL
Sbjct: 12  FFCIFSSSNVHFAGAKQTAGNITPSENPFTPKASLIRYWNNHINGDSPKPSFFLSKASPL 71

Query: 75  TAVQSATFAKLAAENALSSRLPEFCSAANLLCFPDLSP-SLAKHDRDSNFAVYSNRNFTA 134
           TAV S  FA LA+ +AL++   +FCSAA L CFP+L+  SL KH  D NFA YS +NFT 
Sbjct: 72  TAVDSTRFASLASNHALNTHHSDFCSAAKLFCFPELAAHSLEKHGDDVNFAAYSGKNFTN 131

Query: 135 YGTQRSGGIDSFKNYSNDDNVIVDSFRRYSHEALGHKDQFTTYATGTNVPDQSFNTYGSK 194
           YG+ R  G DSFKNYS  DN+ VDSFRRYS  + GH D FT YA   NV DQSF TY + 
Sbjct: 132 YGSDRLSGADSFKNYSGGDNIAVDSFRRYSRNSAGHDDGFTNYAGEVNVADQSFTTYATG 191

Query: 195 AVGGDGKFTTYEESVNVPNLRFTSYSSDATGRSHTFKSYSENANAGDQSFANYGKRGNGG 254
             GG G+FT Y    N PN RFTSYS  A GRS TF +YSEN N G QSF +Y K GNG 
Sbjct: 192 TTGGSGEFTNYNTDANEPNGRFTSYSDKANGRSQTFTTYSENGNTGYQSFTSYSKNGNGA 251

Query: 255 PNEFAGYGTNANVVGSGFSSYGSEGNGANDTFTNYGNEQNNPQNNFRNYGEGGNGAIESF 314
           PNEF+GYGT +NVV +GF+ YG   NGAND+FT+YG   N P N F+ YG+GGNGA+  F
Sbjct: 252 PNEFSGYGTGSNVVNTGFTKYGESANGANDSFTSYGENGNVPVNEFKGYGDGGNGAVYGF 311

Query: 315 SSYRDQANVGDDSFQSYSKNSNSAKVNFANYGKSFNEGTDKFSGYGQNANGQSVGFKIYG 374
            +YRDQ+N+G DSF SY+KNSN+ KVNF NYGKSFN G+D F+GYGQ+  G +V FK YG
Sbjct: 312 KNYRDQSNIGVDSFSSYAKNSNNEKVNFVNYGKSFNLGSDNFTGYGQDNVGGNVSFKTYG 371

Query: 375 VNTTFKDYAKQGISFAKYSNVSSGGGKMSVSGSLAKRWVEPGKFFRESMLKKGTVMAMPD 434
              +FK Y K G+ FA+YSN       +S +G    +WVE GKFFRE+MLK+GT+M MPD
Sbjct: 372 QGQSFKVYTKDGVVFARYSN------NVSSNGKTVNKWVEEGKFFREAMLKEGTLMQMPD 431

Query: 435 IRDKMPKRSFLPRSIVSKLPFSSSKVGVMKEIFQAGENSSMETMMTETLRECERAPSAGE 494
           I+DKMPKR+FLPR+IV  LPFSSS +G +  +F AGENSSM  +++  + ECER  S GE
Sbjct: 432 IKDKMPKRTFLPRNIVKNLPFSSSTIGEIWRVFGAGENSSMAGIISSAVSECERPASHGE 491

Query: 495 TKRCVGSVEDMIDFATSVLGRNVVVRTTENTKGSKGKVMIGAVKGINGGKVTQSVSCHQS 554
           TKRCVGS EDMIDFATSVLGR VVVRTTEN  GSK KV+IG V GINGG VT++VSCHQS
Sbjct: 492 TKRCVGSAEDMIDFATSVLGRGVVVRTTENVVGSKKKVVIGKVNGINGGDVTRAVSCHQS 551

Query: 555 LFPYLLYYCHSVPKVRVYEADLLDPSSKATINHGVAICHLDTSAWSPAHGAFIALGSAPG 614
           L+PYLLYYCHSVP+VRVYE DLLDP S   INHGVAICH+DTSAWSP+HGAF+ALGS PG
Sbjct: 552 LYPYLLYYCHSVPRVRVYETDLLDPKSLEKINHGVAICHIDTSAWSPSHGAFLALGSGPG 611

Query: 615 RVEVCHWIFENDMTWAVAD 624
           ++EVCHWIFENDMTW + D
Sbjct: 612 QIEVCHWIFENDMTWNIID 624

BLAST of Lag0005327 vs. ExPASy Swiss-Prot
Match: Q40161 (Polygalacturonase-1 non-catalytic subunit beta OS=Solanum lycopersicum OX=4081 GN=GP1 PE=1 SV=1)

HSP 1 Score: 756.9 bits (1953), Expect = 1.8e-217
Identity = 383/620 (61.77%), Postives = 465/620 (75.00%), Query Frame = 0

Query: 12  LSLFIFLLSALNVSAAGDED--NPFTPKAFLNRYWNKEVRNDLPKPSFLFSKVSPLTAVQ 71
           L L +F L + NV   GD +  NPFTPK +L RYW K++ NDLPKP FL +K SPL A Q
Sbjct: 12  LLLLLFSLPSFNVVVGGDGESGNPFTPKGYLIRYWKKQISNDLPKPWFLLNKASPLNAAQ 71

Query: 72  SATFAKLAA-ENALSSRLPEFCSAANLLCFPDLSPSLAKHDRDSNFAVYSNRNFTAYGTQ 131
            AT+ KL A +NAL+++L  FCS+ANL+C PDLSPSL KH  D +FA YS++NFT YGT 
Sbjct: 72  YATYTKLVADQNALTTQLHTFCSSANLMCAPDLSPSLEKHSGDIHFATYSDKNFTNYGTN 131

Query: 132 RSG-GIDSFKNYSNDDNVIVDSFRRYSHEALGHKDQFTTYATGTNVPDQSFNTYGSKAVG 191
             G G+++FKNYS  +N+ V+SFRRY   +    ++F  YA+  NV DQSFN+Y +   G
Sbjct: 132 EPGIGVNTFKNYSEGENIPVNSFRRYGRGS-PRDNKFDNYASDGNVIDQSFNSYSTSTAG 191

Query: 192 GDGKFTTYEESVNVPNLRFTSYSSDATGRSHTFKSYSENANAGDQSFANYGKRGNGGPNE 251
           G GKFT Y  + N PNL FTSYS   TG    F  YS+ ANAGDQ F +YGK GNG   E
Sbjct: 192 GSGKFTNYAANANDPNLHFTSYSDQGTGGVQKFTIYSQEANAGDQYFKSYGKNGNGANGE 251

Query: 252 FAGYGTNANVVGSGFSSYGSEGNGANDTFTNYGNEQNNPQNNFRNYGEGGNGAIESFSSY 311
           F  YG + NV+GS F++YG   NG +  FT+YG   N P+N+F NYG GGNG  E+F+SY
Sbjct: 252 FVSYGNDTNVIGSTFTNYGQTANGGDQKFTSYGFNGNVPENHFTNYGAGGNGPSETFNSY 311

Query: 312 RDQANVGDDSFQSYSKNSNSAKVNFANYGKSFNEGTDKFSGYGQNANGQSVGFKIYGVNT 371
           RDQ+NVGDD+F +Y K++N  + NF NYG+SFNEGTD F+ YG+  N   + FK YGVN 
Sbjct: 312 RDQSNVGDDTFTTYVKDANGGEANFTNYGQSFNEGTDVFTTYGKGGNDPHINFKTYGVNN 371

Query: 372 TFKDYAKQGISFAKYSNVSSG--GGKMSVSG--SLAKRWVEPGKFFRESMLKKGTVMAMP 431
           TFKDY K   +F+ Y N +S      M V+G   +  RWVEPGKFFRE MLK GT+M MP
Sbjct: 372 TFKDYVKDTATFSNYHNKTSQVLASLMEVNGGKKVNNRWVEPGKFFREKMLKSGTIMPMP 431

Query: 432 DIRDKMPKRSFLPRSIVSKLPFSSSKVGVMKEIFQAGENSSMETMMTETLRECERAPSAG 491
           DI+DKMPKRSFLPR I SKLPFS+SK+  +K+IF AG+ S +E M+ + L ECERAPSAG
Sbjct: 432 DIKDKMPKRSFLPRVIASKLPFSTSKIAELKKIFHAGDESQVEKMIGDALSECERAPSAG 491

Query: 492 ETKRCVGSVEDMIDFATSVLGRNVVVRTTENTKGSKGKVMIGAVKGINGGKVTQSVSCHQ 551
           ETKRCV S EDMIDFATSVLGRNVVVRTTE+TKGS G +MIG+VKGINGGKVT+SVSCHQ
Sbjct: 492 ETKRCVNSAEDMIDFATSVLGRNVVVRTTEDTKGSNGNIMIGSVKGINGGKVTKSVSCHQ 551

Query: 552 SLFPYLLYYCHSVPKVRVYEADLLDPSSKATINHGVAICHLDTSAWSPAHGAFIALGSAP 611
           +L+PYLLYYCHSVPKVRVYEAD+LDP+SK  INHGVAICH+DTS+W P+HGAF+ALGS P
Sbjct: 552 TLYPYLLYYCHSVPKVRVYEADILDPNSKVKINHGVAICHVDTSSWGPSHGAFVALGSGP 611

Query: 612 GRVEVCHWIFENDMTWAVAD 624
           G++EVCHWIFENDMTWA+AD
Sbjct: 612 GKIEVCHWIFENDMTWAIAD 630

BLAST of Lag0005327 vs. ExPASy Swiss-Prot
Match: P93218 (Polygalacturonase non-catalytic subunit AroGP3 OS=Solanum lycopersicum OX=4081 GN=GP3 PE=3 SV=1)

HSP 1 Score: 738.0 bits (1904), Expect = 8.6e-212
Identity = 379/627 (60.45%), Postives = 458/627 (73.05%), Query Frame = 0

Query: 12  LSLFIFLLSALNVSAAGDED---NPFTPKAFLNRYWNKEVRNDLPKPSFLFSKVSPLTAV 71
           L L +F LS+L+V  A D D   NPFTPK +L RYWNK V NDLPKP FL +K SPL A 
Sbjct: 12  LLLLLFTLSSLDVVVAKDGDESGNPFTPKGYLIRYWNKHVSNDLPKPWFLLNKASPLNAA 71

Query: 72  QSATFAKLAA-ENALSSRLPEFCSAANLLCFPDLSPSLAKHDRDSNFAVYSNRNFTAYGT 131
           Q AT+ KL A +NA ++ L  FCS+ANL+C  DL PSL KH  D +F  Y ++NFT YGT
Sbjct: 72  QYATYTKLVADQNAFTTHLQSFCSSANLMCALDLLPSLEKHKGDVHFVSYGDKNFTNYGT 131

Query: 132 QRSG-GIDSFKNYSNDDNVIVDSFRRYSHEALGHKDQFTTY-ATGTNVPDQSFNTYGSKA 191
           + SG G +SFKNY+ ++N  V+SFRRY  ++  H +QF  Y A G N P QSFN+Y +  
Sbjct: 132 KESGLGFNSFKNYTEEENFPVNSFRRYGRDS-PHDNQFDNYGAPGGNTPVQSFNSYSTNT 191

Query: 192 VGGDGKFTTYEESVNVPNLRFTSYSSDATGRSHTFKSYSENANAGDQSFANYGKRGNGGP 251
            GG G+FT Y    NVP+L FTSYS   TG    FKSY  + NAG QSF +YGK GNG  
Sbjct: 192 PGGSGQFTNYAPGSNVPDLHFTSYSDHGTGGEQDFKSYGNDGNAGQQSFKSYGKDGNGAD 251

Query: 252 NEFAGYGTNANVVGSGFSSYGSEGNGANDTFTNYGNEQNNPQNNFRNYGEGGNGAIESFS 311
           ++F  YG N NV GS F++YG   NG N  FT+YG   NNP ++F NYG GGNG  E+F+
Sbjct: 252 SQFTTYGNNTNVDGSDFTNYGEAANGENQNFTSYGFNGNNPASSFNNYGVGGNGPSEAFN 311

Query: 312 SYRDQANVGDDSFQSYSKNSNSAKVNFANYGKSFNEGTDKFSGYGQNANGQSVGFKIYGV 371
           SYRDQ+NVGDD+F +Y K++N  + NF NYG+SFNEGTD F+ YG+  N   + FK YGV
Sbjct: 312 SYRDQSNVGDDTFTTYVKDANGGEANFTNYGQSFNEGTDVFTTYGKGGNDPHINFKTYGV 371

Query: 372 NTTFKDYAKQGISFAKYSNVSS---------GGGKMSVSGSLAKRWVEPGKFFRESMLKK 431
           N TFKDY K   +F+ Y N +S          GGK      +  RW+EPGKFFRE MLK 
Sbjct: 372 NNTFKDYVKDTATFSNYHNKTSQDLASLSEVNGGK-----KVNNRWIEPGKFFREKMLKS 431

Query: 432 GTVMAMPDIRDKMPKRSFLPRSIVSKLPFSSSKVGVMKEIFQAGENSSMETMMTETLREC 491
           GT+M MPDI+DKMPKRSFLPR+I +KLPFS+SK+  +K+IF A  +S +  M+ + L EC
Sbjct: 432 GTIMPMPDIKDKMPKRSFLPRAIAAKLPFSTSKIDELKKIFHAANDSQVAKMIGDALSEC 491

Query: 492 ERAPSAGETKRCVGSVEDMIDFATSVLGRNVVVRTTENTKGSKGKVMIGAVKGINGGKVT 551
           ERAPS GETK+CV S EDMIDFATSVLGRNVVVRTTENT GSKG +MIG++KGINGGKVT
Sbjct: 492 ERAPSPGETKQCVNSAEDMIDFATSVLGRNVVVRTTENTNGSKGNIMIGSIKGINGGKVT 551

Query: 552 QSVSCHQSLFPYLLYYCHSVPKVRVYEADLLDPSSKATINHGVAICHLDTSAWSPAHGAF 611
           +SVSCHQ+L+P LLYYCHSVPKVRVYEAD+LDP+SKA INHGVAICH+DTS+W P HGAF
Sbjct: 552 KSVSCHQTLYPSLLYYCHSVPKVRVYEADILDPNSKAKINHGVAICHVDTSSWGPRHGAF 611

Query: 612 IALGSAPGRVEVCHWIFENDMTWAVAD 624
           +ALGS PG++EVCHWIFENDMTWA AD
Sbjct: 612 VALGSGPGKIEVCHWIFENDMTWATAD 632

BLAST of Lag0005327 vs. ExPASy TrEMBL
Match: A0A5A7V5U8 (Polygalacturonase 1 beta-like protein 3 OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold501G002300 PE=4 SV=1)

HSP 1 Score: 1132.5 bits (2928), Expect = 0.0e+00
Identity = 565/623 (90.69%), Postives = 593/623 (95.18%), Query Frame = 0

Query: 1   MRRLPAPLVVPLSLFIFLLSALNVSAAGDEDNPFTPKAFLNRYWNKEVRNDLPKPSFLFS 60
           M RLP   VVPL LFIF LSALNV A GDEDNPFTPKAFLNRYWNKEVRND+PKPSFLFS
Sbjct: 1   MHRLPTLHVVPLYLFIFSLSALNVPAVGDEDNPFTPKAFLNRYWNKEVRNDIPKPSFLFS 60

Query: 61  KVSPLTAVQSATFAKLAAENALSSRLPEFCSAANLLCFPDLSPSLAKHDRDSNFAVYSNR 120
           K SPLTAVQSA FAKLAAENALSSRLP+FCSAANLLCFPDLSPSLAKHDRDSNFAVYSN+
Sbjct: 61  KASPLTAVQSAAFAKLAAENALSSRLPDFCSAANLLCFPDLSPSLAKHDRDSNFAVYSNK 120

Query: 121 NFTAYGTQRSGGIDSFKNYSNDDNVIVDSFRRYSHEALGHKDQFTTYATGTNVPDQSFNT 180
           NFT YGTQRSGGIDSFKNYSN DNV+VDSFRRYSHEALGH D+F+TY T TNVPDQSFNT
Sbjct: 121 NFTTYGTQRSGGIDSFKNYSNGDNVVVDSFRRYSHEALGHTDRFSTYGTETNVPDQSFNT 180

Query: 181 YGSKAVGGDGKFTTYEESVNVPNLRFTSYSSDATGRSHTFKSYSENANAGDQSFANYGKR 240
           Y SKA+GGDGKFTTYE+SVNVPNLRFT+YSSDATGRS  FKSYSENANAGDQSF NYGKR
Sbjct: 181 YASKAIGGDGKFTTYEQSVNVPNLRFTTYSSDATGRSQAFKSYSENANAGDQSFVNYGKR 240

Query: 241 GNGGPNEFAGYGTNANVVGSGFSSYGSEGNGANDTFTNYGNEQNNPQNNFRNYGEGGNGA 300
           GNGGPNEF GYGT+ANVVGS FSSYG++GNGANDTFTNYGN+QNNPQNNFR+YGEGGNGA
Sbjct: 241 GNGGPNEFTGYGTSANVVGSRFSSYGTDGNGANDTFTNYGNDQNNPQNNFRSYGEGGNGA 300

Query: 301 IESFSSYRDQANVGDDSFQSYSKNSNSAKVNFANYGKSFNEGTDKFSGYGQNANGQSVGF 360
           IESFSSYRDQANVGDDSFQSYSKNSNSAKVNFA+YGKSFNEGTDKFSGYGQN NGQSVGF
Sbjct: 301 IESFSSYRDQANVGDDSFQSYSKNSNSAKVNFASYGKSFNEGTDKFSGYGQNGNGQSVGF 360

Query: 361 KIYGVNTTFKDYAKQGISFAKYSNVSSGGGKMSVSGSLAKRWVEPGKFFRESMLKKGTVM 420
           K+YGVNTTFKDYAKQGI+FAKYSNVSSGG K SVSGSLAKRWVEPGKFFRESML+KG+VM
Sbjct: 361 KVYGVNTTFKDYAKQGINFAKYSNVSSGGAKTSVSGSLAKRWVEPGKFFRESMLRKGSVM 420

Query: 421 AMPDIRDKMPKRSFLPRSIVSKLPFSSSKVGVMKEIFQAGENSSMETMMTETLRECERAP 480
           AMPDIRDKMPKRSFLPRSI+SKLPFSSSK+GVMK+IF AG+NSSMETMMTET+RECER P
Sbjct: 421 AMPDIRDKMPKRSFLPRSILSKLPFSSSKLGVMKQIFHAGDNSSMETMMTETVRECERVP 480

Query: 481 SAGETKRCVGSVEDMIDFATSVLGRNVVVRTTENTKGSKGKVMIGAVKGINGGKVTQSVS 540
           SAGETKRCVGSVEDMIDFATSVLGRNVVVRTTENTKGSKG +MIG VKG+NGGKVT+SVS
Sbjct: 481 SAGETKRCVGSVEDMIDFATSVLGRNVVVRTTENTKGSKGNIMIGKVKGMNGGKVTESVS 540

Query: 541 CHQSLFPYLLYYCHSVPKVRVYEADLLDPSSKATINHGVAICHLDTSAWSPAHGAFIALG 600
           CHQSLFPYLLYYCHSVPKVRVYE DLLDP+SKATINHGVAICHLDTSAWSPAHGAFIALG
Sbjct: 541 CHQSLFPYLLYYCHSVPKVRVYEVDLLDPASKATINHGVAICHLDTSAWSPAHGAFIALG 600

Query: 601 SAPGRVEVCHWIFENDMTWAVAD 624
           SAPGRVEVCHWIFENDMTW VAD
Sbjct: 601 SAPGRVEVCHWIFENDMTWTVAD 623

BLAST of Lag0005327 vs. ExPASy TrEMBL
Match: A0A1S3B9L4 (polygalacturonase 1 beta-like protein 3 OS=Cucumis melo OX=3656 GN=LOC103487686 PE=4 SV=1)

HSP 1 Score: 1132.5 bits (2928), Expect = 0.0e+00
Identity = 565/623 (90.69%), Postives = 593/623 (95.18%), Query Frame = 0

Query: 1   MRRLPAPLVVPLSLFIFLLSALNVSAAGDEDNPFTPKAFLNRYWNKEVRNDLPKPSFLFS 60
           M RLP   VVPL LFIF LSALNV A GDEDNPFTPKAFLNRYWNKEVRND+PKPSFLFS
Sbjct: 1   MHRLPTLHVVPLYLFIFSLSALNVPAVGDEDNPFTPKAFLNRYWNKEVRNDIPKPSFLFS 60

Query: 61  KVSPLTAVQSATFAKLAAENALSSRLPEFCSAANLLCFPDLSPSLAKHDRDSNFAVYSNR 120
           K SPLTAVQSA FAKLAAENALSSRLP+FCSAANLLCFPDLSPSLAKHDRDSNFAVYSN+
Sbjct: 61  KASPLTAVQSAAFAKLAAENALSSRLPDFCSAANLLCFPDLSPSLAKHDRDSNFAVYSNK 120

Query: 121 NFTAYGTQRSGGIDSFKNYSNDDNVIVDSFRRYSHEALGHKDQFTTYATGTNVPDQSFNT 180
           NFT YGTQRSGGIDSFKNYSN DNV+VDSFRRYSHEALGH D+F+TY T TNVPDQSFNT
Sbjct: 121 NFTTYGTQRSGGIDSFKNYSNGDNVVVDSFRRYSHEALGHTDRFSTYGTETNVPDQSFNT 180

Query: 181 YGSKAVGGDGKFTTYEESVNVPNLRFTSYSSDATGRSHTFKSYSENANAGDQSFANYGKR 240
           Y SKA+GGDGKFTTYE+SVNVPNLRFT+YSSDATGRS  FKSYSENANAGDQSF NYGKR
Sbjct: 181 YASKAIGGDGKFTTYEQSVNVPNLRFTTYSSDATGRSQAFKSYSENANAGDQSFVNYGKR 240

Query: 241 GNGGPNEFAGYGTNANVVGSGFSSYGSEGNGANDTFTNYGNEQNNPQNNFRNYGEGGNGA 300
           GNGGPNEF GYGT+ANVVGS FSSYG++GNGANDTFTNYGN+QNNPQNNFR+YGEGGNGA
Sbjct: 241 GNGGPNEFTGYGTSANVVGSRFSSYGTDGNGANDTFTNYGNDQNNPQNNFRSYGEGGNGA 300

Query: 301 IESFSSYRDQANVGDDSFQSYSKNSNSAKVNFANYGKSFNEGTDKFSGYGQNANGQSVGF 360
           IESFSSYRDQANVGDDSFQSYSKNSNSAKVNFA+YGKSFNEGTDKFSGYGQN NGQSVGF
Sbjct: 301 IESFSSYRDQANVGDDSFQSYSKNSNSAKVNFASYGKSFNEGTDKFSGYGQNGNGQSVGF 360

Query: 361 KIYGVNTTFKDYAKQGISFAKYSNVSSGGGKMSVSGSLAKRWVEPGKFFRESMLKKGTVM 420
           K+YGVNTTFKDYAKQGI+FAKYSNVSSGG K SVSGSLAKRWVEPGKFFRESML+KG+VM
Sbjct: 361 KVYGVNTTFKDYAKQGINFAKYSNVSSGGAKTSVSGSLAKRWVEPGKFFRESMLRKGSVM 420

Query: 421 AMPDIRDKMPKRSFLPRSIVSKLPFSSSKVGVMKEIFQAGENSSMETMMTETLRECERAP 480
           AMPDIRDKMPKRSFLPRSI+SKLPFSSSK+GVMK+IF AG+NSSMETMMTET+RECER P
Sbjct: 421 AMPDIRDKMPKRSFLPRSILSKLPFSSSKLGVMKQIFHAGDNSSMETMMTETVRECERVP 480

Query: 481 SAGETKRCVGSVEDMIDFATSVLGRNVVVRTTENTKGSKGKVMIGAVKGINGGKVTQSVS 540
           SAGETKRCVGSVEDMIDFATSVLGRNVVVRTTENTKGSKG +MIG VKG+NGGKVT+SVS
Sbjct: 481 SAGETKRCVGSVEDMIDFATSVLGRNVVVRTTENTKGSKGNIMIGKVKGMNGGKVTESVS 540

Query: 541 CHQSLFPYLLYYCHSVPKVRVYEADLLDPSSKATINHGVAICHLDTSAWSPAHGAFIALG 600
           CHQSLFPYLLYYCHSVPKVRVYE DLLDP+SKATINHGVAICHLDTSAWSPAHGAFIALG
Sbjct: 541 CHQSLFPYLLYYCHSVPKVRVYEVDLLDPASKATINHGVAICHLDTSAWSPAHGAFIALG 600

Query: 601 SAPGRVEVCHWIFENDMTWAVAD 624
           SAPGRVEVCHWIFENDMTW VAD
Sbjct: 601 SAPGRVEVCHWIFENDMTWTVAD 623

BLAST of Lag0005327 vs. ExPASy TrEMBL
Match: A0A6J1HB03 (polygalacturonase 1 beta-like protein 3 OS=Cucurbita moschata OX=3662 GN=LOC111462438 PE=4 SV=1)

HSP 1 Score: 1131.3 bits (2925), Expect = 0.0e+00
Identity = 568/623 (91.17%), Postives = 593/623 (95.18%), Query Frame = 0

Query: 1   MRRLPAPLVVPLSLFIFLLSALNVSAAGDEDNPFTPKAFLNRYWNKEVRNDLPKPSFLFS 60
           M  LPAPL+V L LFIFLLS LNV AA DEDNPFTPKAFLNRYWNKEVRNDLPKP+FLFS
Sbjct: 1   MHTLPAPLLVLLYLFIFLLSPLNVPAAADEDNPFTPKAFLNRYWNKEVRNDLPKPTFLFS 60

Query: 61  KVSPLTAVQSATFAKLAAENALSSRLPEFCSAANLLCFPDLSPSLAKHDRDSNFAVYSNR 120
           KVSPLTAVQSA FAKLAA+NALSSRLPEFCSAANLLCFPDLSPSLAKHD  SNFAVYSN+
Sbjct: 61  KVSPLTAVQSAAFAKLAAQNALSSRLPEFCSAANLLCFPDLSPSLAKHDSTSNFAVYSNK 120

Query: 121 NFTAYGTQRSGGIDSFKNYSNDDNVIVDSFRRYSHEALGHKDQFTTYATGTNVPDQSFNT 180
           NFT YGTQRSGGIDSFKNYSN DNV+VDSFRRYSHEALGH DQF+TY T TNVPDQSFNT
Sbjct: 121 NFTTYGTQRSGGIDSFKNYSNGDNVVVDSFRRYSHEALGHTDQFSTYGTDTNVPDQSFNT 180

Query: 181 YGSKAVGGDGKFTTYEESVNVPNLRFTSYSSDATGRSHTFKSYSENANAGDQSFANYGKR 240
           Y SKAVGG+G FTTYE+SVNVPNLRFT+YSSDATGRS +FKSYSENANAGDQSF NYGKR
Sbjct: 181 YASKAVGGNGGFTTYEQSVNVPNLRFTTYSSDATGRSQSFKSYSENANAGDQSFVNYGKR 240

Query: 241 GNGGPNEFAGYGTNANVVGSGFSSYGSEGNGANDTFTNYGNEQNNPQNNFRNYGEGGNGA 300
           GNGGPNEF GYGTNANV+GSGFSSYG+ GNGANDTFTNYGNEQNNPQNNFR+YG+GGNGA
Sbjct: 241 GNGGPNEFTGYGTNANVIGSGFSSYGANGNGANDTFTNYGNEQNNPQNNFRSYGDGGNGA 300

Query: 301 IESFSSYRDQANVGDDSFQSYSKNSNSAKVNFANYGKSFNEGTDKFSGYGQNANGQSVGF 360
           IESFSSYRDQANVGDDSFQSYSKNSNSAKVNFANYGKSFNEGTDKFSGYGQN NGQSVGF
Sbjct: 301 IESFSSYRDQANVGDDSFQSYSKNSNSAKVNFANYGKSFNEGTDKFSGYGQNGNGQSVGF 360

Query: 361 KIYGVNTTFKDYAKQGISFAKYSNVSSGGGKMSVSGSLAKRWVEPGKFFRESMLKKGTVM 420
           K YGVNTTFKDYAKQGISFAKYSNVSSGG KMSVS SLAKRWVEPGKFFRESML+KGTVM
Sbjct: 361 KTYGVNTTFKDYAKQGISFAKYSNVSSGGAKMSVSESLAKRWVEPGKFFRESMLRKGTVM 420

Query: 421 AMPDIRDKMPKRSFLPRSIVSKLPFSSSKVGVMKEIFQAGENSSMETMMTETLRECERAP 480
           AMPDIRDKMPKRSFLPRSI+SKLPFSSSK+ VMK+IF AG+NSSMETMMTETLR+CER P
Sbjct: 421 AMPDIRDKMPKRSFLPRSILSKLPFSSSKLEVMKQIFHAGDNSSMETMMTETLRDCERTP 480

Query: 481 SAGETKRCVGSVEDMIDFATSVLGRNVVVRTTENTKGSKGKVMIGAVKGINGGKVTQSVS 540
           SAGETKRCVGSVEDMIDFATSVLGRNVVVRTTENTKGSKG +MIGAVKGI GGKVT+SVS
Sbjct: 481 SAGETKRCVGSVEDMIDFATSVLGRNVVVRTTENTKGSKGNIMIGAVKGIEGGKVTESVS 540

Query: 541 CHQSLFPYLLYYCHSVPKVRVYEADLLDPSSKATINHGVAICHLDTSAWSPAHGAFIALG 600
           CHQSLFPYLLYYCHSVPKVRVYEA+LLDP+SKATINHGVAICHLDTSAWSPAHGAFIALG
Sbjct: 541 CHQSLFPYLLYYCHSVPKVRVYEAELLDPTSKATINHGVAICHLDTSAWSPAHGAFIALG 600

Query: 601 SAPGRVEVCHWIFENDMTWAVAD 624
           SAPGRVEVCHWIFENDMTW+VAD
Sbjct: 601 SAPGRVEVCHWIFENDMTWSVAD 623

BLAST of Lag0005327 vs. ExPASy TrEMBL
Match: A0A0A0LK44 (BURP domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_2G348230 PE=4 SV=1)

HSP 1 Score: 1128.2 bits (2917), Expect = 0.0e+00
Identity = 561/623 (90.05%), Postives = 593/623 (95.18%), Query Frame = 0

Query: 1   MRRLPAPLVVPLSLFIFLLSALNVSAAGDEDNPFTPKAFLNRYWNKEVRNDLPKPSFLFS 60
           M RLP   VVPL LFIF LS+LNVS AGDED+PFTPKAFLNRYWNKEVRND PKPSFLFS
Sbjct: 1   MHRLPTLYVVPLYLFIFSLSSLNVSTAGDEDDPFTPKAFLNRYWNKEVRNDFPKPSFLFS 60

Query: 61  KVSPLTAVQSATFAKLAAENALSSRLPEFCSAANLLCFPDLSPSLAKHDRDSNFAVYSNR 120
           K SPLTAVQSA FAKLAAENALSSRLP+FCSAANLLCFPDLSPSLAKHDRDSNFAVYSN+
Sbjct: 61  KASPLTAVQSAAFAKLAAENALSSRLPDFCSAANLLCFPDLSPSLAKHDRDSNFAVYSNK 120

Query: 121 NFTAYGTQRSGGIDSFKNYSNDDNVIVDSFRRYSHEALGHKDQFTTYATGTNVPDQSFNT 180
           NFT YGTQRSGGIDSFKNYSN DNV+VDSFRRYSHEALGH D+F+ Y T TNVPDQSFNT
Sbjct: 121 NFTTYGTQRSGGIDSFKNYSNGDNVVVDSFRRYSHEALGHTDRFSVYGTDTNVPDQSFNT 180

Query: 181 YGSKAVGGDGKFTTYEESVNVPNLRFTSYSSDATGRSHTFKSYSENANAGDQSFANYGKR 240
           Y SKA+GGDGKFTTYE+SVNVPNLRFT+YSSDATGRS +FKSYSENANAGDQSF NYGKR
Sbjct: 181 YASKAIGGDGKFTTYEQSVNVPNLRFTTYSSDATGRSQSFKSYSENANAGDQSFVNYGKR 240

Query: 241 GNGGPNEFAGYGTNANVVGSGFSSYGSEGNGANDTFTNYGNEQNNPQNNFRNYGEGGNGA 300
           GNGGPNEF GYGT+ANV+GS FSSYG+EGNGANDTFTNYGN+QNNPQNNFR+YGEGGNGA
Sbjct: 241 GNGGPNEFTGYGTSANVIGSRFSSYGAEGNGANDTFTNYGNDQNNPQNNFRSYGEGGNGA 300

Query: 301 IESFSSYRDQANVGDDSFQSYSKNSNSAKVNFANYGKSFNEGTDKFSGYGQNANGQSVGF 360
           IESFSSYRDQANVGDDSFQSYSKNSNSAKVNFA+YGKSFNEGTDKFSGYGQN NGQSVGF
Sbjct: 301 IESFSSYRDQANVGDDSFQSYSKNSNSAKVNFASYGKSFNEGTDKFSGYGQNGNGQSVGF 360

Query: 361 KIYGVNTTFKDYAKQGISFAKYSNVSSGGGKMSVSGSLAKRWVEPGKFFRESMLKKGTVM 420
           K YGVNTTFKDYAKQGI+FAKYSNVSSGG KMSVSGSLAKRWVEPGKFFRESML+KG+VM
Sbjct: 361 KTYGVNTTFKDYAKQGINFAKYSNVSSGGAKMSVSGSLAKRWVEPGKFFRESMLRKGSVM 420

Query: 421 AMPDIRDKMPKRSFLPRSIVSKLPFSSSKVGVMKEIFQAGENSSMETMMTETLRECERAP 480
           AMPDIRDKMPKRSFLPRSI+SKLPFSSSK+GVMK+IF AG+NSSMETMMTET+RECER P
Sbjct: 421 AMPDIRDKMPKRSFLPRSILSKLPFSSSKLGVMKQIFHAGDNSSMETMMTETVRECERLP 480

Query: 481 SAGETKRCVGSVEDMIDFATSVLGRNVVVRTTENTKGSKGKVMIGAVKGINGGKVTQSVS 540
           SAGETKRCVGSVEDMIDFATSVLGRNVVV+TTENTKGSKG +MIG VKG+NGGKVT+SVS
Sbjct: 481 SAGETKRCVGSVEDMIDFATSVLGRNVVVQTTENTKGSKGNIMIGKVKGMNGGKVTESVS 540

Query: 541 CHQSLFPYLLYYCHSVPKVRVYEADLLDPSSKATINHGVAICHLDTSAWSPAHGAFIALG 600
           CHQSLFPYLLYYCHSVPKVRVYE DLLDP++KAT+NHGVAICHLDTSAWSPAHGAFIALG
Sbjct: 541 CHQSLFPYLLYYCHSVPKVRVYEVDLLDPATKATVNHGVAICHLDTSAWSPAHGAFIALG 600

Query: 601 SAPGRVEVCHWIFENDMTWAVAD 624
           SAPGRVEVCHWIFENDMTW VAD
Sbjct: 601 SAPGRVEVCHWIFENDMTWTVAD 623

BLAST of Lag0005327 vs. ExPASy TrEMBL
Match: A0A6J1K271 (polygalacturonase 1 beta-like protein 3 OS=Cucurbita maxima OX=3661 GN=LOC111491752 PE=4 SV=1)

HSP 1 Score: 1126.7 bits (2913), Expect = 0.0e+00
Identity = 565/623 (90.69%), Postives = 592/623 (95.02%), Query Frame = 0

Query: 1   MRRLPAPLVVPLSLFIFLLSALNVSAAGDEDNPFTPKAFLNRYWNKEVRNDLPKPSFLFS 60
           M  LPAPL+V L LFIFLLS LNV AA DEDNPFTPKAFLNRYWNKEVRND+PKP+FLFS
Sbjct: 1   MHTLPAPLLVLLYLFIFLLSPLNVPAAADEDNPFTPKAFLNRYWNKEVRNDMPKPTFLFS 60

Query: 61  KVSPLTAVQSATFAKLAAENALSSRLPEFCSAANLLCFPDLSPSLAKHDRDSNFAVYSNR 120
           KVSPLTAVQSA FAKLAA+NALSSRLPEFCSAANLLCFPDLSPSLAKHD  SNFAVYSN+
Sbjct: 61  KVSPLTAVQSAAFAKLAAQNALSSRLPEFCSAANLLCFPDLSPSLAKHDSTSNFAVYSNK 120

Query: 121 NFTAYGTQRSGGIDSFKNYSNDDNVIVDSFRRYSHEALGHKDQFTTYATGTNVPDQSFNT 180
           NFT YGTQRSGGIDSFKNYSN DNV+VDSFRRYSHEALGH DQF+TY T TNVPDQSFNT
Sbjct: 121 NFTTYGTQRSGGIDSFKNYSNGDNVVVDSFRRYSHEALGHTDQFSTYGTDTNVPDQSFNT 180

Query: 181 YGSKAVGGDGKFTTYEESVNVPNLRFTSYSSDATGRSHTFKSYSENANAGDQSFANYGKR 240
           Y SKAVGG+G FTTYE+SVNVPNLRFT+YSSDATGRS +FKSYSENANAGDQSF NYGKR
Sbjct: 181 YASKAVGGNGGFTTYEQSVNVPNLRFTTYSSDATGRSQSFKSYSENANAGDQSFVNYGKR 240

Query: 241 GNGGPNEFAGYGTNANVVGSGFSSYGSEGNGANDTFTNYGNEQNNPQNNFRNYGEGGNGA 300
           GNGGPNEF GYGTNANV+GSGFSSYG+ GNGANDTFTNYGNEQNNPQNNFR+YG+GGNGA
Sbjct: 241 GNGGPNEFTGYGTNANVIGSGFSSYGANGNGANDTFTNYGNEQNNPQNNFRSYGDGGNGA 300

Query: 301 IESFSSYRDQANVGDDSFQSYSKNSNSAKVNFANYGKSFNEGTDKFSGYGQNANGQSVGF 360
           IESFSSYRDQANVGDDSFQSYSKNSNSAKVNFANYGKSFNEGTDKFSGYGQN NGQSVGF
Sbjct: 301 IESFSSYRDQANVGDDSFQSYSKNSNSAKVNFANYGKSFNEGTDKFSGYGQNGNGQSVGF 360

Query: 361 KIYGVNTTFKDYAKQGISFAKYSNVSSGGGKMSVSGSLAKRWVEPGKFFRESMLKKGTVM 420
           K YGVNTTFKDYAKQGISFAKYSNVSSGG KMSVS SLAKRWVEPGKFFRESML+KGTV+
Sbjct: 361 KTYGVNTTFKDYAKQGISFAKYSNVSSGGAKMSVSESLAKRWVEPGKFFRESMLRKGTVV 420

Query: 421 AMPDIRDKMPKRSFLPRSIVSKLPFSSSKVGVMKEIFQAGENSSMETMMTETLRECERAP 480
           AMPDIRDKMPKRSFLPRSI+SKLPFSSSK+ VMK+IF AG+NSSMETMMTETLR+CER P
Sbjct: 421 AMPDIRDKMPKRSFLPRSILSKLPFSSSKLEVMKQIFHAGDNSSMETMMTETLRDCERTP 480

Query: 481 SAGETKRCVGSVEDMIDFATSVLGRNVVVRTTENTKGSKGKVMIGAVKGINGGKVTQSVS 540
           SAGETKRCVGSVEDMIDFATSVLGRNVVVRTTENTKGSKG +MIGAVKGI GGKVT+SVS
Sbjct: 481 SAGETKRCVGSVEDMIDFATSVLGRNVVVRTTENTKGSKGNIMIGAVKGIEGGKVTESVS 540

Query: 541 CHQSLFPYLLYYCHSVPKVRVYEADLLDPSSKATINHGVAICHLDTSAWSPAHGAFIALG 600
           CHQSLFPYLLYYCHSVPKVRVYEA+LLDP+SKA INHGVAICHLDTSAWSPAHGAFIALG
Sbjct: 541 CHQSLFPYLLYYCHSVPKVRVYEAELLDPTSKAIINHGVAICHLDTSAWSPAHGAFIALG 600

Query: 601 SAPGRVEVCHWIFENDMTWAVAD 624
           SAPGRVEVCHWIFENDMTW+VAD
Sbjct: 601 SAPGRVEVCHWIFENDMTWSVAD 623

BLAST of Lag0005327 vs. TAIR 10
Match: AT1G70370.1 (polygalacturonase 2 )

HSP 1 Score: 800.4 bits (2066), Expect = 1.0e-231
Identity = 391/595 (65.71%), Postives = 472/595 (79.33%), Query Frame = 0

Query: 30  EDNPFTPKAFLNRYWNKEVRNDLPKPSFLFSKVSPLTAVQSATFAKLAAENALSSRLPEF 89
           E+NPFTPKA L RYWNKE+R   P+  FL SK SPL AV SATF+KLAA N+L +R P+F
Sbjct: 33  EENPFTPKASLVRYWNKEIRGQSPRSEFLISKASPLNAVDSATFSKLAAANSLPTRFPDF 92

Query: 90  CSAANLLCFPDLSPSLAKHDRDSNFAVYSNRNFTAYGTQRSGGIDSFKNYSNDDNVIVDS 149
           CSAANL CFPDL  SL KHD D  F+VY  +NFT YG  R+GG DSFKNYS D NV+ DS
Sbjct: 93  CSAANLFCFPDLGASLEKHDDDVKFSVYDQKNFTNYGNARAGGADSFKNYSKDGNVVTDS 152

Query: 150 FRRYSHEALGHKDQFTTYATGTNVPDQSFNTYGSKAVGGDGKFTTYEESVNVPNLRFTSY 209
           FRRYS  A GH D+FT Y   +NV ++ FN+YG+   GG G FT Y+ +VN P  RFT+Y
Sbjct: 153 FRRYSRNAAGHDDKFTVYGENSNVVEEGFNSYGTFGTGGAGDFTNYQNNVNNPTSRFTAY 212

Query: 210 SSDATGRSHTFKSYSENANAGD-QSFANYGKRGNGGPNEFAGYGTNANVVGSGFSSYGSE 269
           S    GRS TFK+Y+  ANAG+ QSF +YGK GNG PNEF  YG ++NV+GSGFS+YG  
Sbjct: 213 SDGGNGRSQTFKTYTHEANAGNGQSFTSYGKNGNGVPNEFTSYGVSSNVIGSGFSNYGES 272

Query: 270 GNGANDTFTNYGNEQNNPQNNFRNYGEGGNGAIESFSSYRDQANVGDDSFQSYSKNSNSA 329
           GN ANDTFT+YG++ N PQNNF NYG  GN A+++F++YRD+ANVGDDSF SY+K+SNS 
Sbjct: 273 GNAANDTFTSYGSDGNVPQNNFNNYGASGNAAVDTFANYRDKANVGDDSFSSYAKDSNSE 332

Query: 330 KVNFANYGKSFNEGTDKFSGYGQNANGQSVGFKIYGVNTTFKDYAKQGISFAKYSNVSSG 389
           KVNF NYG+SFN G++ F+GYG+ A G  + FK Y  N+TFKDYAK+G++FAKY NVS+ 
Sbjct: 333 KVNFVNYGQSFNPGSETFTGYGKGAEGSKLSFKTYTPNSTFKDYAKKGVAFAKY-NVSTT 392

Query: 390 GGKMSVSGSLAKRWVEPGKFFRESMLKKGTVMAMPDIRDKMPKRSFLPRSIVSKLPFSSS 449
                  G    +W+EPGKFFRES LK+GTV+ MPDI+DKMPKRSFLPRSI++KLPFS+S
Sbjct: 393 TANTVGDGKTVNKWIEPGKFFRESSLKEGTVIPMPDIKDKMPKRSFLPRSIITKLPFSTS 452

Query: 450 KVGVMKEIFQAGENSSMETMMTETLRECERAPSAGETKRCVGSVEDMIDFATSVLGRNVV 509
           K+G +K IF A ENS+M  ++T+ + ECER PS GETKRCVGS EDMIDFATSVLGR+VV
Sbjct: 453 KLGEIKRIFHAVENSTMGGIITDAVTECERPPSVGETKRCVGSAEDMIDFATSVLGRSVV 512

Query: 510 VRTTENTKGSKGKVMIGAVKGINGGKVTQSVSCHQSLFPYLLYYCHSVPKVRVYEADLLD 569
           +RTTEN  GSK KV+IG V GINGGK+T++VSCHQSL+PYLLYYCHSVPKVRVYEADLL+
Sbjct: 513 LRTTENVAGSKEKVVIGKVNGINGGKLTKAVSCHQSLYPYLLYYCHSVPKVRVYEADLLE 572

Query: 570 PSSKATINHGVAICHLDTSAWSPAHGAFIALGSAPGRVEVCHWIFENDMTWAVAD 624
            +SK  INHG+AICH+DTS+W P+HGAF+ALGS PGR+EVCHWIFENDM WA+AD
Sbjct: 573 LNSKKKINHGIAICHMDTSSWGPSHGAFLALGSKPGRIEVCHWIFENDMNWAIAD 626

BLAST of Lag0005327 vs. TAIR 10
Match: AT1G70370.2 (polygalacturonase 2 )

HSP 1 Score: 800.4 bits (2066), Expect = 1.0e-231
Identity = 391/595 (65.71%), Postives = 472/595 (79.33%), Query Frame = 0

Query: 30  EDNPFTPKAFLNRYWNKEVRNDLPKPSFLFSKVSPLTAVQSATFAKLAAENALSSRLPEF 89
           E+NPFTPKA L RYWNKE+R   P+  FL SK SPL AV SATF+KLAA N+L +R P+F
Sbjct: 33  EENPFTPKASLVRYWNKEIRGQSPRSEFLISKASPLNAVDSATFSKLAAANSLPTRFPDF 92

Query: 90  CSAANLLCFPDLSPSLAKHDRDSNFAVYSNRNFTAYGTQRSGGIDSFKNYSNDDNVIVDS 149
           CSAANL CFPDL  SL KHD D  F+VY  +NFT YG  R+GG DSFKNYS D NV+ DS
Sbjct: 93  CSAANLFCFPDLGASLEKHDDDVKFSVYDQKNFTNYGNARAGGADSFKNYSKDGNVVTDS 152

Query: 150 FRRYSHEALGHKDQFTTYATGTNVPDQSFNTYGSKAVGGDGKFTTYEESVNVPNLRFTSY 209
           FRRYS  A GH D+FT Y   +NV ++ FN+YG+   GG G FT Y+ +VN P  RFT+Y
Sbjct: 153 FRRYSRNAAGHDDKFTVYGENSNVVEEGFNSYGTFGTGGAGDFTNYQNNVNNPTSRFTAY 212

Query: 210 SSDATGRSHTFKSYSENANAGD-QSFANYGKRGNGGPNEFAGYGTNANVVGSGFSSYGSE 269
           S    GRS TFK+Y+  ANAG+ QSF +YGK GNG PNEF  YG ++NV+GSGFS+YG  
Sbjct: 213 SDGGNGRSQTFKTYTHEANAGNGQSFTSYGKNGNGVPNEFTSYGVSSNVIGSGFSNYGES 272

Query: 270 GNGANDTFTNYGNEQNNPQNNFRNYGEGGNGAIESFSSYRDQANVGDDSFQSYSKNSNSA 329
           GN ANDTFT+YG++ N PQNNF NYG  GN A+++F++YRD+ANVGDDSF SY+K+SNS 
Sbjct: 273 GNAANDTFTSYGSDGNVPQNNFNNYGASGNAAVDTFANYRDKANVGDDSFSSYAKDSNSE 332

Query: 330 KVNFANYGKSFNEGTDKFSGYGQNANGQSVGFKIYGVNTTFKDYAKQGISFAKYSNVSSG 389
           KVNF NYG+SFN G++ F+GYG+ A G  + FK Y  N+TFKDYAK+G++FAKY NVS+ 
Sbjct: 333 KVNFVNYGQSFNPGSETFTGYGKGAEGSKLSFKTYTPNSTFKDYAKKGVAFAKY-NVSTT 392

Query: 390 GGKMSVSGSLAKRWVEPGKFFRESMLKKGTVMAMPDIRDKMPKRSFLPRSIVSKLPFSSS 449
                  G    +W+EPGKFFRES LK+GTV+ MPDI+DKMPKRSFLPRSI++KLPFS+S
Sbjct: 393 TANTVGDGKTVNKWIEPGKFFRESSLKEGTVIPMPDIKDKMPKRSFLPRSIITKLPFSTS 452

Query: 450 KVGVMKEIFQAGENSSMETMMTETLRECERAPSAGETKRCVGSVEDMIDFATSVLGRNVV 509
           K+G +K IF A ENS+M  ++T+ + ECER PS GETKRCVGS EDMIDFATSVLGR+VV
Sbjct: 453 KLGEIKRIFHAVENSTMGGIITDAVTECERPPSVGETKRCVGSAEDMIDFATSVLGRSVV 512

Query: 510 VRTTENTKGSKGKVMIGAVKGINGGKVTQSVSCHQSLFPYLLYYCHSVPKVRVYEADLLD 569
           +RTTEN  GSK KV+IG V GINGGK+T++VSCHQSL+PYLLYYCHSVPKVRVYEADLL+
Sbjct: 513 LRTTENVAGSKEKVVIGKVNGINGGKLTKAVSCHQSLYPYLLYYCHSVPKVRVYEADLLE 572

Query: 570 PSSKATINHGVAICHLDTSAWSPAHGAFIALGSAPGRVEVCHWIFENDMTWAVAD 624
            +SK  INHG+AICH+DTS+W P+HGAF+ALGS PGR+EVCHWIFENDM WA+AD
Sbjct: 573 LNSKKKINHGIAICHMDTSSWGPSHGAFLALGSKPGRIEVCHWIFENDMNWAIAD 626

BLAST of Lag0005327 vs. TAIR 10
Match: AT1G23760.1 (BURP domain-containing protein )

HSP 1 Score: 789.6 bits (2038), Expect = 1.8e-228
Identity = 394/624 (63.14%), Postives = 473/624 (75.80%), Query Frame = 0

Query: 15  FIFLLSALN-----VSAAGD---------EDNPFTPKAFLNRYWNKEVRNDLPKPSFLFS 74
           F+FLL  L+     V A G+         E NPFTPKA L RYWNK++ +  P  +FL S
Sbjct: 5   FVFLLPFLSRLYHVVIAGGERLTNGGFSPEINPFTPKASLVRYWNKQIHHQSPGSAFLLS 64

Query: 75  KVSPLTAVQSATFAKLAAENALSSRLPEFCSAANLLCFPDLSPSLAKHDRDSNFAVYSNR 134
           K SPLTAV SATFAKLA+ENAL  RLP+FCSAANL CFPDL P+L KH  D  F+VY  R
Sbjct: 65  KASPLTAVASATFAKLASENALPDRLPDFCSAANLFCFPDLGPTLEKHSNDVKFSVYDQR 124

Query: 135 NFTAYGTQRSGGIDSFKNYSNDDNVIVDSFRRYSHEALGHKDQFTTYATGTNVPDQSFNT 194
           NFT YGT   GG DSFK YS + N + DSFRRYS +A GH+D+FT YA  +NV ++ FN+
Sbjct: 125 NFTNYGTIHPGGADSFKKYSQNGNAVTDSFRRYSRDAAGHEDKFTGYADNSNVVEEKFNS 184

Query: 195 YGSKAVGGDGKFTTYEESVNVPNLRFTSYSSDATGRSHTFKSYSENANAG-DQSFANYGK 254
           YG+ A GG G F  Y+  VN P  RF +YS +  GR+ TFK+Y+  AN+G  QSF +YGK
Sbjct: 185 YGTSATGGSGDFKNYQSGVNNPTTRFKAYSDEGNGRAQTFKTYTHEANSGPGQSFTSYGK 244

Query: 255 RGNGGPNEFAGYGTNANVVGSGFSSYGSEGNGANDTFTNYGNEQNNPQNNFRNYGEGGNG 314
            GNG PNEF+ YG ++NV+GS FS+YG  GN ANDTF  YG + N PQNNF++YG  GN 
Sbjct: 245 NGNGAPNEFSSYGVSSNVIGSDFSNYGENGNAANDTFKGYGGDGNVPQNNFKSYGASGNA 304

Query: 315 AIESFSSYRDQANVGDDSFQSYSKNSNSAKVNFANYGKSFNEGTDKFSGYGQNANGQSVG 374
           A+++F +YRD ANVGDDSF SY+KNSN  KV+F NYGKS N G++ FSGYG+ A G  + 
Sbjct: 305 AVDTFLNYRDNANVGDDSFSSYAKNSNFEKVDFVNYGKSINPGSESFSGYGKGAEGNKID 364

Query: 375 FKIYGVNTTFKDYAKQGISFAKYSNVSSGGGKMSVSGSLAKRWVEPGKFFRESMLKKGTV 434
           FK Y  N+TFKDY K G+ FAKY+  S GGGK         +WVEPGKFFRESMLK+GT+
Sbjct: 365 FKTYTQNSTFKDYTKTGVEFAKYNRSSLGGGK------TVNKWVEPGKFFRESMLKEGTL 424

Query: 435 MAMPDIRDKMPKRSFLPRSIVSKLPFSSSKVGVMKEIFQAGENSSMETMMTETLRECERA 494
           + MPDI+DKMPKRSFLPRSIVSKLPFS+SK+  +K +F A +NS+ME ++T+ +RECER 
Sbjct: 425 IWMPDIKDKMPKRSFLPRSIVSKLPFSTSKIAEIKRVFHANDNSTMEGIITDAVRECERP 484

Query: 495 PSAGETKRCVGSVEDMIDFATSVLGRNVVVRTTENTKGSKGKVMIGAVKGINGGKVTQSV 554
           P+  ETKRCVGS EDMIDFATSVLGR+VV+RTTE+  GSK KVMIG V GINGG+VT+SV
Sbjct: 485 PTVSETKRCVGSAEDMIDFATSVLGRSVVLRTTESVAGSKEKVMIGKVNGINGGRVTKSV 544

Query: 555 SCHQSLFPYLLYYCHSVPKVRVYEADLLDPSSKATINHGVAICHLDTSAWSPAHGAFIAL 614
           SCHQSL+PYLLYYCHSVPKVRVYE+DLLDP SKA INHG+AICH+DTSAW   HGAF+ L
Sbjct: 545 SCHQSLYPYLLYYCHSVPKVRVYESDLLDPKSKAKINHGIAICHMDTSAWGANHGAFMLL 604

Query: 615 GSAPGRVEVCHWIFENDMTWAVAD 624
           GS PG++EVCHWIFENDM WA+AD
Sbjct: 605 GSRPGQIEVCHWIFENDMNWAIAD 622

BLAST of Lag0005327 vs. TAIR 10
Match: AT1G60390.1 (polygalacturonase 1 )

HSP 1 Score: 759.2 bits (1959), Expect = 2.6e-219
Identity = 385/619 (62.20%), Postives = 452/619 (73.02%), Query Frame = 0

Query: 15  FIFLLSALNVSAAG---------DEDNPFTPKAFLNRYWNKEVRNDLPKPSFLFSKVSPL 74
           F  + S+ NV  AG           +NPFTPKA L RYWN  +  D PKPSF  SK SPL
Sbjct: 12  FFCIFSSSNVHFAGAKQTAGNITPSENPFTPKASLIRYWNNHINGDSPKPSFFLSKASPL 71

Query: 75  TAVQSATFAKLAAENALSSRLPEFCSAANLLCFPDLSP-SLAKHDRDSNFAVYSNRNFTA 134
           TAV S  FA LA+ +AL++   +FCSAA L CFP+L+  SL KH  D NFA YS +NFT 
Sbjct: 72  TAVDSTRFASLASNHALNTHHSDFCSAAKLFCFPELAAHSLEKHGDDVNFAAYSGKNFTN 131

Query: 135 YGTQRSGGIDSFKNYSNDDNVIVDSFRRYSHEALGHKDQFTTYATGTNVPDQSFNTYGSK 194
           YG+ R  G DSFKNYS  DN+ VDSFRRYS  + GH D FT YA   NV DQSF TY + 
Sbjct: 132 YGSDRLSGADSFKNYSGGDNIAVDSFRRYSRNSAGHDDGFTNYAGEVNVADQSFTTYATG 191

Query: 195 AVGGDGKFTTYEESVNVPNLRFTSYSSDATGRSHTFKSYSENANAGDQSFANYGKRGNGG 254
             GG G+FT Y    N PN RFTSYS  A GRS TF +YSEN N G QSF +Y K GNG 
Sbjct: 192 TTGGSGEFTNYNTDANEPNGRFTSYSDKANGRSQTFTTYSENGNTGYQSFTSYSKNGNGA 251

Query: 255 PNEFAGYGTNANVVGSGFSSYGSEGNGANDTFTNYGNEQNNPQNNFRNYGEGGNGAIESF 314
           PNEF+GYGT +NVV +GF+ YG   NGAND+FT+YG   N P N F+ YG+GGNGA+  F
Sbjct: 252 PNEFSGYGTGSNVVNTGFTKYGESANGANDSFTSYGENGNVPVNEFKGYGDGGNGAVYGF 311

Query: 315 SSYRDQANVGDDSFQSYSKNSNSAKVNFANYGKSFNEGTDKFSGYGQNANGQSVGFKIYG 374
            +YRDQ+N+G DSF SY+KNSN+ KVNF NYGKSFN G+D F+GYGQ+  G +V FK YG
Sbjct: 312 KNYRDQSNIGVDSFSSYAKNSNNEKVNFVNYGKSFNLGSDNFTGYGQDNVGGNVSFKTYG 371

Query: 375 VNTTFKDYAKQGISFAKYSNVSSGGGKMSVSGSLAKRWVEPGKFFRESMLKKGTVMAMPD 434
              +FK Y K G+ FA+YSN       +S +G    +WVE GKFFRE+MLK+GT+M MPD
Sbjct: 372 QGQSFKVYTKDGVVFARYSN------NVSSNGKTVNKWVEEGKFFREAMLKEGTLMQMPD 431

Query: 435 IRDKMPKRSFLPRSIVSKLPFSSSKVGVMKEIFQAGENSSMETMMTETLRECERAPSAGE 494
           I+DKMPKR+FLPR+IV  LPFSSS +G +  +F AGENSSM  +++  + ECER  S GE
Sbjct: 432 IKDKMPKRTFLPRNIVKNLPFSSSTIGEIWRVFGAGENSSMAGIISSAVSECERPASHGE 491

Query: 495 TKRCVGSVEDMIDFATSVLGRNVVVRTTENTKGSKGKVMIGAVKGINGGKVTQSVSCHQS 554
           TKRCVGS EDMIDFATSVLGR VVVRTTEN  GSK KV+IG V GINGG VT++VSCHQS
Sbjct: 492 TKRCVGSAEDMIDFATSVLGRGVVVRTTENVVGSKKKVVIGKVNGINGGDVTRAVSCHQS 551

Query: 555 LFPYLLYYCHSVPKVRVYEADLLDPSSKATINHGVAICHLDTSAWSPAHGAFIALGSAPG 614
           L+PYLLYYCHSVP+VRVYE DLLDP S   INHGVAICH+DTSAWSP+HGAF+ALGS PG
Sbjct: 552 LYPYLLYYCHSVPRVRVYETDLLDPKSLEKINHGVAICHIDTSAWSPSHGAFLALGSGPG 611

Query: 615 RVEVCHWIFENDMTWAVAD 624
           ++EVCHWIFENDMTW + D
Sbjct: 612 QIEVCHWIFENDMTWNIID 624

BLAST of Lag0005327 vs. TAIR 10
Match: AT5G25610.1 (BURP domain-containing protein )

HSP 1 Score: 117.9 bits (294), Expect = 3.0e-26
Identity = 70/194 (36.08%), Postives = 97/194 (50.00%), Query Frame = 0

Query: 431 KRSFLPRSIVSKLPFSSSKVGVMKEIFQAGENSSMETMMTETLRECERAPSAGETKRCVG 490
           K +FLPR     +PF S K     + F     S    MM +T+ ECE    +GE K C  
Sbjct: 201 KTAFLPRGEAETVPFGSEKFSETLKRFSVEAGSEEAEMMKKTIEECEARKVSGEEKYCAT 260

Query: 491 SVEDMIDFATSVLGR-NVVVRTTE----NTKGSKGKVMIGAVKGINGGKVTQSVSCHQSL 550
           S+E M+DF+ S LG+ +V   +TE    N    K K+    VK ++  K   SV CH+  
Sbjct: 261 SLESMVDFSVSKLGKYHVRAVSTEVAKKNAPMQKYKIAAAGVKKLSDDK---SVVCHKQK 320

Query: 551 FPYLLYYCHSVPKVRVYEADLLDPSSKATINHGVAICHLDTSAWSPAHGAFIALGSAPGR 610
           +P+ ++YCH      VY   L   +        VA+CH +TSAW+P H AF  L   PG 
Sbjct: 321 YPFAVFYCHKAMMTTVYAVPLEGENGMRA--KAVAVCHKNTSAWNPNHLAFKVLKVKPGT 380

Query: 611 VEVCHWIFENDMTW 620
           V VCH++ E  + W
Sbjct: 381 VPVCHFLPETHVVW 389

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
XP_038884819.10.0e+0092.30polygalacturonase 1 beta-like protein 3 [Benincasa hispida][more]
XP_008444317.10.0e+0090.69PREDICTED: polygalacturonase 1 beta-like protein 3 [Cucumis melo] >KAA0061089.1 ... [more]
XP_023546090.10.0e+0091.33polygalacturonase 1 beta-like protein 3 [Cucurbita pepo subsp. pepo][more]
XP_022961767.10.0e+0091.17polygalacturonase 1 beta-like protein 3 [Cucurbita moschata][more]
XP_004142981.10.0e+0090.05polygalacturonase 1 beta-like protein 3 [Cucumis sativus] >KGN62285.1 hypothetic... [more]
Match NameE-valueIdentityDescription
P929901.4e-23065.71Polygalacturonase 1 beta-like protein 3 OS=Arabidopsis thaliana OX=3702 GN=PGL3 ... [more]
P929822.5e-22763.14Polygalacturonase 1 beta-like protein 1 OS=Arabidopsis thaliana OX=3702 GN=PGL1 ... [more]
O807603.6e-21862.20Polygalacturonase 1 beta-like protein 2 OS=Arabidopsis thaliana OX=3702 GN=PGL2 ... [more]
Q401611.8e-21761.77Polygalacturonase-1 non-catalytic subunit beta OS=Solanum lycopersicum OX=4081 G... [more]
P932188.6e-21260.45Polygalacturonase non-catalytic subunit AroGP3 OS=Solanum lycopersicum OX=4081 G... [more]
Match NameE-valueIdentityDescription
A0A5A7V5U80.0e+0090.69Polygalacturonase 1 beta-like protein 3 OS=Cucumis melo var. makuwa OX=1194695 G... [more]
A0A1S3B9L40.0e+0090.69polygalacturonase 1 beta-like protein 3 OS=Cucumis melo OX=3656 GN=LOC103487686 ... [more]
A0A6J1HB030.0e+0091.17polygalacturonase 1 beta-like protein 3 OS=Cucurbita moschata OX=3662 GN=LOC1114... [more]
A0A0A0LK440.0e+0090.05BURP domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_2G348230 PE=4 S... [more]
A0A6J1K2710.0e+0090.69polygalacturonase 1 beta-like protein 3 OS=Cucurbita maxima OX=3661 GN=LOC111491... [more]
Match NameE-valueIdentityDescription
AT1G70370.11.0e-23165.71polygalacturonase 2 [more]
AT1G70370.21.0e-23165.71polygalacturonase 2 [more]
AT1G23760.11.8e-22863.14BURP domain-containing protein [more]
AT1G60390.12.6e-21962.20polygalacturonase 1 [more]
AT5G25610.13.0e-2636.08BURP domain-containing protein [more]
InterPro
Analysis Name: InterPro Annotations of Sponge gourd (AG-4) v1
Date Performed: 2022-08-01
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR004873BURP domainSMARTSM01045BURP_2coord: 406..622
e-value: 2.2E-134
score: 462.4
IPR004873BURP domainPFAMPF03181BURPcoord: 408..620
e-value: 1.2E-65
score: 221.2
IPR004873BURP domainPROSITEPS51277BURPcoord: 408..622
score: 68.435623
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 273..295
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 273..294
NoneNo IPR availablePANTHERPTHR31458:SF2POLYGALACTURONASE 1 BETA-LIKE PROTEIN 2coord: 13..623
NoneNo IPR availablePANTHERPTHR31458POLYGALACTURONASE 1 BETA-LIKE PROTEIN 2coord: 13..623

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Lag0005327.1Lag0005327.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0008152 metabolic process
molecular_function GO:0004650 polygalacturonase activity