Lag0004695 (gene) Sponge gourd (AG‐4) v1

Overview
NameLag0004695
Typegene
OrganismLuffa acutangula (Sponge gourd (AG‐4) v1)
DescriptionPeptidase_M3 domain-containing protein
Locationchr6: 6165045 .. 6177676 (-)
RNA-Seq ExpressionLag0004695
SyntenyLag0004695
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: polypeptideexonCDS
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGACAGAGATTCAAGGAAACAACAGTGAGAAAATGGACAAACGGAAGAGCCAAGGTAAGCTGCTAGTCTTCACCGGAGGCGCTGCTCTTCTCGCCGTCGCCGTGAACCTCGCAATCGTCGCTATCAGCAGACGGAAGAAGAAGAAAGGTACTTCACACACTTCCATTTCGCTTTCTCACTTCACTTTCGAGCCGTCTTGCATTTCAGTGCTCGCTGTAACACATCCGTGCATGTTAAAATACTCGCATCTGGCATGTACATGCAGTGAAGATTGCGTCGGTAGCATTTTCGTACTGTTGATTTGATTGTTTTTGATGTACAGACCTTCCAGGATTTGAGCTACGCGTCAACCTTTCGCCATCTGAGATTCTGAATTTAGCGGACAGAATTATTGCGAAGTCGAAGAAGGTTCATGATGCTGTTGCGTCGGTTCCTCCTAACAAGGTGCCGGCTATCTTGCTTTTTACTTGCTAACTTCTGTATCTATCTGTACTCGCTTCTTTTCATTTTTTTTATCCATTATGCCAAGACACGCATCTGCAATTACGATCAGTTCGGACAGTGAAGGATGTGGATCGGTTAATCCAATAATGTTCTTGATAACTAGTTATGACTCAGTGGAAGTCTGGACGCATAATCAGTGGTCCACTGTCATGGAATTAGTTTTACATATGCGATCAACAGATGACAGTATAAATGCTGCCACTTCGATTTGGAATTTGGTCAACGATAATTTGGAAGTAAACATAAGAATAAGGCCGCATGTGTTTAGTCTTATATCACAAATCCATATCTTCCTTACAACCTGAATTTGATTTCCGATATGTTCGTAGTTCGTCTTTATAATACCTCAACTGAAGCTTGCAGGTTACATATTCAAATGTAATTTCTCCGCTAGCTGATTTAGAGGCTGAACAATTTCCGCTAGTACAATCATGCGTGTTTCCAAAACTAATATCTACTTCAGATGATGTGCGCAAAGCAAGTGCTGAAGCAGAGCGGAGAATAGATGCTCATGTTCAGATGTGCAGGTATCAACAATTTCTATCTTTTCTTGAAAGGGTACATTCATTAGCTTTCGTAGATTATGCAGCAGGGTACTCTTGAAGGCTTAAACTGATTGCACCTTGTTTCTCCCCCCACTACAGGCATATCCATGTTTCAAAAAGATGCATATTGTTTGCCACAATAGGGAGGAGAAACAAAATGAAGCGAATTATGTACCTGGATTCTTAATTAATGCTATAGGAATTAATTTTGTAATTTTTTTTGAACAAAAAATAACGAATAAATTTTGTAAAATTGAAGGCTTAGAAACATGTCCCTGCCAAACCATTCTTTCTAAATTTTATTTTATTGCAGCGTGAGCAAACGTCAGAGCATGATAGAATTGCATGCCCTTGTCAGTTAAAGAACTCATTGTTTTTTCTCTGTGTGCTGAACAGCAAACGTGAAAATGTGTATCGTGTTGTCAAAGCTTTTTCTACTAGAGGGGAGCAGACAAGTGCTGAACAGAAGTGTTTTATCCAATGCTTGGTATGAACTCATAGTAGGTCGTAATATAGGGAGATCCTTATCCTCTCTTTTCCTGAATACAATGATATGTACATTCTCTTCAAATAGGAAGGATAACTACTAAGATAATTGCATAGAATGGAATGTAGTCTATAATTTAACTTATAAAGAACCAAGATGGCAAGACTCTAATTGGGCATTGAGCTTAAAAACAGTTTGTCTTAAAAAATTGTTCTATGTTGCCAATTTGTCCGTGGGTGATCAAGGCACATATACCTTCCCTAAGGTTGAAGGTTCAATTCCCGCCCCCACATTTGTTGAACTAAAAAGATCCCCCCCCCCCCCCCCCCCCATATTCAGATCTTCGTTCTTTGGAACTTCTTGTTCTGTACTTTTTCCAAGGAAAGGATGACTGATTTTGTGAACAACAGAAGTTGTTATTCCTTAAAAATATAGATTGATCACAAATGTAATGCAACATCACTTTTAAAAAGAACCTGATCAATTTTAAAAACAGCTCTGTAGTTTTGGTTGAAAACTTTCCAAACTGCTCCTCATAGTTCAAGAGATAAGGAGTAAGGACATTGATTTCATTGAGGCAAGAGTATCGGATTTTGCATATGTTGGTTTTATGATCCACATTCTTACAATCTAATCACCAGAATCTGCAACTGAAATATTAGAAAGAATAAACTACCTTAGTCATTTTTTGTTCTTTTTGACGAGATTTGAACTTTACTCTGCTCCAATTGCTTAAAACCTGTGGTGATTCTTTTCAATCAGTGACAGGTGAGAGACTTTGAGCGAAATGGCTTAAACCTTACTTCAACAAAGAGGGAGGAATTGCTGCGACTGAGGGTGCAAATTGAAGAGCTAAGCCTGCGATATATCCAAAATCTGAACGATGATGGTACATTCCTTCCGCTTAGTGAGGCTGAATTGGATGGTTTACCAAAAGAATTTTTTGAGGTTTAGGTTCTTCTTCTCGTATTATATATTTTAAGTGTATATTACGTTAATGCACTCACACAAATTAGATACCGACCTATAAAAATCCTTTTTGTTAGCTTTTCTTTCTGGTGGCATCATATCACCTTTTCCTTTTGTTTAATTCACCTGTATATTTCTGAAAAGTCTTTACTCATCATAGCTCAAGATACTTTGATTGTACATGTAGATCTGACTGGATGTTCCAAGTTATTTCTGAGCTATATGATATGCTTTATTATTCAAACTTCAATATGTCCACATTAGTGATATTAGTGATATGTTGTTTTATATGGTATAATAAAAATGTCAATATGTCGATGTGGGATCAATGGACATGTAGAAATGTTGGTGGAAATTTAATAAGGATGATGTTGGCAATGAAGTAAATGGAAGTTATAAACAAAGATTTCTGTGGATCAGTGTTTGTGAATAAGTGCAGTGTTACCAAAATTTTGAAGGATTTGATCACTCTGATTTGTGGACCAATGATTTAACCTTGCTAATATGAAATCTACTTTATAAACTGGGAACTACTCTGATTTATCAAAATAACTTTTTGATTGATTTATTCCCACATGCCATCGACTAATGTACTCACTGTATCTCTTCTCCATCCAGAGTCTTGATAAAACTGAAAATGGTAAATTTAAGGTGATCTTGAGAAGCCATCACATTGCGGCAGTATTAGAACATTGCAAGGTATGCTCTTCTGAACAAAAAGCTATTAGGAATATCGCCCATCTAGTTCAAATTCCGACTCAACCTATTTGTATCTTGTATCTTTCTTTTTCCAAATCTAATAATGCCTTAATCAGATTCAGTCCTTCAGGTTATCTTATTTTGTTTCTTTCATTTTTATTCTGTAATAATTTCTATCTTGGCATTTTATCTTATATTGTATCTGTTCTCAATTTGAACTACTCCACAGAGTTAGTTATCCATGTAACCTTCGAGATCTTAATACACATATTATTGTCTATATTTAATTTGTAAACTTTTAATGGAAGGAAGAAGTAAAGTTTGGGAGCTTTTAGTCCTTAGTGAACCTGATGCTGGAATTCTTTGGAAATAGTTACCCTAGAAAGTTAACTATTAGTTTTAGGCATTTTGCATAATATATTGAAAATTGAGAAAAATCCAGAGCTCAAACAAAATATAGGTCAACTAGAAAAATTAGGGCAAACATCTAGCTTTAACTGAAAGTCATATCATGCAACTTTCTGCATACAAACTCAATGACTGAAGTTTTTGAGTTGGGTATTATTTGTGAAGTTCTTACCATAGATTCAAAGGAGAAATATGTACTGACAATATCAACACTGCCTGATTTAAGGTAGGCACGACAAGGAGGATGGTGGCAATGGCATATGGAAAGAGGTGTGGAGAAGTGAATCTTTCTATTCTAGAAAATTTGGTAATTAACCTGATTATCTCTTCTTTATGAACTAATTTAAACTTCAAATAGGTTACTTGGTTCTGAGCTTTGCGTGACCCATTAACTTTTGTATTTAGATTATAAGGGAAGCCATCATTTTACTTTCTCATCCAATTCTTAAAATGGTTTTATATGGATAATCTCTTTTTTTAATATTAAAAATTGAACCTTGCACCTTATCCTTCCTATTAACGTATTACATGTATAGATTAGAGGGCAAGCTGTCGTGATTTCTATATCCATTTTTTTTTCTTTTTATTGGTTTTAGTGAGCAAGTTTTGATTTGGTGGTCCATAATCTACTTTGACAAATGATATTTCCTGTTTCTAAAGACTATGTTTATGAATAATAATATTCCTATTTCCTTAGATTTTTCACGTTTGTTGTAGCGATATGATCAATATGAAAGAAGTAAATATTGTTAAAAGATGGAAAATTGACAATTCTAAATAATGTAAAATAACATGACAATGGTTCTTTATGCTAATTTATGTTCCACAAAAAGTTTTTCACATTCCAAATGCCTTCCACTATGAACAGATCTTGCTTATGTGGAAGTTTCTAGAAAGTTGCGGTTTACCATTCTTTCTAAATTTTCAGGTTCTTCTGCGCCACAAATTTGCTCGTTTACAAGGCTATTCAAACTATGCGGACTATGCTGTTCATTATAGAATGGCAAGGTCGTCCTCTAAGGTTGTTCAGAGATGCCAGTTCTTTTCGTTGATCTAATATTGTTATGACTTTTTAAAAAATAGGAAGTTAAAATGGGATTAAAAGAGTTTATTTTTTTTTAGCCATCACTTCACTTTTAGGTTCTGATTGAACCGTAACATATGAAGAACTACCAACTAGTTTAGTTAATTCTCAATTTAATGTCAATTTATTTGTCAACTTCTAGGCTGGTAATTACTTGAATGTTTATTCAGGTATTTGAATTCTTAGAGAACATATCTGATAGTTTAACAGATTTGGCTGCCAAGGAACTAGCCAGCTTGAAGGACTTAAAGGTTAGCTCATTCAGTCAAATGTTTTTCCATGACACGGTTTCATGTAACTTACTTGGGTCTTTTGAGACATCAGAAGCAAGAGGAAGGAGAAAGTCCATTTGGCATTGAGGATTTATTATACTATGTCAAAAAGGTTGAGGATCAAGAATTGAACCTGGACTTTATGACTCTTAAGCAATACTTTCCAGTCTCTCTGGTTTTATCTGGGATCTTCAAGATAATGCAAGACCTCTTCGGTAATCATTTCCTTTGCAAAAATTTTGAAGGGCATGATCCTACCGCCTTTACTCATGAATTTAATTTTTCTTTTTGCCTTAAAAACTTAGTACTACAATTGCAATTTTGCATTTGAATTTTTGCTTCATCAGGTTTAAGGTTTGAGGAAGTGATAGACGCTGAGGTTTGGCATTATGACGTCAAGTTATACTCAGTTTTTGATCTCAACTCTGGTGAACTAATTGGCTACTTCTTCCTTGATCTATACACAAGGTTTGATCGGGACTTCAAACTTTTTATAAAATTGACATTGCCATTATTTCTTCATCGGTGGGTGAAATTATGTTTTGTACTTGGTTAGGGAGGGAAAATACATTCACACGTGTGTGGTGGCCCTTCAAAATAGTGCATTGCTATACAATGGTACACGGCAGGTAATATTTGTAATATACTTAAAATGTTAATGCTGAATTATTGTGGTTTTTTAACTGTTCTTGAGGTTTCTCCCCTTTTTGTTATTTATTATTTATTTTATGTGGGCATTCTCTTGTTTATGAATTAACAATACTTGGGCTCAATTCCCTTTCTCAAACAGGTGCATTTGATAATTTGATAAGAAACAGAAAATCAATTTGATTTCATATTTTAAAAAATTATATGAAATTAGTTTTTGGGGTTTTTGTTAGCAGGTTTGTATTTAGTTTTTGAGGCGTGTTTTTTATATGCCCTCGTTCTCCCTTTCATTTTTTTTTTTTTTAATGAAAGTTCGGTTTTACACATACATACATACATACTAAAAAATCATCCATTTAAAGAAATAAGTCACTGAAACACATAATTGTTATCGAGGACACTTTTTCCTTCCTAATTTGGTATATAAATTGGAAAATAAATAATTATTTAATGCCTTCTAGCTCTCTTTTCTTTCTTTTTTTTTTTTTACAATATGTGGGATAGGGGATTCAAACCATAGACATGTTGGTCATCAATACAACACTATGCCAATTGAGCCATGCTCTTGTTGGCTTCTAGATCTCTTTTCAAAATAATGGAAACATGAATAAAATATAAATGATAAGAAATGGGAACTGGACTCCTAATTGAATTGGACCTTAATGACCAGAATCTGATTTGCATCTGCATCATGAATTCATTTTGCCTTTTTTTCTGCCAGGCAACTATTTGTCATTGACAAGGAAGAAATATATTAGAAATCCAATGTTTATTGTCACTTACATGATACATGTGGTATCATATACCTAGAATGTATGTTTATTGGTTATTATGATAATCTCATATACCTAGAAACCCAATGTTTATTGTCACTTACATGATACACGTGTGCAACATTTATGAAGAAAATTGTACTTGGAACAAGTTGAGACGTTTAGTTCTTTGTATGATAACTCAATAGACAAATTGTATCCTCATAAACTATCTTTTTTTTCTTTGATTAGATACCAGTGGCATTACTGATATCTCAGTTAGAGAAGGATGTTGGAGGTCATGCTGGGTTGATGCGATTCACTGAAGTGGTTAATCTCTTCCACGAGTTTGGCCATGTGGTATGATATATTTTTTATACTTGCTTTCAGACCAAGACCAATTTGATCAAACAATTAGAACTTAGAAGATGATAATTTATTGTGGTTTGACACTGGAAGATGGTCAGAATTAAATTGAGGCAAGTTCCTTAAGTTCTAGAATCTGATTTATGATAAATATGTATTGTGGTCATTGGGCTTGGCCATGCCCCTTAACTGGAACAAGACCTCCCTTGCTCGATTGCACCTACATGTTAGACAGAAAAGATTTATCATAAATGTGGTGAGCAAAATCTTGATGGAAGTTATAATGTCTGCTTGTGGAGGCATGATGGCATCCAAACTAAATGATTACCACTACGGTCTTATGTGGCTCTGGGATAAACTACCTTAACAATTCATTTCAGAGTTTCAGTGCTTGTCCAACCATCCTTTTATATTAGTTTGCTTGCAATATTGCTATATGTTAAATCACCACTCGACCAAAAAGCTTATGCTGATGGGTTACGATAAATTCAATTATATCTACACTTTTTTTTAAAGAAGAAACGAAGATATCATTCACAAAAGCCAAGCCAAAACAACAACCTAAGGACAGAGGGATAAGGCATCCCTCATCCAAAAGAAGCTACAAAAAAGCCTTCCATTGAAGGTTGATCATGGCAGCAAAATAATTACAAAAAATTCTATCTTGCACACTCCAATATCTACACTTTAACACTCCCACCCACTTGTAGGCTTAGAAATTTGTAGAAGGACCAACAAGTGGAATTCAATATTAATTGGGGAGGAAATGACATTATAAAGGTTCGAACCTAGGATCTCTTGCTCTGATACCATGTTAAATCACCACTCAACCTGAAAACTTAAACAGATGGGATGAGACAGATTTAATAATATCTATACTTTAACACCATGTTACATTAGTCTTTCTGTTAGTATTAATTGAAAACTAACATCAGAGGTAAAAGGTACAGTTTTCTAAATCTTAAGCATTTCATATTTCTGTGATTTTAATGTATTTTCCTCAACTTGACTGAAATTTTAGGTTCAACATATTTGCAATCGTGCATCATTTACTAGGATCTCAGGGCTGCGTGTGGATCCTGACTTTGTTGAGATCCCTGCTCAGATCCTAGAAAACTGGTAGCTGATGAATTGTAATATTCCTTAGTTTTATTTGACAACTGCATCCACCTATTAATACTCGTGTTTGTAGGTGCTATGAAAGTGTTTCTTTGAAGTTGTTATCAGGGTTCCATCAGGTAATGCATTTATATTAACACTTCCTTTTAGTTTCATTATTTGCAAGTCTGGTGCATCTCAATGTATTCAGCGCTAATGTTGGAAAAACCTCGTAATGACTGATCAAAAAATATTTGGTTTAAAGCTTTAAATTTCTGTTGTTCTGATAAGTTGGTCAAGGCCATTTGTGATGGTTATATTTTACCTGAGTTACGTTTTATAGTATTTATCATTGTGCGTATGAAGCCATATTTTCTGTTTGTGCAATGTTTAGGATATTACAGTGCCCATCAAGGATGAAGTATGTGAATCACTGAAAAAATGGCGACATTCTTTTTCTGCGCTGAAATTGAAGCAAGAAGTTCTTTATTGTATGACATTTCTTGTACTCTGTCTACCTCCTCCTTCTTCCCTTCCTTTTTTTGGGTGAAACAAGAAACATCTTTTCATCTTTTCATTGAATGATCCAAAGTTACAGTAATAGACATGCAACATCTCCAACGGGGTTTACATTTATGCACCCCATCATTAGATTAGGAGAACAAGAACAAAAAGAGCTTGATAAAGCACTCCGAGAAGAAGCAAAGATTTTTTGCCAAGTGCATTTTCCTTCAAATATTATTTGGTGCCTTTTGGTAAATTATTCGGAATCTTTCACACTAAAGACCCCAAAGACTCAAAATGATTGCTTTGACAGCATTGACCCAAAGAAATTTAGCCCTGTTTTTTAATCTATGACCGCCAACAAACTAACTAATGCTGTCCTTCGGAGATGAATCAAAAGCACAAGAACAACCAAAGCGCAAAAAGACAAACTCCCTGCCTTAATGATTGGCACACAACAATTAAGACCTGGAACATTATTATGTCATTTTTTGTACTCTGTTGCCCATATGAAGAGAACCCAATTAAGAATCCAATCCAAAACATTTACTTTCTTTGGACCTTTTGTTTTTCATAGTTCTTTCTAGAGATCTTTGAGCTCAAAGGGGAACGATACTATTTCCTTAAATAGGAACTTCATTGTGGAGCAACCTGAAGGGTCCAATGTCCATAGCTTTTTGTCTTTCCTCCTTCCAATAGCAAAATCTTTGATCAGAATTAGCAAAGTGCTTAACTCTTCCATTTTTTTCCTCATGTAAATTTCTTCTAGCAACAACATTTCAAGAGTGGGTTTTCTTGTCTCAAACGTCATAATGTTAAAGCATTTCTCTGCTTTTAGATCACATAAAACCCAGGAAATTTTAACAAAAGAGTTTGGTTTCCCCAAATGTCATTCCAAAGGAAGGATTAGCATCCTCCCATCTCTAACGCTAATGGTTAATAAATTCTGCGTCACATTAAAATTTTGATATGTTCCTTAAGTGTATCATGCTGTCTGATTTCTTATTTTCCGTAGTCCATGCATACTCATACTTTTCTTTCTTTCTTTTTTTTTTTTTTTTGAAAAGTTTACTCATACTTTTCAAGAATACATCACACCTGGTGCAACAACTCATGTGTTTCTTTTGGACTTCTTAGCAATCATTTTGTCAAATAAGGCTTCATTATTTCTTTGAATTCCCAATACCCAAGCTTCCACATTCAAGAGGTTTGGTCAGATCACAATATGCCAACTGATCAAATGCCTCAATTTCCCTTCATTATTTCCTTTAGAAACGAAGTTCCTTATTTTTCTAAGTTTGAGCTATCTTCCTTGGTAAAAGAAACAAGGACGTATAATAGGTTGAAAGACTTGTATCACAAAGTGTCCATATCTATTACCACCTCCTAAAGGGCATCCTCAGTAAGATAATAGACCTTCATATTTGCATCCCAATGTGTGAGCCACATTTTTCTTGCACTCTATCCATCAGATGCGTTCTTGTTCATTGTATAACTTGGGAAATGTCTGTATGTAGGTCTTTTTGATCAAATTATACATTGTGCTGAAAATGTTGACATTGTTGAATTATTCAAGCATCTCCATTCAAAGGTAATATTTTAAAGTATTTATTTCTTAACTTGAGTTTTGCATGCTAGGTTTTCATGTAACGGCATTTTCCCAGGTAATGCTAGGTTTGCCCATGCTAGAAGGAACCAATCCTGCTTCATGCTTTCCATGTAGTGCTATCGGTTATGAAGCTGCCTGCTACAGTCGTGTATGGAGTGAGGTATATATTGCTCTTTAAGCACGTAAATTGTTTTTGGAATATGCTTACTCATAAATCTACGCTTAACATGTTGTGTTTTTCGGAAATTTTTAGTAGTTCACTAATGTAATAATTGGATCATAGTGCAAAAATGGAAAGGCGAGGTGTTCATGAGCATTTCTGTACTGAGCATTGTCATCTTTGTTTAAATAAAACATGAGCAGCAACCTAACTGTTGCAGGTTTTTTCGGCCGATATTTTTGTCTCAAAGTTCCGCGGCGATCTTCTGAACCAGCATACTGGCTTGCAGTTCAGAAACAAGGTAATAAATTTGCATTATGCATCAAACTTTGATGGTATTTTGGACCAATTTTATGTCACCATTCAATTCAGTTTAGTCAAAGCAATGTTACTAGAGTCAAATTATCTCACATTCATTTGGTACCCAATTCAAATTTGGCTGCCGTGCCTGTTTTTTGCTCGTCTTCCCAAATCCATAGTAAGATCTAGATGTTTCTATTTACATGTCTATTTGACAGATCTAAATTGATATTTGTATTAAAATAAGAAAAATATTATAAAATAGGGGAGAAATCCAAGAAGCCTCTTCTACTCCCAATTCCAAACTATTTGTAATACCCTGCCCATTATTAACCTATCTTACCTCCCCTCTCTTTTTATAATCACCAGCAATTAACTCCGTTTAATAACTCTTAATAAAATCTAAATCCCCTTGTAACTAACTCAATATTCTTACTATATCCAAACACCTAACATTACTTACCGTGCAAGACGACCAACCTTCACAATGCTGGAAACATTGCTAAGATATTATACTGCCTGGCCTTACCCGACCTAAAGATTGGTGAATTCTCATAAACCTTGGTTATAGCCAATTGACGAACTTCCTTAAAAATAGTATTCATTGGCATTTGGCAGACACATTTGAAGGTTGATTTGGCCATAGTTTATCACTGAAGTATACCCCAAACATTGCTAAAAAATTTTGTGAGTGGGTAGTACTTTAGAGTGAAATATCAGAAACCCAAATAAATGCTGGGCTAGGCCAAAAGATCCGAAACTTAACTCAGCTTCCCACACCCAATATTCTGGCCTTCTACATGAAGTTTTAGATTAAATAGAGTCCTGATTATATTCTCTGTACTCCAGAATGTATTATTATCTCAAACACCAATTGTTCCTCCATTAACCTCCTGAGTATTCATCCACCACTAATATACTGGTTTTTATGAGGGTCTGTAATCCATGTAATGGACTAAGCAACAATAGCCAATGGCATTGGCAAAATATGTGTTTGCGTGGAAAGGAAGGCCTTCAATTAAGAGTACTCAGCAAATATTTCAAATGCAAATTTTGTAGCTAATTTGGCCAATAATGGAACAATTCCACCAATCATCAGATTTCCAAGGTAGAGTATGAGCTTGTTTTGCATGACTTTTCAAGTGCTTAAAAAGGAGTATTTTAAGTGTAAGAATTTTTAAGTACTTGGAAAGTCAAGTCCTACATCTAGTACATCTTCACCAACCTCTTTAAGCCTATTACCATCACTTTGTTGTCACTTCCATCACTTTATGCTAAAGGCCCACTTGGTCATACCCTTTTGCCTTGAGCAATCTCCATGCACCTAAGCCATAACCTTTGCGATTTTGTTTATAATAGTCGGCCATTAATTCCCTAATAACTCTGACATATTCTTACTGTCCCATATAACTAACTCATACAAACTAGTTGATATGCTAACCATCATCGGTTCACCTAGTGGTCAATAAGAACCATGTAAATAATAAAGGTTCATAGGAAATGAGTTCAAGTTATGGTGGTCACCTACCTATGATTTAACATCTTATGGGTTACCTTGGCAACCAAATATAATAGAGTTAGACGATTGTCCCGTGAGAATGGTTGTGGTGTGTGCAAACTTGCCCAAACACTCACATATATCCAAAAAGGAAAAGAAAAAGAAAAAAACTAACTAGTTGTGTTATGACGAAAGCAGTAATCAACGTAACAACAAGGAAAGGGCGAAGAGAAACAACACACAAATATACATGGTTCACTAACGGTGTGTTAGCTACATCCACGGGCAGAGGGAGAGAACAATCTTATTATGAGAGGAGTATTCAGAATACAAAGATTTAGGGAAAAAATAGGTTAAGAGGGTTTATATATGGCACCACTTTTAACCCTAACATAATAGGCCCAAAATAAAAGTCCAAAACAGAAATCATATCTAATCTCTATACATAATTTAGCTTCGTACTTAGTCGTTCGGAGTGCTTCATATCAGATTCAAGGCATATCAACAAGTTGATATGCTAAATAGTACTGCATTGTCTAACACACTATCTTAAAGACCTATACACGGTATCATCCAGAAGGGCTGCTGAATGGCCTTTTACCTATCTAATGTGGCAGGTTTTGGCTCCGGGAGGTGCAAAAGAACCAATTGATATTCTCTCAGATTTTCTTGGGAGAGAACCATCAATTCAAGCTTTTATTGACACTAAGGCTGAATATAGCTTGTGA

mRNA sequence

ATGACAGAGATTCAAGGAAACAACAGTGAGAAAATGGACAAACGGAAGAGCCAAGGTAAGCTGCTAGTCTTCACCGGAGGCGCTGCTCTTCTCGCCGTCGCCGTGAACCTCGCAATCGTCGCTATCAGCAGACGGAAGAAGAAGAAAGACCTTCCAGGATTTGAGCTACGCGTCAACCTTTCGCCATCTGAGATTCTGAATTTAGCGGACAGAATTATTGCGAAGTCGAAGAAGGTTCATGATGCTGTTGCGTCGGTTCCTCCTAACAAGGTTACATATTCAAATGTAATTTCTCCGCTAGCTGATTTAGAGGCTGAACAATTTCCGCTAGTACAATCATGCGTGTTTCCAAAACTAATATCTACTTCAGATGATGTGCGCAAAGCAAGTGCTGAAGCAGAGCGGAGAATAGATGCTCATGTTCAGATGTGCAGCAAACGTGAAAATGTGTATCGTGTTGTCAAAGCTTTTTCTACTAGAGGGGAGCAGACAAGTGCTGAACAGAAGTGTTTTATCCAATGCTTGGTGAGAGACTTTGAGCGAAATGGCTTAAACCTTACTTCAACAAAGAGGGAGGAATTGCTGCGACTGAGGGTGCAAATTGAAGAGCTAAGCCTGCGATATATCCAAAATCTGAACGATGATGGTACATTCCTTCCGCTTAGTGAGGCTGAATTGGATGGTTTACCAAAAGAATTTTTTGAGAGTCTTGATAAAACTGAAAATGGTAAATTTAAGGTGATCTTGAGAAGCCATCACATTGCGGCAGTATTAGAACATTGCAAGGTAGGCACGACAAGGAGGATGGTGGCAATGGCATATGGAAAGAGGTGTGGAGAAGTGAATCTTTCTATTCTAGAAAATTTGGTTCTTCTGCGCCACAAATTTGCTCGTTTACAAGGCTATTCAAACTATGCGGACTATGCTGTTCATTATAGAATGGCAAGGTCGTCCTCTAAGGTATTTGAATTCTTAGAGAACATATCTGATAGTTTAACAGATTTGGCTGCCAAGGAACTAGCCAGCTTGAAGGACTTAAAGAAGCAAGAGGAAGGAGAAAGTCCATTTGGCATTGAGGATTTATTATACTATGTCAAAAAGGTTGAGGATCAAGAATTGAACCTGGACTTTATGACTCTTAAGCAATACTTTCCAGTCTCTCTGGTTTTATCTGGGATCTTCAAGATAATGCAAGACCTCTTCGGTTTAAGGTTTGAGGAAGTGATAGACGCTGAGGTTTGGCATTATGACGTCAAGTTATACTCAGTTTTTGATCTCAACTCTGGTGAACTAATTGGCTACTTCTTCCTTGATCTATACACAAGGGAGGGAAAATACATTCACACGTGTGTGGTGGCCCTTCAAAATAGTGCATTGCTATACAATGGTACACGGCAGATACCAGTGGCATTACTGATATCTCAGTTAGAGAAGGATGTTGGAGGTCATGCTGGGTTGATGCGATTCACTGAAGTGGTTAATCTCTTCCACGAGTTTGGCCATGTGGTTCAACATATTTGCAATCGTGCATCATTTACTAGGATCTCAGGGCTGCGTGTGGATCCTGACTTTGTTGAGATCCCTGCTCAGATCCTAGAAAACTGGTGCTATGAAAGTGTTTCTTTGAAGTTGTTATCAGGGTTCCATCAGGATATTACAGTGCCCATCAAGGATGAAGTATGTGAATCACTGAAAAAATGGCGACATTCTTTTTCTGCGCTGAAATTGAAGCAAGAAGTTCTTTATTGTCTTTTTGATCAAATTATACATTGTGCTGAAAATGTTGACATTGTTGAATTATTCAAGCATCTCCATTCAAAGGTAATGCTAGGTTTGCCCATGCTAGAAGGAACCAATCCTGCTTCATGCTTTCCATGTAGTGCTATCGGTTATGAAGCTGCCTGCTACAGTCGTGTATGGAGTGAGGTTTTTTCGGCCGATATTTTTGTCTCAAAGTTCCGCGGCGATCTTCTGAACCAGCATACTGGCTTGCAGTTCAGAAACAAGGTTTTGGCTCCGGGAGGTGCAAAAGAACCAATTGATATTCTCTCAGATTTTCTTGGGAGAGAACCATCAATTCAAGCTTTTATTGACACTAAGGCTGAATATAGCTTGTGA

Coding sequence (CDS)

ATGACAGAGATTCAAGGAAACAACAGTGAGAAAATGGACAAACGGAAGAGCCAAGGTAAGCTGCTAGTCTTCACCGGAGGCGCTGCTCTTCTCGCCGTCGCCGTGAACCTCGCAATCGTCGCTATCAGCAGACGGAAGAAGAAGAAAGACCTTCCAGGATTTGAGCTACGCGTCAACCTTTCGCCATCTGAGATTCTGAATTTAGCGGACAGAATTATTGCGAAGTCGAAGAAGGTTCATGATGCTGTTGCGTCGGTTCCTCCTAACAAGGTTACATATTCAAATGTAATTTCTCCGCTAGCTGATTTAGAGGCTGAACAATTTCCGCTAGTACAATCATGCGTGTTTCCAAAACTAATATCTACTTCAGATGATGTGCGCAAAGCAAGTGCTGAAGCAGAGCGGAGAATAGATGCTCATGTTCAGATGTGCAGCAAACGTGAAAATGTGTATCGTGTTGTCAAAGCTTTTTCTACTAGAGGGGAGCAGACAAGTGCTGAACAGAAGTGTTTTATCCAATGCTTGGTGAGAGACTTTGAGCGAAATGGCTTAAACCTTACTTCAACAAAGAGGGAGGAATTGCTGCGACTGAGGGTGCAAATTGAAGAGCTAAGCCTGCGATATATCCAAAATCTGAACGATGATGGTACATTCCTTCCGCTTAGTGAGGCTGAATTGGATGGTTTACCAAAAGAATTTTTTGAGAGTCTTGATAAAACTGAAAATGGTAAATTTAAGGTGATCTTGAGAAGCCATCACATTGCGGCAGTATTAGAACATTGCAAGGTAGGCACGACAAGGAGGATGGTGGCAATGGCATATGGAAAGAGGTGTGGAGAAGTGAATCTTTCTATTCTAGAAAATTTGGTTCTTCTGCGCCACAAATTTGCTCGTTTACAAGGCTATTCAAACTATGCGGACTATGCTGTTCATTATAGAATGGCAAGGTCGTCCTCTAAGGTATTTGAATTCTTAGAGAACATATCTGATAGTTTAACAGATTTGGCTGCCAAGGAACTAGCCAGCTTGAAGGACTTAAAGAAGCAAGAGGAAGGAGAAAGTCCATTTGGCATTGAGGATTTATTATACTATGTCAAAAAGGTTGAGGATCAAGAATTGAACCTGGACTTTATGACTCTTAAGCAATACTTTCCAGTCTCTCTGGTTTTATCTGGGATCTTCAAGATAATGCAAGACCTCTTCGGTTTAAGGTTTGAGGAAGTGATAGACGCTGAGGTTTGGCATTATGACGTCAAGTTATACTCAGTTTTTGATCTCAACTCTGGTGAACTAATTGGCTACTTCTTCCTTGATCTATACACAAGGGAGGGAAAATACATTCACACGTGTGTGGTGGCCCTTCAAAATAGTGCATTGCTATACAATGGTACACGGCAGATACCAGTGGCATTACTGATATCTCAGTTAGAGAAGGATGTTGGAGGTCATGCTGGGTTGATGCGATTCACTGAAGTGGTTAATCTCTTCCACGAGTTTGGCCATGTGGTTCAACATATTTGCAATCGTGCATCATTTACTAGGATCTCAGGGCTGCGTGTGGATCCTGACTTTGTTGAGATCCCTGCTCAGATCCTAGAAAACTGGTGCTATGAAAGTGTTTCTTTGAAGTTGTTATCAGGGTTCCATCAGGATATTACAGTGCCCATCAAGGATGAAGTATGTGAATCACTGAAAAAATGGCGACATTCTTTTTCTGCGCTGAAATTGAAGCAAGAAGTTCTTTATTGTCTTTTTGATCAAATTATACATTGTGCTGAAAATGTTGACATTGTTGAATTATTCAAGCATCTCCATTCAAAGGTAATGCTAGGTTTGCCCATGCTAGAAGGAACCAATCCTGCTTCATGCTTTCCATGTAGTGCTATCGGTTATGAAGCTGCCTGCTACAGTCGTGTATGGAGTGAGGTTTTTTCGGCCGATATTTTTGTCTCAAAGTTCCGCGGCGATCTTCTGAACCAGCATACTGGCTTGCAGTTCAGAAACAAGGTTTTGGCTCCGGGAGGTGCAAAAGAACCAATTGATATTCTCTCAGATTTTCTTGGGAGAGAACCATCAATTCAAGCTTTTATTGACACTAAGGCTGAATATAGCTTGTGA

Protein sequence

MTEIQGNNSEKMDKRKSQGKLLVFTGGAALLAVAVNLAIVAISRRKKKKDLPGFELRVNLSPSEILNLADRIIAKSKKVHDAVASVPPNKVTYSNVISPLADLEAEQFPLVQSCVFPKLISTSDDVRKASAEAERRIDAHVQMCSKRENVYRVVKAFSTRGEQTSAEQKCFIQCLVRDFERNGLNLTSTKREELLRLRVQIEELSLRYIQNLNDDGTFLPLSEAELDGLPKEFFESLDKTENGKFKVILRSHHIAAVLEHCKVGTTRRMVAMAYGKRCGEVNLSILENLVLLRHKFARLQGYSNYADYAVHYRMARSSSKVFEFLENISDSLTDLAAKELASLKDLKKQEEGESPFGIEDLLYYVKKVEDQELNLDFMTLKQYFPVSLVLSGIFKIMQDLFGLRFEEVIDAEVWHYDVKLYSVFDLNSGELIGYFFLDLYTREGKYIHTCVVALQNSALLYNGTRQIPVALLISQLEKDVGGHAGLMRFTEVVNLFHEFGHVVQHICNRASFTRISGLRVDPDFVEIPAQILENWCYESVSLKLLSGFHQDITVPIKDEVCESLKKWRHSFSALKLKQEVLYCLFDQIIHCAENVDIVELFKHLHSKVMLGLPMLEGTNPASCFPCSAIGYEAACYSRVWSEVFSADIFVSKFRGDLLNQHTGLQFRNKVLAPGGAKEPIDILSDFLGREPSIQAFIDTKAEYSL
Homology
BLAST of Lag0004695 vs. NCBI nr
Match: XP_022996581.1 (probable thimet oligopeptidase [Cucurbita maxima])

HSP 1 Score: 1323.5 bits (3424), Expect = 0.0e+00
Identity = 666/705 (94.47%), Postives = 686/705 (97.30%), Query Frame = 0

Query: 1   MTEIQGNNSEKMDKRKSQGKLLVFTGGAALLAVAVNLAIVAISRRKKKKDLPGFELRVNL 60
           MTEI+G NSEKMDKRKS+ +LLVFTGGAALLAVAVNLAIVAIS RKKKK+LPGFELRVNL
Sbjct: 1   MTEIEG-NSEKMDKRKSRSQLLVFTGGAALLAVAVNLAIVAISNRKKKKELPGFELRVNL 60

Query: 61  SPSEILNLADRIIAKSKKVHDAVASVPPNKVTYSNVISPLADLEAEQFPLVQSCVFPKLI 120
           S SEILNLADRIIAKSKKVHDAVASVPPNKVTYSNVISPLADLEAEQFP VQSCVFPKLI
Sbjct: 61  SASEILNLADRIIAKSKKVHDAVASVPPNKVTYSNVISPLADLEAEQFPQVQSCVFPKLI 120

Query: 121 STSDDVRKASAEAERRIDAHVQMCSKRENVYRVVKAFSTRGEQTSAEQKCFIQCLVRDFE 180
           STSDDVRKASAEAERRIDAHVQMCSKRE+VYRVVKAFSTRGEQTSAEQKCFIQCL+RDFE
Sbjct: 121 STSDDVRKASAEAERRIDAHVQMCSKREDVYRVVKAFSTRGEQTSAEQKCFIQCLIRDFE 180

Query: 181 RNGLNLTSTKREELLRLRVQIEELSLRYIQNLNDDGTFLPLSEAELDGLPKEFFESLDKT 240
           RNGLNLTS KREELLRLRVQIEELSLRYIQNLNDDGTF+PLSEAELDGLPKEFFESLDK 
Sbjct: 181 RNGLNLTSPKREELLRLRVQIEELSLRYIQNLNDDGTFIPLSEAELDGLPKEFFESLDKN 240

Query: 241 ENGKFKVILRSHHIAAVLEHCKVGTTRRMVAMAYGKRCGEVNLSILENLVLLRHKFARLQ 300
           ENG+FKV +RSHH AA+LEHCKVGTTRRMVAM YGKRCGEVNLSILENLV LRHKFARLQ
Sbjct: 241 ENGQFKVTMRSHHTAAILEHCKVGTTRRMVAMTYGKRCGEVNLSILENLVHLRHKFARLQ 300

Query: 301 GYSNYADYAVHYRMARSSSKVFEFLENISDSLTDLAAKELASLKDLKKQEEGESPFGIED 360
           GYSNYADYAVHYRMARSS+KVFEFLE+ISDSLTD+AAKELASLKDLKKQEEGESPFGIED
Sbjct: 301 GYSNYADYAVHYRMARSSAKVFEFLESISDSLTDVAAKELASLKDLKKQEEGESPFGIED 360

Query: 361 LLYYVKKVEDQELNLDFMTLKQYFPVSLVLSGIFKIMQDLFGLRFEEVIDAEVWHYDVKL 420
           LLYYVKKVEDQE NLDF+TLKQYFPVSLVLSGIFKIMQDLFGLRFEEVIDAEVWH DVKL
Sbjct: 361 LLYYVKKVEDQEFNLDFVTLKQYFPVSLVLSGIFKIMQDLFGLRFEEVIDAEVWHDDVKL 420

Query: 421 YSVFDLNSGELIGYFFLDLYTREGKYIHTCVVALQNSALLYNGTRQIPVALLISQLEKDV 480
           YSVFDLNSGELIGYFFLDLYTREGKYIHTCVVALQNSALL NGTRQIPVALLISQL+KDV
Sbjct: 421 YSVFDLNSGELIGYFFLDLYTREGKYIHTCVVALQNSALLSNGTRQIPVALLISQLQKDV 480

Query: 481 GGHAGLMRFTEVVNLFHEFGHVVQHICNRASFTRISGLRVDPDFVEIPAQILENWCYESV 540
            GHAGLMRFTEVVNLFHEFGHVVQHICN ASFTRISGLRVD DFVEIPAQILENWCYESV
Sbjct: 481 DGHAGLMRFTEVVNLFHEFGHVVQHICNHASFTRISGLRVDSDFVEIPAQILENWCYESV 540

Query: 541 SLKLLSGFHQDITVPIKDEVCESLKKWRHSFSALKLKQEVLYCLFDQIIHCAENVDIVEL 600
           SLKLLSGFHQDITVPIKDEVCESLKKWRHSFSALKLKQEVLYCLFDQIIHCAENVDI+EL
Sbjct: 541 SLKLLSGFHQDITVPIKDEVCESLKKWRHSFSALKLKQEVLYCLFDQIIHCAENVDIIEL 600

Query: 601 FKHLHSKVMLGLPMLEGTNPASCFPCSAIGYEAACYSRVWSEVFSADIFVSKFRGDLLNQ 660
           FKHLHSKVMLGLPMLEG+NPASCFPCSAIGYEAACYSRVWSEVF+ADIFVSKFRGDLLNQ
Sbjct: 601 FKHLHSKVMLGLPMLEGSNPASCFPCSAIGYEAACYSRVWSEVFAADIFVSKFRGDLLNQ 660

Query: 661 HTGLQFRNKVLAPGGAKEPIDILSDFLGREPSIQAFIDTKAEYSL 706
           H GLQFRNKVL PGGAKEPID+LSDFLGREPSIQAFID+KAEYSL
Sbjct: 661 HIGLQFRNKVLVPGGAKEPIDVLSDFLGREPSIQAFIDSKAEYSL 704

BLAST of Lag0004695 vs. NCBI nr
Match: KAG6598263.1 (putative thimet oligopeptidase, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 1322.4 bits (3421), Expect = 0.0e+00
Identity = 666/705 (94.47%), Postives = 687/705 (97.45%), Query Frame = 0

Query: 1   MTEIQGNNSEKMDKRKSQGKLLVFTGGAALLAVAVNLAIVAISRRKKKKDLPGFELRVNL 60
           MTEI+G NSEKMDKRKS+ +LLVFTGGAALLAVAVNLAIVAIS RKKKK+LPGFELRVNL
Sbjct: 1   MTEIEG-NSEKMDKRKSRSQLLVFTGGAALLAVAVNLAIVAISNRKKKKELPGFELRVNL 60

Query: 61  SPSEILNLADRIIAKSKKVHDAVASVPPNKVTYSNVISPLADLEAEQFPLVQSCVFPKLI 120
           S SEILNLADRIIAKSKKVHDAVASVPPNKVTYSNVISPLADLEAEQFP VQSCVFPKLI
Sbjct: 61  SASEILNLADRIIAKSKKVHDAVASVPPNKVTYSNVISPLADLEAEQFPQVQSCVFPKLI 120

Query: 121 STSDDVRKASAEAERRIDAHVQMCSKRENVYRVVKAFSTRGEQTSAEQKCFIQCLVRDFE 180
           STSDDVRKASAEAE RIDAHVQMCSKRE+VYRVVKAFSTRGEQTSAEQKCFIQCLVRDFE
Sbjct: 121 STSDDVRKASAEAEWRIDAHVQMCSKREDVYRVVKAFSTRGEQTSAEQKCFIQCLVRDFE 180

Query: 181 RNGLNLTSTKREELLRLRVQIEELSLRYIQNLNDDGTFLPLSEAELDGLPKEFFESLDKT 240
           RNGLNLTS+KREELLRLRVQIEELSLRYIQNLNDDGTF+PLSEAELDGLPKEFFESLDK 
Sbjct: 181 RNGLNLTSSKREELLRLRVQIEELSLRYIQNLNDDGTFIPLSEAELDGLPKEFFESLDKN 240

Query: 241 ENGKFKVILRSHHIAAVLEHCKVGTTRRMVAMAYGKRCGEVNLSILENLVLLRHKFARLQ 300
           ENG+FKV +RSHH AA+LEHCKVG TRRMVAM YGKRCGEVNLSILENLV LRHKFARLQ
Sbjct: 241 ENGQFKVTMRSHHTAAILEHCKVGMTRRMVAMTYGKRCGEVNLSILENLVHLRHKFARLQ 300

Query: 301 GYSNYADYAVHYRMARSSSKVFEFLENISDSLTDLAAKELASLKDLKKQEEGESPFGIED 360
           GYSNYADYAVHYRMARSS+KVFEFLE+ISDSLTD+AAKELASLKDLKKQEEGESPFGIED
Sbjct: 301 GYSNYADYAVHYRMARSSAKVFEFLESISDSLTDVAAKELASLKDLKKQEEGESPFGIED 360

Query: 361 LLYYVKKVEDQELNLDFMTLKQYFPVSLVLSGIFKIMQDLFGLRFEEVIDAEVWHYDVKL 420
           LLYYVKKVEDQE NLDF+TLKQYFPVSLVLSGIFKIMQDLFGLRFEEVIDAEVWH DVKL
Sbjct: 361 LLYYVKKVEDQEFNLDFVTLKQYFPVSLVLSGIFKIMQDLFGLRFEEVIDAEVWHGDVKL 420

Query: 421 YSVFDLNSGELIGYFFLDLYTREGKYIHTCVVALQNSALLYNGTRQIPVALLISQLEKDV 480
           YSVFDLNSGELIGYFFLDLYTREGKYIHTCVVALQNSALL NGTRQIPVALLISQL+KDV
Sbjct: 421 YSVFDLNSGELIGYFFLDLYTREGKYIHTCVVALQNSALLSNGTRQIPVALLISQLQKDV 480

Query: 481 GGHAGLMRFTEVVNLFHEFGHVVQHICNRASFTRISGLRVDPDFVEIPAQILENWCYESV 540
            GHAGLMRFTEVVNLFHEFGHVVQHICN ASFTRISGLRVDPDFVEIPAQILENWCYESV
Sbjct: 481 DGHAGLMRFTEVVNLFHEFGHVVQHICNHASFTRISGLRVDPDFVEIPAQILENWCYESV 540

Query: 541 SLKLLSGFHQDITVPIKDEVCESLKKWRHSFSALKLKQEVLYCLFDQIIHCAENVDIVEL 600
           SLKLLSGFHQDITVPIKDEVCESLKKWRHSFSALKLKQEVLYCLFDQIIHCAENVDI+EL
Sbjct: 541 SLKLLSGFHQDITVPIKDEVCESLKKWRHSFSALKLKQEVLYCLFDQIIHCAENVDIIEL 600

Query: 601 FKHLHSKVMLGLPMLEGTNPASCFPCSAIGYEAACYSRVWSEVFSADIFVSKFRGDLLNQ 660
           FKHLHSKVMLGLPMLEG+NPASCFPCSAIG+EAACYSRVWSEVF+ADIFVSKFRGDLLNQ
Sbjct: 601 FKHLHSKVMLGLPMLEGSNPASCFPCSAIGFEAACYSRVWSEVFAADIFVSKFRGDLLNQ 660

Query: 661 HTGLQFRNKVLAPGGAKEPIDILSDFLGREPSIQAFIDTKAEYSL 706
           H GLQFRNKVLAPGGAKEPID+LSDFLGREPSIQAFID+KAEYSL
Sbjct: 661 HIGLQFRNKVLAPGGAKEPIDVLSDFLGREPSIQAFIDSKAEYSL 704

BLAST of Lag0004695 vs. NCBI nr
Match: XP_022962337.1 (probable thimet oligopeptidase [Cucurbita moschata])

HSP 1 Score: 1315.8 bits (3404), Expect = 0.0e+00
Identity = 663/705 (94.04%), Postives = 685/705 (97.16%), Query Frame = 0

Query: 1   MTEIQGNNSEKMDKRKSQGKLLVFTGGAALLAVAVNLAIVAISRRKKKKDLPGFELRVNL 60
           M EI+G NSEKMDKRKS+ +LLVFTGGAALLAVAVNLAIVAIS RKKKK+LPGFELRVNL
Sbjct: 1   MKEIEG-NSEKMDKRKSRSQLLVFTGGAALLAVAVNLAIVAISNRKKKKELPGFELRVNL 60

Query: 61  SPSEILNLADRIIAKSKKVHDAVASVPPNKVTYSNVISPLADLEAEQFPLVQSCVFPKLI 120
           S SEILNLADRIIAKSKKVHDAVASVPPNKVTYSNVISPLADLEAEQFP VQSCVFPKLI
Sbjct: 61  SASEILNLADRIIAKSKKVHDAVASVPPNKVTYSNVISPLADLEAEQFPQVQSCVFPKLI 120

Query: 121 STSDDVRKASAEAERRIDAHVQMCSKRENVYRVVKAFSTRGEQTSAEQKCFIQCLVRDFE 180
           STSDDVRKASAEAE RIDAHVQMCSKRE+VYRVVKAFSTRGEQTSAEQKCFIQCLVRDFE
Sbjct: 121 STSDDVRKASAEAEWRIDAHVQMCSKREDVYRVVKAFSTRGEQTSAEQKCFIQCLVRDFE 180

Query: 181 RNGLNLTSTKREELLRLRVQIEELSLRYIQNLNDDGTFLPLSEAELDGLPKEFFESLDKT 240
           RNGLNLTS+KR+ELLRL VQIEELSLRYIQNLNDDGTF+PLSEAELDGLPKEFFESLDK 
Sbjct: 181 RNGLNLTSSKRKELLRLGVQIEELSLRYIQNLNDDGTFIPLSEAELDGLPKEFFESLDKN 240

Query: 241 ENGKFKVILRSHHIAAVLEHCKVGTTRRMVAMAYGKRCGEVNLSILENLVLLRHKFARLQ 300
           ENG+FKV +RSHH AA+LEHCKVG TRRMVAM YGKRCGEVNLSILENLV LRHKFARLQ
Sbjct: 241 ENGQFKVTMRSHHTAAILEHCKVGMTRRMVAMTYGKRCGEVNLSILENLVHLRHKFARLQ 300

Query: 301 GYSNYADYAVHYRMARSSSKVFEFLENISDSLTDLAAKELASLKDLKKQEEGESPFGIED 360
           GYSNYADYAVHYRMARSS+KVFEFLE+ISDSLTD+AAKELASLKDLKKQEEGESPFGIED
Sbjct: 301 GYSNYADYAVHYRMARSSAKVFEFLESISDSLTDVAAKELASLKDLKKQEEGESPFGIED 360

Query: 361 LLYYVKKVEDQELNLDFMTLKQYFPVSLVLSGIFKIMQDLFGLRFEEVIDAEVWHYDVKL 420
           LLYYVKKVEDQE NLDF+TLKQYFPVSLVLSGIFKIMQDLFGLRFEEVIDAEVWH DVKL
Sbjct: 361 LLYYVKKVEDQEFNLDFVTLKQYFPVSLVLSGIFKIMQDLFGLRFEEVIDAEVWHGDVKL 420

Query: 421 YSVFDLNSGELIGYFFLDLYTREGKYIHTCVVALQNSALLYNGTRQIPVALLISQLEKDV 480
           YSVFDLNSGELIGYFFLDLYTREGKYIHTCVVALQNSALL NGTRQIPVALLISQL+KDV
Sbjct: 421 YSVFDLNSGELIGYFFLDLYTREGKYIHTCVVALQNSALLSNGTRQIPVALLISQLQKDV 480

Query: 481 GGHAGLMRFTEVVNLFHEFGHVVQHICNRASFTRISGLRVDPDFVEIPAQILENWCYESV 540
            GHAGLMRFTEVVNLFHEFGHVVQHICN ASFTRISGLRVDPDFVEIPAQILENWCYESV
Sbjct: 481 DGHAGLMRFTEVVNLFHEFGHVVQHICNHASFTRISGLRVDPDFVEIPAQILENWCYESV 540

Query: 541 SLKLLSGFHQDITVPIKDEVCESLKKWRHSFSALKLKQEVLYCLFDQIIHCAENVDIVEL 600
           SLKLLSGFHQDITVPIKDEVCESLKKWRHSFSALKLKQEVLYCLFDQIIHCAENVDI+EL
Sbjct: 541 SLKLLSGFHQDITVPIKDEVCESLKKWRHSFSALKLKQEVLYCLFDQIIHCAENVDIIEL 600

Query: 601 FKHLHSKVMLGLPMLEGTNPASCFPCSAIGYEAACYSRVWSEVFSADIFVSKFRGDLLNQ 660
           FKHLHSKVMLGLPMLEG+NPASCFPCSAIG+EAACYSRVWSEVF+ADIFVSKFRGDLLNQ
Sbjct: 601 FKHLHSKVMLGLPMLEGSNPASCFPCSAIGFEAACYSRVWSEVFAADIFVSKFRGDLLNQ 660

Query: 661 HTGLQFRNKVLAPGGAKEPIDILSDFLGREPSIQAFIDTKAEYSL 706
           H GLQFRNKVLAPGGAKEPID+LSDFLGREPSIQAFID+KAEYSL
Sbjct: 661 HIGLQFRNKVLAPGGAKEPIDVLSDFLGREPSIQAFIDSKAEYSL 704

BLAST of Lag0004695 vs. NCBI nr
Match: XP_023546722.1 (probable thimet oligopeptidase [Cucurbita pepo subsp. pepo])

HSP 1 Score: 1315.1 bits (3402), Expect = 0.0e+00
Identity = 662/705 (93.90%), Postives = 684/705 (97.02%), Query Frame = 0

Query: 1   MTEIQGNNSEKMDKRKSQGKLLVFTGGAALLAVAVNLAIVAISRRKKKKDLPGFELRVNL 60
           MTEI+G NSEKMDKRKS+ +LLVFTGGAALLAVAVNL IVAIS RKKKK+ PGFELRVNL
Sbjct: 1   MTEIEG-NSEKMDKRKSRSQLLVFTGGAALLAVAVNLVIVAISNRKKKKEFPGFELRVNL 60

Query: 61  SPSEILNLADRIIAKSKKVHDAVASVPPNKVTYSNVISPLADLEAEQFPLVQSCVFPKLI 120
           S SEILNLADRIIAKSKKVHDAVASVPPNKVTYSNVISPLADLEAEQFP VQSCVFPKLI
Sbjct: 61  SASEILNLADRIIAKSKKVHDAVASVPPNKVTYSNVISPLADLEAEQFPQVQSCVFPKLI 120

Query: 121 STSDDVRKASAEAERRIDAHVQMCSKRENVYRVVKAFSTRGEQTSAEQKCFIQCLVRDFE 180
           STSDDVRKASAEAERRIDAHVQMCSKRE+VYRVVKAFST GEQTSAEQKCFIQCLVRDFE
Sbjct: 121 STSDDVRKASAEAERRIDAHVQMCSKREDVYRVVKAFSTSGEQTSAEQKCFIQCLVRDFE 180

Query: 181 RNGLNLTSTKREELLRLRVQIEELSLRYIQNLNDDGTFLPLSEAELDGLPKEFFESLDKT 240
           RNGLNLTS+KREELLRLRVQIEELSLRYIQNLNDDGTF+PLSEAELDGLPKEFFESLDK 
Sbjct: 181 RNGLNLTSSKREELLRLRVQIEELSLRYIQNLNDDGTFIPLSEAELDGLPKEFFESLDKN 240

Query: 241 ENGKFKVILRSHHIAAVLEHCKVGTTRRMVAMAYGKRCGEVNLSILENLVLLRHKFARLQ 300
           ENG+FKV +RSHH AA+LEHCKVGTTRRMVAM YGKRCGEVNLSILENLV LRHKFARLQ
Sbjct: 241 ENGQFKVTMRSHHTAAILEHCKVGTTRRMVAMTYGKRCGEVNLSILENLVHLRHKFARLQ 300

Query: 301 GYSNYADYAVHYRMARSSSKVFEFLENISDSLTDLAAKELASLKDLKKQEEGESPFGIED 360
           GYSNYADYAVHYRMARSS+KVFEFLE+ISDSLTD+AAKELASLKDLKKQEEGESPFGIED
Sbjct: 301 GYSNYADYAVHYRMARSSAKVFEFLESISDSLTDVAAKELASLKDLKKQEEGESPFGIED 360

Query: 361 LLYYVKKVEDQELNLDFMTLKQYFPVSLVLSGIFKIMQDLFGLRFEEVIDAEVWHYDVKL 420
           LLYYVKKVEDQE NLDF+TLKQYFPVSLVLSGIFKIMQDLFGL FEEVIDAEVWH DVKL
Sbjct: 361 LLYYVKKVEDQEFNLDFVTLKQYFPVSLVLSGIFKIMQDLFGLWFEEVIDAEVWHGDVKL 420

Query: 421 YSVFDLNSGELIGYFFLDLYTREGKYIHTCVVALQNSALLYNGTRQIPVALLISQLEKDV 480
           Y VFDLNSGELIGYFFLDLYTREGKYIHTCVVALQNSALL NGTRQIPVALLISQL+KDV
Sbjct: 421 YLVFDLNSGELIGYFFLDLYTREGKYIHTCVVALQNSALLSNGTRQIPVALLISQLQKDV 480

Query: 481 GGHAGLMRFTEVVNLFHEFGHVVQHICNRASFTRISGLRVDPDFVEIPAQILENWCYESV 540
            GHAGLMRFTEVVNLFHEFGHVVQHICN ASFTRISGLRVDPDFVEIPAQILENWCYESV
Sbjct: 481 DGHAGLMRFTEVVNLFHEFGHVVQHICNHASFTRISGLRVDPDFVEIPAQILENWCYESV 540

Query: 541 SLKLLSGFHQDITVPIKDEVCESLKKWRHSFSALKLKQEVLYCLFDQIIHCAENVDIVEL 600
           SLKLLSGFHQDITVPIKDEVCESLKKWRHSFSALKLKQEVLYCLFDQIIHCAENVDI+EL
Sbjct: 541 SLKLLSGFHQDITVPIKDEVCESLKKWRHSFSALKLKQEVLYCLFDQIIHCAENVDIIEL 600

Query: 601 FKHLHSKVMLGLPMLEGTNPASCFPCSAIGYEAACYSRVWSEVFSADIFVSKFRGDLLNQ 660
           FKHLHSKVMLGLPMLEG+NPASCFPCSAIG+EAACYSRVWSEVF+ADIFVSKFRGDLLNQ
Sbjct: 601 FKHLHSKVMLGLPMLEGSNPASCFPCSAIGFEAACYSRVWSEVFAADIFVSKFRGDLLNQ 660

Query: 661 HTGLQFRNKVLAPGGAKEPIDILSDFLGREPSIQAFIDTKAEYSL 706
           H GLQFRNKVLAPGGAKEPID+LSDFLGREPSIQAFID+KAE+SL
Sbjct: 661 HIGLQFRNKVLAPGGAKEPIDVLSDFLGREPSIQAFIDSKAEHSL 704

BLAST of Lag0004695 vs. NCBI nr
Match: XP_004142949.1 (probable thimet oligopeptidase isoform X1 [Cucumis sativus] >KGN62354.1 hypothetical protein Csa_018706 [Cucumis sativus])

HSP 1 Score: 1311.2 bits (3392), Expect = 0.0e+00
Identity = 655/705 (92.91%), Postives = 684/705 (97.02%), Query Frame = 0

Query: 1   MTEIQGNNSEKMDKRKSQGKLLVFTGGAALLAVAVNLAIVAISRRKKKKDLPGFELRVNL 60
           MTEIQG N++KM KRK+Q KLLVFTGGAALLAVAVNLAIVAI +RKKKK+LPGFELR NL
Sbjct: 1   MTEIQG-NTQKMYKRKTQSKLLVFTGGAALLAVAVNLAIVAICKRKKKKELPGFELRPNL 60

Query: 61  SPSEILNLADRIIAKSKKVHDAVASVPPNKVTYSNVISPLADLEAEQFPLVQSCVFPKLI 120
           S SEILNLAD+IIAKSKKVHDAVASVPPNKVTYSNVISPLADLEAEQFPLVQSCVFPKLI
Sbjct: 61  SASEILNLADKIIAKSKKVHDAVASVPPNKVTYSNVISPLADLEAEQFPLVQSCVFPKLI 120

Query: 121 STSDDVRKASAEAERRIDAHVQMCSKRENVYRVVKAFSTRGEQTSAEQKCFIQCLVRDFE 180
           STSDDVR ASAEAERRIDAH QMCSKRE+VYRVVKAFS RGEQTSAEQKCFIQCLVRDFE
Sbjct: 121 STSDDVRAASAEAERRIDAHAQMCSKREDVYRVVKAFSARGEQTSAEQKCFIQCLVRDFE 180

Query: 181 RNGLNLTSTKREELLRLRVQIEELSLRYIQNLNDDGTFLPLSEAELDGLPKEFFESLDKT 240
           RNGLNLT++KR+ELLRLRVQIEELSLRYIQNLNDDGTF+P SEAELDGLPKEFFESLDKT
Sbjct: 181 RNGLNLTTSKRKELLRLRVQIEELSLRYIQNLNDDGTFIPFSEAELDGLPKEFFESLDKT 240

Query: 241 ENGKFKVILRSHHIAAVLEHCKVGTTRRMVAMAYGKRCGEVNLSILENLVLLRHKFARLQ 300
           ENGKFKV++RSHH A VLEHCKVGTTRRMVAMAYGKRCGEVNLSILENLV LRHKFARLQ
Sbjct: 241 ENGKFKVVMRSHHTAVVLEHCKVGTTRRMVAMAYGKRCGEVNLSILENLVHLRHKFARLQ 300

Query: 301 GYSNYADYAVHYRMARSSSKVFEFLENISDSLTDLAAKELASLKDLKKQEEGESPFGIED 360
           GYSNYADYAVHYRMARSS+KVFEFLENISDS+TDLAAKELASLK+LKKQEEGESPFGIED
Sbjct: 301 GYSNYADYAVHYRMARSSAKVFEFLENISDSITDLAAKELASLKNLKKQEEGESPFGIED 360

Query: 361 LLYYVKKVEDQELNLDFMTLKQYFPVSLVLSGIFKIMQDLFGLRFEEVIDAEVWHYDVKL 420
           LLYYVK+ EDQE NLDF+T+KQYFPVSLVLSGIFKIMQDLFGLRFEEVIDAEVWHYDVKL
Sbjct: 361 LLYYVKRAEDQEFNLDFVTVKQYFPVSLVLSGIFKIMQDLFGLRFEEVIDAEVWHYDVKL 420

Query: 421 YSVFDLNSGELIGYFFLDLYTREGKYIHTCVVALQNSALLYNGTRQIPVALLISQLEKDV 480
           YSVFDLNSGELIGYFFLDLYTRE KYIHTCVVALQ+SALL NGTRQIPVALL+SQL+ DV
Sbjct: 421 YSVFDLNSGELIGYFFLDLYTREEKYIHTCVVALQSSALLSNGTRQIPVALLLSQLQNDV 480

Query: 481 GGHAGLMRFTEVVNLFHEFGHVVQHICNRASFTRISGLRVDPDFVEIPAQILENWCYESV 540
            GHAGLMRFTEVVNLFHEFGHVVQH+CNRA FTRISGLR+DPDFVEIPAQILENWCYESV
Sbjct: 481 DGHAGLMRFTEVVNLFHEFGHVVQHVCNRAPFTRISGLRLDPDFVEIPAQILENWCYESV 540

Query: 541 SLKLLSGFHQDITVPIKDEVCESLKKWRHSFSALKLKQEVLYCLFDQIIHCAENVDIVEL 600
           SLKLLSGFHQDITVPIKDEVCESLKKWRHSFSALKLKQE+LYCLFDQIIHCAENVDI+EL
Sbjct: 541 SLKLLSGFHQDITVPIKDEVCESLKKWRHSFSALKLKQEILYCLFDQIIHCAENVDIIEL 600

Query: 601 FKHLHSKVMLGLPMLEGTNPASCFPCSAIGYEAACYSRVWSEVFSADIFVSKFRGDLLNQ 660
           FKHLHSKVMLGLPMLEGTNPASCFPCSAIGYEAACYSRVWSEVFSADIFVSKFRG+LLNQ
Sbjct: 601 FKHLHSKVMLGLPMLEGTNPASCFPCSAIGYEAACYSRVWSEVFSADIFVSKFRGNLLNQ 660

Query: 661 HTGLQFRNKVLAPGGAKEPIDILSDFLGREPSIQAFIDTKAEYSL 706
           H GLQFRNKVLAPGGAKEPID+LSDFLGREPSIQAFID+KAEYSL
Sbjct: 661 HIGLQFRNKVLAPGGAKEPIDVLSDFLGREPSIQAFIDSKAEYSL 704

BLAST of Lag0004695 vs. ExPASy Swiss-Prot
Match: F4HTQ1 (Probable thimet oligopeptidase OS=Arabidopsis thaliana OX=3702 GN=At1g67690 PE=3 SV=1)

HSP 1 Score: 875.9 bits (2262), Expect = 3.0e-253
Identity = 426/710 (60.00%), Postives = 562/710 (79.15%), Query Frame = 0

Query: 1   MTEIQGNNSE-KMDKRKSQGKLLVFTGGAALLAVAVNLAIVAISRRK---KKKDLPGFE- 60
           MTE +GN+ + +    K +  ++ FTG A LL +AV+ AI      K   KKK LPG + 
Sbjct: 1   MTENEGNDKKIEGSNPKKKLNVVTFTGAAGLLGLAVSFAIFTFKSHKQKSKKKGLPGCDT 60

Query: 61  LRVNLSPSEILNLADRIIAKSKKVHDAVASVPPNKVTYSNVISPLADLEAEQFPLVQSCV 120
           + VNLS  EIL+LA+ II KS +VHDAVA V  +K++Y NV+ PLA+LEA Q  L+Q CV
Sbjct: 61  VCVNLSAKEILDLAEEIIHKSTRVHDAVALVSLDKLSYENVVLPLAELEARQLSLIQCCV 120

Query: 121 FPKLISTSDDVRKASAEAERRIDAHVQMCSKRENVYRVVKAFSTRGEQTSAEQKCFIQCL 180
           FPK++S  D+VRKAS EAE++IDAH+  C KRE+VYR++K ++ +GE  S E KC++QCL
Sbjct: 121 FPKMLSPHDNVRKASTEAEQKIDAHILSCRKREDVYRIIKIYAAKGESISPEAKCYLQCL 180

Query: 181 VRDFERNGLNLTSTKREELLRLRVQIEELSLRYIQNLNDDGTFLPLSEAELDGLPKEFFE 240
           VRDFE NGLNLT+ KREE+ RL+ +I+ELSLRYIQNLN+D + L  +E EL GLP EF +
Sbjct: 181 VRDFEDNGLNLTAIKREEVERLKYEIDELSLRYIQNLNEDSSCLFFTEDELAGLPLEFLQ 240

Query: 241 SLDKTENGKFKVILRSHHIAAVLEHCKVGTTRRMVAMAYGKRCGEVNLSILENLVLLRHK 300
           +L+KT+N +FK+ L S H+AA+LE CK+  TR+ VAMAYGKRCG+ N+ +L+ LV  RH+
Sbjct: 241 NLEKTQNKEFKLTLESRHVAAILELCKIAKTRKTVAMAYGKRCGDTNIPVLQRLVQSRHR 300

Query: 301 FARLQGYSNYADYAVHYRMARSSSKVFEFLENISDSLTDLAAKELASLKDLKKQEEGESP 360
            A + GY+++ADYA+  RM+++S +V  FLE+IS SLTDLA +E + L+DLK++EEGE P
Sbjct: 301 LACVCGYAHFADYALDRRMSKTSMRVIRFLEDISSSLTDLAIREFSILEDLKRKEEGEIP 360

Query: 361 FGIEDLLYYVKKVEDQELNLDFMTLKQYFPVSLVLSGIFKIMQDLFGLRFEEVIDAEVWH 420
           FG+EDLLYY+K+VE+ + +LDF  ++QYFPV+LVLSGIFKI QDLFG++FEEV + +VW+
Sbjct: 361 FGVEDLLYYIKRVEELQFDLDFGDIRQYFPVNLVLSGIFKICQDLFGIKFEEVTEVDVWY 420

Query: 421 YDVKLYSVFDLNSGELIGYFFLDLYTREGKYIHTCVVALQNSALLYNGTRQIPVALLISQ 480
           +D++ ++VFD  SG+L+GYF+LD++TREGK  H+CVVALQN+AL  NG  QIPVALLI+Q
Sbjct: 421 HDIRAFAVFDSGSGKLLGYFYLDMFTREGKCNHSCVVALQNNALFSNGACQIPVALLIAQ 480

Query: 481 LEKDVGGHAGLMRFTEVVNLFHEFGHVVQHICNRASFTRISGLRVDPDFVEIPAQILENW 540
             KD  G A  + F++VVNLFHEFGHVVQHICNRASF R SGLRVDPDF EIP+Q+LENW
Sbjct: 481 FAKDGSGEAVPLGFSDVVNLFHEFGHVVQHICNRASFARFSGLRVDPDFREIPSQLLENW 540

Query: 541 CYESVSLKLLSGFHQDITVPIKDEVCESLKKWRHSFSALKLKQEVLYCLFDQIIHCAENV 600
           CYES +LKL+SG+ QDIT P+ DEVC++LK+WR+SFSALK  QE+LYCLFDQII+  ++ 
Sbjct: 541 CYESFTLKLISGYRQDITKPLVDEVCKTLKRWRYSFSALKSLQEILYCLFDQIIYSDDDA 600

Query: 601 DIVELFKHLHSKVMLGLPMLEGTNPASCFPCSAIGYEAACYSRVWSEVFSADIFVSKFRG 660
           D+++L + LH KVM+GLP++EGTNPASCFP + IG EA CYSR+WSEV++ADIF SKF  
Sbjct: 601 DLLQLIRSLHPKVMIGLPVVEGTNPASCFPRAVIGSEATCYSRLWSEVYAADIFASKFGD 660

Query: 661 DLLNQHTGLQFRNKVLAPGGAKEPIDILSDFLGREPSIQAFIDTKAEYSL 706
              N + GLQFR+KVLAPGG KEP+++L++FLGREPS QAFI ++  YSL
Sbjct: 661 GHPNLYAGLQFRDKVLAPGGGKEPMELLTNFLGREPSTQAFIASRTNYSL 710

BLAST of Lag0004695 vs. ExPASy Swiss-Prot
Match: P42675 (Neurolysin, mitochondrial OS=Oryctolagus cuniculus OX=9986 GN=NLN PE=1 SV=1)

HSP 1 Score: 434.1 bits (1115), Expect = 3.0e-120
Identity = 243/651 (37.33%), Postives = 383/651 (58.83%), Query Frame = 0

Query: 56  LRVNLSPSEILNLADRIIAKSKKVHDAVASVPPNKVTYSNVISPLADLEAEQFPLVQSCV 115
           LR +LSP +I    + +IA++K+V+D+V  +    VTY N +  LAD+E +         
Sbjct: 48  LRWDLSPEQIKTRTEELIAQTKQVYDSVGMLDIKDVTYENCLQALADVEVKYIVERTMLD 107

Query: 116 FPKLISTSDDVRKASAEAERRIDAHVQMCSKRENVY-RVVKAFSTRG-EQTSAEQKCFIQ 175
           FP+ +ST  +VR AS EA++R+       S RE+++ R+V    T   E+   E + +++
Sbjct: 108 FPQHVSTDREVRAASTEADKRLSRFDIEMSMREDIFQRIVHLQETCDLEKIKPEARRYLE 167

Query: 176 CLVRDFERNGLNLTSTKREELLRLRVQIEELSLRYIQNLNDDGTFLPLSEAELDGLPKEF 235
             V+   RNGL+L    + E+  ++ ++ EL + + +NLN+D TFL  S+AEL  LP +F
Sbjct: 168 KSVKMGRRNGLHLPEEVQNEIKSMKKRMSELCIDFNKNLNEDDTFLVFSKAELGALPDDF 227

Query: 236 FESLDKTENGKFKVILRSHHIAAVLEHCKVGTTRRMVAMAYGKRCGEVNLSILENLVLLR 295
            +SL+K ++ K+K+ L+  H   V++ C +  TRR + MA+  RC E N  IL+ L+ LR
Sbjct: 228 IDSLEKMDDDKYKITLKYPHYFPVMKKCCIPETRRRMEMAFNTRCKEENTVILQQLLPLR 287

Query: 296 HKFARLQGYSNYADYAVHYRMARSSSKVFEFLENISDSLTDLAAKELASLKDLKKQEEGE 355
            + A+L GYS +AD+ +    A+S+S+V  FL+++S  L  L   E   +  LKK+E  E
Sbjct: 288 AQVAKLLGYSTHADFVLEMNTAKSTSRVTAFLDDLSQKLKPLGEAEREFILSLKKKECEE 347

Query: 356 SPFGIE------DLLYYVKKVEDQELNLDFMTLKQYFPVSLVLSGIFKIMQDLFGLRFEE 415
             F  +      DL YY+ + E+ + ++D   +K+YFP+ +V  G+  I Q+L GL FE+
Sbjct: 348 KGFEYDGKINAWDLHYYMTQTEELKYSIDQEFIKEYFPIEVVTEGLLNIYQELLGLSFEQ 407

Query: 416 VIDAEVWHYDVKLYSVFDLNSGELIGYFFLDLYTREGKYIHTCVVALQNSALLYNGTRQI 475
           V DA VW+  V LY+V D  +GE++G F+LDLY REGKY H     LQ   LL +G+R +
Sbjct: 408 VADAHVWNPSVTLYTVKDKATGEVLGQFYLDLYPREGKYNHAACFGLQPGCLLPDGSRML 467

Query: 476 PVALLISQLEKDVGGHAGLMRFTEVVNLFHEFGHVVQHICNRASFTRISGLRVDPDFVEI 535
            VA L+    + V G   L+R  EV   FHEFGHV+  IC +  F R SG  V+ DFVE+
Sbjct: 468 SVAALVVNFSQPVAGRPSLLRHDEVRTYFHEFGHVMHQICAQTDFARFSGTNVETDFVEV 527

Query: 536 PAQILENWCYESVSLKLLSGFHQDITVPIKDEVCESLKKWRHSFSALKLKQEVLYCLFDQ 595
           P+Q+LENW ++  SL+ LS  ++D   PI D++ E L   R   + L   ++++    DQ
Sbjct: 528 PSQMLENWVWDIDSLRRLSKHYKDGN-PIADDLLEKLVASRLVNTGLLTLRQIVLSKVDQ 587

Query: 596 IIHCAENVDIVELFKHLHSKVMLGLPMLEGTNPASCFPCSAIGYEAACYSRVWSEVFSAD 655
            +H   ++D    +    + + LG+    GTN  + F   A GY+   Y  +WSEVFS D
Sbjct: 588 SLHTNSSLDAASEYARYCTDI-LGVAATPGTNMPATFGHLAGGYDGQYYGYLWSEVFSMD 647

Query: 656 IFVSKFRGD-LLNQHTGLQFRNKVLAPGGAKEPIDILSDFLGREPSIQAFI 698
           +F S F+ + ++N   G+++RN +L PGG+ + +D+L +FL REP+ +AF+
Sbjct: 648 MFYSCFKKEGIMNPEVGMKYRNLILRPGGSLDGMDMLQNFLQREPNQKAFL 696

BLAST of Lag0004695 vs. ExPASy Swiss-Prot
Match: Q91YP2 (Neurolysin, mitochondrial OS=Mus musculus OX=10090 GN=Nln PE=1 SV=1)

HSP 1 Score: 433.3 bits (1113), Expect = 5.1e-120
Identity = 245/651 (37.63%), Postives = 384/651 (58.99%), Query Frame = 0

Query: 56  LRVNLSPSEILNLADRIIAKSKKVHDAVASVPPNKVTYSNVISPLADLEAEQFPLVQSCV 115
           LR +LSP +I    + +IA++K+V+D V ++    VTY N +  LAD+E +         
Sbjct: 48  LRWDLSPEQIRTRTEELIAQTKQVYDTVGTINLEDVTYENCLQVLADIEVKYIVERTMLD 107

Query: 116 FPKLISTSDDVRKASAEAERRIDAHVQMCSKRENVY-RVVKAFSTRG-EQTSAEQKCFIQ 175
           FP+ +S+  +VR AS EA++R+       S RE+V+ R+V    T   E+   E + +++
Sbjct: 108 FPQHVSSDREVRAASTEADKRLSRFDIEMSMREDVFQRIVHLQETCDLEKIKPEARRYLE 167

Query: 176 CLVRDFERNGLNLTSTKREELLRLRVQIEELSLRYIQNLNDDGTFLPLSEAELDGLPKEF 235
             ++  +RNGL+L    + E+  ++ ++ EL + + +NLN+D T L  S+AEL  LP +F
Sbjct: 168 KSIKMGKRNGLHLPEHVKNEIKSMKKRMSELCIDFNKNLNEDDTSLVFSKAELGALPDDF 227

Query: 236 FESLDKTENGKFKVILRSHHIAAVLEHCKVGTTRRMVAMAYGKRCGEVNLSILENLVLLR 295
            +SL+KT+  K+KV L+  H   V++ C V  TRR + MA+  RC E N  IL+ L+ LR
Sbjct: 228 IDSLEKTDEDKYKVTLKYPHYFPVMKKCCVPETRRKMEMAFHTRCKEENTIILQQLLPLR 287

Query: 296 HKFARLQGYSNYADYAVHYRMARSSSKVFEFLENISDSLTDLAAKELASLKDLKKQEEGE 355
            + A+L GY+ +AD+ +    A+S+S V  FL+++S  L  L   E   +  LKK+E  E
Sbjct: 288 AQVAKLLGYNTHADFVLELNTAKSTSHVATFLDDLSQKLKPLGEAEREFILSLKKKECEE 347

Query: 356 SPFGIE------DLLYYVKKVEDQELNLDFMTLKQYFPVSLVLSGIFKIMQDLFGLRFEE 415
             F  +      DL YY+ + E+ + ++D  +LK+YFP+ +V  G+  I Q+L GL FE+
Sbjct: 348 RGFAYDGKINAWDLHYYMTQTEELKYSVDQESLKEYFPIEVVTEGLLSIYQELLGLSFEQ 407

Query: 416 VIDAEVWHYDVKLYSVFDLNSGELIGYFFLDLYTREGKYIHTCVVALQNSALLYNGTRQI 475
           V DA VW+  V LY+V D  +GE++G F+LDLY REGKY H     LQ   LL +G+R +
Sbjct: 408 VADAHVWNKSVSLYTVKDKATGEVLGQFYLDLYPREGKYNHAACFGLQPGCLLPDGSRMM 467

Query: 476 PVALLISQLEKDVGGHAGLMRFTEVVNLFHEFGHVVQHICNRASFTRISGLRVDPDFVEI 535
            VA L+    + + G   L+R  EV   FHEFGHV+  IC +  F R SG  V+ DFVE+
Sbjct: 468 SVAALVVNFSQPIAGRPSLLRHDEVRTYFHEFGHVMHQICAQTDFARFSGTNVETDFVEV 527

Query: 536 PAQILENWCYESVSLKLLSGFHQDITVPIKDEVCESLKKWRHSFSALKLKQEVLYCLFDQ 595
           P+Q+LENW ++  SL+ LS  ++D   PI DE+ E L   R   + L   ++++    DQ
Sbjct: 528 PSQMLENWVWDIDSLRKLSKHYRD-GHPITDELLEKLVASRLVNTGLLTLRQIVLSKVDQ 587

Query: 596 IIHCAENVDIVELFKHLHSKVMLGLPMLEGTNPASCFPCSAIGYEAACYSRVWSEVFSAD 655
            +H   ++D    +    +++ LG+    GTN  + F   A GY+   Y  +WSEVFS D
Sbjct: 588 SLHTNASLDAASEYAKYCTEI-LGVAATPGTNMPATFGHLAGGYDGQYYGYLWSEVFSMD 647

Query: 656 IFVSKFRGD-LLNQHTGLQFRNKVLAPGGAKEPIDILSDFLGREPSIQAFI 698
           +F S FR + ++N   G+++RN +L PGG+ + +D+L +FL REP+ +AF+
Sbjct: 648 MFHSCFRKEGIMNPEVGMKYRNLILKPGGSLDGMDMLQNFLQREPNQKAFL 696

BLAST of Lag0004695 vs. ExPASy Swiss-Prot
Match: P42676 (Neurolysin, mitochondrial OS=Rattus norvegicus OX=10116 GN=Nln PE=1 SV=1)

HSP 1 Score: 433.0 bits (1112), Expect = 6.7e-120
Identity = 244/651 (37.48%), Postives = 387/651 (59.45%), Query Frame = 0

Query: 56  LRVNLSPSEILNLADRIIAKSKKVHDAVASVPPNKVTYSNVISPLADLEAEQFPLVQSCV 115
           LR +LSP +I    +++IA++K+V+D V ++   +VTY N +  LAD+E           
Sbjct: 48  LRWDLSPEQIKTRTEQLIAQTKQVYDTVGTIALKEVTYENCLQVLADIEVTYIVERTMLD 107

Query: 116 FPKLISTSDDVRKASAEAERRIDAHVQMCSKRENVY-RVVKAFSTRG-EQTSAEQKCFIQ 175
           FP+ +S+  +VR AS EA++++       S RE+V+ R+V    T   E+   E + +++
Sbjct: 108 FPQHVSSDREVRAASTEADKKLSRFDIEMSMREDVFQRIVHLQETCDLEKIKPEARRYLE 167

Query: 176 CLVRDFERNGLNLTSTKREELLRLRVQIEELSLRYIQNLNDDGTFLPLSEAELDGLPKEF 235
             ++  +RNGL+L+   R E+  ++ ++ EL + + +NLN+D T L  S+AEL  LP +F
Sbjct: 168 KSIKMGKRNGLHLSEHIRNEIKSMKKRMSELCIDFNKNLNEDDTSLVFSKAELGALPDDF 227

Query: 236 FESLDKTENGKFKVILRSHHIAAVLEHCKVGTTRRMVAMAYGKRCGEVNLSILENLVLLR 295
            +SL+KT+  K+KV L+  H   V++ C V  TRR + MA+  RC + N +IL+ L+ LR
Sbjct: 228 IDSLEKTDEDKYKVTLKYPHYFPVMKKCCVPETRRKMEMAFHTRCKQENTAILQQLLPLR 287

Query: 296 HKFARLQGYSNYADYAVHYRMARSSSKVFEFLENISDSLTDLAAKELASLKDLKKQEEGE 355
            + A+L GY+ +AD+ +    A+S+S+V  FL+++S  L  L   E   +  LKK+E  E
Sbjct: 288 AQVAKLLGYNTHADFVLELNTAKSTSRVAAFLDDLSQKLKPLGEAEREFILSLKKKECEE 347

Query: 356 SPFGIE------DLLYYVKKVEDQELNLDFMTLKQYFPVSLVLSGIFKIMQDLFGLRFEE 415
             F  +      DL YY+ + E+ + ++D  +LK+YFP+ +V  G+  I Q+L GL FE+
Sbjct: 348 RGFEYDGKINAWDLHYYMTQTEELKYSVDQESLKEYFPIEVVTEGLLSIYQELLGLSFEQ 407

Query: 416 VIDAEVWHYDVKLYSVFDLNSGELIGYFFLDLYTREGKYIHTCVVALQNSALLYNGTRQI 475
           V DA VW+  V LY+V D  +GE++G F+LDLY REGKY H     LQ   LL +G+R +
Sbjct: 408 VPDAHVWNKSVSLYTVKDKATGEVLGQFYLDLYPREGKYNHAACFGLQPGCLLPDGSRMM 467

Query: 476 PVALLISQLEKDVGGHAGLMRFTEVVNLFHEFGHVVQHICNRASFTRISGLRVDPDFVEI 535
            VA L+    + V G   L+R  EV   FHEFGHV+  IC +  F R SG  V+ DFVE+
Sbjct: 468 SVAALVVNFSQPVAGRPSLLRHDEVRTYFHEFGHVMHQICAQTDFARFSGTNVETDFVEV 527

Query: 536 PAQILENWCYESVSLKLLSGFHQDITVPIKDEVCESLKKWRHSFSALKLKQEVLYCLFDQ 595
           P+Q+LENW ++  SL+ LS  ++D   PI DE+ E L   R   + L   ++++    DQ
Sbjct: 528 PSQMLENWVWDVDSLRKLSKHYKD-GHPITDELLEKLVASRLVNTGLLTLRQIVLSKVDQ 587

Query: 596 IIHCAENVDIVELFKHLHSKVMLGLPMLEGTNPASCFPCSAIGYEAACYSRVWSEVFSAD 655
            +H    +D    +    +++ LG+    GTN  + F   A GY+   Y  +WSEVFS D
Sbjct: 588 SLHTNATLDAASEYAKYCTEI-LGVAATPGTNMPATFGHLAGGYDGQYYGYLWSEVFSMD 647

Query: 656 IFVSKFRGD-LLNQHTGLQFRNKVLAPGGAKEPIDILSDFLGREPSIQAFI 698
           +F S F+ + ++N   G+++RN +L PGG+ + +D+L +FL REP+ +AF+
Sbjct: 648 MFHSCFKKEGIMNPEVGMKYRNLILKPGGSLDGMDMLQNFLQREPNQKAFL 696

BLAST of Lag0004695 vs. ExPASy Swiss-Prot
Match: Q02038 (Neurolysin, mitochondrial OS=Sus scrofa OX=9823 GN=NLN PE=1 SV=1)

HSP 1 Score: 432.2 bits (1110), Expect = 1.1e-119
Identity = 241/651 (37.02%), Postives = 386/651 (59.29%), Query Frame = 0

Query: 56  LRVNLSPSEILNLADRIIAKSKKVHDAVASVPPNKVTYSNVISPLADLEAEQFPLVQSCV 115
           LR +LSP +I    + +IA++K+V+D +  +   +VTY N +  LAD+E +         
Sbjct: 48  LRWDLSPEQIKRRTEELIAQTKQVYDDIGMLDIEEVTYENCLQALADVEVKYIVERTMLD 107

Query: 116 FPKLISTSDDVRKASAEAERRIDAHVQMCSKRENVY-RVVKAFSTRG-EQTSAEQKCFIQ 175
           FP+ +S+  +VR AS EA++R+       S RE+++ R+V+   T    +   E + +++
Sbjct: 108 FPQHVSSDKEVRAASTEADKRLSRFDIEMSMREDIFLRIVRLKETCDLGKIKPEARRYLE 167

Query: 176 CLVRDFERNGLNLTSTKREELLRLRVQIEELSLRYIQNLNDDGTFLPLSEAELDGLPKEF 235
             V+  +RNGL+L    + E+  ++ ++ EL + + +NLN+D TFL  S+AEL  LP +F
Sbjct: 168 KSVKMGKRNGLHLPEQVQNEIKAMKKRMSELCIDFNKNLNEDDTFLVFSKAELGALPDDF 227

Query: 236 FESLDKTENGKFKVILRSHHIAAVLEHCKVGTTRRMVAMAYGKRCGEVNLSILENLVLLR 295
            +SL+KT++ K+K+ L+  H   V++ C +  TRR + MA+  RC E N  IL+ L+ LR
Sbjct: 228 IDSLEKTDDNKYKITLKYPHYFPVMKKCCIPETRRKMEMAFNTRCKEENTIILQELLPLR 287

Query: 296 HKFARLQGYSNYADYAVHYRMARSSSKVFEFLENISDSLTDLAAKELASLKDLKKQEEGE 355
            K A+L GYS +AD+ +    A+S+  V  FL+++S  L  L   E   + +LKK+E  E
Sbjct: 288 AKVAKLLGYSTHADFVLEMNTAKSTHHVTAFLDDLSQKLKPLGEAEREFILNLKKKECEE 347

Query: 356 SPFGIE------DLLYYVKKVEDQELNLDFMTLKQYFPVSLVLSGIFKIMQDLFGLRFEE 415
             F  +      DL YY+ + E+ + ++D   LK+YFP+ +V  G+  I Q+L GL FE+
Sbjct: 348 KGFEYDGKINAWDLHYYMTQTEELKYSVDQEILKEYFPIEVVTEGLLNIYQELLGLSFEQ 407

Query: 416 VIDAEVWHYDVKLYSVFDLNSGELIGYFFLDLYTREGKYIHTCVVALQNSALLYNGTRQI 475
           V DA VW+  V LY+V D  +GE++G F+LDLY REGKY H     LQ   LL +G+R +
Sbjct: 408 VTDAHVWNKSVTLYTVKDKATGEVLGQFYLDLYPREGKYNHAACFGLQPGCLLPDGSRMM 467

Query: 476 PVALLISQLEKDVGGHAGLMRFTEVVNLFHEFGHVVQHICNRASFTRISGLRVDPDFVEI 535
            VA L+    +   G   L+R  EV   FHEFGHV+  IC +  F R SG  V+ DFVE+
Sbjct: 468 SVAALVVNFSQPRAGRPSLLRHDEVRTYFHEFGHVMHQICAQTDFARFSGTNVETDFVEV 527

Query: 536 PAQILENWCYESVSLKLLSGFHQDITVPIKDEVCESLKKWRHSFSALKLKQEVLYCLFDQ 595
           P+Q+LENW +++ SL+ LS  ++D + PI D++ E L   R   + L   ++++    DQ
Sbjct: 528 PSQMLENWVWDTDSLRRLSKHYKDGS-PITDDLLEKLVASRLVNTGLLTLRQIVLSKVDQ 587

Query: 596 IIHCAENVDIVELFKHLHSKVMLGLPMLEGTNPASCFPCSAIGYEAACYSRVWSEVFSAD 655
            +H   ++D    +    +++ LG+    GTN  + F   A GY+   Y  +WSEVFS D
Sbjct: 588 SLHTNTSLDAASEYAKYCTEI-LGVAATPGTNMPATFGHLAGGYDGQYYGYLWSEVFSMD 647

Query: 656 IFVSKFRGD-LLNQHTGLQFRNKVLAPGGAKEPIDILSDFLGREPSIQAFI 698
           +F S F+ + ++N   G+++RN +L PGG+ + +D+L +FL REP+ +AF+
Sbjct: 648 MFYSCFKKEGIMNPEVGMKYRNLILKPGGSLDGMDMLQNFLKREPNQKAFL 696

BLAST of Lag0004695 vs. ExPASy TrEMBL
Match: A0A6J1K568 (probable thimet oligopeptidase OS=Cucurbita maxima OX=3661 GN=LOC111491781 PE=3 SV=1)

HSP 1 Score: 1323.5 bits (3424), Expect = 0.0e+00
Identity = 666/705 (94.47%), Postives = 686/705 (97.30%), Query Frame = 0

Query: 1   MTEIQGNNSEKMDKRKSQGKLLVFTGGAALLAVAVNLAIVAISRRKKKKDLPGFELRVNL 60
           MTEI+G NSEKMDKRKS+ +LLVFTGGAALLAVAVNLAIVAIS RKKKK+LPGFELRVNL
Sbjct: 1   MTEIEG-NSEKMDKRKSRSQLLVFTGGAALLAVAVNLAIVAISNRKKKKELPGFELRVNL 60

Query: 61  SPSEILNLADRIIAKSKKVHDAVASVPPNKVTYSNVISPLADLEAEQFPLVQSCVFPKLI 120
           S SEILNLADRIIAKSKKVHDAVASVPPNKVTYSNVISPLADLEAEQFP VQSCVFPKLI
Sbjct: 61  SASEILNLADRIIAKSKKVHDAVASVPPNKVTYSNVISPLADLEAEQFPQVQSCVFPKLI 120

Query: 121 STSDDVRKASAEAERRIDAHVQMCSKRENVYRVVKAFSTRGEQTSAEQKCFIQCLVRDFE 180
           STSDDVRKASAEAERRIDAHVQMCSKRE+VYRVVKAFSTRGEQTSAEQKCFIQCL+RDFE
Sbjct: 121 STSDDVRKASAEAERRIDAHVQMCSKREDVYRVVKAFSTRGEQTSAEQKCFIQCLIRDFE 180

Query: 181 RNGLNLTSTKREELLRLRVQIEELSLRYIQNLNDDGTFLPLSEAELDGLPKEFFESLDKT 240
           RNGLNLTS KREELLRLRVQIEELSLRYIQNLNDDGTF+PLSEAELDGLPKEFFESLDK 
Sbjct: 181 RNGLNLTSPKREELLRLRVQIEELSLRYIQNLNDDGTFIPLSEAELDGLPKEFFESLDKN 240

Query: 241 ENGKFKVILRSHHIAAVLEHCKVGTTRRMVAMAYGKRCGEVNLSILENLVLLRHKFARLQ 300
           ENG+FKV +RSHH AA+LEHCKVGTTRRMVAM YGKRCGEVNLSILENLV LRHKFARLQ
Sbjct: 241 ENGQFKVTMRSHHTAAILEHCKVGTTRRMVAMTYGKRCGEVNLSILENLVHLRHKFARLQ 300

Query: 301 GYSNYADYAVHYRMARSSSKVFEFLENISDSLTDLAAKELASLKDLKKQEEGESPFGIED 360
           GYSNYADYAVHYRMARSS+KVFEFLE+ISDSLTD+AAKELASLKDLKKQEEGESPFGIED
Sbjct: 301 GYSNYADYAVHYRMARSSAKVFEFLESISDSLTDVAAKELASLKDLKKQEEGESPFGIED 360

Query: 361 LLYYVKKVEDQELNLDFMTLKQYFPVSLVLSGIFKIMQDLFGLRFEEVIDAEVWHYDVKL 420
           LLYYVKKVEDQE NLDF+TLKQYFPVSLVLSGIFKIMQDLFGLRFEEVIDAEVWH DVKL
Sbjct: 361 LLYYVKKVEDQEFNLDFVTLKQYFPVSLVLSGIFKIMQDLFGLRFEEVIDAEVWHDDVKL 420

Query: 421 YSVFDLNSGELIGYFFLDLYTREGKYIHTCVVALQNSALLYNGTRQIPVALLISQLEKDV 480
           YSVFDLNSGELIGYFFLDLYTREGKYIHTCVVALQNSALL NGTRQIPVALLISQL+KDV
Sbjct: 421 YSVFDLNSGELIGYFFLDLYTREGKYIHTCVVALQNSALLSNGTRQIPVALLISQLQKDV 480

Query: 481 GGHAGLMRFTEVVNLFHEFGHVVQHICNRASFTRISGLRVDPDFVEIPAQILENWCYESV 540
            GHAGLMRFTEVVNLFHEFGHVVQHICN ASFTRISGLRVD DFVEIPAQILENWCYESV
Sbjct: 481 DGHAGLMRFTEVVNLFHEFGHVVQHICNHASFTRISGLRVDSDFVEIPAQILENWCYESV 540

Query: 541 SLKLLSGFHQDITVPIKDEVCESLKKWRHSFSALKLKQEVLYCLFDQIIHCAENVDIVEL 600
           SLKLLSGFHQDITVPIKDEVCESLKKWRHSFSALKLKQEVLYCLFDQIIHCAENVDI+EL
Sbjct: 541 SLKLLSGFHQDITVPIKDEVCESLKKWRHSFSALKLKQEVLYCLFDQIIHCAENVDIIEL 600

Query: 601 FKHLHSKVMLGLPMLEGTNPASCFPCSAIGYEAACYSRVWSEVFSADIFVSKFRGDLLNQ 660
           FKHLHSKVMLGLPMLEG+NPASCFPCSAIGYEAACYSRVWSEVF+ADIFVSKFRGDLLNQ
Sbjct: 601 FKHLHSKVMLGLPMLEGSNPASCFPCSAIGYEAACYSRVWSEVFAADIFVSKFRGDLLNQ 660

Query: 661 HTGLQFRNKVLAPGGAKEPIDILSDFLGREPSIQAFIDTKAEYSL 706
           H GLQFRNKVL PGGAKEPID+LSDFLGREPSIQAFID+KAEYSL
Sbjct: 661 HIGLQFRNKVLVPGGAKEPIDVLSDFLGREPSIQAFIDSKAEYSL 704

BLAST of Lag0004695 vs. ExPASy TrEMBL
Match: A0A6J1HCG5 (probable thimet oligopeptidase OS=Cucurbita moschata OX=3662 GN=LOC111462816 PE=3 SV=1)

HSP 1 Score: 1315.8 bits (3404), Expect = 0.0e+00
Identity = 663/705 (94.04%), Postives = 685/705 (97.16%), Query Frame = 0

Query: 1   MTEIQGNNSEKMDKRKSQGKLLVFTGGAALLAVAVNLAIVAISRRKKKKDLPGFELRVNL 60
           M EI+G NSEKMDKRKS+ +LLVFTGGAALLAVAVNLAIVAIS RKKKK+LPGFELRVNL
Sbjct: 1   MKEIEG-NSEKMDKRKSRSQLLVFTGGAALLAVAVNLAIVAISNRKKKKELPGFELRVNL 60

Query: 61  SPSEILNLADRIIAKSKKVHDAVASVPPNKVTYSNVISPLADLEAEQFPLVQSCVFPKLI 120
           S SEILNLADRIIAKSKKVHDAVASVPPNKVTYSNVISPLADLEAEQFP VQSCVFPKLI
Sbjct: 61  SASEILNLADRIIAKSKKVHDAVASVPPNKVTYSNVISPLADLEAEQFPQVQSCVFPKLI 120

Query: 121 STSDDVRKASAEAERRIDAHVQMCSKRENVYRVVKAFSTRGEQTSAEQKCFIQCLVRDFE 180
           STSDDVRKASAEAE RIDAHVQMCSKRE+VYRVVKAFSTRGEQTSAEQKCFIQCLVRDFE
Sbjct: 121 STSDDVRKASAEAEWRIDAHVQMCSKREDVYRVVKAFSTRGEQTSAEQKCFIQCLVRDFE 180

Query: 181 RNGLNLTSTKREELLRLRVQIEELSLRYIQNLNDDGTFLPLSEAELDGLPKEFFESLDKT 240
           RNGLNLTS+KR+ELLRL VQIEELSLRYIQNLNDDGTF+PLSEAELDGLPKEFFESLDK 
Sbjct: 181 RNGLNLTSSKRKELLRLGVQIEELSLRYIQNLNDDGTFIPLSEAELDGLPKEFFESLDKN 240

Query: 241 ENGKFKVILRSHHIAAVLEHCKVGTTRRMVAMAYGKRCGEVNLSILENLVLLRHKFARLQ 300
           ENG+FKV +RSHH AA+LEHCKVG TRRMVAM YGKRCGEVNLSILENLV LRHKFARLQ
Sbjct: 241 ENGQFKVTMRSHHTAAILEHCKVGMTRRMVAMTYGKRCGEVNLSILENLVHLRHKFARLQ 300

Query: 301 GYSNYADYAVHYRMARSSSKVFEFLENISDSLTDLAAKELASLKDLKKQEEGESPFGIED 360
           GYSNYADYAVHYRMARSS+KVFEFLE+ISDSLTD+AAKELASLKDLKKQEEGESPFGIED
Sbjct: 301 GYSNYADYAVHYRMARSSAKVFEFLESISDSLTDVAAKELASLKDLKKQEEGESPFGIED 360

Query: 361 LLYYVKKVEDQELNLDFMTLKQYFPVSLVLSGIFKIMQDLFGLRFEEVIDAEVWHYDVKL 420
           LLYYVKKVEDQE NLDF+TLKQYFPVSLVLSGIFKIMQDLFGLRFEEVIDAEVWH DVKL
Sbjct: 361 LLYYVKKVEDQEFNLDFVTLKQYFPVSLVLSGIFKIMQDLFGLRFEEVIDAEVWHGDVKL 420

Query: 421 YSVFDLNSGELIGYFFLDLYTREGKYIHTCVVALQNSALLYNGTRQIPVALLISQLEKDV 480
           YSVFDLNSGELIGYFFLDLYTREGKYIHTCVVALQNSALL NGTRQIPVALLISQL+KDV
Sbjct: 421 YSVFDLNSGELIGYFFLDLYTREGKYIHTCVVALQNSALLSNGTRQIPVALLISQLQKDV 480

Query: 481 GGHAGLMRFTEVVNLFHEFGHVVQHICNRASFTRISGLRVDPDFVEIPAQILENWCYESV 540
            GHAGLMRFTEVVNLFHEFGHVVQHICN ASFTRISGLRVDPDFVEIPAQILENWCYESV
Sbjct: 481 DGHAGLMRFTEVVNLFHEFGHVVQHICNHASFTRISGLRVDPDFVEIPAQILENWCYESV 540

Query: 541 SLKLLSGFHQDITVPIKDEVCESLKKWRHSFSALKLKQEVLYCLFDQIIHCAENVDIVEL 600
           SLKLLSGFHQDITVPIKDEVCESLKKWRHSFSALKLKQEVLYCLFDQIIHCAENVDI+EL
Sbjct: 541 SLKLLSGFHQDITVPIKDEVCESLKKWRHSFSALKLKQEVLYCLFDQIIHCAENVDIIEL 600

Query: 601 FKHLHSKVMLGLPMLEGTNPASCFPCSAIGYEAACYSRVWSEVFSADIFVSKFRGDLLNQ 660
           FKHLHSKVMLGLPMLEG+NPASCFPCSAIG+EAACYSRVWSEVF+ADIFVSKFRGDLLNQ
Sbjct: 601 FKHLHSKVMLGLPMLEGSNPASCFPCSAIGFEAACYSRVWSEVFAADIFVSKFRGDLLNQ 660

Query: 661 HTGLQFRNKVLAPGGAKEPIDILSDFLGREPSIQAFIDTKAEYSL 706
           H GLQFRNKVLAPGGAKEPID+LSDFLGREPSIQAFID+KAEYSL
Sbjct: 661 HIGLQFRNKVLAPGGAKEPIDVLSDFLGREPSIQAFIDSKAEYSL 704

BLAST of Lag0004695 vs. ExPASy TrEMBL
Match: A0A0A0LMX1 (Peptidase_M3 domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_2G350370 PE=3 SV=1)

HSP 1 Score: 1311.2 bits (3392), Expect = 0.0e+00
Identity = 655/705 (92.91%), Postives = 684/705 (97.02%), Query Frame = 0

Query: 1   MTEIQGNNSEKMDKRKSQGKLLVFTGGAALLAVAVNLAIVAISRRKKKKDLPGFELRVNL 60
           MTEIQG N++KM KRK+Q KLLVFTGGAALLAVAVNLAIVAI +RKKKK+LPGFELR NL
Sbjct: 1   MTEIQG-NTQKMYKRKTQSKLLVFTGGAALLAVAVNLAIVAICKRKKKKELPGFELRPNL 60

Query: 61  SPSEILNLADRIIAKSKKVHDAVASVPPNKVTYSNVISPLADLEAEQFPLVQSCVFPKLI 120
           S SEILNLAD+IIAKSKKVHDAVASVPPNKVTYSNVISPLADLEAEQFPLVQSCVFPKLI
Sbjct: 61  SASEILNLADKIIAKSKKVHDAVASVPPNKVTYSNVISPLADLEAEQFPLVQSCVFPKLI 120

Query: 121 STSDDVRKASAEAERRIDAHVQMCSKRENVYRVVKAFSTRGEQTSAEQKCFIQCLVRDFE 180
           STSDDVR ASAEAERRIDAH QMCSKRE+VYRVVKAFS RGEQTSAEQKCFIQCLVRDFE
Sbjct: 121 STSDDVRAASAEAERRIDAHAQMCSKREDVYRVVKAFSARGEQTSAEQKCFIQCLVRDFE 180

Query: 181 RNGLNLTSTKREELLRLRVQIEELSLRYIQNLNDDGTFLPLSEAELDGLPKEFFESLDKT 240
           RNGLNLT++KR+ELLRLRVQIEELSLRYIQNLNDDGTF+P SEAELDGLPKEFFESLDKT
Sbjct: 181 RNGLNLTTSKRKELLRLRVQIEELSLRYIQNLNDDGTFIPFSEAELDGLPKEFFESLDKT 240

Query: 241 ENGKFKVILRSHHIAAVLEHCKVGTTRRMVAMAYGKRCGEVNLSILENLVLLRHKFARLQ 300
           ENGKFKV++RSHH A VLEHCKVGTTRRMVAMAYGKRCGEVNLSILENLV LRHKFARLQ
Sbjct: 241 ENGKFKVVMRSHHTAVVLEHCKVGTTRRMVAMAYGKRCGEVNLSILENLVHLRHKFARLQ 300

Query: 301 GYSNYADYAVHYRMARSSSKVFEFLENISDSLTDLAAKELASLKDLKKQEEGESPFGIED 360
           GYSNYADYAVHYRMARSS+KVFEFLENISDS+TDLAAKELASLK+LKKQEEGESPFGIED
Sbjct: 301 GYSNYADYAVHYRMARSSAKVFEFLENISDSITDLAAKELASLKNLKKQEEGESPFGIED 360

Query: 361 LLYYVKKVEDQELNLDFMTLKQYFPVSLVLSGIFKIMQDLFGLRFEEVIDAEVWHYDVKL 420
           LLYYVK+ EDQE NLDF+T+KQYFPVSLVLSGIFKIMQDLFGLRFEEVIDAEVWHYDVKL
Sbjct: 361 LLYYVKRAEDQEFNLDFVTVKQYFPVSLVLSGIFKIMQDLFGLRFEEVIDAEVWHYDVKL 420

Query: 421 YSVFDLNSGELIGYFFLDLYTREGKYIHTCVVALQNSALLYNGTRQIPVALLISQLEKDV 480
           YSVFDLNSGELIGYFFLDLYTRE KYIHTCVVALQ+SALL NGTRQIPVALL+SQL+ DV
Sbjct: 421 YSVFDLNSGELIGYFFLDLYTREEKYIHTCVVALQSSALLSNGTRQIPVALLLSQLQNDV 480

Query: 481 GGHAGLMRFTEVVNLFHEFGHVVQHICNRASFTRISGLRVDPDFVEIPAQILENWCYESV 540
            GHAGLMRFTEVVNLFHEFGHVVQH+CNRA FTRISGLR+DPDFVEIPAQILENWCYESV
Sbjct: 481 DGHAGLMRFTEVVNLFHEFGHVVQHVCNRAPFTRISGLRLDPDFVEIPAQILENWCYESV 540

Query: 541 SLKLLSGFHQDITVPIKDEVCESLKKWRHSFSALKLKQEVLYCLFDQIIHCAENVDIVEL 600
           SLKLLSGFHQDITVPIKDEVCESLKKWRHSFSALKLKQE+LYCLFDQIIHCAENVDI+EL
Sbjct: 541 SLKLLSGFHQDITVPIKDEVCESLKKWRHSFSALKLKQEILYCLFDQIIHCAENVDIIEL 600

Query: 601 FKHLHSKVMLGLPMLEGTNPASCFPCSAIGYEAACYSRVWSEVFSADIFVSKFRGDLLNQ 660
           FKHLHSKVMLGLPMLEGTNPASCFPCSAIGYEAACYSRVWSEVFSADIFVSKFRG+LLNQ
Sbjct: 601 FKHLHSKVMLGLPMLEGTNPASCFPCSAIGYEAACYSRVWSEVFSADIFVSKFRGNLLNQ 660

Query: 661 HTGLQFRNKVLAPGGAKEPIDILSDFLGREPSIQAFIDTKAEYSL 706
           H GLQFRNKVLAPGGAKEPID+LSDFLGREPSIQAFID+KAEYSL
Sbjct: 661 HIGLQFRNKVLAPGGAKEPIDVLSDFLGREPSIQAFIDSKAEYSL 704

BLAST of Lag0004695 vs. ExPASy TrEMBL
Match: A0A1S3BAC2 (probable thimet oligopeptidase isoform X1 OS=Cucumis melo OX=3656 GN=LOC103487749 PE=3 SV=1)

HSP 1 Score: 1307.4 bits (3382), Expect = 0.0e+00
Identity = 657/705 (93.19%), Postives = 683/705 (96.88%), Query Frame = 0

Query: 1   MTEIQGNNSEKMDKRKSQGKLLVFTGGAALLAVAVNLAIVAISRRKKKKDLPGFELRVNL 60
           MTEIQG N++KM KRK+Q KLLVFTGGAALLAVAVNLAIV I +RKKKK+LPGFELRVNL
Sbjct: 1   MTEIQG-NTQKMYKRKTQSKLLVFTGGAALLAVAVNLAIVVICKRKKKKELPGFELRVNL 60

Query: 61  SPSEILNLADRIIAKSKKVHDAVASVPPNKVTYSNVISPLADLEAEQFPLVQSCVFPKLI 120
           S +EILNLAD+IIAKSKKVHDAVASVPPNKVTYSNVISPLADLEAEQFPLVQSCVFPKLI
Sbjct: 61  SAAEILNLADKIIAKSKKVHDAVASVPPNKVTYSNVISPLADLEAEQFPLVQSCVFPKLI 120

Query: 121 STSDDVRKASAEAERRIDAHVQMCSKRENVYRVVKAFSTRGEQTSAEQKCFIQCLVRDFE 180
           STSDDVR ASAEAERRIDAHVQMCSKRE+VYRVVKAFS RGEQTSAEQKCFIQCLVRDFE
Sbjct: 121 STSDDVRAASAEAERRIDAHVQMCSKREDVYRVVKAFSARGEQTSAEQKCFIQCLVRDFE 180

Query: 181 RNGLNLTSTKREELLRLRVQIEELSLRYIQNLNDDGTFLPLSEAELDGLPKEFFESLDKT 240
           RNGLNLT++KREELLRLRVQIEELSLRYIQNLNDDGTF+P SE ELDGLPKEFFESLDKT
Sbjct: 181 RNGLNLTTSKREELLRLRVQIEELSLRYIQNLNDDGTFIPFSEVELDGLPKEFFESLDKT 240

Query: 241 ENGKFKVILRSHHIAAVLEHCKVGTTRRMVAMAYGKRCGEVNLSILENLVLLRHKFARLQ 300
           ENGKFKV++RSHH AAVLEHCKVGTTRRMVAMAYGKRCGEVNLSILENLV LRHKFARLQ
Sbjct: 241 ENGKFKVVMRSHHTAAVLEHCKVGTTRRMVAMAYGKRCGEVNLSILENLVHLRHKFARLQ 300

Query: 301 GYSNYADYAVHYRMARSSSKVFEFLENISDSLTDLAAKELASLKDLKKQEEGESPFGIED 360
           GYSNYADYAVHYRMARSS+KVFEFLENISDSLTDLAAKELASLK+LKKQEEGESPFGIED
Sbjct: 301 GYSNYADYAVHYRMARSSAKVFEFLENISDSLTDLAAKELASLKNLKKQEEGESPFGIED 360

Query: 361 LLYYVKKVEDQELNLDFMTLKQYFPVSLVLSGIFKIMQDLFGLRFEEVIDAEVWHYDVKL 420
           LLYYVK+ EDQE NLDF+T+KQYFPVSLVLSGIFKIMQDLFGLRFEEVIDAEVWHYDVKL
Sbjct: 361 LLYYVKRAEDQEFNLDFVTVKQYFPVSLVLSGIFKIMQDLFGLRFEEVIDAEVWHYDVKL 420

Query: 421 YSVFDLNSGELIGYFFLDLYTREGKYIHTCVVALQNSALLYNGTRQIPVALLISQLEKDV 480
           YSVFDLNSGELIGYFFLDLY RE KYIHTCVVALQ+SALL NGTRQIPVALLISQL+ DV
Sbjct: 421 YSVFDLNSGELIGYFFLDLYAREEKYIHTCVVALQSSALLSNGTRQIPVALLISQLQNDV 480

Query: 481 GGHAGLMRFTEVVNLFHEFGHVVQHICNRASFTRISGLRVDPDFVEIPAQILENWCYESV 540
            GHAGLMRFTEVVNLFHEFGHVVQH+CNRASFTRISGLRVDPDFVEIPAQILENWCYESV
Sbjct: 481 DGHAGLMRFTEVVNLFHEFGHVVQHVCNRASFTRISGLRVDPDFVEIPAQILENWCYESV 540

Query: 541 SLKLLSGFHQDITVPIKDEVCESLKKWRHSFSALKLKQEVLYCLFDQIIHCAENVDIVEL 600
           SLKLLSGFHQDITVPIKDEVCESLKKWRHSFSALKLKQEVLYCLFDQIIHCAENVDI+EL
Sbjct: 541 SLKLLSGFHQDITVPIKDEVCESLKKWRHSFSALKLKQEVLYCLFDQIIHCAENVDIIEL 600

Query: 601 FKHLHSKVMLGLPMLEGTNPASCFPCSAIGYEAACYSRVWSEVFSADIFVSKFRGDLLNQ 660
           FKHLHSKVMLGLPMLEGTNPASCFP SAIGYEAACYSRVWSEVFSADIFVSKFRG+LLNQ
Sbjct: 601 FKHLHSKVMLGLPMLEGTNPASCFPSSAIGYEAACYSRVWSEVFSADIFVSKFRGNLLNQ 660

Query: 661 HTGLQFRNKVLAPGGAKEPIDILSDFLGREPSIQAFIDTKAEYSL 706
           + GLQFRNKVLAPGGAKEPID+LSDFLGREPSIQAFID+KAE SL
Sbjct: 661 YIGLQFRNKVLAPGGAKEPIDVLSDFLGREPSIQAFIDSKAECSL 704

BLAST of Lag0004695 vs. ExPASy TrEMBL
Match: A0A6J1BRD7 (probable thimet oligopeptidase isoform X1 OS=Momordica charantia OX=3673 GN=LOC111004893 PE=3 SV=1)

HSP 1 Score: 1298.9 bits (3360), Expect = 0.0e+00
Identity = 656/705 (93.05%), Postives = 678/705 (96.17%), Query Frame = 0

Query: 1   MTEIQGNNSEKMDKRKSQGKLLVFTGGAALLAVAVNLAIVAISRRKKKKDLPGFELRVNL 60
           MTEIQG  SEKMDKRK + +LLVFTGGAALLAVA NLA+ AI RRKKKK+LPG ++RVNL
Sbjct: 1   MTEIQG-KSEKMDKRKRESRLLVFTGGAALLAVAANLAVAAIIRRKKKKELPGCKVRVNL 60

Query: 61  SPSEILNLADRIIAKSKKVHDAVASVPPNKVTYSNVISPLADLEAEQFPLVQSCVFPKLI 120
           S SEILNLADRIIA SKKVHDAVASVPPNKVT+SNVISPLADLEAEQFPLVQSCVFPKL+
Sbjct: 61  SASEILNLADRIIANSKKVHDAVASVPPNKVTHSNVISPLADLEAEQFPLVQSCVFPKLV 120

Query: 121 STSDDVRKASAEAERRIDAHVQMCSKRENVYRVVKAFSTRGEQTSAEQKCFIQCLVRDFE 180
           STSDDVRKASAEAERRIDAHVQMCSKRE+VYRV+K+FSTR EQTSAEQKCFIQ LVRDFE
Sbjct: 121 STSDDVRKASAEAERRIDAHVQMCSKREDVYRVIKSFSTRYEQTSAEQKCFIQRLVRDFE 180

Query: 181 RNGLNLTSTKREELLRLRVQIEELSLRYIQNLNDDGTFLPLSEAELDGLPKEFFESLDKT 240
           RNGLNL+STKREEL RLRVQIEELSLRYIQNLNDDGTFLPLSEAELDGLP+EFFESLDKT
Sbjct: 181 RNGLNLSSTKREELERLRVQIEELSLRYIQNLNDDGTFLPLSEAELDGLPREFFESLDKT 240

Query: 241 ENGKFKVILRSHHIAAVLEHCKVGTTRRMVAMAYGKRCGEVNLSILENLVLLRHKFARLQ 300
           ENGKFKVI+RSHHIAAVLEHCKVG TRRMVA AYGKRCGEVNLSILENLV LRHKF+RLQ
Sbjct: 241 ENGKFKVIMRSHHIAAVLEHCKVGATRRMVATAYGKRCGEVNLSILENLVHLRHKFSRLQ 300

Query: 301 GYSNYADYAVHYRMARSSSKVFEFLENISDSLTDLAAKELASLKDLKKQEEGESPFGIED 360
           GYSNYADYAVHYRMARSSSKVFEFLENISD LTDLAAKEL SLKDLKKQEEGESPFGIED
Sbjct: 301 GYSNYADYAVHYRMARSSSKVFEFLENISDCLTDLAAKELDSLKDLKKQEEGESPFGIED 360

Query: 361 LLYYVKKVEDQELNLDFMTLKQYFPVSLVLSGIFKIMQDLFGLRFEEVIDAEVWHYDVKL 420
           LLYYVKKVEDQE NLDF+TLKQYFPVSLVLSGIFKI+QDLFGLRFEEVIDAEVWHYDVKL
Sbjct: 361 LLYYVKKVEDQEFNLDFVTLKQYFPVSLVLSGIFKIIQDLFGLRFEEVIDAEVWHYDVKL 420

Query: 421 YSVFDLNSGELIGYFFLDLYTREGKYIHTCVVALQNSALLYNGTRQIPVALLISQLEKDV 480
           YSVFDLNSGELIGYF+LD Y REGKYIHTCVVALQNSALL NGTRQIPVALLISQL+K V
Sbjct: 421 YSVFDLNSGELIGYFYLDPYAREGKYIHTCVVALQNSALLSNGTRQIPVALLISQLQKAV 480

Query: 481 GGHAGLMRFTEVVNLFHEFGHVVQHICNRASFTRISGLRVDPDFVEIPAQILENWCYESV 540
           GGH GLMRF+EVVNLFHEFGHVVQHICNRASFTRISGL VDPDFVEIPAQILENWCYESV
Sbjct: 481 GGHTGLMRFSEVVNLFHEFGHVVQHICNRASFTRISGLCVDPDFVEIPAQILENWCYESV 540

Query: 541 SLKLLSGFHQDITVPIKDEVCESLKKWRHSFSALKLKQEVLYCLFDQIIHCAENVDIVEL 600
           SLKLLSGFHQDITVPIKDEVCESLKKWRHSFSALKLKQEVLYCLFDQIIHCAENVDI+EL
Sbjct: 541 SLKLLSGFHQDITVPIKDEVCESLKKWRHSFSALKLKQEVLYCLFDQIIHCAENVDIIEL 600

Query: 601 FKHLHSKVMLGLPMLEGTNPASCFPCSAIGYEAACYSRVWSEVFSADIFVSKFRGDLLNQ 660
           FKHLHSKVMLGLPMLEGTNPASCFP SAIGYEAACYSRVWSEVFSADIFVSKFRGDLLNQ
Sbjct: 601 FKHLHSKVMLGLPMLEGTNPASCFPSSAIGYEAACYSRVWSEVFSADIFVSKFRGDLLNQ 660

Query: 661 HTGLQFRNKVLAPGGAKEPIDILSDFLGREPSIQAFIDTKAEYSL 706
           H GLQFRNKVLAPGGAKEPID+LSDFLGREPSIQAFIDTKAEYSL
Sbjct: 661 HIGLQFRNKVLAPGGAKEPIDVLSDFLGREPSIQAFIDTKAEYSL 704

BLAST of Lag0004695 vs. TAIR 10
Match: AT1G67690.1 (Zincin-like metalloproteases family protein )

HSP 1 Score: 875.9 bits (2262), Expect = 2.1e-254
Identity = 426/710 (60.00%), Postives = 562/710 (79.15%), Query Frame = 0

Query: 1   MTEIQGNNSE-KMDKRKSQGKLLVFTGGAALLAVAVNLAIVAISRRK---KKKDLPGFE- 60
           MTE +GN+ + +    K +  ++ FTG A LL +AV+ AI      K   KKK LPG + 
Sbjct: 1   MTENEGNDKKIEGSNPKKKLNVVTFTGAAGLLGLAVSFAIFTFKSHKQKSKKKGLPGCDT 60

Query: 61  LRVNLSPSEILNLADRIIAKSKKVHDAVASVPPNKVTYSNVISPLADLEAEQFPLVQSCV 120
           + VNLS  EIL+LA+ II KS +VHDAVA V  +K++Y NV+ PLA+LEA Q  L+Q CV
Sbjct: 61  VCVNLSAKEILDLAEEIIHKSTRVHDAVALVSLDKLSYENVVLPLAELEARQLSLIQCCV 120

Query: 121 FPKLISTSDDVRKASAEAERRIDAHVQMCSKRENVYRVVKAFSTRGEQTSAEQKCFIQCL 180
           FPK++S  D+VRKAS EAE++IDAH+  C KRE+VYR++K ++ +GE  S E KC++QCL
Sbjct: 121 FPKMLSPHDNVRKASTEAEQKIDAHILSCRKREDVYRIIKIYAAKGESISPEAKCYLQCL 180

Query: 181 VRDFERNGLNLTSTKREELLRLRVQIEELSLRYIQNLNDDGTFLPLSEAELDGLPKEFFE 240
           VRDFE NGLNLT+ KREE+ RL+ +I+ELSLRYIQNLN+D + L  +E EL GLP EF +
Sbjct: 181 VRDFEDNGLNLTAIKREEVERLKYEIDELSLRYIQNLNEDSSCLFFTEDELAGLPLEFLQ 240

Query: 241 SLDKTENGKFKVILRSHHIAAVLEHCKVGTTRRMVAMAYGKRCGEVNLSILENLVLLRHK 300
           +L+KT+N +FK+ L S H+AA+LE CK+  TR+ VAMAYGKRCG+ N+ +L+ LV  RH+
Sbjct: 241 NLEKTQNKEFKLTLESRHVAAILELCKIAKTRKTVAMAYGKRCGDTNIPVLQRLVQSRHR 300

Query: 301 FARLQGYSNYADYAVHYRMARSSSKVFEFLENISDSLTDLAAKELASLKDLKKQEEGESP 360
            A + GY+++ADYA+  RM+++S +V  FLE+IS SLTDLA +E + L+DLK++EEGE P
Sbjct: 301 LACVCGYAHFADYALDRRMSKTSMRVIRFLEDISSSLTDLAIREFSILEDLKRKEEGEIP 360

Query: 361 FGIEDLLYYVKKVEDQELNLDFMTLKQYFPVSLVLSGIFKIMQDLFGLRFEEVIDAEVWH 420
           FG+EDLLYY+K+VE+ + +LDF  ++QYFPV+LVLSGIFKI QDLFG++FEEV + +VW+
Sbjct: 361 FGVEDLLYYIKRVEELQFDLDFGDIRQYFPVNLVLSGIFKICQDLFGIKFEEVTEVDVWY 420

Query: 421 YDVKLYSVFDLNSGELIGYFFLDLYTREGKYIHTCVVALQNSALLYNGTRQIPVALLISQ 480
           +D++ ++VFD  SG+L+GYF+LD++TREGK  H+CVVALQN+AL  NG  QIPVALLI+Q
Sbjct: 421 HDIRAFAVFDSGSGKLLGYFYLDMFTREGKCNHSCVVALQNNALFSNGACQIPVALLIAQ 480

Query: 481 LEKDVGGHAGLMRFTEVVNLFHEFGHVVQHICNRASFTRISGLRVDPDFVEIPAQILENW 540
             KD  G A  + F++VVNLFHEFGHVVQHICNRASF R SGLRVDPDF EIP+Q+LENW
Sbjct: 481 FAKDGSGEAVPLGFSDVVNLFHEFGHVVQHICNRASFARFSGLRVDPDFREIPSQLLENW 540

Query: 541 CYESVSLKLLSGFHQDITVPIKDEVCESLKKWRHSFSALKLKQEVLYCLFDQIIHCAENV 600
           CYES +LKL+SG+ QDIT P+ DEVC++LK+WR+SFSALK  QE+LYCLFDQII+  ++ 
Sbjct: 541 CYESFTLKLISGYRQDITKPLVDEVCKTLKRWRYSFSALKSLQEILYCLFDQIIYSDDDA 600

Query: 601 DIVELFKHLHSKVMLGLPMLEGTNPASCFPCSAIGYEAACYSRVWSEVFSADIFVSKFRG 660
           D+++L + LH KVM+GLP++EGTNPASCFP + IG EA CYSR+WSEV++ADIF SKF  
Sbjct: 601 DLLQLIRSLHPKVMIGLPVVEGTNPASCFPRAVIGSEATCYSRLWSEVYAADIFASKFGD 660

Query: 661 DLLNQHTGLQFRNKVLAPGGAKEPIDILSDFLGREPSIQAFIDTKAEYSL 706
              N + GLQFR+KVLAPGG KEP+++L++FLGREPS QAFI ++  YSL
Sbjct: 661 GHPNLYAGLQFRDKVLAPGGGKEPMELLTNFLGREPSTQAFIASRTNYSL 710

BLAST of Lag0004695 vs. TAIR 10
Match: AT5G65620.1 (Zincin-like metalloproteases family protein )

HSP 1 Score: 209.5 bits (532), Expect = 8.6e-54
Identity = 173/616 (28.08%), Postives = 296/616 (48.05%), Query Frame = 0

Query: 117 PKLISTSDDVRKASAEAERRIDAHVQMCSKRENVYRVVKAF--STRGEQTSAEQKCFIQC 176
           P+L +  +DV+    + + R+        + + +Y   KA   S      S  ++  ++ 
Sbjct: 174 PELRAAIEDVQPEKVKFQLRL-------GQSKPIYNAFKAIRESPDWSSLSEARQRLVEA 233

Query: 177 LVRDFERNGLNLTSTKREELLRLRVQIEELSLRYIQNLND---DGTFLPLSEAELDGLPK 236
            +++    G+ L   KREE  ++  ++E+LS ++ +N+ D       L   + E++GLP 
Sbjct: 234 QIKEAVLIGIALDDEKREEFNKIEQELEKLSHKFSENVLDATKKFEKLITDKKEIEGLPP 293

Query: 237 EFF-----ESLDK------TENGKFKVILRSHHIAAVLEHCKVGTTRRMVAMAYGKRC-- 296
                    ++ K       ENG + + L +     V++H K    R  V  AY  R   
Sbjct: 294 SALGLFAQAAVSKGHENATAENGPWIITLDAPSYLPVMQHAKNRALREEVYRAYLSRASS 353

Query: 297 GEV-NLSILENLVLLRHKFARLQGYSNYADYAVHYRMARSSSKVFEFLENISDSLTDLAA 356
           G++ N +I++ ++ LR + A+L GY+NYA+ ++  +MA +  K  E LE +  +  D A 
Sbjct: 354 GDLDNTAIIDQILKLRLEKAKLLGYNNYAEVSMAMKMA-TVEKAAELLEKLRSASWDAAV 413

Query: 357 KELASLKDLKKQEEGESPFGIE--DLLYYVKKVEDQELNLDFMTLKQYFPVSLVLSGIFK 416
           +++  LK   K +       +   D  ++ +++ + + +++   L+ YF +  V+ G+F 
Sbjct: 414 QDMEDLKSFAKNQGAAESDSMTHWDTTFWSERLRESKYDINEEELRPYFSLPKVMDGLFS 473

Query: 417 IMQDLFGLRFEEVID-AEVWHYDVKLYSVFDLNSGELIGYFFLDLYTR----EGKYIHTC 476
           + + LFG+  E     A VW+ DV+ Y V D +SG  I YF+ D Y+R     G      
Sbjct: 474 LAKTLFGIDIEPADGLAPVWNNDVRFYRVKD-SSGNPIAYFYFDPYSRPSEKRGGAWMDE 533

Query: 477 VVALQNSALLYNGTRQIPVALLISQLEKDVGGHAGLMRFTEVVNLFHEFGHVVQHICNRA 536
           VV+          + ++PVA ++      VG    LM F EV  +FHEFGH +QH+  + 
Sbjct: 534 VVSRSRVMAQKGSSVRLPVAHMVCNQTPPVGDKPSLMTFREVETVFHEFGHALQHMLTKQ 593

Query: 537 SFTRISGLR-VDPDFVEIPAQILENWCYESVSLKLLSGFHQDITVPIKDEVCESLKKWR- 596
               ++G+R ++ D VE+P+Q +ENWCY   +L  ++  H +    + +EV + L   R 
Sbjct: 594 DEGLVAGIRNIEWDAVELPSQFMENWCYHRDTLMSIAK-HYETGETLPEEVYKKLLAART 653

Query: 597 ---HSFSALKLKQEVLYCLFDQIIHC----AENVDIVELFKHLHSKVMLGLPMLEGTNPA 656
               SFS  +LK    +   D  +H          I ++ + +  K  +  P+ E     
Sbjct: 654 FRAGSFSLRQLK----FASVDLELHTKYVPGGPESIYDVDQRVSVKTQVIPPLPEDRFLC 713

Query: 657 SCFPCSAIGYEAACYSRVWSEVFSADIFVSKFRGDLLN-----QHTGLQFRNKVLAPGGA 693
           S     A GY A  YS  W+EV SAD F S F    L+     + TG +FRN +LA GG 
Sbjct: 714 SFSHIFAGGYAAGYYSYKWAEVLSADAF-SAFEDAGLDDIKAVKETGQRFRNTILALGGG 773

BLAST of Lag0004695 vs. TAIR 10
Match: AT5G51540.1 (Zincin-like metalloproteases family protein )

HSP 1 Score: 209.1 bits (531), Expect = 1.1e-53
Identity = 151/590 (25.59%), Postives = 278/590 (47.12%), Query Frame = 0

Query: 124 DDVRKASAEAERRIDAHVQMCSKRENVYRVVKAFSTRGEQTSAEQKCFIQCLVRDFERNG 183
           ++  KA+ E    ++ ++   +    +Y  VK         + E       L  DFER G
Sbjct: 104 EEANKAAIE----MNDYLHHLNTNHTLYAAVKKAEQDSNLLTKEASRTAHHLRMDFERGG 163

Query: 184 LNLTSTKREELLRLRVQIEELSLRYIQNLNDD--------GTFLPLSEAEL--------D 243
           ++L   K +++  L   I +L   + +N+ DD        G+ +P     L         
Sbjct: 164 IHLDPEKLDKVNNLTTNIFQLCREFSENIADDPGHVDIFPGSRIPRHLHHLLNPTYRSTS 223

Query: 244 GLPKEFFESLDKTENGKFKVILRSHHIAAVLEHCKVGTTRRMVAMAYGKRCGEVNLSILE 303
           G  +    S  K++   F++      ++++L+       R+MV +  G      N  +LE
Sbjct: 224 GGSRGSTRSAHKSKQKGFRINTDPRTVSSILQWTSDEEVRKMVYI-QGNSVPHANHGVLE 283

Query: 304 NLVLLRHKFARLQGYSNYADYAVHYRMARSSSKVFEFLENISDSLTDLAAKELASLKDLK 363
            L+  RH+ +++ G ++YAD  V   +A+S   V  FL+ +S ++   A +E  +++D K
Sbjct: 284 KLIAARHELSQMMGCNSYADIMVEPNLAKSPKVVTSFLQELSKTVKPKADEEFIAIRDFK 343

Query: 364 KQEEGESPFGIE--DLLYYVKKVEDQELNLDFMTLKQYFPVSLVLSGIFKIMQDLFGLRF 423
           +++ G     +E  D  YY   ++    ++D   +  YFP+   + G+  +++ LFG  F
Sbjct: 344 REKCGNPSAELEPWDETYYTSMMKSSINDVDTAVVASYFPLPQCIEGLKVLVESLFGATF 403

Query: 424 EEV--IDAEVWHYDVKLYSVFDLNSGELIGYFFLDLYTREGKYIHTCVVALQNSALLYNG 483
             +     E WH +V   S+   + G+L GY +LDLY+R+GKY      A++    +   
Sbjct: 404 HTIPLAPGESWHPNVVKLSLHHPDEGDL-GYLYLDLYSRKGKYPGCASFAIRGGRKISET 463

Query: 484 TRQIPVALLISQLEKDVGGHAGLMRFTEVVNLFHEFGHVVQHICNRASFTRISGLRVDPD 543
             Q+PV  L+    +        +  +EV  LFHEFGH +  + +R  +   SG RV  D
Sbjct: 464 EYQLPVIALVCNFSRACDSSIVKLNHSEVEVLFHEFGHALHSLLSRTDYQHFSGTRVALD 523

Query: 544 FVEIPAQILENWCYESVSLKLLSGFHQDITVPIKDEVCESLKKWRHSFSALKLKQEVLYC 603
             E+P+ + E + ++   LK  +  H      I +++  SL+  R+ F+A +++++V Y 
Sbjct: 524 LAEMPSNLFEYYAWDYRLLKRFAR-HYSTGETIPEKLVNSLQGARNMFAATEMQRQVFYA 583

Query: 604 LFDQIIHCAENVDIVELFKHLHSKVM---LGLPMLEGTNPASCFPCSAIGYEAACYSRVW 663
           L DQ++   E  +      HL +++         +EGT+    F    + Y A  YS ++
Sbjct: 584 LIDQMLF-GEQPETARDVSHLVAELKRQHTSWNHVEGTHWYIRF-SHLLNYGAGYYSYLY 643

Query: 664 SEVFSADIFVSKFRGDLLNQHTGLQFRNKVLAPGGAKEPIDILSDFLGRE 691
           ++ F++ I+ S    D L+ +TG   R K    GGAK+P ++L+D  G+E
Sbjct: 644 AKCFASTIWQSICEEDPLSLNTGTLLREKFFKHGGAKDPAELLTDLAGKE 684

BLAST of Lag0004695 vs. TAIR 10
Match: AT5G10540.1 (Zincin-like metalloproteases family protein )

HSP 1 Score: 199.5 bits (506), Expect = 8.9e-51
Identity = 162/621 (26.09%), Postives = 292/621 (47.02%), Query Frame = 0

Query: 118 KLISTSDDVRKASAEAERRIDAHVQMCSKRENVYRVVKAF--STRGEQTSAEQKCFIQCL 177
           K +  + ++R A  E +           + + +Y   KA   S      S  ++  ++  
Sbjct: 80  KAVKDTPELRAAIEEVQPEKVKFQLRLGQSKPIYNAFKAIRESPDWNSLSEARQRLVEAQ 139

Query: 178 VRDFERNGLNLTSTKREELLRLRVQIEELSLRYIQNLND---DGTFLPLSEAELDGLPKE 237
           +++   +G+ L   KREE  ++  ++E+LS ++ +N+ D       L   + E++GLP  
Sbjct: 140 IKEAVLSGIALEDDKREEFNKIEQELEKLSHKFSENVLDATKKFEKLITDKKEIEGLPPS 199

Query: 238 FF-----------ESLDKTENGKFKVILRSHHIAAVLEHCKVGTTRRMVAMAYGKRC--G 297
                              + G + + L +     V++H K    R  V  AY  R   G
Sbjct: 200 ALGLFAQAAVSKGHETATADTGPWLITLDAPSYLPVMQHAKNRALREEVYRAYLSRASSG 259

Query: 298 EV-NLSILENLVLLRHKFARLQGYSNYADYAVHYRMARSSSKVFEFLENISDSLTDLAAK 357
           ++ N +I++ ++ LR + A+L GY NYA+ ++  +MA +  K  E LE +  +  D A +
Sbjct: 260 DLDNTAIIDQILKLRLEKAKLLGYRNYAEVSMATKMA-TVEKADELLEKLRSASWDPAVQ 319

Query: 358 ELASLKDLKKQEEGESPFGIE--DLLYYVKKVEDQELNLDFMTLKQYFPVSLVLSGIFKI 417
           ++  LK   K +       +   D+ ++ +++ + + +++   L+ YF +  V+  +F +
Sbjct: 320 DIEDLKSFAKNQGAAEADSLTHWDITFWSERLRESKYDINEEELRPYFSLPKVMDALFGL 379

Query: 418 MQDLFGLRFEEVIDAE----VWHYDVKLYSVFDLNSGELIGYFFLDLYTREGK------- 477
            + LFG+   +V+ A+    VW+ DV+ Y V D +SG    YF+ D Y+R  +       
Sbjct: 380 AKTLFGI---DVVPADGVAPVWNSDVRFYCVKD-SSGNPTAYFYFDPYSRPSEKRDGAWM 439

Query: 478 ---YIHTCVVALQNSALLYNGTRQIPVALLISQLEKDVGGHAGLMRFTEVVNLFHEFGHV 537
              +  + V+A + S++      ++PVA ++      VG    LM F EV  +FHEFGH 
Sbjct: 440 DEVFSRSRVMAQKGSSV------RLPVAQMVCNQTPPVGDKPSLMTFREVETVFHEFGHA 499

Query: 538 VQHICNRASFTRISGLR-VDPDFVEIPAQILENWCYESVSLKLLSGFHQDITVPIKDEVC 597
           +QH+  +     ++G+R ++ D VE+P+Q +ENWCY   +L  ++  H      + + V 
Sbjct: 500 LQHMLTKEDEGLVAGIRNIEWDAVELPSQFMENWCYHRDTLMSIAK-HYQTGETLPENVY 559

Query: 598 ESLKKWRHSFSALKLKQEVLYCLFDQIIHCAENVDIVELFKHLHSKVMLGLPMLEGTNPA 657
           + L   R   +     +++ +   D  +H        E    +  +V +   ++    P 
Sbjct: 560 KKLLAARTFRAGSLSLRQLKFATVDLELHTKYMPGGAETIYEVDQRVSIKTQVIPPL-PE 619

Query: 658 SCFPCS-----AIGYEAACYSRVWSEVFSADIFVSKFRGDLLN-----QHTGLQFRNKVL 693
             F CS     A GY A  YS  W+EV SAD F S F    L+     + TG +FRN +L
Sbjct: 620 DRFLCSFSHIFAGGYAAGYYSYKWAEVLSADAF-SAFEDAGLDDIKAVKETGQRFRNTIL 679

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
XP_022996581.10.0e+0094.47probable thimet oligopeptidase [Cucurbita maxima][more]
KAG6598263.10.0e+0094.47putative thimet oligopeptidase, partial [Cucurbita argyrosperma subsp. sororia][more]
XP_022962337.10.0e+0094.04probable thimet oligopeptidase [Cucurbita moschata][more]
XP_023546722.10.0e+0093.90probable thimet oligopeptidase [Cucurbita pepo subsp. pepo][more]
XP_004142949.10.0e+0092.91probable thimet oligopeptidase isoform X1 [Cucumis sativus] >KGN62354.1 hypothet... [more]
Match NameE-valueIdentityDescription
F4HTQ13.0e-25360.00Probable thimet oligopeptidase OS=Arabidopsis thaliana OX=3702 GN=At1g67690 PE=3... [more]
P426753.0e-12037.33Neurolysin, mitochondrial OS=Oryctolagus cuniculus OX=9986 GN=NLN PE=1 SV=1[more]
Q91YP25.1e-12037.63Neurolysin, mitochondrial OS=Mus musculus OX=10090 GN=Nln PE=1 SV=1[more]
P426766.7e-12037.48Neurolysin, mitochondrial OS=Rattus norvegicus OX=10116 GN=Nln PE=1 SV=1[more]
Q020381.1e-11937.02Neurolysin, mitochondrial OS=Sus scrofa OX=9823 GN=NLN PE=1 SV=1[more]
Match NameE-valueIdentityDescription
A0A6J1K5680.0e+0094.47probable thimet oligopeptidase OS=Cucurbita maxima OX=3661 GN=LOC111491781 PE=3 ... [more]
A0A6J1HCG50.0e+0094.04probable thimet oligopeptidase OS=Cucurbita moschata OX=3662 GN=LOC111462816 PE=... [more]
A0A0A0LMX10.0e+0092.91Peptidase_M3 domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_2G35037... [more]
A0A1S3BAC20.0e+0093.19probable thimet oligopeptidase isoform X1 OS=Cucumis melo OX=3656 GN=LOC10348774... [more]
A0A6J1BRD70.0e+0093.05probable thimet oligopeptidase isoform X1 OS=Momordica charantia OX=3673 GN=LOC1... [more]
Match NameE-valueIdentityDescription
AT1G67690.12.1e-25460.00Zincin-like metalloproteases family protein [more]
AT5G65620.18.6e-5428.08Zincin-like metalloproteases family protein [more]
AT5G51540.11.1e-5325.59Zincin-like metalloproteases family protein [more]
AT5G10540.18.9e-5126.09Zincin-like metalloproteases family protein [more]
InterPro
Analysis Name: InterPro Annotations of Sponge gourd (AG-4) v1
Date Performed: 2022-08-01
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR024079Metallopeptidase, catalytic domain superfamilyGENE3D3.40.390.10Collagenase (Catalytic Domain)coord: 385..535
e-value: 1.6E-158
score: 530.4
IPR024080Neurolysin/Thimet oligopeptidase, N-terminalGENE3D1.20.1050.40Endopeptidase. Chain P; domain 1coord: 35..181
e-value: 4.7E-36
score: 125.6
IPR001567Peptidase M3A/M3B catalytic domainPFAMPF01432Peptidase_M3coord: 258..700
e-value: 1.2E-97
score: 328.1
IPR024077Neurolysin/Thimet oligopeptidase, domain 2GENE3D1.10.1370.10Neurolysin, domain 3coord: 191..697
e-value: 1.6E-158
score: 530.4
IPR045090Peptidase M3A/M3BPANTHERPTHR11804PROTEASE M3 THIMET OLIGOPEPTIDASE-RELATEDcoord: 44..702
NoneNo IPR availablePANTHERPTHR11804:SF40SACCHAROLYSINcoord: 44..702
NoneNo IPR availableCDDcd06455M3A_TOPcoord: 68..698
e-value: 0.0
score: 723.147
NoneNo IPR availableSUPERFAMILY55486Metalloproteases ("zincins"), catalytic domaincoord: 58..698

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Lag0004695.1Lag0004695.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0006518 peptide metabolic process
biological_process GO:0006508 proteolysis
cellular_component GO:0016021 integral component of membrane
molecular_function GO:0046872 metal ion binding
molecular_function GO:0004222 metalloendopeptidase activity
molecular_function GO:0008237 metallopeptidase activity