Lag0005319 (gene) Sponge gourd (AG‐4) v1

Overview
NameLag0005319
Typegene
OrganismLuffa acutangula (Sponge gourd (AG‐4) v1)
DescriptionPoly [ADP-ribose] polymerase
Locationchr6: 14262804 .. 14264099 (+)
RNA-Seq ExpressionLag0005319
SyntenyLag0005319
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonCDSpolypeptide
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGGAGCACTTCAATATTGAAGAGCGAATCTCCATAACCATCGATGATTACGAGAGCTTAGGCTCTGGGAATTACGAGGGCGATGAGTACGATTCTTTGACTTCGAATCGGTTTAGGTTCCTCCAAGCGAGCGGAATGACGAGGCTCGAAGACAAGGGGGTGGAGCACCAAACTGTAGCCAAGAGCTTCGTTTCAGGTCTAGGGTTGCTTGGGCGGGATGCACGAGTCGTGGCGGTTCATAAGAATTGGAGTTCGGATTCGGGGAAGACGGCGAGGGCGGAAGCGTTTCGGATATTCTCAGACGCTGTGGCCGCCAAATGCGGCGGCGATGCTAATATCAAGTTTGCTTGGTACGGTGCTCCCATCGATGAGATCTGCGACATCGTGTCTCACGGCTTCAGTCATTTTAGCCATAACAATAACGATGCGGACTCTGAATCATCGCATGGCGTCGGCGTCCATTTGTCTCCAGGTAGATTTTCCATCGATTGGTAAGTATTTTCCTCGCCATAGCTTTAATTATCTGCCCTGCCGGCTAATCGACCCTACTAGTACAACGAACATACACGTCTCGAGAATTTCGTTAGAACAGAGATAACAGAGTTGGCTCATGTGGTTTGAATTGTTTTCAATTGCAGCGTCTCGTCTTCGGCAGCGGACCGAAATGGTTTACGACACATGTTATTATGCAGAGTAATATTGGGGAAAGTGGAAGCTATTCCCGTCGGGTCTACGCAATCTCATCCAACTTCAAGCGCATTCGATACGGCCGTAGATAGCCTCTTCGCACCCAGAAAATACATCGTTTGGGCTGCCTTCATGAATTCTCACATACAACCCGCTTACGTCGTAAGCTTCAAGGCCCCTGATCCAAACGGTATGTTTGATTTGTTCTTCTTTTTTTACAATTTAGTGTTTGTATTGGATTCAATTTTTAAATATATATTGTTTCAGATGCCCAAATGAATTCGGTAAGTGTGATGAAGCCAACGTCGCCATGGATGAGTTTTCCTACTCTGATGTCTGCACTTTCGAGGTTCTTGGATCCTGGGAGGATGAGTTTGATACAAAAATGCCACAAGGATTTTGTTGTAAGTGATGCAATATTATTTAAAAATTTTAAAATTTGATTTGCTAATGAGCAAGTTCATGAAATAAAAGATGTAAATTGGGATGCAGAGAAACAAGATTCCGCGGCCACAGCTGATAAACAGAGTGAGGCACATCGCTGGAGACGAGCTGTTGATTGCTCTAATTAAAAAGCATAGAAGAAACGAGGTCATGCCACTTGCCTAG

mRNA sequence

ATGGAGCACTTCAATATTGAAGAGCGAATCTCCATAACCATCGATGATTACGAGAGCTTAGGCTCTGGGAATTACGAGGGCGATGAGTACGATTCTTTGACTTCGAATCGGTTTAGGTTCCTCCAAGCGAGCGGAATGACGAGGCTCGAAGACAAGGGGGTGGAGCACCAAACTGTAGCCAAGAGCTTCGTTTCAGGTCTAGGGTTGCTTGGGCGGGATGCACGAGTCGTGGCGGTTCATAAGAATTGGAGTTCGGATTCGGGGAAGACGGCGAGGGCGGAAGCGTTTCGGATATTCTCAGACGCTGTGGCCGCCAAATGCGGCGGCGATGCTAATATCAAGTTTGCTTGGTACGGTGCTCCCATCGATGAGATCTGCGACATCGTGTCTCACGGCTTCAGTCATTTTAGCCATAACAATAACGATGCGGACTCTGAATCATCGCATGGCGTCGGCGTCCATTTGTCTCCAGGTAGATTTTCCATCGATTGCGTCTCGTCTTCGGCAGCGGACCGAAATGGTTTACGACACATGTTATTATGCAGAGTAATATTGGGGAAAGTGGAAGCTATTCCCGTCGGGTCTACGCAATCTCATCCAACTTCAAGCGCATTCGATACGGCCGTAGATAGCCTCTTCGCACCCAGAAAATACATCGTTTGGGCTGCCTTCATGAATTCTCACATACAACCCGCTTACGTCGTAAGCTTCAAGGCCCCTGATCCAAACGATGCCCAAATGAATTCGGTAAGTGTGATGAAGCCAACGTCGCCATGGATGAGTTTTCCTACTCTGATGTCTGCACTTTCGAGGTTCTTGGATCCTGGGAGGATGAGTTTGATACAAAAATGCCACAAGGATTTTGTTAGAAACAAGATTCCGCGGCCACAGCTGATAAACAGAGTGAGGCACATCGCTGGAGACGAGCTGTTGATTGCTCTAATTAAAAAGCATAGAAGAAACGAGGTCATGCCACTTGCCTAG

Coding sequence (CDS)

ATGGAGCACTTCAATATTGAAGAGCGAATCTCCATAACCATCGATGATTACGAGAGCTTAGGCTCTGGGAATTACGAGGGCGATGAGTACGATTCTTTGACTTCGAATCGGTTTAGGTTCCTCCAAGCGAGCGGAATGACGAGGCTCGAAGACAAGGGGGTGGAGCACCAAACTGTAGCCAAGAGCTTCGTTTCAGGTCTAGGGTTGCTTGGGCGGGATGCACGAGTCGTGGCGGTTCATAAGAATTGGAGTTCGGATTCGGGGAAGACGGCGAGGGCGGAAGCGTTTCGGATATTCTCAGACGCTGTGGCCGCCAAATGCGGCGGCGATGCTAATATCAAGTTTGCTTGGTACGGTGCTCCCATCGATGAGATCTGCGACATCGTGTCTCACGGCTTCAGTCATTTTAGCCATAACAATAACGATGCGGACTCTGAATCATCGCATGGCGTCGGCGTCCATTTGTCTCCAGGTAGATTTTCCATCGATTGCGTCTCGTCTTCGGCAGCGGACCGAAATGGTTTACGACACATGTTATTATGCAGAGTAATATTGGGGAAAGTGGAAGCTATTCCCGTCGGGTCTACGCAATCTCATCCAACTTCAAGCGCATTCGATACGGCCGTAGATAGCCTCTTCGCACCCAGAAAATACATCGTTTGGGCTGCCTTCATGAATTCTCACATACAACCCGCTTACGTCGTAAGCTTCAAGGCCCCTGATCCAAACGATGCCCAAATGAATTCGGTAAGTGTGATGAAGCCAACGTCGCCATGGATGAGTTTTCCTACTCTGATGTCTGCACTTTCGAGGTTCTTGGATCCTGGGAGGATGAGTTTGATACAAAAATGCCACAAGGATTTTGTTAGAAACAAGATTCCGCGGCCACAGCTGATAAACAGAGTGAGGCACATCGCTGGAGACGAGCTGTTGATTGCTCTAATTAAAAAGCATAGAAGAAACGAGGTCATGCCACTTGCCTAG

Protein sequence

MEHFNIEERISITIDDYESLGSGNYEGDEYDSLTSNRFRFLQASGMTRLEDKGVEHQTVAKSFVSGLGLLGRDARVVAVHKNWSSDSGKTARAEAFRIFSDAVAAKCGGDANIKFAWYGAPIDEICDIVSHGFSHFSHNNNDADSESSHGVGVHLSPGRFSIDCVSSSAADRNGLRHMLLCRVILGKVEAIPVGSTQSHPTSSAFDTAVDSLFAPRKYIVWAAFMNSHIQPAYVVSFKAPDPNDAQMNSVSVMKPTSPWMSFPTLMSALSRFLDPGRMSLIQKCHKDFVRNKIPRPQLINRVRHIAGDELLIALIKKHRRNEVMPLA
Homology
BLAST of Lag0005319 vs. NCBI nr
Match: XP_022951201.1 (probable inactive poly [ADP-ribose] polymerase SRO2 [Cucurbita moschata])

HSP 1 Score: 493.0 bits (1268), Expect = 1.9e-135
Identity = 250/329 (75.99%), Postives = 276/329 (83.89%), Query Frame = 0

Query: 1   MEHFNIEERISITIDDYESLGSGNYEGDEYDSLTSNRFRFLQASGMTRLEDKGVEHQTVA 60
           ME   +EE I  TI+DYE LGSGN+ GDEYDSL+SNRFR LQ SGM RLE+KG+EH+TVA
Sbjct: 1   MEQSIVEEGIFTTIEDYERLGSGNH-GDEYDSLSSNRFRLLQGSGMRRLEEKGLEHETVA 60

Query: 61  KSFVSGLGLLGRDARVVAVHKNWSSDSGKTARAEAFRIFSDAVAAKCGGDANIKFAWYGA 120
           K FVSGLG +GRDA+VVAVHKNWSSDSGK AR EAFRIFSDAVA KCGGDANIKF WYGA
Sbjct: 61  KCFVSGLGSIGRDAQVVAVHKNWSSDSGKVAREEAFRIFSDAVATKCGGDANIKFGWYGA 120

Query: 121 PIDEICDIVSHGFSHFSHNNNDADSESSHGVGVHLSPGRFSIDCVSSSAADRNGLRHMLL 180
           P+D+IC IVSHGFS F+H   D  SES HGVGV L   RFSIDCVSSSA D+NGL+H LL
Sbjct: 121 PVDDICQIVSHGFSRFNH--KDMRSESLHGVGVSLCAARFSIDCVSSSAVDQNGLQHALL 180

Query: 181 CRVILGKVEAIPVGSTQSHPTSSAFDTAVDSLFAPRKYIVWAAFMNSHIQPAYVVSFKAP 240
           CRVILGKVEAIP GS Q +P+SSAFDT VDSLFAPRKYIVW AFMNSHIQP YVVSFK P
Sbjct: 181 CRVILGKVEAIPAGSKQFYPSSSAFDTGVDSLFAPRKYIVWDAFMNSHIQPTYVVSFKTP 240

Query: 241 DPNDAQMN--SVSVMKPTSPWMSFPTLMSALSRFLDPGRMSLIQKCHKDFVRNKIPRPQL 300
            P  AQMN  SV+++KP S W+SFPTLM  LSRFLDP +MSLI K H+DF+RNKIPRPQL
Sbjct: 241 QPKGAQMNMKSVNMVKPRSAWVSFPTLMLTLSRFLDPRKMSLIHKWHEDFIRNKIPRPQL 300

Query: 301 INRVRHIAGDELLIALIKKHRRNEVMPLA 328
           IN+VRHIAGDELLIA+IKK RR EVM +A
Sbjct: 301 INKVRHIAGDELLIAVIKKQRRKEVMQVA 326

BLAST of Lag0005319 vs. NCBI nr
Match: XP_023538139.1 (probable inactive poly [ADP-ribose] polymerase SRO2 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 492.3 bits (1266), Expect = 3.3e-135
Identity = 249/329 (75.68%), Postives = 275/329 (83.59%), Query Frame = 0

Query: 1   MEHFNIEERISITIDDYESLGSGNYEGDEYDSLTSNRFRFLQASGMTRLEDKGVEHQTVA 60
           ME  N+EE I  TI+DYE LGSGN+ GDEYDSL+SNRFR LQ SGM  LE+KG+EH+TVA
Sbjct: 1   MEQSNVEEGIFTTIEDYERLGSGNH-GDEYDSLSSNRFRLLQGSGMRMLEEKGLEHETVA 60

Query: 61  KSFVSGLGLLGRDARVVAVHKNWSSDSGKTARAEAFRIFSDAVAAKCGGDANIKFAWYGA 120
           K FVSGLG +GRDA+VVAVHKNWSSDSGK  R EAFRIFSDAVA KCGGDANIKF WYGA
Sbjct: 61  KCFVSGLGSIGRDAQVVAVHKNWSSDSGKVGREEAFRIFSDAVATKCGGDANIKFGWYGA 120

Query: 121 PIDEICDIVSHGFSHFSHNNNDADSESSHGVGVHLSPGRFSIDCVSSSAADRNGLRHMLL 180
           P+D+IC IVSHGFS F+H   D  SES HGVGV L   RFSIDCVSSSA D+NGL+H LL
Sbjct: 121 PVDDICQIVSHGFSRFNH--KDMRSESLHGVGVSLCAARFSIDCVSSSAVDQNGLQHALL 180

Query: 181 CRVILGKVEAIPVGSTQSHPTSSAFDTAVDSLFAPRKYIVWAAFMNSHIQPAYVVSFKAP 240
           CRVILGKVEAIP GS Q +P+SSAFDT VDSLFAPRKYIVW AFMNSHIQP YVVSFKAP
Sbjct: 181 CRVILGKVEAIPAGSKQFYPSSSAFDTGVDSLFAPRKYIVWDAFMNSHIQPTYVVSFKAP 240

Query: 241 DPNDAQMN--SVSVMKPTSPWMSFPTLMSALSRFLDPGRMSLIQKCHKDFVRNKIPRPQL 300
            P  AQMN  SV+++KP S W+SFPTLM  LSRFLDP +MSLI K H+DF+RNKIPRPQL
Sbjct: 241 QPKGAQMNMKSVNMVKPRSAWVSFPTLMLTLSRFLDPRKMSLIHKWHEDFIRNKIPRPQL 300

Query: 301 INRVRHIAGDELLIALIKKHRRNEVMPLA 328
           IN+VRHIAGDELLIA+IK  RR EVM +A
Sbjct: 301 INKVRHIAGDELLIAVIKNQRRKEVMQVA 326

BLAST of Lag0005319 vs. NCBI nr
Match: KAG6585158.1 (putative inactive poly [ADP-ribose] polymerase SRO2, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 489.6 bits (1259), Expect = 2.1e-134
Identity = 249/329 (75.68%), Postives = 276/329 (83.89%), Query Frame = 0

Query: 1   MEHFNIEERISITIDDYESLGSGNYEGDEYDSLTSNRFRFLQASGMTRLEDKGVEHQTVA 60
           ME  N+EE I  TI+DYE LGSGN+ GDEYDSL+SNRFR LQ SGM RLE+KG+EH+TVA
Sbjct: 1   MEQSNVEEGIFTTIEDYERLGSGNH-GDEYDSLSSNRFRLLQGSGMRRLEEKGLEHETVA 60

Query: 61  KSFVSGLGLLGRDARVVAVHKNWSSDSGKTARAEAFRIFSDAVAAKCGGDANIKFAWYGA 120
           K F+SGLG +GRDA+VVAVHKNWSSDSGK AR EAFRIFSDAVA KCGGDANIKF WYGA
Sbjct: 61  KCFLSGLGSIGRDAQVVAVHKNWSSDSGKVAREEAFRIFSDAVATKCGGDANIKFGWYGA 120

Query: 121 PIDEICDIVSHGFSHFSHNNNDADSESSHGVGVHLSPGRFSIDCVSSSAADRNGLRHMLL 180
           P+D+IC IVSHGFS F+H   D  SE  HGVGV L   RFSIDCVSSSA D+NGL+H LL
Sbjct: 121 PVDDICQIVSHGFSRFNH--KDMRSE-LHGVGVSLCAARFSIDCVSSSAVDQNGLQHALL 180

Query: 181 CRVILGKVEAIPVGSTQSHPTSSAFDTAVDSLFAPRKYIVWAAFMNSHIQPAYVVSFKAP 240
           CRVILGKVEAIP GS Q +P+SSAFDT VDSLFAPRKYIVW AFMNSHIQP YVVSFKAP
Sbjct: 181 CRVILGKVEAIPAGSKQFYPSSSAFDTGVDSLFAPRKYIVWDAFMNSHIQPTYVVSFKAP 240

Query: 241 DPNDAQMN--SVSVMKPTSPWMSFPTLMSALSRFLDPGRMSLIQKCHKDFVRNKIPRPQL 300
            P   QMN  SV+++KP S W+SFPTLM  LSRFLDP +MSLI K H+DF+RNKIPRPQL
Sbjct: 241 QPKGTQMNMKSVNMVKPRSAWVSFPTLMLTLSRFLDPRKMSLIHKWHEDFIRNKIPRPQL 300

Query: 301 INRVRHIAGDELLIALIKKHRRNEVMPLA 328
           IN+VRHIAGDELLIA+IKK RR EVM +A
Sbjct: 301 INKVRHIAGDELLIAVIKKQRRKEVMQVA 325

BLAST of Lag0005319 vs. NCBI nr
Match: XP_023002607.1 (probable inactive poly [ADP-ribose] polymerase SRO2 [Cucurbita maxima])

HSP 1 Score: 479.9 bits (1234), Expect = 1.7e-131
Identity = 246/329 (74.77%), Postives = 274/329 (83.28%), Query Frame = 0

Query: 1   MEHFNIEERISITIDDYESLGSGNYEGDEYDSLTSNRFRFLQASGMTRLEDKGVEHQTVA 60
           ME  N+EE I  TI+DYE LGSGN+ GDEYDSL+SNRFR LQ SGM RLE++G+EH+TVA
Sbjct: 1   MEQSNVEEGIFTTIEDYERLGSGNH-GDEYDSLSSNRFRLLQGSGMRRLEEQGLEHETVA 60

Query: 61  KSFVSGLGLLGRDARVVAVHKNWSSDSGKTARAEAFRIFSDAVAAKCGGDANIKFAWYGA 120
             FVSGLG +GRDARVVAVHKN  SDSGK AR EAFRIFSDAVA KCGGDANIKF WYGA
Sbjct: 61  NCFVSGLGSIGRDARVVAVHKN-CSDSGKVAREEAFRIFSDAVATKCGGDANIKFGWYGA 120

Query: 121 PIDEICDIVSHGFSHFSHNNNDADSESSHGVGVHLSPGRFSIDCVSSSAADRNGLRHMLL 180
           P+D+IC IVS+GFS F+H   D  SES HGVGV L   RFSIDCVSSSA D+NGL+H LL
Sbjct: 121 PVDDICQIVSYGFSRFNH--KDMRSESLHGVGVSLCAARFSIDCVSSSAVDQNGLQHALL 180

Query: 181 CRVILGKVEAIPVGSTQSHPTSSAFDTAVDSLFAPRKYIVWAAFMNSHIQPAYVVSFKAP 240
           CRVILGKVEAIP GS Q +P+SSAFDT VDSLF PRKYIVW AF+NSHIQP YVVSFKAP
Sbjct: 181 CRVILGKVEAIPAGSKQFYPSSSAFDTGVDSLFVPRKYIVWDAFVNSHIQPTYVVSFKAP 240

Query: 241 DPNDAQMN--SVSVMKPTSPWMSFPTLMSALSRFLDPGRMSLIQKCHKDFVRNKIPRPQL 300
            P  AQMN  SV+V+KP S W+SFPTLM  LSRFLDP +MSLI K H+DF+RNKIPRPQL
Sbjct: 241 RPKGAQMNMKSVNVVKPRSAWVSFPTLMLTLSRFLDPRKMSLIHKWHEDFIRNKIPRPQL 300

Query: 301 INRVRHIAGDELLIALIKKHRRNEVMPLA 328
           IN+VRH+AGDELLIA+IKK RR EVM +A
Sbjct: 301 INKVRHVAGDELLIAVIKKQRRKEVMQVA 325

BLAST of Lag0005319 vs. NCBI nr
Match: KAG7020078.1 (putative inactive poly [ADP-ribose] polymerase SRO2, partial [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 357.8 bits (917), Expect = 9.7e-95
Identity = 184/242 (76.03%), Postives = 200/242 (82.64%), Query Frame = 0

Query: 1   MEHFNIEERISITIDDYESLGSGNYEGDEYDSLTSNRFRFLQASGMTRLEDKGVEHQTVA 60
           ME  N+EE I  TI+DYE LGSGN+ GDEYDSL+SNRFR LQ SGM RLE+KG+EH+TVA
Sbjct: 1   MEQSNVEEGIFTTIEDYERLGSGNH-GDEYDSLSSNRFRLLQGSGMRRLEEKGLEHETVA 60

Query: 61  KSFVSGLGLLGRDARVVAVHKNWSSDSGKTARAEAFRIFSDAVAAKCGGDANIKFAWYGA 120
           K FVSGLG +GRDA+VVAVHKNWSSDSGK AR EAFRIFSDAVA KCGGDANIKF WYGA
Sbjct: 61  KCFVSGLGSIGRDAQVVAVHKNWSSDSGKVAREEAFRIFSDAVATKCGGDANIKFGWYGA 120

Query: 121 PIDEICDIVSHGFSHFSHNNNDADSESSHGVGVHLSPGRFSIDCVSSSAADRNGLRHMLL 180
           P+D+IC IVSHGFS F+H   D  SE  HGVGV L   RFSIDCVSSSA D+NGL+H   
Sbjct: 121 PVDDICQIVSHGFSRFNH--KDMRSE-LHGVGVSLCAARFSIDCVSSSAVDQNGLQH--- 180

Query: 181 CRVILGKVEAIPVGSTQSHPTSSAFDTAVDSLFAPRKYIVWAAFMNSHIQPAYVVSFKAP 240
             VILGKVEAIP GS Q +P+SSAFDT VDSLFAPRKYIVW AFMNSHIQP YVVSFKAP
Sbjct: 181 -AVILGKVEAIPAGSKQFYPSSSAFDTGVDSLFAPRKYIVWDAFMNSHIQPTYVVSFKAP 234

Query: 241 DP 243
            P
Sbjct: 241 QP 234

BLAST of Lag0005319 vs. ExPASy Swiss-Prot
Match: Q9ZUD9 (Probable inactive poly [ADP-ribose] polymerase SRO2 OS=Arabidopsis thaliana OX=3702 GN=SRO2 PE=1 SV=1)

HSP 1 Score: 248.8 bits (634), Expect = 8.3e-65
Identity = 141/322 (43.79%), Postives = 204/322 (63.35%), Query Frame = 0

Query: 6   IEERISIT-IDDYESLGSGNYEGDEYDSLTSNRFRFLQASGMTRLEDKGVEHQTVAKSFV 65
           IE++ S+T +D+ E   S     D+ DS  S+      +S +  L +   EH  +    +
Sbjct: 7   IEDQTSVTNLDNGEIFDS---ISDDADSSVSHAGSSFSSSSLILLGEGNPEHDVIKTCLL 66

Query: 66  SGLGLLGRDARVVAVHKNWSSDSGKTARAE--AFRIFSDAVAAKCGGDANIKFAWYGAPI 125
           SG+G++  D  +V + KN SS+ G T RA+  AFRIF+DAVA K GGDAN+K+ WY    
Sbjct: 67  SGMGVVSSDTTIVTISKN-SSERGITTRAKFLAFRIFTDAVARKHGGDANVKYGWYAGSR 126

Query: 126 DEICDIVSHGFSHFSHNNNDADSESSHGVGVHLSPGRFSIDCVSSSAADRNGLRHMLLCR 185
           DEI  I+S+GFS+      + D   SHG+G+HL P + S+   S++  D  GLR++LLCR
Sbjct: 127 DEIQRIISYGFSNRDVGKFENDG-GSHGIGIHLVPSKCSLLAASATEQDEEGLRYLLLCR 186

Query: 186 VILGKVEAIPVGSTQSHPTSSAFDTAVDSLFAPRKYIVWAAFMNSHIQPAYVVSFKAPDP 245
           VILGK E I  GS QS+P+S+ FD+ VD L  PR Y++W+  MNS I P+Y+VSF++P  
Sbjct: 187 VILGKPEIIISGSKQSYPSSAEFDSGVDDLHNPRNYVIWSCNMNSCILPSYIVSFRSPRL 246

Query: 246 NDAQMNSVSVMKPTSPWMSFPTLMSALSRFLDPGRMSLIQKCHKDFVRNKIPRPQLINRV 305
             ++    S  +P+SPW+SF +LMS LS  +DP RM+LI + + DF + KI R QL+ ++
Sbjct: 247 RVSRGGFAS--RPSSPWVSFASLMSMLSTSMDPSRMNLIIRTYDDFRKRKIRRDQLVRKM 306

Query: 306 RHIAGDELLIALIKKHR-RNEV 324
           R +AGD LL  +IK H+ RN+V
Sbjct: 307 REVAGDNLLAEIIKNHKNRNKV 321

BLAST of Lag0005319 vs. ExPASy Swiss-Prot
Match: O64592 (Probable inactive poly [ADP-ribose] polymerase SRO3 OS=Arabidopsis thaliana OX=3702 GN=SRO3 PE=1 SV=2)

HSP 1 Score: 195.3 bits (495), Expect = 1.1e-48
Identity = 112/310 (36.13%), Postives = 173/310 (55.81%), Query Frame = 0

Query: 12  ITIDDYESLGSGNYEGDEYDSLTSNRFRFLQASGMTRLEDKGVEHQTVAKSFVSGLGLLG 71
           + I+D ES+   N +  E  +  S+            L +   EH  +   F+SG+G   
Sbjct: 5   VEIEDQESV--TNLDNGEIINPISDNAPNFSGDATILLREATFEHNLIKNCFLSGMGSFA 64

Query: 72  RDARVVAVHKNWSSDSGKT-ARAEAFRIFSDAVAAKCGGDANIKFAWYGAPIDEICDIVS 131
            +  +V V K  +     T A+   F++F++A+  K  G ANI++ WY    +EI  +++
Sbjct: 65  TETTIVTVRKILTQRLITTKAKFAVFKLFTEAMKRKNNGYANIRYGWYSGSKEEIDRVIT 124

Query: 132 HGFSHFSHNNNDADSESSHGVGVHLSPGRFSIDCVSSSAADRNGLRHMLLCRVILGKVEA 191
           +GFS+      + D   SHGVG+HL   R+S+        D  G++++LLCRVILGK E 
Sbjct: 125 YGFSNREIKKVEND-VGSHGVGIHLVHHRYSLAAALVGEGDEEGIKNILLCRVILGKPEQ 184

Query: 192 IPVGSTQSHPTSSAFDTAVDSLFAPRKYIVWAAFMNSHIQPAYVVSFKAPDPNDAQMNSV 251
           I  GS QS+P+S+ FD+ VD+L  PRKY++W+  MNS+I P Y+VSFK      + +   
Sbjct: 185 IVTGSKQSYPSSNQFDSGVDNLENPRKYVIWSCNMNSYILPTYIVSFK------SHLLRG 244

Query: 252 SVMKPTSPWMSFPTLMSALSRFLDPGRMSLIQKCHKDFVRNKIPRPQLINRVRHIAGDEL 311
            + +  SP +SF  LMS LS+ LD  RM+LI   + DF + K+ R QL+ ++R + GD L
Sbjct: 245 LIGRARSPCVSFSVLMSILSKSLDAARMNLILTSYDDFRKRKLRREQLVRKIREVVGDNL 304

Query: 312 LIALIKKHRR 321
           L  ++K  RR
Sbjct: 305 LFKILKNQRR 305

BLAST of Lag0005319 vs. ExPASy Swiss-Prot
Match: Q9FJJ3 (Probable inactive poly [ADP-ribose] polymerase SRO5 OS=Arabidopsis thaliana OX=3702 GN=SRO5 PE=1 SV=1)

HSP 1 Score: 194.5 bits (493), Expect = 1.9e-48
Identity = 118/315 (37.46%), Postives = 178/315 (56.51%), Query Frame = 0

Query: 6   IEERISITIDDYESLGSGNYEGDEYDSLTSNRFRFLQASGMTRLEDKGVEHQTVAKSFVS 65
           +  ++    DD E  GS   E    DS  S+   F    G+  L +    H  + ++  S
Sbjct: 4   VRTQVEAVFDDSEQDGSTISESGSCDS--SSDRSFADELGLMELLEGDKAHDLIYRNCKS 63

Query: 66  GLGLLGRDARVVAVHKNWSSDSGKTARAEAFRIFSDAVAAKCGGD--ANIKFAWYGAPID 125
           G   LG   ++++V +N   + G  A+ + F++F +AV  K GGD  A +K+ W      
Sbjct: 64  G---LGDQCQILSVLRNGFRNVGSRAKLKTFQVFQEAVQMKHGGDGGAKVKYGWCSVSKH 123

Query: 126 EICDIVSHGFSHFSHNNNDADSESSHGVGVHLSPGRFSIDCVSSSAAD-RNGLRHMLLCR 185
           E+  I  +GFS    N      + S G G++LSP    +DC+  SA++  +G+R +LLCR
Sbjct: 124 ELKTIFEYGFSEPLRN------DGSFGRGLYLSPDNSPLDCLKDSASESEDGMRFLLLCR 183

Query: 186 VILGKVEAIPVGSTQSHPTSSAFDTAVDSLFAPRKYIVWAAFMNSHIQPAYVVSFKAPDP 245
           V+LGK E +P GST+S P+S  FD+ VD L + +KYIVW+  MN+H+ P ++V  KAP  
Sbjct: 184 VLLGKSEIVPQGSTRSCPSSPEFDSGVDDLVSTKKYIVWSTHMNTHVLPEFLVCIKAP-- 243

Query: 246 NDAQMN-SVSVMKPTSPWMSFPTLMSALSRFLDPGRMSLIQKCHKDFVRNKIPRPQLINR 305
                N + S  +  SPWM+FP L+ ALS+FL P ++ +IQK +KD    +I R +LI R
Sbjct: 244 ----FNLTRSPKRLRSPWMAFPVLIKALSKFLPPSQILVIQKHYKDQQNRRITRSELIQR 301

Query: 306 VRHIAGDELLIALIK 317
           VR I GD+LL+ +IK
Sbjct: 304 VRSITGDKLLVHIIK 301

BLAST of Lag0005319 vs. ExPASy Swiss-Prot
Match: Q9STU1 (Probable inactive poly [ADP-ribose] polymerase SRO4 OS=Arabidopsis thaliana OX=3702 GN=SRO4 PE=2 SV=2)

HSP 1 Score: 144.1 bits (362), Expect = 2.9e-33
Identity = 97/305 (31.80%), Postives = 160/305 (52.46%), Query Frame = 0

Query: 28  DEYDSLTSNRFRFLQASGMTRLEDKGVEHQTVAKSFVSGL-GLLGRDARVVAVHKNWSSD 87
           D+  S  +N F      G+T L      ++ + +   S L   L     +V++ KN    
Sbjct: 34  DQQHSSIANEF------GLTELPKDDKVYELIYRHCQSKLTSHLSNQFEIVSILKNGFQT 93

Query: 88  SGKTARAEAFRIFSDAVAAKCGG----------DANIKFAWYGAPIDEICDIVSHGFSHF 147
               A+ +AF+I++++VA K G            A +K+   G   +E+  I+ +GFS  
Sbjct: 94  PLGQAKLKAFQIYAESVAKKSGSCCGNKAAVAEAARVKYGCCGVEKEELKAILMYGFS-- 153

Query: 148 SHNNNDADSESSHGVGVHLSPGRFSIDCV--SSSAADRNGLRHMLLCRVILGKVEAIPVG 207
              NN           + LSP    + C+   SS+ + +G+  +L  R+I+GK E +   
Sbjct: 154 ---NN----------ALCLSPDNAPLQCMIDPSSSCNEDGISFLLFSRIIMGKSEVV-CS 213

Query: 208 STQSHPTSSAFDTAVDSLFAPRKYIVWAAFMNSHIQPAYVVSFKAPDPNDAQMNSVSVMK 267
           ++QS+P+S  FD+ VDSL +P KYI+W+  MN+H+ P +VV  K P          S++K
Sbjct: 214 TSQSYPSSMEFDSGVDSLTSPNKYIIWSTHMNTHVLPEFVVCIKTP----------SILK 273

Query: 268 ---PTSPWMSFPTLMSALSRFLDPGRMSLIQKCHKDFVRNKIPRPQLINRVRHIAGDELL 317
              P SPW+SFP L++++S+FL+  ++ LI K +K+    +I R +LI R+R I GD LL
Sbjct: 274 RKNPKSPWISFPVLINSISKFLNQSQIRLIHKHYKEHQDRRISRCELIQRLRSITGDSLL 306

BLAST of Lag0005319 vs. ExPASy Swiss-Prot
Match: O82289 (Probable inactive poly [ADP-ribose] polymerase SRO1 OS=Arabidopsis thaliana OX=3702 GN=SRO1 PE=1 SV=2)

HSP 1 Score: 113.2 bits (282), Expect = 5.5e-24
Identity = 106/369 (28.73%), Postives = 167/369 (45.26%), Query Frame = 0

Query: 1   MEHFNIEERISITIDDYESLGSGNYEGDE----YDSLTSNRFRFLQASGMTRLEDKGVEH 60
           M+ F   +R S   +D  S  S + E D+    +D   ++RF     SG+   E++ ++ 
Sbjct: 218 MDDFQAVQRSSNGPNDEASEDSCSRELDDAVEKWDKTETDRF-----SGVKPAEEE-LDK 277

Query: 61  QTVAKSFVSGLGLLGRDARVVAVHKNWSSDSGKTARAEAFRIFSDAVAAKCGGDANIKFA 120
             V + F  G   LG     + V++ +SS+  K AR   F+  +D +  K  GDANI++A
Sbjct: 278 DAVKQMFALGAATLGH-VESLDVYQ-FSSEIAK-ARLSLFQKQAD-ITKKHRGDANIRYA 337

Query: 121 WYGAPIDEICDIVSHGFSHFSHNNNDADSESSHGVGVHLSPGRFSIDCVSSSA----ADR 180
           W  A  + +  ++ HG             +S +GVGVH      + +C   SA     D 
Sbjct: 338 WVPAKKEVLSAVMMHGLG----VGGAFIKKSMYGVGVH------AANCPYFSARYCDIDD 397

Query: 181 NGLRHMLLCRVILGKVEAIPVGSTQSHPTSSAFDTAVDSLFAPRKYIVWAAFMNSHIQPA 240
           NG+RHM+LCRVI+G +E +   +TQ       +D  VD + +P+ Y++W   MN+HI P 
Sbjct: 398 NGVRHMVLCRVIMGNMEPLRGDNTQYFTGGEEYDNGVDDVESPKHYLIWNMNMNTHIYPE 457

Query: 241 YVVSFKAPDPN---------------------------------------DAQMNSVSVM 300
           +VVSFK   PN                                        ++ NS S  
Sbjct: 458 FVVSFKLSIPNAEGNILPTTQSRHESSGLTLEGPKGSPSNEPGRVSNGGSGSEKNSSSSR 517

Query: 301 KPTSPWMSFPTLMSALSRFLDPGRMSLIQKCHKDFVRNKIPRPQLINRVRHIAG-DELLI 322
           +P SP M FP L  A+S  +    M LI   +++    K+ R +    +  I G D+LLI
Sbjct: 518 RPRSPIMPFPLLFKAISSKIARKDMDLIIAGYQELREKKVSRKEFYKTLSMIVGDDDLLI 566

BLAST of Lag0005319 vs. ExPASy TrEMBL
Match: A0A6J1GGW9 (probable inactive poly [ADP-ribose] polymerase SRO2 OS=Cucurbita moschata OX=3662 GN=LOC111454113 PE=4 SV=1)

HSP 1 Score: 493.0 bits (1268), Expect = 9.4e-136
Identity = 250/329 (75.99%), Postives = 276/329 (83.89%), Query Frame = 0

Query: 1   MEHFNIEERISITIDDYESLGSGNYEGDEYDSLTSNRFRFLQASGMTRLEDKGVEHQTVA 60
           ME   +EE I  TI+DYE LGSGN+ GDEYDSL+SNRFR LQ SGM RLE+KG+EH+TVA
Sbjct: 1   MEQSIVEEGIFTTIEDYERLGSGNH-GDEYDSLSSNRFRLLQGSGMRRLEEKGLEHETVA 60

Query: 61  KSFVSGLGLLGRDARVVAVHKNWSSDSGKTARAEAFRIFSDAVAAKCGGDANIKFAWYGA 120
           K FVSGLG +GRDA+VVAVHKNWSSDSGK AR EAFRIFSDAVA KCGGDANIKF WYGA
Sbjct: 61  KCFVSGLGSIGRDAQVVAVHKNWSSDSGKVAREEAFRIFSDAVATKCGGDANIKFGWYGA 120

Query: 121 PIDEICDIVSHGFSHFSHNNNDADSESSHGVGVHLSPGRFSIDCVSSSAADRNGLRHMLL 180
           P+D+IC IVSHGFS F+H   D  SES HGVGV L   RFSIDCVSSSA D+NGL+H LL
Sbjct: 121 PVDDICQIVSHGFSRFNH--KDMRSESLHGVGVSLCAARFSIDCVSSSAVDQNGLQHALL 180

Query: 181 CRVILGKVEAIPVGSTQSHPTSSAFDTAVDSLFAPRKYIVWAAFMNSHIQPAYVVSFKAP 240
           CRVILGKVEAIP GS Q +P+SSAFDT VDSLFAPRKYIVW AFMNSHIQP YVVSFK P
Sbjct: 181 CRVILGKVEAIPAGSKQFYPSSSAFDTGVDSLFAPRKYIVWDAFMNSHIQPTYVVSFKTP 240

Query: 241 DPNDAQMN--SVSVMKPTSPWMSFPTLMSALSRFLDPGRMSLIQKCHKDFVRNKIPRPQL 300
            P  AQMN  SV+++KP S W+SFPTLM  LSRFLDP +MSLI K H+DF+RNKIPRPQL
Sbjct: 241 QPKGAQMNMKSVNMVKPRSAWVSFPTLMLTLSRFLDPRKMSLIHKWHEDFIRNKIPRPQL 300

Query: 301 INRVRHIAGDELLIALIKKHRRNEVMPLA 328
           IN+VRHIAGDELLIA+IKK RR EVM +A
Sbjct: 301 INKVRHIAGDELLIAVIKKQRRKEVMQVA 326

BLAST of Lag0005319 vs. ExPASy TrEMBL
Match: A0A6J1KJZ7 (probable inactive poly [ADP-ribose] polymerase SRO2 OS=Cucurbita maxima OX=3661 GN=LOC111496418 PE=4 SV=1)

HSP 1 Score: 479.9 bits (1234), Expect = 8.2e-132
Identity = 246/329 (74.77%), Postives = 274/329 (83.28%), Query Frame = 0

Query: 1   MEHFNIEERISITIDDYESLGSGNYEGDEYDSLTSNRFRFLQASGMTRLEDKGVEHQTVA 60
           ME  N+EE I  TI+DYE LGSGN+ GDEYDSL+SNRFR LQ SGM RLE++G+EH+TVA
Sbjct: 1   MEQSNVEEGIFTTIEDYERLGSGNH-GDEYDSLSSNRFRLLQGSGMRRLEEQGLEHETVA 60

Query: 61  KSFVSGLGLLGRDARVVAVHKNWSSDSGKTARAEAFRIFSDAVAAKCGGDANIKFAWYGA 120
             FVSGLG +GRDARVVAVHKN  SDSGK AR EAFRIFSDAVA KCGGDANIKF WYGA
Sbjct: 61  NCFVSGLGSIGRDARVVAVHKN-CSDSGKVAREEAFRIFSDAVATKCGGDANIKFGWYGA 120

Query: 121 PIDEICDIVSHGFSHFSHNNNDADSESSHGVGVHLSPGRFSIDCVSSSAADRNGLRHMLL 180
           P+D+IC IVS+GFS F+H   D  SES HGVGV L   RFSIDCVSSSA D+NGL+H LL
Sbjct: 121 PVDDICQIVSYGFSRFNH--KDMRSESLHGVGVSLCAARFSIDCVSSSAVDQNGLQHALL 180

Query: 181 CRVILGKVEAIPVGSTQSHPTSSAFDTAVDSLFAPRKYIVWAAFMNSHIQPAYVVSFKAP 240
           CRVILGKVEAIP GS Q +P+SSAFDT VDSLF PRKYIVW AF+NSHIQP YVVSFKAP
Sbjct: 181 CRVILGKVEAIPAGSKQFYPSSSAFDTGVDSLFVPRKYIVWDAFVNSHIQPTYVVSFKAP 240

Query: 241 DPNDAQMN--SVSVMKPTSPWMSFPTLMSALSRFLDPGRMSLIQKCHKDFVRNKIPRPQL 300
            P  AQMN  SV+V+KP S W+SFPTLM  LSRFLDP +MSLI K H+DF+RNKIPRPQL
Sbjct: 241 RPKGAQMNMKSVNVVKPRSAWVSFPTLMLTLSRFLDPRKMSLIHKWHEDFIRNKIPRPQL 300

Query: 301 INRVRHIAGDELLIALIKKHRRNEVMPLA 328
           IN+VRH+AGDELLIA+IKK RR EVM +A
Sbjct: 301 INKVRHVAGDELLIAVIKKQRRKEVMQVA 325

BLAST of Lag0005319 vs. ExPASy TrEMBL
Match: A0A5B7AWT5 (Poly [ADP-ribose] polymerase OS=Davidia involucrata OX=16924 GN=Din_030016 PE=4 SV=1)

HSP 1 Score: 334.0 bits (855), Expect = 7.3e-88
Identity = 165/323 (51.08%), Postives = 222/323 (68.73%), Query Frame = 0

Query: 1   MEHFNIEERISITIDDYESLGSGNYEGDEYDSLTSNRFRFLQASGMTRLEDKGVEHQTVA 60
           M+H  IEE++S+T+DD+E + S + E D   S +S +FRFL   GM ++E+   EH+ + 
Sbjct: 1   MDHIGIEEQVSMTVDDHE-ISSADSEIDSSASSSSEQFRFLTQKGMIQVEEGNSEHEIIK 60

Query: 61  KSFVSGLGLLGRDARVVAVHKNWSSDSGKTARAEAFRIFSDAVAAKCGGDANIKFAWYGA 120
           KSF+ G+G LG+D  +VA+H+N  S     AR E FRIFS+AVA KCGG+ANIK AWYGA
Sbjct: 61  KSFLEGIGPLGKDTNIVAIHRNSHSCLTGKARLETFRIFSEAVAKKCGGNANIKHAWYGA 120

Query: 121 PIDEICDIVSHGFSHFSHNNNDADSESSHGVGVHLSPGRFSIDCVSSSAADRNGLRHMLL 180
             DE+C+I+SHGFS         ++   +G+GV+LSP  FSID  SS+  D NGLRH+LL
Sbjct: 121 SRDEVCEIISHGFSQC----RPPENRELYGLGVYLSPAIFSIDGASSAVVDENGLRHVLL 180

Query: 181 CRVILGKVEAIPVGSTQSHPTSSAFDTAVDSLFAPRKYIVWAAFMNSHIQPAYVVSFKAP 240
           CRVILG +E +  GS QS P+S  FD+ VD L AP++YI+W+ +MNSHI P Y++SF+AP
Sbjct: 181 CRVILGNMEVVCAGSEQSQPSSKEFDSGVDDLSAPKRYIIWSPYMNSHILPNYIISFRAP 240

Query: 241 DPNDAQMNSVSVMKPTSPWMSFPTLMSALSRFLDPGRMSLIQKCHKDFVRNKIPRPQLIN 300
               +Q     +MKP SP+M  PTLMS LS FL P +M LI K H DF  NKI RP+L+ 
Sbjct: 241 CSRGSQRIQAPIMKPNSPYMKLPTLMSILSGFLPPSKMILISKYHSDFQENKITRPRLVQ 300

Query: 301 RVRHIAGDELLIALIKKHRRNEV 324
            +R +AGD+LL A+IK HR  ++
Sbjct: 301 MLRQLAGDKLLTAVIKLHRNKKL 318

BLAST of Lag0005319 vs. ExPASy TrEMBL
Match: A0A5B7AX47 (Poly [ADP-ribose] polymerase OS=Davidia involucrata OX=16924 GN=Din_030019 PE=4 SV=1)

HSP 1 Score: 333.6 bits (854), Expect = 9.5e-88
Identity = 165/323 (51.08%), Postives = 222/323 (68.73%), Query Frame = 0

Query: 1   MEHFNIEERISITIDDYESLGSGNYEGDEYDSLTSNRFRFLQASGMTRLEDKGVEHQTVA 60
           M+H  IEE++S+T+DD+E + S + E D   S +S +FRFL   GM ++E+   EH+ + 
Sbjct: 1   MDHIGIEEQVSMTVDDHE-ISSADSEIDSSASSSSEQFRFLTQKGMIQVEEGNSEHEIIK 60

Query: 61  KSFVSGLGLLGRDARVVAVHKNWSSDSGKTARAEAFRIFSDAVAAKCGGDANIKFAWYGA 120
           KSF+ G+G LG+D  +VA+H+N  S     AR E FRIFS+AVA KCGG+ANIK AWYGA
Sbjct: 61  KSFLEGIGPLGKDTNIVAIHRNSHSCLTGKARLETFRIFSEAVAKKCGGNANIKHAWYGA 120

Query: 121 PIDEICDIVSHGFSHFSHNNNDADSESSHGVGVHLSPGRFSIDCVSSSAADRNGLRHMLL 180
             DE+C+I+SHGFS         ++   +G+GV+LSP  FSID  SS+  D NGLRH+LL
Sbjct: 121 SRDEVCEIISHGFSQC----RPPENRELYGLGVYLSPAIFSIDGASSAVVDENGLRHVLL 180

Query: 181 CRVILGKVEAIPVGSTQSHPTSSAFDTAVDSLFAPRKYIVWAAFMNSHIQPAYVVSFKAP 240
           CRVILG +E +  GS QS P+S  FD+ VD L AP++YI+W+ +MNSHI P Y++SF+AP
Sbjct: 181 CRVILGNMEVVCAGSEQSQPSSKEFDSGVDDLSAPKRYIIWSPYMNSHILPNYIISFRAP 240

Query: 241 DPNDAQMNSVSVMKPTSPWMSFPTLMSALSRFLDPGRMSLIQKCHKDFVRNKIPRPQLIN 300
               +Q     +MKP SP+M  PTLMS LS FL P +M LI K H DF  NKI RP+L+ 
Sbjct: 241 CSRGSQRIRAPIMKPNSPYMKLPTLMSILSGFLPPSKMILISKYHSDFQENKITRPRLVQ 300

Query: 301 RVRHIAGDELLIALIKKHRRNEV 324
            +R +AGD+LL A+IK HR  ++
Sbjct: 301 MLRQLAGDKLLTAVIKLHRNKKL 318

BLAST of Lag0005319 vs. ExPASy TrEMBL
Match: A0A5N5G7K7 (Inactive poly [ADP-ribose] polymerase SRO2 OS=Pyrus ussuriensis x Pyrus communis OX=2448454 GN=D8674_037251 PE=4 SV=1)

HSP 1 Score: 332.8 bits (852), Expect = 1.6e-87
Identity = 175/327 (53.52%), Postives = 221/327 (67.58%), Query Frame = 0

Query: 2   EHFNIEERISITID-DYESLGSGNYEGDEYDSLTSNRFRFLQASGMTRLEDKGVEHQTVA 61
           + F  E++ISITID D E L SG+  GD  ++  S+RF     SGM R+ D+  EH+ + 
Sbjct: 4   DQFEFEDQISITIDNDEEILDSGSDCGDS-NAAVSDRFGVFTRSGMIRVGDESFEHEIIK 63

Query: 62  KSFVSGLGLLGRDARVVAVHKNWSSDSGKTARAEAFRIFSDAVAAKCGGDANIKFAWYGA 121
           KSFVSG+GL GRD  +VAVHKN SSD  + AR E+F+IFS AVA+KCGG+AN+K+AWYG 
Sbjct: 64  KSFVSGMGLAGRDTNIVAVHKNLSSDPTRKARFESFKIFSQAVASKCGGNANVKYAWYGG 123

Query: 122 PIDEICDIVSHGFSHFSHNNNDADSESSHGVGVHLSPGRFSIDCVSSSAADRNGLRHMLL 181
             +E+CD++ HGFS        A +E S+GVGVH+ P +F+ D   SSA D +GL+H+LL
Sbjct: 124 SKEELCDVLVHGFSRC---REPAPNEVSYGVGVHMIPAKFTCDGALSSAVDESGLKHILL 183

Query: 182 CRVILGKVEAIPVGSTQSHPTSSAFDTAVDSLFAPRKYIVWAAFMNSHIQPAYVVSFKAP 241
           CRVILGK E + +GS QS P+    DT VD+L  PR+Y+VW+A MNSHI P YVVSFK P
Sbjct: 184 CRVILGKAEMVALGSKQSQPSCKEVDTGVDNLVNPRRYVVWSAIMNSHIYPCYVVSFKVP 243

Query: 242 D--PN-----DAQMNSVSVMKPTSPWMSFPTLMSALSRFLDPGRMSLIQKCHKDFVRNKI 301
           +  PN          S     PTSPWMSFP LMS LS+FL P +M  I  CH +F  NK+
Sbjct: 244 NTLPNVVSGVPTAQQSALRQPPTSPWMSFPALMSILSKFLPPQKMQSIVACHNEFRANKV 303

Query: 302 PRPQLINRVRHIAGDELLIALIKKHRR 321
            RPQLI RVR IAGD LLI +IK  +R
Sbjct: 304 TRPQLIQRVRQIAGDRLLIGVIKSVKR 326

BLAST of Lag0005319 vs. TAIR 10
Match: AT1G23550.1 (similar to RCD one 2 )

HSP 1 Score: 248.8 bits (634), Expect = 5.9e-66
Identity = 141/322 (43.79%), Postives = 204/322 (63.35%), Query Frame = 0

Query: 6   IEERISIT-IDDYESLGSGNYEGDEYDSLTSNRFRFLQASGMTRLEDKGVEHQTVAKSFV 65
           IE++ S+T +D+ E   S     D+ DS  S+      +S +  L +   EH  +    +
Sbjct: 7   IEDQTSVTNLDNGEIFDS---ISDDADSSVSHAGSSFSSSSLILLGEGNPEHDVIKTCLL 66

Query: 66  SGLGLLGRDARVVAVHKNWSSDSGKTARAE--AFRIFSDAVAAKCGGDANIKFAWYGAPI 125
           SG+G++  D  +V + KN SS+ G T RA+  AFRIF+DAVA K GGDAN+K+ WY    
Sbjct: 67  SGMGVVSSDTTIVTISKN-SSERGITTRAKFLAFRIFTDAVARKHGGDANVKYGWYAGSR 126

Query: 126 DEICDIVSHGFSHFSHNNNDADSESSHGVGVHLSPGRFSIDCVSSSAADRNGLRHMLLCR 185
           DEI  I+S+GFS+      + D   SHG+G+HL P + S+   S++  D  GLR++LLCR
Sbjct: 127 DEIQRIISYGFSNRDVGKFENDG-GSHGIGIHLVPSKCSLLAASATEQDEEGLRYLLLCR 186

Query: 186 VILGKVEAIPVGSTQSHPTSSAFDTAVDSLFAPRKYIVWAAFMNSHIQPAYVVSFKAPDP 245
           VILGK E I  GS QS+P+S+ FD+ VD L  PR Y++W+  MNS I P+Y+VSF++P  
Sbjct: 187 VILGKPEIIISGSKQSYPSSAEFDSGVDDLHNPRNYVIWSCNMNSCILPSYIVSFRSPRL 246

Query: 246 NDAQMNSVSVMKPTSPWMSFPTLMSALSRFLDPGRMSLIQKCHKDFVRNKIPRPQLINRV 305
             ++    S  +P+SPW+SF +LMS LS  +DP RM+LI + + DF + KI R QL+ ++
Sbjct: 247 RVSRGGFAS--RPSSPWVSFASLMSMLSTSMDPSRMNLIIRTYDDFRKRKIRRDQLVRKM 306

Query: 306 RHIAGDELLIALIKKHR-RNEV 324
           R +AGD LL  +IK H+ RN+V
Sbjct: 307 REVAGDNLLAEIIKNHKNRNKV 321

BLAST of Lag0005319 vs. TAIR 10
Match: AT1G70440.1 (similar to RCD one 3 )

HSP 1 Score: 195.3 bits (495), Expect = 7.8e-50
Identity = 112/310 (36.13%), Postives = 173/310 (55.81%), Query Frame = 0

Query: 12  ITIDDYESLGSGNYEGDEYDSLTSNRFRFLQASGMTRLEDKGVEHQTVAKSFVSGLGLLG 71
           + I+D ES+   N +  E  +  S+            L +   EH  +   F+SG+G   
Sbjct: 5   VEIEDQESV--TNLDNGEIINPISDNAPNFSGDATILLREATFEHNLIKNCFLSGMGSFA 64

Query: 72  RDARVVAVHKNWSSDSGKT-ARAEAFRIFSDAVAAKCGGDANIKFAWYGAPIDEICDIVS 131
            +  +V V K  +     T A+   F++F++A+  K  G ANI++ WY    +EI  +++
Sbjct: 65  TETTIVTVRKILTQRLITTKAKFAVFKLFTEAMKRKNNGYANIRYGWYSGSKEEIDRVIT 124

Query: 132 HGFSHFSHNNNDADSESSHGVGVHLSPGRFSIDCVSSSAADRNGLRHMLLCRVILGKVEA 191
           +GFS+      + D   SHGVG+HL   R+S+        D  G++++LLCRVILGK E 
Sbjct: 125 YGFSNREIKKVEND-VGSHGVGIHLVHHRYSLAAALVGEGDEEGIKNILLCRVILGKPEQ 184

Query: 192 IPVGSTQSHPTSSAFDTAVDSLFAPRKYIVWAAFMNSHIQPAYVVSFKAPDPNDAQMNSV 251
           I  GS QS+P+S+ FD+ VD+L  PRKY++W+  MNS+I P Y+VSFK      + +   
Sbjct: 185 IVTGSKQSYPSSNQFDSGVDNLENPRKYVIWSCNMNSYILPTYIVSFK------SHLLRG 244

Query: 252 SVMKPTSPWMSFPTLMSALSRFLDPGRMSLIQKCHKDFVRNKIPRPQLINRVRHIAGDEL 311
            + +  SP +SF  LMS LS+ LD  RM+LI   + DF + K+ R QL+ ++R + GD L
Sbjct: 245 LIGRARSPCVSFSVLMSILSKSLDAARMNLILTSYDDFRKRKLRREQLVRKIREVVGDNL 304

Query: 312 LIALIKKHRR 321
           L  ++K  RR
Sbjct: 305 LFKILKNQRR 305

BLAST of Lag0005319 vs. TAIR 10
Match: AT5G62520.1 (similar to RCD one 5 )

HSP 1 Score: 194.5 bits (493), Expect = 1.3e-49
Identity = 118/315 (37.46%), Postives = 178/315 (56.51%), Query Frame = 0

Query: 6   IEERISITIDDYESLGSGNYEGDEYDSLTSNRFRFLQASGMTRLEDKGVEHQTVAKSFVS 65
           +  ++    DD E  GS   E    DS  S+   F    G+  L +    H  + ++  S
Sbjct: 4   VRTQVEAVFDDSEQDGSTISESGSCDS--SSDRSFADELGLMELLEGDKAHDLIYRNCKS 63

Query: 66  GLGLLGRDARVVAVHKNWSSDSGKTARAEAFRIFSDAVAAKCGGD--ANIKFAWYGAPID 125
           G   LG   ++++V +N   + G  A+ + F++F +AV  K GGD  A +K+ W      
Sbjct: 64  G---LGDQCQILSVLRNGFRNVGSRAKLKTFQVFQEAVQMKHGGDGGAKVKYGWCSVSKH 123

Query: 126 EICDIVSHGFSHFSHNNNDADSESSHGVGVHLSPGRFSIDCVSSSAAD-RNGLRHMLLCR 185
           E+  I  +GFS    N      + S G G++LSP    +DC+  SA++  +G+R +LLCR
Sbjct: 124 ELKTIFEYGFSEPLRN------DGSFGRGLYLSPDNSPLDCLKDSASESEDGMRFLLLCR 183

Query: 186 VILGKVEAIPVGSTQSHPTSSAFDTAVDSLFAPRKYIVWAAFMNSHIQPAYVVSFKAPDP 245
           V+LGK E +P GST+S P+S  FD+ VD L + +KYIVW+  MN+H+ P ++V  KAP  
Sbjct: 184 VLLGKSEIVPQGSTRSCPSSPEFDSGVDDLVSTKKYIVWSTHMNTHVLPEFLVCIKAP-- 243

Query: 246 NDAQMN-SVSVMKPTSPWMSFPTLMSALSRFLDPGRMSLIQKCHKDFVRNKIPRPQLINR 305
                N + S  +  SPWM+FP L+ ALS+FL P ++ +IQK +KD    +I R +LI R
Sbjct: 244 ----FNLTRSPKRLRSPWMAFPVLIKALSKFLPPSQILVIQKHYKDQQNRRITRSELIQR 301

Query: 306 VRHIAGDELLIALIK 317
           VR I GD+LL+ +IK
Sbjct: 304 VRSITGDKLLVHIIK 301

BLAST of Lag0005319 vs. TAIR 10
Match: AT3G47720.1 (similar to RCD one 4 )

HSP 1 Score: 144.1 bits (362), Expect = 2.1e-34
Identity = 97/305 (31.80%), Postives = 160/305 (52.46%), Query Frame = 0

Query: 28  DEYDSLTSNRFRFLQASGMTRLEDKGVEHQTVAKSFVSGL-GLLGRDARVVAVHKNWSSD 87
           D+  S  +N F      G+T L      ++ + +   S L   L     +V++ KN    
Sbjct: 34  DQQHSSIANEF------GLTELPKDDKVYELIYRHCQSKLTSHLSNQFEIVSILKNGFQT 93

Query: 88  SGKTARAEAFRIFSDAVAAKCGG----------DANIKFAWYGAPIDEICDIVSHGFSHF 147
               A+ +AF+I++++VA K G            A +K+   G   +E+  I+ +GFS  
Sbjct: 94  PLGQAKLKAFQIYAESVAKKSGSCCGNKAAVAEAARVKYGCCGVEKEELKAILMYGFS-- 153

Query: 148 SHNNNDADSESSHGVGVHLSPGRFSIDCV--SSSAADRNGLRHMLLCRVILGKVEAIPVG 207
              NN           + LSP    + C+   SS+ + +G+  +L  R+I+GK E +   
Sbjct: 154 ---NN----------ALCLSPDNAPLQCMIDPSSSCNEDGISFLLFSRIIMGKSEVV-CS 213

Query: 208 STQSHPTSSAFDTAVDSLFAPRKYIVWAAFMNSHIQPAYVVSFKAPDPNDAQMNSVSVMK 267
           ++QS+P+S  FD+ VDSL +P KYI+W+  MN+H+ P +VV  K P          S++K
Sbjct: 214 TSQSYPSSMEFDSGVDSLTSPNKYIIWSTHMNTHVLPEFVVCIKTP----------SILK 273

Query: 268 ---PTSPWMSFPTLMSALSRFLDPGRMSLIQKCHKDFVRNKIPRPQLINRVRHIAGDELL 317
              P SPW+SFP L++++S+FL+  ++ LI K +K+    +I R +LI R+R I GD LL
Sbjct: 274 RKNPKSPWISFPVLINSISKFLNQSQIRLIHKHYKEHQDRRISRCELIQRLRSITGDSLL 306

BLAST of Lag0005319 vs. TAIR 10
Match: AT5G62520.2 (similar to RCD one 5 )

HSP 1 Score: 141.7 bits (356), Expect = 1.0e-33
Identity = 84/238 (35.29%), Postives = 130/238 (54.62%), Query Frame = 0

Query: 6   IEERISITIDDYESLGSGNYEGDEYDSLTSNRFRFLQASGMTRLEDKGVEHQTVAKSFVS 65
           +  ++    DD E  GS   E    DS  S+   F    G+  L +    H  + ++  S
Sbjct: 4   VRTQVEAVFDDSEQDGSTISESGSCDS--SSDRSFADELGLMELLEGDKAHDLIYRNCKS 63

Query: 66  GLGLLGRDARVVAVHKNWSSDSGKTARAEAFRIFSDAVAAKCGGD--ANIKFAWYGAPID 125
           G   LG   ++++V +N   + G  A+ + F++F +AV  K GGD  A +K+ W      
Sbjct: 64  G---LGDQCQILSVLRNGFRNVGSRAKLKTFQVFQEAVQMKHGGDGGAKVKYGWCSVSKH 123

Query: 126 EICDIVSHGFSHFSHNNNDADSESSHGVGVHLSPGRFSIDCVSSSAAD-RNGLRHMLLCR 185
           E+  I  +GFS    N      + S G G++LSP    +DC+  SA++  +G+R +LLCR
Sbjct: 124 ELKTIFEYGFSEPLRN------DGSFGRGLYLSPDNSPLDCLKDSASESEDGMRFLLLCR 183

Query: 186 VILGKVEAIPVGSTQSHPTSSAFDTAVDSLFAPRKYIVWAAFMNSHIQPAYVVSFKAP 241
           V+LGK E +P GST+S P+S  FD+ VD L + +KYIVW+  MN+H+ P ++V  KAP
Sbjct: 184 VLLGKSEIVPQGSTRSCPSSPEFDSGVDDLVSTKKYIVWSTHMNTHVLPEFLVCIKAP 230

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
XP_022951201.11.9e-13575.99probable inactive poly [ADP-ribose] polymerase SRO2 [Cucurbita moschata][more]
XP_023538139.13.3e-13575.68probable inactive poly [ADP-ribose] polymerase SRO2 [Cucurbita pepo subsp. pepo][more]
KAG6585158.12.1e-13475.68putative inactive poly [ADP-ribose] polymerase SRO2, partial [Cucurbita argyrosp... [more]
XP_023002607.11.7e-13174.77probable inactive poly [ADP-ribose] polymerase SRO2 [Cucurbita maxima][more]
KAG7020078.19.7e-9576.03putative inactive poly [ADP-ribose] polymerase SRO2, partial [Cucurbita argyrosp... [more]
Match NameE-valueIdentityDescription
Q9ZUD98.3e-6543.79Probable inactive poly [ADP-ribose] polymerase SRO2 OS=Arabidopsis thaliana OX=3... [more]
O645921.1e-4836.13Probable inactive poly [ADP-ribose] polymerase SRO3 OS=Arabidopsis thaliana OX=3... [more]
Q9FJJ31.9e-4837.46Probable inactive poly [ADP-ribose] polymerase SRO5 OS=Arabidopsis thaliana OX=3... [more]
Q9STU12.9e-3331.80Probable inactive poly [ADP-ribose] polymerase SRO4 OS=Arabidopsis thaliana OX=3... [more]
O822895.5e-2428.73Probable inactive poly [ADP-ribose] polymerase SRO1 OS=Arabidopsis thaliana OX=3... [more]
Match NameE-valueIdentityDescription
A0A6J1GGW99.4e-13675.99probable inactive poly [ADP-ribose] polymerase SRO2 OS=Cucurbita moschata OX=366... [more]
A0A6J1KJZ78.2e-13274.77probable inactive poly [ADP-ribose] polymerase SRO2 OS=Cucurbita maxima OX=3661 ... [more]
A0A5B7AWT57.3e-8851.08Poly [ADP-ribose] polymerase OS=Davidia involucrata OX=16924 GN=Din_030016 PE=4 ... [more]
A0A5B7AX479.5e-8851.08Poly [ADP-ribose] polymerase OS=Davidia involucrata OX=16924 GN=Din_030019 PE=4 ... [more]
A0A5N5G7K71.6e-8753.52Inactive poly [ADP-ribose] polymerase SRO2 OS=Pyrus ussuriensis x Pyrus communis... [more]
Match NameE-valueIdentityDescription
AT1G23550.15.9e-6643.79similar to RCD one 2 [more]
AT1G70440.17.8e-5036.13similar to RCD one 3 [more]
AT5G62520.11.3e-4937.46similar to RCD one 5 [more]
AT3G47720.12.1e-3431.80similar to RCD one 4 [more]
AT5G62520.21.0e-3335.29similar to RCD one 5 [more]
InterPro
Analysis Name: InterPro Annotations of Sponge gourd (AG-4) v1
Date Performed: 2022-08-01
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR022003RST domainPFAMPF12174RSTcoord: 254..319
e-value: 9.6E-25
score: 86.3
IPR022003RST domainPROSITEPS51879RSTcoord: 253..324
score: 29.688393
NoneNo IPR availableGENE3D3.90.228.10coord: 44..239
e-value: 7.9E-29
score: 102.9
NoneNo IPR availablePANTHERPTHR32263:SF14INACTIVE POLY [ADP-RIBOSE] POLYMERASE SRO2-RELATEDcoord: 28..320
NoneNo IPR availableSUPERFAMILY56399ADP-ribosylationcoord: 46..255
IPR044964Inactive poly [ADP-ribose] polymerase RCD1/SRO1-5PANTHERPTHR32263INACTIVE POLY [ADP-RIBOSE] POLYMERASE SRO4-RELATEDcoord: 28..320
IPR012317Poly(ADP-ribose) polymerase, catalytic domainPROSITEPS51059PARP_CATALYTICcoord: 32..259
score: 25.532825

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Lag0005319.1Lag0005319.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
molecular_function GO:0003950 NAD+ ADP-ribosyltransferase activity