Lag0004915 (gene) Sponge gourd (AG‐4) v1

BLAST of Lag0004915 vs. NCBI nr
Match: XP_008438607.1 (PREDICTED: ATP sulfurylase 1, chloroplastic [Cucumis melo])

HSP 1 Score: 143.7 bits (361), Expect = 1.2e-30
Identity = 77/99 (77.78%), Postives = 84/99 (84.85%), Query Frame = 0

Query: 43  MATTTTRFTKTPSPFRSIQKTFQTHLGAPIK----SSKVKKTYLRLWVSTALIEPDGERL 102
           MA+  TRFTKTPSPF SIQ+T  THLGAPIK    +SK KKT+LRL VS  LIEPDG RL
Sbjct: 1   MASMATRFTKTPSPFHSIQRTSYTHLGAPIKVSIATSKSKKTHLRLRVSAGLIEPDGGRL 60

Query: 103 VEVLVKEPLRGLKKKGALSLPRIKLSSIDIQWVHVLSEG 138
           VE+LV+EPLRGLKKK ALSLPRI+LSSIDIQWVHVLSEG
Sbjct: 61  VELLVEEPLRGLKKKEALSLPRIELSSIDIQWVHVLSEG 99

BLAST of Lag0004915 vs. NCBI nr
Match: KAA0049323.1 (ATP sulfurylase 1 [Cucumis melo var. makuwa] >TYK17235.1 ATP sulfurylase 1 [Cucumis melo var. makuwa])

HSP 1 Score: 142.1 bits (357), Expect = 3.5e-30
Identity = 76/99 (76.77%), Postives = 83/99 (83.84%), Query Frame = 0

Query: 43  MATTTTRFTKTPSPFRSIQKTFQTHLGAPIK----SSKVKKTYLRLWVSTALIEPDGERL 102
           MA+  TRFTKTPSPF SIQ+T  TH GAPIK    +SK KKT+LRL VS  LIEPDG RL
Sbjct: 1   MASMATRFTKTPSPFHSIQRTSYTHFGAPIKVSIATSKSKKTHLRLRVSAGLIEPDGGRL 60

Query: 103 VEVLVKEPLRGLKKKGALSLPRIKLSSIDIQWVHVLSEG 138
           VE+LV+EPLRGLKKK ALSLPRI+LSSIDIQWVHVLSEG
Sbjct: 61  VELLVEEPLRGLKKKEALSLPRIELSSIDIQWVHVLSEG 99

BLAST of Lag0004915 vs. NCBI nr
Match: XP_004134113.1 (ATP sulfurylase 1, chloroplastic [Cucumis sativus] >KGN56933.1 hypothetical protein Csa_009913 [Cucumis sativus])

HSP 1 Score: 140.2 bits (352), Expect = 1.3e-29
Identity = 74/99 (74.75%), Postives = 83/99 (83.84%), Query Frame = 0

Query: 43  MATTTTRFTKTPSPFRSIQKTFQTHLGAPIK----SSKVKKTYLRLWVSTALIEPDGERL 102
           MA+  TRFT TPSPF SIQ+T  THLGAP+K    +SK KKT+LRL VS  LIEPDG +L
Sbjct: 1   MASMATRFTNTPSPFHSIQRTSYTHLGAPVKVSISTSKSKKTHLRLRVSAGLIEPDGGKL 60

Query: 103 VEVLVKEPLRGLKKKGALSLPRIKLSSIDIQWVHVLSEG 138
           VE+LV+EPLRGLKKK ALSLPRI+LSSIDIQWVHVLSEG
Sbjct: 61  VELLVEEPLRGLKKKEALSLPRIELSSIDIQWVHVLSEG 99

BLAST of Lag0004915 vs. NCBI nr
Match: XP_038895326.1 (ATP sulfurylase 1, chloroplastic-like [Benincasa hispida])

HSP 1 Score: 137.9 bits (346), Expect = 6.6e-29
Identity = 74/99 (74.75%), Postives = 81/99 (81.82%), Query Frame = 0

Query: 43  MATTTTRFTKTPSPFRSIQKTFQTHLGAPIK----SSKVKKTYLRLWVSTALIEPDGERL 102
           MA+  TRFTKTPSPF SIQ+T  THLGAPIK    SSK KK++ RL VS  LIEPDG RL
Sbjct: 1   MASMATRFTKTPSPFHSIQRTSYTHLGAPIKVSIASSKSKKSHFRLRVSAGLIEPDGGRL 60

Query: 103 VEVLVKEPLRGLKKKGALSLPRIKLSSIDIQWVHVLSEG 138
           VE+LV+EPLR LKKK ALSLPRI+LSSIDIQW HVLSEG
Sbjct: 61  VELLVEEPLRALKKKEALSLPRIELSSIDIQWAHVLSEG 99

BLAST of Lag0004915 vs. NCBI nr
Match: KAG6597410.1 (ATP sulfurylase 1, chloroplastic, partial [Cucurbita argyrosperma subsp. sororia] >KAG7028868.1 ATP sulfurylase 1, chloroplastic, partial [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 136.7 bits (343), Expect = 1.5e-28
Identity = 75/101 (74.26%), Postives = 79/101 (78.22%), Query Frame = 0

Query: 43  MATTTTRFTKTPSPFRSIQKTFQTHLGAPIK------SSKVKKTYLRLWVSTALIEPDGE 102
           MA+T TRF K PSPF SIQ+T  THLGAPIK      S K KK  LRL VS  LIEPDG 
Sbjct: 1   MASTATRFIKAPSPFHSIQRTSYTHLGAPIKISLPAGSFKAKKANLRLRVSAGLIEPDGG 60

Query: 103 RLVEVLVKEPLRGLKKKGALSLPRIKLSSIDIQWVHVLSEG 138
           RLVE+LV+EPLRGLKKK ALSLPRI LSSIDIQWVHVLSEG
Sbjct: 61  RLVELLVEEPLRGLKKKEALSLPRISLSSIDIQWVHVLSEG 101

BLAST of Lag0004915 vs. ExPASy Swiss-Prot
Match: O23324 (ATP-sulfurylase 3, chloroplastic OS=Arabidopsis thaliana OX=3702 GN=APS3 PE=1 SV=1)

HSP 1 Score: 67.8 bits (164), Expect = 1.1e-10
Identity = 39/99 (39.39%), Postives = 56/99 (56.57%), Query Frame = 0

Query: 43  MATTTTRFTKTPS----PFRSIQKTFQTHLGAPIKSSKVKKTYLRLWVSTALIEPDGERL 102
           MA+ +T F K  S    P     K+          S+   ++   + V   LIEPDG +L
Sbjct: 1   MASMSTVFPKPTSFISQPLTKSHKSDSVTTSISFPSNSKTRSLRTISVRAGLIEPDGGKL 60

Query: 103 VEVLVKEPLRGLKKKGALSLPRIKLSSIDIQWVHVLSEG 138
           V+++V EP R  KK  A  LPR++L++ID+QW+HVLSEG
Sbjct: 61  VDLVVPEPRRREKKHEAADLPRVRLTAIDLQWMHVLSEG 99

BLAST of Lag0004915 vs. ExPASy Swiss-Prot
Match: Q9LIK9 (ATP sulfurylase 1, chloroplastic OS=Arabidopsis thaliana OX=3702 GN=APS1 PE=1 SV=1)

HSP 1 Score: 63.5 bits (153), Expect = 2.1e-09
Identity = 39/98 (39.80%), Postives = 57/98 (58.16%), Query Frame = 0

Query: 43  MATTTTRFTKTPSPFRSIQKTFQTHLGAPIKSSKVKKTYLRLWVST---ALIEPDGERLV 102
           MA+     +KTP   + + K+   +   P  +       LR  V +    LI PDG +LV
Sbjct: 1   MASMAAVLSKTPFLSQPLTKS-SPNSDLPFAAVSFPSKSLRRRVGSIRAGLIAPDGGKLV 60

Query: 103 EVLVKEPLRGLKKKGALSLPRIKLSSIDIQWVHVLSEG 138
           E++V+EP R  KK  A  LPR++L++ID+QW+HVLSEG
Sbjct: 61  ELIVEEPKRREKKHEAADLPRVELTAIDLQWMHVLSEG 97

BLAST of Lag0004915 vs. ExPASy Swiss-Prot
Match: Q43870 (ATP sulfurylase 2 OS=Arabidopsis thaliana OX=3702 GN=APS2 PE=1 SV=1)

HSP 1 Score: 62.0 bits (149), Expect = 6.0e-09
Identity = 27/65 (41.54%), Postives = 47/65 (72.31%), Query Frame = 0

Query: 73  KSSKVKKTYLRLWVSTALIEPDGERLVEVLVKEPLRGLKKKGALSLPRIKLSSIDIQWVH 132
           K +   ++  ++ V ++LI+PDG  LVE++V E   G+KK  + ++P++KL+ ID++WVH
Sbjct: 47  KRNLTMQSVSKMTVKSSLIDPDGGELVELIVPETEIGVKKAESETMPKVKLNQIDLEWVH 106

Query: 133 VLSEG 138
           V+SEG
Sbjct: 107 VISEG 111

BLAST of Lag0004915 vs. ExPASy Swiss-Prot
Match: Q9S7D8 (ATP sulfurylase 4, chloroplastic OS=Arabidopsis thaliana OX=3702 GN=APS4 PE=2 SV=1)

HSP 1 Score: 55.8 bits (133), Expect = 4.3e-07
Identity = 38/90 (42.22%), Postives = 53/90 (58.89%), Query Frame = 0

Query: 55  SPFRSIQKTFQTHLG--APIKSSKV----KKTYLRLWVSTALIEPDGERLVEVLVKEPLR 114
           SPFRS       H    AP   S V    + +   L V + LIEPDG +L+ ++V+E  R
Sbjct: 12  SPFRSSPLIHNHHASRYAPGSISVVSLPRQVSRRGLSVKSGLIEPDGGKLMNLVVEESRR 71

Query: 115 GLKKKGALSLP-RIKLSSIDIQWVHVLSEG 138
            + K  A ++P RIKL+ +D++WVHVLSEG
Sbjct: 72  RVMKHEAETVPARIKLNRVDLEWVHVLSEG 101