Lag0004881 (gene) Sponge gourd (AG‐4) v1

Overview
NameLag0004881
Typegene
OrganismLuffa acutangula (Sponge gourd (AG‐4) v1)
DescriptionCysteine dioxygenase
Locationchr6: 8061762 .. 8062602 (-)
RNA-Seq ExpressionLag0004881
SyntenyLag0004881
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: polypeptideexonCDS
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGAAAGCTGCAGATGTTGGAATTGAAGAGTTGGAAGAGCCAGAATCGTCAATTGTGAGCCCCAGGGGATTGATTTACGCCAAGGGACTTCCAGAAATAACATACATCCACATCCACGAATGCGACTTGTTCTCTGTAAGTTGAAATAAAATCAAAATTTTAGATAAATTGAAATGTTTTTAATTTGGGTTTGGATCTTGTGTATGTGTAGATTGGAATATTCTGTTTCCCTGAAGGCGGGGCGCTGCCGTTGCACGACCATCCGGGGATGACGGTGATGAGCAAGGTTCTGTATGGGTCGGTTCACGTCAAGGCGTACGATTGGATCAAAATGTACGGCTCCGTCGGTGGGCGAACGTGTGAGGAAATTTTATTGATTGGTTTTTGAGTTGAGAAGAGTAACGCCCCACCCAGGATTCAGAATTCAGATTCGACACCTGATCTGGTATTCACCTACATCTCCTACGACCTGGGGAATTCGGATTTCGAATCCTGGATGTGGAGCGTTACATGGAGTTTGGTTCATTTGGTGAGTTTTATTTTATGAATTGGTTTAAAATGTGTAATGATGTAAATGGCAGTTGGGTTGGGAGGGAAGGTGATGGATGGAGTTTGGAGTGCGGGATGTGAAGCGAGGGCTCTGTTTCCGAGGAGTGGAGGGAACATTCATTCGTTTAGGGCGATGTCGGCGTGTGCCATTTTGGATGTCTTGTCTCCGCCGTATTCTAAAGAGCTTGGAAGGCCTTCCACTTACTTCTCCGACTTACGCATCCCAACTCTTCCTGGTGATTCTAATTACTCTCTTCCTTCTAATCTTTCTCTTCATATTTCTTCTATTTAG

mRNA sequence

ATGAAAGCTGCAGATGTTGGAATTGAAGAGTTGGAAGAGCCAGAATCGTCAATTGTGAGCCCCAGGGGATTGATTTACGCCAAGGGACTTCCAGAAATAACATACATCCACATCCACGAATGCGACTTGTTCTCTATTGGAATATTCTGTTTCCCTGAAGGCGGGGCGCTGCCGTTGCACGACCATCCGGGGATGACGGTGATGAGCAAGGTTCTGTATGGGTCGGTTCACGTCAAGGCGTACGATTGGATCAAAATGTACGGCTCCGTCGGTGGGCGAACGTTTGGGTTGGGAGGGAAGGTGATGGATGGAGTTTGGAGTGCGGGATGTGAAGCGAGGGCTCTGTTTCCGAGGAGTGGAGGGAACATTCATTCGTTTAGGGCGATGTCGGCGTGTGCCATTTTGGATGTCTTGTCTCCGCCGTATTCTAAAGAGCTTGGAAGGCCTTCCACTTACTTCTCCGACTTACGCATCCCAACTCTTCCTGGTGATTCTAATTACTCTCTTCCTTCTAATCTTTCTCTTCATATTTCTTCTATTTAG

Coding sequence (CDS)

ATGAAAGCTGCAGATGTTGGAATTGAAGAGTTGGAAGAGCCAGAATCGTCAATTGTGAGCCCCAGGGGATTGATTTACGCCAAGGGACTTCCAGAAATAACATACATCCACATCCACGAATGCGACTTGTTCTCTATTGGAATATTCTGTTTCCCTGAAGGCGGGGCGCTGCCGTTGCACGACCATCCGGGGATGACGGTGATGAGCAAGGTTCTGTATGGGTCGGTTCACGTCAAGGCGTACGATTGGATCAAAATGTACGGCTCCGTCGGTGGGCGAACGTTTGGGTTGGGAGGGAAGGTGATGGATGGAGTTTGGAGTGCGGGATGTGAAGCGAGGGCTCTGTTTCCGAGGAGTGGAGGGAACATTCATTCGTTTAGGGCGATGTCGGCGTGTGCCATTTTGGATGTCTTGTCTCCGCCGTATTCTAAAGAGCTTGGAAGGCCTTCCACTTACTTCTCCGACTTACGCATCCCAACTCTTCCTGGTGATTCTAATTACTCTCTTCCTTCTAATCTTTCTCTTCATATTTCTTCTATTTAG

Protein sequence

MKAADVGIEELEEPESSIVSPRGLIYAKGLPEITYIHIHECDLFSIGIFCFPEGGALPLHDHPGMTVMSKVLYGSVHVKAYDWIKMYGSVGGRTFGLGGKVMDGVWSAGCEARALFPRSGGNIHSFRAMSACAILDVLSPPYSKELGRPSTYFSDLRIPTLPGDSNYSLPSNLSLHISSI
Homology
BLAST of Lag0004881 vs. NCBI nr
Match: XP_038886772.1 (plant cysteine oxidase 4-like [Benincasa hispida])

HSP 1 Score: 274.6 bits (701), Expect = 5.9e-70
Identity = 133/163 (81.60%), Postives = 140/163 (85.89%), Query Frame = 0

Query: 1   MKAADVGIEELEEPESSIVSPRGLIYAKGLPEITYIHIHECDLFSIGIFCFPEGGALPLH 60
           +KA DVGIEELEE E SI+SPRGLIYAKG+ EITYIHIHECD FSIGIFC PEGG LPLH
Sbjct: 38  IKAIDVGIEELEEAECSIISPRGLIYAKGVSEITYIHIHECDFFSIGIFCLPEGGELPLH 97

Query: 61  DHPGMTVMSKVLYGSVHVKAYDWIKM-YGSVGGRTFGLGGKVMDGVWSAGCEARALFPRS 120
           DHPGMTVMSK+LYGSVHVKAYDWIKM   S   RTFGLG KV+D VWSA  EARALFP S
Sbjct: 98  DHPGMTVMSKLLYGSVHVKAYDWIKMQISSSTDRTFGLGKKVVDKVWSAPSEARALFPAS 157

Query: 121 GGNIHSFRAMSACAILDVLSPPYSKELGRPSTYFSDLRIPTLP 163
           GGNIHSFRA S CAILDVLSPPYS+ LGRPSTYFSD  +PTLP
Sbjct: 158 GGNIHSFRAKSGCAILDVLSPPYSQHLGRPSTYFSDFPLPTLP 200

BLAST of Lag0004881 vs. NCBI nr
Match: KAE8056931.1 (hypothetical protein FH972_013663 [Carpinus fangiana])

HSP 1 Score: 229.9 bits (585), Expect = 1.7e-56
Identity = 111/165 (67.27%), Postives = 128/165 (77.58%), Query Frame = 0

Query: 2   KAADVGIEEL---EEPESSIVSPRGLIYAKGLPEITYIHIHECDLFSIGIFCFPEGGALP 61
           KA D+GI+E    E P SS  S  GLI  +G+  ITYIHIHECD FSIG+FCFP G   P
Sbjct: 45  KAIDLGIDEFGFCESPPSSPTSVEGLIRGQGVCNITYIHIHECDNFSIGVFCFPAGRIFP 104

Query: 62  LHDHPGMTVMSKVLYGSVHVKAYDWIKMYGSVGGRTFGLGGKVMDGVWSAGCEARALFPR 121
           LHDHP MTV+SK+LYGSV+VKAYDW+K+  S G RT+GL G V+DG+  A CEA  LFP 
Sbjct: 105 LHDHPRMTVLSKLLYGSVYVKAYDWVKVVNS-GCRTYGLAGSVVDGILKAPCEASVLFPG 164

Query: 122 SGGNIHSFRAMSACAILDVLSPPYSKELGRPSTYFSDLRIPTLPG 164
           SGGNIHSF A++ CAILDVLSPPYS+ELGRPSTYFSD  IP+LPG
Sbjct: 165 SGGNIHSFAALTPCAILDVLSPPYSEELGRPSTYFSDFPIPSLPG 208

BLAST of Lag0004881 vs. NCBI nr
Match: XP_018858027.1 (plant cysteine oxidase 1-like [Juglans regia] >KAF5456140.1 hypothetical protein F2P56_025650 [Juglans regia])

HSP 1 Score: 229.9 bits (585), Expect = 1.7e-56
Identity = 108/164 (65.85%), Postives = 129/164 (78.66%), Query Frame = 0

Query: 3   AADVGIEEL---EEPESSIVSPRGLIYAKGLPEITYIHIHECDLFSIGIFCFPEGGALPL 62
           A D+GI+E      P +S  S  GLI  +G+  ITYIHIHECD FS+G+FCFP GG LPL
Sbjct: 46  AIDLGIDEFGLCGSPPTSPKSVEGLICGQGVRNITYIHIHECDNFSMGVFCFPAGGVLPL 105

Query: 63  HDHPGMTVMSKVLYGSVHVKAYDWIKMYGSVGGRTFGLGGKVMDGVWSAGCEARALFPRS 122
           HDHPGMTV +K+LYGSV+VKAYDW+K+  S G +TFGL  + +DG++ A CEA  LFPRS
Sbjct: 106 HDHPGMTVFTKLLYGSVYVKAYDWVKVMNS-GCQTFGLASRAVDGIFKAPCEASVLFPRS 165

Query: 123 GGNIHSFRAMSACAILDVLSPPYSKELGRPSTYFSDLRIPTLPG 164
           GGNIHSF A++ CAILDVLSPPYS+ELGRPSTYFSD  IP+LPG
Sbjct: 166 GGNIHSFTALTPCAILDVLSPPYSEELGRPSTYFSDFHIPSLPG 208

BLAST of Lag0004881 vs. NCBI nr
Match: XP_024174882.1 (plant cysteine oxidase 1 [Rosa chinensis] >PRQ21751.1 putative cysteamine dioxygenase [Rosa chinensis])

HSP 1 Score: 228.4 bits (581), Expect = 4.9e-56
Identity = 104/165 (63.03%), Postives = 129/165 (78.18%), Query Frame = 0

Query: 2   KAADVGIEE---LEEPESSIVSPRGLIYAKGLPEITYIHIHECDLFSIGIFCFPEGGALP 61
           +A D GI+E      P S+  S +GLI  +G+ +ITYIH++EC+ FSIG+FCFP GG LP
Sbjct: 44  EAIDFGIDEHGLCGSPSSTPKSDKGLICGQGISQITYIHVYECEQFSIGVFCFPAGGMLP 103

Query: 62  LHDHPGMTVMSKVLYGSVHVKAYDWIKMYGSVGGRTFGLGGKVMDGVWSAGCEARALFPR 121
           LHDHPGMTV SK+LYGS +VKAYDW+K+  S G  TFGLGGKV+DG+ S  CE   LFP+
Sbjct: 104 LHDHPGMTVFSKLLYGSCYVKAYDWVKVESSTGFPTFGLGGKVLDGLMSVPCETSVLFPK 163

Query: 122 SGGNIHSFRAMSACAILDVLSPPYSKELGRPSTYFSDLRIPTLPG 164
           SGGNIH+F A++ CAILDVL+PPYS+ELGRPSTYF +  IP+LPG
Sbjct: 164 SGGNIHAFTALTPCAILDVLTPPYSEELGRPSTYFMEFPIPSLPG 208

BLAST of Lag0004881 vs. NCBI nr
Match: XP_009373036.1 (PREDICTED: plant cysteine oxidase 1-like isoform X2 [Pyrus x bretschneideri])

HSP 1 Score: 228.0 bits (580), Expect = 6.4e-56
Identity = 107/165 (64.85%), Postives = 127/165 (76.97%), Query Frame = 0

Query: 2   KAADVGIEEL---EEPESSIVSPRGLIYAKGLPEITYIHIHECDLFSIGIFCFPEGGALP 61
           +A D GI+E      P SS  S +GL+  +G+ +ITYIH+HECD FSIG+FCFP G  LP
Sbjct: 45  EAIDFGIDEFGSGRSPSSSPRSCKGLVSGQGISQITYIHVHECDHFSIGVFCFPAGATLP 104

Query: 62  LHDHPGMTVMSKVLYGSVHVKAYDWIKMYGSVGGRTFGLGGKVMDGVWSAGCEARALFPR 121
           LHDHPGMTV +K+LYGSV+VK+YDWIK+  S   RTFGL GKV DGV S+ CE R LFPR
Sbjct: 105 LHDHPGMTVFTKLLYGSVYVKSYDWIKVETSSSFRTFGLAGKVFDGVISSPCETRVLFPR 164

Query: 122 SGGNIHSFRAMSACAILDVLSPPYSKELGRPSTYFSDLRIPTLPG 164
           SGGNIHSF A++ CAILDVL+PPYS + GRPSTYF D  IP+LPG
Sbjct: 165 SGGNIHSFTAVTPCAILDVLTPPYSDDHGRPSTYFFDFPIPSLPG 209

BLAST of Lag0004881 vs. ExPASy Swiss-Prot
Match: Q9LXG9 (Plant cysteine oxidase 1 OS=Arabidopsis thaliana OX=3702 GN=PCO1 PE=1 SV=1)

HSP 1 Score: 158.3 bits (399), Expect = 8.1e-38
Identity = 75/171 (43.86%), Postives = 103/171 (60.23%), Query Frame = 0

Query: 1   MKAADVGIEELEEPESSIVSPRGLIYAKGLPEITYIHIHECDLFSIGIFCFPEGGALPLH 60
           MK  DVG+     P      P   + A+  P ITY+H+H+CD FSIGIFC P  G +PLH
Sbjct: 93  MKPEDVGL----TPTMPYFRPNSGVEARSSPPITYLHLHQCDQFSIGIFCLPPSGVIPLH 152

Query: 61  DHPGMTVMSKVLYGSVHVKAYDWIKMYGSVGGRTFGLGGKVMDGVWSAGCEARALFPRSG 120
           +HPGMTV SK+L+G++H+K+YDW+        +T  L    +D  ++A C A  L+P  G
Sbjct: 153 NHPGMTVFSKLLFGTMHIKSYDWVVDAPMRDSKT-RLAKLKVDSTFTAPCNASILYPEDG 212

Query: 121 GNIHSFRAMSACAILDVLSPPYSKELGRPSTYFSDLRIPTLPGDSNYSLPS 172
           GN+H F A++ACA+LDVL PPY    GR  TYF +  +  L  + +  L S
Sbjct: 213 GNMHRFTAITACAVLDVLGPPYCNPEGRHCTYFLEFPLDKLSSEDDDVLSS 258

BLAST of Lag0004881 vs. ExPASy Swiss-Prot
Match: Q9SJI9 (Plant cysteine oxidase 4 OS=Arabidopsis thaliana OX=3702 GN=PCO4 PE=1 SV=2)

HSP 1 Score: 148.7 bits (374), Expect = 6.5e-35
Identity = 79/177 (44.63%), Postives = 103/177 (58.19%), Query Frame = 0

Query: 1   MKAADVGIEE-----------LEEPESSIVSPRGLIYAKGLPEITYIHIHECDLFSIGIF 60
           +K +DVGIE+           L E   S  SP         P I Y+H+HECD FSIGIF
Sbjct: 37  IKPSDVGIEQDAQLARSRSGPLNERNGSNQSP---------PAIKYLHLHECDSFSIGIF 96

Query: 61  CFPEGGALPLHDHPGMTVMSKVLYGSVHVKAYDWI--KMYGSVGGRTFGLGGKVMDGVWS 120
           C P    +PLH+HPGMTV+SK++YGS+HVK+YDW+  ++              V D   +
Sbjct: 97  CMPPSSMIPLHNHPGMTVLSKLVYGSMHVKSYDWLEPQLTEPEDPSQARPAKLVKDTEMT 156

Query: 121 AGCEARALFPRSGGNIHSFRAMSACAILDVLSPPYSKELGRPSTYFSDLRIPTLPGD 165
           A      L+P+SGGNIH F+A++ CAILD+L+PPYS E  R  TYF   R   LPG+
Sbjct: 157 AQSPVTTLYPKSGGNIHCFKAITHCAILDILAPPYSSEHDRHCTYFRKSRREDLPGE 204

BLAST of Lag0004881 vs. ExPASy Swiss-Prot
Match: Q1G3U6 (Plant cysteine oxidase 3 OS=Arabidopsis thaliana OX=3702 GN=PCO3 PE=1 SV=1)

HSP 1 Score: 145.6 bits (366), Expect = 5.5e-34
Identity = 71/168 (42.26%), Postives = 99/168 (58.93%), Query Frame = 0

Query: 4   ADVGIEELEEPESSIVSPRGLI----YAKGLPEITYIHIHECDLFSIGIFCFPEGGALPL 63
           ADVG+EE+ + +       G+       +    IT++ IHECD F++ IFCFP    +PL
Sbjct: 71  ADVGLEEVSQDDDRGYGVSGVSRFNRVGRWAQPITFLDIHECDTFTMCIFCFPTSSVIPL 130

Query: 64  HDHPGMTVMSKVLYGSVHVKAYDWIKM-------YGSVGGRTFGLGGKVMDGVWSAGCEA 123
           HDHP M V SK+LYGS+HVKAYDW++         G  G     L   V D V +   E 
Sbjct: 131 HDHPEMAVFSKILYGSLHVKAYDWVEPPCIITQDKGVPGSLPARLAKLVSDKVITPQSEI 190

Query: 124 RALFPRSGGNIHSFRAMSACAILDVLSPPYSKELGRPSTYFSDLRIPT 161
            AL+P++GGN+H F A++ CA+LD+LSPPY + +GR  +Y+ D    T
Sbjct: 191 PALYPKTGGNLHCFTALTPCAVLDILSPPYKESVGRSCSYYMDYPFST 238

BLAST of Lag0004881 vs. ExPASy Swiss-Prot
Match: Q9LXT4 (Plant cysteine oxidase 5 OS=Arabidopsis thaliana OX=3702 GN=PCO5 PE=1 SV=1)

HSP 1 Score: 142.5 bits (358), Expect = 4.6e-33
Identity = 74/171 (43.27%), Postives = 102/171 (59.65%), Query Frame = 0

Query: 1   MKAADVGIEELEEPESSIVSPRGLIYAK--GLPEITYIHIHECDLFSIGIFCFPEGGALP 60
           +K +DVG+E+  +   +   P          LP I Y+ +HECD FSIGIFC P G  +P
Sbjct: 37  IKPSDVGLEQEAQLVRNWPGPGNERNGNHHSLPAIKYLQLHECDSFSIGIFCMPPGSIIP 96

Query: 61  LHDHPGMTVMSKVLYGSVHVKAYDWI-----KMYGSVGGRTFGLGGKVMDGVWSAGCEAR 120
           LH+HPGMTV+SK++YGS+HVK+YDW      ++   +  R   L   V D   ++   A 
Sbjct: 97  LHNHPGMTVLSKLVYGSMHVKSYDWAEPDQSELDDPLQARPAKL---VKDIDMTSPSPAT 156

Query: 121 ALFPRSGGNIHSFRAMSACAILDVLSPPYSKELGRPSTYFSDLRIPTLPGD 165
            L+P +GGNIH F+A++ CAI D+LSPPYS   GR   YF    +  LPG+
Sbjct: 157 TLYPTTGGNIHCFKAITHCAIFDILSPPYSSTHGRHCNYFRKSPMLDLPGE 204

BLAST of Lag0004881 vs. ExPASy Swiss-Prot
Match: Q8LGJ5 (Plant cysteine oxidase 2 OS=Arabidopsis thaliana OX=3702 GN=PCO2 PE=1 SV=1)

HSP 1 Score: 135.6 bits (340), Expect = 5.7e-31
Identity = 66/155 (42.58%), Postives = 91/155 (58.71%), Query Frame = 0

Query: 1   MKAADVGIEELEEPESSIVSPRGLIYAKGLPEITYIHIHECDLFSIGIFCFPEGGALPLH 60
           +K  DVG+        S V+ R        P +TY+HI+ C  FSI IFC P  G +PLH
Sbjct: 82  IKPEDVGVNPKMSYFRSTVTGRS-------PLVTYLHIYACHRFSICIFCLPPSGVIPLH 141

Query: 61  DHPGMTVMSKVLYGSVHVKAYDWIKMYGSVGGRTFGLGGKVMDGVWSAGCEARALFPRSG 120
           +HP MTV SK+L+G++H+K+YDW+         T  L    +D  ++A C+   L+P  G
Sbjct: 142 NHPEMTVFSKLLFGTMHIKSYDWVPDSPQPSSDT-RLAKVKVDSDFTAPCDTSILYPADG 201

Query: 121 GNIHSFRAMSACAILDVLSPPYSKELGRPSTYFSD 156
           GN+H F A +ACA+LDV+ PPYS   GR  TY+ D
Sbjct: 202 GNMHCFTAKTACAVLDVIGPPYSDPAGRHCTYYFD 228

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
XP_038886772.15.9e-7081.60plant cysteine oxidase 4-like [Benincasa hispida][more]
KAE8056931.11.7e-5667.27hypothetical protein FH972_013663 [Carpinus fangiana][more]
XP_018858027.11.7e-5665.85plant cysteine oxidase 1-like [Juglans regia] >KAF5456140.1 hypothetical protein... [more]
XP_024174882.14.9e-5663.03plant cysteine oxidase 1 [Rosa chinensis] >PRQ21751.1 putative cysteamine dioxyg... [more]
XP_009373036.16.4e-5664.85PREDICTED: plant cysteine oxidase 1-like isoform X2 [Pyrus x bretschneideri][more]
Match NameE-valueIdentityDescription
Q9LXG98.1e-3843.86Plant cysteine oxidase 1 OS=Arabidopsis thaliana OX=3702 GN=PCO1 PE=1 SV=1[more]
Q9SJI96.5e-3544.63Plant cysteine oxidase 4 OS=Arabidopsis thaliana OX=3702 GN=PCO4 PE=1 SV=2[more]
Q1G3U65.5e-3442.26Plant cysteine oxidase 3 OS=Arabidopsis thaliana OX=3702 GN=PCO3 PE=1 SV=1[more]
Q9LXT44.6e-3343.27Plant cysteine oxidase 5 OS=Arabidopsis thaliana OX=3702 GN=PCO5 PE=1 SV=1[more]
Q8LGJ55.7e-3142.58Plant cysteine oxidase 2 OS=Arabidopsis thaliana OX=3702 GN=PCO2 PE=1 SV=1[more]
InterPro
Analysis Name: InterPro Annotations of Sponge gourd (AG-4) v1
Date Performed: 2022-08-01
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR014710RmlC-like jelly roll foldGENE3D2.60.120.10Jelly Rollscoord: 29..155
e-value: 5.9E-10
score: 40.6
IPR012864Cysteine oxygenase/2-aminoethanethiol dioxygenasePFAMPF07847PCO_ADOcoord: 1..161
e-value: 2.8E-51
score: 173.8
NoneNo IPR availablePANTHERPTHR22966:SF52OS09G0471000 PROTEINcoord: 1..164
NoneNo IPR availablePANTHERPTHR22966UNCHARACTERIZEDcoord: 1..164
NoneNo IPR availableCDDcd20289cupin_ADOcoord: 42..143
e-value: 3.15596E-40
score: 129.978
IPR011051RmlC-like cupin domain superfamilySUPERFAMILY51182RmlC-like cupinscoord: 32..153

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Lag0004881.1Lag0004881.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
molecular_function GO:0017172 cysteine dioxygenase activity
molecular_function GO:0046872 metal ion binding
molecular_function GO:0016702 oxidoreductase activity, acting on single donors with incorporation of molecular oxygen, incorporation of two atoms of oxygen