Lag0005144 (gene) Sponge gourd (AG‐4) v1

Overview
NameLag0005144
Typegene
OrganismLuffa acutangula (Sponge gourd (AG‐4) v1)
DescriptionCaM_binding domain-containing protein
Locationchr6: 11147101 .. 11148570 (-)
RNA-Seq ExpressionLag0005144
SyntenyLag0005144
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonCDSpolypeptide
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGGCAACAACTAAAGCAAAAGAAAGTTGTGGTTTGGGGAAGGAGAGGAAGATCACAGCTCCTAGTTCTATCACTGTTCCTAATCATACTTCAACAAAGAGAACTCCAAAATCATCTCCAAGAGCTCCCTCCACTGATCATCAGAAACTATCTTCTTTACCTTCCTCTTCCTCCTCTTCTGCTCCTTCACTTTCGACACACGCCGAGAAACATGTTCCGAACTATCTCAAGTCGACGGCCAGCTCCCGCCATGACCATAGCAGTAGCTTCAAGCCAATCAAGAAGACTACTACTACTACTGCAGCCCCTGCACCAATACCTGAGGGGAAGCCAAGTTTGAATAGAAGAAGATCTTTTGACAAACCGCCCCCGACCGCACCGCGTTTGGACAAGCCGTTTCGATCGCCTGGACCGAGGAATAGGGCGGCTCATGTTCCCGTCCGGTCTTCCTCTTTTGGGGCCAAACCCTCTTCCACCACAATTCTCAGTAGTTCTTCAAAGGTCTTGGAGAGATCAAGTTCCAAAAACTCCAAGGTGGGAGGAGGAGGGAAACCACTGCAACCTGTTCATACTATGATCAGAAGTAGTAGCAATGTGAAGAAAAGCTTGAGGAGAGATTCTAGCAATGCTGCTGCTGCTGCTGCTAAGAATGCTGTTGAACATGTCGACCGGAGTCCTTCTTTTGTTCTCGGCATGAACGAGGAGGAATTGAAAAAGATCGAGTACGAACTCGATCCGTTTCTTCCTGATCCAATGCCTGAATTAGATAAACAACTTACAACAACTGATCAGGTGGAAAAGAAACAAGTTCTGGATAAAGAGGAAGTTGAGGCTGAGAAGAAGCTTCACCATCAGCAGCAGCCTACAAATGACACAGAGATCTCCAAAGTAGTTGTTTCTAATAAAGAGGTAGCTGAGACTGATGCGAAATCCCCGGTCCAGGAAGACGAGAATCGAGAACGAAATCAAGAAGAAGAAGAAGAAGAAGCCAAAGAAGAGGAGAAACCAGAGAGAATTGTAGTAGAAAAAGAGGATGTTGTTGCTGTTGTTGTTACATCAGAGGAAAACACAAACAACAATAATGAGGAGAAGATGGTAACAGAAGAGAAGAGCGAGACCGAGACCGATCCCGAGGTCTCGGAAGGAGGCGAAGGAGCCGTTGTTTTAGATCAGAAAGAAGCACAAGATGGGCAGAGTTTCAAGGAAGAAAATCCCAACAGCAGCAGTCCAGCAAAGGCAGAGACACCAGAGAAAGAGACCGAAGCGACCGAGAAGATGGCGGTAGCAGCAGCGGAGAAGGCGGCAGGCAAACCACGACAAGGCGGACCGGGAAGGAAGGAGTCTCCAGCAATGTACAATCATGTAATAGAGGAGACAGCAAGCAAGCTTTTGGAAAAGAGGAAGAACAAGGTAAGAGCATTGGCTGGGGCATTTCAGACAGTCATTGACTATGAATCTTCATCCAAATGA

mRNA sequence

ATGGCAACAACTAAAGCAAAAGAAAGTTGTGGTTTGGGGAAGGAGAGGAAGATCACAGCTCCTAGTTCTATCACTGTTCCTAATCATACTTCAACAAAGAGAACTCCAAAATCATCTCCAAGAGCTCCCTCCACTGATCATCAGAAACTATCTTCTTTACCTTCCTCTTCCTCCTCTTCTGCTCCTTCACTTTCGACACACGCCGAGAAACATGTTCCGAACTATCTCAAGTCGACGGCCAGCTCCCGCCATGACCATAGCAGTAGCTTCAAGCCAATCAAGAAGACTACTACTACTACTGCAGCCCCTGCACCAATACCTGAGGGGAAGCCAAGTTTGAATAGAAGAAGATCTTTTGACAAACCGCCCCCGACCGCACCGCGTTTGGACAAGCCGTTTCGATCGCCTGGACCGAGGAATAGGGCGGCTCATGTTCCCGTCCGGTCTTCCTCTTTTGGGGCCAAACCCTCTTCCACCACAATTCTCAGTAGTTCTTCAAAGGTCTTGGAGAGATCAAGTTCCAAAAACTCCAAGGTGGGAGGAGGAGGGAAACCACTGCAACCTGTTCATACTATGATCAGAAGTAGTAGCAATGTGAAGAAAAGCTTGAGGAGAGATTCTAGCAATGCTGCTGCTGCTGCTGCTAAGAATGCTGTTGAACATGTCGACCGGAGTCCTTCTTTTGTTCTCGGCATGAACGAGGAGGAATTGAAAAAGATCGAGTACGAACTCGATCCGTTTCTTCCTGATCCAATGCCTGAATTAGATAAACAACTTACAACAACTGATCAGGTGGAAAAGAAACAAGTTCTGGATAAAGAGGAAGTTGAGGCTGAGAAGAAGCTTCACCATCAGCAGCAGCCTACAAATGACACAGAGATCTCCAAAGTAGTTGTTTCTAATAAAGAGGTAGCTGAGACTGATGCGAAATCCCCGGTCCAGGAAGACGAGAATCGAGAACGAAATCAAGAAGAAGAAGAAGAAGAAGCCAAAGAAGAGGAGAAACCAGAGAGAATTGTAGTAGAAAAAGAGGATGTTGTTGCTGTTGTTGTTACATCAGAGGAAAACACAAACAACAATAATGAGGAGAAGATGGTAACAGAAGAGAAGAGCGAGACCGAGACCGATCCCGAGGTCTCGGAAGGAGGCGAAGGAGCCGTTGTTTTAGATCAGAAAGAAGCACAAGATGGGCAGAGTTTCAAGGAAGAAAATCCCAACAGCAGCAGTCCAGCAAAGGCAGAGACACCAGAGAAAGAGACCGAAGCGACCGAGAAGATGGCGGTAGCAGCAGCGGAGAAGGCGGCAGGCAAACCACGACAAGGCGGACCGGGAAGGAAGGAGTCTCCAGCAATGTACAATCATGTAATAGAGGAGACAGCAAGCAAGCTTTTGGAAAAGAGGAAGAACAAGGTAAGAGCATTGGCTGGGGCATTTCAGACAGTCATTGACTATGAATCTTCATCCAAATGA

Coding sequence (CDS)

ATGGCAACAACTAAAGCAAAAGAAAGTTGTGGTTTGGGGAAGGAGAGGAAGATCACAGCTCCTAGTTCTATCACTGTTCCTAATCATACTTCAACAAAGAGAACTCCAAAATCATCTCCAAGAGCTCCCTCCACTGATCATCAGAAACTATCTTCTTTACCTTCCTCTTCCTCCTCTTCTGCTCCTTCACTTTCGACACACGCCGAGAAACATGTTCCGAACTATCTCAAGTCGACGGCCAGCTCCCGCCATGACCATAGCAGTAGCTTCAAGCCAATCAAGAAGACTACTACTACTACTGCAGCCCCTGCACCAATACCTGAGGGGAAGCCAAGTTTGAATAGAAGAAGATCTTTTGACAAACCGCCCCCGACCGCACCGCGTTTGGACAAGCCGTTTCGATCGCCTGGACCGAGGAATAGGGCGGCTCATGTTCCCGTCCGGTCTTCCTCTTTTGGGGCCAAACCCTCTTCCACCACAATTCTCAGTAGTTCTTCAAAGGTCTTGGAGAGATCAAGTTCCAAAAACTCCAAGGTGGGAGGAGGAGGGAAACCACTGCAACCTGTTCATACTATGATCAGAAGTAGTAGCAATGTGAAGAAAAGCTTGAGGAGAGATTCTAGCAATGCTGCTGCTGCTGCTGCTAAGAATGCTGTTGAACATGTCGACCGGAGTCCTTCTTTTGTTCTCGGCATGAACGAGGAGGAATTGAAAAAGATCGAGTACGAACTCGATCCGTTTCTTCCTGATCCAATGCCTGAATTAGATAAACAACTTACAACAACTGATCAGGTGGAAAAGAAACAAGTTCTGGATAAAGAGGAAGTTGAGGCTGAGAAGAAGCTTCACCATCAGCAGCAGCCTACAAATGACACAGAGATCTCCAAAGTAGTTGTTTCTAATAAAGAGGTAGCTGAGACTGATGCGAAATCCCCGGTCCAGGAAGACGAGAATCGAGAACGAAATCAAGAAGAAGAAGAAGAAGAAGCCAAAGAAGAGGAGAAACCAGAGAGAATTGTAGTAGAAAAAGAGGATGTTGTTGCTGTTGTTGTTACATCAGAGGAAAACACAAACAACAATAATGAGGAGAAGATGGTAACAGAAGAGAAGAGCGAGACCGAGACCGATCCCGAGGTCTCGGAAGGAGGCGAAGGAGCCGTTGTTTTAGATCAGAAAGAAGCACAAGATGGGCAGAGTTTCAAGGAAGAAAATCCCAACAGCAGCAGTCCAGCAAAGGCAGAGACACCAGAGAAAGAGACCGAAGCGACCGAGAAGATGGCGGTAGCAGCAGCGGAGAAGGCGGCAGGCAAACCACGACAAGGCGGACCGGGAAGGAAGGAGTCTCCAGCAATGTACAATCATGTAATAGAGGAGACAGCAAGCAAGCTTTTGGAAAAGAGGAAGAACAAGGTAAGAGCATTGGCTGGGGCATTTCAGACAGTCATTGACTATGAATCTTCATCCAAATGA

Protein sequence

MATTKAKESCGLGKERKITAPSSITVPNHTSTKRTPKSSPRAPSTDHQKLSSLPSSSSSSAPSLSTHAEKHVPNYLKSTASSRHDHSSSFKPIKKTTTTTAAPAPIPEGKPSLNRRRSFDKPPPTAPRLDKPFRSPGPRNRAAHVPVRSSSFGAKPSSTTILSSSSKVLERSSSKNSKVGGGGKPLQPVHTMIRSSSNVKKSLRRDSSNAAAAAAKNAVEHVDRSPSFVLGMNEEELKKIEYELDPFLPDPMPELDKQLTTTDQVEKKQVLDKEEVEAEKKLHHQQQPTNDTEISKVVVSNKEVAETDAKSPVQEDENRERNQEEEEEEAKEEEKPERIVVEKEDVVAVVVTSEENTNNNNEEKMVTEEKSETETDPEVSEGGEGAVVLDQKEAQDGQSFKEENPNSSSPAKAETPEKETEATEKMAVAAAEKAAGKPRQGGPGRKESPAMYNHVIEETASKLLEKRKNKVRALAGAFQTVIDYESSSK
Homology
BLAST of Lag0005144 vs. NCBI nr
Match: KAG6598809.1 (hypothetical protein SDJN03_08587, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 500.0 bits (1286), Expect = 2.4e-137
Identity = 338/511 (66.14%), Postives = 383/511 (74.95%), Query Frame = 0

Query: 1   MATTKAKESCGLGKERKITAPSSITVPN-HTSTKRTPKSSPRAPSTDHQKLSSLP----S 60
           MAT++AKESCGLGKERKITAPSS+T+PN +TSTKRTPKSSPR  STD QKLSS P    S
Sbjct: 1   MATSRAKESCGLGKERKITAPSSVTIPNLNTSTKRTPKSSPRGISTD-QKLSSFPSSPSS 60

Query: 61  SSSSSAPSLSTHAEKHVPNYLKSTASSRHDHSSSFKPIKKTTTTTAAPAPIPEGKPSLNR 120
           SSSSS  SLS   +KHVPNYLKSTASSRHDH  +FKPIKK    +  PAPIPEGKPSLNR
Sbjct: 61  SSSSSTHSLSILPQKHVPNYLKSTASSRHDH--NFKPIKK----SGGPAPIPEGKPSLNR 120

Query: 121 RRSFDKPPPTAPRLDKPFRSPGPRNRAAHVPVRSSSFGAKPSSTTILSSSSKVLERSSSK 180
           RRSFDKPPP   RL KPFRSP PRNRAAH PVRSSSFGAKPS++ + S+ +  LERS SK
Sbjct: 121 RRSFDKPPPATQRLGKPFRSPEPRNRAAHAPVRSSSFGAKPSTSIVSSTKTGFLERSGSK 180

Query: 181 NSKVGGGGKPLQPVHTMIRSSSNVKKSLRRDSSNAAAAAA-------KNAVEHVDRSPSF 240
             KV  GGK  Q V T ++SSSNV+KSL+R+SSNAA+A++       KNAVEHV ++P  
Sbjct: 181 IYKV--GGKTQQSVQT-LKSSSNVRKSLKRESSNAASASSAEMVPKTKNAVEHVGQTPVL 240

Query: 241 VLGMNEEELKKIEYELDPFLPDPMPELDKQLTTTDQVEKKQVLDKEEVEAEKKLHHQQQP 300
           VLG NEE+LKKIE ELD  LPDP PELDK+L+TTDQV             EK+LHH  QP
Sbjct: 241 VLGANEEDLKKIECELDLCLPDPRPELDKELSTTDQV-------------EKRLHH-HQP 300

Query: 301 TNDTEISKVVVSNKEVAET-------DAKSPVQEDENRERNQEEEEEEAKEEEKPERIVV 360
             DT+I KVV SNK+V ET       +AKS ++EDENRE+N+ E EEEAKEEEKPERI V
Sbjct: 301 IQDTKIFKVVGSNKKVEETPGAAETDNAKSSIREDENREQNEGEGEEEAKEEEKPERIEV 360

Query: 361 EKEDVVAVVVTSEENTNNNNEEKMVTEEKSETETDPEVSEGGEGAVVLDQKEAQDGQSFK 420
           EKE+V   VV SEE+TNN NEE+       ++ TDP      EG +VL+ KE +DGQS K
Sbjct: 361 EKEEVFG-VVASEESTNNKNEEE-------KSHTDPI----QEGVIVLEHKEVEDGQSSK 420

Query: 421 EENPNSSSPAKAETPEKETEATEKM---AVAAAEKAAGKPRQGGPGRKESPAMYNHVIEE 480
           EEN NS+        EKE EATEK+   A AAAE AA KPRQGGPGRKESPA+YNHVIEE
Sbjct: 421 EENHNST--------EKEAEATEKVAAAATAAAEAAAAKPRQGGPGRKESPAVYNHVIEE 467

Query: 481 TASKLLEKRKNKVRALAGAFQTVIDYESSSK 490
           TASKLLEKRKNKVRALAGAFQTVIDYESSSK
Sbjct: 481 TASKLLEKRKNKVRALAGAFQTVIDYESSSK 467

BLAST of Lag0005144 vs. NCBI nr
Match: XP_023546370.1 (cell wall protein RBR3-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 499.6 bits (1285), Expect = 3.1e-137
Identity = 339/512 (66.21%), Postives = 382/512 (74.61%), Query Frame = 0

Query: 1   MATTKAKESCGLGKERKITAPSSITVPN-HTSTKRTPKSSPRAPSTDHQKLSSLP----S 60
           MAT++AKESCGLGKERKITAPSS+T+PN +TSTKRTPKSSPR  STD QKLSS P    S
Sbjct: 1   MATSRAKESCGLGKERKITAPSSVTIPNVNTSTKRTPKSSPRGISTD-QKLSSFPSSPSS 60

Query: 61  SSSSSAPSLSTHAEKHVPNYLKSTASSRHDHSSSFKPIKKTTTTTAAPAPIPEGKPSLNR 120
           SSSSS  SLS   +KHVPNYLKSTASSRHDH  +FKPIKK    +A P PIPEGKPSLNR
Sbjct: 61  SSSSSTHSLSILPQKHVPNYLKSTASSRHDH--NFKPIKK----SAGPTPIPEGKPSLNR 120

Query: 121 RRSFDKPPPTAPRLDKPFRSPGPRNRAAHVPVRSSSFGAKPSSTTILSSSSKVLERSSSK 180
           RRSFDKPPP   RL KPFRSP PRNRAAH PVRSSSFGAKPS+T + S+ +  LERS SK
Sbjct: 121 RRSFDKPPPATQRLGKPFRSPEPRNRAAHAPVRSSSFGAKPSTTIVSSTKTGFLERSGSK 180

Query: 181 NSKVGGGGKPLQPVHTMIRSSSNVKKSLRRDSSNAAAAAA-------KNAVEHVDRSPSF 240
             KV  GGK  Q V T ++SSSNV+KSL+R+SSNAA+A++       KNAVEHV +SP  
Sbjct: 181 IYKV--GGKTQQSVQT-LKSSSNVRKSLKRESSNAASASSAEMVPKTKNAVEHVGQSPVL 240

Query: 241 VLGMNEEELKKIEYELDPFLPDPMPELDKQLTTTDQVEKKQVLDKEEVEAEKKLHHQQQP 300
           VLG NEE+LKKIE ELD  LPDP PELDK+L+TTDQV             EK+LHH  QP
Sbjct: 241 VLGANEEDLKKIECELDLCLPDPRPELDKELSTTDQV-------------EKRLHH-HQP 300

Query: 301 TNDTEISKVVVSNKEVAET-------DAKSPVQEDENRERNQEEEEEEAKEEEKPERIVV 360
             DT+I KVV SNK+V ET       +AKS ++EDENRE+N+ E EEEAKEEEKPERI V
Sbjct: 301 IQDTKIFKVVGSNKKVEETPGAAETDNAKSSIREDENREQNEGEGEEEAKEEEKPERIEV 360

Query: 361 EKEDVVAVVVTSEENTNNNNEEKMVTEEKSETETDPEVSEGGEGAVVLDQKEAQDGQSFK 420
           EKE+V   VV SEE+TNN NEE+       ++ TDP       G +VL+ KE +DGQS K
Sbjct: 361 EKEEVFG-VVASEESTNNKNEEE-------KSHTDPI----QVGVIVLEHKEVEDGQSSK 420

Query: 421 EENPNSSSPAKAETPEKETEATEKM----AVAAAEKAAGKPRQGGPGRKESPAMYNHVIE 480
           EEN NS+        EKE EATEK+    A AAAE AA KPRQGGPGRKESPA+YNHVIE
Sbjct: 421 EENHNST--------EKEAEATEKVAAAAAAAAAEAAAAKPRQGGPGRKESPAVYNHVIE 468

Query: 481 ETASKLLEKRKNKVRALAGAFQTVIDYESSSK 490
           ETASKLLEKRKNKVRALAGAFQTVIDYESSSK
Sbjct: 481 ETASKLLEKRKNKVRALAGAFQTVIDYESSSK 468

BLAST of Lag0005144 vs. NCBI nr
Match: KAG7029749.1 (hypothetical protein SDJN02_08091, partial [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 499.2 bits (1284), Expect = 4.0e-137
Identity = 338/511 (66.14%), Postives = 382/511 (74.76%), Query Frame = 0

Query: 1   MATTKAKESCGLGKERKITAPSSITVPN-HTSTKRTPKSSPRAPSTDHQKLSSLP----S 60
           MAT++AKESCGLGKERKITAPSS+T+PN +TSTKRTPKSSPR  STD QKLSS P    S
Sbjct: 1   MATSRAKESCGLGKERKITAPSSVTIPNLNTSTKRTPKSSPRGISTD-QKLSSFPSSPSS 60

Query: 61  SSSSSAPSLSTHAEKHVPNYLKSTASSRHDHSSSFKPIKKTTTTTAAPAPIPEGKPSLNR 120
           SSSSS  SLS   +KHVPNYLKSTASSRHDH  +FKPIKK    +  PAPIPEGKPSLNR
Sbjct: 61  SSSSSTHSLSILPQKHVPNYLKSTASSRHDH--NFKPIKK----SGGPAPIPEGKPSLNR 120

Query: 121 RRSFDKPPPTAPRLDKPFRSPGPRNRAAHVPVRSSSFGAKPSSTTILSSSSKVLERSSSK 180
           RRSFDKPPP   RL KPFRSP PRNRAAH PVRSSSFGAKPS++ + S+ +  LERS SK
Sbjct: 121 RRSFDKPPPATQRLGKPFRSPEPRNRAAHAPVRSSSFGAKPSTSIVSSTKTGFLERSGSK 180

Query: 181 NSKVGGGGKPLQPVHTMIRSSSNVKKSLRRDSSNAAAAAA-------KNAVEHVDRSPSF 240
             KV  GGK  Q V T ++SSSNV+KSL+R+SSNAA+A++       KNAVEHV ++P  
Sbjct: 181 IYKV--GGKTQQSVQT-LKSSSNVRKSLKRESSNAASASSAEMVPKTKNAVEHVGQTPVL 240

Query: 241 VLGMNEEELKKIEYELDPFLPDPMPELDKQLTTTDQVEKKQVLDKEEVEAEKKLHHQQQP 300
           VLG NEE+LKKIE ELD  LPDP PELDK+L+TTDQV             EK+LHH  QP
Sbjct: 241 VLGANEEDLKKIECELDLCLPDPRPELDKELSTTDQV-------------EKRLHH-HQP 300

Query: 301 TNDTEISKVVVSNKEVAET-------DAKSPVQEDENRERNQEEEEEEAKEEEKPERIVV 360
             DT+I KVV SNK+V ET       +AKS +QEDENRE+N+ E EEEAKEEEKPERI V
Sbjct: 301 IQDTKIFKVVGSNKKVEETPGAAETDNAKSSIQEDENREQNEGEGEEEAKEEEKPERIEV 360

Query: 361 EKEDVVAVVVTSEENTNNNNEEKMVTEEKSETETDPEVSEGGEGAVVLDQKEAQDGQSFK 420
           EKE+V   VV SEE+ NN NEE+       ++ TDP      EG +VL+ KE +DGQS K
Sbjct: 361 EKEEVFG-VVASEESRNNKNEEE-------KSHTDPI----QEGVIVLEHKEVEDGQSSK 420

Query: 421 EENPNSSSPAKAETPEKETEATEKMAVAA---AEKAAGKPRQGGPGRKESPAMYNHVIEE 480
           EEN NS+        EKE EATEK+A AA   AE AA KPRQGGPGRKESPA+YNHVIEE
Sbjct: 421 EENHNST--------EKEAEATEKVAAAATATAEAAAAKPRQGGPGRKESPAVYNHVIEE 467

Query: 481 TASKLLEKRKNKVRALAGAFQTVIDYESSSK 490
           TASKLLEKRKNKVRALAGAFQTVIDYESSSK
Sbjct: 481 TASKLLEKRKNKVRALAGAFQTVIDYESSSK 467

BLAST of Lag0005144 vs. NCBI nr
Match: XP_022997522.1 (microtubule-associated protein RP/EB family member 1-like [Cucurbita maxima])

HSP 1 Score: 498.8 bits (1283), Expect = 5.3e-137
Identity = 338/508 (66.54%), Postives = 382/508 (75.20%), Query Frame = 0

Query: 1   MATTKAKESCGLGKERKITAPSSITVPN-HTSTKRTPKSSPRAPSTDHQKLSSLPSS--- 60
           MAT++AKESCGLGKERKIT PSS+T+PN +TSTKRTPKSSPR  STD QKLSS PSS   
Sbjct: 1   MATSRAKESCGLGKERKITTPSSVTIPNPNTSTKRTPKSSPRGISTD-QKLSSFPSSPSP 60

Query: 61  SSSSAPSLSTHAEKHVPNYLKSTASSRHDHSSSFKPIKKTTTTTAAPAPIPEGKPSLNRR 120
           SSSS  SLS   +KHVPNYLKSTASSRHDH  +FKPIKK    +A PAPIPEGKPSLNRR
Sbjct: 61  SSSSTHSLSILPQKHVPNYLKSTASSRHDH--NFKPIKK----SAGPAPIPEGKPSLNRR 120

Query: 121 RSFDKPPPTAPRLDKPFRSPGPRNRAAHVPVRSSSFGAKPSSTTILSSSSKVLERSSSKN 180
           RSFDKPPP   RL KPFRSP PRNRAAH PVRSSSFGAKPS+T + S+ +  LERS SK 
Sbjct: 121 RSFDKPPPAMQRLGKPFRSPEPRNRAAHAPVRSSSFGAKPSTTLVSSTKTGFLERSGSKI 180

Query: 181 SKVGGGGKPLQPVHTMIRSSSNVKKSLRRDSSNAAAAAA-------KNAVEHVDRSPSFV 240
            KV  GGK  Q V T  +SSSN++KSL+R+SSNAA+A++       KNAVEHV +SP  V
Sbjct: 181 YKV--GGKTQQSVQT-FKSSSNLRKSLKRESSNAASASSAEMVPKTKNAVEHVGQSPVLV 240

Query: 241 LGMNEEELKKIEYELDPFLPDPMPELDKQLTTTDQVEKKQVLDKEEVEAEKKLHHQQQPT 300
           LG+NEE+LKKIE ELD  LPDP PELDK+L+TTDQV             EK+LHH  QP 
Sbjct: 241 LGVNEEDLKKIECELDLCLPDPRPELDKELSTTDQV-------------EKRLHH-HQPI 300

Query: 301 NDTEISKVVVSNKEVAET-------DAKSPVQEDENRERNQEEEEEEAKEEEKPERIVVE 360
            DT+I KVV SNK+V ET       +AKS + EDEN+E+N+ E EEEAKEEEKPERI VE
Sbjct: 301 QDTKIFKVVGSNKKVEETPGAAETDNAKSSIWEDENQEQNEGEGEEEAKEEEKPERIEVE 360

Query: 361 KEDVVAVVVTSEENTNNNNEEKMVTEEKSETETDPEVSEGGEGAVVLDQKEAQDGQSFKE 420
           KE+V   VV SEE+TNN NEE+       ++ TDP      EG +VL+ KEA+DGQS KE
Sbjct: 361 KEEVYG-VVASEESTNNKNEEE-------KSHTDPI----QEGVIVLEHKEAEDGQSSKE 420

Query: 421 ENPNSSSPAKAETPEKETEATEKMAVAAAEKA-AGKPRQGGPGRKESPAMYNHVIEETAS 480
           EN NS+        EKE EATEK+A AAA  A A KPRQGGPGRKESPA+YNHVIEETAS
Sbjct: 421 ENHNST--------EKEAEATEKVAAAAAAAAEAAKPRQGGPGRKESPAVYNHVIEETAS 464

Query: 481 KLLEKRKNKVRALAGAFQTVIDYESSSK 490
           KLLEKRKNKVRALAGAFQTVIDYESSSK
Sbjct: 481 KLLEKRKNKVRALAGAFQTVIDYESSSK 464

BLAST of Lag0005144 vs. NCBI nr
Match: XP_022962060.1 (neurofilament medium polypeptide-like [Cucurbita moschata])

HSP 1 Score: 498.0 bits (1281), Expect = 9.0e-137
Identity = 339/511 (66.34%), Postives = 381/511 (74.56%), Query Frame = 0

Query: 1   MATTKAKESCGLGKERKITAPSSITVPN-HTSTKRTPKSSPRAPSTDHQKLSSLP----S 60
           MAT++AKESCGLGKERKITAPSS+T+PN +TSTKRTPKSSPR  STD QKLSS P    S
Sbjct: 1   MATSRAKESCGLGKERKITAPSSVTIPNLNTSTKRTPKSSPRGISTD-QKLSSFPSSPSS 60

Query: 61  SSSSSAPSLSTHAEKHVPNYLKSTASSRHDHSSSFKPIKKTTTTTAAPAPIPEGKPSLNR 120
           SSSSS  SLS   +KHVPNYLKSTASSRHDH  +FKPIKK    +  PAPIPEGKPSLNR
Sbjct: 61  SSSSSTHSLSILPQKHVPNYLKSTASSRHDH--NFKPIKK----SGGPAPIPEGKPSLNR 120

Query: 121 RRSFDKPPPTAPRLDKPFRSPGPRNRAAHVPVRSSSFGAKPSSTTILSSSSKVLERSSSK 180
           RRSFDKPPP   RL KPFRSP PRNRAAH PVRSSSFGAKPS++ + S+ +  LERS SK
Sbjct: 121 RRSFDKPPPATQRLGKPFRSPEPRNRAAHAPVRSSSFGAKPSTSIVSSTKTGFLERSGSK 180

Query: 181 NSKVGGGGKPLQPVHTMIRSSSNVKKSLRRDSSNAAAAAA-------KNAVEHVDRSPSF 240
             KV  GGK  Q V T ++SSSNV+KSL+R+SSNAA+A++       KNAVEHV +SP  
Sbjct: 181 IYKV--GGKTQQSVQT-LKSSSNVRKSLKRESSNAASASSAEMVPKTKNAVEHVGQSPVL 240

Query: 241 VLGMNEEELKKIEYELDPFLPDPMPELDKQLTTTDQVEKKQVLDKEEVEAEKKLHHQQQP 300
           VLG NEE+LKKIE ELD  LPDP PELDK+L+TTDQV             EK+LHH  QP
Sbjct: 241 VLGANEEDLKKIECELDLCLPDPRPELDKELSTTDQV-------------EKRLHH-HQP 300

Query: 301 TNDTEISKVVVSNKEVAET-------DAKSPVQEDENRERNQEEEEEEAKEEEKPERIVV 360
             +T+I KVV SNK+V ET       +AKS ++EDENRE+N+ E EEEAKEEEKPERI V
Sbjct: 301 IQETKIFKVVGSNKKVEETPGAAETDNAKSSIREDENREQNEGEGEEEAKEEEKPERIEV 360

Query: 361 EKEDVVAVVVTSEENTNNNNEEKMVTEEKSETETDPEVSEGGEGAVVLDQKEAQDGQSFK 420
           EKE+V   VV SEE+TNN NE     EEKS T          EG +VL+ KEA+DGQS K
Sbjct: 361 EKEEVFG-VVASEESTNNKNE-----EEKSHTVPIQ------EGVIVLEHKEAEDGQSSK 420

Query: 421 EENPNSSSPAKAETPEKETEATEKMAVA---AAEKAAGKPRQGGPGRKESPAMYNHVIEE 480
           EEN  S+        EKETEATEK+A A   AAE  A KPRQGGPGRKESPA+YNHVIEE
Sbjct: 421 EENHTST--------EKETEATEKVAAAATSAAEAVAAKPRQGGPGRKESPAVYNHVIEE 467

Query: 481 TASKLLEKRKNKVRALAGAFQTVIDYESSSK 490
           TASKLLEKRKNKVRALAGAFQTVIDYESSSK
Sbjct: 481 TASKLLEKRKNKVRALAGAFQTVIDYESSSK 467

BLAST of Lag0005144 vs. ExPASy TrEMBL
Match: A0A6J1K599 (microtubule-associated protein RP/EB family member 1-like OS=Cucurbita maxima OX=3661 GN=LOC111492415 PE=4 SV=1)

HSP 1 Score: 498.8 bits (1283), Expect = 2.5e-137
Identity = 338/508 (66.54%), Postives = 382/508 (75.20%), Query Frame = 0

Query: 1   MATTKAKESCGLGKERKITAPSSITVPN-HTSTKRTPKSSPRAPSTDHQKLSSLPSS--- 60
           MAT++AKESCGLGKERKIT PSS+T+PN +TSTKRTPKSSPR  STD QKLSS PSS   
Sbjct: 1   MATSRAKESCGLGKERKITTPSSVTIPNPNTSTKRTPKSSPRGISTD-QKLSSFPSSPSP 60

Query: 61  SSSSAPSLSTHAEKHVPNYLKSTASSRHDHSSSFKPIKKTTTTTAAPAPIPEGKPSLNRR 120
           SSSS  SLS   +KHVPNYLKSTASSRHDH  +FKPIKK    +A PAPIPEGKPSLNRR
Sbjct: 61  SSSSTHSLSILPQKHVPNYLKSTASSRHDH--NFKPIKK----SAGPAPIPEGKPSLNRR 120

Query: 121 RSFDKPPPTAPRLDKPFRSPGPRNRAAHVPVRSSSFGAKPSSTTILSSSSKVLERSSSKN 180
           RSFDKPPP   RL KPFRSP PRNRAAH PVRSSSFGAKPS+T + S+ +  LERS SK 
Sbjct: 121 RSFDKPPPAMQRLGKPFRSPEPRNRAAHAPVRSSSFGAKPSTTLVSSTKTGFLERSGSKI 180

Query: 181 SKVGGGGKPLQPVHTMIRSSSNVKKSLRRDSSNAAAAAA-------KNAVEHVDRSPSFV 240
            KV  GGK  Q V T  +SSSN++KSL+R+SSNAA+A++       KNAVEHV +SP  V
Sbjct: 181 YKV--GGKTQQSVQT-FKSSSNLRKSLKRESSNAASASSAEMVPKTKNAVEHVGQSPVLV 240

Query: 241 LGMNEEELKKIEYELDPFLPDPMPELDKQLTTTDQVEKKQVLDKEEVEAEKKLHHQQQPT 300
           LG+NEE+LKKIE ELD  LPDP PELDK+L+TTDQV             EK+LHH  QP 
Sbjct: 241 LGVNEEDLKKIECELDLCLPDPRPELDKELSTTDQV-------------EKRLHH-HQPI 300

Query: 301 NDTEISKVVVSNKEVAET-------DAKSPVQEDENRERNQEEEEEEAKEEEKPERIVVE 360
            DT+I KVV SNK+V ET       +AKS + EDEN+E+N+ E EEEAKEEEKPERI VE
Sbjct: 301 QDTKIFKVVGSNKKVEETPGAAETDNAKSSIWEDENQEQNEGEGEEEAKEEEKPERIEVE 360

Query: 361 KEDVVAVVVTSEENTNNNNEEKMVTEEKSETETDPEVSEGGEGAVVLDQKEAQDGQSFKE 420
           KE+V   VV SEE+TNN NEE+       ++ TDP      EG +VL+ KEA+DGQS KE
Sbjct: 361 KEEVYG-VVASEESTNNKNEEE-------KSHTDPI----QEGVIVLEHKEAEDGQSSKE 420

Query: 421 ENPNSSSPAKAETPEKETEATEKMAVAAAEKA-AGKPRQGGPGRKESPAMYNHVIEETAS 480
           EN NS+        EKE EATEK+A AAA  A A KPRQGGPGRKESPA+YNHVIEETAS
Sbjct: 421 ENHNST--------EKEAEATEKVAAAAAAAAEAAKPRQGGPGRKESPAVYNHVIEETAS 464

Query: 481 KLLEKRKNKVRALAGAFQTVIDYESSSK 490
           KLLEKRKNKVRALAGAFQTVIDYESSSK
Sbjct: 481 KLLEKRKNKVRALAGAFQTVIDYESSSK 464

BLAST of Lag0005144 vs. ExPASy TrEMBL
Match: A0A6J1HE16 (neurofilament medium polypeptide-like OS=Cucurbita moschata OX=3662 GN=LOC111462632 PE=4 SV=1)

HSP 1 Score: 498.0 bits (1281), Expect = 4.3e-137
Identity = 339/511 (66.34%), Postives = 381/511 (74.56%), Query Frame = 0

Query: 1   MATTKAKESCGLGKERKITAPSSITVPN-HTSTKRTPKSSPRAPSTDHQKLSSLP----S 60
           MAT++AKESCGLGKERKITAPSS+T+PN +TSTKRTPKSSPR  STD QKLSS P    S
Sbjct: 1   MATSRAKESCGLGKERKITAPSSVTIPNLNTSTKRTPKSSPRGISTD-QKLSSFPSSPSS 60

Query: 61  SSSSSAPSLSTHAEKHVPNYLKSTASSRHDHSSSFKPIKKTTTTTAAPAPIPEGKPSLNR 120
           SSSSS  SLS   +KHVPNYLKSTASSRHDH  +FKPIKK    +  PAPIPEGKPSLNR
Sbjct: 61  SSSSSTHSLSILPQKHVPNYLKSTASSRHDH--NFKPIKK----SGGPAPIPEGKPSLNR 120

Query: 121 RRSFDKPPPTAPRLDKPFRSPGPRNRAAHVPVRSSSFGAKPSSTTILSSSSKVLERSSSK 180
           RRSFDKPPP   RL KPFRSP PRNRAAH PVRSSSFGAKPS++ + S+ +  LERS SK
Sbjct: 121 RRSFDKPPPATQRLGKPFRSPEPRNRAAHAPVRSSSFGAKPSTSIVSSTKTGFLERSGSK 180

Query: 181 NSKVGGGGKPLQPVHTMIRSSSNVKKSLRRDSSNAAAAAA-------KNAVEHVDRSPSF 240
             KV  GGK  Q V T ++SSSNV+KSL+R+SSNAA+A++       KNAVEHV +SP  
Sbjct: 181 IYKV--GGKTQQSVQT-LKSSSNVRKSLKRESSNAASASSAEMVPKTKNAVEHVGQSPVL 240

Query: 241 VLGMNEEELKKIEYELDPFLPDPMPELDKQLTTTDQVEKKQVLDKEEVEAEKKLHHQQQP 300
           VLG NEE+LKKIE ELD  LPDP PELDK+L+TTDQV             EK+LHH  QP
Sbjct: 241 VLGANEEDLKKIECELDLCLPDPRPELDKELSTTDQV-------------EKRLHH-HQP 300

Query: 301 TNDTEISKVVVSNKEVAET-------DAKSPVQEDENRERNQEEEEEEAKEEEKPERIVV 360
             +T+I KVV SNK+V ET       +AKS ++EDENRE+N+ E EEEAKEEEKPERI V
Sbjct: 301 IQETKIFKVVGSNKKVEETPGAAETDNAKSSIREDENREQNEGEGEEEAKEEEKPERIEV 360

Query: 361 EKEDVVAVVVTSEENTNNNNEEKMVTEEKSETETDPEVSEGGEGAVVLDQKEAQDGQSFK 420
           EKE+V   VV SEE+TNN NE     EEKS T          EG +VL+ KEA+DGQS K
Sbjct: 361 EKEEVFG-VVASEESTNNKNE-----EEKSHTVPIQ------EGVIVLEHKEAEDGQSSK 420

Query: 421 EENPNSSSPAKAETPEKETEATEKMAVA---AAEKAAGKPRQGGPGRKESPAMYNHVIEE 480
           EEN  S+        EKETEATEK+A A   AAE  A KPRQGGPGRKESPA+YNHVIEE
Sbjct: 421 EENHTST--------EKETEATEKVAAAATSAAEAVAAKPRQGGPGRKESPAVYNHVIEE 467

Query: 481 TASKLLEKRKNKVRALAGAFQTVIDYESSSK 490
           TASKLLEKRKNKVRALAGAFQTVIDYESSSK
Sbjct: 481 TASKLLEKRKNKVRALAGAFQTVIDYESSSK 467

BLAST of Lag0005144 vs. ExPASy TrEMBL
Match: A0A0A0LS85 (CaM_binding domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_2G378000 PE=4 SV=1)

HSP 1 Score: 459.9 bits (1182), Expect = 1.3e-125
Identity = 318/520 (61.15%), Postives = 363/520 (69.81%), Query Frame = 0

Query: 1   MATTKAKESCGLGKERKITAPSSITVPNHTSTKRTPKSSPRA--PSTDHQ----KLSSLP 60
           MATT++KESCGLGKERKIT PSS+T+P++TSTKRTPKSSPR    STD +      SS P
Sbjct: 1   MATTRSKESCGLGKERKITPPSSVTIPSNTSTKRTPKSSPRGIISSTDPKFPSFSPSSAP 60

Query: 61  SSSSSSAPSLSTHAEKHVPNYLKSTASSRHDHSSSFKPIKKTTTTTAAPAPIPEGKPSLN 120
           SS SS+  SLS   EKHVPNYLKSTASSR+DH  +FKPI +   + + PAPIPEG+PSLN
Sbjct: 61  SSPSSAPSSLSILPEKHVPNYLKSTASSRNDH--NFKPIAR---SKSGPAPIPEGRPSLN 120

Query: 121 RRRSFDKPPPTAPRLDKPFRSPGPRNRAAHVPVRSSSFGAKPSSTTIL---SSSSKVLER 180
           RRRSFDKPPPT PRL KPFRSPGPRNR  HVPVRSSSFGAKPS+TT     S+    LER
Sbjct: 121 RRRSFDKPPPTVPRLGKPFRSPGPRNRTPHVPVRSSSFGAKPSTTTTTIGHSTKPGFLER 180

Query: 181 SSSKNSKVGGGGKPLQPVHTMIRSSSNVKKSLRRDSSNAAAAA----------------- 240
           SSSK+SKV  GGKP  P+ ++  SSS VKKSLRR+SSNAAA+A                 
Sbjct: 181 SSSKSSKV--GGKPQPPIQSLKTSSSYVKKSLRRESSNAAASASASASASASASASAEIV 240

Query: 241 --AKNAVEHVDRSPSFVLGMNEEELKKIEYELDPFLPDPMPELDKQLTTTDQVEKKQVLD 300
             +KNAVEHVD+SPSFVLG+NEE+LKKIE ELDP+LPDPMPELDKQ+   DQVEKK VLD
Sbjct: 241 PKSKNAVEHVDQSPSFVLGVNEEDLKKIECELDPYLPDPMPELDKQI-RLDQVEKKAVLD 300

Query: 301 KEEVEAEKKLHHQQQPTNDTEISKVVVSNKEVAETDAKSPVQEDENRERNQEEEE---EE 360
           K+E               DTEI KVVV+NKEV E        E E    NQE+ E   EE
Sbjct: 301 KDE--------------TDTEILKVVVTNKEVEEQKETPRTVETEISLANQEQNEREGEE 360

Query: 361 AKEEEKPERIVVEKEDVVAVVVTSEENTNNNNEEKMVTEEKSETETDPEVSEGGEGAVVL 420
            KEE+ P+RI VEKE+VVA+    EE+ NN N+     +EKSET++              
Sbjct: 361 PKEEDNPKRIEVEKEEVVAL----EESINNKND-----QEKSETDS-------------- 420

Query: 421 DQKEAQDGQSFKEENPNSSSPAKAETPEKETEATEKMAVAAAEKAAGKPRQGGPGRKESP 480
                 DG+S KEE+  +S+P  AET EKE E+TEK A         KPRQGGPGRKESP
Sbjct: 421 ----VLDGESCKEESDQNSTPKAAETTEKEAESTEKTA---------KPRQGGPGRKESP 462

Query: 481 AMYNHVIEETASKLLEKRKNKVRALAGAFQTVIDYESSSK 490
           AMYNHVIEETASKLLEKRKNKVRALAGAFQTVIDYESSSK
Sbjct: 481 AMYNHVIEETASKLLEKRKNKVRALAGAFQTVIDYESSSK 462

BLAST of Lag0005144 vs. ExPASy TrEMBL
Match: A0A1S3BBU4 (serine/arginine repetitive matrix protein 1 OS=Cucumis melo OX=3656 GN=LOC103488229 PE=4 SV=1)

HSP 1 Score: 458.4 bits (1178), Expect = 3.8e-125
Identity = 318/510 (62.35%), Postives = 366/510 (71.76%), Query Frame = 0

Query: 1   MATTKAKESCGLGKERKITAPSSITVPNHTSTKRTPKSSPRA--PSTDHQ--KLSSLPSS 60
           MATT++KES GLGKER+IT PSS+T+PN+T TKRTPKSSPR    STD +    SS   S
Sbjct: 1   MATTRSKESGGLGKERRITPPSSVTIPNNTPTKRTPKSSPRGFISSTDPKVPSFSSSSPS 60

Query: 61  SSSSAPSLSTHAEKHVPNYLKSTASSRHDHSSSFKPIKKTTTTTAAPAPIPEGKPSLNRR 120
            SS+  SLS   EK VPNYLKSTASSR+DH  +FKPI   T + + PAPIPEGKPSLNRR
Sbjct: 61  PSSAPTSLSILPEKPVPNYLKSTASSRNDH--NFKPI---TRSKSGPAPIPEGKPSLNRR 120

Query: 121 RSFDKPPPTAPRLDKPFRSPGPRNRAAHVPVRSSSFGAKPS-STTILSSSSK--VLERSS 180
           RSFDKPPPTAPRL+KPFRSPGPRNRA HVPVRSSSFGAKPS STTI+  S+K   LERSS
Sbjct: 121 RSFDKPPPTAPRLEKPFRSPGPRNRATHVPVRSSSFGAKPSTSTTIIGHSTKPGFLERSS 180

Query: 181 SKNSKVGGGGKPLQPVHTMIRSSSNVKKSLRRDSSNAAAAAA-----------KNAVEHV 240
           SK SKV  GGKP  P+ ++  SSS+VKKSLRR+SSN AA+A+           KNAVEHV
Sbjct: 181 SKISKV--GGKPQPPIQSLKTSSSHVKKSLRRESSNVAASASASASAEIVPKTKNAVEHV 240

Query: 241 DRSPSFVLGMNEEELKKIEYELDPFLPDPMPELDKQLTTTDQVEKKQVLDKEEVEAEKKL 300
           D+SPSFVLG+NEE+LKKIE ELDP+LPDPMPELDKQ+   DQVEKK  LDK+E       
Sbjct: 241 DQSPSFVLGVNEEDLKKIECELDPYLPDPMPELDKQV-RIDQVEKKAALDKDE------- 300

Query: 301 HHQQQPTNDTEISKVVVSNKEVAETDAKSPVQED---ENRERNQEEEEEEAKEEEKPERI 360
                   DTEI KVVV+NKEV E +     + +    NRE+N E EEEE KEE+KP+RI
Sbjct: 301 -------TDTEILKVVVTNKEVEEKETPGTAETEISLANREQN-EGEEEEPKEEDKPKRI 360

Query: 361 VVEKEDVVAVVVTSEENTNNNNEEKMVTEEKSETETDPEVSEGGEGAVVLDQKEAQDGQS 420
            VEKE+V+A+    EE+ NN N+     +EKSET++                    DG+S
Sbjct: 361 GVEKEEVLAL----EESINNKND-----QEKSETDS------------------VLDGES 420

Query: 421 FKEENPNSSSPAKAETPEKETEATEKMAVAAAEKAAGKPRQGGPGRKESPAMYNHVIEET 480
            KEEN  +S+P  AET EKE E+TEK A         KPRQG PGRKESPA+YNHVIEET
Sbjct: 421 CKEENDQNSTPKAAETTEKEAESTEKTA---------KPRQGAPGRKESPAVYNHVIEET 451

Query: 481 ASKLLEKRKNKVRALAGAFQTVIDYESSSK 490
           ASKLLEKRKNKVRALAGAFQTVIDYESSSK
Sbjct: 481 ASKLLEKRKNKVRALAGAFQTVIDYESSSK 451

BLAST of Lag0005144 vs. ExPASy TrEMBL
Match: A0A5A7VBK4 (Serine/arginine repetitive matrix protein 1 OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold82G003220 PE=4 SV=1)

HSP 1 Score: 457.6 bits (1176), Expect = 6.5e-125
Identity = 318/512 (62.11%), Postives = 366/512 (71.48%), Query Frame = 0

Query: 1   MATTKAKESCGLGKERKITAPSSITVPNHTSTKRTPKSSPRA--PSTDHQ--KLSSLPSS 60
           MATT++KES GLGKER+IT PSS+T+PN+T TKRTPKSSPR    STD +    SS   S
Sbjct: 1   MATTRSKESGGLGKERRITPPSSVTIPNNTPTKRTPKSSPRGFISSTDPKVPSFSSSSPS 60

Query: 61  SSSSAPSLSTHAEKHVPNYLKSTASSRHDHSSSFKPIKKTTTTTAAPAPIPEGKPSLNRR 120
            SS+  SLS   EK VPNYLKSTASSR+DH  +FKPI   T + + PAPIPEGKPSLNRR
Sbjct: 61  PSSAPTSLSILPEKPVPNYLKSTASSRNDH--NFKPI---TRSKSGPAPIPEGKPSLNRR 120

Query: 121 RSFDKPPPTAPRLDKPFRSPGPRNRAAHVPVRSSSFGAKPS-STTILSSSSK--VLERSS 180
           RSFDKPPPTAPRL+KPFRSPGPRNRA HVPVRSSSFGAKPS STTI+  S+K   LERSS
Sbjct: 121 RSFDKPPPTAPRLEKPFRSPGPRNRATHVPVRSSSFGAKPSTSTTIIGHSTKPGFLERSS 180

Query: 181 SKNSKVGGGGKPLQPVHTMIRSSSNVKKSLRRDSSNAAAAAA-------------KNAVE 240
           SK SKV  GGKP  P+ ++  SSS+VKKSLRR+SSN AA+A+             KNAVE
Sbjct: 181 SKISKV--GGKPQPPIQSLKTSSSHVKKSLRRESSNVAASASASASASAEIVPKTKNAVE 240

Query: 241 HVDRSPSFVLGMNEEELKKIEYELDPFLPDPMPELDKQLTTTDQVEKKQVLDKEEVEAEK 300
           HVD+SPSFVLG+NEE+LKKIE ELDP+LPDPMPELDKQ+   DQVEKK  LDK+E     
Sbjct: 241 HVDQSPSFVLGVNEEDLKKIECELDPYLPDPMPELDKQV-RIDQVEKKAALDKDE----- 300

Query: 301 KLHHQQQPTNDTEISKVVVSNKEVAETDAKSPVQED---ENRERNQEEEEEEAKEEEKPE 360
                     DTEI KVVV+NKEV E +     + +    NRE+N E EEEE KEE+KP+
Sbjct: 301 ---------TDTEILKVVVTNKEVEEKETPGTAETEISLANREQN-EGEEEEPKEEDKPK 360

Query: 361 RIVVEKEDVVAVVVTSEENTNNNNEEKMVTEEKSETETDPEVSEGGEGAVVLDQKEAQDG 420
           RI VEKE+V+A+    EE+ NN N+     +EKSET++                    DG
Sbjct: 361 RIGVEKEEVLAL----EESINNKND-----QEKSETDS------------------VLDG 420

Query: 421 QSFKEENPNSSSPAKAETPEKETEATEKMAVAAAEKAAGKPRQGGPGRKESPAMYNHVIE 480
           +S KEEN  +S+P  AET EKE E+TEK A         KPRQG PGRKESPA+YNHVIE
Sbjct: 421 ESCKEENDQNSTPKAAETTEKEAESTEKTA---------KPRQGAPGRKESPAVYNHVIE 453

Query: 481 ETASKLLEKRKNKVRALAGAFQTVIDYESSSK 490
           ETASKLLEKRKNKVRALAGAFQTVIDYESSSK
Sbjct: 481 ETASKLLEKRKNKVRALAGAFQTVIDYESSSK 453

BLAST of Lag0005144 vs. TAIR 10
Match: AT5G24880.1 (BEST Arabidopsis thaliana protein match is: calmodulin-binding protein-related (TAIR:AT5G10660.1); Has 1807 Blast hits to 1807 proteins in 277 species: Archae - 0; Bacteria - 0; Metazoa - 736; Fungi - 347; Plants - 385; Viruses - 0; Other Eukaryotes - 339 (source: NCBI BLink). )

HSP 1 Score: 76.3 bits (186), Expect = 7.9e-14
Identity = 152/482 (31.54%), Postives = 228/482 (47.30%), Query Frame = 0

Query: 19  TAPSSITVPNHTSTKRTPKSSPRAPSTDHQKLSSLPSSSSSSAPSLSTHAEKHVPNYLKS 78
           T+ ++   P++ +  RT KS  R P    Q LS+  S   SS        EK +PNYLK 
Sbjct: 4   TSSTTKAKPDNQNLSRT-KSLGRKPK---QPLSTASSEDGSS----DQKPEKPLPNYLKP 63

Query: 79  TASSRHDHSSSFKPIKKTTTTTAAPAPIPEGKPSLNRRRSFDKPPPTAPRLDKPFRSPGP 138
           T SSR D   + K +KK   +        +    L RRRSFD+PP +   L  P  S  P
Sbjct: 64  TISSRPD---AGKLLKKNNNSL-------DDNQKLLRRRSFDRPPSS---LTSPSTSASP 123

Query: 139 R-NRAAHV-PVRSSSFGAKP--SSTTILSSSSKVLERSSSKNSKVGGG---GKPLQPVHT 198
           R  ++ +V P RS    A P     T L SSS       S+N   GG      P+ P  +
Sbjct: 124 RIQKSLNVSPSRSRDRPAVPREKPVTALRSSS----FHGSRNVPKGGNTAKSPPVAPKKS 183

Query: 199 MIRSSSNVKKSLRRDSSNAAAAAAKNAVEHVD-RSPSFVLGMNEEELKKIEYELDPFLPD 258
            + SSS   KS +  S N     A +    +D  S S     ++EE+ K+E        D
Sbjct: 184 GLNSSSTSSKSKKEGSENVRIKKASDKEIALDSASMSSAQEDHQEEILKVE-------SD 243

Query: 259 PMPELDKQLTTTDQVEKKQVLDKEEVEAEKKLHHQQQPTNDTEISKVVVS--NKEVAETD 318
            +   D  +      ++++ + ++ V+A + +  + + +  T ++  V    N  VAE +
Sbjct: 244 HLQVSDHDIEEPKYEKEEKEVQEKVVQANESVEEKAESSGPTPVASPVGKDCNAVVAELE 303

Query: 319 AKSPVQEDENRERNQEEEEEEAKEEEKPERIVVEKEDVVAVVVTSEENTNNNNEEKMV-T 378
            K    ED+  E+ +E +E++  +  K E    E+EDV   +  +E     + E K V +
Sbjct: 304 EKLIKNEDDIEEKTEEMKEQDNNQANKSE----EEEDVKKKIDENETPEKVDTESKEVES 363

Query: 379 EEKSETETDPEVSEGGEGAVVLDQKEAQDGQSFKEENPNSSSPAKAETPEKETEATEKMA 438
            E++  E + EV E G+  V  ++KE +     K +  +     + E  EK     EK  
Sbjct: 364 VEETTQEKEEEVKEEGKERVEEEEKEKE-----KVKEDDQKEKVEEEEKEKVKGDEEKEK 423

Query: 439 VAAAEKAAGKPRQGGPGRKESPAMYNHVIEETASKLLEK-RKNKVRALAGAFQTVIDYES 489
           V   E A GK ++   G+KESP+ YN VI   ASK+ E  RKNKV ALAGAFQTVIDYE+
Sbjct: 424 VKEEESAEGKKKEVVKGKKESPSAYNDVI---ASKMQENPRKNKVLALAGAFQTVIDYET 441

BLAST of Lag0005144 vs. TAIR 10
Match: AT5G10660.1 (calmodulin-binding protein-related )

HSP 1 Score: 52.4 bits (124), Expect = 1.2e-06
Identity = 148/480 (30.83%), Postives = 202/480 (42.08%), Query Frame = 0

Query: 25  TVPNHTSTKRTPKSSPRAPSTDHQKLSSLPSSSSSSAPSLSTHAE------KHVPNYLKS 84
           T  + T TKR  K+  R+ S     L   P   SSS P  +          K +PNYLK 
Sbjct: 3   TTSSTTKTKRDNKNLTRSKS-----LGRKPKPVSSSEPEANADGSDRKTIGKPLPNYLKP 62

Query: 85  TASSRHDHSSSFKPIKKTTTTTAAPAPIPEGKPSLNRRRSFDKPPP--TAPRLDKPFRSP 144
           T SSR D      P+K      A      E    L RRRSFD PP   T+P      RS 
Sbjct: 63  TISSRPD------PVKFLRKNNAV-----EENQKLLRRRSFDHPPSSLTSPSTSSAHRS- 122

Query: 145 GPRNRAAHVPVRSSSFGAKPSSTTILSSSSKVLERSSSKNSKVGGGGKPLQPVHTMIRSS 204
                 AH  +R      +    T L S+S      SS+    G       PV +  R S
Sbjct: 123 -LNTSPAHPHLRDKPAVPREKPVTGLRSTS---FHGSSRGGLRGSSTVKSPPVAS--RGS 182

Query: 205 SNVKKS-LRRDSSN------AAAAAAKNAVEHVDRSPSFVLGMNEEELKKIEYELDPFLP 264
           S VKKS L  +SS+      +     K++ + +    S +   +E+E + ++ E D  + 
Sbjct: 183 SGVKKSGLSGNSSSKSKKEGSGNVPKKSSGKEISPDSSPLASAHEDEEEIVKVETDVHIS 242

Query: 265 DPMPELDKQLTTTDQVEKKQVLDKEEVEAEKKLHHQQQPTNDTEISKVVVSNKEVAETDA 324
           D   E  ++       +K Q    +E   EK+          TE  K  + +++ +    
Sbjct: 243 DHGEEPKEE-------DKDQFAQPDESGEEKE---TSPVAASTEEQKGELIDEDKSTEQI 302

Query: 325 KSPVQEDENRERNQEEEEEEAKEEEKPERIVVEKEDVVAVVVTSEENTNNNNEEKMVTEE 384
           + P +E EN E N  EEEEE K++   E    E  + VA        T + NE   V E 
Sbjct: 303 EEP-KEPENIEENNSEEEEEVKKKSDDE----ENSETVA-------TTTDMNEAVNVEES 362

Query: 385 KSETETDPEVSEGGEGAVVLDQKEAQDGQSFKEENPNSSSPAKAETPEKETEATEKMAVA 444
           K E + + EV E             ++G+         SS AK ET       TE MA  
Sbjct: 363 KEEEKEEAEVKE-------------EEGE---------SSAAKEET-------TETMAQV 405

Query: 445 AAEKAAGKPRQGGPGRKESPAMYNHVIEETASKLLE-KRKNKVRALAGAFQTVIDYESSS 489
                 G   +   G+KESP  YN VI   ASK+ E  +KNKV ALAGAFQTVIDYE+++
Sbjct: 423 EELPEEGTKNEVVQGKKESPTAYNDVI---ASKMQENSKKNKVLALAGAFQTVIDYETAA 405

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
KAG6598809.12.4e-13766.14hypothetical protein SDJN03_08587, partial [Cucurbita argyrosperma subsp. sorori... [more]
XP_023546370.13.1e-13766.21cell wall protein RBR3-like [Cucurbita pepo subsp. pepo][more]
KAG7029749.14.0e-13766.14hypothetical protein SDJN02_08091, partial [Cucurbita argyrosperma subsp. argyro... [more]
XP_022997522.15.3e-13766.54microtubule-associated protein RP/EB family member 1-like [Cucurbita maxima][more]
XP_022962060.19.0e-13766.34neurofilament medium polypeptide-like [Cucurbita moschata][more]
Match NameE-valueIdentityDescription
Match NameE-valueIdentityDescription
A0A6J1K5992.5e-13766.54microtubule-associated protein RP/EB family member 1-like OS=Cucurbita maxima OX... [more]
A0A6J1HE164.3e-13766.34neurofilament medium polypeptide-like OS=Cucurbita moschata OX=3662 GN=LOC111462... [more]
A0A0A0LS851.3e-12561.15CaM_binding domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_2G378000... [more]
A0A1S3BBU43.8e-12562.35serine/arginine repetitive matrix protein 1 OS=Cucumis melo OX=3656 GN=LOC103488... [more]
A0A5A7VBK46.5e-12562.11Serine/arginine repetitive matrix protein 1 OS=Cucumis melo var. makuwa OX=11946... [more]
Match NameE-valueIdentityDescription
AT5G24880.17.9e-1431.54BEST Arabidopsis thaliana protein match is: calmodulin-binding protein-related (... [more]
AT5G10660.11.2e-0630.83calmodulin-binding protein-related [more]
InterPro
Analysis Name: InterPro Annotations of Sponge gourd (AG-4) v1
Date Performed: 2022-08-01
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
NoneNo IPR availableCOILSCoilCoilcoord: 313..335
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 75..103
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 260..287
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 361..377
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 21..67
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 151..180
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 1..202
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 246..452
NoneNo IPR availablePANTHERPTHR33349EMB|CAB62594.1coord: 1..486
NoneNo IPR availablePANTHERPTHR33349:SF20CALMODULIN-BINDING PROTEIN-LIKE PROTEINcoord: 1..486
IPR012417Calmodulin-binding domain, plantSMARTSM01054CaM_binding_2coord: 363..483
e-value: 4.1E-4
score: 22.2
IPR012417Calmodulin-binding domain, plantPFAMPF07839CaM_bindingcoord: 390..482
e-value: 1.7E-14
score: 54.6

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Lag0005144.1Lag0005144.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
molecular_function GO:0005516 calmodulin binding