Lag0004659 (gene) Sponge gourd (AG‐4) v1

Overview
NameLag0004659
Typegene
OrganismLuffa acutangula (Sponge gourd (AG‐4) v1)
Descriptioneukaryotic translation initiation factor 4B2-like
Locationchr6: 5857859 .. 5862681 (-)
RNA-Seq ExpressionLag0004659
SyntenyLag0004659
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: polypeptideexonCDS
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGTCAAAACCCTGGGGCGGAATCGGAGCCTGGGCTGCTGATGCCGAGCGTGCCGAGGCTGAGGAACGAGAGGCGCAAGCTGCGGCCGCTGCCTCCCAAGCAGAATCCAAGAATTTCCCTAGCCTTAGGGAAGCTGTCAACACCAAGCCCAAGAAGAAGAAGATGACGCTCTCGGAATTCACTATGGGGACCTTGACGGGTCCGGGCGCCGGCAGTAGGCAAACGGAGAGTCAGGGATTGACGCCGGAGGAGATGCTTCGGCTTCCGACTGGTCCCAAAGACCGTTCGGGTGAGGAAATGCGGTCTGGCCGGCTCGGTGGGGGATTTCCCAGTTACGATCGGCAGAGTATGCCTGCTGGTCGGTTTCGAGACCGTGATGAAGGCGATGGGTCTTGGGGTGGTGGTCGACGATCGTATGGTGGGTTCGACGAGGAACGCAGAGGTCCACCTCCTAGGGCTTCCAATTTCGATCAACCTTCGAGGGCTGACGAGGTGGATAATTGGGCAATGACGAAGAAATCTCCCACATTGGATTCAGGGCGACAAAACAGGTATGGGTCGCTAGGCGGTGGCATGGGTGGGATTGGTGGTGGTGGGATTGCATCTAGGGCTGACGAGGTCGATAACTGGGCAAGTTCGAAAAAGGCTATTCCATCGAGATCCTCCACATTTGGTTCTGGTTTCCGTGACCGTGAGGCGAATGAGCCCGATCGCTGGACTAGAGGCCGTTCACGTGATAGTGACACAGAACGACCCCGATTAGTACTGGATCCACCCAAAGGTAATGTCGTTACAAATGAAGCAACAAGGACGAACAAGCCAAGTCCTTTTGGAGCAGCAAGGCCAAGAGAGGAGGTTTTGGCTGAGAAGGGGTTGGACTGGAAGAAGCTGGATACAGAAATTGAGGCCAAAAGAACCAGTAGGCCAACCAGTGCTCACTCTAGCAGGCCTTCGAGTGCTCAATCCAGCCGTTCGGAGGGTCCAGGAATACAGGGCGTAGAAAATGTGGTGAAGCCGAGGCCAAAAGTCAATCCATTTGGGGATGCCAAACCTAGGGAAGTTTTGCTGGAGGAACGTGGCAAGGACTGGCGAAAGATAGATCTGCAACTTGAGCATCGTGCTGTCGACAGGTCCTGATTTCACTTTTCACATTGTTAATCTCTATCTCTCTCTTATTCTTATGCTTAGGTGCAGGGGATAATTTCTTTGTTTTCTGATTGTTATTTGGTTCTTGGAGTCAAATGTTTCCGGCATGAAGCTAGCTGGAGCTGTTCTATTGAGGATTAGGTTTAACTTGATTTTTCACTTTGCAAAGGTTTTGACAGCGAGCTGCCTATGTCATTAGCTGAGTAGAGCTATTTTTTGAGTAAACTTGCTAGTAATTTATGTTTATGTTAAAAACTATTGTTAGGACCTGTGGAACAAAATTATCAAGGACTAGGAAATGATGACCTAGCTATAATCTTAGAATTGGAACTTGAGTGAACATGTATGTCATACAGTTCTATCCAGCTTGTTTACTTTGCTGCTTGAGTCATTGCCTAATAAATGATTATCATCAGGAAACAACAAAAGAAAAAATGACAGAAGATAATTTTCTAATATGATCACTTTTGCTTTAGAGTTTTTGGCATTCAGTTAGAATTTTAGTTTAAATCCTATTTGCCACATGAGCTCTCGTATGTTAGTTTATTTTGGTCCTTATGTTTTCTACTTTTGATTTATGAAGTTTGCTTTTTGCGGCTAAATTTTGATGTTGTTTTTGAGTTTTGACATAAACCTGAGGCCCCCTATTTCTAAACCATCAGACTAATAAATATAGCTTAAAGCACTAGTCTACGTAATGCCACACCAATTTGCTTGTAATAAATGCAATTTGGAAATTTAGAGATACACACACATGGACACACAAACGAATTGATTAAATACAAGAAAGAGGAAATACAAAAATGAAGATAGAGACTAGAGAGCATGAAATAGGACTAAATTTGCATCAAACCTTAATTGCTAAAGTCAATATTAAGAGAGCATGAAATAGGACTAGATTCTGAAGATCATCACGAAAAGAGTCACTTCCTTAAAAGATCCTCTTCTTTCTTTCATACTAATTACTAAAGACCCTCAGTTGGCATAAATTTGCATCAAACCTTAATTACAAAAGTCAATATGAAGAGAGCATCAACTAGAAGAAAACCGTCTGACTTTGATCTTTACCTTAGAAAATTCTTATATTTCTTTCATTCCAAATCAACCCAATTGTTTGCTCTTGTGATGTGAAGCCACAAAATCTTCTTGGACTGTCTGAATGGATGGTATTTGAGAAGTAAATTCATCCAGTCAAAAACTTCATAGGGGAGAACCACACTCCAATCAAAGAAAATAAATAGATTGGCCCAAATATTTGAGGAGAAAGGGCAGTGGATAAAAATGTGTCTTTGTGATTCATTTGCTTCTTTACACAAAACACTAATTTGGAGATAGTGTGACCCAAGGACACCTTCTCTGTAATCTATCATAAGTATAGGCATGATTGACTATGTTCCCAATGAAGAAATTTAATCTTCTTGGGTTAGAAGATTAAAATGCCTTCAAAAGAATTCTAGCTAAGCCTCCAAAGAAAGTCCCATGTATAAGCTGGTAAAATTCCTCCAAACAACTCTTGGGAATGACCCAGCACAAATTAAATGTTTTGAAAAAGGCTACATTCAAAATTTTAGTGATGAACACCAAATTTGATTTTTATTCTAGTCACCAAAATAGAATATTTGTAAGCATATTCACCCATATGAAATAAGTGTGAAACTTAGAAGAAAATAGGATTTAAACATGGAATTCTCTATTATAGAGAATTGTATCTAATAAAAATTCTCTAGGACATGAAACCAAAGCAAACTTTCCGTGTGAAAGCATTTAGAGCTTATTGTGCACAGCCTTTATTTATTAGTTTATTTATTTTTTAATTATTATTATTATTATTATTTTAATTTTTTTTAATAGGAAACTGATTTTTTTTTAAAAGATGTGCACAACAAGCCTCAAGTATCTTTCTATGTTGTGAAATGTTGTGCTATGATGTGGTCACAACATTTGCTTTTGTCTGTTACAACTTACATCTAGTATAGTTTGCTGCCACAAGCCTCAAGTATATCTCTCAATGCTAATTTATATGCAGGTGACCTCATCAACTCCCATGTTTATTTCAATTTGTAGCTATAGATTAGTTAAGGCATGGATGGCAATGTATCATGAGAAAGTTTTTCAACAGTTTGTACCTTTTATTTTTATTTTTATTATTTTTAAATAAGAAACAAGTGACTTTCATTCAAGGTGGTACCTAGCCAAAACAAAACAACCTAAGGGACAAAGGGATAAGACATCCCCTCTCCTGAAAGAAGGATTACAAAAAGACCTAGTCTAAATTATGTTTGTACAGTTTGATCATCAGATTCTTTTTAGCAAAAGTCGTAAAGGAATCAGAACCTTTAAATGCTCTGAGCTGTTAAGTGCTTACATCATTAGATCTCGTTTGTTGTCAAATAATTGGATGCAAAAGATCAACCATGAGAAAGTAGTTTCTCTAAACCCTACAATATGTTGATGATTAGATTTTAGGAAATGTATGTCCTTTTGCTTATAATTTGAATGCAGGGACTGAATGTTGAACCACTATGACATGAAATTATAACAGTAGGTTTTTCTTCTAGTGTATTATTTTTCTTTTCTTGTACCAGGGATCTCGAAGAGATTCTCTTTCTATCTTGTGCACTTTAAGTTTTATTGAATACATTTTTTTTTTTAAACAAAAGAAAGATGAAAAGAATTTGTTTAACGTAAGTTGATAAAGGTCATATGTGGCCCTTGGTGGCTTAATCTGCGAATGTAGGCATTTTTTCATAAATCTGTTGGACGAAGTGTAGCTTTACTTATCTATCGTGTATCACCTCAATTGAATGAGGTGAAAACATAAAAAACAAATATTTATTAGTATGCTCTGAATTGCTTGAATTTCAAGCCTTTGGGGTGGTTCTTCTCTTAACTGTGTCTACTCTTTTATCACAGGCCTGAAACAGAAGAAGAAAAGATGTTGAAAGAGGAAATTGATGTTCTAAGAAAGGAACTTGAGGAAGGCTTATCTCTGAATACTAACAAAGAATCGGTGCAAGAATCTGCTGGAGAGATGCAAGATCTGCGTGACAGAATAAGTAAAAAAGAACGTGAACTGGAGACACTGGTCCTTGATTTGGATGATAAAATTCGCTTCGGGAAGAAAGCCACTGAGAGACCTGGCTCTGGAGCAGGTAGGGCTGCCGTCTTTCCAGAAAGACCACCTTCTCAAGGATCCTTTGATGAGTCCAGAAGTTTTGAGTCTATAGACCGGCCTCGATCACGTGGCACTGGCACTGCAGATGTATGGAGTAGATCTGCTGATGATAGAAAACCAATCCAAGGTGCCAGAGATAGAGGATTTTTGGGCAACAGAGACATGGAGAGGTTTGGATTCGTCTGTCATTTTGTTCCTCTCTGTTGATTCTTGGTTCTTAAACGTTTTAATATTGTCATTACATCTGCGAGAAATATGAATTGCACATATCTGAAAATATTTAATTAGTTTTTTATACTTGGGATAAAACACCTTTTCTTACTGGCGTGAGGGTGCTGCAGAAATTAGGAGTAATATTTTGAAATTTTGCACTTGTTCATAGAAAGCTCGAATTGTTCGTTTATTCTGTTCGTTCCAAGTTACTCAGTCTTGTGAAATGTGTCATTCTGAATCTCAAATATGGCATTTGTGTTCTTATTCCAGGTCGAGGGATAGATGGTAA

mRNA sequence

ATGTCAAAACCCTGGGGCGGAATCGGAGCCTGGGCTGCTGATGCCGAGCGTGCCGAGGCTGAGGAACGAGAGGCGCAAGCTGCGGCCGCTGCCTCCCAAGCAGAATCCAAGAATTTCCCTAGCCTTAGGGAAGCTGTCAACACCAAGCCCAAGAAGAAGAAGATGACGCTCTCGGAATTCACTATGGGGACCTTGACGGGTCCGGGCGCCGGCAGTAGGCAAACGGAGAGTCAGGGATTGACGCCGGAGGAGATGCTTCGGCTTCCGACTGGTCCCAAAGACCGTTCGGGTGAGGAAATGCGGTCTGGCCGGCTCGGTGGGGGATTTCCCAGTTACGATCGGCAGAGTATGCCTGCTGGTCGGTTTCGAGACCGTGATGAAGGCGATGGGTCTTGGGGTGGTGGTCGACGATCGTATGGTGGGTTCGACGAGGAACGCAGAGGTCCACCTCCTAGGGCTTCCAATTTCGATCAACCTTCGAGGGCTGACGAGGTGGATAATTGGGCAATGACGAAGAAATCTCCCACATTGGATTCAGGGCGACAAAACAGGTATGGGTCGCTAGGCGGTGGCATGGGTGGGATTGGTGGTGGTGGGATTGCATCTAGGGCTGACGAGGTCGATAACTGGGCAAGTTCGAAAAAGGCTATTCCATCGAGATCCTCCACATTTGGTTCTGGTTTCCGTGACCGTGAGGCGAATGAGCCCGATCGCTGGACTAGAGGCCGTTCACGTGATAGTGACACAGAACGACCCCGATTAGTACTGGATCCACCCAAAGGTAATGTCGTTACAAATGAAGCAACAAGGACGAACAAGCCAAGTCCTTTTGGAGCAGCAAGGCCAAGAGAGGAGGTTTTGGCTGAGAAGGGGTTGGACTGGAAGAAGCTGGATACAGAAATTGAGGCCAAAAGAACCAGTAGGCCAACCAGTGCTCACTCTAGCAGGCCTTCGAGTGCTCAATCCAGCCGTTCGGAGGGTCCAGGAATACAGGGCGTAGAAAATGTGGTGAAGCCGAGGCCAAAAGTCAATCCATTTGGGGATGCCAAACCTAGGGAAGTTTTGCTGGAGGAACGTGGCAAGGACTGGCGAAAGATAGATCTGCAACTTGAGCATCGTGCTGTCGACAGGCCTGAAACAGAAGAAGAAAAGATGTTGAAAGAGGAAATTGATGTTCTAAGAAAGGAACTTGAGGAAGGCTTATCTCTGAATACTAACAAAGAATCGGTGCAAGAATCTGCTGGAGAGATGCAAGATCTGCGTGACAGAATAAGTAAAAAAGAACGTGAACTGGAGACACTGGTCCTTGATTTGGATGATAAAATTCGCTTCGGGAAGAAAGCCACTGAGAGACCTGGCTCTGGAGCAGGTAGGGCTGCCGTCTTTCCAGAAAGACCACCTTCTCAAGGATCCTTTGATGAGTCCAGAAGTTTTGAGTCTATAGACCGGCCTCGATCACGTGGCACTGGCACTGCAGATGTATGGAGTAGATCTGCTGATGATAGAAAACCAATCCAAGGTGCCAGAGATAGAGGATTTTTGGGCAACAGAGACATGGAGAGGTCGAGGGATAGATGGTAA

Coding sequence (CDS)

ATGTCAAAACCCTGGGGCGGAATCGGAGCCTGGGCTGCTGATGCCGAGCGTGCCGAGGCTGAGGAACGAGAGGCGCAAGCTGCGGCCGCTGCCTCCCAAGCAGAATCCAAGAATTTCCCTAGCCTTAGGGAAGCTGTCAACACCAAGCCCAAGAAGAAGAAGATGACGCTCTCGGAATTCACTATGGGGACCTTGACGGGTCCGGGCGCCGGCAGTAGGCAAACGGAGAGTCAGGGATTGACGCCGGAGGAGATGCTTCGGCTTCCGACTGGTCCCAAAGACCGTTCGGGTGAGGAAATGCGGTCTGGCCGGCTCGGTGGGGGATTTCCCAGTTACGATCGGCAGAGTATGCCTGCTGGTCGGTTTCGAGACCGTGATGAAGGCGATGGGTCTTGGGGTGGTGGTCGACGATCGTATGGTGGGTTCGACGAGGAACGCAGAGGTCCACCTCCTAGGGCTTCCAATTTCGATCAACCTTCGAGGGCTGACGAGGTGGATAATTGGGCAATGACGAAGAAATCTCCCACATTGGATTCAGGGCGACAAAACAGGTATGGGTCGCTAGGCGGTGGCATGGGTGGGATTGGTGGTGGTGGGATTGCATCTAGGGCTGACGAGGTCGATAACTGGGCAAGTTCGAAAAAGGCTATTCCATCGAGATCCTCCACATTTGGTTCTGGTTTCCGTGACCGTGAGGCGAATGAGCCCGATCGCTGGACTAGAGGCCGTTCACGTGATAGTGACACAGAACGACCCCGATTAGTACTGGATCCACCCAAAGGTAATGTCGTTACAAATGAAGCAACAAGGACGAACAAGCCAAGTCCTTTTGGAGCAGCAAGGCCAAGAGAGGAGGTTTTGGCTGAGAAGGGGTTGGACTGGAAGAAGCTGGATACAGAAATTGAGGCCAAAAGAACCAGTAGGCCAACCAGTGCTCACTCTAGCAGGCCTTCGAGTGCTCAATCCAGCCGTTCGGAGGGTCCAGGAATACAGGGCGTAGAAAATGTGGTGAAGCCGAGGCCAAAAGTCAATCCATTTGGGGATGCCAAACCTAGGGAAGTTTTGCTGGAGGAACGTGGCAAGGACTGGCGAAAGATAGATCTGCAACTTGAGCATCGTGCTGTCGACAGGCCTGAAACAGAAGAAGAAAAGATGTTGAAAGAGGAAATTGATGTTCTAAGAAAGGAACTTGAGGAAGGCTTATCTCTGAATACTAACAAAGAATCGGTGCAAGAATCTGCTGGAGAGATGCAAGATCTGCGTGACAGAATAAGTAAAAAAGAACGTGAACTGGAGACACTGGTCCTTGATTTGGATGATAAAATTCGCTTCGGGAAGAAAGCCACTGAGAGACCTGGCTCTGGAGCAGGTAGGGCTGCCGTCTTTCCAGAAAGACCACCTTCTCAAGGATCCTTTGATGAGTCCAGAAGTTTTGAGTCTATAGACCGGCCTCGATCACGTGGCACTGGCACTGCAGATGTATGGAGTAGATCTGCTGATGATAGAAAACCAATCCAAGGTGCCAGAGATAGAGGATTTTTGGGCAACAGAGACATGGAGAGGTCGAGGGATAGATGGTAA

Protein sequence

MSKPWGGIGAWAADAERAEAEEREAQAAAAASQAESKNFPSLREAVNTKPKKKKMTLSEFTMGTLTGPGAGSRQTESQGLTPEEMLRLPTGPKDRSGEEMRSGRLGGGFPSYDRQSMPAGRFRDRDEGDGSWGGGRRSYGGFDEERRGPPPRASNFDQPSRADEVDNWAMTKKSPTLDSGRQNRYGSLGGGMGGIGGGGIASRADEVDNWASSKKAIPSRSSTFGSGFRDREANEPDRWTRGRSRDSDTERPRLVLDPPKGNVVTNEATRTNKPSPFGAARPREEVLAEKGLDWKKLDTEIEAKRTSRPTSAHSSRPSSAQSSRSEGPGIQGVENVVKPRPKVNPFGDAKPREVLLEERGKDWRKIDLQLEHRAVDRPETEEEKMLKEEIDVLRKELEEGLSLNTNKESVQESAGEMQDLRDRISKKERELETLVLDLDDKIRFGKKATERPGSGAGRAAVFPERPPSQGSFDESRSFESIDRPRSRGTGTADVWSRSADDRKPIQGARDRGFLGNRDMERSRDRW
Homology
BLAST of Lag0004659 vs. NCBI nr
Match: XP_011649497.1 (eukaryotic translation initiation factor 4B2 [Cucumis sativus] >KGN62328.1 hypothetical protein Csa_018560 [Cucumis sativus])

HSP 1 Score: 916.8 bits (2368), Expect = 8.7e-263
Identity = 486/540 (90.00%), Postives = 502/540 (92.96%), Query Frame = 0

Query: 1   MSKPWGGIGAWAADAERAEAEEREAQAAAAASQAESKNFPSLREAVNTKPKKKKMTLSEF 60
           MSKPWGGIGAWAADAERAEAEEREAQAAAAASQ+ESKNFPSLREAV+TKPKKKKM+LSEF
Sbjct: 1   MSKPWGGIGAWAADAERAEAEEREAQAAAAASQSESKNFPSLREAVSTKPKKKKMSLSEF 60

Query: 61  TMGTLTGPGAGSRQTESQGLTPEEMLRLPTGPKDRSGEEMRSGRLGGGFPSYDRQSMPAG 120
           TMGTLTGPG G R T++QGLTPEEM+RLPTGPKDRSGEE+RSGRLGGGFP+YDRQ M AG
Sbjct: 61  TMGTLTGPGTGGRLTDTQGLTPEEMIRLPTGPKDRSGEEVRSGRLGGGFPTYDRQGMSAG 120

Query: 121 RFRDRDEGDGSWGGGRRSYGGFDEERRGPPPRASNFDQPSRADEVDNWAMTKKSPTLDSG 180
           RFRDRDEGDGSWGGGRRSYGGFDEERRGP  RASNFDQPSRADEVDNWAMTKKSPTLDSG
Sbjct: 121 RFRDRDEGDGSWGGGRRSYGGFDEERRGPSSRASNFDQPSRADEVDNWAMTKKSPTLDSG 180

Query: 181 RQNRYGSLGGGMGGIGGGGIASRADEVDNWASSKKAIPSRSSTFGSGFRDREANEPDRWT 240
           RQNRYGSLGGG+     GGIASRADEVDNWAS KK IPSRSSTFGSG+  REANEPDRW 
Sbjct: 181 RQNRYGSLGGGV-----GGIASRADEVDNWASVKKPIPSRSSTFGSGY--REANEPDRWG 240

Query: 241 RGRSRDSDTERPRLVLDPPKGNVVTNEATRTNKPSPFGAARPREEVLAEKGLDWKKLDTE 300
           RGRSRDSDTERPRLVLDPPKGNV TNEATRTNKPSPFGAARPREEVLAEKGLDWKKLDTE
Sbjct: 241 RGRSRDSDTERPRLVLDPPKGNVATNEATRTNKPSPFGAARPREEVLAEKGLDWKKLDTE 300

Query: 301 IEAKRTSRPTSAHSSRPSSAQSSRSEGPGIQGVENVVKPRPKVNPFGDAKPREVLLEERG 360
           IEAKRTSRPTSAHSSRPSSAQSSRSEGPG+QG+ENV KPRPKVNPFGDAKPREVLLEERG
Sbjct: 301 IEAKRTSRPTSAHSSRPSSAQSSRSEGPGLQGLENVAKPRPKVNPFGDAKPREVLLEERG 360

Query: 361 KDWRKIDLQLEHRAVDRPETEEEKMLKEEIDVLRKELEEGLSLNTNKESVQESAGEMQDL 420
           KDWRKIDLQLEHRAVDRPETEEEKMLKEEIDVLRKELEEGLSLN NKESVQESAGEMQ+L
Sbjct: 361 KDWRKIDLQLEHRAVDRPETEEEKMLKEEIDVLRKELEEGLSLNANKESVQESAGEMQNL 420

Query: 421 RDRISKKERELETLVLDLDDKIRFGKKATERPGSGAGRAA--------------VFPERP 480
           RDRI+KKE ELETLVLDLDDK+RFGKKATERPGSGAGRAA              VFPERP
Sbjct: 421 RDRINKKEHELETLVLDLDDKVRFGKKATERPGSGAGRAAVFPERPGSGAGRATVFPERP 480

Query: 481 PSQGSFDESRSFESIDRPRSRGTGTADVWSRSADDRKPIQGARDRGFLGNRDMERSRDRW 527
           PSQGSFDESRSFES+DRPRSR  GTADVWSR ADDRKPIQGARDRGFLGNRDMERSRDRW
Sbjct: 481 PSQGSFDESRSFESVDRPRSR--GTADVWSRPADDRKPIQGARDRGFLGNRDMERSRDRW 531

BLAST of Lag0004659 vs. NCBI nr
Match: XP_038884361.1 (eukaryotic translation initiation factor 4B2-like [Benincasa hispida])

HSP 1 Score: 912.1 bits (2356), Expect = 2.1e-261
Identity = 485/540 (89.81%), Postives = 500/540 (92.59%), Query Frame = 0

Query: 1   MSKPWGGIGAWAADAERAEAEEREAQAAAAASQAESKNFPSLREAVNTKPKKKKMTLSEF 60
           MSKPWGGIGAWAADAERAEAEEREAQAAAAASQAESKNFPSLREAV+TKPKKKKM+LSEF
Sbjct: 1   MSKPWGGIGAWAADAERAEAEEREAQAAAAASQAESKNFPSLREAVSTKPKKKKMSLSEF 60

Query: 61  TMGTLTGPGAGSRQTESQGLTPEEMLRLPTGPKDRSGEEMRSGRLGGGFPSYDRQSMPAG 120
           TMGTLTGPGAG RQTESQGLTPEEM+RLPTGPKDRSGEE+RSGRLGGGFP+YDR +M AG
Sbjct: 61  TMGTLTGPGAGGRQTESQGLTPEEMIRLPTGPKDRSGEEVRSGRLGGGFPTYDRNNMSAG 120

Query: 121 RFRDRDEGDGSWGGGRRSYGGFDEERRGPPPRASNFDQPSRADEVDNWAMTKKSPTLDSG 180
           R+RDRDEGDGSWG GRRSYGGFDEERRGP PRASNFDQPSRADEVDNWAMTKKSPTLDSG
Sbjct: 121 RYRDRDEGDGSWGSGRRSYGGFDEERRGPSPRASNFDQPSRADEVDNWAMTKKSPTLDSG 180

Query: 181 RQNRYGSLGGGMGGIGGGGIASRADEVDNWASSKKAIPSRSSTFGSGFRDREANEPDRWT 240
           RQNRYGSLGGG+     GGIASRADEVDNWAS KK IPSRSSTFGSG+  REANEPDRW 
Sbjct: 181 RQNRYGSLGGGV-----GGIASRADEVDNWASVKKPIPSRSSTFGSGY--REANEPDRWG 240

Query: 241 RGRSRDSDTERPRLVLDPPKGNVVTNEATRTNKPSPFGAARPREEVLAEKGLDWKKLDTE 300
           RGRSRDSDTERPRLVLDPPKGNVVTNEATRTNKPSPFGAARPREEVLAEKGLDWKKLDTE
Sbjct: 241 RGRSRDSDTERPRLVLDPPKGNVVTNEATRTNKPSPFGAARPREEVLAEKGLDWKKLDTE 300

Query: 301 IEAKRTSRPTSAHSSRPSSAQSSRSEGPGIQGVENVVKPRPKVNPFGDAKPREVLLEERG 360
           IEAKRTSRPTSAHSSRPSSAQSSRSEGPG+QGVE   KPRPKVNPFGDAKPREVLLEERG
Sbjct: 301 IEAKRTSRPTSAHSSRPSSAQSSRSEGPGLQGVETAAKPRPKVNPFGDAKPREVLLEERG 360

Query: 361 KDWRKIDLQLEHRAVDRPETEEEKMLKEEIDVLRKELEEGLSLNTNKESVQESAGEMQDL 420
           KDWRKIDLQLEHRAVDRPETEEEKMLKEEIDVLRKELEEGLSLN NKE VQESAGEMQ+L
Sbjct: 361 KDWRKIDLQLEHRAVDRPETEEEKMLKEEIDVLRKELEEGLSLNANKELVQESAGEMQNL 420

Query: 421 RDRISKKERELETLVLDLDDKIRFGKKATERPGSGAGRAAVFPERP-------------- 480
           RD+I+KKERELE L+LDLDDK+RFGKKATERPGS AGRAAVFPERP              
Sbjct: 421 RDKINKKERELEMLILDLDDKVRFGKKATERPGSAAGRAAVFPERPGSGAGRAAVFPERL 480

Query: 481 PSQGSFDESRSFESIDRPRSRGTGTADVWSRSADDRKPIQGARDRGFLGNRDMERSRDRW 527
           PSQGSFDESRSFESIDRPRSR  GTADVWSRS DDRKPIQ ARDRGFLGNRDMERSRDRW
Sbjct: 481 PSQGSFDESRSFESIDRPRSR--GTADVWSRSTDDRKPIQVARDRGFLGNRDMERSRDRW 531

BLAST of Lag0004659 vs. NCBI nr
Match: XP_023545450.1 (eukaryotic translation initiation factor 4B2-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 907.1 bits (2343), Expect = 6.9e-260
Identity = 478/526 (90.87%), Postives = 499/526 (94.87%), Query Frame = 0

Query: 1   MSKPWGGIGAWAADAERAEAEEREAQAAAAASQAESKNFPSLREAVNTKPKKKKMTLSEF 60
           MSKPWGGIGAWAADAERAE EEREAQAAAAASQAESKNFPSLREAV+TKPKKKKM+LSEF
Sbjct: 1   MSKPWGGIGAWAADAERAEDEEREAQAAAAASQAESKNFPSLREAVSTKPKKKKMSLSEF 60

Query: 61  TMGTLTGPGAGSRQTESQGLTPEEMLRLPTGPKDRSGEEMRSGRLGGGFPSYDRQSMPAG 120
           TMGTLTG GAG R T+SQGLTPEEM+RLPTGPKDRSGEE+RSGRLGGGFP+YDRQ M AG
Sbjct: 61  TMGTLTGHGAGGRLTDSQGLTPEEMIRLPTGPKDRSGEEVRSGRLGGGFPTYDRQGMSAG 120

Query: 121 RFRDRDEGDGSWGGGRRSYGGFDEERRGPPPRASNFDQPSRADEVDNWAMTKKSPTLDSG 180
           RFRDRD+GDGSWGGGRRSYGGFDEERRG  PRASNFDQPSRADEVDNWAMTKKSPTLDSG
Sbjct: 121 RFRDRDDGDGSWGGGRRSYGGFDEERRGSSPRASNFDQPSRADEVDNWAMTKKSPTLDSG 180

Query: 181 RQNRYGSLGGGMGGIGGGGIASRADEVDNWASSKKAIPSRSSTFGSGFRDREANEPDRWT 240
           RQNRYGSLGGG+     GGIASRADEVDNWA  KK IPSRSSTFGSG+  REANEPDRW 
Sbjct: 181 RQNRYGSLGGGV-----GGIASRADEVDNWAREKKPIPSRSSTFGSGY--REANEPDRWG 240

Query: 241 RGRSRDSDTERPRLVLDPPKGNVVTNEATRTNKPSPFGAARPREEVLAEKGLDWKKLDTE 300
           RGRSRDS TERPRLVLDPPKGNVVTNEATRTNKP+PFGAARPREEVLAEKGLDWKKLDTE
Sbjct: 241 RGRSRDSVTERPRLVLDPPKGNVVTNEATRTNKPNPFGAARPREEVLAEKGLDWKKLDTE 300

Query: 301 IEAKRTSRPTSAHSSRPSSAQSSRSEGPGIQGVENVVKPRPKVNPFGDAKPREVLLEERG 360
           IEAK+TSRPTSAHSSRPSSAQS+RSEGPG+QGVENVVKPRPKVNPFGDAKPREVLLEERG
Sbjct: 301 IEAKKTSRPTSAHSSRPSSAQSNRSEGPGLQGVENVVKPRPKVNPFGDAKPREVLLEERG 360

Query: 361 KDWRKIDLQLEHRAVDRPETEEEKMLKEEIDVLRKELEEGLSLNTNKESVQESAGEMQDL 420
           KDWRKIDLQLEHRAVDRPETEEEKMLKEEI+VLRKELEEGL LNTNKESVQESAGEMQ+L
Sbjct: 361 KDWRKIDLQLEHRAVDRPETEEEKMLKEEINVLRKELEEGLHLNTNKESVQESAGEMQNL 420

Query: 421 RDRISKKERELETLVLDLDDKIRFGKKATERPGSGAGRAAVFPERPPSQGSFDESRSFES 480
           RDRI+KKERELETLVLDLDDK+RFGKKATERPGSGAG+AA F ERPPSQGSFDESRSFES
Sbjct: 421 RDRINKKERELETLVLDLDDKVRFGKKATERPGSGAGKAAAFQERPPSQGSFDESRSFES 480

Query: 481 IDRPRSRGTGTADVWSRSADDRKPIQGARDRGFLGNRDMERSRDRW 527
           +DRPRSR  GT DVWSRSADDRKPIQGARD GFLGNR++ERSRDRW
Sbjct: 481 MDRPRSR--GTTDVWSRSADDRKPIQGARDGGFLGNRNIERSRDRW 517

BLAST of Lag0004659 vs. NCBI nr
Match: KAG6598244.1 (Eukaryotic translation initiation factor 4B1, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 906.7 bits (2342), Expect = 9.0e-260
Identity = 477/526 (90.68%), Postives = 499/526 (94.87%), Query Frame = 0

Query: 1   MSKPWGGIGAWAADAERAEAEEREAQAAAAASQAESKNFPSLREAVNTKPKKKKMTLSEF 60
           MSKPWGGIGAWAADAERAE EEREAQAAAAASQAESKNFPSLREAV+TKPKKKKM+LSEF
Sbjct: 107 MSKPWGGIGAWAADAERAEDEEREAQAAAAASQAESKNFPSLREAVSTKPKKKKMSLSEF 166

Query: 61  TMGTLTGPGAGSRQTESQGLTPEEMLRLPTGPKDRSGEEMRSGRLGGGFPSYDRQSMPAG 120
           TMGTLTG GAG R T+SQGLTPEEM+RLPTGPKDRSGEE+RSGRLGGGFP+YDRQ M AG
Sbjct: 167 TMGTLTGHGAGGRLTDSQGLTPEEMIRLPTGPKDRSGEEVRSGRLGGGFPTYDRQGMSAG 226

Query: 121 RFRDRDEGDGSWGGGRRSYGGFDEERRGPPPRASNFDQPSRADEVDNWAMTKKSPTLDSG 180
           R+RDRD+GDGSWGGGRRSYGGFDEERRG  PRASNFDQPSRADEVDNWAMTKKSPTLDSG
Sbjct: 227 RYRDRDDGDGSWGGGRRSYGGFDEERRGSSPRASNFDQPSRADEVDNWAMTKKSPTLDSG 286

Query: 181 RQNRYGSLGGGMGGIGGGGIASRADEVDNWASSKKAIPSRSSTFGSGFRDREANEPDRWT 240
           RQNRYGSLGGG+     GGIASRADEVDNWA  KK IPSRSSTFGSG+  REANEPDRW 
Sbjct: 287 RQNRYGSLGGGV-----GGIASRADEVDNWAREKKPIPSRSSTFGSGY--REANEPDRWG 346

Query: 241 RGRSRDSDTERPRLVLDPPKGNVVTNEATRTNKPSPFGAARPREEVLAEKGLDWKKLDTE 300
           RGRSRDS TERPRLVLDPPKGNVVTNEATRTNKP+PFGAARPREEVLAEKGLDWKKLDTE
Sbjct: 347 RGRSRDSVTERPRLVLDPPKGNVVTNEATRTNKPNPFGAARPREEVLAEKGLDWKKLDTE 406

Query: 301 IEAKRTSRPTSAHSSRPSSAQSSRSEGPGIQGVENVVKPRPKVNPFGDAKPREVLLEERG 360
           IEAK+TSRPTSAHSSRPSSAQSSRSEGPG+QGVENVVKPRPKVNPFGDAKPREVLLEERG
Sbjct: 407 IEAKKTSRPTSAHSSRPSSAQSSRSEGPGLQGVENVVKPRPKVNPFGDAKPREVLLEERG 466

Query: 361 KDWRKIDLQLEHRAVDRPETEEEKMLKEEIDVLRKELEEGLSLNTNKESVQESAGEMQDL 420
           KDWRKIDLQLEHRAVDRPETEEEKMLKEEI+VLRKELEEGL LNTNKESVQESAGEMQ+L
Sbjct: 467 KDWRKIDLQLEHRAVDRPETEEEKMLKEEINVLRKELEEGLHLNTNKESVQESAGEMQNL 526

Query: 421 RDRISKKERELETLVLDLDDKIRFGKKATERPGSGAGRAAVFPERPPSQGSFDESRSFES 480
           RDRI+KKERELETL+LDLDDK+RFGKKATERPGSGAG+AA F ERPPSQGSFDESRSFES
Sbjct: 527 RDRINKKERELETLILDLDDKVRFGKKATERPGSGAGKAAPFQERPPSQGSFDESRSFES 586

Query: 481 IDRPRSRGTGTADVWSRSADDRKPIQGARDRGFLGNRDMERSRDRW 527
           +DRPRSR  GT DVWSRSADDRKPIQGARD GFLGNR++ERSRDRW
Sbjct: 587 VDRPRSR--GTTDVWSRSADDRKPIQGARDGGFLGNRNIERSRDRW 623

BLAST of Lag0004659 vs. NCBI nr
Match: XP_022996764.1 (eukaryotic translation initiation factor 4B2-like [Cucurbita maxima])

HSP 1 Score: 904.4 bits (2336), Expect = 4.5e-259
Identity = 476/526 (90.49%), Postives = 497/526 (94.49%), Query Frame = 0

Query: 1   MSKPWGGIGAWAADAERAEAEEREAQAAAAASQAESKNFPSLREAVNTKPKKKKMTLSEF 60
           MSKPWGGIGAWAADAERAE EEREAQAAAAASQAESKNFPSLREAV+TKPKKKKM+LSEF
Sbjct: 1   MSKPWGGIGAWAADAERAEDEEREAQAAAAASQAESKNFPSLREAVSTKPKKKKMSLSEF 60

Query: 61  TMGTLTGPGAGSRQTESQGLTPEEMLRLPTGPKDRSGEEMRSGRLGGGFPSYDRQSMPAG 120
           TMGTLTG GAG R T+SQGLTPEEM+RLPTGPKDRSGEE+RSGRLGGGFP+YDRQ M AG
Sbjct: 61  TMGTLTGHGAGGRLTDSQGLTPEEMIRLPTGPKDRSGEEVRSGRLGGGFPTYDRQGMSAG 120

Query: 121 RFRDRDEGDGSWGGGRRSYGGFDEERRGPPPRASNFDQPSRADEVDNWAMTKKSPTLDSG 180
           RFRDRD+GDGSWGGGRRSYGGFDEERRG  PRASNFDQPSRADEVDNWAMTK+SPTLDSG
Sbjct: 121 RFRDRDDGDGSWGGGRRSYGGFDEERRGSSPRASNFDQPSRADEVDNWAMTKRSPTLDSG 180

Query: 181 RQNRYGSLGGGMGGIGGGGIASRADEVDNWASSKKAIPSRSSTFGSGFRDREANEPDRWT 240
           RQNRYGSLGGG+     GGIASRADEVDNWA  KK IPSRSSTFGSG+  REANEPDRW 
Sbjct: 181 RQNRYGSLGGGV-----GGIASRADEVDNWAREKKPIPSRSSTFGSGY--REANEPDRWG 240

Query: 241 RGRSRDSDTERPRLVLDPPKGNVVTNEATRTNKPSPFGAARPREEVLAEKGLDWKKLDTE 300
           RGRSRDS TERPRLVLDPPKGNVVTNEATRTNKP+PFGAARPREEVLAEKGLDWKKLDTE
Sbjct: 241 RGRSRDSVTERPRLVLDPPKGNVVTNEATRTNKPNPFGAARPREEVLAEKGLDWKKLDTE 300

Query: 301 IEAKRTSRPTSAHSSRPSSAQSSRSEGPGIQGVENVVKPRPKVNPFGDAKPREVLLEERG 360
           IEAK+TSRPTSAHSSRPSSAQSSRSEGPG+QGVENVVKPRPKVNPFGDAKPREVLLEERG
Sbjct: 301 IEAKKTSRPTSAHSSRPSSAQSSRSEGPGLQGVENVVKPRPKVNPFGDAKPREVLLEERG 360

Query: 361 KDWRKIDLQLEHRAVDRPETEEEKMLKEEIDVLRKELEEGLSLNTNKESVQESAGEMQDL 420
           KDWRKIDLQLEHRAVDRPETEEEKMLKEEI+VLRKELEEGL LNTNKESVQESAGEMQ L
Sbjct: 361 KDWRKIDLQLEHRAVDRPETEEEKMLKEEINVLRKELEEGLHLNTNKESVQESAGEMQKL 420

Query: 421 RDRISKKERELETLVLDLDDKIRFGKKATERPGSGAGRAAVFPERPPSQGSFDESRSFES 480
           RDRI+KKERELE L+LDLDDK+RFGKKATERPGSGAG+AA F ERPPSQGSFDESRSFES
Sbjct: 421 RDRINKKERELEMLILDLDDKVRFGKKATERPGSGAGKAAAFQERPPSQGSFDESRSFES 480

Query: 481 IDRPRSRGTGTADVWSRSADDRKPIQGARDRGFLGNRDMERSRDRW 527
           +DRPRSR  GT DVWSRSADDRKPIQGARD GFLGNR++ERSRDRW
Sbjct: 481 VDRPRSR--GTTDVWSRSADDRKPIQGARDGGFLGNRNIERSRDRW 517

BLAST of Lag0004659 vs. ExPASy Swiss-Prot
Match: Q9SAD7 (Eukaryotic translation initiation factor 4B2 OS=Arabidopsis thaliana OX=3702 GN=EIF4B2 PE=1 SV=1)

HSP 1 Score: 526.2 bits (1354), Expect = 4.3e-148
Identity = 331/566 (58.48%), Postives = 395/566 (69.79%), Query Frame = 0

Query: 1   MSKPWGGIGAWAADAERAEAEEREAQAAAAASQAESKNFPSLREAVNTK--PKKKKMTLS 60
           MSKPWGGIGAWA +AERA+ EE+ A+A A A  A+S++FPSL+EA   K   KKKKMTLS
Sbjct: 1   MSKPWGGIGAWADEAERAD-EEQAAEATATA--ADSQSFPSLKEAATAKSSKKKKKMTLS 60

Query: 61  EFTMGTLTGPGAGSRQTESQGLTPEEMLRLPTGPKDRSGEEMRSGRLGGGFPSY--DRQS 120
           EFT G  T P        S GLT E+ML+LPTGP+ RS +EM+ GRLGGGF SY   R S
Sbjct: 61  EFTKGAYTAP-------SSAGLTREQMLQLPTGPRQRSEDEMQPGRLGGGFSSYGGGRSS 120

Query: 121 MPAGRF-RDRDEGDGSW---GGGRRSYGGFDEERRGPPPRASNFDQPSRADEVDNWAMTK 180
            P GR  RDR++ DGSW   GGGRRSYGGFD+++RG   R S+F Q SRADE D+W   K
Sbjct: 121 GPPGRMSRDREDSDGSWGGGGGGRRSYGGFDDDQRGSNSRVSDFPQVSRADEDDDWGKGK 180

Query: 181 KS-PTLDSGRQ-NRYGSLGGGMGGIGGGGIA-----------------SRADEVDNWASS 240
           KS P+ D GRQ +RYG  GG  GG GGGG                   S+ADEVDNWA+ 
Sbjct: 181 KSLPSFDQGRQGSRYGGGGGSFGGGGGGGAGSYGGGGAGAGSGGGGGFSKADEVDNWAAG 240

Query: 241 KKAIPSRSSTFGSGFRDREANEPDRWTRG---RSRDSDTERPRLVLDPPKGNVVTNE--- 300
           K    ++SSTFGSGFR+    EPDRW RG          ER RLV +P K +   +E   
Sbjct: 241 K----AKSSTFGSGFRE-SGPEPDRWARGVLPSGGGVQEERRRLVFEPRKADTEVSETPT 300

Query: 301 ATRTNKPSPFGAARPREEVLAEKGLDWKKLDTEIEAKR--TSRPTSAHSSRPSSAQSSRS 360
           A +T+KPSPFGAARPRE+VLAEKGLDWKKLD++IEAK+  TSRP+SA SSRPSSAQS+RS
Sbjct: 301 AVKTSKPSPFGAARPREQVLAEKGLDWKKLDSDIEAKKGQTSRPSSAQSSRPSSAQSNRS 360

Query: 361 EGPGIQGVENVVKPRPKVNPFGDAKPREVLLEERGKDWRKIDLQLEHRAVDRPETEEEKM 420
           E   +  VENVVKPRPKVNPFGDAKPREVLLEE+GKDWRKID +LEHR VDRPETE E+M
Sbjct: 361 ESSALNNVENVVKPRPKVNPFGDAKPREVLLEEQGKDWRKIDSELEHRRVDRPETEGERM 420

Query: 421 LKEEIDVLRKELE-EGLSLNTNKESVQESAGEMQDLRDRISKKERELETLVLDLDDKIRF 480
           LKEEI+ LRK+LE E      +KES QES    Q+L D I +KE+ L+ L+ +LDDK+RF
Sbjct: 421 LKEEIEELRKKLEKEAAIAPESKESQQESDSNHQNLPDLIREKEKNLDLLIRELDDKVRF 480

Query: 481 GKKATERPGSGAGRAAVFPERPPSQ-GSFDESRSFESIDRPRSRGTGTADVWSRSADDRK 527
             +A ERPGS A R   + ERP S+ GS DESRS ES++RPRS GTG  D W R  DDR+
Sbjct: 481 RPRAVERPGSSASRGGSYSERPHSRAGSIDESRSVESMERPRSHGTG--DNWPRPVDDRR 540

BLAST of Lag0004659 vs. ExPASy Swiss-Prot
Match: Q9LIN5 (Eukaryotic translation initiation factor 4B1 OS=Arabidopsis thaliana OX=3702 GN=EIF4B1 PE=1 SV=1)

HSP 1 Score: 489.6 bits (1259), Expect = 4.5e-137
Identity = 320/563 (56.84%), Postives = 377/563 (66.96%), Query Frame = 0

Query: 1   MSKPWG--GIGAWAADAERAEAEEREAQAAAAASQAESKNFPSLREAV------NTKPKK 60
           MSK WG  GIGAWA +AERA+ EE+ A+A AA   A++++FPSLREA           K 
Sbjct: 1   MSKAWGGIGIGAWADEAERAD-EEQAAEATAAT--ADTQSFPSLREAAAATATSGKSRKM 60

Query: 61  KKMTLSEFTMGTLTGPGAGSRQTESQGLTPEEMLRLPTGPKDRSGEEMRSGRLGGGFPSY 120
           KKM+LSEFT G  T PG       S GLT +E+L+LPTGP+ RS EEM+ GRLGGGF SY
Sbjct: 61  KKMSLSEFTTGAYTAPGG----RNSVGLTQQEILQLPTGPRQRSEEEMQPGRLGGGFSSY 120

Query: 121 DRQSMPAGRF-RDRDEGDGSW-----GGGRRSY-GGFDEERRGPPPRASNFDQPSRADEV 180
             +S   GR  RDRD+ DGSW     GGGRR Y GGFD++RRG   R S+F QPSRADEV
Sbjct: 121 GGRS--GGRIGRDRDDSDGSWSGGGGGGGRRPYGGGFDDDRRGNQSRVSDFPQPSRADEV 180

Query: 181 DNWAMTKKS-PTLDSGRQNRYGSLGGGM---------GGIGGGGIASRADEVDNWASSKK 240
           D+W   KK  P+ D GRQ RY   GGG          GG GGGG  SRAD+VDNW + K+
Sbjct: 181 DDWGKEKKPLPSFDQGRQGRYSGDGGGFGGGGSGFGGGGGGGGGGLSRADDVDNWGAGKR 240

Query: 241 AIPSRSSTFGSGFRDREANEPDRWTRGRSRDSDTERPRLVLDPPK----GNVVTNEATRT 300
             P RSSTFGS F D                   ER RLVL+P K    G+       +T
Sbjct: 241 QAPVRSSTFGSSFGD---------------SGQEERRRLVLEPRKVESGGSETPPVVEKT 300

Query: 301 NKPSPFGAARPREEVLAEKGLDWKKLDTEIEAKR----TSRPTSAHSSRPSSAQSSRSEG 360
           +KP+PFGAARPRE+VLAEKGLDWKK+D+EIEAK+    TSRPTSAHSSRPSSAQS+RSE 
Sbjct: 301 SKPNPFGAARPREDVLAEKGLDWKKIDSEIEAKKGSSQTSRPTSAHSSRPSSAQSNRSES 360

Query: 361 PGIQGVENVVKPRPKVNPFGDAKPREVLLEERGKDWRKIDLQLEHRAVDRPETEEEKMLK 420
               G+ NVVKPRPKVNPFGDAKPREVLLEE+GKDWRK+DL+LEHR VDRPETEEEKMLK
Sbjct: 361 ---SGLNNVVKPRPKVNPFGDAKPREVLLEEQGKDWRKMDLELEHRRVDRPETEEEKMLK 420

Query: 421 EEIDVLRKELEEGLSLNTNKESVQE--SAGEMQDLRDRISKKERELETLVLDLDDKIRFG 480
           EEI+ LRK+LE+       KES QE  S     DL + I  KE++LE L  +LDDK+RF 
Sbjct: 421 EEIEELRKKLEKESVAPEIKESDQEPGSNNNHNDLPEIIRGKEKDLEILTRELDDKVRFR 480

Query: 481 KKATERPGSGAGRAAVFPERPPSQ-GSFDESRSFESIDRPRSRGTGTADVWSRSADD-RK 527
           +K  ERPGSGAGR   + ER  S+ GS DE+RSFES +RPRSR  G  D W R A++ R+
Sbjct: 481 QKPVERPGSGAGRTGTYSERTHSRAGSIDETRSFESTERPRSR--GAVDAWVRPANEQRR 532

BLAST of Lag0004659 vs. ExPASy Swiss-Prot
Match: Q9AUJ7 (Eukaryotic translation initiation factor 4B1 OS=Triticum aestivum OX=4565 GN=EIF4B PE=1 SV=1)

HSP 1 Score: 348.2 bits (892), Expect = 1.6e-94
Identity = 259/585 (44.27%), Postives = 344/585 (58.80%), Query Frame = 0

Query: 1   MSKPWGGIGAWAADAERAEAEEREAQAA-------AAASQAESKNFPSLREAV---NTKP 60
           M+KPWGG+GAWA DAER E EERE  AA       AAA  A S  FPSL+EAV     K 
Sbjct: 1   MAKPWGGVGAWALDAER-EDEEREHAAAFPAPDPPAAAGGAAS--FPSLKEAVVAGGGKQ 60

Query: 61  KKKK---MTLSEFTMGTLTGPGAGSRQTESQGLTPEEMLRLPTGPKDRSGEEMRSGRLGG 120
           KKKK   ++LSEFT     G        E +GLTP+EM+ LPTGP++RS +E+       
Sbjct: 61  KKKKGTTLSLSEFTTYGAAGAPRRVAPAEPKGLTPQEMMMLPTGPRERSEDEL------- 120

Query: 121 GFPSYDRQSMPAGRFRDRDEGDGSWGGGRRSY-GGFDEERRGPPPRASNFDQPSRADEVD 180
                           DR  G  S+GG R    GGFD++RR    R S+ D PSRADE D
Sbjct: 121 ----------------DRSRGFRSYGGDREPRGGGFDDDRRS--SRDSDLDMPSRADESD 180

Query: 181 NWAMTKK---SPTLDSGRQNRYGSLGGGMGGIGGGGIASRADEVDNWASSKKAIPSRSST 240
           NW   K    +PT DSGR++R          + G     R+D++DNW+  KK +PSR  +
Sbjct: 181 NWGKNKSFSPAPT-DSGRRDR----------LSGPSPLGRSDDIDNWSRDKKPLPSRYPS 240

Query: 241 FGS--------------------------GFRDREA-NEPDRWTRG---RSRDSDTERPR 300
            G+                          GFRD    ++ DRW RG       ++ +RPR
Sbjct: 241 LGTGGGFRESSGGGFRESSGGGFRESSGGGFRDSPGPSDSDRWVRGAVPAPMTNNGDRPR 300

Query: 301 LVLDPPKGN-----VVTNEATRTNKPSPFGAARPREEVLAEKGLDWKKLDTEIEAKRTSR 360
           L L+PPK +     V   E  R ++PSPFGAA+PREEVLAEKGLDW+K++ EIE K+TSR
Sbjct: 301 LNLNPPKRDPSATPVPAAEVAR-SRPSPFGAAKPREEVLAEKGLDWRKMEGEIE-KKTSR 360

Query: 361 PTSAHSSRPSSAQSSRSEGPGIQ----GVENVVKPRPKVNPFGDAKPREVLLEERGKDWR 420
           PTS+HSSRP+SA SSR   PG Q    G E   + RPKVNPFGDAKPREV+L+E+GKDWR
Sbjct: 361 PTSSHSSRPNSAHSSRPGSPGSQVSAVGSEGAPRARPKVNPFGDAKPREVVLQEKGKDWR 420

Query: 421 KIDLQLEHRAVDRPETEEEKMLKEEIDVLRKELEEGLSLNTNKESVQESAGEMQDLRDRI 480
           KIDL+LEHRAV+RPE+EEEK LKEEI++L+ +L+E  ++        + + + +++ ++I
Sbjct: 421 KIDLELEHRAVNRPESEEEKNLKEEINLLKVDLKEIEAI------AGDGSEQAKEVSEKI 480

Query: 481 SKKERELETLVLDLDDKIRFGKKATERPGSGAGRAAVFPERPPSQGSFDESRSFESIDRP 527
           S+ E++L+ L ++LDDKIRFG    +RP SGAGRAA FP  P S+   +   +   +DRP
Sbjct: 481 SQMEKQLDLLTVELDDKIRFG----QRPSSGAGRAAAFP--PASE---EPHVAVAHMDRP 527

BLAST of Lag0004659 vs. ExPASy Swiss-Prot
Match: Q9SZP8 (Eukaryotic translation initiation factor 4B3 OS=Arabidopsis thaliana OX=3702 GN=EIF4B3 PE=1 SV=1)

HSP 1 Score: 139.0 bits (349), Expect = 1.5e-31
Identity = 162/477 (33.96%), Postives = 226/477 (47.38%), Query Frame = 0

Query: 1   MSKPWGGIGAWAADAERAEAE--EREAQAAAAASQAESKNFPSLREAVNTKPKKKK---M 60
           +S  W   GAWA +AE  EAE  ++ +     +S  +S +FPSL  A  TK KKKK   +
Sbjct: 5   VSSVWAKPGAWALEAEEHEAELKQQPSPTNQKSSAEDSSDFPSLAAAATTKTKKKKGQTI 64

Query: 61  TLSEF-TMGTLTGPGAGSRQTESQGLTPEEMLRLPTGPKDRSGEEMRSGRLGGGFPSYDR 120
           +L+EF T GT     A     +++ LT  E++ LPTGP++RS EE+   +LGGGF SY  
Sbjct: 65  SLAEFATYGTAKAKPA----PQTERLTQAELVALPTGPRERSAEELDRSKLGGGFRSYG- 124

Query: 121 QSMPAGRFRDRDEGDGSWGG------GRRSYGGFDEERRGPPPRASNFDQPSRADEVDNW 180
                GR+ D +     WG       G R  GGF+ +R   P R S    PSRADE DNW
Sbjct: 125 ----GGRYGD-ESSSSRWGSSRVSEDGERRGGGFNRDRE--PSRDSG---PSRADEDDNW 184

Query: 181 AMTKKSPTLDSGRQNRYGSLGGGMGGIGGGGIASRADEVDNWASSKKAIPSR--SSTFGS 240
           A  KK P   +G + R    G   GG       S+ADEVD+W S+K + P R  SS  G 
Sbjct: 185 AAAKK-PISGNGFERRERGSG---GGFFESQSQSKADEVDSWVSTKPSEPRRFVSSNGGG 244

Query: 241 G------------FRDREA-------NEPDRWTRGRSRD---------SDTERPRLVLDP 300
           G             R+R++       +E D W R R            S   RPRLVL P
Sbjct: 245 GDRFEKRGSFESLSRNRDSQYGGGGGSESDTWGRRREESGAANGSPPPSGGSRPRLVLQP 304

Query: 301 PKGNVVTNEATRTNKP-----------SPFGAARPREEVLAEKGLDWKKLDTEIEAKRTS 360
               V   E  +   P           +PFG ARPREEVLAEKG DWK++D ++EA++  
Sbjct: 305 RTLPVAVVEVVKPESPVLVIVEKPKGANPFGNARPREEVLAEKGQDWKEIDEKLEAEKL- 364

Query: 361 RPTSAHSSRPSSAQSSRSEGPGIQGVENVVKPRPKVNPFGDAKPREVLLEERGKDWRKID 420
           +  +A   +P    + +S G    G+ N  K   ++      K  E   E+  ++   ++
Sbjct: 365 KDIAAAMEKP----NEKSTGKMGFGLGNGRKDEERIER-SWRKSTEHSEEDAQEEEPAVE 424

Query: 421 LQLEHRAVDRPETEEEKMLKEEIDVLRKELEEGLSLNTNKESVQESAGEMQDLRDRI 425
              +    D+P  EE K  KEE +   + +EE     T  E   E A + ++  D+I
Sbjct: 425 GAKKEETEDKPAVEEAK--KEETEG-EQAVEEAKKEETGGEPAVEEA-KKEETEDKI 452

BLAST of Lag0004659 vs. ExPASy TrEMBL
Match: A0A0A0LKJ9 (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_2G349620 PE=4 SV=1)

HSP 1 Score: 916.8 bits (2368), Expect = 4.2e-263
Identity = 486/540 (90.00%), Postives = 502/540 (92.96%), Query Frame = 0

Query: 1   MSKPWGGIGAWAADAERAEAEEREAQAAAAASQAESKNFPSLREAVNTKPKKKKMTLSEF 60
           MSKPWGGIGAWAADAERAEAEEREAQAAAAASQ+ESKNFPSLREAV+TKPKKKKM+LSEF
Sbjct: 1   MSKPWGGIGAWAADAERAEAEEREAQAAAAASQSESKNFPSLREAVSTKPKKKKMSLSEF 60

Query: 61  TMGTLTGPGAGSRQTESQGLTPEEMLRLPTGPKDRSGEEMRSGRLGGGFPSYDRQSMPAG 120
           TMGTLTGPG G R T++QGLTPEEM+RLPTGPKDRSGEE+RSGRLGGGFP+YDRQ M AG
Sbjct: 61  TMGTLTGPGTGGRLTDTQGLTPEEMIRLPTGPKDRSGEEVRSGRLGGGFPTYDRQGMSAG 120

Query: 121 RFRDRDEGDGSWGGGRRSYGGFDEERRGPPPRASNFDQPSRADEVDNWAMTKKSPTLDSG 180
           RFRDRDEGDGSWGGGRRSYGGFDEERRGP  RASNFDQPSRADEVDNWAMTKKSPTLDSG
Sbjct: 121 RFRDRDEGDGSWGGGRRSYGGFDEERRGPSSRASNFDQPSRADEVDNWAMTKKSPTLDSG 180

Query: 181 RQNRYGSLGGGMGGIGGGGIASRADEVDNWASSKKAIPSRSSTFGSGFRDREANEPDRWT 240
           RQNRYGSLGGG+     GGIASRADEVDNWAS KK IPSRSSTFGSG+  REANEPDRW 
Sbjct: 181 RQNRYGSLGGGV-----GGIASRADEVDNWASVKKPIPSRSSTFGSGY--REANEPDRWG 240

Query: 241 RGRSRDSDTERPRLVLDPPKGNVVTNEATRTNKPSPFGAARPREEVLAEKGLDWKKLDTE 300
           RGRSRDSDTERPRLVLDPPKGNV TNEATRTNKPSPFGAARPREEVLAEKGLDWKKLDTE
Sbjct: 241 RGRSRDSDTERPRLVLDPPKGNVATNEATRTNKPSPFGAARPREEVLAEKGLDWKKLDTE 300

Query: 301 IEAKRTSRPTSAHSSRPSSAQSSRSEGPGIQGVENVVKPRPKVNPFGDAKPREVLLEERG 360
           IEAKRTSRPTSAHSSRPSSAQSSRSEGPG+QG+ENV KPRPKVNPFGDAKPREVLLEERG
Sbjct: 301 IEAKRTSRPTSAHSSRPSSAQSSRSEGPGLQGLENVAKPRPKVNPFGDAKPREVLLEERG 360

Query: 361 KDWRKIDLQLEHRAVDRPETEEEKMLKEEIDVLRKELEEGLSLNTNKESVQESAGEMQDL 420
           KDWRKIDLQLEHRAVDRPETEEEKMLKEEIDVLRKELEEGLSLN NKESVQESAGEMQ+L
Sbjct: 361 KDWRKIDLQLEHRAVDRPETEEEKMLKEEIDVLRKELEEGLSLNANKESVQESAGEMQNL 420

Query: 421 RDRISKKERELETLVLDLDDKIRFGKKATERPGSGAGRAA--------------VFPERP 480
           RDRI+KKE ELETLVLDLDDK+RFGKKATERPGSGAGRAA              VFPERP
Sbjct: 421 RDRINKKEHELETLVLDLDDKVRFGKKATERPGSGAGRAAVFPERPGSGAGRATVFPERP 480

Query: 481 PSQGSFDESRSFESIDRPRSRGTGTADVWSRSADDRKPIQGARDRGFLGNRDMERSRDRW 527
           PSQGSFDESRSFES+DRPRSR  GTADVWSR ADDRKPIQGARDRGFLGNRDMERSRDRW
Sbjct: 481 PSQGSFDESRSFESVDRPRSR--GTADVWSRPADDRKPIQGARDRGFLGNRDMERSRDRW 531

BLAST of Lag0004659 vs. ExPASy TrEMBL
Match: A0A6J1K9L6 (eukaryotic translation initiation factor 4B2-like OS=Cucurbita maxima OX=3661 GN=LOC111491898 PE=4 SV=1)

HSP 1 Score: 904.4 bits (2336), Expect = 2.2e-259
Identity = 476/526 (90.49%), Postives = 497/526 (94.49%), Query Frame = 0

Query: 1   MSKPWGGIGAWAADAERAEAEEREAQAAAAASQAESKNFPSLREAVNTKPKKKKMTLSEF 60
           MSKPWGGIGAWAADAERAE EEREAQAAAAASQAESKNFPSLREAV+TKPKKKKM+LSEF
Sbjct: 1   MSKPWGGIGAWAADAERAEDEEREAQAAAAASQAESKNFPSLREAVSTKPKKKKMSLSEF 60

Query: 61  TMGTLTGPGAGSRQTESQGLTPEEMLRLPTGPKDRSGEEMRSGRLGGGFPSYDRQSMPAG 120
           TMGTLTG GAG R T+SQGLTPEEM+RLPTGPKDRSGEE+RSGRLGGGFP+YDRQ M AG
Sbjct: 61  TMGTLTGHGAGGRLTDSQGLTPEEMIRLPTGPKDRSGEEVRSGRLGGGFPTYDRQGMSAG 120

Query: 121 RFRDRDEGDGSWGGGRRSYGGFDEERRGPPPRASNFDQPSRADEVDNWAMTKKSPTLDSG 180
           RFRDRD+GDGSWGGGRRSYGGFDEERRG  PRASNFDQPSRADEVDNWAMTK+SPTLDSG
Sbjct: 121 RFRDRDDGDGSWGGGRRSYGGFDEERRGSSPRASNFDQPSRADEVDNWAMTKRSPTLDSG 180

Query: 181 RQNRYGSLGGGMGGIGGGGIASRADEVDNWASSKKAIPSRSSTFGSGFRDREANEPDRWT 240
           RQNRYGSLGGG+     GGIASRADEVDNWA  KK IPSRSSTFGSG+  REANEPDRW 
Sbjct: 181 RQNRYGSLGGGV-----GGIASRADEVDNWAREKKPIPSRSSTFGSGY--REANEPDRWG 240

Query: 241 RGRSRDSDTERPRLVLDPPKGNVVTNEATRTNKPSPFGAARPREEVLAEKGLDWKKLDTE 300
           RGRSRDS TERPRLVLDPPKGNVVTNEATRTNKP+PFGAARPREEVLAEKGLDWKKLDTE
Sbjct: 241 RGRSRDSVTERPRLVLDPPKGNVVTNEATRTNKPNPFGAARPREEVLAEKGLDWKKLDTE 300

Query: 301 IEAKRTSRPTSAHSSRPSSAQSSRSEGPGIQGVENVVKPRPKVNPFGDAKPREVLLEERG 360
           IEAK+TSRPTSAHSSRPSSAQSSRSEGPG+QGVENVVKPRPKVNPFGDAKPREVLLEERG
Sbjct: 301 IEAKKTSRPTSAHSSRPSSAQSSRSEGPGLQGVENVVKPRPKVNPFGDAKPREVLLEERG 360

Query: 361 KDWRKIDLQLEHRAVDRPETEEEKMLKEEIDVLRKELEEGLSLNTNKESVQESAGEMQDL 420
           KDWRKIDLQLEHRAVDRPETEEEKMLKEEI+VLRKELEEGL LNTNKESVQESAGEMQ L
Sbjct: 361 KDWRKIDLQLEHRAVDRPETEEEKMLKEEINVLRKELEEGLHLNTNKESVQESAGEMQKL 420

Query: 421 RDRISKKERELETLVLDLDDKIRFGKKATERPGSGAGRAAVFPERPPSQGSFDESRSFES 480
           RDRI+KKERELE L+LDLDDK+RFGKKATERPGSGAG+AA F ERPPSQGSFDESRSFES
Sbjct: 421 RDRINKKERELEMLILDLDDKVRFGKKATERPGSGAGKAAAFQERPPSQGSFDESRSFES 480

Query: 481 IDRPRSRGTGTADVWSRSADDRKPIQGARDRGFLGNRDMERSRDRW 527
           +DRPRSR  GT DVWSRSADDRKPIQGARD GFLGNR++ERSRDRW
Sbjct: 481 VDRPRSR--GTTDVWSRSADDRKPIQGARDGGFLGNRNIERSRDRW 517

BLAST of Lag0004659 vs. ExPASy TrEMBL
Match: A0A1S3BA92 (eukaryotic translation initiation factor 4B2 OS=Cucumis melo OX=3656 GN=LOC103487724 PE=4 SV=1)

HSP 1 Score: 902.1 bits (2330), Expect = 1.1e-258
Identity = 486/572 (84.97%), Postives = 504/572 (88.11%), Query Frame = 0

Query: 1   MSKPWGGIGAWAADAERAEAEEREAQ----AAAAASQAESKNFPSLREAVNTKPKKKKMT 60
           MSKPWGGIGAWAADAERAEAEEREAQ    AAAAASQAESKNFPSLREAV+TKPKKKKM+
Sbjct: 1   MSKPWGGIGAWAADAERAEAEEREAQAAAAAAAAASQAESKNFPSLREAVSTKPKKKKMS 60

Query: 61  LSEFTMGTLTGPGAGSRQTESQGLTPEEMLRLPTGPKDRSGEEMRSGRLGGGFPSYDRQS 120
           LSEFTMGTLTGPG G R T+SQGLTPEEM+RLPTGPKDRSGEE+RSGRLGGGFP+YDRQS
Sbjct: 61  LSEFTMGTLTGPGTGGRLTDSQGLTPEEMIRLPTGPKDRSGEEVRSGRLGGGFPTYDRQS 120

Query: 121 MPAGRFRDRDEGDGSWGGGRRSYGGFDEERRGPPPRASNFDQPSRADEVDNWAMTKKSPT 180
           M AGRFRDRDEGDGSWGGGRRSYGGFDEERRGP PRASNFDQPSRADEVDNWAMTKKSPT
Sbjct: 121 MSAGRFRDRDEGDGSWGGGRRSYGGFDEERRGPSPRASNFDQPSRADEVDNWAMTKKSPT 180

Query: 181 LDSGRQNRYGSLGGGMGGIGGGGIASRADEVDNWASSKKAIPSRSSTFGSGFRDREANEP 240
           LDSGRQNRYGSLGGGM     GG+ASRADEVDNWAS KK IPSRSSTFGSG+  REANEP
Sbjct: 181 LDSGRQNRYGSLGGGM-----GGMASRADEVDNWASVKKPIPSRSSTFGSGY--REANEP 240

Query: 241 DRWTRGRSRDSDTERPRLVLDPPKGNVVTNEATRTNKPSPFGAARPREEVLAEKGLDWKK 300
           DRW RGRSRDSDTERPRLVLDPPKGNVVTNEATRTNKP+PFGAARPREEVLAEKGLDWKK
Sbjct: 241 DRWGRGRSRDSDTERPRLVLDPPKGNVVTNEATRTNKPNPFGAARPREEVLAEKGLDWKK 300

Query: 301 LDTEIEAKRTSRPTSAHSSRPSSAQSSRSEGPGIQGVENVVKPRPKVNPFGDAKPREVLL 360
           LDTEIEAKRTSRPTSAHSSRPSSAQSSRSEGPG+QGVEN+ KPRPKVNPFGDAKPREVLL
Sbjct: 301 LDTEIEAKRTSRPTSAHSSRPSSAQSSRSEGPGLQGVENMAKPRPKVNPFGDAKPREVLL 360

Query: 361 EERGKDWRKIDLQLEHRAVDRPETEEEKMLKEEIDVLRKELEEGLSLNTNKESVQESAGE 420
           EERGKDWRKIDLQLEHRAVDRPETEEEKMLKEEIDVLRKELEEGLSLN NKESVQESAGE
Sbjct: 361 EERGKDWRKIDLQLEHRAVDRPETEEEKMLKEEIDVLRKELEEGLSLNANKESVQESAGE 420

Query: 421 MQDLRDRISKKERELETLVLDLDDKIRFGKKATERPGSGAGRAA---------------- 480
           MQ+LR+RI+KKE ELETL+LDLDDK+RFGKKAT+RPGSGAGRAA                
Sbjct: 421 MQNLRERINKKEHELETLILDLDDKVRFGKKATDRPGSGAGRAAVFPERPGSGAGRAAVF 480

Query: 481 --------------------------VFPERPPSQGSFDESRSFESIDRPRSRGTGTADV 527
                                     VFPERPPSQGSFDESRSFES+DRPRSR  GTADV
Sbjct: 481 PERPGSGAGRAAVFPERPGSGAGRATVFPERPPSQGSFDESRSFESVDRPRSR--GTADV 540

BLAST of Lag0004659 vs. ExPASy TrEMBL
Match: A0A6J1HBJ6 (eukaryotic translation initiation factor 4B2-like OS=Cucurbita moschata OX=3662 GN=LOC111462515 PE=4 SV=1)

HSP 1 Score: 901.4 bits (2328), Expect = 1.8e-258
Identity = 474/526 (90.11%), Postives = 496/526 (94.30%), Query Frame = 0

Query: 1   MSKPWGGIGAWAADAERAEAEEREAQAAAAASQAESKNFPSLREAVNTKPKKKKMTLSEF 60
           MSKPWGGIGAWAADAERAE EEREAQAAAAASQAESKNFPSLREAV+TKPKKKKM+LSEF
Sbjct: 1   MSKPWGGIGAWAADAERAEDEEREAQAAAAASQAESKNFPSLREAVSTKPKKKKMSLSEF 60

Query: 61  TMGTLTGPGAGSRQTESQGLTPEEMLRLPTGPKDRSGEEMRSGRLGGGFPSYDRQSMPAG 120
           TMGTLTG GAG R T+SQGLTPEEM+RLPTGPKDRSGEE+RSGRLGGGFP+YDRQ M AG
Sbjct: 61  TMGTLTGHGAGGRLTDSQGLTPEEMIRLPTGPKDRSGEEVRSGRLGGGFPTYDRQGMSAG 120

Query: 121 RFRDRDEGDGSWGGGRRSYGGFDEERRGPPPRASNFDQPSRADEVDNWAMTKKSPTLDSG 180
           R+RDRD+GDGSWGGGRRSYGGFDEERRG  PRASNFDQPSRADEVDNWAMTKKSPTLDSG
Sbjct: 121 RYRDRDDGDGSWGGGRRSYGGFDEERRGSSPRASNFDQPSRADEVDNWAMTKKSPTLDSG 180

Query: 181 RQNRYGSLGGGMGGIGGGGIASRADEVDNWASSKKAIPSRSSTFGSGFRDREANEPDRWT 240
           RQNRYGSLGGG+     GGIASRADEVDNWA  KK IPSRSSTFGS +  REANEPDRW 
Sbjct: 181 RQNRYGSLGGGV-----GGIASRADEVDNWAREKKPIPSRSSTFGSSY--REANEPDRWG 240

Query: 241 RGRSRDSDTERPRLVLDPPKGNVVTNEATRTNKPSPFGAARPREEVLAEKGLDWKKLDTE 300
           RGRSRDS TERPRLVLDPPKGNVVTNEA R NKP+PFGAARPREEVLAEKGLDWKKLDTE
Sbjct: 241 RGRSRDSVTERPRLVLDPPKGNVVTNEAARANKPNPFGAARPREEVLAEKGLDWKKLDTE 300

Query: 301 IEAKRTSRPTSAHSSRPSSAQSSRSEGPGIQGVENVVKPRPKVNPFGDAKPREVLLEERG 360
           IEAK+TSRPTSAHSSRPSSAQSSRSEGPG+QGVENVVKPRPKVNPFGDAKPREVLLEERG
Sbjct: 301 IEAKKTSRPTSAHSSRPSSAQSSRSEGPGLQGVENVVKPRPKVNPFGDAKPREVLLEERG 360

Query: 361 KDWRKIDLQLEHRAVDRPETEEEKMLKEEIDVLRKELEEGLSLNTNKESVQESAGEMQDL 420
           KDWRKIDLQLEHRAVDRPETEEEKMLKEEI+VLRKELEEGL LNTNKESVQESAGEMQ+L
Sbjct: 361 KDWRKIDLQLEHRAVDRPETEEEKMLKEEINVLRKELEEGLHLNTNKESVQESAGEMQNL 420

Query: 421 RDRISKKERELETLVLDLDDKIRFGKKATERPGSGAGRAAVFPERPPSQGSFDESRSFES 480
           RDRI+KKERELETL+LDLDDK+RFGKKATERPGSGAG+AA F ERPPSQGSFDESRSFES
Sbjct: 421 RDRINKKERELETLILDLDDKVRFGKKATERPGSGAGKAAAFQERPPSQGSFDESRSFES 480

Query: 481 IDRPRSRGTGTADVWSRSADDRKPIQGARDRGFLGNRDMERSRDRW 527
           +DRPRSR  GT DVWSRSADDRKPIQGARD GFLGNR++ERSRDRW
Sbjct: 481 VDRPRSR--GTTDVWSRSADDRKPIQGARDGGFLGNRNIERSRDRW 517

BLAST of Lag0004659 vs. ExPASy TrEMBL
Match: A0A6J1KMX7 (eukaryotic translation initiation factor 4B2-like OS=Cucurbita maxima OX=3661 GN=LOC111496056 PE=4 SV=1)

HSP 1 Score: 893.6 bits (2308), Expect = 3.8e-256
Identity = 470/526 (89.35%), Postives = 495/526 (94.11%), Query Frame = 0

Query: 1   MSKPWGGIGAWAADAERAEAEEREAQAAAAASQAESKNFPSLREAVNTKPKKKKMTLSEF 60
           MSKPWGGIGAWAADAERAEAEEREAQAAAAASQAESKNFPSL+EAVNTKPKKKKMTLSEF
Sbjct: 1   MSKPWGGIGAWAADAERAEAEEREAQAAAAASQAESKNFPSLKEAVNTKPKKKKMTLSEF 60

Query: 61  TMGTLTGPGAGSRQTESQGLTPEEMLRLPTGPKDRSGEEMRSGRLGGGFPSYDRQSMPAG 120
           TMGTLTGPGAGSRQT+S+GLTPEEMLRLPTGPKDRSGEEMR GRLGGGFP+YDR SMPAG
Sbjct: 61  TMGTLTGPGAGSRQTDSKGLTPEEMLRLPTGPKDRSGEEMRPGRLGGGFPTYDRHSMPAG 120

Query: 121 RFRDRDEGDGSWGGGRRSYGGFDEERRGPPPRASNFDQPSRADEVDNWAMTKKSPTLDSG 180
           RFRDRDEGDGSWGGGRRS+GGFD++RR PPPRASNFDQPSRADEVDNWAMTKKSPTL S 
Sbjct: 121 RFRDRDEGDGSWGGGRRSFGGFDDDRRDPPPRASNFDQPSRADEVDNWAMTKKSPTLASE 180

Query: 181 RQNRYGSLGGGMGGIGGGGIASRADEVDNWASSKKAIPSRSSTFGSGFRDREANEPDRWT 240
           RQNRYGSLGGGM     GGIASRADEVD+WAS+KK +PSRSSTFGSG+RDRE NE DRWT
Sbjct: 181 RQNRYGSLGGGM-----GGIASRADEVDSWASAKKPVPSRSSTFGSGYRDREVNEHDRWT 240

Query: 241 RGRSRDSDTERPRLVLDPPKGNVVTNEATRTNKPSPFGAARPREEVLAEKGLDWKKLDTE 300
           RG SRDSDTERPRLVLD PK N++TNEATR+NKPSPFGAARPREEVLAEKGLDWKKLDTE
Sbjct: 241 RGSSRDSDTERPRLVLDRPKANLLTNEATRSNKPSPFGAARPREEVLAEKGLDWKKLDTE 300

Query: 301 IEAKRTSRPTSAHSSRPSSAQSSRSEGPGIQGVENVVKPRPKVNPFGDAKPREVLLEERG 360
           IEAKRTSRPTSAHSSRPSSAQSSRSEGPG+QGVENVVKPRPKVNPFGDAKPREVLLEERG
Sbjct: 301 IEAKRTSRPTSAHSSRPSSAQSSRSEGPGLQGVENVVKPRPKVNPFGDAKPREVLLEERG 360

Query: 361 KDWRKIDLQLEHRAVDRPETEEEKMLKEEIDVLRKELEEGLSLNTNKESVQESAGEMQDL 420
           KDWRKIDLQLE RAVDRPETEEEKMLKEEIDVLRKELE+GLSLN  KES+QESA E+Q+L
Sbjct: 361 KDWRKIDLQLERRAVDRPETEEEKMLKEEIDVLRKELEKGLSLNAKKESMQESAVEIQNL 420

Query: 421 RDRISKKERELETLVLDLDDKIRFGKKATERPGSGAGRAAVFPERPPSQGSFDESRSFES 480
           RDRI+KKE ELE LVLDLDDK+RFGKKA ERPGSGAGRAAVFPER  SQGSFDESRSFES
Sbjct: 421 RDRINKKECELEALVLDLDDKVRFGKKAAERPGSGAGRAAVFPERQSSQGSFDESRSFES 480

Query: 481 IDRPRSRGTGTADVWSRSADDRKPIQGARDRGFLGNRDMERSRDRW 527
           ++RPRSR  GT DVWSRSA+D KPIQGARD GFLG+RDM RSRDRW
Sbjct: 481 MERPRSR--GTPDVWSRSAEDGKPIQGARDGGFLGSRDMNRSRDRW 519

BLAST of Lag0004659 vs. TAIR 10
Match: AT1G13020.1 (eukaryotic initiation factor 4B2 )

HSP 1 Score: 526.2 bits (1354), Expect = 3.1e-149
Identity = 331/566 (58.48%), Postives = 395/566 (69.79%), Query Frame = 0

Query: 1   MSKPWGGIGAWAADAERAEAEEREAQAAAAASQAESKNFPSLREAVNTK--PKKKKMTLS 60
           MSKPWGGIGAWA +AERA+ EE+ A+A A A  A+S++FPSL+EA   K   KKKKMTLS
Sbjct: 1   MSKPWGGIGAWADEAERAD-EEQAAEATATA--ADSQSFPSLKEAATAKSSKKKKKMTLS 60

Query: 61  EFTMGTLTGPGAGSRQTESQGLTPEEMLRLPTGPKDRSGEEMRSGRLGGGFPSY--DRQS 120
           EFT G  T P        S GLT E+ML+LPTGP+ RS +EM+ GRLGGGF SY   R S
Sbjct: 61  EFTKGAYTAP-------SSAGLTREQMLQLPTGPRQRSEDEMQPGRLGGGFSSYGGGRSS 120

Query: 121 MPAGRF-RDRDEGDGSW---GGGRRSYGGFDEERRGPPPRASNFDQPSRADEVDNWAMTK 180
            P GR  RDR++ DGSW   GGGRRSYGGFD+++RG   R S+F Q SRADE D+W   K
Sbjct: 121 GPPGRMSRDREDSDGSWGGGGGGRRSYGGFDDDQRGSNSRVSDFPQVSRADEDDDWGKGK 180

Query: 181 KS-PTLDSGRQ-NRYGSLGGGMGGIGGGGIA-----------------SRADEVDNWASS 240
           KS P+ D GRQ +RYG  GG  GG GGGG                   S+ADEVDNWA+ 
Sbjct: 181 KSLPSFDQGRQGSRYGGGGGSFGGGGGGGAGSYGGGGAGAGSGGGGGFSKADEVDNWAAG 240

Query: 241 KKAIPSRSSTFGSGFRDREANEPDRWTRG---RSRDSDTERPRLVLDPPKGNVVTNE--- 300
           K    ++SSTFGSGFR+    EPDRW RG          ER RLV +P K +   +E   
Sbjct: 241 K----AKSSTFGSGFRE-SGPEPDRWARGVLPSGGGVQEERRRLVFEPRKADTEVSETPT 300

Query: 301 ATRTNKPSPFGAARPREEVLAEKGLDWKKLDTEIEAKR--TSRPTSAHSSRPSSAQSSRS 360
           A +T+KPSPFGAARPRE+VLAEKGLDWKKLD++IEAK+  TSRP+SA SSRPSSAQS+RS
Sbjct: 301 AVKTSKPSPFGAARPREQVLAEKGLDWKKLDSDIEAKKGQTSRPSSAQSSRPSSAQSNRS 360

Query: 361 EGPGIQGVENVVKPRPKVNPFGDAKPREVLLEERGKDWRKIDLQLEHRAVDRPETEEEKM 420
           E   +  VENVVKPRPKVNPFGDAKPREVLLEE+GKDWRKID +LEHR VDRPETE E+M
Sbjct: 361 ESSALNNVENVVKPRPKVNPFGDAKPREVLLEEQGKDWRKIDSELEHRRVDRPETEGERM 420

Query: 421 LKEEIDVLRKELE-EGLSLNTNKESVQESAGEMQDLRDRISKKERELETLVLDLDDKIRF 480
           LKEEI+ LRK+LE E      +KES QES    Q+L D I +KE+ L+ L+ +LDDK+RF
Sbjct: 421 LKEEIEELRKKLEKEAAIAPESKESQQESDSNHQNLPDLIREKEKNLDLLIRELDDKVRF 480

Query: 481 GKKATERPGSGAGRAAVFPERPPSQ-GSFDESRSFESIDRPRSRGTGTADVWSRSADDRK 527
             +A ERPGS A R   + ERP S+ GS DESRS ES++RPRS GTG  D W R  DDR+
Sbjct: 481 RPRAVERPGSSASRGGSYSERPHSRAGSIDESRSVESMERPRSHGTG--DNWPRPVDDRR 540

BLAST of Lag0004659 vs. TAIR 10
Match: AT3G26400.1 (eukaryotic translation initiation factor 4B1 )

HSP 1 Score: 489.6 bits (1259), Expect = 3.2e-138
Identity = 320/563 (56.84%), Postives = 377/563 (66.96%), Query Frame = 0

Query: 1   MSKPWG--GIGAWAADAERAEAEEREAQAAAAASQAESKNFPSLREAV------NTKPKK 60
           MSK WG  GIGAWA +AERA+ EE+ A+A AA   A++++FPSLREA           K 
Sbjct: 1   MSKAWGGIGIGAWADEAERAD-EEQAAEATAAT--ADTQSFPSLREAAAATATSGKSRKM 60

Query: 61  KKMTLSEFTMGTLTGPGAGSRQTESQGLTPEEMLRLPTGPKDRSGEEMRSGRLGGGFPSY 120
           KKM+LSEFT G  T PG       S GLT +E+L+LPTGP+ RS EEM+ GRLGGGF SY
Sbjct: 61  KKMSLSEFTTGAYTAPGG----RNSVGLTQQEILQLPTGPRQRSEEEMQPGRLGGGFSSY 120

Query: 121 DRQSMPAGRF-RDRDEGDGSW-----GGGRRSY-GGFDEERRGPPPRASNFDQPSRADEV 180
             +S   GR  RDRD+ DGSW     GGGRR Y GGFD++RRG   R S+F QPSRADEV
Sbjct: 121 GGRS--GGRIGRDRDDSDGSWSGGGGGGGRRPYGGGFDDDRRGNQSRVSDFPQPSRADEV 180

Query: 181 DNWAMTKKS-PTLDSGRQNRYGSLGGGM---------GGIGGGGIASRADEVDNWASSKK 240
           D+W   KK  P+ D GRQ RY   GGG          GG GGGG  SRAD+VDNW + K+
Sbjct: 181 DDWGKEKKPLPSFDQGRQGRYSGDGGGFGGGGSGFGGGGGGGGGGLSRADDVDNWGAGKR 240

Query: 241 AIPSRSSTFGSGFRDREANEPDRWTRGRSRDSDTERPRLVLDPPK----GNVVTNEATRT 300
             P RSSTFGS F D                   ER RLVL+P K    G+       +T
Sbjct: 241 QAPVRSSTFGSSFGD---------------SGQEERRRLVLEPRKVESGGSETPPVVEKT 300

Query: 301 NKPSPFGAARPREEVLAEKGLDWKKLDTEIEAKR----TSRPTSAHSSRPSSAQSSRSEG 360
           +KP+PFGAARPRE+VLAEKGLDWKK+D+EIEAK+    TSRPTSAHSSRPSSAQS+RSE 
Sbjct: 301 SKPNPFGAARPREDVLAEKGLDWKKIDSEIEAKKGSSQTSRPTSAHSSRPSSAQSNRSES 360

Query: 361 PGIQGVENVVKPRPKVNPFGDAKPREVLLEERGKDWRKIDLQLEHRAVDRPETEEEKMLK 420
               G+ NVVKPRPKVNPFGDAKPREVLLEE+GKDWRK+DL+LEHR VDRPETEEEKMLK
Sbjct: 361 ---SGLNNVVKPRPKVNPFGDAKPREVLLEEQGKDWRKMDLELEHRRVDRPETEEEKMLK 420

Query: 421 EEIDVLRKELEEGLSLNTNKESVQE--SAGEMQDLRDRISKKERELETLVLDLDDKIRFG 480
           EEI+ LRK+LE+       KES QE  S     DL + I  KE++LE L  +LDDK+RF 
Sbjct: 421 EEIEELRKKLEKESVAPEIKESDQEPGSNNNHNDLPEIIRGKEKDLEILTRELDDKVRFR 480

Query: 481 KKATERPGSGAGRAAVFPERPPSQ-GSFDESRSFESIDRPRSRGTGTADVWSRSADD-RK 527
           +K  ERPGSGAGR   + ER  S+ GS DE+RSFES +RPRSR  G  D W R A++ R+
Sbjct: 481 QKPVERPGSGAGRTGTYSERTHSRAGSIDETRSFESTERPRSR--GAVDAWVRPANEQRR 532

BLAST of Lag0004659 vs. TAIR 10
Match: AT4G38710.1 (glycine-rich protein )

HSP 1 Score: 139.0 bits (349), Expect = 1.1e-32
Identity = 162/477 (33.96%), Postives = 226/477 (47.38%), Query Frame = 0

Query: 1   MSKPWGGIGAWAADAERAEAE--EREAQAAAAASQAESKNFPSLREAVNTKPKKKK---M 60
           +S  W   GAWA +AE  EAE  ++ +     +S  +S +FPSL  A  TK KKKK   +
Sbjct: 5   VSSVWAKPGAWALEAEEHEAELKQQPSPTNQKSSAEDSSDFPSLAAAATTKTKKKKGQTI 64

Query: 61  TLSEF-TMGTLTGPGAGSRQTESQGLTPEEMLRLPTGPKDRSGEEMRSGRLGGGFPSYDR 120
           +L+EF T GT     A     +++ LT  E++ LPTGP++RS EE+   +LGGGF SY  
Sbjct: 65  SLAEFATYGTAKAKPA----PQTERLTQAELVALPTGPRERSAEELDRSKLGGGFRSYG- 124

Query: 121 QSMPAGRFRDRDEGDGSWGG------GRRSYGGFDEERRGPPPRASNFDQPSRADEVDNW 180
                GR+ D +     WG       G R  GGF+ +R   P R S    PSRADE DNW
Sbjct: 125 ----GGRYGD-ESSSSRWGSSRVSEDGERRGGGFNRDRE--PSRDSG---PSRADEDDNW 184

Query: 181 AMTKKSPTLDSGRQNRYGSLGGGMGGIGGGGIASRADEVDNWASSKKAIPSR--SSTFGS 240
           A  KK P   +G + R    G   GG       S+ADEVD+W S+K + P R  SS  G 
Sbjct: 185 AAAKK-PISGNGFERRERGSG---GGFFESQSQSKADEVDSWVSTKPSEPRRFVSSNGGG 244

Query: 241 G------------FRDREA-------NEPDRWTRGRSRD---------SDTERPRLVLDP 300
           G             R+R++       +E D W R R            S   RPRLVL P
Sbjct: 245 GDRFEKRGSFESLSRNRDSQYGGGGGSESDTWGRRREESGAANGSPPPSGGSRPRLVLQP 304

Query: 301 PKGNVVTNEATRTNKP-----------SPFGAARPREEVLAEKGLDWKKLDTEIEAKRTS 360
               V   E  +   P           +PFG ARPREEVLAEKG DWK++D ++EA++  
Sbjct: 305 RTLPVAVVEVVKPESPVLVIVEKPKGANPFGNARPREEVLAEKGQDWKEIDEKLEAEKL- 364

Query: 361 RPTSAHSSRPSSAQSSRSEGPGIQGVENVVKPRPKVNPFGDAKPREVLLEERGKDWRKID 420
           +  +A   +P    + +S G    G+ N  K   ++      K  E   E+  ++   ++
Sbjct: 365 KDIAAAMEKP----NEKSTGKMGFGLGNGRKDEERIER-SWRKSTEHSEEDAQEEEPAVE 424

Query: 421 LQLEHRAVDRPETEEEKMLKEEIDVLRKELEEGLSLNTNKESVQESAGEMQDLRDRI 425
              +    D+P  EE K  KEE +   + +EE     T  E   E A + ++  D+I
Sbjct: 425 GAKKEETEDKPAVEEAK--KEETEG-EQAVEEAKKEETGGEPAVEEA-KKEETEDKI 452

BLAST of Lag0004659 vs. TAIR 10
Match: AT4G38710.2 (glycine-rich protein )

HSP 1 Score: 138.7 bits (348), Expect = 1.4e-32
Identity = 164/498 (32.93%), Postives = 235/498 (47.19%), Query Frame = 0

Query: 1   MSKPWGGIGAWAADAERAEAE--EREAQAAAAASQAESKNFPSLREAVNTKPKKKK---M 60
           +S  W   GAWA +AE  EAE  ++ +     +S  +S +FPSL  A  TK KKKK   +
Sbjct: 5   VSSVWAKPGAWALEAEEHEAELKQQPSPTNQKSSAEDSSDFPSLAAAATTKTKKKKGQTI 64

Query: 61  TLSEF-TMGTLTGPGAGSRQTESQGLTPEEMLRLPTGPKDRSGEEMRSGRLGGGFPSYDR 120
           +L+EF T GT     A     +++ LT  E++ LPTGP++RS EE+   +LGGGF SY  
Sbjct: 65  SLAEFATYGTAKAKPA----PQTERLTQAELVALPTGPRERSAEELDRSKLGGGFRSYG- 124

Query: 121 QSMPAGRFRDRDEGDGSWGG------GRRSYGGFDEERRGPPPRASNFDQPSRADEVDNW 180
                GR+ D +     WG       G R  GGF+ +R   P R S    PSRADE DNW
Sbjct: 125 ----GGRYGD-ESSSSRWGSSRVSEDGERRGGGFNRDRE--PSRDSG---PSRADEDDNW 184

Query: 181 AMTKKSPTLDSGRQNRYGSLGGGMGGIGGGGIASRADEVDNWASSKKAIPSR--SSTFGS 240
           A  KK P   +G + R    G   GG       S+ADEVD+W S+K + P R  SS  G 
Sbjct: 185 AAAKK-PISGNGFERRERGSG---GGFFESQSQSKADEVDSWVSTKPSEPRRFVSSNGGG 244

Query: 241 G------------FRDREA-------NEPDRWTRGRSRD---------SDTERPRLVLDP 300
           G             R+R++       +E D W R R            S   RPRLVL P
Sbjct: 245 GDRFEKRGSFESLSRNRDSQYGGGGGSESDTWGRRREESGAANGSPPPSGGSRPRLVLQP 304

Query: 301 PKGNVVTNEATRTNKP-----------SPFGAARPREEVLAEKGLDWKKLDTEIEAKRTS 360
               V   E  +   P           +PFG ARPREEVLAEKG DWK++D ++EA++  
Sbjct: 305 RTLPVAVVEVVKPESPVLVIVEKPKGANPFGNARPREEVLAEKGQDWKEIDEKLEAEKL- 364

Query: 361 RPTSAHSSRPSSAQSSR---SEGPGIQGVENVVKPRPKVNPFGDAKPREVLLEERGKDWR 420
           +  +A   +P+   + +     G G +  E + +   K          +VL  E  ++  
Sbjct: 365 KDIAAAMEKPNEKSTGKMGFGLGNGRKDEERIERSWRKSFSLHSYMEVDVLNTEHSEE-- 424

Query: 421 KIDLQLEHRAVDRPETEEEKMLKEEIDVLRKELEEGLSLNTNKESVQESAGEMQDLRDRI 443
             D Q E  AV+  + EE +  K  ++  +KE  EG      +++V+E+  E       +
Sbjct: 425 --DAQEEEPAVEGAKKEETED-KPAVEEAKKEETEG------EQAVEEAKKEETGGEPAV 465

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
XP_011649497.18.7e-26390.00eukaryotic translation initiation factor 4B2 [Cucumis sativus] >KGN62328.1 hypot... [more]
XP_038884361.12.1e-26189.81eukaryotic translation initiation factor 4B2-like [Benincasa hispida][more]
XP_023545450.16.9e-26090.87eukaryotic translation initiation factor 4B2-like [Cucurbita pepo subsp. pepo][more]
KAG6598244.19.0e-26090.68Eukaryotic translation initiation factor 4B1, partial [Cucurbita argyrosperma su... [more]
XP_022996764.14.5e-25990.49eukaryotic translation initiation factor 4B2-like [Cucurbita maxima][more]
Match NameE-valueIdentityDescription
Q9SAD74.3e-14858.48Eukaryotic translation initiation factor 4B2 OS=Arabidopsis thaliana OX=3702 GN=... [more]
Q9LIN54.5e-13756.84Eukaryotic translation initiation factor 4B1 OS=Arabidopsis thaliana OX=3702 GN=... [more]
Q9AUJ71.6e-9444.27Eukaryotic translation initiation factor 4B1 OS=Triticum aestivum OX=4565 GN=EIF... [more]
Q9SZP81.5e-3133.96Eukaryotic translation initiation factor 4B3 OS=Arabidopsis thaliana OX=3702 GN=... [more]
Match NameE-valueIdentityDescription
A0A0A0LKJ94.2e-26390.00Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_2G349620 PE=4 SV=1[more]
A0A6J1K9L62.2e-25990.49eukaryotic translation initiation factor 4B2-like OS=Cucurbita maxima OX=3661 GN... [more]
A0A1S3BA921.1e-25884.97eukaryotic translation initiation factor 4B2 OS=Cucumis melo OX=3656 GN=LOC10348... [more]
A0A6J1HBJ61.8e-25890.11eukaryotic translation initiation factor 4B2-like OS=Cucurbita moschata OX=3662 ... [more]
A0A6J1KMX73.8e-25689.35eukaryotic translation initiation factor 4B2-like OS=Cucurbita maxima OX=3661 GN... [more]
Match NameE-valueIdentityDescription
AT1G13020.13.1e-14958.48eukaryotic initiation factor 4B2 [more]
AT3G26400.13.2e-13856.84eukaryotic translation initiation factor 4B1 [more]
AT4G38710.11.1e-3233.96glycine-rich protein [more]
AT4G38710.21.4e-3232.93glycine-rich protein [more]
InterPro
Analysis Name: InterPro Annotations of Sponge gourd (AG-4) v1
Date Performed: 2022-08-01
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
NoneNo IPR availableCOILSCoilCoilcoord: 15..37
NoneNo IPR availableCOILSCoilCoilcoord: 410..437
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 59..81
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 118..132
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 447..526
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 214..228
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 1..351
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 310..329
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 496..526
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 230..255
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 283..309
NoneNo IPR availablePANTHERPTHR32091:SF20EUKARYOTIC TRANSLATION INITIATION FACTOR 4B1coord: 1..526
IPR010433Plant specific eukaryotic initiation factor 4BPFAMPF06273eIF-4Bcoord: 1..512
e-value: 3.3E-231
score: 768.7
IPR010433Plant specific eukaryotic initiation factor 4BPANTHERPTHR32091EUKARYOTIC TRANSLATION INITIATION FACTOR 4Bcoord: 1..526

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Lag0004659.1Lag0004659.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0006413 translational initiation
molecular_function GO:0003729 mRNA binding
molecular_function GO:0003743 translation initiation factor activity