Lag0004619 (gene) Sponge gourd (AG‐4) v1

Overview
NameLag0004619
Typegene
OrganismLuffa acutangula (Sponge gourd (AG‐4) v1)
DescriptionUPF0481 protein At3g47200-like
Locationchr6: 5504936 .. 5505921 (+)
RNA-Seq ExpressionLag0004619
SyntenyLag0004619
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonCDSpolypeptide
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGGCTTTGCGCAGCGTGAACCCAAAAGCTTATACTCCTCAAGTCATTTCCATCGGCCCTTTTCATTATGAAAAGAACAATAACCCGATGGTTATGGAGAAAAAAGGCGGCTACGTTCTAATCTTTCTTGCACTTGCAAAACTAAGCTGGGATCAGATTATAGAAAAAGTCATGAGTTGGGAAGAAAGAGCTCGCAATTCTTACGACGAACCAATCAAGATGGACAGAGGTGAGTTGGTGCAACTTCTGATCTTGGATGGTTGTTTCGTGGTTATGTACATCATTGGCTCTCTATTCCCGGAGTTTCGAGACATCGACATGGGGTTTTTATGGAGATTCAACAATGGAATATTCAAAGATTTGTTATTGCTTGAAAACCAGCTTCCTTTCTTCCTTCTACACGCTCTTTACAACTTGTGCGCCTCCTCTCAACCTTCACTCAGACATGTCTCTTTCATCGAACTCGTCCGTTCATATTTTCGCAGGGCTCGTGAAGGATTGAGTTATTTTGAAAGAGACTTTGAAATAGACGCCAGTGAAGTGAATCATCTCAGAAATCATAATAGACAGACAGTTTCGAAATCAGTCTAAGAAACGTCATAGCTTATGAACACTGCCACCGTAAGAGTGGGGATCACGTGAGCAACTTCGCCATGTTCATGCATTTCTTGATAAACACAAACAAAGACGTGAGTTTGCTCATTCACGACGGGATTATACACAACCATTCGGGAAGCAGCACTAACGAAATTAGTAGATTGTTCAACGACCTTTGTAAGCACACAATGGTTGAAAGAAATCTGTACAACAAAGAATGCTTGAAAATGGAAGAATACTGCAAGCGCCGTCGGTATCAGTGGATGACTTCGTTGAAACGTGACTATTTCAGTACCCCGTGGGCCTTTATCTCCTTCCTCGCTGCTGTCTTCCTCCTTTCGCTCACTCTCCTGCAAGCTGTGGTAGATCTCATCGATTTAAAAAAGTGA

mRNA sequence

ATGGCTTTGCGCAGCGTGAACCCAAAAGCTTATACTCCTCAAGTCATTTCCATCGGCCCTTTTCATTATGAAAAGAACAATAACCCGATGGTTATGGAGAAAAAAGGCGGCTACGTTCTAATCTTTCTTGCACTTGCAAAACTAAGCTGGGATCAGATTATAGAAAAAGTCATGAGTTGGGAAGAAAGAGCTCGCAATTCTTACGACGAACCAATCAAGATGGACAGAGGTGAGTTGGTGCAACTTCTGATCTTGGATGGTTGTTTCGTGGTTATGTACATCATTGGCTCTCTATTCCCGGAGTTTCGAGACATCGACATGGGGTTTTTATGGAGATTCAACAATGGAATATTCAAAGATTTGTTATTGCTTGAAAACCAGCTTCCTTTCTTCCTTCTACACGCTCTTTACAACTTGTGCGCCTCCTCTCAACCTTCACTCAGACATGTCTCTTTCATCGAACTCGTCCACAGTTTCGAAATCAGTCTAAGAAACGTCATAGCTTATGAACACTGCCACCGTAAGAGTGGGGATCACGTGAGCAACTTCGCCATGTTCATGCATTTCTTGATAAACACAAACAAAGACGTGAGTTTGCTCATTCACGACGGGATTATACACAACCATTCGGGAAGCAGCACTAACGAAATTAGTAGATTGTTCAACGACCTTTGTAAGCACACAATGGTTGAAAGAAATCTGTACAACAAAGAATGCTTGAAAATGGAAGAATACTGCAAGCGCCGTCGGTATCAGTGGATGACTTCGTTGAAACGTGACTATTTCAGTACCCCGTGGGCCTTTATCTCCTTCCTCGCTGCTGTCTTCCTCCTTTCGCTCACTCTCCTGCAAGCTGTGGTAGATCTCATCGATTTAAAAAAGTGA

Coding sequence (CDS)

ATGGCTTTGCGCAGCGTGAACCCAAAAGCTTATACTCCTCAAGTCATTTCCATCGGCCCTTTTCATTATGAAAAGAACAATAACCCGATGGTTATGGAGAAAAAAGGCGGCTACGTTCTAATCTTTCTTGCACTTGCAAAACTAAGCTGGGATCAGATTATAGAAAAAGTCATGAGTTGGGAAGAAAGAGCTCGCAATTCTTACGACGAACCAATCAAGATGGACAGAGGTGAGTTGGTGCAACTTCTGATCTTGGATGGTTGTTTCGTGGTTATGTACATCATTGGCTCTCTATTCCCGGAGTTTCGAGACATCGACATGGGGTTTTTATGGAGATTCAACAATGGAATATTCAAAGATTTGTTATTGCTTGAAAACCAGCTTCCTTTCTTCCTTCTACACGCTCTTTACAACTTGTGCGCCTCCTCTCAACCTTCACTCAGACATGTCTCTTTCATCGAACTCGTCCACAGTTTCGAAATCAGTCTAAGAAACGTCATAGCTTATGAACACTGCCACCGTAAGAGTGGGGATCACGTGAGCAACTTCGCCATGTTCATGCATTTCTTGATAAACACAAACAAAGACGTGAGTTTGCTCATTCACGACGGGATTATACACAACCATTCGGGAAGCAGCACTAACGAAATTAGTAGATTGTTCAACGACCTTTGTAAGCACACAATGGTTGAAAGAAATCTGTACAACAAAGAATGCTTGAAAATGGAAGAATACTGCAAGCGCCGTCGGTATCAGTGGATGACTTCGTTGAAACGTGACTATTTCAGTACCCCGTGGGCCTTTATCTCCTTCCTCGCTGCTGTCTTCCTCCTTTCGCTCACTCTCCTGCAAGCTGTGGTAGATCTCATCGATTTAAAAAAGTGA

Protein sequence

MALRSVNPKAYTPQVISIGPFHYEKNNNPMVMEKKGGYVLIFLALAKLSWDQIIEKVMSWEERARNSYDEPIKMDRGELVQLLILDGCFVVMYIIGSLFPEFRDIDMGFLWRFNNGIFKDLLLLENQLPFFLLHALYNLCASSQPSLRHVSFIELVHSFEISLRNVIAYEHCHRKSGDHVSNFAMFMHFLINTNKDVSLLIHDGIIHNHSGSSTNEISRLFNDLCKHTMVERNLYNKECLKMEEYCKRRRYQWMTSLKRDYFSTPWAFISFLAAVFLLSLTLLQAVVDLIDLKK
Homology
BLAST of Lag0004619 vs. NCBI nr
Match: XP_008445209.1 (PREDICTED: UPF0481 protein At3g47200-like [Cucumis melo])

HSP 1 Score: 366.7 bits (940), Expect = 1.9e-97
Identity = 209/392 (53.32%), Postives = 238/392 (60.71%), Query Frame = 0

Query: 3   LRSVNPKAYTPQVISIGPFHYEKNNNPM-VMEKKGGYVLIFLALAKLSWDQIIEKVMSWE 62
           LR+ NPKAY+PQVISIGP HY +  N + + EKKG YVL FL +AKL W+++I K + WE
Sbjct: 59  LRNGNPKAYSPQVISIGPLHYYRTQNDLTIKEKKGSYVLNFLTVAKLGWNEMINKFLCWE 118

Query: 63  ERARNSYDEPIKMDRGELVQLLILDGCFVVMYIIGSLFPEFRDIDMGFLWRFNNGIFKDL 122
           ERARN Y E IKM+R E +QLLI D CFVVMYIIGS+  EFRD+D  FLWRF+NGIFKDL
Sbjct: 119 ERARNYYVETIKMERDEFIQLLIYDSCFVVMYIIGSMVAEFRDLDTSFLWRFSNGIFKDL 178

Query: 123 LLLENQLPFFLLHALYNLCASSQPSLRHVSFIELVH------------------------ 182
           LLLENQLPFFLLH LYNLCA +QPSL+ +SFIEL+                         
Sbjct: 179 LLLENQLPFFLLHHLYNLCAFAQPSLKDISFIELLRGYFTEVREGMSYVNEGYLDIDANE 238

Query: 183 ------------------------------------------------------------ 242
                                                                       
Sbjct: 239 VNHLVDFLRIHLTQPRHIPHFLDFSVDDFLSSWPLTATELHDCGISFHGQKRCMMNVNFR 298

Query: 243 ---------------SFEISLRNVIAYEHCHRKSGDHVSNFAMFMHFLINTNKDVSLLIH 295
                          SFEI  RN+IAYE+CH KS D VSNF MFMHFLINTNKDVSLL+ 
Sbjct: 299 ERNGVLKMPKIIIDDSFEILFRNMIAYEYCHLKSKD-VSNFGMFMHFLINTNKDVSLLVD 358

BLAST of Lag0004619 vs. NCBI nr
Match: KAA0064930.1 (UPF0481 protein [Cucumis melo var. makuwa])

HSP 1 Score: 365.2 bits (936), Expect = 5.5e-97
Identity = 208/392 (53.06%), Postives = 238/392 (60.71%), Query Frame = 0

Query: 3   LRSVNPKAYTPQVISIGPFHYEKNNNPM-VMEKKGGYVLIFLALAKLSWDQIIEKVMSWE 62
           LR+ NPKAY+PQVISIGP HY +  N + + EKKG YVL FL +AKL W+++I K + WE
Sbjct: 59  LRNGNPKAYSPQVISIGPLHYYRTQNDLTIKEKKGSYVLNFLTVAKLGWNEMINKFLCWE 118

Query: 63  ERARNSYDEPIKMDRGELVQLLILDGCFVVMYIIGSLFPEFRDIDMGFLWRFNNGIFKDL 122
           ERARN Y E IKM+R E +QLLI D CFVVMYIIGS+  EFRD+D  FLWRF+NGIFKDL
Sbjct: 119 ERARNYYVETIKMERDEFIQLLIYDSCFVVMYIIGSMVAEFRDLDTSFLWRFSNGIFKDL 178

Query: 123 LLLENQLPFFLLHALYNLCASSQPSLRHVSFIELVH------------------------ 182
           LLLENQLPFFLLH LYNLCA +QPSL+ +SFIEL+                         
Sbjct: 179 LLLENQLPFFLLHHLYNLCAFAQPSLKDISFIELLRGYFTEVREGMSYVNEGYLDIDANE 238

Query: 183 ------------------------------------------------------------ 242
                                                                       
Sbjct: 239 VNHLVDFLRIHLTQPRHIPHFLDFSVDDFLSSWPLTATELHDCGISFHGQKRCMMNVNFR 298

Query: 243 ---------------SFEISLRNVIAYEHCHRKSGDHVSNFAMFMHFLINTNKDVSLLIH 295
                          SFEI  RN+IAYE+CH KS D VSNF MFMHFLINTNKDVSLL+ 
Sbjct: 299 ERNGVLKMPKIIIDDSFEILFRNMIAYEYCHLKSKD-VSNFGMFMHFLINTNKDVSLLVD 358

BLAST of Lag0004619 vs. NCBI nr
Match: XP_004138858.1 (UPF0481 protein At3g47200 [Cucumis sativus] >KGN62944.1 hypothetical protein Csa_022605 [Cucumis sativus])

HSP 1 Score: 356.7 bits (914), Expect = 1.9e-94
Identity = 201/391 (51.41%), Postives = 235/391 (60.10%), Query Frame = 0

Query: 3   LRSVNPKAYTPQVISIGPFHYEKNNNPMVMEKKGGYVLIFLALAKLSWDQIIEKVMSWEE 62
           LR  NPKAY+PQVISIGP HY +  N ++ EKKG YVL FL +AKL W+++I+K +SWEE
Sbjct: 60  LRKGNPKAYSPQVISIGPLHYYRTQNDLIKEKKGSYVLNFLTVAKLDWNEMIKKFLSWEE 119

Query: 63  RARNSYDEPIKMDRGELVQLLILDGCFVVMYIIGSLFPEFRDIDMGFLWRFNNGIFKDLL 122
           RARN Y E I+M R E +QLLI D CFVVMY+IGS+  EFRD+D  FLWRF+NGIFKDLL
Sbjct: 120 RARNYYVETIEMKRDEFIQLLIYDSCFVVMYVIGSMVAEFRDLDTSFLWRFSNGIFKDLL 179

Query: 123 LLENQLPFFLLHALYNLCASSQPSLRHVSFIELVH------------------------- 182
           LLENQLPFFLL+ LYNLCAS+QPSL+ +SFIEL+                          
Sbjct: 180 LLENQLPFFLLNHLYNLCASAQPSLKDISFIELLRGYFSKVREGMSYVKEGYFDIDASAV 239

Query: 183 ------------------------------------------------------------ 242
                                                                       
Sbjct: 240 NHLVDFLRIHLTQPRHIPHFFGLSVDDFLSSWPLTATELHECGISFHGQKKCMMNVSFKE 299

Query: 243 --------------SFEISLRNVIAYEHCHRKSGDHVSNFAMFMHFLINTNKDVSLLIHD 295
                         SFEI  RN+IAYE+CH KS D  SNF MFMHFLINTN+DVSLL+ D
Sbjct: 300 RRGVLKMPKIIIDDSFEILFRNMIAYEYCHLKSKD-ASNFGMFMHFLINTNEDVSLLVDD 359

BLAST of Lag0004619 vs. NCBI nr
Match: XP_022961896.1 (UPF0481 protein At3g47200-like isoform X2 [Cucurbita moschata])

HSP 1 Score: 256.5 bits (654), Expect = 2.7e-64
Identity = 154/351 (43.87%), Postives = 206/351 (58.69%), Query Frame = 0

Query: 3   LRSVNPKAYTPQVISIGPFHYEKNNNPMVMEKKGGYVLIFLALAKLSWDQIIEKVMSWEE 62
           LRS+ P+AYTP VISIGP H     +      K  Y+  FL L KL  + I+E V +WE+
Sbjct: 182 LRSIKPEAYTPTVISIGPLH-SGRKDLTANSLKPMYLQNFLNLTKLPTNTIVETVKTWEK 241

Query: 63  RARNSYDEPIKMDRGELVQLLILDGCFVVMYIIGSLFPEFRDIDMGFLWRFNNGIFKDLL 122
           RAR  Y E I+M+R E V+LL+ DGCFVVM++IG  F E R  DM  LW+F   +F DL+
Sbjct: 242 RARYCYAESIEMNRDEFVELLVFDGCFVVMHLIGYSFFELRASDMSNLWKFWYELFCDLI 301

Query: 123 LLENQLPFFLLHALYNLCASSQPSLRHVS---FIELVH---------------------- 182
           LLENQLPFFLL +LY+LCASSQP L+ V    F++L+                       
Sbjct: 302 LLENQLPFFLLQSLYDLCASSQPLLKGVQVNHFVDLLRLHFTHTRSDETSFQHTFWPPNA 361

Query: 183 ----------------------------------SFEISLRNVIAYEHCHRKS--GDHVS 242
                                              FE  +RN+IAYE CH  S   + VS
Sbjct: 362 TKLHECGVIFKMGKGIAFKDQGGCLQLPQINIYDDFEKRVRNLIAYEQCHIGSELRNEVS 421

Query: 243 NFAMFMHFLINTNKDVSLLIHDGIIHNHSGSSTNEISRLFNDLCKHTMVERNLYNKECLK 293
           NFA+FM  L+ T++DV LLI  GIIHN+ G S NE+++LFN+L KH     N YN +C +
Sbjct: 422 NFAVFMQCLVQTDQDVKLLIEGGIIHNNFG-SINEVTQLFNNLGKHICPGINSYNFDCKR 481

BLAST of Lag0004619 vs. NCBI nr
Match: XP_022961897.1 (UPF0481 protein At3g47200-like isoform X3 [Cucurbita moschata] >XP_022961898.1 UPF0481 protein At3g47200-like isoform X3 [Cucurbita moschata] >XP_022961899.1 UPF0481 protein At3g47200-like isoform X3 [Cucurbita moschata])

HSP 1 Score: 253.8 bits (647), Expect = 1.8e-63
Identity = 158/383 (41.25%), Postives = 207/383 (54.05%), Query Frame = 0

Query: 3   LRSVNPKAYTPQVISIGPFHYEKNNNPMVMEKKGGYVLIFLALAKLSWDQIIEKVMSWEE 62
           LRS+ P+AYTP VISIGP H     +      K  Y+  FL L KL  + I+E V +WE+
Sbjct: 83  LRSIKPEAYTPTVISIGPLH-SGRKDLTANSLKPMYLQNFLNLTKLPTNTIVETVKTWEK 142

Query: 63  RARNSYDEPIKMDRGELVQLLILDGCFVVMYIIGSLFPEFRDIDMGFLWRFNNGIFKDLL 122
           RAR  Y E I+M+R E V+LL+ DGCFVVM++IG  F E R  DM  LW+F   +F DL+
Sbjct: 143 RARYCYAESIEMNRDEFVELLVFDGCFVVMHLIGYSFFELRASDMSNLWKFWYELFCDLI 202

Query: 123 LLENQLPFFLLHALYNLCASSQPSLRHVSFIELVH------------------------- 182
           LLENQLPFFLL +LY+LCASSQP L+ V FIELVH                         
Sbjct: 203 LLENQLPFFLLQSLYDLCASSQPLLKGVHFIELVHQYFIESHKGGLFSLKEHVLLAGIGV 262

Query: 183 ------------------------------------------------------------ 242
                                                                       
Sbjct: 263 QVNHFVDLLRLHFTHTRSDETSFQHTFWPPNATKLHECGVIFKMGKGIAFKDQGGCLQLP 322

Query: 243 ------SFEISLRNVIAYEHCHRKS--GDHVSNFAMFMHFLINTNKDVSLLIHDGIIHNH 293
                  FE  +RN+IAYE CH  S   + VSNFA+FM  L+ T++DV LLI  GIIHN+
Sbjct: 323 QINIYDDFEKRVRNLIAYEQCHIGSELRNEVSNFAVFMQCLVQTDQDVKLLIEGGIIHNN 382

BLAST of Lag0004619 vs. ExPASy Swiss-Prot
Match: Q9SD53 (UPF0481 protein At3g47200 OS=Arabidopsis thaliana OX=3702 GN=At3g47200 PE=2 SV=1)

HSP 1 Score: 105.1 bits (261), Expect = 1.3e-21
Identity = 101/402 (25.12%), Postives = 169/402 (42.04%), Query Frame = 0

Query: 5   SVNPKAYTPQVISIGPFHYEKNNNPMVMEKKGGYVLIFLALAK---LSWDQIIEKVMSWE 64
           ++NPKAY P+V+SIGP+HY + +  M+ + K   + +FL  AK   +  + +++ V+  E
Sbjct: 57  ALNPKAYKPKVVSIGPYHYGEKHLQMIQQHKPRLLQLFLDEAKKKDVEENVLVKAVVDLE 116

Query: 65  ERARNSYDEPIKMDRGELVQLLILDGCFVVM-YIIGSLFPEFRDIDMGFLWRFNNGIFKD 124
           ++ R SY E +K    +L+ +++LDGCF++M ++I S   E  +  +  +    + I  D
Sbjct: 117 DKIRKSYSEELKTGH-DLMFMMVLDGCFILMVFLIMSGNIELSEDPIFSIPWLLSSIQSD 176

Query: 125 LLLLENQLPFFLLHALY------------------------------------------- 184
           LLLLENQ+PFF+L  LY                                           
Sbjct: 177 LLLLENQVPFFVLQTLYVGSKIGVSSDLNRIAFHFFKNPIDKEGSYWEKHRNYKAKHLLD 236

Query: 185 --------NLCASSQPSLRHVSF---------------------------------IELV 244
                   N   S + S  HV                                     L 
Sbjct: 237 LIRETFLPNTSESDKASSPHVQVQLHEGKSGNVPSVDSKAVPLILSAKRLRLQGIKFRLR 296

Query: 245 HSFEISLRNV-------------------------IAYEHCHRKSGDHVSNFAMFMHFLI 291
            S E S+ NV                         +A+E  +  S + ++ + +FM  L+
Sbjct: 297 RSKEDSILNVRLKKNKLQIPQLRFDGFISSFFLNCVAFEQFYTDSSNEITTYIVFMGCLL 356

BLAST of Lag0004619 vs. ExPASy TrEMBL
Match: A0A1S3BD00 (UPF0481 protein At3g47200-like OS=Cucumis melo OX=3656 GN=LOC103488308 PE=4 SV=1)

HSP 1 Score: 366.7 bits (940), Expect = 9.1e-98
Identity = 209/392 (53.32%), Postives = 238/392 (60.71%), Query Frame = 0

Query: 3   LRSVNPKAYTPQVISIGPFHYEKNNNPM-VMEKKGGYVLIFLALAKLSWDQIIEKVMSWE 62
           LR+ NPKAY+PQVISIGP HY +  N + + EKKG YVL FL +AKL W+++I K + WE
Sbjct: 59  LRNGNPKAYSPQVISIGPLHYYRTQNDLTIKEKKGSYVLNFLTVAKLGWNEMINKFLCWE 118

Query: 63  ERARNSYDEPIKMDRGELVQLLILDGCFVVMYIIGSLFPEFRDIDMGFLWRFNNGIFKDL 122
           ERARN Y E IKM+R E +QLLI D CFVVMYIIGS+  EFRD+D  FLWRF+NGIFKDL
Sbjct: 119 ERARNYYVETIKMERDEFIQLLIYDSCFVVMYIIGSMVAEFRDLDTSFLWRFSNGIFKDL 178

Query: 123 LLLENQLPFFLLHALYNLCASSQPSLRHVSFIELVH------------------------ 182
           LLLENQLPFFLLH LYNLCA +QPSL+ +SFIEL+                         
Sbjct: 179 LLLENQLPFFLLHHLYNLCAFAQPSLKDISFIELLRGYFTEVREGMSYVNEGYLDIDANE 238

Query: 183 ------------------------------------------------------------ 242
                                                                       
Sbjct: 239 VNHLVDFLRIHLTQPRHIPHFLDFSVDDFLSSWPLTATELHDCGISFHGQKRCMMNVNFR 298

Query: 243 ---------------SFEISLRNVIAYEHCHRKSGDHVSNFAMFMHFLINTNKDVSLLIH 295
                          SFEI  RN+IAYE+CH KS D VSNF MFMHFLINTNKDVSLL+ 
Sbjct: 299 ERNGVLKMPKIIIDDSFEILFRNMIAYEYCHLKSKD-VSNFGMFMHFLINTNKDVSLLVD 358

BLAST of Lag0004619 vs. ExPASy TrEMBL
Match: A0A5A7VCL1 (UPF0481 protein OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold82G002480 PE=4 SV=1)

HSP 1 Score: 365.2 bits (936), Expect = 2.6e-97
Identity = 208/392 (53.06%), Postives = 238/392 (60.71%), Query Frame = 0

Query: 3   LRSVNPKAYTPQVISIGPFHYEKNNNPM-VMEKKGGYVLIFLALAKLSWDQIIEKVMSWE 62
           LR+ NPKAY+PQVISIGP HY +  N + + EKKG YVL FL +AKL W+++I K + WE
Sbjct: 59  LRNGNPKAYSPQVISIGPLHYYRTQNDLTIKEKKGSYVLNFLTVAKLGWNEMINKFLCWE 118

Query: 63  ERARNSYDEPIKMDRGELVQLLILDGCFVVMYIIGSLFPEFRDIDMGFLWRFNNGIFKDL 122
           ERARN Y E IKM+R E +QLLI D CFVVMYIIGS+  EFRD+D  FLWRF+NGIFKDL
Sbjct: 119 ERARNYYVETIKMERDEFIQLLIYDSCFVVMYIIGSMVAEFRDLDTSFLWRFSNGIFKDL 178

Query: 123 LLLENQLPFFLLHALYNLCASSQPSLRHVSFIELVH------------------------ 182
           LLLENQLPFFLLH LYNLCA +QPSL+ +SFIEL+                         
Sbjct: 179 LLLENQLPFFLLHHLYNLCAFAQPSLKDISFIELLRGYFTEVREGMSYVNEGYLDIDANE 238

Query: 183 ------------------------------------------------------------ 242
                                                                       
Sbjct: 239 VNHLVDFLRIHLTQPRHIPHFLDFSVDDFLSSWPLTATELHDCGISFHGQKRCMMNVNFR 298

Query: 243 ---------------SFEISLRNVIAYEHCHRKSGDHVSNFAMFMHFLINTNKDVSLLIH 295
                          SFEI  RN+IAYE+CH KS D VSNF MFMHFLINTNKDVSLL+ 
Sbjct: 299 ERNGVLKMPKIIIDDSFEILFRNMIAYEYCHLKSKD-VSNFGMFMHFLINTNKDVSLLVD 358

BLAST of Lag0004619 vs. ExPASy TrEMBL
Match: A0A0A0LPK8 (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_2G381700 PE=4 SV=1)

HSP 1 Score: 356.7 bits (914), Expect = 9.4e-95
Identity = 201/391 (51.41%), Postives = 235/391 (60.10%), Query Frame = 0

Query: 3   LRSVNPKAYTPQVISIGPFHYEKNNNPMVMEKKGGYVLIFLALAKLSWDQIIEKVMSWEE 62
           LR  NPKAY+PQVISIGP HY +  N ++ EKKG YVL FL +AKL W+++I+K +SWEE
Sbjct: 60  LRKGNPKAYSPQVISIGPLHYYRTQNDLIKEKKGSYVLNFLTVAKLDWNEMIKKFLSWEE 119

Query: 63  RARNSYDEPIKMDRGELVQLLILDGCFVVMYIIGSLFPEFRDIDMGFLWRFNNGIFKDLL 122
           RARN Y E I+M R E +QLLI D CFVVMY+IGS+  EFRD+D  FLWRF+NGIFKDLL
Sbjct: 120 RARNYYVETIEMKRDEFIQLLIYDSCFVVMYVIGSMVAEFRDLDTSFLWRFSNGIFKDLL 179

Query: 123 LLENQLPFFLLHALYNLCASSQPSLRHVSFIELVH------------------------- 182
           LLENQLPFFLL+ LYNLCAS+QPSL+ +SFIEL+                          
Sbjct: 180 LLENQLPFFLLNHLYNLCASAQPSLKDISFIELLRGYFSKVREGMSYVKEGYFDIDASAV 239

Query: 183 ------------------------------------------------------------ 242
                                                                       
Sbjct: 240 NHLVDFLRIHLTQPRHIPHFFGLSVDDFLSSWPLTATELHECGISFHGQKKCMMNVSFKE 299

Query: 243 --------------SFEISLRNVIAYEHCHRKSGDHVSNFAMFMHFLINTNKDVSLLIHD 295
                         SFEI  RN+IAYE+CH KS D  SNF MFMHFLINTN+DVSLL+ D
Sbjct: 300 RRGVLKMPKIIIDDSFEILFRNMIAYEYCHLKSKD-ASNFGMFMHFLINTNEDVSLLVDD 359

BLAST of Lag0004619 vs. ExPASy TrEMBL
Match: A0A6J1HBL7 (UPF0481 protein At3g47200-like isoform X2 OS=Cucurbita moschata OX=3662 GN=LOC111462529 PE=4 SV=1)

HSP 1 Score: 256.5 bits (654), Expect = 1.3e-64
Identity = 154/351 (43.87%), Postives = 206/351 (58.69%), Query Frame = 0

Query: 3   LRSVNPKAYTPQVISIGPFHYEKNNNPMVMEKKGGYVLIFLALAKLSWDQIIEKVMSWEE 62
           LRS+ P+AYTP VISIGP H     +      K  Y+  FL L KL  + I+E V +WE+
Sbjct: 182 LRSIKPEAYTPTVISIGPLH-SGRKDLTANSLKPMYLQNFLNLTKLPTNTIVETVKTWEK 241

Query: 63  RARNSYDEPIKMDRGELVQLLILDGCFVVMYIIGSLFPEFRDIDMGFLWRFNNGIFKDLL 122
           RAR  Y E I+M+R E V+LL+ DGCFVVM++IG  F E R  DM  LW+F   +F DL+
Sbjct: 242 RARYCYAESIEMNRDEFVELLVFDGCFVVMHLIGYSFFELRASDMSNLWKFWYELFCDLI 301

Query: 123 LLENQLPFFLLHALYNLCASSQPSLRHVS---FIELVH---------------------- 182
           LLENQLPFFLL +LY+LCASSQP L+ V    F++L+                       
Sbjct: 302 LLENQLPFFLLQSLYDLCASSQPLLKGVQVNHFVDLLRLHFTHTRSDETSFQHTFWPPNA 361

Query: 183 ----------------------------------SFEISLRNVIAYEHCHRKS--GDHVS 242
                                              FE  +RN+IAYE CH  S   + VS
Sbjct: 362 TKLHECGVIFKMGKGIAFKDQGGCLQLPQINIYDDFEKRVRNLIAYEQCHIGSELRNEVS 421

Query: 243 NFAMFMHFLINTNKDVSLLIHDGIIHNHSGSSTNEISRLFNDLCKHTMVERNLYNKECLK 293
           NFA+FM  L+ T++DV LLI  GIIHN+ G S NE+++LFN+L KH     N YN +C +
Sbjct: 422 NFAVFMQCLVQTDQDVKLLIEGGIIHNNFG-SINEVTQLFNNLGKHICPGINSYNFDCKR 481

BLAST of Lag0004619 vs. ExPASy TrEMBL
Match: A0A6J1HBC3 (UPF0481 protein At3g47200-like isoform X3 OS=Cucurbita moschata OX=3662 GN=LOC111462529 PE=4 SV=1)

HSP 1 Score: 253.8 bits (647), Expect = 8.6e-64
Identity = 158/383 (41.25%), Postives = 207/383 (54.05%), Query Frame = 0

Query: 3   LRSVNPKAYTPQVISIGPFHYEKNNNPMVMEKKGGYVLIFLALAKLSWDQIIEKVMSWEE 62
           LRS+ P+AYTP VISIGP H     +      K  Y+  FL L KL  + I+E V +WE+
Sbjct: 83  LRSIKPEAYTPTVISIGPLH-SGRKDLTANSLKPMYLQNFLNLTKLPTNTIVETVKTWEK 142

Query: 63  RARNSYDEPIKMDRGELVQLLILDGCFVVMYIIGSLFPEFRDIDMGFLWRFNNGIFKDLL 122
           RAR  Y E I+M+R E V+LL+ DGCFVVM++IG  F E R  DM  LW+F   +F DL+
Sbjct: 143 RARYCYAESIEMNRDEFVELLVFDGCFVVMHLIGYSFFELRASDMSNLWKFWYELFCDLI 202

Query: 123 LLENQLPFFLLHALYNLCASSQPSLRHVSFIELVH------------------------- 182
           LLENQLPFFLL +LY+LCASSQP L+ V FIELVH                         
Sbjct: 203 LLENQLPFFLLQSLYDLCASSQPLLKGVHFIELVHQYFIESHKGGLFSLKEHVLLAGIGV 262

Query: 183 ------------------------------------------------------------ 242
                                                                       
Sbjct: 263 QVNHFVDLLRLHFTHTRSDETSFQHTFWPPNATKLHECGVIFKMGKGIAFKDQGGCLQLP 322

Query: 243 ------SFEISLRNVIAYEHCHRKS--GDHVSNFAMFMHFLINTNKDVSLLIHDGIIHNH 293
                  FE  +RN+IAYE CH  S   + VSNFA+FM  L+ T++DV LLI  GIIHN+
Sbjct: 323 QINIYDDFEKRVRNLIAYEQCHIGSELRNEVSNFAVFMQCLVQTDQDVKLLIEGGIIHNN 382

BLAST of Lag0004619 vs. TAIR 10
Match: AT4G31980.1 (unknown protein; CONTAINS InterPro DOMAIN/s: Protein of unknown function DUF247, plant (InterPro:IPR004158), Protein of unknown function DUF862, eukaryotic (InterPro:IPR008580); BEST Arabidopsis thaliana protein match is: Plant protein of unknown function (DUF247) (TAIR:AT5G11290.1); Has 1967 Blast hits to 1844 proteins in 183 species: Archae - 0; Bacteria - 6; Metazoa - 223; Fungi - 83; Plants - 1477; Viruses - 0; Other Eukaryotes - 178 (source: NCBI BLink). )

HSP 1 Score: 135.2 bits (339), Expect = 8.6e-32
Identity = 105/382 (27.49%), Postives = 169/382 (44.24%), Query Frame = 0

Query: 3   LRSVNPKAYTPQVISIGPFHYEKNNNPMVMEKKGGYVLIFLALAKLSWDQIIEKVMSWEE 62
           LR +NP AYTP+++S GP H  K     + ++K  Y+L F+     S + ++    +WE+
Sbjct: 302 LRRLNPDAYTPRLVSFGPLHRGKEELQAMEDQKYRYLLSFIPRTNSSLEDLVRLARTWEQ 361

Query: 63  RARNSYDEPIKMDRGELVQLLILDGCFVVMYIIGSLFPEFR---DIDMGFLWRFNNGIFK 122
            AR+ Y E +K+   E V++L++DG F+V  ++ S +P  R   D   G      + + +
Sbjct: 362 NARSCYAEDVKLHSDEFVEMLVVDGSFLVELLLRSHYPRLRGENDRIFGNSMMITD-VCR 421

Query: 123 DLLLLENQLPFFLLHALY----NLCASSQPSL-----RHVS-----------------FI 182
           D++L+ENQLPFF++  ++    N      PS+     RH S                 F+
Sbjct: 422 DMILIENQLPFFVVKEIFLLLLNYYQQGTPSIIQLAQRHFSYFLSRIDDEKFITEPEHFV 481

Query: 183 ELVHS-----FEISL--------------------------------------------- 242
           +L+ S     F I L                                             
Sbjct: 482 DLLRSCYLPQFPIKLEYTTVKVDNAPEATELHTAGVRFKPAETSSCLLDISFADGVLKIP 541

Query: 243 ------------RNVIAYEHCHRKSGDHVSNFAMFMHFLINTNKDVSLLIHDGIIHNHSG 293
                       +N+I +E C R S  +  ++ M +   I +  D  LLIH GII N+ G
Sbjct: 542 TIVVDDLTESLYKNIIGFEQC-RCSNKNFLDYIMLLGCFIKSPTDADLLIHSGIIVNYLG 601

BLAST of Lag0004619 vs. TAIR 10
Match: AT3G50160.1 (Plant protein of unknown function (DUF247) )

HSP 1 Score: 114.8 bits (286), Expect = 1.2e-25
Identity = 107/378 (28.31%), Postives = 167/378 (44.18%), Query Frame = 0

Query: 3   LRSVNPKAYTPQVISIGPFHYEKNNNPMVMEKKGGYVLIFLALAKLSWDQIIEKVMSWEE 62
           L+  + K+Y PQ++SIGP+H+   +   +   K   V + +A AK   +  I+ +   EE
Sbjct: 113 LQENDTKSYMPQIVSIGPYHHGHKHLMPMERHKWRAVNMVMARAKHDIEMYIDAMKELEE 172

Query: 63  RARNSYDEPIKMDRGELVQLLILDGCFVVMYIIGSL--FPE--FRDIDMGFLWR-FNNGI 122
           +AR  Y  PI M+R E +++L+LDG F++    G+   F E  +   D  F  R     I
Sbjct: 173 KARACYQGPINMNRNEFIEMLVLDGVFIIEIFKGTSEGFQEIGYAPNDPVFGMRGLMQSI 232

Query: 123 FKDLLLLENQLPFFLLHALYNL-------------------------------------- 182
            +D+++LENQLP+ +L  L  L                                      
Sbjct: 233 RRDMVMLENQLPWSVLKGLLQLQRPDVLDKVNVQLFQPFFQPLLPTREVLTEEGGLHCLD 292

Query: 183 ------------------CASSQP--------SLRH--VSFI--ELVHSFEISLR----- 242
                               + QP         LR+  V F+  E  H ++I  +     
Sbjct: 293 VLRRGLLQSSGTSDEDMSMVNKQPQQLIHCVTELRNAGVEFMRKETGHFWDIEFKNGYLK 352

Query: 243 ---------------NVIAYEHCHRKSGDHVSNFAMFMHFLINTNKDVSLLIHDGIIHNH 286
                          N+IA+E CH KS   ++++ +FM  LIN+++DVS L H GII N 
Sbjct: 353 IPKLLIHDGTKSLFLNLIAFEQCHIKSSKKITSYIIFMDNLINSSEDVSYLHHYGIIENW 412

BLAST of Lag0004619 vs. TAIR 10
Match: AT3G50150.1 (Plant protein of unknown function (DUF247) )

HSP 1 Score: 110.9 bits (276), Expect = 1.7e-24
Identity = 107/401 (26.68%), Postives = 168/401 (41.90%), Query Frame = 0

Query: 3   LRSVNPKAYTPQVISIGPFHYEKNNNPMVMEKKGGYVLIFLALAKLSWDQIIEKVMSWEE 62
           L+  + K+Y PQ +SIGP+H+ K +   +   K   V + +A  K + +  I+ +   EE
Sbjct: 99  LQENDKKSYLPQTVSIGPYHHGKVHLRPMERHKWRAVNMIMARTKHNIEMYIDAMKELEE 158

Query: 63  RARNSYDEPIKM-DRGELVQLLILDGCFVVMYIIGSLFPEFRDI-----DMGFLWR-FNN 122
            AR  Y  PI M +  E  ++L+LDGCFV+    G++   F+ I     D  F  R   +
Sbjct: 159 EARACYQGPIDMKNSNEFTEMLVLDGCFVLELFKGTI-QGFQKIGYARNDPVFAKRGLMH 218

Query: 123 GIFKDLLLLENQLPFFLLHALYNL------------------------------------ 182
            I +D+++LENQLP F+L  L  L                                    
Sbjct: 219 SIQRDMIMLENQLPLFVLDRLLGLQTGTPNQTGIVAEVAVRFFKTLMPTSEVLTKSERSL 278

Query: 183 ------------------------------------------CASSQPSLRH-------- 242
                                                         Q  L H        
Sbjct: 279 DSQEKSDELGDNGGLHCLDVFHRSLIQSSETTNQGTPYEDMSMVEKQQQLIHCVTELRGA 338

Query: 243 -VSFIE---------------------LVHSFEISL-RNVIAYEHCHRKSGDHVSNFAMF 286
            V+F+                      L+H    SL  N+IA+E CH +S ++++++ +F
Sbjct: 339 GVNFMRKETGQLWDIEFKNGYLKIPKLLIHDGTKSLFSNLIAFEQCHTQSSNNITSYIIF 398

BLAST of Lag0004619 vs. TAIR 10
Match: AT2G36430.1 (Plant protein of unknown function (DUF247) )

HSP 1 Score: 105.9 bits (263), Expect = 5.6e-23
Identity = 100/384 (26.04%), Postives = 156/384 (40.62%), Query Frame = 0

Query: 7   NPKAYTPQVISIGPFHYEKNNNPMVMEKKGGYVLIFLALAK-LSWDQIIEKVMSWEERAR 66
           N + Y P+V+SIGP+H  +    M+ E K  Y+ + L   + L+ +  ++ V + EE AR
Sbjct: 58  NGRCYEPRVVSIGPYHRGQTQLKMIEEHKWRYLNVLLTRTQNLTLEDYMKSVKNVEEVAR 117

Query: 67  NSYDEPIKMDRGELVQLLILDGCFVVMYI--IGSLFP-EFRDIDMGFLWRFNNGIFKDLL 126
             Y E I MD  E  ++++LDGCF++     + +L P E  D  +   W      ++D L
Sbjct: 118 ECYSETIHMDSEEFNEMMVLDGCFLLELFRKVNNLVPFEPNDPLVAMAWVL-PFFYRDFL 177

Query: 127 LLENQLPFFLLHALYNLCA--------SSQPSLRHVSFIELVHSFE-------------- 186
            LENQ+PFF+L  L+NL          +S  SL    F  ++H  E              
Sbjct: 178 CLENQIPFFVLETLFNLTRGDNENETNASLQSLAFAFFNNMMHRTEEDLARFKELRAKHL 237

Query: 187 ------------------------------------------ISLR-------------- 246
                                                     I LR              
Sbjct: 238 LDLLRSSFIPESELHTPPATNPGKEKMPSHIIHSISKLRRAGIKLRELKDAESFLVVRFR 297

Query: 247 --------------------NVIAYEHCHRKSGDHVSNFAMFMHFLINTNKDVSLLIHDG 287
                               N +AYE CH     H + +A  +  L NT KDV  L    
Sbjct: 298 HGTIEMPAITVDDFMSSFLENCVAYEQCHVACSMHFTTYATLLDCLTNTYKDVEYLCDQN 357

BLAST of Lag0004619 vs. TAIR 10
Match: AT3G47200.1 (Plant protein of unknown function (DUF247) )

HSP 1 Score: 105.1 bits (261), Expect = 9.5e-23
Identity = 101/402 (25.12%), Postives = 169/402 (42.04%), Query Frame = 0

Query: 5   SVNPKAYTPQVISIGPFHYEKNNNPMVMEKKGGYVLIFLALAK---LSWDQIIEKVMSWE 64
           ++NPKAY P+V+SIGP+HY + +  M+ + K   + +FL  AK   +  + +++ V+  E
Sbjct: 57  ALNPKAYKPKVVSIGPYHYGEKHLQMIQQHKPRLLQLFLDEAKKKDVEENVLVKAVVDLE 116

Query: 65  ERARNSYDEPIKMDRGELVQLLILDGCFVVM-YIIGSLFPEFRDIDMGFLWRFNNGIFKD 124
           ++ R SY E +K    +L+ +++LDGCF++M ++I S   E  +  +  +    + I  D
Sbjct: 117 DKIRKSYSEELKTGH-DLMFMMVLDGCFILMVFLIMSGNIELSEDPIFSIPWLLSSIQSD 176

Query: 125 LLLLENQLPFFLLHALY------------------------------------------- 184
           LLLLENQ+PFF+L  LY                                           
Sbjct: 177 LLLLENQVPFFVLQTLYVGSKIGVSSDLNRIAFHFFKNPIDKEGSYWEKHRNYKAKHLLD 236

Query: 185 --------NLCASSQPSLRHVSF---------------------------------IELV 244
                   N   S + S  HV                                     L 
Sbjct: 237 LIRETFLPNTSESDKASSPHVQVQLHEGKSGNVPSVDSKAVPLILSAKRLRLQGIKFRLR 296

Query: 245 HSFEISLRNV-------------------------IAYEHCHRKSGDHVSNFAMFMHFLI 291
            S E S+ NV                         +A+E  +  S + ++ + +FM  L+
Sbjct: 297 RSKEDSILNVRLKKNKLQIPQLRFDGFISSFFLNCVAFEQFYTDSSNEITTYIVFMGCLL 356

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
XP_008445209.11.9e-9753.32PREDICTED: UPF0481 protein At3g47200-like [Cucumis melo][more]
KAA0064930.15.5e-9753.06UPF0481 protein [Cucumis melo var. makuwa][more]
XP_004138858.11.9e-9451.41UPF0481 protein At3g47200 [Cucumis sativus] >KGN62944.1 hypothetical protein Csa... [more]
XP_022961896.12.7e-6443.87UPF0481 protein At3g47200-like isoform X2 [Cucurbita moschata][more]
XP_022961897.11.8e-6341.25UPF0481 protein At3g47200-like isoform X3 [Cucurbita moschata] >XP_022961898.1 U... [more]
Match NameE-valueIdentityDescription
Q9SD531.3e-2125.12UPF0481 protein At3g47200 OS=Arabidopsis thaliana OX=3702 GN=At3g47200 PE=2 SV=1[more]
Match NameE-valueIdentityDescription
A0A1S3BD009.1e-9853.32UPF0481 protein At3g47200-like OS=Cucumis melo OX=3656 GN=LOC103488308 PE=4 SV=1[more]
A0A5A7VCL12.6e-9753.06UPF0481 protein OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold82G00248... [more]
A0A0A0LPK89.4e-9551.41Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_2G381700 PE=4 SV=1[more]
A0A6J1HBL71.3e-6443.87UPF0481 protein At3g47200-like isoform X2 OS=Cucurbita moschata OX=3662 GN=LOC11... [more]
A0A6J1HBC38.6e-6441.25UPF0481 protein At3g47200-like isoform X3 OS=Cucurbita moschata OX=3662 GN=LOC11... [more]
Match NameE-valueIdentityDescription
AT4G31980.18.6e-3227.49unknown protein; CONTAINS InterPro DOMAIN/s: Protein of unknown function DUF247,... [more]
AT3G50160.11.2e-2528.31Plant protein of unknown function (DUF247) [more]
AT3G50150.11.7e-2426.68Plant protein of unknown function (DUF247) [more]
AT2G36430.15.6e-2326.04Plant protein of unknown function (DUF247) [more]
AT3G47200.19.5e-2325.12Plant protein of unknown function (DUF247) [more]
InterPro
Analysis Name: InterPro Annotations of Sponge gourd (AG-4) v1
Date Performed: 2022-08-01
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR004158Protein of unknown function DUF247, plantPFAMPF03140DUF247coord: 3..144
e-value: 3.0E-34
score: 119.0
coord: 151..279
e-value: 9.7E-37
score: 127.2
NoneNo IPR availablePANTHERPTHR31170BNAC04G53230D PROTEINcoord: 2..159
NoneNo IPR availablePANTHERPTHR31170:SF13BNAC04G53230D PROTEINcoord: 159..291
NoneNo IPR availablePANTHERPTHR31170:SF13BNAC04G53230D PROTEINcoord: 2..159
NoneNo IPR availablePANTHERPTHR31170BNAC04G53230D PROTEINcoord: 159..291

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Lag0004619.1Lag0004619.1mRNA