Lag0004688 (gene) Sponge gourd (AG‐4) v1

Overview
NameLag0004688
Typegene
OrganismLuffa acutangula (Sponge gourd (AG‐4) v1)
Descriptionvinorine synthase-like
Locationchr6: 6102062 .. 6102892 (+)
RNA-Seq ExpressionLag0004688
SyntenyLag0004688
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonCDSpolypeptide
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGAAGCTTGAAGTTGACATAATTTCTGAAGAGCTCATCAAACCTTCTACTCCAACTCCTCCCCATCTCCGCCATTACCCGCTCTCATTCATCGATCAGATCACCGTTCACATTTACGCTCCCGCCCTCTACTTCTACCGGTCCAACGACCCCGAAGATCTCGATTTCGCTCTCGCCTTCAAAAACCGCCTCCGTAGCTCCCTCCCCGTCGTCCTCTCCCGCTACCCTCCCCTCGCGGGCCGCCCCAACTACGCCTCCTCCTTCATCGACTGCAACGACACCGGCGTCCCATTCCTCGAAGCCCGGGTGAACTCGCGACTCGCCGACGTGGTCCACTTGGCCCGGCCGGACGACCTGAACCGGCTGTTCCCGGTCGAGCTGGACCGGCCCAACGAGGAGTTGATGGCGGTTCAGTTCACAGAATTCGCGTGCGGCGGAGTGGCGTTGGCGTCGTGCATTTCGCACAAGATCGCCGACGCCATGACGCTGTTCTCGCTGAACAACAACTGGGCGGCGGTGGCTCGTGGGGCTAAGGGCGGCGTTTGGAAGCCGCACATGGAAGGGGGTAAGTTGTTTCCGGCGAAAACGATGTCGTATGACACGTCGATGACGATCGTGAGGAATAGAGTGGCGAGGAGGTTCGTGTTTGAGGGTTCGAAAGTGGAAGCCATTAGAGGGAAATATGCAGAGAGTGGAGCCATGGGAAACCAGACTCGACCGTCGCGGGTTGAGGCTTTGTCGGCGTTTGTTTACAGCCGGTTTCTCGCCGCCGTTAAGGATGGTTCTTCTCAACTTCCGACCGACAGGTCTCATCGAAACCCACGAACTTAA

mRNA sequence

ATGAAGCTTGAAGTTGACATAATTTCTGAAGAGCTCATCAAACCTTCTACTCCAACTCCTCCCCATCTCCGCCATTACCCGCTCTCATTCATCGATCAGATCACCGTTCACATTTACGCTCCCGCCCTCTACTTCTACCGGTCCAACGACCCCGAAGATCTCGATTTCGCTCTCGCCTTCAAAAACCGCCTCCGTAGCTCCCTCCCCGTCGTCCTCTCCCGCTACCCTCCCCTCGCGGGCCGCCCCAACTACGCCTCCTCCTTCATCGACTGCAACGACACCGGCGTCCCATTCCTCGAAGCCCGGGTGAACTCGCGACTCGCCGACGTGGTCCACTTGGCCCGGCCGGACGACCTGAACCGGCTGTTCCCGGTCGAGCTGGACCGGCCCAACGAGGAGTTGATGGCGGTTCAGTTCACAGAATTCGCGTGCGGCGGAGTGGCGTTGGCGTCGTGCATTTCGCACAAGATCGCCGACGCCATGACGCTGTTCTCGCTGAACAACAACTGGGCGGCGGTGGCTCGTGGGGCTAAGGGCGGCGTTTGGAAGCCGCACATGGAAGGGGGTAAGTTGTTTCCGGCGAAAACGATGTCGTATGACACGTCGATGACGATCGTGAGGAATAGAGTGGCGAGGAGGTTCGTGTTTGAGGGTTCGAAAGTGGAAGCCATTAGAGGGAAATATGCAGAGAGTGGAGCCATGGGAAACCAGACTCGACCGTCGCGGGTTGAGGCTTTGTCGGCGTTTGTTTACAGCCGGTTTCTCGCCGCCGTTAAGGATGGTTCTTCTCAACTTCCGACCGACAGGTCTCATCGAAACCCACGAACTTAA

Coding sequence (CDS)

ATGAAGCTTGAAGTTGACATAATTTCTGAAGAGCTCATCAAACCTTCTACTCCAACTCCTCCCCATCTCCGCCATTACCCGCTCTCATTCATCGATCAGATCACCGTTCACATTTACGCTCCCGCCCTCTACTTCTACCGGTCCAACGACCCCGAAGATCTCGATTTCGCTCTCGCCTTCAAAAACCGCCTCCGTAGCTCCCTCCCCGTCGTCCTCTCCCGCTACCCTCCCCTCGCGGGCCGCCCCAACTACGCCTCCTCCTTCATCGACTGCAACGACACCGGCGTCCCATTCCTCGAAGCCCGGGTGAACTCGCGACTCGCCGACGTGGTCCACTTGGCCCGGCCGGACGACCTGAACCGGCTGTTCCCGGTCGAGCTGGACCGGCCCAACGAGGAGTTGATGGCGGTTCAGTTCACAGAATTCGCGTGCGGCGGAGTGGCGTTGGCGTCGTGCATTTCGCACAAGATCGCCGACGCCATGACGCTGTTCTCGCTGAACAACAACTGGGCGGCGGTGGCTCGTGGGGCTAAGGGCGGCGTTTGGAAGCCGCACATGGAAGGGGGTAAGTTGTTTCCGGCGAAAACGATGTCGTATGACACGTCGATGACGATCGTGAGGAATAGAGTGGCGAGGAGGTTCGTGTTTGAGGGTTCGAAAGTGGAAGCCATTAGAGGGAAATATGCAGAGAGTGGAGCCATGGGAAACCAGACTCGACCGTCGCGGGTTGAGGCTTTGTCGGCGTTTGTTTACAGCCGGTTTCTCGCCGCCGTTAAGGATGGTTCTTCTCAACTTCCGACCGACAGGTCTCATCGAAACCCACGAACTTAA

Protein sequence

MKLEVDIISEELIKPSTPTPPHLRHYPLSFIDQITVHIYAPALYFYRSNDPEDLDFALAFKNRLRSSLPVVLSRYPPLAGRPNYASSFIDCNDTGVPFLEARVNSRLADVVHLARPDDLNRLFPVELDRPNEELMAVQFTEFACGGVALASCISHKIADAMTLFSLNNNWAAVARGAKGGVWKPHMEGGKLFPAKTMSYDTSMTIVRNRVARRFVFEGSKVEAIRGKYAESGAMGNQTRPSRVEALSAFVYSRFLAAVKDGSSQLPTDRSHRNPRT
Homology
BLAST of Lag0004688 vs. NCBI nr
Match: XP_038886173.1 (stemmadenine O-acetyltransferase-like [Benincasa hispida])

HSP 1 Score: 437.6 bits (1124), Expect = 8.1e-119
Identity = 222/267 (83.15%), Postives = 239/267 (89.51%), Query Frame = 0

Query: 1   MKLEVDIISEELIKPSTPTPPHLRHYPLSFIDQITVHIYAPALYFYRSNDP----EDLDF 60
           MKLEVDIISEELIKPSTPTPPHLR YPLS IDQITVHIYAP LYFYRS D     EDLDF
Sbjct: 1   MKLEVDIISEELIKPSTPTPPHLRRYPLSLIDQITVHIYAPTLYFYRSTDSGSGGEDLDF 60

Query: 61  ALAFKNRLRSSLPVVLSRYPPLAGRPNYASSFIDCNDTGVPFLEARVNSRLADVVHLARP 120
           ALAFKNRLRS+LP VLS YP LAGRPNYASSFI+CND GVPF EARVNS+LADVV  A+P
Sbjct: 61  ALAFKNRLRSTLPDVLSHYPQLAGRPNYASSFINCNDAGVPFREARVNSQLADVVQFAQP 120

Query: 121 DDLNRLFPVELDRPNEELMAVQFTEFACGGVALASCISHKIADAMTLFSLNNNWAAVARG 180
           DDLNRLFPVELDR NEELMAVQFTEFACGGVA+ASCISHKIADAMTLFSLNNNWAA+ARG
Sbjct: 121 DDLNRLFPVELDRFNEELMAVQFTEFACGGVAVASCISHKIADAMTLFSLNNNWAAMARG 180

Query: 181 AKGGVWKPHMEGGKLFPAKTMSYDTSMTIVRNRVARRFVFEGSKVEAIRGKYAESGAMGN 240
            K  V+KPHMEG K+FPAKTMSYDT+MT+VRNRVARRFVFE SK+EAIR KYAE+ A  N
Sbjct: 181 VK-DVFKPHMEGAKIFPAKTMSYDTAMTLVRNRVARRFVFEQSKLEAIRAKYAENQATIN 240

Query: 241 QTRPSRVEALSAFVYSRFLAAVKDGSS 264
           Q RPSR+E+LSAFVYSRF+AA+K GS+
Sbjct: 241 QIRPSRIESLSAFVYSRFIAALKHGSA 266

BLAST of Lag0004688 vs. NCBI nr
Match: XP_008445555.1 (PREDICTED: vinorine synthase-like [Cucumis melo])

HSP 1 Score: 434.5 bits (1116), Expect = 6.9e-118
Identity = 219/267 (82.02%), Postives = 237/267 (88.76%), Query Frame = 0

Query: 1   MKLEVDIISEELIKPSTPTPPHLRHYPLSFIDQITVHIYAPALYFYRSNDP----EDLDF 60
           MKLEVDIISEELIKPSTPTPPHLRHY LSFIDQITVHIYAPALYFY+S D     EDLDF
Sbjct: 1   MKLEVDIISEELIKPSTPTPPHLRHYSLSFIDQITVHIYAPALYFYQSTDTGVGGEDLDF 60

Query: 61  ALAFKNRLRSSLPVVLSRYPPLAGRPNYASSFIDCNDTGVPFLEARVNSRLADVVHLARP 120
           ALAFKNRLRS+LP VLS YPPLAGRPNYASSFIDCNDTGVPF EARVNS+LADV+  A+P
Sbjct: 61  ALAFKNRLRSTLPDVLSHYPPLAGRPNYASSFIDCNDTGVPFREARVNSQLADVIQFAQP 120

Query: 121 DDLNRLFPVELDRPNEELMAVQFTEFACGGVALASCISHKIADAMTLFSLNNNWAAVARG 180
           DDLNRLFPVELDR NEELMAVQFTEFACGGVA+ASCISHKIADAM LFSLNNNWAA+ARG
Sbjct: 121 DDLNRLFPVELDRFNEELMAVQFTEFACGGVAVASCISHKIADAMALFSLNNNWAAMARG 180

Query: 181 AKGGVWKPHMEGGKLFPAKTMSYDTSMTIVRNRVARRFVFEGSKVEAIRGKYAESGAMGN 240
            K GV+KPHMEG K+FP K MSYDT+MTIVRNRV+RRFVF+ SK+EAIR KY E+    N
Sbjct: 181 VK-GVFKPHMEGAKIFPPKMMSYDTAMTIVRNRVSRRFVFKQSKLEAIRAKYTENQTTIN 240

Query: 241 QTRPSRVEALSAFVYSRFLAAVKDGSS 264
           Q RPSR+E+L+AFVYSRFLAA K  S+
Sbjct: 241 QNRPSRIESLTAFVYSRFLAAFKHDSA 266

BLAST of Lag0004688 vs. NCBI nr
Match: KAA0061032.1 (vinorine synthase-like [Cucumis melo var. makuwa])

HSP 1 Score: 434.5 bits (1116), Expect = 6.9e-118
Identity = 219/267 (82.02%), Postives = 237/267 (88.76%), Query Frame = 0

Query: 1   MKLEVDIISEELIKPSTPTPPHLRHYPLSFIDQITVHIYAPALYFYRSNDP----EDLDF 60
           MKLEVDIISEELIKPSTPTPPHLRHY LSFIDQITVHIYAPALYFY+S D     EDLDF
Sbjct: 1   MKLEVDIISEELIKPSTPTPPHLRHYSLSFIDQITVHIYAPALYFYQSTDTGVGGEDLDF 60

Query: 61  ALAFKNRLRSSLPVVLSRYPPLAGRPNYASSFIDCNDTGVPFLEARVNSRLADVVHLARP 120
           ALAFKNRLRS+LP VLS YPPLAGRPNYASSFIDCNDTGVPF EARVNS+LADV+  A+P
Sbjct: 61  ALAFKNRLRSTLPDVLSHYPPLAGRPNYASSFIDCNDTGVPFREARVNSQLADVIQFAQP 120

Query: 121 DDLNRLFPVELDRPNEELMAVQFTEFACGGVALASCISHKIADAMTLFSLNNNWAAVARG 180
           DDLNRLFPVELDR NEELMAVQFTEFACGGVA+ASCISHKIADAM LFSLNNNWAA+ARG
Sbjct: 121 DDLNRLFPVELDRFNEELMAVQFTEFACGGVAVASCISHKIADAMALFSLNNNWAAMARG 180

Query: 181 AKGGVWKPHMEGGKLFPAKTMSYDTSMTIVRNRVARRFVFEGSKVEAIRGKYAESGAMGN 240
            K GV+KPHMEG K+FP K MSYDT+MTIVRNRV+RRFVF+ SK+EAIR KY E+    N
Sbjct: 181 VK-GVFKPHMEGAKIFPPKMMSYDTAMTIVRNRVSRRFVFKQSKLEAIRAKYTENQTTIN 240

Query: 241 QTRPSRVEALSAFVYSRFLAAVKDGSS 264
           Q RPSR+E+L+AFVYSRFLAA K  S+
Sbjct: 241 QNRPSRIESLTAFVYSRFLAAFKHDSA 266

BLAST of Lag0004688 vs. NCBI nr
Match: XP_022951641.1 (vinorine synthase-like [Cucurbita moschata])

HSP 1 Score: 429.5 bits (1103), Expect = 2.2e-116
Identity = 216/271 (79.70%), Postives = 242/271 (89.30%), Query Frame = 0

Query: 1   MKLEVDIISEELIKPSTPTPPHLRHYPLSFIDQITVHIYAPALYFYRSNDPEDLDFALAF 60
           MKLE+DIISE+LIKP+TPTPPHLR YPLSFIDQITVHIYAPALYFY+ N   D DFA+ F
Sbjct: 1   MKLEIDIISEDLIKPATPTPPHLRRYPLSFIDQITVHIYAPALYFYQPNRAGDPDFAIDF 60

Query: 61  KNRLRSSLPVVLSRYPPLAGRPNYASSFIDCNDTGVPFLEARVNSRLADVVHLARPDDLN 120
           +NRLRS+L VVLS YPPLAGRPNY S+F+DCNDTG  F EARVN++LADVV  ARPDDLN
Sbjct: 61  RNRLRSTLSVVLSHYPPLAGRPNYDSAFVDCNDTGALFREARVNTKLADVVEYARPDDLN 120

Query: 121 RLFPVELDRPNEELMAVQFTEFACGGVALASCISHKIADAMTLFSLNNNWAAVARGAKGG 180
           RLFPVELD+ NEELMAVQFTEFACGGVA+ASCISHKIADAMTLFSLNNNWAA+ARG K G
Sbjct: 121 RLFPVELDQFNEELMAVQFTEFACGGVAVASCISHKIADAMTLFSLNNNWAAMARGVK-G 180

Query: 181 VWKPHMEGGKLFPAKTMSYDTSMTIVRNRVARRFVFEGSKVEAIRGKYAESGAMGNQTRP 240
           V+KPHMEG KLFPAKTM+YDT+ TI+RNRVARRFVFEGSKVEAIR KY E+GA     RP
Sbjct: 181 VFKPHMEGAKLFPAKTMTYDTAKTILRNRVARRFVFEGSKVEAIREKYGENGA---AHRP 240

Query: 241 SRVEALSAFVYSRFLAAVKDG-SSQLPTDRS 271
           SRVE+L+AF+Y RFLAA+KDG SSQ+P+DR+
Sbjct: 241 SRVESLTAFMYGRFLAALKDGSSSQVPSDRT 267

BLAST of Lag0004688 vs. NCBI nr
Match: XP_004143048.1 (stemmadenine O-acetyltransferase [Cucumis sativus] >KGN62351.1 hypothetical protein Csa_018752 [Cucumis sativus])

HSP 1 Score: 428.7 bits (1101), Expect = 3.8e-116
Identity = 219/267 (82.02%), Postives = 237/267 (88.76%), Query Frame = 0

Query: 1   MKLEVDIISEELIKPSTPTPPHLRHYPLSFIDQITVHIYAPALYFYRSNDP----EDLDF 60
           MKLEVDIISEELIKPSTPTPPHLRHY LSFIDQITVHIYAPALYFY+S D     EDLDF
Sbjct: 1   MKLEVDIISEELIKPSTPTPPHLRHYSLSFIDQITVHIYAPALYFYQSTDTGVGGEDLDF 60

Query: 61  ALAFKNRLRSSLPVVLSRYPPLAGRPNYASSFIDCNDTGVPFLEARVNSRLADVVHLARP 120
           ALAFKNRLRS+LP VLS YPPLAGRPNYASSFIDCNDTGVPF EA VNS+LADV+  A+P
Sbjct: 61  ALAFKNRLRSTLPDVLSHYPPLAGRPNYASSFIDCNDTGVPFREALVNSQLADVIQFAQP 120

Query: 121 DDLNRLFPVELDRPNEELMAVQFTEFACGGVALASCISHKIADAMTLFSLNNNWAAVARG 180
           DDLNRLFPVELDR NEELMAVQFTEFACGGVA+ASCISHKIADAMTLFSLNNNWAA+ARG
Sbjct: 121 DDLNRLFPVELDRFNEELMAVQFTEFACGGVAVASCISHKIADAMTLFSLNNNWAAMARG 180

Query: 181 AKGGVWKPHMEGGKLFPAKTMSYDTSMTIVRNRVARRFVFEGSKVEAIRGKYAESGAMGN 240
            K GV+KPHMEG K+FP K MSYD++MTIVRNRV+RRFVF+ SKVEAIR KY E+  M N
Sbjct: 181 VK-GVFKPHMEGAKIFPPKPMSYDSAMTIVRNRVSRRFVFKQSKVEAIRTKYTENQTMIN 240

Query: 241 QT-RPSRVEALSAFVYSRFLAAVKDGS 263
           Q  +PSR+E+L+AFVYSRFLAA K  S
Sbjct: 241 QNHQPSRIESLTAFVYSRFLAAFKHDS 266

BLAST of Lag0004688 vs. ExPASy Swiss-Prot
Match: A0A2P1GIW7 (Stemmadenine O-acetyltransferase OS=Catharanthus roseus OX=4058 GN=SAT PE=1 SV=1)

HSP 1 Score: 160.6 bits (405), Expect = 2.5e-38
Identity = 98/263 (37.26%), Postives = 153/263 (58.17%), Query Frame = 0

Query: 1   MKLEVDIISEELIKPSTPTPPHLRHYPLSFIDQITVHIYAPALYFYRSNDPEDLDFALAF 60
           M  ++ I+SEELI+PS+PTP  L+ + LS +DQ+ +  + P + FY +    +LD A   
Sbjct: 1   MAPQMQILSEELIQPSSPTPQTLKTHKLSHLDQVLLTCHIPIILFYPNQLDSNLDRAQRS 60

Query: 61  KNRLRSSLPVVLSRYPPLAGRPNYASSFIDCNDTGVPFLEARVNSRLADVVHLARPDDLN 120
           +N L+ SL  VL+++ PLAGR N  SS +DCND+GVPFLEARV+S+L++ +     D+LN
Sbjct: 61  EN-LKRSLSTVLTQFYPLAGRININSS-VDCNDSGVPFLEARVHSQLSEAIKNVAIDELN 120

Query: 121 RLFPVELDRPNEEL-------MAVQFTEFACGGVALASCISHKIADAMTLFSLNNNWAAV 180
           +  P +     EE        +AV+ + F CGG A+  CISHKIADA++L +  N+W A 
Sbjct: 121 QYLPFQPYPGGEESGLKKDIPLAVKISCFECGGTAIGVCISHKIADALSLATFLNSWTAT 180

Query: 181 ARGAKGGVWKPHME-GGKLFPAKTMSYDTSMTIVRNRVARRFVFEGSKVEAIRGKYAESG 240
            +  +  + +P+ + G   FP              N V +RFVF+  K+EA++ + A S 
Sbjct: 181 CQ-EETDIVQPNFDLGSHHFPPMESIPAPEFLPDENIVMKRFVFDKEKLEALKAQLASSA 240

Query: 241 AMGNQTRPSRVEALSAFVYSRFL 256
                   SRV+ + A ++ +F+
Sbjct: 241 T--EVKNSSRVQIVIAVIWKQFI 258

BLAST of Lag0004688 vs. ExPASy Swiss-Prot
Match: Q70PR7 (Vinorine synthase OS=Rauvolfia serpentina OX=4060 GN=ACT PE=1 SV=2)

HSP 1 Score: 140.6 bits (353), Expect = 2.7e-32
Identity = 91/262 (34.73%), Postives = 145/262 (55.34%), Query Frame = 0

Query: 1   MKLEVDIISEELIKPSTPTPPHLRHYPLSFIDQITVHIYAPALYFYRSNDPEDLDFALAF 60
           M  +++ +SEELI PS+PTP  L+ Y +S +DQ+ +  + P + FY +    +LD A   
Sbjct: 1   MAPQMEKVSEELILPSSPTPQSLKCYKISHLDQLLLTCHIPFILFYPNPLDSNLDPAQT- 60

Query: 61  KNRLRSSLPVVLSRYPPLAGRPNYASSFIDCNDTGVPFLEARVNSRLA----DVVHLARP 120
              L+ SL  VL+ + PLAGR N  SS +DCND+GVPF+EARV ++L+    +VV L + 
Sbjct: 61  SQHLKQSLSKVLTHFYPLAGRINVNSS-VDCNDSGVPFVEARVQAQLSQAIQNVVELEKL 120

Query: 121 DDL--NRLFPVELDRPNEEL-MAVQFTEFACGGVALASCISHKIADAMTLFSLNNNWAAV 180
           D    +  +P      NE++ +AV+ + F CGG A+   +SHKIAD ++L +  N W A 
Sbjct: 121 DQYLPSAAYPGGKIEVNEDVPLAVKISFFECGGTAIGVNLSHKIADVLSLATFLNAWTAT 180

Query: 181 ARGAKGGVWKPHMEGGKLFPAKTMSYDTSMTIVRNRVARRFVFEGSKVEAIRGKYAESGA 240
            RG    V        + FP    +    +    N V +RFVF+  K+ A+R   A++ +
Sbjct: 181 CRGETEIVLPNFDLAARHFPPVDNTPSPELVPDENVVMKRFVFDKEKIGALR---AQASS 240

Query: 241 MGNQTRPSRVEALSAFVYSRFL 256
              +   SRV+ + A+++   +
Sbjct: 241 ASEEKNFSRVQLVVAYIWKHVI 257

BLAST of Lag0004688 vs. ExPASy Swiss-Prot
Match: A0A2G3D6U1 (Acyltransferase Pun1 OS=Capsicum chinense OX=80379 GN=PUN1 PE=2 SV=1)

HSP 1 Score: 136.0 bits (341), Expect = 6.6e-31
Identity = 92/284 (32.39%), Postives = 144/284 (50.70%), Query Frame = 0

Query: 5   VDIISEELIKPSTPTPPHLRHYPLSFIDQITVHIYAPALYFY-----RSNDPEDLDFALA 64
           V +  +  IKPS+ TP  LR + LSFIDQ   ++Y P  +FY     R  D ++ D    
Sbjct: 10  VSVCDKSFIKPSSLTPSTLRFHKLSFIDQSLSNMYIPCAFFYPKVQQRLEDSKNSDELSH 69

Query: 65  FKNRLRSSLPVVLSRYPPLAGRPNYASSFIDCNDTGVPFLEARVNSRLADVV---HLARP 124
             + L++SL   L  Y P AG+    ++ +DCND G  FL  R+   +++++   H +  
Sbjct: 70  IAHLLQTSLSQTLVSYYPYAGKLK-DNATVDCNDMGAEFLSVRIKCSMSEILDHPHASLA 129

Query: 125 DDLNRLFPVELDRPNE----ELMAVQFTEFACGGVALASCISHKIADAMTLFSLNNNWAA 184
           + +  + P +L   N      L+ VQ ++F CGG+A++ C SHKI D  +L +  N+W++
Sbjct: 130 ESI--VLPKDLPWANNCEGGNLLVVQVSKFDCGGIAISVCFSHKIGDGCSLLNFLNDWSS 189

Query: 185 VARG--AKGGVWKPHMEGGKLFPAKTMSYDTSMTIVRNR---VARRFVFEGSKVEAIRGK 244
           V R       V  P   G  +F  KT    T+  I+ +    V +R +F   K++A+R K
Sbjct: 190 VTRDHTTTTLVPSPRFVGDSVFSTKTYGSLTTPQILSDLNECVQKRLIFPTDKLDALRAK 249

Query: 245 YAESGAMGNQTRPSRVEALSAFVYSRFLAAVKDGSSQLPTDRSH 272
            AE   + N   P+R E +SA +   F  A K  SS LP+   H
Sbjct: 250 VAEESGVKN---PTRAEVVSALL---FKCATKASSSMLPSKLVH 284

BLAST of Lag0004688 vs. ExPASy Swiss-Prot
Match: Q8GZU0 (Minovincinine 19-hydroxy-O-acetyltransferase OS=Catharanthus roseus OX=4058 GN=MAT PE=1 SV=1)

HSP 1 Score: 132.9 bits (333), Expect = 5.6e-30
Identity = 87/257 (33.85%), Postives = 134/257 (52.14%), Query Frame = 0

Query: 3   LEVDIISEELIKPSTPTPPHLRHYPLSFIDQITVHIYAPALYFYRSNDPEDLDFALAFKN 62
           +E + +S+ LIKPS+PTP  L HY LS+ DQ     Y  A +FY + D  ++      + 
Sbjct: 7   VETETLSKTLIKPSSPTPQSLSHYNLSYNDQNIYPEYIFAGFFYSNPDGHEIS---TIRE 66

Query: 63  RLRSSLPVVLSRYPPLAGRPNYASSFIDCNDTGVPFLEARVNSRLADVVHLARPDDLNRL 122
           +L++SL   L  Y P AG+    + +I CND G+ F++ R++ R+ D++   +P+   R 
Sbjct: 67  QLQNSLSKTLVSYYPFAGKV-VKNDYIHCNDDGIEFVDVRIHCRMNDIL---KPE--LRS 126

Query: 123 FPVELDRPNEELMA-------VQFTEFACGGVALASCISHKIADAMTLFSLNNNWAAVA- 182
           +  EL RPN   +        VQ + F CGGVA+A  ISHK+ADA T+ S   +WAA   
Sbjct: 127 YASELIRPNRSTVGSEDSTALVQLSHFDCGGVAVAFGISHKVADAATILSFIKDWAASTC 186

Query: 183 -RGAKGGVWKPHMEGGKLFPAKTMSYDTSMTIVRNRVARRFVFEGSKVEAIRGKYAESGA 242
              +   V  P +    +FP +            N V +RF+F    +E ++ K  E G 
Sbjct: 187 DLSSSHDVSTPVLVSDSIFPRQDNIICGQFPASPNCVRKRFLFSPEAIERLKSKAIEFGI 246

Query: 243 MGNQTRPSRVEALSAFV 251
                +P+RVE L+AF+
Sbjct: 247 ----EKPTRVEVLTAFL 250

BLAST of Lag0004688 vs. ExPASy Swiss-Prot
Match: D2Y3X2 (Acyltransferase Pun1 OS=Capsicum annuum OX=4072 GN=PUN1 PE=1 SV=1)

HSP 1 Score: 132.1 bits (331), Expect = 9.6e-30
Identity = 91/286 (31.82%), Postives = 144/286 (50.35%), Query Frame = 0

Query: 5   VDIISEELIKPSTPTPPHLRHYPLSFIDQITVHIYAPALYFY-----RSNDPEDLDFALA 64
           V + ++  IKPS+ TP  LR + LSFIDQ   ++Y P  +FY     R  D ++ D    
Sbjct: 10  VSVCNKSFIKPSSLTPSTLRFHKLSFIDQSLSNMYIPCAFFYPKVQQRLEDSKNSDELSH 69

Query: 65  FKNRLRSSLPVVLSRYPPLAGRPNYASSFIDCNDTGVPFLEARVNSRLADVV---HLARP 124
             + L++SL   L  Y P AG+    ++ +DCND G  FL  R+   +++++   H +  
Sbjct: 70  IAHLLQTSLSQTLVSYYPYAGKLK-DNATVDCNDMGAEFLSVRIKCSMSEILDHPHASLA 129

Query: 125 DDLNRLFPVELDRPNE----ELMAVQFTEFACGGVALASCISHKIADAMTLFSLNNNWAA 184
           + +  + P +L   N      L+ VQ ++F CGG+A++ C SHKI D  +L +  N+W++
Sbjct: 130 ESI--VLPKDLPWANNCEGGNLLVVQVSKFDCGGIAISVCFSHKIGDGCSLLNFLNDWSS 189

Query: 185 VARGAKGG--VWKPHMEGGKLFPAKTMSYDTSMT-----IVRNRVARRFVFEGSKVEAIR 244
           V R       V  P   G  +F   T  Y + +T      +   V +R +F   K++A+R
Sbjct: 190 VTRDRTTTTLVPSPRFVGDSVF--STQKYGSLITPQILSDLNQCVQKRLIFPTDKLDALR 249

Query: 245 GKYAESGAMGNQTRPSRVEALSAFVYSRFLAAVKDGSSQLPTDRSH 272
            K AE   + N   P+R E +SA +   F  A K  SS LP+   H
Sbjct: 250 AKVAEESGVKN---PTRAEVVSALL---FKCATKASSSMLPSKLVH 284

BLAST of Lag0004688 vs. ExPASy TrEMBL
Match: A0A5A7UYQ7 (Vinorine synthase-like OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold501G001680 PE=3 SV=1)

HSP 1 Score: 434.5 bits (1116), Expect = 3.3e-118
Identity = 219/267 (82.02%), Postives = 237/267 (88.76%), Query Frame = 0

Query: 1   MKLEVDIISEELIKPSTPTPPHLRHYPLSFIDQITVHIYAPALYFYRSNDP----EDLDF 60
           MKLEVDIISEELIKPSTPTPPHLRHY LSFIDQITVHIYAPALYFY+S D     EDLDF
Sbjct: 1   MKLEVDIISEELIKPSTPTPPHLRHYSLSFIDQITVHIYAPALYFYQSTDTGVGGEDLDF 60

Query: 61  ALAFKNRLRSSLPVVLSRYPPLAGRPNYASSFIDCNDTGVPFLEARVNSRLADVVHLARP 120
           ALAFKNRLRS+LP VLS YPPLAGRPNYASSFIDCNDTGVPF EARVNS+LADV+  A+P
Sbjct: 61  ALAFKNRLRSTLPDVLSHYPPLAGRPNYASSFIDCNDTGVPFREARVNSQLADVIQFAQP 120

Query: 121 DDLNRLFPVELDRPNEELMAVQFTEFACGGVALASCISHKIADAMTLFSLNNNWAAVARG 180
           DDLNRLFPVELDR NEELMAVQFTEFACGGVA+ASCISHKIADAM LFSLNNNWAA+ARG
Sbjct: 121 DDLNRLFPVELDRFNEELMAVQFTEFACGGVAVASCISHKIADAMALFSLNNNWAAMARG 180

Query: 181 AKGGVWKPHMEGGKLFPAKTMSYDTSMTIVRNRVARRFVFEGSKVEAIRGKYAESGAMGN 240
            K GV+KPHMEG K+FP K MSYDT+MTIVRNRV+RRFVF+ SK+EAIR KY E+    N
Sbjct: 181 VK-GVFKPHMEGAKIFPPKMMSYDTAMTIVRNRVSRRFVFKQSKLEAIRAKYTENQTTIN 240

Query: 241 QTRPSRVEALSAFVYSRFLAAVKDGSS 264
           Q RPSR+E+L+AFVYSRFLAA K  S+
Sbjct: 241 QNRPSRIESLTAFVYSRFLAAFKHDSA 266

BLAST of Lag0004688 vs. ExPASy TrEMBL
Match: A0A1S3BD01 (vinorine synthase-like OS=Cucumis melo OX=3656 GN=LOC103488534 PE=3 SV=1)

HSP 1 Score: 434.5 bits (1116), Expect = 3.3e-118
Identity = 219/267 (82.02%), Postives = 237/267 (88.76%), Query Frame = 0

Query: 1   MKLEVDIISEELIKPSTPTPPHLRHYPLSFIDQITVHIYAPALYFYRSNDP----EDLDF 60
           MKLEVDIISEELIKPSTPTPPHLRHY LSFIDQITVHIYAPALYFY+S D     EDLDF
Sbjct: 1   MKLEVDIISEELIKPSTPTPPHLRHYSLSFIDQITVHIYAPALYFYQSTDTGVGGEDLDF 60

Query: 61  ALAFKNRLRSSLPVVLSRYPPLAGRPNYASSFIDCNDTGVPFLEARVNSRLADVVHLARP 120
           ALAFKNRLRS+LP VLS YPPLAGRPNYASSFIDCNDTGVPF EARVNS+LADV+  A+P
Sbjct: 61  ALAFKNRLRSTLPDVLSHYPPLAGRPNYASSFIDCNDTGVPFREARVNSQLADVIQFAQP 120

Query: 121 DDLNRLFPVELDRPNEELMAVQFTEFACGGVALASCISHKIADAMTLFSLNNNWAAVARG 180
           DDLNRLFPVELDR NEELMAVQFTEFACGGVA+ASCISHKIADAM LFSLNNNWAA+ARG
Sbjct: 121 DDLNRLFPVELDRFNEELMAVQFTEFACGGVAVASCISHKIADAMALFSLNNNWAAMARG 180

Query: 181 AKGGVWKPHMEGGKLFPAKTMSYDTSMTIVRNRVARRFVFEGSKVEAIRGKYAESGAMGN 240
            K GV+KPHMEG K+FP K MSYDT+MTIVRNRV+RRFVF+ SK+EAIR KY E+    N
Sbjct: 181 VK-GVFKPHMEGAKIFPPKMMSYDTAMTIVRNRVSRRFVFKQSKLEAIRAKYTENQTTIN 240

Query: 241 QTRPSRVEALSAFVYSRFLAAVKDGSS 264
           Q RPSR+E+L+AFVYSRFLAA K  S+
Sbjct: 241 QNRPSRIESLTAFVYSRFLAAFKHDSA 266

BLAST of Lag0004688 vs. ExPASy TrEMBL
Match: A0A6J1GI32 (vinorine synthase-like OS=Cucurbita moschata OX=3662 GN=LOC111454395 PE=3 SV=1)

HSP 1 Score: 429.5 bits (1103), Expect = 1.1e-116
Identity = 216/271 (79.70%), Postives = 242/271 (89.30%), Query Frame = 0

Query: 1   MKLEVDIISEELIKPSTPTPPHLRHYPLSFIDQITVHIYAPALYFYRSNDPEDLDFALAF 60
           MKLE+DIISE+LIKP+TPTPPHLR YPLSFIDQITVHIYAPALYFY+ N   D DFA+ F
Sbjct: 1   MKLEIDIISEDLIKPATPTPPHLRRYPLSFIDQITVHIYAPALYFYQPNRAGDPDFAIDF 60

Query: 61  KNRLRSSLPVVLSRYPPLAGRPNYASSFIDCNDTGVPFLEARVNSRLADVVHLARPDDLN 120
           +NRLRS+L VVLS YPPLAGRPNY S+F+DCNDTG  F EARVN++LADVV  ARPDDLN
Sbjct: 61  RNRLRSTLSVVLSHYPPLAGRPNYDSAFVDCNDTGALFREARVNTKLADVVEYARPDDLN 120

Query: 121 RLFPVELDRPNEELMAVQFTEFACGGVALASCISHKIADAMTLFSLNNNWAAVARGAKGG 180
           RLFPVELD+ NEELMAVQFTEFACGGVA+ASCISHKIADAMTLFSLNNNWAA+ARG K G
Sbjct: 121 RLFPVELDQFNEELMAVQFTEFACGGVAVASCISHKIADAMTLFSLNNNWAAMARGVK-G 180

Query: 181 VWKPHMEGGKLFPAKTMSYDTSMTIVRNRVARRFVFEGSKVEAIRGKYAESGAMGNQTRP 240
           V+KPHMEG KLFPAKTM+YDT+ TI+RNRVARRFVFEGSKVEAIR KY E+GA     RP
Sbjct: 181 VFKPHMEGAKLFPAKTMTYDTAKTILRNRVARRFVFEGSKVEAIREKYGENGA---AHRP 240

Query: 241 SRVEALSAFVYSRFLAAVKDG-SSQLPTDRS 271
           SRVE+L+AF+Y RFLAA+KDG SSQ+P+DR+
Sbjct: 241 SRVESLTAFMYGRFLAALKDGSSSQVPSDRT 267

BLAST of Lag0004688 vs. ExPASy TrEMBL
Match: A0A0A0LQS8 (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_2G350340 PE=3 SV=1)

HSP 1 Score: 428.7 bits (1101), Expect = 1.8e-116
Identity = 219/267 (82.02%), Postives = 237/267 (88.76%), Query Frame = 0

Query: 1   MKLEVDIISEELIKPSTPTPPHLRHYPLSFIDQITVHIYAPALYFYRSNDP----EDLDF 60
           MKLEVDIISEELIKPSTPTPPHLRHY LSFIDQITVHIYAPALYFY+S D     EDLDF
Sbjct: 1   MKLEVDIISEELIKPSTPTPPHLRHYSLSFIDQITVHIYAPALYFYQSTDTGVGGEDLDF 60

Query: 61  ALAFKNRLRSSLPVVLSRYPPLAGRPNYASSFIDCNDTGVPFLEARVNSRLADVVHLARP 120
           ALAFKNRLRS+LP VLS YPPLAGRPNYASSFIDCNDTGVPF EA VNS+LADV+  A+P
Sbjct: 61  ALAFKNRLRSTLPDVLSHYPPLAGRPNYASSFIDCNDTGVPFREALVNSQLADVIQFAQP 120

Query: 121 DDLNRLFPVELDRPNEELMAVQFTEFACGGVALASCISHKIADAMTLFSLNNNWAAVARG 180
           DDLNRLFPVELDR NEELMAVQFTEFACGGVA+ASCISHKIADAMTLFSLNNNWAA+ARG
Sbjct: 121 DDLNRLFPVELDRFNEELMAVQFTEFACGGVAVASCISHKIADAMTLFSLNNNWAAMARG 180

Query: 181 AKGGVWKPHMEGGKLFPAKTMSYDTSMTIVRNRVARRFVFEGSKVEAIRGKYAESGAMGN 240
            K GV+KPHMEG K+FP K MSYD++MTIVRNRV+RRFVF+ SKVEAIR KY E+  M N
Sbjct: 181 VK-GVFKPHMEGAKIFPPKPMSYDSAMTIVRNRVSRRFVFKQSKVEAIRTKYTENQTMIN 240

Query: 241 QT-RPSRVEALSAFVYSRFLAAVKDGS 263
           Q  +PSR+E+L+AFVYSRFLAA K  S
Sbjct: 241 QNHQPSRIESLTAFVYSRFLAAFKHDS 266

BLAST of Lag0004688 vs. ExPASy TrEMBL
Match: A0A6J1KPC8 (vinorine synthase-like OS=Cucurbita maxima OX=3661 GN=LOC111496400 PE=3 SV=1)

HSP 1 Score: 423.7 bits (1088), Expect = 5.9e-115
Identity = 212/271 (78.23%), Postives = 240/271 (88.56%), Query Frame = 0

Query: 1   MKLEVDIISEELIKPSTPTPPHLRHYPLSFIDQITVHIYAPALYFYRSNDPEDLDFALAF 60
           MKLE+DIISE+LIKP+TPTPPHLRHYPLSFIDQITVHIYAPALYFYR     D DFA+ F
Sbjct: 1   MKLEIDIISEDLIKPATPTPPHLRHYPLSFIDQITVHIYAPALYFYRPTHAGDPDFAIHF 60

Query: 61  KNRLRSSLPVVLSRYPPLAGRPNYASSFIDCNDTGVPFLEARVNSRLADVVHLARPDDLN 120
           +NRLRS+L +VLS YPPLAGRPNY S+F+DCNDTG  F EARVN++LADVV  A+PDDLN
Sbjct: 61  RNRLRSTLSLVLSHYPPLAGRPNYDSAFVDCNDTGALFREARVNTKLADVVEYAQPDDLN 120

Query: 121 RLFPVELDRPNEELMAVQFTEFACGGVALASCISHKIADAMTLFSLNNNWAAVARGAKGG 180
           RLFPVELD+ NEELMAVQFTEFACGGVA+ASCISHKIADAMTLFSLNNNWAA+ARG K G
Sbjct: 121 RLFPVELDQFNEELMAVQFTEFACGGVAVASCISHKIADAMTLFSLNNNWAAMARGVK-G 180

Query: 181 VWKPHMEGGKLFPAKTMSYDTSMTIVRNRVARRFVFEGSKVEAIRGKYAESGAMGNQTRP 240
           V+KPHMEG KLFPAKT  YDT+ TI+RNRVARRFVFEGSKVEAIR KY E+GA     RP
Sbjct: 181 VFKPHMEGAKLFPAKTTRYDTAKTILRNRVARRFVFEGSKVEAIREKYGENGA---AHRP 240

Query: 241 SRVEALSAFVYSRFLAAVKDG-SSQLPTDRS 271
           SRVE+L+AF+Y RFLAA+K+G SS++P+DR+
Sbjct: 241 SRVESLTAFLYGRFLAALKEGSSSEVPSDRA 267

BLAST of Lag0004688 vs. TAIR 10
Match: AT1G24430.1 (HXXXD-type acyl-transferase family protein )

HSP 1 Score: 166.0 bits (419), Expect = 4.3e-41
Identity = 99/268 (36.94%), Postives = 154/268 (57.46%), Query Frame = 0

Query: 1   MKLEVDIISEELIKPSTPTPPHLRHYP----LSFIDQITVHIYAPALYFYRS----NDPE 60
           M LE+ + S+EL+KPS   P +L H P    LSF+DQ+   I+ P L+FY +    +D E
Sbjct: 1   MGLEITVTSQELVKPS---PRNLNHPPCHHHLSFLDQLAPPIFMPFLFFYHNKTNLSDKE 60

Query: 61  DLDFALAFKNRLRSSLPVVLSRYPPLAGRPNYASSFIDCNDTGVPFLEARVNSRLADVVH 120
             D        ++SSL  +L+ Y PLAGR   +   + CND GV F+EA+ +  ++ ++ 
Sbjct: 61  RSD-------HIKSSLSEILNLYYPLAGRIKNSGDVVVCNDVGVSFVEAKADCNMSQILE 120

Query: 121 LARPDDLNRLFPVELDRPNEELMAVQFTEFACGGVALASCISHKIADAMTLFSLNNNWAA 180
              P++LN+L P E    ++  + VQ T F CGG+AL   +SHK+ DA++     N+WAA
Sbjct: 121 NPNPNELNKLHPFEFHEVSDVPLTVQLTFFECGGLALGIGLSHKLCDALSGLIFVNSWAA 180

Query: 181 VARGAKGGVWKPHMEGGKLFP---AKTMSYDTSMTIVRNRVARRFVFEGSKVEAIRGKYA 240
            ARG    +  P  +  K+FP    + ++  T +T   N V RRFVF  S VE++R +++
Sbjct: 181 FARGQTDEIITPSFDLAKMFPPCDIENLNMATGIT-KENIVTRRFVFLRSSVESLRERFS 240

Query: 241 ESGAMGNQTRPSRVEALSAFVYSRFLAA 258
            +     + R +RVE LS F++SRF+A+
Sbjct: 241 GN----KKIRATRVEVLSVFIWSRFMAS 253

BLAST of Lag0004688 vs. TAIR 10
Match: AT3G26040.1 (HXXXD-type acyl-transferase family protein )

HSP 1 Score: 153.7 bits (387), Expect = 2.2e-37
Identity = 88/266 (33.08%), Postives = 149/266 (56.02%), Query Frame = 0

Query: 3   LEVDIISEELIKPSTPTPPHLRHYPLSFIDQITVHIYAPALYFYRSNDPEDLDFALAFKN 62
           + VD++S ++IKPS+PTP HL+ + LS ++Q+   I+ P ++FY +N+       L    
Sbjct: 1   MRVDVVSRDIIKPSSPTPNHLKKFKLSLLEQLGPTIFGPMVFFYSANNSIKPTEQL---Q 60

Query: 63  RLRSSLPVVLSRYPPLAGRPNYASSFIDCNDTGVPFLEARVNSRLADVVHLARPDDLNRL 122
            L+ SL   L+ + PLAGR    +  IDCND+G  FLEARVNS L++++     D L +L
Sbjct: 61  MLKKSLSETLTHFYPLAGRLK-GNISIDCNDSGADFLEARVNSPLSNLLLEPSSDSLQQL 120

Query: 123 FPVELD--RPNEELMAVQFTEFACGGVALASCISHKIADAMTLFSLNNNWAAV-ARGAKG 182
            P  +D       L+  Q + F CG +++  CISHK+ADA ++     +WAA+ +RG+  
Sbjct: 121 IPTSVDSIETRTRLLLAQASFFECGSMSIGVCISHKLADATSIGLFMKSWAAISSRGSIK 180

Query: 183 GVWKPHMEGGKLFPAKTMSYDTSMTIVRNRV------ARRFVFEGSKVEAIRGKYAESGA 242
            +  P  +  K+FP    S  +   +V   +      ++RF+F+ S ++A++ K     +
Sbjct: 181 TIGAPVFDTVKIFPPGNFSETSPAPVVEPEIMMNQTLSKRFIFDSSSIQALQAK----AS 240

Query: 243 MGNQTRPSRVEALSAFVYSRFLAAVK 260
                +P+RVEA+SA ++   + A +
Sbjct: 241 SFEVNQPTRVEAVSALIWKSAMKATR 258

BLAST of Lag0004688 vs. TAIR 10
Match: AT4G15390.1 (HXXXD-type acyl-transferase family protein )

HSP 1 Score: 147.5 bits (371), Expect = 1.6e-35
Identity = 94/271 (34.69%), Postives = 148/271 (54.61%), Query Frame = 0

Query: 1   MKLEVDIISEELIKPSTPTPPHLRHYPLSFIDQITVHIYAPALYFYRSNDPEDLDFALAF 60
           M ++V+ IS+E+IKPS+PTP +L+   LS  D I   +Y  A  FY  ND    +     
Sbjct: 4   MTMKVETISKEIIKPSSPTPNNLQTLQLSIYDHILPPVYTVAFLFYTKNDLISQEHT--- 63

Query: 61  KNRLRSSLPVVLSRYPPLAGRPNYASSFIDCNDTGVPFLEARVNS-RLADVVHLARPDDL 120
            ++L++SL   L+++ PLAGR       +DC D G  F++ARVN+  L + +     D L
Sbjct: 64  SHKLKTSLSETLTKFYPLAGR--ITGVTVDCTDEGAIFVDARVNNCPLTEFLKCPDFDAL 123

Query: 121 NRLFPVE-LDRP-----NEELMAVQFTEFACGGVALASCISHKIADAMTLFSLNNNWAAV 180
            +L P++ +D P        L+ V+ T F CGG+A+  CI+HKIADA ++ +   +WAA 
Sbjct: 124 QQLLPLDVVDNPYVAAATWPLLLVKATYFGCGGMAIGICITHKIADAASISTFIRSWAAT 183

Query: 181 ARGAK--GGVWKPHMEGGKLFPAKTMSYD---TSMTIVRNRVARRFVFEGSKVEAIRGKY 240
           ARG      +  P   G   +P    ++          R+ + +RFVFE SKVE +R K 
Sbjct: 184 ARGENDAAAMESPVFAGANFYPPANEAFKLPADEQAGKRSSITKRFVFEASKVEDLRTKA 243

Query: 241 AESGAMGNQTRPSRVEALSAFVYSRFLAAVK 260
           A    +    +P+RVE+++A ++  F+A+ K
Sbjct: 244 ASEETV---DQPTRVESVTALIWKCFVASSK 266

BLAST of Lag0004688 vs. TAIR 10
Match: AT3G30280.1 (HXXXD-type acyl-transferase family protein )

HSP 1 Score: 144.1 bits (362), Expect = 1.7e-34
Identity = 95/272 (34.93%), Postives = 150/272 (55.15%), Query Frame = 0

Query: 3   LEVDIISEELIKPSTPTPPHLRHYPLSFIDQITVHIYAPALYFYRSNDPEDLDFALAFKN 62
           ++V+ I++E+IKPS+ TP  L+   LS  D I   +Y  A  FY     +DL       +
Sbjct: 4   MKVETIAKEIIKPSSTTPNDLQTLQLSIYDHILPPVYTVAFLFYTK---DDLISPEQSSH 63

Query: 63  RLRSSLPVVLSRYPPLAGRPNYASSFIDCNDTGVPFLEARVNS-RLADVVHLARPDDLNR 122
           +L++SL   L+++ PLAGR    +  IDCND G  F++ARVN+  L+D +       L +
Sbjct: 64  KLKTSLAETLTKFYPLAGRIKGVT--IDCNDEGAVFVDARVNNYPLSDFLRSPDFKTLQQ 123

Query: 123 LFPVE-LDRPNE-----ELMAVQFTEFACGGVALASCISHKIADAMTLFSLNNNWAAVAR 182
           L P++ +D P E      L+ V+ T F CGG+A+  CI+HKIADA ++ +   +WAA+AR
Sbjct: 124 LLPLDVIDDPYEAATTWPLLLVKATYFPCGGMAIGLCITHKIADATSISTFIQSWAAMAR 183

Query: 183 GAKGG-VWKPHMEGGKLFPAKTMSYDTSMTIVRNRVA---RRFVFEGSKVEAIRGKYAES 242
           G  G  V  P       +P     +   +    N+++   +RFVF  SK+E +R K A  
Sbjct: 184 GEAGDIVADPVFAAANFYPPANELFKFPVDENANKISQITKRFVFSASKLEELRTKAASE 243

Query: 243 GAMGNQTRPSRVEALSAFVYSRFLAAVKDGSS 264
             +G   RP RVE+++A ++  F+AA    ++
Sbjct: 244 DFVG---RPKRVESVTALLWKAFVAAASSNTN 267

BLAST of Lag0004688 vs. TAIR 10
Match: AT5G47950.1 (HXXXD-type acyl-transferase family protein )

HSP 1 Score: 137.9 bits (346), Expect = 1.2e-32
Identity = 92/261 (35.25%), Postives = 138/261 (52.87%), Query Frame = 0

Query: 3   LEVDIISEELIKPSTPTPPHLRHYPLSFIDQITVHIYAPALYFYRSNDPEDLDFALAFKN 62
           ++V+ I +E+IKPS  TP  L    LS +D +   +YA A  FY     +DL       +
Sbjct: 4   MKVETIGKEIIKPSATTPTDLSTLQLSIMDILMPPVYAVAFLFYTK---DDLISQEQTSH 63

Query: 63  RLRSSLPVVLSRYPPLAGRPNYASSFIDCNDTGVPFLEARV-NSRLADVVHLARPDDLNR 122
            L++SL  +L+++ PLAGR N  +  I   D G  F+EARV N  L+  +     + L +
Sbjct: 64  TLKTSLSEILTKFHPLAGRVNGVT--IKSTDEGAVFVEARVDNCDLSGFLRSPDTESLKQ 123

Query: 123 LFPVELD-RPNEELMAVQFTEFACGGVALASCISHKIADAMTLFSLNNNWAAVARGAKGG 182
           L PV+ +  P   L+ V+ T F CGG+A+  CISH++ADA +L      WAA ARG    
Sbjct: 124 LLPVDDEPAPTWPLLLVKATYFRCGGMAIGLCISHRLADAASLSIFLQAWAATARGESDL 183

Query: 183 VWKPHMEGGKLFPAKTMSYDTSMTIV-RNRVARRFVFEGSKVEAIRGKYAESGAMGNQTR 242
           V  P     KL+PA   +      +V R  V +RFVF  SK+E +R K A         R
Sbjct: 184 VASPDFCSTKLYPAANEAIKIPGEVVNRTSVTKRFVFVASKIEELRNKVASDVV----PR 243

Query: 243 PSRVEALSAFVYSRFLAAVKD 261
           P+RV+++++ ++   + A  D
Sbjct: 244 PTRVQSVTSLIWKCVVTASTD 255

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
XP_038886173.18.1e-11983.15stemmadenine O-acetyltransferase-like [Benincasa hispida][more]
XP_008445555.16.9e-11882.02PREDICTED: vinorine synthase-like [Cucumis melo][more]
KAA0061032.16.9e-11882.02vinorine synthase-like [Cucumis melo var. makuwa][more]
XP_022951641.12.2e-11679.70vinorine synthase-like [Cucurbita moschata][more]
XP_004143048.13.8e-11682.02stemmadenine O-acetyltransferase [Cucumis sativus] >KGN62351.1 hypothetical prot... [more]
Match NameE-valueIdentityDescription
A0A2P1GIW72.5e-3837.26Stemmadenine O-acetyltransferase OS=Catharanthus roseus OX=4058 GN=SAT PE=1 SV=1[more]
Q70PR72.7e-3234.73Vinorine synthase OS=Rauvolfia serpentina OX=4060 GN=ACT PE=1 SV=2[more]
A0A2G3D6U16.6e-3132.39Acyltransferase Pun1 OS=Capsicum chinense OX=80379 GN=PUN1 PE=2 SV=1[more]
Q8GZU05.6e-3033.85Minovincinine 19-hydroxy-O-acetyltransferase OS=Catharanthus roseus OX=4058 GN=M... [more]
D2Y3X29.6e-3031.82Acyltransferase Pun1 OS=Capsicum annuum OX=4072 GN=PUN1 PE=1 SV=1[more]
Match NameE-valueIdentityDescription
A0A5A7UYQ73.3e-11882.02Vinorine synthase-like OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold5... [more]
A0A1S3BD013.3e-11882.02vinorine synthase-like OS=Cucumis melo OX=3656 GN=LOC103488534 PE=3 SV=1[more]
A0A6J1GI321.1e-11679.70vinorine synthase-like OS=Cucurbita moschata OX=3662 GN=LOC111454395 PE=3 SV=1[more]
A0A0A0LQS81.8e-11682.02Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_2G350340 PE=3 SV=1[more]
A0A6J1KPC85.9e-11578.23vinorine synthase-like OS=Cucurbita maxima OX=3661 GN=LOC111496400 PE=3 SV=1[more]
Match NameE-valueIdentityDescription
AT1G24430.14.3e-4136.94HXXXD-type acyl-transferase family protein [more]
AT3G26040.12.2e-3733.08HXXXD-type acyl-transferase family protein [more]
AT4G15390.11.6e-3534.69HXXXD-type acyl-transferase family protein [more]
AT3G30280.11.7e-3434.93HXXXD-type acyl-transferase family protein [more]
AT5G47950.11.2e-3235.25HXXXD-type acyl-transferase family protein [more]
InterPro
Analysis Name: InterPro Annotations of Sponge gourd (AG-4) v1
Date Performed: 2022-08-01
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR023213Chloramphenicol acetyltransferase-like domain superfamilyGENE3D3.30.559.10coord: 206..273
e-value: 3.5E-7
score: 31.5
IPR023213Chloramphenicol acetyltransferase-like domain superfamilyGENE3D3.30.559.10coord: 1..202
e-value: 4.4E-55
score: 188.3
IPR003480TransferasePFAMPF02458Transferasecoord: 4..262
e-value: 5.0E-46
score: 157.2
NoneNo IPR availablePANTHERPTHR31623:SF46ANTHRANILATE N-BENZOYLTRANSFERASEcoord: 2..262
NoneNo IPR availablePANTHERPTHR31623F21J9.9coord: 2..262

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Lag0004688.1Lag0004688.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
molecular_function GO:0016747 acyltransferase activity, transferring groups other than amino-acyl groups