Lag0005045 (gene) Sponge gourd (AG‐4) v1

Overview
NameLag0005045
Typegene
OrganismLuffa acutangula (Sponge gourd (AG‐4) v1)
DescriptionRNase H domain-containing protein
Locationchr6: 9956268 .. 9956660 (-)
RNA-Seq ExpressionLag0005045
SyntenyLag0005045
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonCDSpolypeptide
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGTGGGTCGACGTCGCCTGGAATAAAAGCTCCCTAAATACCAGCTATGGGTGGTATATGTGGTTCCCTAATAGCAACACGAGCGAGGATGGATCGAGAAGGGGTGGAAATAGCTTGAATAGCCTGCATGCTGAGGGAAACGCCTTGATTTGGGGCTTGGAATGTGCAGAGAACAAAGGAATTAGGGAAATGTGGGTGAAGTCTGATTCTTTGAGGCTGGTGAATATTGTAAATGACAAAGAGTGCTATCATGTGGACTTTGAGCCAATCCAAGATGCTATTCAGATGATGTATGAGAGATTTAATTCTTGTAATTTGTCATTTGTGAGGAGAGAGCATAATGGAAAAGCACACTGCTTGGCCAGATCTGTAGCCTCTCAATCTTCTATTTAA

mRNA sequence

ATGTGGGTCGACGTCGCCTGGAATAAAAGCTCCCTAAATACCAGCTATGGGTGGTATATGTGGTTCCCTAATAGCAACACGAGCGAGGATGGATCGAGAAGGGGTGGAAATAGCTTGAATAGCCTGCATGCTGAGGGAAACGCCTTGATTTGGGGCTTGGAATGTGCAGAGAACAAAGGAATTAGGGAAATGTGGGTGAAGTCTGATTCTTTGAGGCTGGTGAATATTGTAAATGACAAAGAGTGCTATCATGTGGACTTTGAGCCAATCCAAGATGCTATTCAGATGATGTATGAGAGATTTAATTCTTGTAATTTGTCATTTGTGAGGAGAGAGCATAATGGAAAAGCACACTGCTTGGCCAGATCTGTAGCCTCTCAATCTTCTATTTAA

Coding sequence (CDS)

ATGTGGGTCGACGTCGCCTGGAATAAAAGCTCCCTAAATACCAGCTATGGGTGGTATATGTGGTTCCCTAATAGCAACACGAGCGAGGATGGATCGAGAAGGGGTGGAAATAGCTTGAATAGCCTGCATGCTGAGGGAAACGCCTTGATTTGGGGCTTGGAATGTGCAGAGAACAAAGGAATTAGGGAAATGTGGGTGAAGTCTGATTCTTTGAGGCTGGTGAATATTGTAAATGACAAAGAGTGCTATCATGTGGACTTTGAGCCAATCCAAGATGCTATTCAGATGATGTATGAGAGATTTAATTCTTGTAATTTGTCATTTGTGAGGAGAGAGCATAATGGAAAAGCACACTGCTTGGCCAGATCTGTAGCCTCTCAATCTTCTATTTAA

Protein sequence

MWVDVAWNKSSLNTSYGWYMWFPNSNTSEDGSRRGGNSLNSLHAEGNALIWGLECAENKGIREMWVKSDSLRLVNIVNDKECYHVDFEPIQDAIQMMYERFNSCNLSFVRREHNGKAHCLARSVASQSSI
Homology
BLAST of Lag0005045 vs. NCBI nr
Match: KAG7593976.1 (Ribonuclease H domain [Arabidopsis thaliana x Arabidopsis arenosa])

HSP 1 Score: 69.3 bits (168), Expect = 2.7e-08
Identity = 45/129 (34.88%), Postives = 65/129 (50.39%), Query Frame = 0

Query: 2   WVDVAWNKSSLNTSYGWYMWFPNSNTSEDGSRRGGNSLNSLHAEGNALIWGLECAENKGI 61
           +VD +W +S   +  GW+   PN  T + G+     SL SLH E  ALIW + C   +  
Sbjct: 15  YVDGSWKESDRFSGVGWFCSNPNDRTQDMGAANLRRSLTSLHTEVEALIWAMRCMIGQDK 74

Query: 62  REMWVKSDSLRLVNIVNDKECYHVDFEPIQDAIQMMYERFNSCNLSFVRREHNGKAHCLA 121
           RE+   +D   LV +V+  + +   F    +AIQ   E F+  +LS V R  N KA  LA
Sbjct: 75  REVAFFTDCSDLVKMVSSPQEWPA-FATYLEAIQSDKEEFDVFSLSLVSRNANVKADKLA 134

Query: 122 RSVASQSSI 131
           R+V  Q  +
Sbjct: 135 RNVRIQPQL 142

BLAST of Lag0005045 vs. NCBI nr
Match: XP_010451887.1 (PREDICTED: uncharacterized protein LOC104734079 [Camelina sativa])

HSP 1 Score: 67.8 bits (164), Expect = 8.0e-08
Identity = 40/121 (33.06%), Postives = 63/121 (52.07%), Query Frame = 0

Query: 3   VDVAWNKSSLNTSYGWYMWFPNSNTSEDGSRRGGNSLNSLHAEGNALIWGLECAENKGIR 62
           VD +W  +  N+  GW+     + T   G+R    S++ LH+E  AL+W +EC  ++GI 
Sbjct: 229 VDGSWKSTEQNSGLGWWCGDGENQTFFLGARYQHRSVSPLHSEMEALLWAMECILSRGIV 288

Query: 63  EMWVKSDSLRLVNIVNDKECYHVDFEPIQDAIQMMYERFNSCNLSFVRREHNGKAHCLAR 122
               ++DS  L+++V D E +   F  + D  Q++   F    LS + R  N KA CLA 
Sbjct: 289 CQRFETDSAELISMVQDPEEWPA-FSALLDEFQVLSASFPHFTLSKIPRTVNVKADCLAH 348

Query: 123 S 124
           S
Sbjct: 349 S 348

BLAST of Lag0005045 vs. NCBI nr
Match: KAG7571790.1 (Reverse transcriptase domain [Arabidopsis suecica])

HSP 1 Score: 67.4 bits (163), Expect = 1.0e-07
Identity = 41/122 (33.61%), Postives = 62/122 (50.82%), Query Frame = 0

Query: 3    VDVAWNKSSLNTSYGWYMWFPNSNTSEDGSRRGGNSLNSLHAEGNALIWGLECAENKGIR 62
            +D +W +S   +  GW+  +PN    + G+     SL SLH E  ALIW + C   +  R
Sbjct: 1030 LDGSWKESDCFSGLGWFCSYPNEEAHDMGAANTRRSLTSLHTEVEALIWAMRCMIGQDKR 1089

Query: 63   EMWVKSDSLRLVNIVNDKECYHVDFEPIQDAIQMMYERFNSCNLSFVRREHNGKAHCLAR 122
            E+   +D   LV +V+  + + V F    +AIQ   E F+  +L  + R  N KA  LAR
Sbjct: 1090 EVAFLTDCSDLVKMVSSPQEWPV-FTTYLEAIQSDKEEFDFFSLPLISRNLNVKADKLAR 1149

Query: 123  SV 125
            +V
Sbjct: 1150 NV 1150

BLAST of Lag0005045 vs. NCBI nr
Match: KAG7585995.1 (Reverse transcriptase zinc-binding domain [Arabidopsis thaliana x Arabidopsis arenosa])

HSP 1 Score: 65.1 bits (157), Expect = 5.2e-07
Identity = 40/123 (32.52%), Postives = 62/123 (50.41%), Query Frame = 0

Query: 2    WVDVAWNKSSLNTSYGWYMWFPNSNTSEDGSRRGGNSLNSLHAEGNALIWGLECAENKGI 61
            ++D +W ++   +  GW+  +PN    + G+     SL SLH E  ALIW + C   +  
Sbjct: 1004 FLDGSWKENDCFSGLGWFCSYPNEEMHDMGAANTRRSLTSLHTEVEALIWAMRCMIGQDK 1063

Query: 62   REMWVKSDSLRLVNIVNDKECYHVDFEPIQDAIQMMYERFNSCNLSFVRREHNGKAHCLA 121
            RE+   +D   LV +V+    +   F    +AIQ   E F+  +LS + R  N KA  LA
Sbjct: 1064 REVAFLTDCSDLVKMVSSPHEWPA-FATYLEAIQSDKEEFDFFSLSLISRNLNVKADKLA 1123

Query: 122  RSV 125
            R+V
Sbjct: 1124 RNV 1125

BLAST of Lag0005045 vs. NCBI nr
Match: KAG7539157.1 (Ribonuclease H domain [Arabidopsis suecica])

HSP 1 Score: 64.7 bits (156), Expect = 6.7e-07
Identity = 43/129 (33.33%), Postives = 63/129 (48.84%), Query Frame = 0

Query: 2   WVDVAWNKSSLNTSYGWYMWFPNSNTSEDGSRRGGNSLNSLHAEGNALIWGLECAENKGI 61
           +VD +W +S   +   W+   PN  T + G+     SL SLH E  ALIW + C   +  
Sbjct: 99  YVDGSWKESDRFSGVAWFCSNPNDGTQDMGAANLRRSLTSLHTEVEALIWAMRCMIGQDK 158

Query: 62  REMWVKSDSLRLVNIVNDKECYHVDFEPIQDAIQMMYERFNSCNLSFVRREHNGKAHCLA 121
           RE+   +D   LV +V   + +        +AIQ   E F+  +LS V R  N KA  LA
Sbjct: 159 REVVFFTDCSDLVKMVYSPQEWPA-LATYLEAIQSDKEEFDVFSLSLVSRNANVKADKLA 218

Query: 122 RSVASQSSI 131
           R+V +Q  +
Sbjct: 219 RNVRTQPQL 226

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
KAG7593976.12.7e-0834.88Ribonuclease H domain [Arabidopsis thaliana x Arabidopsis arenosa][more]
XP_010451887.18.0e-0833.06PREDICTED: uncharacterized protein LOC104734079 [Camelina sativa][more]
KAG7571790.11.0e-0733.61Reverse transcriptase domain [Arabidopsis suecica][more]
KAG7585995.15.2e-0732.52Reverse transcriptase zinc-binding domain [Arabidopsis thaliana x Arabidopsis ar... [more]
KAG7539157.16.7e-0733.33Ribonuclease H domain [Arabidopsis suecica][more]
Match NameE-valueIdentityDescription
InterPro
Analysis Name: InterPro Annotations of Sponge gourd (AG-4) v1
Date Performed: 2022-08-01
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR002156Ribonuclease H domainPFAMPF13456RVT_3coord: 3..123
e-value: 3.7E-22
score: 78.5
IPR036397Ribonuclease H superfamilyGENE3D3.30.420.10coord: 1..129
e-value: 5.9E-16
score: 60.6
NoneNo IPR availablePANTHERPTHR47723OS05G0353850 PROTEINcoord: 34..127
IPR012337Ribonuclease H-like superfamilySUPERFAMILY53098Ribonuclease H-likecoord: 2..127

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Lag0005045.1Lag0005045.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
molecular_function GO:0003676 nucleic acid binding
molecular_function GO:0004523 RNA-DNA hybrid ribonuclease activity