Lag0004853 (gene) Sponge gourd (AG‐4) v1

BLAST of Lag0004853 vs. NCBI nr
Match: KAG6585288.1 (F-box protein, partial [Cucurbita argyrosperma subsp. sororia] >KAG7020208.1 F-box protein, partial [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 502.7 bits (1293), Expect = 2.5e-138
Identity = 277/346 (80.06%), Postives = 302/346 (87.28%), Query Frame = 0

Query: 1   MAERQDSSSIHHLDDAFDCLPDSLILLIFNAVSDVKTLIRCRAVSKRFNSLVPHSDSLSL 60
           MAE QDSS I H+DDAFD LPDS+ILLIFN+VSDVKTLIRCRAVSKRFNSLVPHSDSL L
Sbjct: 1   MAEGQDSSLI-HIDDAFDSLPDSIILLIFNSVSDVKTLIRCRAVSKRFNSLVPHSDSLFL 60

Query: 61  KVDCVIS--SDSDENSFLFAFFKSVVKSFFDLISPILVQSESQNSPAQILRQFARIHRLE 120
           KVDCVIS  SDSDENSFL+ FFKSVVKSFFDLISPILVQSESQNSPAQILRQF RI RLE
Sbjct: 61  KVDCVISSDSDSDENSFLYGFFKSVVKSFFDLISPILVQSESQNSPAQILRQFIRIQRLE 120

Query: 121 IEFPTGDPKADRVGGVKWRAEFGITMRSCVILIFRGIR-DLDDRAAMAEDYFDFIGGLKS 180
           IEFPTGD K +R+GGVKWRAEFGIT+RSCVIL+FRGIR +  D  A+A D FDFI  LKS
Sbjct: 121 IEFPTGDLKVERIGGVKWRAEFGITLRSCVILLFRGIRYESYDETAVA-DGFDFIEELKS 180

Query: 181 KVFWTISTLITASARHHVLTEVVEEHLEMESLVLRDRGGEGVVVMEKDGLEELRKSTERV 240
           KVF TISTLITASARHHVL EV+EEHLE+ESLVLRDR GEGVV M+KDGL+E RKS +R 
Sbjct: 181 KVFLTISTLITASARHHVLAEVIEEHLEVESLVLRDREGEGVVEMDKDGLDEWRKSRDRS 240

Query: 241 ---GGGLAAAVEGEEWQRTRTRVPSTTVRMRHLPRVELRRGLWMEDATLVVVRPTGDASK 300
              GGG+AA  EG EW+RTRTRVPSTTVRMRHLPRVELRRG+WMEDATLVVVRPT +ASK
Sbjct: 241 GEDGGGVAA--EGGEWRRTRTRVPSTTVRMRHLPRVELRRGMWMEDATLVVVRPTTNASK 300

Query: 301 DGAADLEREDADVASRAFEEGENVYREAVKALLKKGRRYQLEMNSF 341
           D   D+E+E+ +VA R F EG++VY EAV ALLKKGRRYQLEMNSF
Sbjct: 301 DKTDDMEKENDEVAFRTF-EGDDVYGEAVGALLKKGRRYQLEMNSF 341

BLAST of Lag0004853 vs. NCBI nr
Match: XP_023002829.1 (F-box protein At1g78100-like [Cucurbita maxima])

HSP 1 Score: 501.9 bits (1291), Expect = 4.3e-138
Identity = 276/346 (79.77%), Postives = 302/346 (87.28%), Query Frame = 0

Query: 1   MAERQDSSSIHHLDDAFDCLPDSLILLIFNAVSDVKTLIRCRAVSKRFNSLVPHSDSLSL 60
           MAE QDSS I H+DDAFD LPDS+IL+IFN+VSDVKTLIRCRAVSKRFNSLVPHSDSL L
Sbjct: 1   MAEGQDSSLI-HIDDAFDSLPDSIILMIFNSVSDVKTLIRCRAVSKRFNSLVPHSDSLFL 60

Query: 61  KVDCVIS--SDSDENSFLFAFFKSVVKSFFDLISPILVQSESQNSPAQILRQFARIHRLE 120
           KVDCVIS  SDSDENSFL+ FFKSVVKSFFDLISPILVQSESQNSPAQILRQF RI RLE
Sbjct: 61  KVDCVISSDSDSDENSFLYGFFKSVVKSFFDLISPILVQSESQNSPAQILRQFIRIQRLE 120

Query: 121 IEFPTGDPKADRVGGVKWRAEFGITMRSCVILIFRGIR-DLDDRAAMAEDYFDFIGGLKS 180
           IEFPTGD K +R+GGVKWRAEFGIT+RSCVIL+FRGIR +  D  A+A D FDFI  LKS
Sbjct: 121 IEFPTGDLKVERIGGVKWRAEFGITLRSCVILLFRGIRYESYDETAVA-DGFDFIEELKS 180

Query: 181 KVFWTISTLITASARHHVLTEVVEEHLEMESLVLRDRGGEGVVVMEKDGLEELRKSTERV 240
           KVF TISTLITASARHHVL E++EEHLEMESLVLRDR GEGVV M+KDGL+E RKS ER 
Sbjct: 181 KVFLTISTLITASARHHVLAELIEEHLEMESLVLRDREGEGVVEMDKDGLDEWRKSRERS 240

Query: 241 ---GGGLAAAVEGEEWQRTRTRVPSTTVRMRHLPRVELRRGLWMEDATLVVVRPTGDASK 300
              GGG+AA  EG EW+RTRTRVPSTTVRMRHLPRVELRRG+W+EDATLVVVRPT +ASK
Sbjct: 241 GEDGGGVAA--EGGEWRRTRTRVPSTTVRMRHLPRVELRRGMWIEDATLVVVRPTTNASK 300

Query: 301 DGAADLEREDADVASRAFEEGENVYREAVKALLKKGRRYQLEMNSF 341
           D   D+E+E+ +VA R F EG++VY EAV ALLKKGRRYQLEMNSF
Sbjct: 301 DKTDDMEKENDEVALRTF-EGDDVYGEAVGALLKKGRRYQLEMNSF 341

BLAST of Lag0004853 vs. NCBI nr
Match: XP_023537288.1 (F-box protein At1g22220-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 501.1 bits (1289), Expect = 7.4e-138
Identity = 277/346 (80.06%), Postives = 302/346 (87.28%), Query Frame = 0

Query: 1   MAERQDSSSIHHLDDAFDCLPDSLILLIFNAVSDVKTLIRCRAVSKRFNSLVPHSDSLSL 60
           MAE QDSS I H+DDAFD LPDS+ILLIFN+VSDVKTLIRCRAVSKRFNSLVPHSDSL L
Sbjct: 1   MAEGQDSSLI-HIDDAFDSLPDSIILLIFNSVSDVKTLIRCRAVSKRFNSLVPHSDSLFL 60

Query: 61  KVDCVIS--SDSDENSFLFAFFKSVVKSFFDLISPILVQSESQNSPAQILRQFARIHRLE 120
           KVDCVIS  SDSDENSFL+ FFKSVVKSFFDLISPILVQSESQNSPAQILRQF RI RLE
Sbjct: 61  KVDCVISSDSDSDENSFLYGFFKSVVKSFFDLISPILVQSESQNSPAQILRQFIRIQRLE 120

Query: 121 IEFPTGDPKADRVGGVKWRAEFGITMRSCVILIFRGIR-DLDDRAAMAEDYFDFIGGLKS 180
           IEFPTGD K +R+GGVKWRAEFGIT+RSCVIL+FRGIR +  D  A+A D FDFI  LKS
Sbjct: 121 IEFPTGDLKVERIGGVKWRAEFGITLRSCVILLFRGIRYESYDETAVA-DGFDFIEELKS 180

Query: 181 KVFWTISTLITASARHHVLTEVVEEHLEMESLVLRDRGGEGVVVMEKDGLEELRKSTERV 240
           KVF TISTLITASARH+VL EV+EEHLE+ESLVLRDR GEGVV M+KDGL+E RKS ER 
Sbjct: 181 KVFLTISTLITASARHNVLAEVIEEHLEVESLVLRDREGEGVVEMDKDGLDEWRKSRERS 240

Query: 241 ---GGGLAAAVEGEEWQRTRTRVPSTTVRMRHLPRVELRRGLWMEDATLVVVRPTGDASK 300
              GGG+AA  EG EW+RTRTRVPSTTVRMRHLPRVELRRG+WMEDATLVVVRPT +ASK
Sbjct: 241 GEDGGGVAA--EGGEWRRTRTRVPSTTVRMRHLPRVELRRGMWMEDATLVVVRPTTNASK 300

Query: 301 DGAADLEREDADVASRAFEEGENVYREAVKALLKKGRRYQLEMNSF 341
           D   D+E+E+ +VA R F EG++VY EAV ALLKKGRRYQLEMNSF
Sbjct: 301 DKTDDMEKENDEVALRTF-EGDDVYGEAVGALLKKGRRYQLEMNSF 341

BLAST of Lag0004853 vs. NCBI nr
Match: XP_022951596.1 (F-box protein At1g22220-like [Cucurbita moschata])

HSP 1 Score: 500.0 bits (1286), Expect = 1.6e-137
Identity = 274/344 (79.65%), Postives = 299/344 (86.92%), Query Frame = 0

Query: 1   MAERQDSSSIHHLDDAFDCLPDSLILLIFNAVSDVKTLIRCRAVSKRFNSLVPHSDSLSL 60
           MAE QDSS I H+DDAFD LPDS+ILLIFN+VSDVKTLIRCRAVSKRFNSLVPHSDSL L
Sbjct: 1   MAEGQDSSLI-HIDDAFDSLPDSIILLIFNSVSDVKTLIRCRAVSKRFNSLVPHSDSLFL 60

Query: 61  KVDCVIS--SDSDENSFLFAFFKSVVKSFFDLISPILVQSESQNSPAQILRQFARIHRLE 120
           KVDCVIS  SDSDENSFL+ FFKSVVKSFFDLISPILVQSESQNSPAQILRQF RI RLE
Sbjct: 61  KVDCVISSDSDSDENSFLYGFFKSVVKSFFDLISPILVQSESQNSPAQILRQFIRIQRLE 120

Query: 121 IEFPTGDPKADRVGGVKWRAEFGITMRSCVILIFRGIR-DLDDRAAMAEDYFDFIGGLKS 180
           IEFPTGD K +R+GGVKWRAEFGIT+RSCVIL+FRGIR +  D  A+A D FDFI  LKS
Sbjct: 121 IEFPTGDLKVERIGGVKWRAEFGITLRSCVILLFRGIRYESYDETAVA-DGFDFIEELKS 180

Query: 181 KVFWTISTLITASARHHVLTEVVEEHLEMESLVLRDRGGEGVVVMEKDGLEELRKSTERV 240
           KVF TISTLITASARHHVL EV+EEHLE++SLVLRDR GEGVV M+KDGL+E RKS +R 
Sbjct: 181 KVFLTISTLITASARHHVLAEVIEEHLEVDSLVLRDREGEGVVEMDKDGLDEWRKSRDRS 240

Query: 241 G-GGLAAAVEGEEWQRTRTRVPSTTVRMRHLPRVELRRGLWMEDATLVVVRPTGDASKDG 300
           G  G   A EG EW+RTRTRVPSTTVRMRHLPRVELRRG+WMEDATLVVVRPT +ASKD 
Sbjct: 241 GEDGGGVAGEGGEWRRTRTRVPSTTVRMRHLPRVELRRGMWMEDATLVVVRPTTNASKDK 300

Query: 301 AADLEREDADVASRAFEEGENVYREAVKALLKKGRRYQLEMNSF 341
             D+E+E+ +VA R F EG++VY EAV ALLKKGRRYQLEMNSF
Sbjct: 301 TDDMEKENDEVALRTF-EGDDVYGEAVGALLKKGRRYQLEMNSF 341

BLAST of Lag0004853 vs. NCBI nr
Match: XP_038886087.1 (F-box protein At1g22220 [Benincasa hispida])

HSP 1 Score: 477.2 bits (1227), Expect = 1.1e-130
Identity = 261/340 (76.76%), Postives = 289/340 (85.00%), Query Frame = 0

Query: 3   ERQDSSSIHHLDDAFDCLPDSLILLIFNAVSDVKTLIRCRAVSKRFNSLVPHSDSLSLKV 62
           + QD+SSIH+ DD FD LPDSLILLIFN+V DVKTLIRCRAVSKRFNSLVPHSDSLS+KV
Sbjct: 5   QEQDASSIHNNDDGFDSLPDSLILLIFNSVCDVKTLIRCRAVSKRFNSLVPHSDSLSVKV 64

Query: 63  DCVIS--SDSDENSFLFAFFKSVVKSFFDLISPILVQSESQNSPAQILRQFARIHRLEIE 122
           DCVIS  SDSD++SFL  FFKSVVKSFF LIS +LVQSESQNSPAQILRQF +I RL+IE
Sbjct: 65  DCVISSDSDSDQDSFLLTFFKSVVKSFFHLISTVLVQSESQNSPAQILRQFRQIQRLQIE 124

Query: 123 FPTGDPKADRVGGVKWRAEFGITMRSCVILIFRGIRDLDDRAAMAEDYFDFIGGLKSKVF 182
           FPTGD K +RV  VKW+AEFG  ++SCVILIFRGIR   +  A  +  FDFIGGLKSKVF
Sbjct: 125 FPTGDLKVERV--VKWKAEFGDALKSCVILIFRGIRS-GEIVADEDVDFDFIGGLKSKVF 184

Query: 183 WTISTLITASARHHVLTEVVEEHLEMESLVLRDRGGEGVVVMEKDGLEELRKSTERVGGG 242
            TISTLITASARHHVL EVV EHLEMESLVLRDRGGEGVVVMEK+GLEELR+S    GG 
Sbjct: 185 MTISTLITASARHHVLAEVVVEHLEMESLVLRDRGGEGVVVMEKNGLEELRRSRVSDGGE 244

Query: 243 LAAAVEGEEWQRTRTRVPSTTVRMRHLPRVELRRGLWMEDATLVVVRPTGDASKDGAADL 302
           +A      EW RTRTRVPSTTVRMRH  RVELR G+WMEDATLVVVRPTG+ASK GAAD+
Sbjct: 245 VA------EWTRTRTRVPSTTVRMRHRGRVELRGGMWMEDATLVVVRPTGNASKSGAADV 304

Query: 303 EREDADVASRAFEEGENVYREAVKALLKKGRRYQLEMNSF 341
           E++DADVA RAF EG++ YREAV ALL+KG+RYQLEMNSF
Sbjct: 305 EKDDADVAMRAF-EGDDAYREAVGALLRKGKRYQLEMNSF 334

BLAST of Lag0004853 vs. ExPASy Swiss-Prot
Match: Q9LM18 (F-box protein AUF2 OS=Arabidopsis thaliana OX=3702 GN=AUF2 PE=2 SV=1)

HSP 1 Score: 248.1 bits (632), Expect = 1.5e-64
Identity = 149/330 (45.15%), Postives = 211/330 (63.94%), Query Frame = 0

Query: 15  DAFDCLPDSLILLIFNAVSDVKTLIRCRAVSKRFNSLVPHSDSLSLKVDCVISSDSDENS 74
           D FD LPD +I+ I N V DVKTL+RC ++SKRFNSLVP S+SL+L++D  +S      S
Sbjct: 2   DVFDGLPDPIIVDILNKVGDVKTLLRCSSLSKRFNSLVPQSESLTLRLDHSVSDSPVVTS 61

Query: 75  FLFAFFKSVVKSFFDLISPILVQSESQNSPAQILRQFARIHRLEIEFPTGDPKADRVGGV 134
              + F  +V        PI + + S + P +IL +F RI  L++E P GD K ++   V
Sbjct: 62  IFRSLFNGLVSLLSKPAKPITITTLSPSLPFKILSRFDRIRNLDVELPGGDVKLEKGAAV 121

Query: 135 KWRAEFGITMRSCVILIFRGIRDLDDRAAM-AEDYFDFIGGLKSKVFWTISTLITASARH 194
           KW+AEFG T++SCVI+ FR    +    A+  E   +F+ GLK++V WTIS L+ AS+RH
Sbjct: 122 KWKAEFGKTLKSCVIVAFRSAGTVSSPVAVEGESDAEFVTGLKTRVVWTISALMAASSRH 181

Query: 195 HVLTEVVEEHLEMESLVLRDRGGEGVVVMEKDGLEELRKSTERVGGGLAAAVEGEE--WQ 254
           +++ EVV+EH E+ESLV+RD+  EG VVM ++GL+ELR +  R        VE EE   +
Sbjct: 182 YLMREVVKEHEEIESLVMRDKEREGTVVMNEEGLKELRNTEAR--------VEDEERVVK 241

Query: 255 RTRTRVPSTTVRMRHLPRVELRRGLWMEDATLVVVRPTGDASKDGAADLE-REDADVASR 314
             R+ VPS  + MRH P ++L+ G+ +E ATLV+VRP+G       AD E  +DA++A+ 
Sbjct: 242 NKRSVVPSVRMSMRHAPSLKLKSGICLESATLVIVRPSG-------ADFEVGDDAELATE 301

Query: 315 AFEEGENVYREAVKALLKKGRRYQLEMNSF 341
           AF  G+ +Y EAV ALL K ++  LEMNSF
Sbjct: 302 AF-VGDCMYGEAVVALL-KCKKNALEMNSF 314

BLAST of Lag0004853 vs. ExPASy Swiss-Prot
Match: Q9C9S2 (F-box protein AUF1 OS=Arabidopsis thaliana OX=3702 GN=AUF1 PE=1 SV=1)

HSP 1 Score: 246.9 bits (629), Expect = 3.3e-64
Identity = 156/347 (44.96%), Postives = 215/347 (61.96%), Query Frame = 0

Query: 15  DAFDCLPDSLILLIFNAVSDVKTLIRCRAVSKRFNSLVPHSDSLSLKVDCVI---SSDSD 74
           DAFD +PD +++ I N V DVKTLIRCR+VSKRFNSL   S+SL L++D ++    SDS+
Sbjct: 2   DAFDAIPDPVVIDILNRVGDVKTLIRCRSVSKRFNSLATQSESLLLQLDQILGATESDSE 61

Query: 75  ENSFLFAFFKSVVKSFFDLISPIL--------VQSESQNSPAQILRQFARIHRLEIEFPT 134
            +S + +FF+S+ KS   L+ PI         + + S  +PAQIL  F RI  LE+E   
Sbjct: 62  IDSPIASFFRSLFKSIHGLLPPIFSKPANSDEILTRSPKTPAQILSGFERIRNLEVELYG 121

Query: 135 GDPKADRVGGVKWRAEFGITMRSCVILIFRGIRDLDDRAAMA--------EDYFDFIGGL 194
           GD K ++   VKW+AEFG T++SCVI+ FR        A  A        E   +F+ GL
Sbjct: 122 GDVKLEKGAAVKWKAEFGKTLKSCVIVAFRSATVNTSAATEAAAVVDGVVESDSEFVCGL 181

Query: 195 KSKVFWTISTLITASARHHVLTEVVEEHLEMESLVLRDRGGEGVVVMEKDGLEELRKSTE 254
           K++V WTIS L+ AS RH+++ ++V++H EME L++RD  GEG VVM+  G++E R+ TE
Sbjct: 182 KTRVVWTISALMAASTRHYLMRDLVKDHKEMEKLIVRDSDGEGTVVMDAAGMKEYRE-TE 241

Query: 255 RVGGGLAAAVEGEEWQRTRTRVPSTTVRMRHLPRVELRRGLWMEDATLVVVRPTGDASKD 314
             G    +   GE     RT VPS  + MRH P + L+ G+ +E ATLVVVRPTG AS D
Sbjct: 242 VRGDNKESERVGE-----RTVVPSVRMSMRHAPSLMLKSGICLEAATLVVVRPTGVASDD 301

Query: 315 GAADLEREDADVASRAFE-EGEN-VYREAVKALLKKGRRYQLEMNSF 341
                   D ++ + AF  +G++ +Y EAV ALLK+ RR  LEMNSF
Sbjct: 302 -------NDVELVTEAFAGDGDDCMYGEAVTALLKR-RRNVLEMNSF 334

BLAST of Lag0004853 vs. ExPASy Swiss-Prot
Match: O49508 (F-box protein At4g18380 OS=Arabidopsis thaliana OX=3702 GN=At4g18380 PE=2 SV=1)

HSP 1 Score: 183.3 bits (464), Expect = 4.5e-45
Identity = 140/384 (36.46%), Postives = 201/384 (52.34%), Query Frame = 0

Query: 15  DAFDCLPDSLILLIFNAVSDVKTLIRCRAVSKRFNSLVPHSDSLSLKVDCVISSDSDENS 74
           D FD LPDS++LLIFN + DVK L RC  VSKRF+SL+P  +++ ++VDCVI SD D +S
Sbjct: 23  DHFDNLPDSILLLIFNNIGDVKALGRCSVVSKRFHSLIPQVENVFVRVDCVI-SDDDSSS 82

Query: 75  FL-------------FAFFKSVVKSFFDL-----------------ISPILVQ------- 134
            L              A F+ V K    L                  S +L+        
Sbjct: 83  LLSDKPRSASAASPFSAIFRLVFKPLQALGQFLKRSGSSSLPSGSSPSSLLISGGDDGEI 142

Query: 135 ---SESQNSPAQILRQFARIHRLEIEFPTGDPKADRVGGVKWRAEFGITMRSCVIL---- 194
                + +SP Q+L+ F  I  L+IE P+G+   D    +KWRAEFG T+ +CVIL    
Sbjct: 143 EQGGVTHHSPTQVLKNFDEIKFLKIELPSGELGIDDGVLLKWRAEFGSTLENCVILGASS 202

Query: 195 ------------IFRGIRDLDDRAAMAEDYFDFIGGLKSKVFWTISTLITASARHHVLTE 254
                           +  ++D  ++ E ++   GGLK +V WTIS+LI ASARH++L  
Sbjct: 203 VIPPTNSDKTEASSAPVAAVEDNGSIPESFYT-NGGLKLRVVWTISSLIAASARHYLLQP 262

Query: 255 VVEEHLEMESLVLRDRGGEGVVVMEKDGLEELRKSTERVGGGLAAAVEGEEWQRTRTRVP 314
           ++ EH  ++SLVL D  G+GV+ M +D LEELR         L+A+   +     RT VP
Sbjct: 263 IIAEHKTLDSLVLTDVDGQGVLCMNRDQLEELRVKP------LSASSASK-----RTLVP 322

Query: 315 STTVRMRHLPRVELRRGLWMEDATLVVVRPTGDASKDGAADLEREDADVA--SRAFEEGE 341
           +  +R+ + P +EL  G  ++ ATLV +RP         ++ ++E  DV+  S AF+E  
Sbjct: 323 ALNMRLWYAPSLELPDGTVLKGATLVAIRP---------SESKKEVCDVSWVSSAFDE-- 380

BLAST of Lag0004853 vs. ExPASy Swiss-Prot
Match: Q9FNK5 (F-box protein At5g46170 OS=Arabidopsis thaliana OX=3702 GN=At5g46170 PE=2 SV=1)

HSP 1 Score: 182.2 bits (461), Expect = 9.9e-45
Identity = 140/398 (35.18%), Postives = 194/398 (48.74%), Query Frame = 0

Query: 15  DAFDCLPDSLILLIFNAVSDVKTLIRCRAVSKRFNSLVPHSDSLSLKVDCVISSD----- 74
           D FD LPDS++LL+FN + DVK L RC  VS+RF+SLVP  D++ ++VDCVIS D     
Sbjct: 25  DHFDHLPDSILLLVFNKIGDVKALGRCCVVSRRFHSLVPQVDNVVVRVDCVISDDDSSSL 84

Query: 75  --------SDENSFLFAFFKSVVKSFFDLISPILVQSE---------------------- 134
                   S   SF  A F+ VV     ++ P+    +                      
Sbjct: 85  SSIKSRSGSSAGSF-SAIFRLVVGG---IVKPLQALGQFLGTKRSSSSCGGSGSSSSSLS 144

Query: 135 ---------------SQNSPAQILRQFARIHRLEIEFPTGDPKADRVGGVKWRAEFGITM 194
                          + +SP Q+L+ F  I  L IE P+G+   D    +KWRAEFG T+
Sbjct: 145 ISGDDDGGEIEQGGVTHHSPTQVLKNFDEIRYLRIELPSGELGIDDGVLLKWRAEFGSTL 204

Query: 195 RSCVILIFRGI----------------------RDLDDRAAMAEDYFDFIGGLKSKVFWT 254
            +CVIL    +                         DD  ++ E ++   GGLK +V WT
Sbjct: 205 DNCVILGASSVIPPNPMRVSQACDTTTVVEAPGSGSDDNGSIPESFYT-NGGLKLRVVWT 264

Query: 255 ISTLITASARHHVLTEVVEEHLEMESLVLRDRGGEGVVVMEKDGLEELRKSTERVGGGLA 314
           IS+LI ASARH++L  ++ EH  ++SLVL D  G+GV+ M +D LEELR         LA
Sbjct: 265 ISSLIAASARHYLLQPIIAEHKTLDSLVLTDSDGQGVLCMNRDQLEELRVKP------LA 324

Query: 315 AAVEGEEWQRTRTRVPSTTVRMRHLPRVELRRGLWMEDATLVVVRPTGDASKDGAADLER 341
           A+   +     RT VP+  +R+ + P +EL  G  ++ ATLV +RP+        +  E 
Sbjct: 325 ASSASK-----RTLVPALNMRLWYAPTLELPDGTVLKGATLVAIRPS-------ESKKEV 384

BLAST of Lag0004853 vs. ExPASy Swiss-Prot
Match: Q9C534 (F-box protein At1g30200 OS=Arabidopsis thaliana OX=3702 GN=At1g30200 PE=2 SV=1)

HSP 1 Score: 181.8 bits (460), Expect = 1.3e-44
Identity = 136/378 (35.98%), Postives = 197/378 (52.12%), Query Frame = 0

Query: 15  DAFDCLPDSLILLIFNAVSDVKTLIRCRAVSKRFNSLVPHSDSLSLKVDCVISSD----S 74
           D FD LPDSL+LLIF+ V+DVK L RC  VS+RF+SLVP  +++ ++VDCVIS D    S
Sbjct: 24  DHFDLLPDSLLLLIFDKVADVKDLGRCCIVSRRFHSLVPFVENVLVRVDCVISDDDSSSS 83

Query: 75  DEN--------------------SFLFAFFKSVVKSFFDLISPILVQSES---------- 134
           DEN                    S LF    + +   F ++  IL    S          
Sbjct: 84  DENHRFSLNTASISDAGGAGGSFSALFRLVFAPIFKPFQMLGQILGPKRSSSSFDASFSA 143

Query: 135 --------QNSPAQILRQFARIHRLEIEFPTGDPKADRVGGVKWRAEFGITMRSCVILIF 194
                    +SP Q+L+ F  I  L+IE PTG+   +    +KWRA+FG T+ +C+IL  
Sbjct: 144 INDEIGVTHHSPTQVLKNFGEIRFLKIELPTGELGIEDGILLKWRADFGSTLDNCMILGA 203

Query: 195 RGI----------RDLDDRAAMAEDYFDFIGGLKSKVFWTISTLITASARHHVLTEVVEE 254
             +            +D+      + F   GGLK +V WTIS+LI ASARH++L  ++ E
Sbjct: 204 SSVIQSNSVKNHENSVDEDNGNIPESFYTNGGLKLRVVWTISSLIAASARHYLLQPIINE 263

Query: 255 HLEMESLVLRDRGGEGVVVMEKDGLEELRKSTERVGGGLAAAVEGEEWQRTRTRVPSTTV 314
           H  ++ LVL D  G+GV+ M ++ LEELR +       L+A+   +     RT VP+  +
Sbjct: 264 HKSLDRLVLSDADGQGVLCMNREQLEELRVTP------LSASSASK-----RTLVPALNM 323

Query: 315 RMRHLPRVELRRGLWMEDATLVVVRPTGDASKDGAADLEREDADVASRAFEEGENVYREA 341
           R+ + P ++L  G  ++ ATLV +RP+ ++ K+        DA   S AFEE    +   
Sbjct: 324 RLWYAPELDLPDGTVLKGATLVAIRPS-ESKKEVC------DASWLSDAFEE---PFGTV 379

BLAST of Lag0004853 vs. ExPASy TrEMBL
Match: A0A6J1KRK0 (F-box protein At1g78100-like OS=Cucurbita maxima OX=3661 GN=LOC111496576 PE=4 SV=1)

HSP 1 Score: 501.9 bits (1291), Expect = 2.1e-138
Identity = 276/346 (79.77%), Postives = 302/346 (87.28%), Query Frame = 0

Query: 1   MAERQDSSSIHHLDDAFDCLPDSLILLIFNAVSDVKTLIRCRAVSKRFNSLVPHSDSLSL 60
           MAE QDSS I H+DDAFD LPDS+IL+IFN+VSDVKTLIRCRAVSKRFNSLVPHSDSL L
Sbjct: 1   MAEGQDSSLI-HIDDAFDSLPDSIILMIFNSVSDVKTLIRCRAVSKRFNSLVPHSDSLFL 60

Query: 61  KVDCVIS--SDSDENSFLFAFFKSVVKSFFDLISPILVQSESQNSPAQILRQFARIHRLE 120
           KVDCVIS  SDSDENSFL+ FFKSVVKSFFDLISPILVQSESQNSPAQILRQF RI RLE
Sbjct: 61  KVDCVISSDSDSDENSFLYGFFKSVVKSFFDLISPILVQSESQNSPAQILRQFIRIQRLE 120

Query: 121 IEFPTGDPKADRVGGVKWRAEFGITMRSCVILIFRGIR-DLDDRAAMAEDYFDFIGGLKS 180
           IEFPTGD K +R+GGVKWRAEFGIT+RSCVIL+FRGIR +  D  A+A D FDFI  LKS
Sbjct: 121 IEFPTGDLKVERIGGVKWRAEFGITLRSCVILLFRGIRYESYDETAVA-DGFDFIEELKS 180

Query: 181 KVFWTISTLITASARHHVLTEVVEEHLEMESLVLRDRGGEGVVVMEKDGLEELRKSTERV 240
           KVF TISTLITASARHHVL E++EEHLEMESLVLRDR GEGVV M+KDGL+E RKS ER 
Sbjct: 181 KVFLTISTLITASARHHVLAELIEEHLEMESLVLRDREGEGVVEMDKDGLDEWRKSRERS 240

Query: 241 ---GGGLAAAVEGEEWQRTRTRVPSTTVRMRHLPRVELRRGLWMEDATLVVVRPTGDASK 300
              GGG+AA  EG EW+RTRTRVPSTTVRMRHLPRVELRRG+W+EDATLVVVRPT +ASK
Sbjct: 241 GEDGGGVAA--EGGEWRRTRTRVPSTTVRMRHLPRVELRRGMWIEDATLVVVRPTTNASK 300

Query: 301 DGAADLEREDADVASRAFEEGENVYREAVKALLKKGRRYQLEMNSF 341
           D   D+E+E+ +VA R F EG++VY EAV ALLKKGRRYQLEMNSF
Sbjct: 301 DKTDDMEKENDEVALRTF-EGDDVYGEAVGALLKKGRRYQLEMNSF 341

BLAST of Lag0004853 vs. ExPASy TrEMBL
Match: A0A6J1GI01 (F-box protein At1g22220-like OS=Cucurbita moschata OX=3662 GN=LOC111454360 PE=4 SV=1)

HSP 1 Score: 500.0 bits (1286), Expect = 8.0e-138
Identity = 274/344 (79.65%), Postives = 299/344 (86.92%), Query Frame = 0

Query: 1   MAERQDSSSIHHLDDAFDCLPDSLILLIFNAVSDVKTLIRCRAVSKRFNSLVPHSDSLSL 60
           MAE QDSS I H+DDAFD LPDS+ILLIFN+VSDVKTLIRCRAVSKRFNSLVPHSDSL L
Sbjct: 1   MAEGQDSSLI-HIDDAFDSLPDSIILLIFNSVSDVKTLIRCRAVSKRFNSLVPHSDSLFL 60

Query: 61  KVDCVIS--SDSDENSFLFAFFKSVVKSFFDLISPILVQSESQNSPAQILRQFARIHRLE 120
           KVDCVIS  SDSDENSFL+ FFKSVVKSFFDLISPILVQSESQNSPAQILRQF RI RLE
Sbjct: 61  KVDCVISSDSDSDENSFLYGFFKSVVKSFFDLISPILVQSESQNSPAQILRQFIRIQRLE 120

Query: 121 IEFPTGDPKADRVGGVKWRAEFGITMRSCVILIFRGIR-DLDDRAAMAEDYFDFIGGLKS 180
           IEFPTGD K +R+GGVKWRAEFGIT+RSCVIL+FRGIR +  D  A+A D FDFI  LKS
Sbjct: 121 IEFPTGDLKVERIGGVKWRAEFGITLRSCVILLFRGIRYESYDETAVA-DGFDFIEELKS 180

Query: 181 KVFWTISTLITASARHHVLTEVVEEHLEMESLVLRDRGGEGVVVMEKDGLEELRKSTERV 240
           KVF TISTLITASARHHVL EV+EEHLE++SLVLRDR GEGVV M+KDGL+E RKS +R 
Sbjct: 181 KVFLTISTLITASARHHVLAEVIEEHLEVDSLVLRDREGEGVVEMDKDGLDEWRKSRDRS 240

Query: 241 G-GGLAAAVEGEEWQRTRTRVPSTTVRMRHLPRVELRRGLWMEDATLVVVRPTGDASKDG 300
           G  G   A EG EW+RTRTRVPSTTVRMRHLPRVELRRG+WMEDATLVVVRPT +ASKD 
Sbjct: 241 GEDGGGVAGEGGEWRRTRTRVPSTTVRMRHLPRVELRRGMWMEDATLVVVRPTTNASKDK 300

Query: 301 AADLEREDADVASRAFEEGENVYREAVKALLKKGRRYQLEMNSF 341
             D+E+E+ +VA R F EG++VY EAV ALLKKGRRYQLEMNSF
Sbjct: 301 TDDMEKENDEVALRTF-EGDDVYGEAVGALLKKGRRYQLEMNSF 341

BLAST of Lag0004853 vs. ExPASy TrEMBL
Match: A0A1S3BC00 (F-box protein At1g22220 OS=Cucumis melo OX=3656 GN=LOC103488404 PE=4 SV=1)

HSP 1 Score: 453.4 bits (1165), Expect = 8.5e-124
Identity = 255/345 (73.91%), Postives = 283/345 (82.03%), Query Frame = 0

Query: 7   SSSIHHLD-DAFDCLPDSLILLIFNAVSDVKTLIRCRAVSKRFNSLVPHSDSLSLKVDCV 66
           SS IH  D D FD LPDSLILLIFN+VSDVKTLIRCRAVSKRFNSLVPHSDSLSL VDCV
Sbjct: 17  SSMIHTNDHDGFDSLPDSLILLIFNSVSDVKTLIRCRAVSKRFNSLVPHSDSLSLTVDCV 76

Query: 67  IS------SDSDENSFLFAFFKSVVKSFFDLISPILVQSESQNSPAQILRQFARIHRLEI 126
           IS      SDSD+NSFL +FFKSVVKSF DL+SPILVQSE+QNSPAQILRQF +I  L+I
Sbjct: 77  ISSDSDSDSDSDQNSFLLSFFKSVVKSFLDLLSPILVQSETQNSPAQILRQFLQIQHLQI 136

Query: 127 EFPTGDPKADRVGGVKWRAEFGITMRSCVILIFRGIRDLDDRAAMAED----YFDFIGGL 186
           EFPT D K +RV  VKWRAEFG  ++SCVILIFR IR    + A+ ED      DFIGGL
Sbjct: 137 EFPTTDLKVERV--VKWRAEFGDALKSCVILIFREIR----KGAIEEDDVDYNLDFIGGL 196

Query: 187 KSKVFWTISTLITASARHHVLTEVVEEHLEMESLVLRDRGGEGVVVMEKDGLEELRKSTE 246
           KSKVF TISTLITASARHHVL EVVEEHLEMESLVLRDRGGEGVVVMEK GLEELR+   
Sbjct: 197 KSKVFMTISTLITASARHHVLGEVVEEHLEMESLVLRDRGGEGVVVMEKKGLEELRRWRM 256

Query: 247 RVGGGLAAAVEGEEWQRTRTRVPSTTVRMRHLPRVELRRGLWMEDATLVVVRPTGDASKD 306
             GG +A      EW+RTRTRVPSTTVRMRH  RVE+RRG+WMEDATLVVVRP+G+  K 
Sbjct: 257 GDGGEVA------EWRRTRTRVPSTTVRMRHRGRVEVRRGMWMEDATLVVVRPSGNCRKS 316

Query: 307 GAADLEREDADVASRAFEEGENVYREAVKALLKKGRRYQLEMNSF 341
           G  D+++EDA+VA +AF EG++VYREAV+ALLKKG+RYQLEMNSF
Sbjct: 317 GDGDVDKEDAEVAVKAF-EGDDVYREAVEALLKKGKRYQLEMNSF 348

BLAST of Lag0004853 vs. ExPASy TrEMBL
Match: A0A5A7VGI7 (F-box protein OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold82G001430 PE=4 SV=1)

HSP 1 Score: 450.3 bits (1157), Expect = 7.2e-123
Identity = 253/342 (73.98%), Postives = 281/342 (82.16%), Query Frame = 0

Query: 10  IHHLD-DAFDCLPDSLILLIFNAVSDVKTLIRCRAVSKRFNSLVPHSDSLSLKVDCVIS- 69
           IH  D D FD LPDSLILLIFN+VSDVKTLIRCRAVSKRFNSLVPHSDSLSL VDCVIS 
Sbjct: 2   IHTNDHDGFDSLPDSLILLIFNSVSDVKTLIRCRAVSKRFNSLVPHSDSLSLTVDCVISS 61

Query: 70  -----SDSDENSFLFAFFKSVVKSFFDLISPILVQSESQNSPAQILRQFARIHRLEIEFP 129
                SDSD+NSFL +FFKSVVKSF DL+SPILVQSE+QNSPAQILRQF +I  L+IEFP
Sbjct: 62  DSDSDSDSDQNSFLLSFFKSVVKSFLDLLSPILVQSETQNSPAQILRQFLQIQHLQIEFP 121

Query: 130 TGDPKADRVGGVKWRAEFGITMRSCVILIFRGIRDLDDRAAMAED----YFDFIGGLKSK 189
           T D K +RV  VKWRAEFG  ++SCVILIFR IR    + A+ ED      DFIGGLKSK
Sbjct: 122 TTDLKVERV--VKWRAEFGDALKSCVILIFREIR----KGAIEEDDVDYNLDFIGGLKSK 181

Query: 190 VFWTISTLITASARHHVLTEVVEEHLEMESLVLRDRGGEGVVVMEKDGLEELRKSTERVG 249
           VF TISTLITASARHHVL EVVEEHLEMESLVLRDRGGEGVVVMEK GLEELR+     G
Sbjct: 182 VFMTISTLITASARHHVLGEVVEEHLEMESLVLRDRGGEGVVVMEKKGLEELRRWRMGDG 241

Query: 250 GGLAAAVEGEEWQRTRTRVPSTTVRMRHLPRVELRRGLWMEDATLVVVRPTGDASKDGAA 309
           G +A      EW+RTRTRVPSTTVRMRH  RVE+RRG+WMEDATLVVVRP+G+  K G  
Sbjct: 242 GEVA------EWRRTRTRVPSTTVRMRHRGRVEVRRGMWMEDATLVVVRPSGNCRKSGDG 301

Query: 310 DLEREDADVASRAFEEGENVYREAVKALLKKGRRYQLEMNSF 341
           D+++EDA+VA +AF EG++VYREAV+ALLKKG+RYQLEMNSF
Sbjct: 302 DVDKEDAEVAVKAF-EGDDVYREAVEALLKKGKRYQLEMNSF 330

BLAST of Lag0004853 vs. ExPASy TrEMBL
Match: A0A0A0LN04 (F-box domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_2G307320 PE=4 SV=1)

HSP 1 Score: 448.0 bits (1151), Expect = 3.6e-122
Identity = 247/334 (73.95%), Postives = 279/334 (83.53%), Query Frame = 0

Query: 15  DAFDCLPDSLILLIFNAVSDVKTLIRCRAVSKRFNSLVPHSDSLSLKVDCVISSDSD--- 74
           DAF+ LPDSLILLIFN+VSDVKTLIRCRAVSKRFNSLVPHSDSLSLKVDCVISSDSD   
Sbjct: 30  DAFESLPDSLILLIFNSVSDVKTLIRCRAVSKRFNSLVPHSDSLSLKVDCVISSDSDSDS 89

Query: 75  -ENSFLFAFFKSVVKSFFDLISPILVQSESQNSPAQILRQFARIHRLEIEFPTGDPKADR 134
            +NSFL +FFKSV+KSF DL+SPIL+QSESQNSPAQILRQF RI  L+IEFPT D K +R
Sbjct: 90  HQNSFLLSFFKSVLKSFLDLLSPILLQSESQNSPAQILRQFRRIQHLQIEFPTTDLKVER 149

Query: 135 VGGVKWRAEFGITMRSCVILIFRGIRDLDDRAAMAED----YFDFIGGLKSKVFWTISTL 194
           V  VKWRAEFG  ++SCVILIFR IR    + A+ ED      DFIGGLKSKVF TIST+
Sbjct: 150 V--VKWRAEFGDALKSCVILIFREIR----KGAIEEDDVDSDLDFIGGLKSKVFMTISTV 209

Query: 195 ITASARHHVLTEVVEEHLEMESLVLRDRGGEGVVVMEKDGLEELRKSTERVGGGLAAAVE 254
           ITASARHHVL EVVEEHLEMESL LRDRGGEGVVVMEK GLEELR+     GG +A    
Sbjct: 210 ITASARHHVLGEVVEEHLEMESLALRDRGGEGVVVMEKKGLEELRRWRMGDGGEVA---- 269

Query: 255 GEEWQRTRTRVPSTTVRMRHLPRVELRRGLWMEDATLVVVRPTGDASKDGAADLEREDAD 314
             EW+RTRTRVPSTTVRMRH  RVE+RRG+WMEDATLVVV+P+G+  K G  ++++EDA+
Sbjct: 270 --EWRRTRTRVPSTTVRMRHKGRVEVRRGMWMEDATLVVVKPSGNGRKSGDGEVDKEDAE 329

Query: 315 VASRAFEEGENVYREAVKALLKKGRRYQLEMNSF 341
           VA RAF EG++VYREAV+ALL+KG+RYQLEMNSF
Sbjct: 330 VAVRAF-EGDDVYREAVEALLRKGKRYQLEMNSF 350

BLAST of Lag0004853 vs. TAIR 10
Match: AT1G22220.1 (F-box family protein )

HSP 1 Score: 248.1 bits (632), Expect = 1.0e-65
Identity = 149/330 (45.15%), Postives = 211/330 (63.94%), Query Frame = 0

Query: 15  DAFDCLPDSLILLIFNAVSDVKTLIRCRAVSKRFNSLVPHSDSLSLKVDCVISSDSDENS 74
           D FD LPD +I+ I N V DVKTL+RC ++SKRFNSLVP S+SL+L++D  +S      S
Sbjct: 2   DVFDGLPDPIIVDILNKVGDVKTLLRCSSLSKRFNSLVPQSESLTLRLDHSVSDSPVVTS 61

Query: 75  FLFAFFKSVVKSFFDLISPILVQSESQNSPAQILRQFARIHRLEIEFPTGDPKADRVGGV 134
              + F  +V        PI + + S + P +IL +F RI  L++E P GD K ++   V
Sbjct: 62  IFRSLFNGLVSLLSKPAKPITITTLSPSLPFKILSRFDRIRNLDVELPGGDVKLEKGAAV 121

Query: 135 KWRAEFGITMRSCVILIFRGIRDLDDRAAM-AEDYFDFIGGLKSKVFWTISTLITASARH 194
           KW+AEFG T++SCVI+ FR    +    A+  E   +F+ GLK++V WTIS L+ AS+RH
Sbjct: 122 KWKAEFGKTLKSCVIVAFRSAGTVSSPVAVEGESDAEFVTGLKTRVVWTISALMAASSRH 181

Query: 195 HVLTEVVEEHLEMESLVLRDRGGEGVVVMEKDGLEELRKSTERVGGGLAAAVEGEE--WQ 254
           +++ EVV+EH E+ESLV+RD+  EG VVM ++GL+ELR +  R        VE EE   +
Sbjct: 182 YLMREVVKEHEEIESLVMRDKEREGTVVMNEEGLKELRNTEAR--------VEDEERVVK 241

Query: 255 RTRTRVPSTTVRMRHLPRVELRRGLWMEDATLVVVRPTGDASKDGAADLE-REDADVASR 314
             R+ VPS  + MRH P ++L+ G+ +E ATLV+VRP+G       AD E  +DA++A+ 
Sbjct: 242 NKRSVVPSVRMSMRHAPSLKLKSGICLESATLVIVRPSG-------ADFEVGDDAELATE 301

Query: 315 AFEEGENVYREAVKALLKKGRRYQLEMNSF 341
           AF  G+ +Y EAV ALL K ++  LEMNSF
Sbjct: 302 AF-VGDCMYGEAVVALL-KCKKNALEMNSF 314

BLAST of Lag0004853 vs. TAIR 10
Match: AT1G78100.1 (F-box family protein )

HSP 1 Score: 246.9 bits (629), Expect = 2.3e-65
Identity = 156/347 (44.96%), Postives = 215/347 (61.96%), Query Frame = 0

Query: 15  DAFDCLPDSLILLIFNAVSDVKTLIRCRAVSKRFNSLVPHSDSLSLKVDCVI---SSDSD 74
           DAFD +PD +++ I N V DVKTLIRCR+VSKRFNSL   S+SL L++D ++    SDS+
Sbjct: 2   DAFDAIPDPVVIDILNRVGDVKTLIRCRSVSKRFNSLATQSESLLLQLDQILGATESDSE 61

Query: 75  ENSFLFAFFKSVVKSFFDLISPIL--------VQSESQNSPAQILRQFARIHRLEIEFPT 134
            +S + +FF+S+ KS   L+ PI         + + S  +PAQIL  F RI  LE+E   
Sbjct: 62  IDSPIASFFRSLFKSIHGLLPPIFSKPANSDEILTRSPKTPAQILSGFERIRNLEVELYG 121

Query: 135 GDPKADRVGGVKWRAEFGITMRSCVILIFRGIRDLDDRAAMA--------EDYFDFIGGL 194
           GD K ++   VKW+AEFG T++SCVI+ FR        A  A        E   +F+ GL
Sbjct: 122 GDVKLEKGAAVKWKAEFGKTLKSCVIVAFRSATVNTSAATEAAAVVDGVVESDSEFVCGL 181

Query: 195 KSKVFWTISTLITASARHHVLTEVVEEHLEMESLVLRDRGGEGVVVMEKDGLEELRKSTE 254
           K++V WTIS L+ AS RH+++ ++V++H EME L++RD  GEG VVM+  G++E R+ TE
Sbjct: 182 KTRVVWTISALMAASTRHYLMRDLVKDHKEMEKLIVRDSDGEGTVVMDAAGMKEYRE-TE 241

Query: 255 RVGGGLAAAVEGEEWQRTRTRVPSTTVRMRHLPRVELRRGLWMEDATLVVVRPTGDASKD 314
             G    +   GE     RT VPS  + MRH P + L+ G+ +E ATLVVVRPTG AS D
Sbjct: 242 VRGDNKESERVGE-----RTVVPSVRMSMRHAPSLMLKSGICLEAATLVVVRPTGVASDD 301

Query: 315 GAADLEREDADVASRAFE-EGEN-VYREAVKALLKKGRRYQLEMNSF 341
                   D ++ + AF  +G++ +Y EAV ALLK+ RR  LEMNSF
Sbjct: 302 -------NDVELVTEAFAGDGDDCMYGEAVTALLKR-RRNVLEMNSF 334

BLAST of Lag0004853 vs. TAIR 10
Match: AT4G18380.1 (F-box family protein )

HSP 1 Score: 183.3 bits (464), Expect = 3.2e-46
Identity = 140/384 (36.46%), Postives = 201/384 (52.34%), Query Frame = 0

Query: 15  DAFDCLPDSLILLIFNAVSDVKTLIRCRAVSKRFNSLVPHSDSLSLKVDCVISSDSDENS 74
           D FD LPDS++LLIFN + DVK L RC  VSKRF+SL+P  +++ ++VDCVI SD D +S
Sbjct: 23  DHFDNLPDSILLLIFNNIGDVKALGRCSVVSKRFHSLIPQVENVFVRVDCVI-SDDDSSS 82

Query: 75  FL-------------FAFFKSVVKSFFDL-----------------ISPILVQ------- 134
            L              A F+ V K    L                  S +L+        
Sbjct: 83  LLSDKPRSASAASPFSAIFRLVFKPLQALGQFLKRSGSSSLPSGSSPSSLLISGGDDGEI 142

Query: 135 ---SESQNSPAQILRQFARIHRLEIEFPTGDPKADRVGGVKWRAEFGITMRSCVIL---- 194
                + +SP Q+L+ F  I  L+IE P+G+   D    +KWRAEFG T+ +CVIL    
Sbjct: 143 EQGGVTHHSPTQVLKNFDEIKFLKIELPSGELGIDDGVLLKWRAEFGSTLENCVILGASS 202

Query: 195 ------------IFRGIRDLDDRAAMAEDYFDFIGGLKSKVFWTISTLITASARHHVLTE 254
                           +  ++D  ++ E ++   GGLK +V WTIS+LI ASARH++L  
Sbjct: 203 VIPPTNSDKTEASSAPVAAVEDNGSIPESFYT-NGGLKLRVVWTISSLIAASARHYLLQP 262

Query: 255 VVEEHLEMESLVLRDRGGEGVVVMEKDGLEELRKSTERVGGGLAAAVEGEEWQRTRTRVP 314
           ++ EH  ++SLVL D  G+GV+ M +D LEELR         L+A+   +     RT VP
Sbjct: 263 IIAEHKTLDSLVLTDVDGQGVLCMNRDQLEELRVKP------LSASSASK-----RTLVP 322

Query: 315 STTVRMRHLPRVELRRGLWMEDATLVVVRPTGDASKDGAADLEREDADVA--SRAFEEGE 341
           +  +R+ + P +EL  G  ++ ATLV +RP         ++ ++E  DV+  S AF+E  
Sbjct: 323 ALNMRLWYAPSLELPDGTVLKGATLVAIRP---------SESKKEVCDVSWVSSAFDE-- 380

BLAST of Lag0004853 vs. TAIR 10
Match: AT4G18380.2 (F-box family protein )

HSP 1 Score: 183.3 bits (464), Expect = 3.2e-46
Identity = 140/384 (36.46%), Postives = 201/384 (52.34%), Query Frame = 0

Query: 15  DAFDCLPDSLILLIFNAVSDVKTLIRCRAVSKRFNSLVPHSDSLSLKVDCVISSDSDENS 74
           D FD LPDS++LLIFN + DVK L RC  VSKRF+SL+P  +++ ++VDCVI SD D +S
Sbjct: 23  DHFDNLPDSILLLIFNNIGDVKALGRCSVVSKRFHSLIPQVENVFVRVDCVI-SDDDSSS 82

Query: 75  FL-------------FAFFKSVVKSFFDL-----------------ISPILVQ------- 134
            L              A F+ V K    L                  S +L+        
Sbjct: 83  LLSDKPRSASAASPFSAIFRLVFKPLQALGQFLKRSGSSSLPSGSSPSSLLISGGDDGEI 142

Query: 135 ---SESQNSPAQILRQFARIHRLEIEFPTGDPKADRVGGVKWRAEFGITMRSCVIL---- 194
                + +SP Q+L+ F  I  L+IE P+G+   D    +KWRAEFG T+ +CVIL    
Sbjct: 143 EQGGVTHHSPTQVLKNFDEIKFLKIELPSGELGIDDGVLLKWRAEFGSTLENCVILGASS 202

Query: 195 ------------IFRGIRDLDDRAAMAEDYFDFIGGLKSKVFWTISTLITASARHHVLTE 254
                           +  ++D  ++ E ++   GGLK +V WTIS+LI ASARH++L  
Sbjct: 203 VIPPTNSDKTEASSAPVAAVEDNGSIPESFYT-NGGLKLRVVWTISSLIAASARHYLLQP 262

Query: 255 VVEEHLEMESLVLRDRGGEGVVVMEKDGLEELRKSTERVGGGLAAAVEGEEWQRTRTRVP 314
           ++ EH  ++SLVL D  G+GV+ M +D LEELR         L+A+   +     RT VP
Sbjct: 263 IIAEHKTLDSLVLTDVDGQGVLCMNRDQLEELRVKP------LSASSASK-----RTLVP 322

Query: 315 STTVRMRHLPRVELRRGLWMEDATLVVVRPTGDASKDGAADLEREDADVA--SRAFEEGE 341
           +  +R+ + P +EL  G  ++ ATLV +RP         ++ ++E  DV+  S AF+E  
Sbjct: 323 ALNMRLWYAPSLELPDGTVLKGATLVAIRP---------SESKKEVCDVSWVSSAFDE-- 380

BLAST of Lag0004853 vs. TAIR 10
Match: AT5G46170.1 (F-box family protein )

HSP 1 Score: 182.2 bits (461), Expect = 7.1e-46
Identity = 140/398 (35.18%), Postives = 194/398 (48.74%), Query Frame = 0

Query: 15  DAFDCLPDSLILLIFNAVSDVKTLIRCRAVSKRFNSLVPHSDSLSLKVDCVISSD----- 74
           D FD LPDS++LL+FN + DVK L RC  VS+RF+SLVP  D++ ++VDCVIS D     
Sbjct: 25  DHFDHLPDSILLLVFNKIGDVKALGRCCVVSRRFHSLVPQVDNVVVRVDCVISDDDSSSL 84

Query: 75  --------SDENSFLFAFFKSVVKSFFDLISPILVQSE---------------------- 134
                   S   SF  A F+ VV     ++ P+    +                      
Sbjct: 85  SSIKSRSGSSAGSF-SAIFRLVVGG---IVKPLQALGQFLGTKRSSSSCGGSGSSSSSLS 144

Query: 135 ---------------SQNSPAQILRQFARIHRLEIEFPTGDPKADRVGGVKWRAEFGITM 194
                          + +SP Q+L+ F  I  L IE P+G+   D    +KWRAEFG T+
Sbjct: 145 ISGDDDGGEIEQGGVTHHSPTQVLKNFDEIRYLRIELPSGELGIDDGVLLKWRAEFGSTL 204

Query: 195 RSCVILIFRGI----------------------RDLDDRAAMAEDYFDFIGGLKSKVFWT 254
            +CVIL    +                         DD  ++ E ++   GGLK +V WT
Sbjct: 205 DNCVILGASSVIPPNPMRVSQACDTTTVVEAPGSGSDDNGSIPESFYT-NGGLKLRVVWT 264

Query: 255 ISTLITASARHHVLTEVVEEHLEMESLVLRDRGGEGVVVMEKDGLEELRKSTERVGGGLA 314
           IS+LI ASARH++L  ++ EH  ++SLVL D  G+GV+ M +D LEELR         LA
Sbjct: 265 ISSLIAASARHYLLQPIIAEHKTLDSLVLTDSDGQGVLCMNRDQLEELRVKP------LA 324

Query: 315 AAVEGEEWQRTRTRVPSTTVRMRHLPRVELRRGLWMEDATLVVVRPTGDASKDGAADLER 341
           A+   +     RT VP+  +R+ + P +EL  G  ++ ATLV +RP+        +  E 
Sbjct: 325 ASSASK-----RTLVPALNMRLWYAPTLELPDGTVLKGATLVAIRPS-------ESKKEV 384