Lag0004827 (gene) Sponge gourd (AG‐4) v1

Overview
NameLag0004827
Typegene
OrganismLuffa acutangula (Sponge gourd (AG‐4) v1)
Descriptionphosphopantothenate--cysteine ligase 2-like
Locationchr6: 7526315 .. 7530292 (-)
RNA-Seq ExpressionLag0004827
SyntenyLag0004827
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: polypeptideexonCDS
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGGATGTAAATGGACGGCAAAAAGCGCAAGATGCCCTAGATGCTGAAATCAAATCATTCTTTGATTCAGCTCCCCCTTTGAAGAATATTGAGAGTATCATTGAAAAACTGAAAAAATTTATTGAAGTGAATTCATCGCAAGCTGGCAAGTTCTCTCTGAGTTAATCCCTTAAAGTAGTCTTTTTGCTTTGATTTAGTTGCTATTTCCTAATAATCTACTTAGGGATTACTTTGCTAGTACCTGTATTACTGAACACTTAACAACACTCATGTGAAATGCAAAGTACGAATAGTAAAGTTTAGGAATTTAGATTGTATGTTTATCTAATTAGTTTTATTGGATCAATTTGCTTGTAAATATAGGAACTGAAAGTGCCAGGAAAGTTGTTTGTGTGACATCTGGGGGCACCACGGTTCCATTAGAGCAAAGATGTGTTCGCTATATAGACAACTTCAGCTCAGGTCACAGGGGTGCAGCTTCCACAGAGTAAATGACATTACACTTCAACATGTGTTATACTTGAGATCATACATTGAGTTTGTTTCTTAAGTGATCTTGCTAATTTCTATCAGGTACTTTCTGAAGGCAGGATACTCGGTTATCTTCTTGTATCGAACGTACATACTCATCTTTCCTGGAACTGCATGGTGTAAGAATTATGCTAGGTTAAGGCTTGAAACCCTCCTGGGCTAATTTATGGTTCTGTGATCAGGGGAACCTGTCAACCATATTGCAGTTTGCTTCCTGATGATCCATTTCTGGAGTGTTTTGAGTTCACCCATGAGTCAGGCATACAAGGTTTGTGATTTTCTTTATCTCCAACTTAATCGATGCTCTGCTGGTTACAGCGTTAATGGTGTCATATGTTCCTGACGAGGAATCAATTGCCTGTGTATTCTGTTGGATACATAATATTACCGGAAAACAGCCACTTGTTCATAGGCATACAAGGTTCCTTTCCTTTGAGGGTGACTTTGGGATGACTTCTGAATTTGTGAAGTGTTTTCTTTTCAAAGTTACATAGTTAAAAGTGATTTTTAAAATTTCTAAAAATACTTGGTGCTTTTAAATACTTCTTCCAAAAGTGCTGGTAGAAAAAGTGTTGTACTTAAAAAGCATTACTCTCAAAAGTCATCTCAACCTCATCCTTAGACCCCTTGATACATAATGTTGGAATTTTGTTGTAACGTCAATGTTTGTTATCAAACTCTTTGAATTAGAAACTGATTTTAATTTATCATTTGGTGTACTTTATAACAAAGTTTCTTTTTTTTGTTATCTGGGCCTCAGGGTTAAGTTGCCTCTCTTTTAGCACTTCGACTTTTATTTTATTGATCAATTCTTATAATTCATGGTGCTCATGACTTAAATTCTTTTCAAAATTCAGTTCGCCAGCCATATTCAGAAGCAGTGAAGAAGGCTATCAGTGAGCATCATGCTGTATGGACTACTCTATGTAGTCTCATCACTCAATTTAGATTCTGACTGTTCATTATCTTTTCCATGTATGCCCATAGCTTACTTGGTTGATTAGCAAAATTTGGGATTTGACTATTGTAGGCAGTGGCAGATGGCAAACTTCTGAAACTTCCTTTTACAACCATTTTTGAGTATCTTCAGGTAATTTTCCCTTTTTTGGGTAAAAACACAGGCATTAATTAGGAAGTTTTATGGCTATATTTCTGTGCTAGCTAGCAGAACGATCTATTATTAGAATATATGTCTTGTATTGGTCAAAACTCGTGTCTGTTCAAAATCAGCATACTAAATTATTCAAGTTTTAATGAAAGCATTGATGGGGTTTGCAAGTAAAAATAGGGAAGAAAATGGTTCTTTTGAATTCAAAAAGACACAAATGTTTATCAATATTCATATACCCAAAAGCACGGTCCAATGGATCAAGTAATCATTCCAATTTTTCTCTAGCTTATATTTTTATCAAGTTTATGTAGAAAACATATTAACAGGGTATAAACTTCTTTTAATCTTATTATTTATTTTGCAGATGTTGCACATGATTGCGACATCATTGAGGAGTCTTGGACCCCATGCACTCTTTTATCTTGCTGCGGCTGTCTCTGACTTTTATGTTCCATGGGAGGGCATGGTAATGGTTTAGCTTATAAGTAATTGTTTCCTTTCTCCTCTTAAAGAGAACTCCCGCTCCCCCCTTGACTAAATGCAGGTTGTCATTTTAACACTTCTATATGTCGTCTCTGAACACAGTTCGCATTTTCTTTAATGTGTTTCTAGCTATTTAAAGAATTTCTCCTTAGGAAAGATATCTTAATCGATTTACCATCGGTTCAAATAAAGCGGGATTTTAATGTGTTATTCATTGTGCTTAAATAATTCTTCTCATCTCTAAATTTTCCAACTGTTTTCACAAATTAGGCTTAGCATTTATGTAGCATACATGTGGTATACATCTGATGTGTGTGTGCTCGCATGAGATCTGTAGTCATATAGTAGCCTGTATCTGTCTAAAAGATCAATAGACTTGGACTGTTGTAGATTCATGGATGGCAACATTCATACTTGCATCTCAAATCTTGTTTTAATCAAGAAAATAGAATGTCAAGGAATCGAGGATAATCATGAATTCAAAGTTTTCCTCCTGTTTAAGCAAACGGGGAGTTTGATATTGTAATTGTTGGACAGTGGTTCTCAATCGTCAAATGTTAATTCATTCATTTAGTCATTCATGGAACTACTTTCCGATTTCTGCCTTCATTCATTTTTCTAGTCTTTTCATTTCAGGCAGAGCACAAGATCCAGTCAGGATCAGGACCTTTAGACATGCGACTTGTTCAGGTGCCAAAGATGCTATCAGTGCTGAGGAGTGAATGGGCTCCAACAGCGTACTGTATATCCTTCAAGGTATGTTCTCCAATTGAAACTATGTTAAGATATGCTCAATGTGGGGATACAGACAAAAGCCCCATCTGAAGTTAAAAAATTATGTCCATTTAGCTAAAAAACGAAATGGAGAAATCAACGTACCTCCCAAATTTCCTCCTTATACCTTAGTTCTTTCAGACCACAGTCCATAGGTCCATAGGAGAACTCGAATGGAGTTTTTAGAGAACCATTCTTGTTTTAGCAAATGGCTGCTGCACACAAATTGCTCGAAAGCCAATTGTTAGGGAGATCTTGGCTGAAGTTAAAACAATCAAGAAGCTTCCGTTGGGTAAAAGTCGAGAAAGGGCAATGGAGAACCTGCCCAACATCTAACTAATTAGGGGGAGAGGGTTTTGCAAGTGTTTATTTGCTGAATTTTGTATTTTATATTAGGTCTCATAGAACCCCATAAAAAGACCTTTTTCTTCTTAGGTTGCTTGGCTGTCCAACAACCTTCGAAAATTGGAGAATCCAAAAATCAAATCGCTGATCTTAGTGATGGAATGCCTCAAGCCACACGAGCACTGATCCAACAAGAGCCTGTGAACTAATTTCATTTCTTCCTTTTTTTTCTTATTTGTTAGTGACTGAACTTTGATGACTCTAGGATCAGTTCAGGAGTAACACATTATTTTCACTGCACTCTAGATGTAAAATATAGTTGGCTATGACCCCATAAAATAACAATGTAATACCAGCCATATAAACATTGGAAACAAGGATGCTTCTGCAATGATTATGCCATTATAGCCATTAAGTGTCGTTATGGGTTCTATTTATTTTTCACTACAATTTCTCCTTTGCTCAAACAGCTAGAGACTGATGTAAAAATCCTCCTGGAGAAGGCGAATGATGCTCTGAGAAAGTACAAGATGAATATGGTCATTGCAAATGAACTTTTGACCCGCAAGGAGGAGGTTACTCTTGTAACTGGAAATGAAAATATTCATGTTCGGCGTGATCCGATACGGGTTGGTGACGAAGTGGAAAATCATATTGTCGAACATATTGTTGAGAAGCATTCTGCTTATGTGGACAATTTCGGCCACAAAGCTTCAACAGCCACTGGATCATAG

mRNA sequence

ATGGATGTAAATGGACGGCAAAAAGCGCAAGATGCCCTAGATGCTGAAATCAAATCATTCTTTGATTCAGCTCCCCCTTTGAAGAATATTGAGAGAACTGAAAGTGCCAGGAAAGTTGTTTGTGTGACATCTGGGGGCACCACGGTTCCATTAGAGCAAAGATGTGTTCGCTATATAGACAACTTCAGCTCAGGTCACAGGGGTGCAGCTTCCACAGAGTACTTTCTGAAGGCAGGATACTCGGTTATCTTCTTGTATCGAACGGGAACCTGTCAACCATATTGCAGTTTGCTTCCTGATGATCCATTTCTGGAGTGTTTTGAGTTCACCCATGAGTCAGGCATACAAGTTCGCCAGCCATATTCAGAAGCAGTGAAGAAGGCTATCAGTGAGCATCATGCTGCAGTGGCAGATGGCAAACTTCTGAAACTTCCTTTTACAACCATTTTTGAGTATCTTCAGATGTTGCACATGATTGCGACATCATTGAGGAGTCTTGGACCCCATGCACTCTTTTATCTTGCTGCGGCTGTCTCTGACTTTTATGTTCCATGGGAGGGCATGGCAGAGCACAAGATCCAGTCAGGATCAGGACCTTTAGACATGCGACTTGTTCAGGTGCCAAAGATGCTATCAGTGCTGAGGAGTGAATGGGCTCCAACAGCGTACTGTATATCCTTCAAGCTAGAGACTGATGTAAAAATCCTCCTGGAGAAGGCGAATGATGCTCTGAGAAAGTACAAGATGAATATGGTCATTGCAAATGAACTTTTGACCCGCAAGGAGGAGGTTACTCTTGTAACTGGAAATGAAAATATTCATGTTCGGCGTGATCCGATACGGGTTGGTGACGAAGTGGAAAATCATATTGTCGAACATATTGTTGAGAAGCATTCTGCTTATGTGGACAATTTCGGCCACAAAGCTTCAACAGCCACTGGATCATAG

Coding sequence (CDS)

ATGGATGTAAATGGACGGCAAAAAGCGCAAGATGCCCTAGATGCTGAAATCAAATCATTCTTTGATTCAGCTCCCCCTTTGAAGAATATTGAGAGAACTGAAAGTGCCAGGAAAGTTGTTTGTGTGACATCTGGGGGCACCACGGTTCCATTAGAGCAAAGATGTGTTCGCTATATAGACAACTTCAGCTCAGGTCACAGGGGTGCAGCTTCCACAGAGTACTTTCTGAAGGCAGGATACTCGGTTATCTTCTTGTATCGAACGGGAACCTGTCAACCATATTGCAGTTTGCTTCCTGATGATCCATTTCTGGAGTGTTTTGAGTTCACCCATGAGTCAGGCATACAAGTTCGCCAGCCATATTCAGAAGCAGTGAAGAAGGCTATCAGTGAGCATCATGCTGCAGTGGCAGATGGCAAACTTCTGAAACTTCCTTTTACAACCATTTTTGAGTATCTTCAGATGTTGCACATGATTGCGACATCATTGAGGAGTCTTGGACCCCATGCACTCTTTTATCTTGCTGCGGCTGTCTCTGACTTTTATGTTCCATGGGAGGGCATGGCAGAGCACAAGATCCAGTCAGGATCAGGACCTTTAGACATGCGACTTGTTCAGGTGCCAAAGATGCTATCAGTGCTGAGGAGTGAATGGGCTCCAACAGCGTACTGTATATCCTTCAAGCTAGAGACTGATGTAAAAATCCTCCTGGAGAAGGCGAATGATGCTCTGAGAAAGTACAAGATGAATATGGTCATTGCAAATGAACTTTTGACCCGCAAGGAGGAGGTTACTCTTGTAACTGGAAATGAAAATATTCATGTTCGGCGTGATCCGATACGGGTTGGTGACGAAGTGGAAAATCATATTGTCGAACATATTGTTGAGAAGCATTCTGCTTATGTGGACAATTTCGGCCACAAAGCTTCAACAGCCACTGGATCATAG

Protein sequence

MDVNGRQKAQDALDAEIKSFFDSAPPLKNIERTESARKVVCVTSGGTTVPLEQRCVRYIDNFSSGHRGAASTEYFLKAGYSVIFLYRTGTCQPYCSLLPDDPFLECFEFTHESGIQVRQPYSEAVKKAISEHHAAVADGKLLKLPFTTIFEYLQMLHMIATSLRSLGPHALFYLAAAVSDFYVPWEGMAEHKIQSGSGPLDMRLVQVPKMLSVLRSEWAPTAYCISFKLETDVKILLEKANDALRKYKMNMVIANELLTRKEEVTLVTGNENIHVRRDPIRVGDEVENHIVEHIVEKHSAYVDNFGHKASTATGS
Homology
BLAST of Lag0004827 vs. NCBI nr
Match: XP_023536743.1 (phosphopantothenate--cysteine ligase 2-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 563.1 bits (1450), Expect = 1.5e-156
Identity = 285/330 (86.36%), Postives = 296/330 (89.70%), Query Frame = 0

Query: 1   MDVNGRQKAQDALDAEIKSFFDSAPPLKNIER---------------TESARKVVCVTSG 60
           MDV+G+QKAQ+ALD EIKSFFDSAPPL+NIE                T SARKVVCVTSG
Sbjct: 1   MDVHGQQKAQEALDVEIKSFFDSAPPLRNIENVSEDLKKFIEMNSSGTRSARKVVCVTSG 60

Query: 61  GTTVPLEQRCVRYIDNFSSGHRGAASTEYFLKAGYSVIFLYRTGTCQPYCSLLPDDPFLE 120
           GTTVPLEQRCVRYIDNFSSGHRGAASTEYFLKAGYSVIFLYR GTCQPYCSLLPD PFLE
Sbjct: 61  GTTVPLEQRCVRYIDNFSSGHRGAASTEYFLKAGYSVIFLYRKGTCQPYCSLLPDYPFLE 120

Query: 121 CFEFTHESGIQVRQPYSEAVKKAISEHHAAVADGKLLKLPFTTIFEYLQMLHMIATSLRS 180
           CFEFTHESGIQVRQPYSEAVK AISEHHAAVADG LLKLPFTTIFEYLQ+LHM+ATSLRS
Sbjct: 121 CFEFTHESGIQVRQPYSEAVKWAISEHHAAVADGTLLKLPFTTIFEYLQILHMVATSLRS 180

Query: 181 LGPHALFYLAAAVSDFYVPWEGMAEHKIQSGSGPLDMRLVQVPKMLSVLRSEWAPTAYCI 240
           LGP+ALFYLAAAVSDFYVPWE MAEHKIQSGSGPLDMRLVQVPKMLSVLRSEWAPTAYCI
Sbjct: 181 LGPYALFYLAAAVSDFYVPWESMAEHKIQSGSGPLDMRLVQVPKMLSVLRSEWAPTAYCI 240

Query: 241 SFKLETDVKILLEKANDALRKYKMNMVIANELLTRKEEVTLVTGNENIHVRRDPIRVGDE 300
           SFKLETDVKILLEKAN AL+KYKM+MVIANELLTRKEEVTLVT NENIHVRRDP +VGDE
Sbjct: 241 SFKLETDVKILLEKANAALKKYKMHMVIANELLTRKEEVTLVTDNENIHVRRDPKQVGDE 300

Query: 301 VENHIVEHIVEKHSAYVDNFGHKASTATGS 316
           VE HI+EHIVEKHS YVDN   K S  TGS
Sbjct: 301 VEKHIIEHIVEKHSTYVDNLDQKVSRDTGS 330

BLAST of Lag0004827 vs. NCBI nr
Match: XP_023002782.1 (phosphopantothenate--cysteine ligase 2-like [Cucurbita maxima])

HSP 1 Score: 560.5 bits (1443), Expect = 9.5e-156
Identity = 284/330 (86.06%), Postives = 295/330 (89.39%), Query Frame = 0

Query: 1   MDVNGRQKAQDALDAEIKSFFDSAPPLKNIER---------------TESARKVVCVTSG 60
           MDV+G+QKAQ+ALD EIKSFFDSAPPL+NIE                T SARKVVCVTSG
Sbjct: 1   MDVHGQQKAQEALDVEIKSFFDSAPPLRNIENVSEDLKKFIEMNSSGTRSARKVVCVTSG 60

Query: 61  GTTVPLEQRCVRYIDNFSSGHRGAASTEYFLKAGYSVIFLYRTGTCQPYCSLLPDDPFLE 120
           GTTVPLEQRCVRYIDNFSSGHRGAASTEYFLKAGY VIFLYR GTCQPYCSLLPD PFLE
Sbjct: 61  GTTVPLEQRCVRYIDNFSSGHRGAASTEYFLKAGYLVIFLYRKGTCQPYCSLLPDYPFLE 120

Query: 121 CFEFTHESGIQVRQPYSEAVKKAISEHHAAVADGKLLKLPFTTIFEYLQMLHMIATSLRS 180
           CFEFTHESGIQVRQPYSEAVK AISEHHAAVADG LLKLPFTTIFEYLQ+LHM+ATSLRS
Sbjct: 121 CFEFTHESGIQVRQPYSEAVKWAISEHHAAVADGTLLKLPFTTIFEYLQILHMVATSLRS 180

Query: 181 LGPHALFYLAAAVSDFYVPWEGMAEHKIQSGSGPLDMRLVQVPKMLSVLRSEWAPTAYCI 240
           LGP+ALFYLAAAVSDFYVPWE MAEHKIQSGSGPLDMRLVQVPKMLSVLRSEWAPTAYCI
Sbjct: 181 LGPNALFYLAAAVSDFYVPWESMAEHKIQSGSGPLDMRLVQVPKMLSVLRSEWAPTAYCI 240

Query: 241 SFKLETDVKILLEKANDALRKYKMNMVIANELLTRKEEVTLVTGNENIHVRRDPIRVGDE 300
           SFKLETDVKILLEKAN AL+KYKM+MVIANELLTRKEEVTLVT NENIHVRRDP +VGDE
Sbjct: 241 SFKLETDVKILLEKANAALKKYKMHMVIANELLTRKEEVTLVTDNENIHVRRDPKQVGDE 300

Query: 301 VENHIVEHIVEKHSAYVDNFGHKASTATGS 316
           VE HI+EHIVEKHS YVDN   K S  TGS
Sbjct: 301 VEKHIIEHIVEKHSTYVDNLDQKVSRDTGS 330

BLAST of Lag0004827 vs. NCBI nr
Match: XP_022952050.1 (phosphopantothenate--cysteine ligase 2-like [Cucurbita moschata])

HSP 1 Score: 555.4 bits (1430), Expect = 3.1e-154
Identity = 281/330 (85.15%), Postives = 295/330 (89.39%), Query Frame = 0

Query: 1   MDVNGRQKAQDALDAEIKSFFDSAPPLKNIER---------------TESARKVVCVTSG 60
           MDV+G+QKAQ+ALD EIKSFFDSAPPL+NIE                T SARKVVCVTSG
Sbjct: 1   MDVHGQQKAQEALDVEIKSFFDSAPPLRNIENVSEDLKKFIEMNSSGTRSARKVVCVTSG 60

Query: 61  GTTVPLEQRCVRYIDNFSSGHRGAASTEYFLKAGYSVIFLYRTGTCQPYCSLLPDDPFLE 120
           GTTVPLEQ+CVRYIDNFSSGHRGAASTEYFLKAGYSVIFLYR GTCQPYCSLLPD PFLE
Sbjct: 61  GTTVPLEQKCVRYIDNFSSGHRGAASTEYFLKAGYSVIFLYRKGTCQPYCSLLPDYPFLE 120

Query: 121 CFEFTHESGIQVRQPYSEAVKKAISEHHAAVADGKLLKLPFTTIFEYLQMLHMIATSLRS 180
           CFEFTH SGIQVRQPYSEAVK AISEHHAAVADG LLKLPFTTIFEYLQ+LHM+ATSLRS
Sbjct: 121 CFEFTHGSGIQVRQPYSEAVKWAISEHHAAVADGTLLKLPFTTIFEYLQILHMVATSLRS 180

Query: 181 LGPHALFYLAAAVSDFYVPWEGMAEHKIQSGSGPLDMRLVQVPKMLSVLRSEWAPTAYCI 240
           LGP+ALFYLAAAVSDFYVPWE MAEHKIQSGSGPLDMRLVQVPKMLSVLRSEWAPTAYCI
Sbjct: 181 LGPYALFYLAAAVSDFYVPWESMAEHKIQSGSGPLDMRLVQVPKMLSVLRSEWAPTAYCI 240

Query: 241 SFKLETDVKILLEKANDALRKYKMNMVIANELLTRKEEVTLVTGNENIHVRRDPIRVGDE 300
           SFKLETDVKILLEKAN AL+KYKM+MVIANELLTRKEEVTLVT NENIHVRRDP +VG+E
Sbjct: 241 SFKLETDVKILLEKANAALKKYKMHMVIANELLTRKEEVTLVTDNENIHVRRDPKQVGNE 300

Query: 301 VENHIVEHIVEKHSAYVDNFGHKASTATGS 316
           VE HI+EHIVEKHS YVD+   K S  TGS
Sbjct: 301 VEKHIIEHIVEKHSTYVDDLDQKVSRDTGS 330

BLAST of Lag0004827 vs. NCBI nr
Match: KAG6585277.1 (Phosphopantothenate--cysteine ligase 1, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 552.0 bits (1421), Expect = 3.4e-153
Identity = 280/324 (86.42%), Postives = 290/324 (89.51%), Query Frame = 0

Query: 7   QKAQDALDAEIKSFFDSAPPLKNIER---------------TESARKVVCVTSGGTTVPL 66
           QKAQ+ALD EIKSFFDSAPPL+NIE                T SARKVVCVTSGGTTVPL
Sbjct: 136 QKAQEALDVEIKSFFDSAPPLRNIENVSEDLKKFIEMNSSGTRSARKVVCVTSGGTTVPL 195

Query: 67  EQRCVRYIDNFSSGHRGAASTEYFLKAGYSVIFLYRTGTCQPYCSLLPDDPFLECFEFTH 126
           EQRCVRYIDNFSSGHRGAASTEYFLKAGYSVIFLYR GTCQPYCSLLPD PFLECFEFTH
Sbjct: 196 EQRCVRYIDNFSSGHRGAASTEYFLKAGYSVIFLYRKGTCQPYCSLLPDYPFLECFEFTH 255

Query: 127 ESGIQVRQPYSEAVKKAISEHHAAVADGKLLKLPFTTIFEYLQMLHMIATSLRSLGPHAL 186
           ESGIQVRQPYSEAVK AISEHHAAVADG LLKLPFTTIFEYLQ+LHM+ATSLRSLGP+AL
Sbjct: 256 ESGIQVRQPYSEAVKWAISEHHAAVADGTLLKLPFTTIFEYLQILHMVATSLRSLGPYAL 315

Query: 187 FYLAAAVSDFYVPWEGMAEHKIQSGSGPLDMRLVQVPKMLSVLRSEWAPTAYCISFKLET 246
           FYLAAAVSDFYVPWE MAEHKIQSGSGPLDMRLVQVPKMLSVLRSEWAPTAYCISFKLET
Sbjct: 316 FYLAAAVSDFYVPWESMAEHKIQSGSGPLDMRLVQVPKMLSVLRSEWAPTAYCISFKLET 375

Query: 247 DVKILLEKANDALRKYKMNMVIANELLTRKEEVTLVTGNENIHVRRDPIRVGDEVENHIV 306
           DVKILLEKAN AL+KYKM+MVIANELLTRKEEVTLVT NENIHVRRDP +VGDEVE HI+
Sbjct: 376 DVKILLEKANAALKKYKMHMVIANELLTRKEEVTLVTDNENIHVRRDPKQVGDEVEKHII 435

Query: 307 EHIVEKHSAYVDNFGHKASTATGS 316
           EHIVEKHS YVD+   K S  TGS
Sbjct: 436 EHIVEKHSTYVDDLDQKVSRDTGS 459

BLAST of Lag0004827 vs. NCBI nr
Match: XP_008445369.1 (PREDICTED: phosphopantothenate--cysteine ligase 2-like [Cucumis melo] >XP_008445370.1 PREDICTED: phosphopantothenate--cysteine ligase 2-like [Cucumis melo] >XP_016900047.1 PREDICTED: phosphopantothenate--cysteine ligase 2-like [Cucumis melo] >KAA0064808.1 phosphopantothenate--cysteine ligase 2-like [Cucumis melo var. makuwa])

HSP 1 Score: 543.5 bits (1399), Expect = 1.2e-150
Identity = 276/332 (83.13%), Postives = 291/332 (87.65%), Query Frame = 0

Query: 1   MDVNGRQKAQDALDAEIKSFFDSAPPLKNIE-----------------RTESARKVVCVT 60
           MDVNG QKAQ+ALDAEIKSFF+SAPPL+NIE                   +SARKVVCVT
Sbjct: 1   MDVNGLQKAQEALDAEIKSFFNSAPPLRNIEDIGKDLRKFVEFNSPQAGNQSARKVVCVT 60

Query: 61  SGGTTVPLEQRCVRYIDNFSSGHRGAASTEYFLKAGYSVIFLYRTGTCQPYCSLLPDDPF 120
           SGGTTVPLEQRCVRYIDNFSSGHRGAASTEYFLKAGYSVIFLYR GT QPYCSLLPDDPF
Sbjct: 61  SGGTTVPLEQRCVRYIDNFSSGHRGAASTEYFLKAGYSVIFLYRKGTRQPYCSLLPDDPF 120

Query: 121 LECFEFTHESGIQVRQPYSEAVKKAISEHHAAVADGKLLKLPFTTIFEYLQMLHMIATSL 180
           LEC EFT ESGIQ RQPYSEAVK AISEHHAA+A+G LLKLPFTTIFEYLQMLH++A S+
Sbjct: 121 LECLEFTQESGIQARQPYSEAVKNAISEHHAAIANGTLLKLPFTTIFEYLQMLHLVAISM 180

Query: 181 RSLGPHALFYLAAAVSDFYVPWEGMAEHKIQSGSGPLDMRLVQVPKMLSVLRSEWAPTAY 240
           RS+GPHALFYLAAAVSDFYVPWE MAEHKIQSGSGPLDMRLVQVPKMLS LRSEWAP AY
Sbjct: 181 RSVGPHALFYLAAAVSDFYVPWESMAEHKIQSGSGPLDMRLVQVPKMLSGLRSEWAPMAY 240

Query: 241 CISFKLETDVKILLEKANDALRKYKMNMVIANELLTRKEEVTLVTGNENIHVRRDPIRVG 300
           CISFKLETDVKILLEKA+ ALRKYKM+MVIANELLTRKEEVTLVT NE IHVRRDP  VG
Sbjct: 241 CISFKLETDVKILLEKADAALRKYKMHMVIANELLTRKEEVTLVTTNEKIHVRRDPKLVG 300

Query: 301 DEVENHIVEHIVEKHSAYVDNFGHKASTATGS 316
           D VE HIV+HIV+KHSAYVDNF HK S A+GS
Sbjct: 301 DNVEEHIVKHIVKKHSAYVDNFDHKVSRASGS 332

BLAST of Lag0004827 vs. ExPASy Swiss-Prot
Match: Q8GXR5 (Phosphopantothenate--cysteine ligase 1 OS=Arabidopsis thaliana OX=3702 GN=PPCS1 PE=2 SV=1)

HSP 1 Score: 395.2 bits (1014), Expect = 6.9e-109
Identity = 198/305 (64.92%), Postives = 240/305 (78.69%), Query Frame = 0

Query: 13  LDAEIKSFFDSAPPLKN-----------IERTESA----RKVVCVTSGGTTVPLEQRCVR 72
           ++ EI SFF+S+PPLKN           IE   S     R++VCVTSGGTTVPLEQRCVR
Sbjct: 8   VEDEISSFFESSPPLKNKEEIVANLNQFIELNSSCQGGMRRIVCVTSGGTTVPLEQRCVR 67

Query: 73  YIDNFSSGHRGAASTEYFLKAGYSVIFLYRTGTCQPYCSLLPDDPFLECFEFTHESGIQV 132
           YIDNFSSG+RGAASTE F+KAGY+VIFLYR GTCQPYC  LPDDPFLECFEF     IQV
Sbjct: 68  YIDNFSSGNRGAASTENFVKAGYAVIFLYRRGTCQPYCRYLPDDPFLECFEFPDAKNIQV 127

Query: 133 RQPYSEAVKKAISEHHAAVADGKLLKLPFTTIFEYLQMLHMIATSLRSLGPHALFYLAAA 192
              +S AVK A+ +  AAVA+G+LLKLPF+TI+EYLQML +IAT L+ +GP ++FYLAAA
Sbjct: 128 HGSHSGAVKMAVMDQQAAVAEGRLLKLPFSTIYEYLQMLRLIATILKDVGPCSMFYLAAA 187

Query: 193 VSDFYVPWEGMAEHKIQSGSGPLDMRLVQVPKMLSVLRSEWAPTAYCISFKLETDVKILL 252
           VSDFYVPW  M EHKI+SGSGPLD+RL QVPKMLS+LRS WAP A+CISFKLETD KILL
Sbjct: 188 VSDFYVPWLSMTEHKIESGSGPLDIRLAQVPKMLSILRSNWAPKAFCISFKLETDSKILL 247

Query: 253 EKANDALRKYKMNMVIANELLTRKEEVTLVTGNENIHVRRDPIRVGDEVENHIVEHIVEK 303
           EKA  AL+KYK++ V+ANELLTRKEEV +V+ + N+ VRRD  +    VE++++  +V++
Sbjct: 248 EKATKALQKYKVHAVVANELLTRKEEVVVVSSSGNVVVRRDSNKPESIVEDNLIRLLVDR 307

BLAST of Lag0004827 vs. ExPASy Swiss-Prot
Match: Q9LZM3 (Phosphopantothenate--cysteine ligase 2 OS=Arabidopsis thaliana OX=3702 GN=PPCS2 PE=2 SV=2)

HSP 1 Score: 394.8 bits (1013), Expect = 9.0e-109
Identity = 193/304 (63.49%), Postives = 241/304 (79.28%), Query Frame = 0

Query: 13  LDAEIKSFFDSAPPLKNIER--------------TESARKVVCVTSGGTTVPLEQRCVRY 72
           ++ EI SFF+S+PP KN+E               ++  R++VCVTSGGTTVPLEQRCVRY
Sbjct: 1   MEDEISSFFESSPPQKNMEEILENLNEFIKLNSSSQGGRRIVCVTSGGTTVPLEQRCVRY 60

Query: 73  IDNFSSGHRGAASTEYFLKAGYSVIFLYRTGTCQPYCSLLPDDPFLECFEFTHESGIQVR 132
           IDNFSSG+RGAASTE F+KAGY+VIFLYR GTCQPYC  LPDDPFLECFEF+ ++ IQV 
Sbjct: 61  IDNFSSGNRGAASTENFVKAGYAVIFLYRRGTCQPYCRSLPDDPFLECFEFSDKTNIQVH 120

Query: 133 QPYSEAVKKAISEHHAAVADGKLLKLPFTTIFEYLQMLHMIATSLRSLGPHALFYLAAAV 192
             + EAVK A+ +   AVA+G LLKLPF+TI+EYLQML +IA +L+ +GP ++FYLAAAV
Sbjct: 121 TSHLEAVKMAVMDQQTAVAEGSLLKLPFSTIYEYLQMLRLIAEALKDVGPCSMFYLAAAV 180

Query: 193 SDFYVPWEGMAEHKIQSGSGPLDMRLVQVPKMLSVLRSEWAPTAYCISFKLETDVKILLE 252
           SDFYVPW+ M EHKI+SGSGPLD+RL QVPKMLSVLRS WAP A+CISFKLETD KIL+E
Sbjct: 181 SDFYVPWKSMTEHKIESGSGPLDIRLAQVPKMLSVLRSNWAPKAFCISFKLETDSKILME 240

Query: 253 KANDALRKYKMNMVIANELLTRKEEVTLVTGNENIHVRRDPIRVGDEVENHIVEHIVEKH 303
           KA  ALRKYK++ V+ANEL TRKEEV +V+ + N+ VRR+  +    VE++++  IV++H
Sbjct: 241 KATKALRKYKVHAVVANELSTRKEEVVVVSSSGNVVVRRECDKPESFVEDNLIRLIVDRH 300

BLAST of Lag0004827 vs. ExPASy Swiss-Prot
Match: Q69S81 (Phosphopantothenate--cysteine ligase 1 OS=Oryza sativa subsp. japonica OX=39947 GN=Os02g0575200 PE=2 SV=1)

HSP 1 Score: 362.5 bits (929), Expect = 5.0e-99
Identity = 186/309 (60.19%), Postives = 231/309 (74.76%), Query Frame = 0

Query: 12  ALDAEIKSFFDSAPPLKN-----------IERTESA-------RKVVCVTSGGTTVPLEQ 71
           A +   +SFF +APPL++           I R  SA       R++VCVTSGGTTVPLEQ
Sbjct: 3   AAEENPESFFAAAPPLRDADAVAARLGEFIARNSSAAGAGGGGRRIVCVTSGGTTVPLEQ 62

Query: 72  RCVRYIDNFSSGHRGAASTEYFLKAGYSVIFLYRTGTCQPYCSLLPDDPFLECFEFTHES 131
           RCVRYIDNFSSGHRGAASTEYFLKAGY+VIFL+R G+CQPYC  LPDD FL+ F+   ES
Sbjct: 63  RCVRYIDNFSSGHRGAASTEYFLKAGYAVIFLHRRGSCQPYCRFLPDDSFLKFFDVDAES 122

Query: 132 GIQVRQPYSEAVKKAISEHHAAVADGKLLKLPFTTIFEYLQMLHMIATSLRSLGPHALFY 191
            +QV + ++  VKKAI ++  A+  G LLKLPFTTIFEYLQ+L M+ATS+ S GP  +FY
Sbjct: 123 KVQVAECHAPVVKKAIGDYCKAIEGGYLLKLPFTTIFEYLQLLKMVATSISSAGPLGMFY 182

Query: 192 LAAAVSDFYVPWEGMAEHKIQSGSGPLDMRLVQVPKMLSVLRSEWAPTAYCISFKLETDV 251
           LAAAVSDFYVPW+ MA+HKIQSG GPLDMRL QVPKMLSVLR++WAP A+CISFKLETD 
Sbjct: 183 LAAAVSDFYVPWDSMAKHKIQSGGGPLDMRLSQVPKMLSVLRNQWAPLAFCISFKLETDS 242

Query: 252 KILLEKANDALRKYKMNMVIANELLTRKEEVTLVTGNENIHVRRDPIRVGDEVENHIVEH 303
            IL++KA+ AL KYKMN+V+AN L T KEEV +VT  E   +R+  +   +++E  I++ 
Sbjct: 243 DILIQKADMALNKYKMNIVVANLLATYKEEVIIVTDKERSTIRK--MNKDEDLEMQIIKI 302

BLAST of Lag0004827 vs. ExPASy Swiss-Prot
Match: Q0J7N5 (Phosphopantothenate--cysteine ligase 2 OS=Oryza sativa subsp. japonica OX=39947 GN=Os08g0176100 PE=2 SV=2)

HSP 1 Score: 345.1 bits (884), Expect = 8.2e-94
Identity = 178/304 (58.55%), Postives = 220/304 (72.37%), Query Frame = 0

Query: 16  EIKSFFDSAPPLKNIERTES------ARK-----------VVCVTSGGTTVPLEQRCVRY 75
           E +SFF +APPL++ +R         AR            VVCVTSGGTTVPLEQRCVRY
Sbjct: 11  EAESFFRAAPPLRDQDRVAGDLADFVARHSGSGGGGRLAGVVCVTSGGTTVPLEQRCVRY 70

Query: 76  IDNFSSGHRGAASTEYFLKAGYSVIFLYRTGTCQPYCSLLPDDPFLECFEFTHESGIQVR 135
           IDNFSSG RGAASTEYFLKAGY+VIF+YR G+ QPYC  LP+D FL+ FE   ES IQV 
Sbjct: 71  IDNFSSGQRGAASTEYFLKAGYAVIFIYRRGSKQPYCRFLPEDSFLDLFELGEESDIQVP 130

Query: 136 QPYSEAVKKAISEHHAAVADGKLLKLPFTTIFEYLQMLHMIATSLRSLGPHALFYLAAAV 195
           + ++  VK AI  +  A+ +G LLKLPFTTIFEYLQ+L M+ T++  LG   +FYLAAAV
Sbjct: 131 ESHAAVVKTAIRNYRKAIDEGLLLKLPFTTIFEYLQLLQMVGTAMNCLGRQGMFYLAAAV 190

Query: 196 SDFYVPWEGMAEHKIQSGSGPLDMRLVQVPKMLSVLRSEWAPTAYCISFKLETDVKILLE 255
           SDFYVPWE MA+HKI+S SGPL+M+L QVPKML +LR +WAP+A+C+SFKLETD  ILL+
Sbjct: 191 SDFYVPWESMAKHKIESASGPLNMQLNQVPKMLFILRKQWAPSAFCVSFKLETDPDILLQ 250

Query: 256 KANDALRKYKMNMVIANELLTRKEEVTLVTGNENIHVRRDPIRVGDEVENHIVEHIVEKH 303
           KA  ALRKY MN+V+ANEL   K+ V +VT N    VRR      D+VE  +++ +VE H
Sbjct: 251 KAEAALRKYGMNVVVANELANYKDVVVMVTSNGRTTVRRPSKE--DDVEEQLIDLLVEMH 310

BLAST of Lag0004827 vs. ExPASy Swiss-Prot
Match: Q9HAB8 (Phosphopantothenate--cysteine ligase OS=Homo sapiens OX=9606 GN=PPCS PE=1 SV=2)

HSP 1 Score: 219.2 bits (557), Expect = 6.8e-56
Identity = 120/273 (43.96%), Postives = 165/273 (60.44%), Query Frame = 0

Query: 37  RKVVCVTSGGTTVPLEQRCVRYIDNFSSGHRGAASTEYFLKAGYSVIFLYRTGTCQPYCS 96
           R+VV VTSGGT VPLE R VR++DNFSSG RGA S E FL AGY V+FLYR  +  PY  
Sbjct: 35  RRVVLVTSGGTKVPLEARPVRFLDNFSSGRRGATSAEAFLAAGYGVLFLYRARSAFPYAH 94

Query: 97  LLPDDPFLECFEFTHES-----GIQVRQPYSEAVKKAISEHHAAVADGKLLKLPFTTIFE 156
             P   +L     +  +      ++  +       +A+  +  A A G  L + FTT+ +
Sbjct: 95  RFPPQTWLSALRPSGPALSGLLSLEAEENALPGFAEALRSYQEAAAAGTFLAVEFTTLAD 154

Query: 157 YLQMLHMIATSLRSLGPHALFYLAAAVSDFYVPWEGMAEHKIQSGSGPLDMRLVQVPKML 216
           YL +L   A +L  LGP A+FYLAAAVSDFYVP   M EHKIQS  GPL + +  VPK+L
Sbjct: 155 YLHLLQAAAQALNPLGPSAMFYLAAAVSDFYVPVSEMPEHKIQSSGGPLQITMKMVPKLL 214

Query: 217 SVLRSEWAPTAYCISFKLETDVKILLEKANDALRKYKMNMVIANELLTRKEEVTLVT--G 276
           S L  +WAP A+ ISFKLETD  I++ +A  AL  Y+  +V+AN L +R+  V +VT   
Sbjct: 215 SPLVKDWAPKAFIISFKLETDPAIVINRARKALEIYQHQVVVANILESRQSFVFIVTKDS 274

Query: 277 NENIHVRRDPIRVGDEVENHIVEHIVEKHSAYV 303
              + +  + I  G E+E  IV+++  +H+A++
Sbjct: 275 ETKLLLSEEEIEKGVEIEEKIVDNLQSRHTAFI 307

BLAST of Lag0004827 vs. ExPASy TrEMBL
Match: A0A6J1KKG1 (phosphopantothenate--cysteine ligase 2-like OS=Cucurbita maxima OX=3661 GN=LOC111496541 PE=3 SV=1)

HSP 1 Score: 560.5 bits (1443), Expect = 4.6e-156
Identity = 284/330 (86.06%), Postives = 295/330 (89.39%), Query Frame = 0

Query: 1   MDVNGRQKAQDALDAEIKSFFDSAPPLKNIER---------------TESARKVVCVTSG 60
           MDV+G+QKAQ+ALD EIKSFFDSAPPL+NIE                T SARKVVCVTSG
Sbjct: 1   MDVHGQQKAQEALDVEIKSFFDSAPPLRNIENVSEDLKKFIEMNSSGTRSARKVVCVTSG 60

Query: 61  GTTVPLEQRCVRYIDNFSSGHRGAASTEYFLKAGYSVIFLYRTGTCQPYCSLLPDDPFLE 120
           GTTVPLEQRCVRYIDNFSSGHRGAASTEYFLKAGY VIFLYR GTCQPYCSLLPD PFLE
Sbjct: 61  GTTVPLEQRCVRYIDNFSSGHRGAASTEYFLKAGYLVIFLYRKGTCQPYCSLLPDYPFLE 120

Query: 121 CFEFTHESGIQVRQPYSEAVKKAISEHHAAVADGKLLKLPFTTIFEYLQMLHMIATSLRS 180
           CFEFTHESGIQVRQPYSEAVK AISEHHAAVADG LLKLPFTTIFEYLQ+LHM+ATSLRS
Sbjct: 121 CFEFTHESGIQVRQPYSEAVKWAISEHHAAVADGTLLKLPFTTIFEYLQILHMVATSLRS 180

Query: 181 LGPHALFYLAAAVSDFYVPWEGMAEHKIQSGSGPLDMRLVQVPKMLSVLRSEWAPTAYCI 240
           LGP+ALFYLAAAVSDFYVPWE MAEHKIQSGSGPLDMRLVQVPKMLSVLRSEWAPTAYCI
Sbjct: 181 LGPNALFYLAAAVSDFYVPWESMAEHKIQSGSGPLDMRLVQVPKMLSVLRSEWAPTAYCI 240

Query: 241 SFKLETDVKILLEKANDALRKYKMNMVIANELLTRKEEVTLVTGNENIHVRRDPIRVGDE 300
           SFKLETDVKILLEKAN AL+KYKM+MVIANELLTRKEEVTLVT NENIHVRRDP +VGDE
Sbjct: 241 SFKLETDVKILLEKANAALKKYKMHMVIANELLTRKEEVTLVTDNENIHVRRDPKQVGDE 300

Query: 301 VENHIVEHIVEKHSAYVDNFGHKASTATGS 316
           VE HI+EHIVEKHS YVDN   K S  TGS
Sbjct: 301 VEKHIIEHIVEKHSTYVDNLDQKVSRDTGS 330

BLAST of Lag0004827 vs. ExPASy TrEMBL
Match: A0A6J1GKN1 (phosphopantothenate--cysteine ligase 2-like OS=Cucurbita moschata OX=3662 GN=LOC111454754 PE=3 SV=1)

HSP 1 Score: 555.4 bits (1430), Expect = 1.5e-154
Identity = 281/330 (85.15%), Postives = 295/330 (89.39%), Query Frame = 0

Query: 1   MDVNGRQKAQDALDAEIKSFFDSAPPLKNIER---------------TESARKVVCVTSG 60
           MDV+G+QKAQ+ALD EIKSFFDSAPPL+NIE                T SARKVVCVTSG
Sbjct: 1   MDVHGQQKAQEALDVEIKSFFDSAPPLRNIENVSEDLKKFIEMNSSGTRSARKVVCVTSG 60

Query: 61  GTTVPLEQRCVRYIDNFSSGHRGAASTEYFLKAGYSVIFLYRTGTCQPYCSLLPDDPFLE 120
           GTTVPLEQ+CVRYIDNFSSGHRGAASTEYFLKAGYSVIFLYR GTCQPYCSLLPD PFLE
Sbjct: 61  GTTVPLEQKCVRYIDNFSSGHRGAASTEYFLKAGYSVIFLYRKGTCQPYCSLLPDYPFLE 120

Query: 121 CFEFTHESGIQVRQPYSEAVKKAISEHHAAVADGKLLKLPFTTIFEYLQMLHMIATSLRS 180
           CFEFTH SGIQVRQPYSEAVK AISEHHAAVADG LLKLPFTTIFEYLQ+LHM+ATSLRS
Sbjct: 121 CFEFTHGSGIQVRQPYSEAVKWAISEHHAAVADGTLLKLPFTTIFEYLQILHMVATSLRS 180

Query: 181 LGPHALFYLAAAVSDFYVPWEGMAEHKIQSGSGPLDMRLVQVPKMLSVLRSEWAPTAYCI 240
           LGP+ALFYLAAAVSDFYVPWE MAEHKIQSGSGPLDMRLVQVPKMLSVLRSEWAPTAYCI
Sbjct: 181 LGPYALFYLAAAVSDFYVPWESMAEHKIQSGSGPLDMRLVQVPKMLSVLRSEWAPTAYCI 240

Query: 241 SFKLETDVKILLEKANDALRKYKMNMVIANELLTRKEEVTLVTGNENIHVRRDPIRVGDE 300
           SFKLETDVKILLEKAN AL+KYKM+MVIANELLTRKEEVTLVT NENIHVRRDP +VG+E
Sbjct: 241 SFKLETDVKILLEKANAALKKYKMHMVIANELLTRKEEVTLVTDNENIHVRRDPKQVGNE 300

Query: 301 VENHIVEHIVEKHSAYVDNFGHKASTATGS 316
           VE HI+EHIVEKHS YVD+   K S  TGS
Sbjct: 301 VEKHIIEHIVEKHSTYVDDLDQKVSRDTGS 330

BLAST of Lag0004827 vs. ExPASy TrEMBL
Match: A0A1S3BCJ3 (phosphopantothenate--cysteine ligase 2-like OS=Cucumis melo OX=3656 GN=LOC103488424 PE=3 SV=1)

HSP 1 Score: 543.5 bits (1399), Expect = 5.8e-151
Identity = 276/332 (83.13%), Postives = 291/332 (87.65%), Query Frame = 0

Query: 1   MDVNGRQKAQDALDAEIKSFFDSAPPLKNIE-----------------RTESARKVVCVT 60
           MDVNG QKAQ+ALDAEIKSFF+SAPPL+NIE                   +SARKVVCVT
Sbjct: 1   MDVNGLQKAQEALDAEIKSFFNSAPPLRNIEDIGKDLRKFVEFNSPQAGNQSARKVVCVT 60

Query: 61  SGGTTVPLEQRCVRYIDNFSSGHRGAASTEYFLKAGYSVIFLYRTGTCQPYCSLLPDDPF 120
           SGGTTVPLEQRCVRYIDNFSSGHRGAASTEYFLKAGYSVIFLYR GT QPYCSLLPDDPF
Sbjct: 61  SGGTTVPLEQRCVRYIDNFSSGHRGAASTEYFLKAGYSVIFLYRKGTRQPYCSLLPDDPF 120

Query: 121 LECFEFTHESGIQVRQPYSEAVKKAISEHHAAVADGKLLKLPFTTIFEYLQMLHMIATSL 180
           LEC EFT ESGIQ RQPYSEAVK AISEHHAA+A+G LLKLPFTTIFEYLQMLH++A S+
Sbjct: 121 LECLEFTQESGIQARQPYSEAVKNAISEHHAAIANGTLLKLPFTTIFEYLQMLHLVAISM 180

Query: 181 RSLGPHALFYLAAAVSDFYVPWEGMAEHKIQSGSGPLDMRLVQVPKMLSVLRSEWAPTAY 240
           RS+GPHALFYLAAAVSDFYVPWE MAEHKIQSGSGPLDMRLVQVPKMLS LRSEWAP AY
Sbjct: 181 RSVGPHALFYLAAAVSDFYVPWESMAEHKIQSGSGPLDMRLVQVPKMLSGLRSEWAPMAY 240

Query: 241 CISFKLETDVKILLEKANDALRKYKMNMVIANELLTRKEEVTLVTGNENIHVRRDPIRVG 300
           CISFKLETDVKILLEKA+ ALRKYKM+MVIANELLTRKEEVTLVT NE IHVRRDP  VG
Sbjct: 241 CISFKLETDVKILLEKADAALRKYKMHMVIANELLTRKEEVTLVTTNEKIHVRRDPKLVG 300

Query: 301 DEVENHIVEHIVEKHSAYVDNFGHKASTATGS 316
           D VE HIV+HIV+KHSAYVDNF HK S A+GS
Sbjct: 301 DNVEEHIVKHIVKKHSAYVDNFDHKVSRASGS 332

BLAST of Lag0004827 vs. ExPASy TrEMBL
Match: A0A5A7VB27 (Phosphopantothenate--cysteine ligase 2-like OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold82G001160 PE=3 SV=1)

HSP 1 Score: 543.5 bits (1399), Expect = 5.8e-151
Identity = 276/332 (83.13%), Postives = 291/332 (87.65%), Query Frame = 0

Query: 1   MDVNGRQKAQDALDAEIKSFFDSAPPLKNIE-----------------RTESARKVVCVT 60
           MDVNG QKAQ+ALDAEIKSFF+SAPPL+NIE                   +SARKVVCVT
Sbjct: 1   MDVNGLQKAQEALDAEIKSFFNSAPPLRNIEDIGKDLRKFVEFNSPQAGNQSARKVVCVT 60

Query: 61  SGGTTVPLEQRCVRYIDNFSSGHRGAASTEYFLKAGYSVIFLYRTGTCQPYCSLLPDDPF 120
           SGGTTVPLEQRCVRYIDNFSSGHRGAASTEYFLKAGYSVIFLYR GT QPYCSLLPDDPF
Sbjct: 61  SGGTTVPLEQRCVRYIDNFSSGHRGAASTEYFLKAGYSVIFLYRKGTRQPYCSLLPDDPF 120

Query: 121 LECFEFTHESGIQVRQPYSEAVKKAISEHHAAVADGKLLKLPFTTIFEYLQMLHMIATSL 180
           LEC EFT ESGIQ RQPYSEAVK AISEHHAA+A+G LLKLPFTTIFEYLQMLH++A S+
Sbjct: 121 LECLEFTQESGIQARQPYSEAVKNAISEHHAAIANGTLLKLPFTTIFEYLQMLHLVAISM 180

Query: 181 RSLGPHALFYLAAAVSDFYVPWEGMAEHKIQSGSGPLDMRLVQVPKMLSVLRSEWAPTAY 240
           RS+GPHALFYLAAAVSDFYVPWE MAEHKIQSGSGPLDMRLVQVPKMLS LRSEWAP AY
Sbjct: 181 RSVGPHALFYLAAAVSDFYVPWESMAEHKIQSGSGPLDMRLVQVPKMLSGLRSEWAPMAY 240

Query: 241 CISFKLETDVKILLEKANDALRKYKMNMVIANELLTRKEEVTLVTGNENIHVRRDPIRVG 300
           CISFKLETDVKILLEKA+ ALRKYKM+MVIANELLTRKEEVTLVT NE IHVRRDP  VG
Sbjct: 241 CISFKLETDVKILLEKADAALRKYKMHMVIANELLTRKEEVTLVTTNEKIHVRRDPKLVG 300

Query: 301 DEVENHIVEHIVEKHSAYVDNFGHKASTATGS 316
           D VE HIV+HIV+KHSAYVDNF HK S A+GS
Sbjct: 301 DNVEEHIVKHIVKKHSAYVDNFDHKVSRASGS 332

BLAST of Lag0004827 vs. ExPASy TrEMBL
Match: A0A6J1BR05 (phosphopantothenate--cysteine ligase 2-like OS=Momordica charantia OX=3673 GN=LOC111004651 PE=3 SV=1)

HSP 1 Score: 535.0 bits (1377), Expect = 2.1e-148
Identity = 272/324 (83.95%), Postives = 283/324 (87.35%), Query Frame = 0

Query: 1   MDVNGRQKAQDALDAEIKSFFDSAPPLKNIER-----------------TESARKVVCVT 60
           MD NG Q  ++ LDAEIKSFFDSAPPL+NIE                  + SARKVVCVT
Sbjct: 1   MDENGPQTPEETLDAEIKSFFDSAPPLRNIENISESLKNFIEINSSPAGSRSARKVVCVT 60

Query: 61  SGGTTVPLEQRCVRYIDNFSSGHRGAASTEYFLKAGYSVIFLYRTGTCQPYCSLLPDDPF 120
           SGGTTVPLEQRCVRYIDNFSSGHRGAASTEYFLKAGYSVIFLYRT TCQPYCSLLPDDPF
Sbjct: 61  SGGTTVPLEQRCVRYIDNFSSGHRGAASTEYFLKAGYSVIFLYRTKTCQPYCSLLPDDPF 120

Query: 121 LECFEFTHESGIQVRQPYSEAVKKAISEHHAAVADGKLLKLPFTTIFEYLQMLHMIATSL 180
           LECFEFT +SGIQVRQ YSEAVK AISEHHAAVADG LLKLPFTTIFEYLQMLH+I TSL
Sbjct: 121 LECFEFTCDSGIQVRQSYSEAVKSAISEHHAAVADGTLLKLPFTTIFEYLQMLHLIGTSL 180

Query: 181 RSLGPHALFYLAAAVSDFYVPWEGMAEHKIQSGSGPLDMRLVQVPKMLSVLRSEWAPTAY 240
           RSLGPHALFYLAAAVSDFYVPWE MAEHKIQSGSGPLDMRL QVPKMLSVLRSEWAPTAY
Sbjct: 181 RSLGPHALFYLAAAVSDFYVPWESMAEHKIQSGSGPLDMRLAQVPKMLSVLRSEWAPTAY 240

Query: 241 CISFKLETDVKILLEKANDALRKYKMNMVIANELLTRKEEVTLVTGNENIHVRRDPIRVG 300
           CISFKLETDVKILLEKA+ ALRKYKM+MVIANEL TRKEEVTLVT NE IHVRRDP  VG
Sbjct: 241 CISFKLETDVKILLEKADAALRKYKMHMVIANELSTRKEEVTLVTENEKIHVRRDPKLVG 300

Query: 301 DEVENHIVEHIVEKHSAYVDNFGH 308
           DEVENHIV+HIVEKHS Y++ F H
Sbjct: 301 DEVENHIVKHIVEKHSTYINVFDH 324

BLAST of Lag0004827 vs. TAIR 10
Match: AT1G12350.1 (4-phospho-panto-thenoylcysteine synthetase )

HSP 1 Score: 395.2 bits (1014), Expect = 4.9e-110
Identity = 198/305 (64.92%), Postives = 240/305 (78.69%), Query Frame = 0

Query: 13  LDAEIKSFFDSAPPLKN-----------IERTESA----RKVVCVTSGGTTVPLEQRCVR 72
           ++ EI SFF+S+PPLKN           IE   S     R++VCVTSGGTTVPLEQRCVR
Sbjct: 8   VEDEISSFFESSPPLKNKEEIVANLNQFIELNSSCQGGMRRIVCVTSGGTTVPLEQRCVR 67

Query: 73  YIDNFSSGHRGAASTEYFLKAGYSVIFLYRTGTCQPYCSLLPDDPFLECFEFTHESGIQV 132
           YIDNFSSG+RGAASTE F+KAGY+VIFLYR GTCQPYC  LPDDPFLECFEF     IQV
Sbjct: 68  YIDNFSSGNRGAASTENFVKAGYAVIFLYRRGTCQPYCRYLPDDPFLECFEFPDAKNIQV 127

Query: 133 RQPYSEAVKKAISEHHAAVADGKLLKLPFTTIFEYLQMLHMIATSLRSLGPHALFYLAAA 192
              +S AVK A+ +  AAVA+G+LLKLPF+TI+EYLQML +IAT L+ +GP ++FYLAAA
Sbjct: 128 HGSHSGAVKMAVMDQQAAVAEGRLLKLPFSTIYEYLQMLRLIATILKDVGPCSMFYLAAA 187

Query: 193 VSDFYVPWEGMAEHKIQSGSGPLDMRLVQVPKMLSVLRSEWAPTAYCISFKLETDVKILL 252
           VSDFYVPW  M EHKI+SGSGPLD+RL QVPKMLS+LRS WAP A+CISFKLETD KILL
Sbjct: 188 VSDFYVPWLSMTEHKIESGSGPLDIRLAQVPKMLSILRSNWAPKAFCISFKLETDSKILL 247

Query: 253 EKANDALRKYKMNMVIANELLTRKEEVTLVTGNENIHVRRDPIRVGDEVENHIVEHIVEK 303
           EKA  AL+KYK++ V+ANELLTRKEEV +V+ + N+ VRRD  +    VE++++  +V++
Sbjct: 248 EKATKALQKYKVHAVVANELLTRKEEVVVVSSSGNVVVRRDSNKPESIVEDNLIRLLVDR 307

BLAST of Lag0004827 vs. TAIR 10
Match: AT5G02080.1 (DNA / pantothenate metabolism flavoprotein )

HSP 1 Score: 351.3 bits (900), Expect = 8.1e-97
Identity = 173/270 (64.07%), Postives = 212/270 (78.52%), Query Frame = 0

Query: 33  TESARKVVCVTSGGTTVPLEQRCVRYIDNFSSGHRGAASTEYFLKAGYSVIFLYRTGTCQ 92
           ++  R++VCVTSGGTTVPLEQRCVRYIDNFSSG+RGAASTE F+KAGY+VIFLYR GTCQ
Sbjct: 18  SQGGRRIVCVTSGGTTVPLEQRCVRYIDNFSSGNRGAASTENFVKAGYAVIFLYRRGTCQ 77

Query: 93  PYCSLLPDDPFLECFEFTHESGIQVRQPYSEAVKKAISEHHAAVADGKLLKLPFTTIFEY 152
           PYC  LPDDPFLECFEF+ ++ IQ                      G LLKLPF+TI+EY
Sbjct: 78  PYCRSLPDDPFLECFEFSDKTNIQ----------------------GSLLKLPFSTIYEY 137

Query: 153 LQMLHMIATSLRSLGPHALFYLAAAVSDFYVPWEGMAEHKIQSGSGPLDMRLVQVPKMLS 212
           LQML +IA +L+ +GP ++FYLAAAVSDFYVPW+ M EHKI+SGSGPLD+RL QVPKMLS
Sbjct: 138 LQMLRLIAEALKDVGPCSMFYLAAAVSDFYVPWKSMTEHKIESGSGPLDIRLAQVPKMLS 197

Query: 213 VLRSEWAPTAYCISFKLETDVKILLEKANDALRKYKMNMVIANELLTRKEEVTLVTGNEN 272
           VLRS WAP A+CISFKLETD KIL+EKA  ALRKYK++ V+ANEL TRKEEV +V+ + N
Sbjct: 198 VLRSNWAPKAFCISFKLETDSKILMEKATKALRKYKVHAVVANELSTRKEEVVVVSSSGN 257

Query: 273 IHVRRDPIRVGDEVENHIVEHIVEKHSAYV 303
           + VRR+  +    VE++++  IV++HS Y+
Sbjct: 258 VVVRRECDKPESFVEDNLIRLIVDRHSTYI 265

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
XP_023536743.11.5e-15686.36phosphopantothenate--cysteine ligase 2-like [Cucurbita pepo subsp. pepo][more]
XP_023002782.19.5e-15686.06phosphopantothenate--cysteine ligase 2-like [Cucurbita maxima][more]
XP_022952050.13.1e-15485.15phosphopantothenate--cysteine ligase 2-like [Cucurbita moschata][more]
KAG6585277.13.4e-15386.42Phosphopantothenate--cysteine ligase 1, partial [Cucurbita argyrosperma subsp. s... [more]
XP_008445369.11.2e-15083.13PREDICTED: phosphopantothenate--cysteine ligase 2-like [Cucumis melo] >XP_008445... [more]
Match NameE-valueIdentityDescription
Q8GXR56.9e-10964.92Phosphopantothenate--cysteine ligase 1 OS=Arabidopsis thaliana OX=3702 GN=PPCS1 ... [more]
Q9LZM39.0e-10963.49Phosphopantothenate--cysteine ligase 2 OS=Arabidopsis thaliana OX=3702 GN=PPCS2 ... [more]
Q69S815.0e-9960.19Phosphopantothenate--cysteine ligase 1 OS=Oryza sativa subsp. japonica OX=39947 ... [more]
Q0J7N58.2e-9458.55Phosphopantothenate--cysteine ligase 2 OS=Oryza sativa subsp. japonica OX=39947 ... [more]
Q9HAB86.8e-5643.96Phosphopantothenate--cysteine ligase OS=Homo sapiens OX=9606 GN=PPCS PE=1 SV=2[more]
Match NameE-valueIdentityDescription
A0A6J1KKG14.6e-15686.06phosphopantothenate--cysteine ligase 2-like OS=Cucurbita maxima OX=3661 GN=LOC11... [more]
A0A6J1GKN11.5e-15485.15phosphopantothenate--cysteine ligase 2-like OS=Cucurbita moschata OX=3662 GN=LOC... [more]
A0A1S3BCJ35.8e-15183.13phosphopantothenate--cysteine ligase 2-like OS=Cucumis melo OX=3656 GN=LOC103488... [more]
A0A5A7VB275.8e-15183.13Phosphopantothenate--cysteine ligase 2-like OS=Cucumis melo var. makuwa OX=11946... [more]
A0A6J1BR052.1e-14883.95phosphopantothenate--cysteine ligase 2-like OS=Momordica charantia OX=3673 GN=LO... [more]
Match NameE-valueIdentityDescription
AT1G12350.14.9e-11064.924-phospho-panto-thenoylcysteine synthetase [more]
AT5G02080.18.1e-9764.07DNA / pantothenate metabolism flavoprotein [more]
InterPro
Analysis Name: InterPro Annotations of Sponge gourd (AG-4) v1
Date Performed: 2022-08-01
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
NoneNo IPR availableCOILSCoilCoilcoord: 237..257
NoneNo IPR availablePANTHERPTHR12290:SF20PHOSPHOPANTOTHENATE--CYSTEINE LIGASE 2coord: 14..305
NoneNo IPR availablePANTHERPTHR12290CORNICHON-RELATEDcoord: 14..305
IPR007085DNA/pantothenate metabolism flavoprotein, C-terminalPFAMPF04127DFPcoord: 153..261
e-value: 2.5E-12
score: 47.0
coord: 37..88
e-value: 1.5E-5
score: 24.9
IPR035929CoaB-like superfamilyGENE3D3.40.50.10300coord: 28..305
e-value: 5.6E-102
score: 342.9
IPR035929CoaB-like superfamilySUPERFAMILY102645CoaB-likecoord: 35..301

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Lag0004827.1Lag0004827.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0015937 coenzyme A biosynthetic process
cellular_component GO:0005737 cytoplasm
cellular_component GO:0005634 nucleus
molecular_function GO:0004632 phosphopantothenate--cysteine ligase activity