Lag0004860 (gene) Sponge gourd (AG‐4) v1

BLAST of Lag0004860 vs. NCBI nr
Match: XP_022961975.1 (uncharacterized protein LOC111462585 [Cucurbita moschata])

HSP 1 Score: 562.4 bits (1448), Expect = 2.4e-156
Identity = 266/303 (87.79%), Postives = 279/303 (92.08%), Query Frame = 0

Query: 1   MTRCFFFSITETKNWCHRRSFIKCGLRSTVTDLKDGTTVHCWVPKNPVESKPNLLLIHGI 60
           MTRCFFFSITETKNW HR  F KCGLRST+TDL DGTTVHCWVPKNP+ SKPNLLLIHGI
Sbjct: 1   MTRCFFFSITETKNWYHRHRFTKCGLRSTITDLNDGTTVHCWVPKNPLHSKPNLLLIHGI 60

Query: 61  GVNALWQWGDVIGGLVAHFNVYVPDLIFFGDSFTTRQERTEWFQAQCLMRVMEANSVGKF 120
           G NALWQWGD I  LV HFN+YVPDLIFFGDSF+T  +RTEWFQAQCL+RVMEANSVGK 
Sbjct: 61  GANALWQWGDSIATLVPHFNLYVPDLIFFGDSFSTNPDRTEWFQAQCLIRVMEANSVGKL 120

Query: 121 SLVGLSYGGFVGYSMAVLYPETVEKVVICCAGVCVEEKDFKDGLLRVCDLEEAIAILVPQ 180
           SLVGLSYGGFVGYSMA L P+ VEKVVICCAGVCVEEKDFKDGLLRV DLEEAIAILVPQ
Sbjct: 121 SLVGLSYGGFVGYSMAALCPDMVEKVVICCAGVCVEEKDFKDGLLRVSDLEEAIAILVPQ 180

Query: 181 KSEKLKELVGYSFFRPPRMGLMPSCLLHDFIESMCLDHIEEKRELIRTIPKGRKLSDLPK 240
           K EKLK+LVGYSFFRPP M LMPSCLL+DFIESMCLDHI+EKRELIRTIPKGRKLSDLPK
Sbjct: 181 KPEKLKQLVGYSFFRPPPMRLMPSCLLNDFIESMCLDHIDEKRELIRTIPKGRKLSDLPK 240

Query: 241 IHQRTMILWGEHDQVFPLELGHRLKRHLEDNATLVIIKNAGHAFNSEEPKEFLNHLKSFL 300
           IHQRTMILWGEHD+VFPLELGHRLKRHL DNATLVI+KNAGHAFNSEEPKEFLNHL SFL
Sbjct: 241 IHQRTMILWGEHDEVFPLELGHRLKRHLGDNATLVIVKNAGHAFNSEEPKEFLNHLVSFL 300

Query: 301 VDS 304
           + S
Sbjct: 301 LYS 303

BLAST of Lag0004860 vs. NCBI nr
Match: KAG6598688.1 (Monoacylglycerol lipase ABHD6, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 562.0 bits (1447), Expect = 3.1e-156
Identity = 266/303 (87.79%), Postives = 279/303 (92.08%), Query Frame = 0

Query: 1   MTRCFFFSITETKNWCHRRSFIKCGLRSTVTDLKDGTTVHCWVPKNPVESKPNLLLIHGI 60
           MTRCFFFSITETKNW HR  F KCGLRST+TDL DGTTVHCWVPKNP+ SKPNLLLIHGI
Sbjct: 1   MTRCFFFSITETKNWYHRHRFTKCGLRSTITDLNDGTTVHCWVPKNPLHSKPNLLLIHGI 60

Query: 61  GVNALWQWGDVIGGLVAHFNVYVPDLIFFGDSFTTRQERTEWFQAQCLMRVMEANSVGKF 120
           G NALWQWGD I  LV HFN+YVPDLIFFGDSFTT  +RTEWFQAQCL+RVMEANSVGK 
Sbjct: 61  GANALWQWGDSIATLVPHFNLYVPDLIFFGDSFTTNPDRTEWFQAQCLIRVMEANSVGKL 120

Query: 121 SLVGLSYGGFVGYSMAVLYPETVEKVVICCAGVCVEEKDFKDGLLRVCDLEEAIAILVPQ 180
           SLVGLSYGGFVGYSMA L P+ VEKVV+CCAGVCVEEKDFKDGLLRV DLEEAIAILVPQ
Sbjct: 121 SLVGLSYGGFVGYSMAALCPDMVEKVVVCCAGVCVEEKDFKDGLLRVSDLEEAIAILVPQ 180

Query: 181 KSEKLKELVGYSFFRPPRMGLMPSCLLHDFIESMCLDHIEEKRELIRTIPKGRKLSDLPK 240
           K EKLK+LVGYSFFRPP M LMPSCLL+DFIESMCLDHI+EKRELIRTIPKGRKLSDLPK
Sbjct: 181 KPEKLKQLVGYSFFRPPPMRLMPSCLLNDFIESMCLDHIDEKRELIRTIPKGRKLSDLPK 240

Query: 241 IHQRTMILWGEHDQVFPLELGHRLKRHLEDNATLVIIKNAGHAFNSEEPKEFLNHLKSFL 300
           IHQRTMILWGEHD+VFPLELGHRLKRHL DNATLVIIKNAGHAFNSEEPKEFL+HL SFL
Sbjct: 241 IHQRTMILWGEHDEVFPLELGHRLKRHLGDNATLVIIKNAGHAFNSEEPKEFLDHLVSFL 300

Query: 301 VDS 304
           + S
Sbjct: 301 LYS 303

BLAST of Lag0004860 vs. NCBI nr
Match: XP_023545572.1 (uncharacterized protein LOC111804958 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 560.8 bits (1444), Expect = 7.0e-156
Identity = 266/303 (87.79%), Postives = 278/303 (91.75%), Query Frame = 0

Query: 1   MTRCFFFSITETKNWCHRRSFIKCGLRSTVTDLKDGTTVHCWVPKNPVESKPNLLLIHGI 60
           MTRCFFFSITETKNW HR  F KCGLRST+TDL DGTTVHCWVP NP+ SKPNLLLIHGI
Sbjct: 1   MTRCFFFSITETKNWYHRHRFTKCGLRSTITDLNDGTTVHCWVPNNPLHSKPNLLLIHGI 60

Query: 61  GVNALWQWGDVIGGLVAHFNVYVPDLIFFGDSFTTRQERTEWFQAQCLMRVMEANSVGKF 120
           G NALWQWGD I  LV HFN+YVPDLIFFGDSF+T  +RTEWFQAQCL+RVMEANSVGK 
Sbjct: 61  GANALWQWGDSIATLVPHFNLYVPDLIFFGDSFSTNPDRTEWFQAQCLIRVMEANSVGKL 120

Query: 121 SLVGLSYGGFVGYSMAVLYPETVEKVVICCAGVCVEEKDFKDGLLRVCDLEEAIAILVPQ 180
           SLVGLSYGGFVGYSMA L P+ VEKVVICCAGVCVEEKDFKDGLLRV DLEEAIAILVPQ
Sbjct: 121 SLVGLSYGGFVGYSMAALCPDMVEKVVICCAGVCVEEKDFKDGLLRVSDLEEAIAILVPQ 180

Query: 181 KSEKLKELVGYSFFRPPRMGLMPSCLLHDFIESMCLDHIEEKRELIRTIPKGRKLSDLPK 240
           K EKLK+LVGYSFFRPP M LMPSCLL+DFIESMCLDHI+EKRELIRTIPKGRKLSDLPK
Sbjct: 181 KPEKLKQLVGYSFFRPPPMRLMPSCLLNDFIESMCLDHIDEKRELIRTIPKGRKLSDLPK 240

Query: 241 IHQRTMILWGEHDQVFPLELGHRLKRHLEDNATLVIIKNAGHAFNSEEPKEFLNHLKSFL 300
           IHQRTMILWGEHD+VFPLELGHRLKRHL DNATLVIIKNAGHAFNSEEPKEFLNHL SFL
Sbjct: 241 IHQRTMILWGEHDEVFPLELGHRLKRHLGDNATLVIIKNAGHAFNSEEPKEFLNHLVSFL 300

Query: 301 VDS 304
           + S
Sbjct: 301 LYS 303

BLAST of Lag0004860 vs. NCBI nr
Match: XP_038885546.1 (uncharacterized protein LOC120075885 [Benincasa hispida])

HSP 1 Score: 559.7 bits (1441), Expect = 1.6e-155
Identity = 262/303 (86.47%), Postives = 280/303 (92.41%), Query Frame = 0

Query: 1   MTRCFFFSITETKNWCHRRSFIKCGLRSTVTDLKDGTTVHCWVPKNPVESKPNLLLIHGI 60
           MTRCFFFSITETKNWCHR SFIK GLRSTVTDLKDGTTVHCWVP NP+ +KPNLLLIHGI
Sbjct: 1   MTRCFFFSITETKNWCHRYSFIKSGLRSTVTDLKDGTTVHCWVPNNPIHTKPNLLLIHGI 60

Query: 61  GVNALWQWGDVIGGLVAHFNVYVPDLIFFGDSFTTRQERTEWFQAQCLMRVMEANSVGKF 120
           G NALWQWGD I  LV HFN+Y+PDLIFFGDSFTTR +RTEWFQAQCL+RVMEANSV K 
Sbjct: 61  GANALWQWGDFIPALVPHFNLYIPDLIFFGDSFTTRPDRTEWFQAQCLIRVMEANSVVKL 120

Query: 121 SLVGLSYGGFVGYSMAVLYPETVEKVVICCAGVCVEEKDFKDGLLRVCDLEEAIAILVPQ 180
           SLVGLSYGGFVGYS+A L PE VEKVVICC+G+CVEEKDFKDGLL+V DLEEA AILVPQ
Sbjct: 121 SLVGLSYGGFVGYSIAALRPEMVEKVVICCSGICVEEKDFKDGLLKVSDLEEATAILVPQ 180

Query: 181 KSEKLKELVGYSFFRPPRMGLMPSCLLHDFIESMCLDHIEEKRELIRTIPKGRKLSDLPK 240
           K +KLK+LVGYSFFRPP + LMPSCLL+DFIESMCLDHIEEKRELIRTIP+GRKLSDLPK
Sbjct: 181 KPDKLKQLVGYSFFRPPPLRLMPSCLLNDFIESMCLDHIEEKRELIRTIPRGRKLSDLPK 240

Query: 241 IHQRTMILWGEHDQVFPLELGHRLKRHLEDNATLVIIKNAGHAFNSEEPKEFLNHLKSFL 300
           IHQRTMI+WGEHDQVFPLELGHRLKRHL  NATLV++KNAGHAFNSEEPKEFLNHL SFL
Sbjct: 241 IHQRTMIMWGEHDQVFPLELGHRLKRHLGANATLVVVKNAGHAFNSEEPKEFLNHLISFL 300

Query: 301 VDS 304
           VDS
Sbjct: 301 VDS 303

BLAST of Lag0004860 vs. NCBI nr
Match: KAG7029629.1 (Monoacylglycerol lipase ABHD6 [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 558.1 bits (1437), Expect = 4.5e-155
Identity = 264/300 (88.00%), Postives = 276/300 (92.00%), Query Frame = 0

Query: 4   CFFFSITETKNWCHRRSFIKCGLRSTVTDLKDGTTVHCWVPKNPVESKPNLLLIHGIGVN 63
           CFFFSITETKNW HR  F KCGLRST+TDL DGTTVHCWVPKNP+ SKPNLLLIHGIG N
Sbjct: 510 CFFFSITETKNWYHRHRFTKCGLRSTITDLNDGTTVHCWVPKNPLHSKPNLLLIHGIGAN 569

Query: 64  ALWQWGDVIGGLVAHFNVYVPDLIFFGDSFTTRQERTEWFQAQCLMRVMEANSVGKFSLV 123
           ALWQWGD I  LV HFN+YVPDLIFFGDSFTT  +RTEWFQAQCL+RVMEANSVGK SLV
Sbjct: 570 ALWQWGDSIATLVPHFNLYVPDLIFFGDSFTTNPDRTEWFQAQCLIRVMEANSVGKLSLV 629

Query: 124 GLSYGGFVGYSMAVLYPETVEKVVICCAGVCVEEKDFKDGLLRVCDLEEAIAILVPQKSE 183
           GLSYGGFVGYSMA L P+ VEKVV+CCAGVCVEEKDFKDGLLRV DLEEAIAILVPQK E
Sbjct: 630 GLSYGGFVGYSMAALCPDMVEKVVVCCAGVCVEEKDFKDGLLRVSDLEEAIAILVPQKPE 689

Query: 184 KLKELVGYSFFRPPRMGLMPSCLLHDFIESMCLDHIEEKRELIRTIPKGRKLSDLPKIHQ 243
           KLK+LVGYSFFRPP M LMPSCLL+DFIESMCLDHI+EKRELIRTIPKGRKLSDLPKIHQ
Sbjct: 690 KLKQLVGYSFFRPPPMRLMPSCLLNDFIESMCLDHIDEKRELIRTIPKGRKLSDLPKIHQ 749

Query: 244 RTMILWGEHDQVFPLELGHRLKRHLEDNATLVIIKNAGHAFNSEEPKEFLNHLKSFLVDS 303
           RTMILWGEHD+VFPLELGHRLKRHL DNATLVIIKNAGHAFNSEEPKEFLNHL SFL+ S
Sbjct: 750 RTMILWGEHDEVFPLELGHRLKRHLGDNATLVIIKNAGHAFNSEEPKEFLNHLVSFLLYS 809

BLAST of Lag0004860 vs. ExPASy Swiss-Prot
Match: P23133 (2-hydroxy-6-oxo-2,4-heptadienoate hydrolase OS=Pseudomonas putida (strain ATCC 700007 / DSM 6899 / BCRC 17059 / F1) OX=351746 GN=todF PE=1 SV=2)

HSP 1 Score: 66.6 bits (161), Expect = 5.4e-10
Identity = 70/274 (25.55%), Postives = 114/274 (41.61%), Query Frame = 0

Query: 37  TTVHCWVPKNPVESKPNLLLIHGI--GVNALWQWGDVIGGLVAHFNVYVPDLIFFGDSFT 96
           T +H     NPV      +L+HG   GV A   W  V+  L  H  V  PD++ FG  FT
Sbjct: 19  TNLHDVGAGNPV------VLVHGSGPGVTAWANWRTVMPELSRHRRVIAPDMVGFG--FT 78

Query: 97  TRQERTEW---FQAQCLMRVMEANSVGKFSLVGLSYGGFVGYSMAVLYPETVEKVVICCA 156
            R     +        L  +++A  + +  LVG S+GG +  + A+ +P  V ++V+  A
Sbjct: 79  QRPHGIHYGVESWVAHLAGILDALELDRVDLVGNSFGGALSLAFAIRFPHRVRRLVLMGA 138

Query: 157 -GVCVEEKDFKDGLL----RVCDLEEAIAILVPQKSEKLKELVGYSFFRPPRMGLMPSCL 216
            GV  E  D  D +      V ++ + +      +S    EL    +    R G      
Sbjct: 139 VGVSFELTDGLDAVWGYEPSVPNMRKVMDYFAYDRSLVSDELAELRYKASTRPG------ 198

Query: 217 LHDFIESMCLDHIEEKRELIRTIPKGRKLSDLPKIHQRTMILWGEHDQVFPLELGHRLKR 276
              F E+        ++  +  +    +  D+  I   T+IL G  D+V PLE   RL +
Sbjct: 199 ---FQEAFASMFPAPRQRWVDALASSDQ--DIRDIRHETLILHGRDDRVIPLETSLRLNQ 258

Query: 277 HLEDNATLVIIKNAGHAFNSEEPKEFLNHLKSFL 301
            +E  + L +    GH    E+ + F+  +  FL
Sbjct: 259 LIEP-SQLHVFGRCGHWVQIEQNRGFIRLVNDFL 272

BLAST of Lag0004860 vs. ExPASy Swiss-Prot
Match: O05235 (Uncharacterized hydrolase YugF OS=Bacillus subtilis (strain 168) OX=224308 GN=yugF PE=3 SV=1)

HSP 1 Score: 65.9 bits (159), Expect = 9.3e-10
Identity = 68/281 (24.20%), Postives = 119/281 (42.35%), Query Frame = 0

Query: 35  DGTTVHCWVPKNPVESKPNLLLIHGIGVNALWQWGDVIGGLVAHFNVYVPDLIFFGDSFT 94
           DG  V+    +NP   +  L+ +HG  +++ + +  VI  L   +++   DL  FG S  
Sbjct: 13  DGVNVYYEHYQNP--GRQTLVCVHGF-LSSAFSFRKVIPLLRDKYDIIALDLPPFGQSEK 72

Query: 95  TRQ-ERTEWFQAQCLMRVMEANSVGKFSLVGLSYGGFVGYSMAVLYPETVEKVVICCAGV 154
           +R    T    A+ ++ ++E   V +  LVG S GG +  S A+  PE   KVV+ C+  
Sbjct: 73  SRTFIYTYQNLAKLVIGILEHLQVKQAVLVGHSMGGQISLSAALQKPELFSKVVLLCS-- 132

Query: 155 CVEEKDFKDGLLRVCDLEEAIAILVPQKSEKLKELVGYSFFRPPRMGLMPSCL--LHD-- 214
                    G L+           +P     +K  +        + G+M + L  +HD  
Sbjct: 133 --------SGYLKRSHPTIIFGTHIPYFHLYIKRWLS-------KEGVMKNLLNVVHDKS 192

Query: 215 FIESMCLDH----------IEEKRELIRTIPKGRKLSDLPKIHQRTMILWGEHDQVFPLE 274
            I+   +D            +     IR      +   L K+++  +++WGE D++ P+E
Sbjct: 193 LIDEEMIDGYGRPFQDEQIFKAMTRFIRHREGDLEPEQLKKMNKPALLIWGEEDRIVPME 252

Query: 275 LGHRLKRHLEDNATLVIIKNAGHAFNSEEPKEFLNHLKSFL 301
           +G RL   L  N+ L  +   GH    E P+    H+  F+
Sbjct: 253 IGKRLHADL-PNSVLYSLGQTGHLVPEERPELISEHIADFI 272

BLAST of Lag0004860 vs. ExPASy Swiss-Prot
Match: P91143 (Abhydrolase domain-containing protein abhd-5.1 OS=Caenorhabditis elegans OX=6239 GN=abhd-5.1 PE=3 SV=2)

HSP 1 Score: 62.8 bits (151), Expect = 7.8e-09
Identity = 38/115 (33.04%), Postives = 60/115 (52.17%), Query Frame = 0

Query: 38  TVHCWVPKNPVESKPNLLLIHGIGVNALWQWGDVIGGLVAHFNVYVPDLIFFGDS----F 97
           TV+C    + ++SK  ++LIHG G      WG  I  L    NVY  DL  FG S    F
Sbjct: 58  TVNCENESSELKSKYPIVLIHGFGAGVA-LWGSAIKRLAQFQNVYAIDLPGFGRSSRTKF 117

Query: 98  TTRQERTEWFQAQCLMRVMEANSVGKFSLVGLSYGGFVGYSMAVLYPETVEKVVI 149
           +T  E  E    + + +     ++ K +LVG S+GG++  S A+ YP+ +E +++
Sbjct: 118 STDPETAEKEMIEAIEQWRVKMNLEKMNLVGHSFGGYLSTSYALKYPKRIENLIL 171

BLAST of Lag0004860 vs. ExPASy Swiss-Prot
Match: Q52532 (2-hydroxy-6-oxononadienedioate/2-hydroxy-6-oxononatrienedioate hydrolase OS=Pseudomonas sp. OX=306 GN=mhpC PE=3 SV=1)

HSP 1 Score: 58.9 bits (141), Expect = 1.1e-07
Identity = 67/268 (25.00%), Postives = 105/268 (39.18%), Query Frame = 0

Query: 54  LLLIHGIGVNAL-WQ--WGDVIGGLVAHFNVYVPDLIFFGDSFTTRQERTEWFQAQCLMR 113
           +++ HG G  A  W     +V   + A + V + D   F  S+    +  +   AQ    
Sbjct: 36  VVMFHGSGPGASGWSNFHRNVDAFVDAGYRVLLIDSPGFNKSYPIVTKSRDGAYAQAAKG 95

Query: 114 VMEANSVGKFSLVGLSYGGFVGYSMAVLYPETVEKVVICCAGVCVEEKDFKDGLLRVCDL 173
           VM+   + +  ++G S GG     MAV YPE V K+V+   G                  
Sbjct: 96  VMDKLGIKRAHMIGNSMGGATAMRMAVDYPEMVGKLVMMGGG------------------ 155

Query: 174 EEAIAILVPQKSEKLKELVGYSFFRPPRMGLM----------PSCLLHDFIESMCLDHIE 233
               +   P  +E LK L G   +R P M  +          PS L  + I     +++ 
Sbjct: 156 SVGGSTTTPMPTEGLKLLQG--LYRNPSMENLRKMLDIFVYAPSTLTEELINGR-FENMM 215

Query: 234 EKRELIRTIPKGRKLSD--------LPKIHQRTMILWGEHDQVFPLELGHRLKRHLEDNA 293
            + E +    +  K S         LP +   TMI+WG  D+  PL+LG R+   + D A
Sbjct: 216 RRPEHLTNFVESLKASGGRANYAHLLPTLTMPTMIIWGRDDRFVPLDLGLRMLWGMPD-A 275

Query: 294 TLVIIKNAGHAFNSEEPKEFLNHLKSFL 301
            L +    GH    E   +F   + +FL
Sbjct: 276 ELHVFSKCGHWAQWEHADKFNQLVLNFL 281

BLAST of Lag0004860 vs. ExPASy Swiss-Prot
Match: H2KZ86 (Abhydrolase domain-containing protein abhd-5.2 OS=Caenorhabditis elegans OX=6239 GN=abhd-5.2 PE=1 SV=1)

HSP 1 Score: 56.6 bits (135), Expect = 5.6e-07
Identity = 36/115 (31.30%), Postives = 59/115 (51.30%), Query Frame = 0

Query: 38  TVHCWVPKNPVESKPNLLLIHGIGVNALWQWGDVIGGLVAHFNVYVPDLIFFGDS----F 97
           TV+C   +  V++K  ++LIHG G      WG  I  L     V+  DL  FG S    F
Sbjct: 147 TVNCESEQPIVKAKYPIVLIHGFGAGVA-LWGSAIKRLAQFQTVHAFDLPGFGRSSRPKF 206

Query: 98  TTRQERTEWFQAQCLMRVMEANSVGKFSLVGLSYGGFVGYSMAVLYPETVEKVVI 149
           ++  E  E      + +  +  ++ K +LVG S+GG++  S A+ YP+ VE +++
Sbjct: 207 SSDPETAETEMIDSIEQWRDKMNLEKMNLVGHSFGGYLATSYALKYPKRVENLIL 260

BLAST of Lag0004860 vs. ExPASy TrEMBL
Match: A0A6J1HDR2 (uncharacterized protein LOC111462585 OS=Cucurbita moschata OX=3662 GN=LOC111462585 PE=4 SV=1)

HSP 1 Score: 562.4 bits (1448), Expect = 1.2e-156
Identity = 266/303 (87.79%), Postives = 279/303 (92.08%), Query Frame = 0

Query: 1   MTRCFFFSITETKNWCHRRSFIKCGLRSTVTDLKDGTTVHCWVPKNPVESKPNLLLIHGI 60
           MTRCFFFSITETKNW HR  F KCGLRST+TDL DGTTVHCWVPKNP+ SKPNLLLIHGI
Sbjct: 1   MTRCFFFSITETKNWYHRHRFTKCGLRSTITDLNDGTTVHCWVPKNPLHSKPNLLLIHGI 60

Query: 61  GVNALWQWGDVIGGLVAHFNVYVPDLIFFGDSFTTRQERTEWFQAQCLMRVMEANSVGKF 120
           G NALWQWGD I  LV HFN+YVPDLIFFGDSF+T  +RTEWFQAQCL+RVMEANSVGK 
Sbjct: 61  GANALWQWGDSIATLVPHFNLYVPDLIFFGDSFSTNPDRTEWFQAQCLIRVMEANSVGKL 120

Query: 121 SLVGLSYGGFVGYSMAVLYPETVEKVVICCAGVCVEEKDFKDGLLRVCDLEEAIAILVPQ 180
           SLVGLSYGGFVGYSMA L P+ VEKVVICCAGVCVEEKDFKDGLLRV DLEEAIAILVPQ
Sbjct: 121 SLVGLSYGGFVGYSMAALCPDMVEKVVICCAGVCVEEKDFKDGLLRVSDLEEAIAILVPQ 180

Query: 181 KSEKLKELVGYSFFRPPRMGLMPSCLLHDFIESMCLDHIEEKRELIRTIPKGRKLSDLPK 240
           K EKLK+LVGYSFFRPP M LMPSCLL+DFIESMCLDHI+EKRELIRTIPKGRKLSDLPK
Sbjct: 181 KPEKLKQLVGYSFFRPPPMRLMPSCLLNDFIESMCLDHIDEKRELIRTIPKGRKLSDLPK 240

Query: 241 IHQRTMILWGEHDQVFPLELGHRLKRHLEDNATLVIIKNAGHAFNSEEPKEFLNHLKSFL 300
           IHQRTMILWGEHD+VFPLELGHRLKRHL DNATLVI+KNAGHAFNSEEPKEFLNHL SFL
Sbjct: 241 IHQRTMILWGEHDEVFPLELGHRLKRHLGDNATLVIVKNAGHAFNSEEPKEFLNHLVSFL 300

Query: 301 VDS 304
           + S
Sbjct: 301 LYS 303

BLAST of Lag0004860 vs. ExPASy TrEMBL
Match: A0A6J1KAV5 (uncharacterized protein LOC111492191 OS=Cucurbita maxima OX=3661 GN=LOC111492191 PE=4 SV=1)

HSP 1 Score: 557.8 bits (1436), Expect = 2.9e-155
Identity = 264/303 (87.13%), Postives = 277/303 (91.42%), Query Frame = 0

Query: 1   MTRCFFFSITETKNWCHRRSFIKCGLRSTVTDLKDGTTVHCWVPKNPVESKPNLLLIHGI 60
           MTRCFFFSITETKNW HR  F KCGLRST+TDL DGTTVHCWVPKNP+ SKPNLLLIHGI
Sbjct: 1   MTRCFFFSITETKNWYHRHRFTKCGLRSTITDLNDGTTVHCWVPKNPLHSKPNLLLIHGI 60

Query: 61  GVNALWQWGDVIGGLVAHFNVYVPDLIFFGDSFTTRQERTEWFQAQCLMRVMEANSVGKF 120
           G NALWQWGD I  LV HFN+YVPDLIFFGDS TT  +RTEWFQAQCL+RVMEANSVGK 
Sbjct: 61  GANALWQWGDSIATLVPHFNLYVPDLIFFGDSSTTNPDRTEWFQAQCLIRVMEANSVGKL 120

Query: 121 SLVGLSYGGFVGYSMAVLYPETVEKVVICCAGVCVEEKDFKDGLLRVCDLEEAIAILVPQ 180
           SLVGLSYGGFVGYSMA L P+ VEKVVICCAG+CVEEKDFKDGLLRV DLEEAIAILVPQ
Sbjct: 121 SLVGLSYGGFVGYSMAALCPDMVEKVVICCAGICVEEKDFKDGLLRVSDLEEAIAILVPQ 180

Query: 181 KSEKLKELVGYSFFRPPRMGLMPSCLLHDFIESMCLDHIEEKRELIRTIPKGRKLSDLPK 240
           K EKLK+LVGYSFFRPP M LMPSCLL+DFIESMCLDHI+EKRELIRTIPKGRKLSDLPK
Sbjct: 181 KPEKLKQLVGYSFFRPPPMRLMPSCLLNDFIESMCLDHIDEKRELIRTIPKGRKLSDLPK 240

Query: 241 IHQRTMILWGEHDQVFPLELGHRLKRHLEDNATLVIIKNAGHAFNSEEPKEFLNHLKSFL 300
           IH RTMILWGEHD++FPLELGHRLKRHL DNATLVIIKNAGHAFNSEEPKEFLNHL SFL
Sbjct: 241 IHPRTMILWGEHDEIFPLELGHRLKRHLGDNATLVIIKNAGHAFNSEEPKEFLNHLISFL 300

Query: 301 VDS 304
           + S
Sbjct: 301 LYS 303

BLAST of Lag0004860 vs. ExPASy TrEMBL
Match: A0A5A7VI51 (2-hydroxy-6-oxo-6-(2'-aminophenyl)hexa-2, 4-dienoic acid hydrolase OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold82G004850 PE=4 SV=1)

HSP 1 Score: 544.3 bits (1401), Expect = 3.3e-151
Identity = 252/302 (83.44%), Postives = 278/302 (92.05%), Query Frame = 0

Query: 1   MTRCFFFSITETKNWCHRRSFIKCGLRSTVTDLKDGTTVHCWVPKNPVESKPNLLLIHGI 60
           MT+C FFSITETKNW HR SF K GLRST+TDLKDGTTVHCWVPKNP+ +KPNLLLIHGI
Sbjct: 1   MTKCLFFSITETKNWFHRYSFTKSGLRSTITDLKDGTTVHCWVPKNPIRTKPNLLLIHGI 60

Query: 61  GVNALWQWGDVIGGLVAHFNVYVPDLIFFGDSFTTRQERTEWFQAQCLMRVMEANSVGKF 120
           G NALWQWGD I  L+ +FN+Y+PDLIFFGDSFTT+ +RTEWFQAQCL+RVMEA++VGKF
Sbjct: 61  GANALWQWGDFIPALIPYFNLYIPDLIFFGDSFTTQPDRTEWFQAQCLIRVMEAHTVGKF 120

Query: 121 SLVGLSYGGFVGYSMAVLYPETVEKVVICCAGVCVEEKDFKDGLLRVCDLEEAIAILVPQ 180
           SLVGLSYGGFVGYS+A L PE VE+VVICC+GVCVEEKDFKDGLL+V DLEEA AILVPQ
Sbjct: 121 SLVGLSYGGFVGYSIAALRPEMVERVVICCSGVCVEEKDFKDGLLKVSDLEEATAILVPQ 180

Query: 181 KSEKLKELVGYSFFRPPRMGLMPSCLLHDFIESMCLDHIEEKRELIRTIPKGRKLSDLPK 240
           K EKLK+LVGYSFFRPP + L+PSCLL+DFIESMCLDHIEEKRELIRTIP+GRKLSDLPK
Sbjct: 181 KPEKLKQLVGYSFFRPPPLRLLPSCLLNDFIESMCLDHIEEKRELIRTIPRGRKLSDLPK 240

Query: 241 IHQRTMILWGEHDQVFPLELGHRLKRHLEDNATLVIIKNAGHAFNSEEPKEFLNHLKSFL 300
           I QRT+I+WGEHDQVFP+ELGHRLKRHL DNATLV+IKN GHAFNSEEPKEFLNHL SFL
Sbjct: 241 IQQRTLIMWGEHDQVFPVELGHRLKRHLGDNATLVVIKNTGHAFNSEEPKEFLNHLISFL 300

Query: 301 VD 303
           VD
Sbjct: 301 VD 302

BLAST of Lag0004860 vs. ExPASy TrEMBL
Match: A0A0A0LLN7 (AB hydrolase-1 domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_2G368940 PE=4 SV=1)

HSP 1 Score: 542.3 bits (1396), Expect = 1.2e-150
Identity = 252/302 (83.44%), Postives = 277/302 (91.72%), Query Frame = 0

Query: 1   MTRCFFFSITETKNWCHRRSFIKCGLRSTVTDLKDGTTVHCWVPKNPVESKPNLLLIHGI 60
           MT+CFFFSI ETKNW HR SF K GLRST+TDLKDGTTVHCWVPKNP+ +KPNLLLIHGI
Sbjct: 1   MTKCFFFSIVETKNWFHRYSFTKSGLRSTITDLKDGTTVHCWVPKNPIHTKPNLLLIHGI 60

Query: 61  GVNALWQWGDVIGGLVAHFNVYVPDLIFFGDSFTTRQERTEWFQAQCLMRVMEANSVGKF 120
           G NALWQWGD I  L+ +FN+Y+PDLIFFGDSFTT+ +RTEWFQAQCL+RVMEAN+VGKF
Sbjct: 61  GANALWQWGDFIPALIPYFNLYIPDLIFFGDSFTTQPDRTEWFQAQCLIRVMEANTVGKF 120

Query: 121 SLVGLSYGGFVGYSMAVLYPETVEKVVICCAGVCVEEKDFKDGLLRVCDLEEAIAILVPQ 180
           SLVGLSYGGFVGYS+A L PE VE+VVICC+GVCVEEKDFKDGLL+V  LE+A AILVPQ
Sbjct: 121 SLVGLSYGGFVGYSIAALRPEMVERVVICCSGVCVEEKDFKDGLLKVSALEDATAILVPQ 180

Query: 181 KSEKLKELVGYSFFRPPRMGLMPSCLLHDFIESMCLDHIEEKRELIRTIPKGRKLSDLPK 240
           K EKLK+LVGYSFFRPP + L+PSCLL+DFIESMCLDHIEEKRELIRTIP+GRKLSDLPK
Sbjct: 181 KPEKLKQLVGYSFFRPPPLRLIPSCLLNDFIESMCLDHIEEKRELIRTIPRGRKLSDLPK 240

Query: 241 IHQRTMILWGEHDQVFPLELGHRLKRHLEDNATLVIIKNAGHAFNSEEPKEFLNHLKSFL 300
           I QRTMI+WGEHDQVFPLELGHRLKRHL DNATLV+IKN GHAFNSEEPKEFL+HL SFL
Sbjct: 241 IQQRTMIMWGEHDQVFPLELGHRLKRHLGDNATLVVIKNTGHAFNSEEPKEFLSHLISFL 300

Query: 301 VD 303
           VD
Sbjct: 301 VD 302

BLAST of Lag0004860 vs. ExPASy TrEMBL
Match: A0A1S3BC20 (LOW QUALITY PROTEIN: 2-hydroxy-6-oxo-6-(2'-aminophenyl)hexa-2,4-dienoic acid hydrolase OS=Cucumis melo OX=3656 GN=LOC103488063 PE=4 SV=1)

HSP 1 Score: 541.6 bits (1394), Expect = 2.1e-150
Identity = 251/302 (83.11%), Postives = 277/302 (91.72%), Query Frame = 0

Query: 1   MTRCFFFSITETKNWCHRRSFIKCGLRSTVTDLKDGTTVHCWVPKNPVESKPNLLLIHGI 60
           MT+C FFSITETKNW HR SF K GLRST+TDLKDGTTVHCWVPKNP+ +KPNLLLIHGI
Sbjct: 1   MTKCLFFSITETKNWFHRYSFTKSGLRSTITDLKDGTTVHCWVPKNPIRTKPNLLLIHGI 60

Query: 61  GVNALWQWGDVIGGLVAHFNVYVPDLIFFGDSFTTRQERTEWFQAQCLMRVMEANSVGKF 120
           G NALWQWGD I  L+ +FN+Y+PDLIFFGDSFTT+ +RTEWFQAQCL+RVMEA++VGKF
Sbjct: 61  GANALWQWGDFIPALIPYFNLYIPDLIFFGDSFTTQPDRTEWFQAQCLIRVMEAHTVGKF 120

Query: 121 SLVGLSYGGFVGYSMAVLYPETVEKVVICCAGVCVEEKDFKDGLLRVCDLEEAIAILVPQ 180
           SLVGLSYGGFVGYS+A L PE VE+VVICC+GVCVEEKDFKDGLL+V DLEEA AILVPQ
Sbjct: 121 SLVGLSYGGFVGYSIAALRPEMVERVVICCSGVCVEEKDFKDGLLKVSDLEEATAILVPQ 180

Query: 181 KSEKLKELVGYSFFRPPRMGLMPSCLLHDFIESMCLDHIEEKRELIRTIPKGRKLSDLPK 240
           K EKLK+LVGYSF RPP + L+PSCLL+DFIESMCLDHIEEKRELIRTIP+GRKLSDLPK
Sbjct: 181 KPEKLKQLVGYSFXRPPPLRLLPSCLLNDFIESMCLDHIEEKRELIRTIPRGRKLSDLPK 240

Query: 241 IHQRTMILWGEHDQVFPLELGHRLKRHLEDNATLVIIKNAGHAFNSEEPKEFLNHLKSFL 300
           I QRT+I+WGEHDQVFP+ELGHRLKRHL DNATLV+IKN GHAFNSEEPKEFLNHL SFL
Sbjct: 241 IQQRTLIMWGEHDQVFPVELGHRLKRHLGDNATLVVIKNTGHAFNSEEPKEFLNHLISFL 300

Query: 301 VD 303
           VD
Sbjct: 301 VD 302

BLAST of Lag0004860 vs. TAIR 10
Match: AT5G09430.1 (alpha/beta-Hydrolases superfamily protein )

HSP 1 Score: 379.8 bits (974), Expect = 2.1e-105
Identity = 177/308 (57.47%), Postives = 235/308 (76.30%), Query Frame = 0

Query: 1   MTRCFFFSITETKNWCHRRSFIKCGLRSTVTDLKDG-----TTVHCWVPKNPVESKPNLL 60
           + RC  FS T +++W  R+SF   GLRS  TDL  G     T +HCW+PK+P  SKPNLL
Sbjct: 7   LLRC--FSFTASRDWLFRQSFANAGLRSVTTDLSHGNSIASTAMHCWIPKSPNRSKPNLL 66

Query: 61  LIHGIGVNALWQWGDVIGGLVAHFNVYVPDLIFFGDSFTTRQERTEWFQAQCLMRVMEAN 120
           L+HG G NA+WQ+G+ +      FNVYVPDL+FFG S T+   RTE FQA+CLMR+MEA+
Sbjct: 67  LLHGFGANAMWQYGEHLRAFTGRFNVYVPDLLFFGLSSTSEPNRTESFQARCLMRLMEAH 126

Query: 121 SVGKFSLVGLSYGGFVGYSMAVLYPETVEKVVICCAGVCVEEKDFKDGLLRVCDLEEAIA 180
            V + ++VG+SYGGFVGYS+A  +PE VEK+V+CCAGVC+EEKD +DGL +V +LEEA  
Sbjct: 127 GVQRMNIVGISYGGFVGYSLAAQFPENVEKLVLCCAGVCLEEKDMEDGLFKVPNLEEATG 186

Query: 181 ILVPQKSEKLKELVGYSFFRPPRMGLMPSCLLHDFIESMCLDHIEEKRELIRTIPKGRKL 240
           IL+PQ  EKLKEL+ +SF +P +   +PS  L DFI+ MC + +EEKR+LI++I K R+L
Sbjct: 187 ILIPQTPEKLKELIRFSFVKPIKG--VPSFFLWDFIDVMCTEFVEEKRDLIKSILKDRRL 246

Query: 241 SDLPKIHQRTMILWGEHDQVFPLELGHRLKRHLEDNATLVIIKNAGHAFNSEEPKEFLNH 300
           SDLP+I Q+++I+WGE DQ+FPLELG+RLKRH+ ++A +V+IK AGHA N E+ KEF+ H
Sbjct: 247 SDLPRIKQKSLIIWGEEDQIFPLELGYRLKRHIGESAEIVVIKKAGHAVNLEKSKEFVKH 306

Query: 301 LKSFLVDS 304
           LKSFL+DS
Sbjct: 307 LKSFLIDS 310

BLAST of Lag0004860 vs. TAIR 10
Match: AT1G78210.1 (alpha/beta-Hydrolases superfamily protein )

HSP 1 Score: 371.7 bits (953), Expect = 5.6e-103
Identity = 181/306 (59.15%), Postives = 230/306 (75.16%), Query Frame = 0

Query: 1   MTRCFFFSITETKNWCHRRSFIKCGLRSTVTDLKDGTTVHCWVPKNPVES--KPNLLLIH 60
           MT C  FS++E     ++  F + GLR    DLKDGT V+ WV K   ES  KPNLLLIH
Sbjct: 1   MTGC--FSLSEALERTYKSGFKRSGLRPVTIDLKDGTVVNFWVSKTKPESKPKPNLLLIH 60

Query: 61  GIGVNALWQWGDVIGGLVAHFNVYVPDLIFFGDSFTTRQERTEWFQAQCLMRVMEANSVG 120
           G+G  A+WQW DV   L  +FN+Y+PDL+FFG S TTR ER++ FQAQ LMR +EA SV 
Sbjct: 61  GLGATAIWQWYDVARRLSRYFNLYIPDLVFFGGSSTTRPERSDIFQAQTLMRALEAQSVK 120

Query: 121 KFSLVGLSYGGFVGYSMAVLYPETVEKVVICCAGVCVEEKDFKDGLLRVCDLEEAIAILV 180
           KFSLVGLSYGGFVGY MA +Y + VEKVVICCA VCVEEKD K G+ +V DL+EA  ILV
Sbjct: 121 KFSLVGLSYGGFVGYRMASMYADAVEKVVICCAAVCVEEKDMKAGVFKVSDLDEASKILV 180

Query: 181 PQKSEKLKELVGYSFFRPPRMGLMPSCLLHDFIE-SMCLDHIEEKRELIRTIPKGRKLSD 240
           P+  +KL+EL+GY F++P    L+P+CLLHDFIE ++  D++EEKRELI+ IPK R +S+
Sbjct: 181 PESVKKLRELMGYIFYKPALARLVPTCLLHDFIEHALTRDNMEEKRELIKAIPKDRIISE 240

Query: 241 LPKIHQRTMILWGEHDQVFPLELGHRLKRHLEDNATLVIIKNAGHAFNSEEPKEFLNHLK 300
           +PK+ Q T+I+WGEHDQVFPLE+G RL++H+ DN  LVIIK  GH FN E+PK+F+  LK
Sbjct: 241 IPKLKQPTLIIWGEHDQVFPLEMGKRLEKHVGDNGKLVIIKRTGHIFNFEKPKKFIKLLK 300

Query: 301 SFLVDS 304
           SFL+++
Sbjct: 301 SFLLET 304

BLAST of Lag0004860 vs. TAIR 10
Match: AT4G39955.1 (alpha/beta-Hydrolases superfamily protein )

HSP 1 Score: 340.5 bits (872), Expect = 1.4e-93
Identity = 155/295 (52.54%), Postives = 211/295 (71.53%), Query Frame = 0

Query: 7   FSITETKNWCHRRSFIKCGLRSTVTDLKDGTTVHCWVPKNPVESKPNLLLIHGIGVNALW 66
           FS   ++N C++ SF + GLRS+ +DL DGT  HCW+P   + +KP LLL+HGIG NA+W
Sbjct: 5   FSYVSSRNKCYQYSFSRAGLRSSTSDLGDGTVFHCWIPLTHIHTKPTLLLLHGIGANAMW 64

Query: 67  QWGDVIGGLVAHFNVYVPDLIFFGDSFTTRQERTEWFQAQCLMRVMEANSVGKFSLVGLS 126
           QW   I   +  FNVYVPDLIFFGDS+TTR +R+E FQA C+M+ M+A  V   ++ GLS
Sbjct: 65  QWDRFIDRFIPRFNVYVPDLIFFGDSYTTRPDRSESFQATCVMKAMDAYGVRTMTVAGLS 124

Query: 127 YGGFVGYSMAVLYPETVEKVVICCAGVCVEEKDFKDGLLRVCDLEEAIAILVPQKSEKLK 186
           YGGFV YS+A  + E V++VV+ CAGV +EEKD +DG+ +V   EEA A+L PQ    L+
Sbjct: 125 YGGFVAYSLAAQFKERVDRVVLICAGVALEEKDSEDGMFKVKSPEEAAAVLFPQSPSMLR 184

Query: 187 ELVGYSFFRPPRMGLMPSCLLHDFIESMCLDHIEEKRELIRTIPKGRKLSDLPKIHQRTM 246
            L+  SF++PP    +PSC   D+I  MC D+++E++EL+  + KGR+ ++LPKI Q T+
Sbjct: 185 RLLQLSFYKPPIW--IPSCFAMDYIHVMCKDYLQERKELVEALHKGRRFANLPKITQPTL 244

Query: 247 ILWGEHDQVFPLELGHRLKRHL-EDNATLVIIKNAGHAFNSEEPKEFLNHLKSFL 301
           ++WGE DQVFP+EL HRLKR+L ED A LV++K  GHA N E+PKE   H+KSFL
Sbjct: 245 MIWGEEDQVFPVELAHRLKRYLGEDRAQLVLLKKTGHAINEEKPKEMYKHMKSFL 297

BLAST of Lag0004860 vs. TAIR 10
Match: AT5G21950.1 (alpha/beta-Hydrolases superfamily protein )

HSP 1 Score: 202.2 bits (513), Expect = 5.9e-52
Identity = 110/301 (36.54%), Postives = 164/301 (54.49%), Query Frame = 0

Query: 6   FFSITETKNWCHRRSFIKCGLRSTVTDLKDGTTVHCWVPKNPVES----KPNLLLIHGIG 65
           F S+        RR F   GL      +   TT+  W P     S    KP+LLL+HG G
Sbjct: 5   FLSVARFVEALLRRRFSSAGLSLQTLSIDSETTIQFWGPPPSSSSENTQKPSLLLLHGFG 64

Query: 66  VNALWQWGDVIGGLVAHFNVYVPDLIFFGDSFTTRQERTEWFQAQCLMRVMEANSVGKFS 125
            +A+WQW   +  L   F +YVPDL+FFG S ++ + R+E FQA C+ ++ME   V +FS
Sbjct: 65  PSAVWQWSHQVKPLSHFFRLYVPDLVFFGGSSSSGENRSEMFQALCMGKLMEKLEVERFS 124

Query: 126 LVGLSYGGFVGYSMAVLYPETVEKVVICCAGVCVEEKDFKDGLLRVCDLEEAIAILVPQK 185
           +VG SYGGFV Y+MA ++PE VEKVV+  +GV +   D  +  +          +++P  
Sbjct: 125 VVGTSYGGFVAYNMAKMFPEKVEKVVLASSGVNLRRSD-NEAFIARAKCHRIKEVMLPAS 184

Query: 186 SEKLKELVGYSFFRPPRMGLMPSCLLHDFIESMCLDHIEEKRELIRTIPKGR-KLSDLPK 245
           +  L+   G       R+  +P  +L+DF + M  +  EEK EL+  +  G+   +++  
Sbjct: 185 ATDLRRFSG--MVSSKRLDYVPDFVLNDFCQKMYSEKREEKAELLEGLSIGKDDKTNVSP 244

Query: 246 IHQRTMILWGEHDQVFPLELGHRLKRHLEDNATLVIIKNAGHAFNSEEPKEFLNHLKSFL 302
           I Q  M++WGE DQVFPL++ H LK  L   ATL +I+   H   +E+ KEF   + SFL
Sbjct: 245 IQQDVMLIWGEQDQVFPLKMAHDLKEMLGTKATLKVIQKTSHIPQTEKSKEFNGFVMSFL 302

BLAST of Lag0004860 vs. TAIR 10
Match: AT4G33180.1 (alpha/beta-Hydrolases superfamily protein )

HSP 1 Score: 181.4 bits (459), Expect = 1.1e-45
Identity = 103/293 (35.15%), Postives = 164/293 (55.97%), Query Frame = 0

Query: 18  RRSFIKCGLRSTVTDLKDGTTVHCWVP----KNPVESKPNLLLIHGIGVNALWQWGDVIG 77
           RR     GL S    +   TT+H W P        + +P +LL+HG G +++WQW   + 
Sbjct: 18  RRCLRAAGLTSQTLSIDSETTIHFWGPPPLDHRSDDDRPVMLLLHGFGPSSMWQWRRQMQ 77

Query: 78  GL-VAHFNVYVPDLIFFGDSFTTRQERTEWFQAQCLMRVMEANSVGKFSLVGLSYGGFVG 137
               + F VY PDL+FFGDS ++   RTE FQA+C+ ++M    +GK+++ G SYGGFV 
Sbjct: 78  AFSPSAFRVYSPDLVFFGDSTSSSTNRTEVFQAECMAKLMAKIGIGKYNVAGTSYGGFVA 137

Query: 138 YSMAVLYPETVEKVVICCAGVCVEEKDFKDGLLRVCDLEEAIAILVPQKSEKLKELVGY- 197
           Y MA ++PE VEKVVI  +G+ + + D  + LL+  + E    +++P  + + + L+   
Sbjct: 138 YHMAKMWPEKVEKVVIASSGINMRKCD-GESLLQRSNCECIEKVMLPSTATEFRTLMALA 197

Query: 198 SFFRPPRMGLMPSCLLHDFIESMCLDHIEEKRELIRTIPKGRKLS-DLPKIHQRTMILWG 257
           S +R  RM   P  L +D I ++   + +EK EL++ +  GR  + ++  + Q  +I+WG
Sbjct: 198 SSWRLVRM--FPDALWNDVINNLYKKNRKEKIELLKGVTFGRSENLNIDSLSQEVLIVWG 257

Query: 258 EHDQVFPLELGHRLKRHLEDNATLVIIKNAGHAFNSEEPKEFLNHLKSFLVDS 304
           + DQ+FP+++ + LK  L D   L II N  H    E  +EF N +  FL  S
Sbjct: 258 DKDQIFPVKMAYELKEILGDKTKLEIIDNTSHVPQIECAQEFNNIVLRFLKGS 307