Lag0004605 (gene) Sponge gourd (AG‐4) v1

Overview
NameLag0004605
Typegene
OrganismLuffa acutangula (Sponge gourd (AG‐4) v1)
DescriptionF-box domain-containing protein
Locationchr6: 5399289 .. 5401244 (-)
RNA-Seq ExpressionLag0004605
SyntenyLag0004605
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonCDSpolypeptide
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGTATCAGCTGATTTCTTTTTCTTCTCTTGTTCTTGCAGTCACAGAGAGTGAGGATTTTTTCCCTGGGGCACCAATCTATCAAAACCCCAAGGATACAAGTCTCTTTTTGTCCCTTGGTCGTCATGTGGATGTTTATACTCTCCGCAAGAGGTCCCGAGTCAACGCCCCATTTGTGCCTAGTGGAGAGATACTCAAAAAGAGGAAGACAACATCTATTGAAGTTCTTCCAGATGAGTGCCTCTTTGAGATCTTCCGACGCTTGCCTGATAGAGAGACGAGGAGCCTCTGTGCTTGCGTTTCTAAACGCTGGCTCATGCTTCTAAGCAGCATAAGTGGAAATGAATTTTATAGTGCTTCTGAAAATTTGAAGCCTACGAATGTTGTGACTGAAAACGAGGCAGATGACCAAATTGAGAATGATGGACATCTTTCACGAAACTTGGAAGGGAAGAAGGCAACAGATGTAAGACTAGCTGCCATAGCAGTAGGAACTGCTAGTTGTGGTGGCTTGGGCAAGCTATCAATTCGTGGAAACAATGGCTCTCAGGTTACAAACATTGGCCTTAAGGCTGTTGCCCATGGATGTCCTTCCCTTAAAGCTATTTCTTTGTGGAATCTATCTTCCATAGGAGATGAAGGTCTGATTGAGATTGCCAAGGGATGTCAACTCCTAGAGAAGCTTGACTTATGTCAATGTCCTGGAATTTCAAACAAGGCTTTGCTGGAACTTGCCAAACACTGCTCTAATCTGACTGATATAACAATAGAGTCCTGTGCAAACATTGGCAGTGAAAGCCTTCAAGCTATTGGGCAATATTGTTCCAATTTGAAGTCAATTTCGATCAGAGACTGCCCCCTTATCGGGGATCAAGGAATAGCAAGCTTGTTCTCTTCCACCTCTTACACCTTAAACAAGGTGAAGCTCCAGGGTCTTGATGTCACTGATGTGTCGCTTGCTGTCATCGGACATTACGGAAGGGCAGTCACAGACCTTATGCTTACTGGCCTCACAAATGTTACAGAGAGGGGATTTTGGGTCATGGGCAATGGTCATGGTTTGCAAAAGTTGAAATCATTAACACTTACGTCTTGCCATGGTGTGACTGACGTAGGGCTACAATCCATTGGAAAGGGATGCCCAAACTTGAGGAAATTCTGCTTGCGCAAGTGTTCATTTTTATCTGACAATGGCATGGTCTCTTTCGCTCAGGCTGCTGCCTCCATCGAGAACTTACAATTAGAAGAGTGCCACAGGATAACCCAATTGGGATTGTTTGGTACCATCTTAAACTGTGGTGCAAAGCTAAAGGCTCTTTCTCTAGTAAACTGCTTGGGGATCAAGGATTTGAGCCTGAACTTGCCTTCTCTGACTCCATGCAAGTCGCTGCAGTCATTATCCATCTGCAATTGCCCTGGGTTTGGTGATGCAAGCTTAACTCTTCTGAGCAAGCTGTGCCCCCAGCTTCAGCATGTGAATTTATCTGGGCTCAATGGTATTACAGATTCTGGGCTATTGCCATTGCTTAAGAACTGTGAGGCAGGATTGGTGAAGGTCAATTTGAGTGGCTGTGTGAATCTAACCGACAAAGTGATTTCATCCTTGACCAAGCTTCATGGTTGGACTCTTGAAGTGCTCAATCTCGATGGTTGTTCGAAGGTCACAGATTTGAGCTTGGTGGCAATTGCAGAGAACTGTCCATTGCTCAATGATCTCGACATCTCCAAGTGTTGTATCTCTGATTTCGGGATTGCAGCCCTTGCTCAAGCTAATCAGTTCAACCTGCAGATCCTTTCTGTGTTTGGGTGTTCTGCCTTAACTGACAAGAGCTTGTTTGCTCTTGTAAAATTAGGCGACTCCCTTTTGGGCTTGAATCTTCAGCACTGCAACTCAATCAGCACCAGGAGTATTGAGCTGCTTTTGGCTCAACTCCGCCGATGCGACATCCTTTACTGA

mRNA sequence

ATGTATCAGCTGATTTCTTTTTCTTCTCTTGTTCTTGCAGTCACAGAGAGTGAGGATTTTTTCCCTGGGGCACCAATCTATCAAAACCCCAAGGATACAAGTCTCTTTTTGTCCCTTGGTCGTCATGTGGATGTTTATACTCTCCGCAAGAGGTCCCGAGTCAACGCCCCATTTGTGCCTAGTGGAGAGATACTCAAAAAGAGGAAGACAACATCTATTGAAGTTCTTCCAGATGAGTGCCTCTTTGAGATCTTCCGACGCTTGCCTGATAGAGAGACGAGGAGCCTCTGTGCTTGCGTTTCTAAACGCTGGCTCATGCTTCTAAGCAGCATAAGTGGAAATGAATTTTATAGTGCTTCTGAAAATTTGAAGCCTACGAATGTTGTGACTGAAAACGAGGCAGATGACCAAATTGAGAATGATGGACATCTTTCACGAAACTTGGAAGGGAAGAAGGCAACAGATGTAAGACTAGCTGCCATAGCAGTAGGAACTGCTAGTTGTGGTGGCTTGGGCAAGCTATCAATTCGTGGAAACAATGGCTCTCAGGTTACAAACATTGGCCTTAAGGCTGTTGCCCATGGATGTCCTTCCCTTAAAGCTATTTCTTTGTGGAATCTATCTTCCATAGGAGATGAAGGTCTGATTGAGATTGCCAAGGGATGTCAACTCCTAGAGAAGCTTGACTTATGTCAATGTCCTGGAATTTCAAACAAGGCTTTGCTGGAACTTGCCAAACACTGCTCTAATCTGACTGATATAACAATAGAGTCCTGTGCAAACATTGGCAGTGAAAGCCTTCAAGCTATTGGGCAATATTGTTCCAATTTGAAGTCAATTTCGATCAGAGACTGCCCCCTTATCGGGGATCAAGGAATAGCAAGCTTGTTCTCTTCCACCTCTTACACCTTAAACAAGGTGAAGCTCCAGGGTCTTGATGTCACTGATGTGTCGCTTGCTGTCATCGGACATTACGGAAGGGCAGTCACAGACCTTATGCTTACTGGCCTCACAAATGTTACAGAGAGGGGATTTTGGGTCATGGGCAATGGTCATGGTTTGCAAAAGTTGAAATCATTAACACTTACGTCTTGCCATGGTGTGACTGACGTAGGGCTACAATCCATTGGAAAGGGATGCCCAAACTTGAGGAAATTCTGCTTGCGCAAGTGTTCATTTTTATCTGACAATGGCATGGTCTCTTTCGCTCAGGCTGCTGCCTCCATCGAGAACTTACAATTAGAAGAGTGCCACAGGATAACCCAATTGGGATTGTTTGGTACCATCTTAAACTGTGGTGCAAAGCTAAAGGCTCTTTCTCTAGTAAACTGCTTGGGGATCAAGGATTTGAGCCTGAACTTGCCTTCTCTGACTCCATGCAAGTCGCTGCAGTCATTATCCATCTGCAATTGCCCTGGGTTTGGTGATGCAAGCTTAACTCTTCTGAGCAAGCTGTGCCCCCAGCTTCAGCATGTGAATTTATCTGGGCTCAATGGTATTACAGATTCTGGGCTATTGCCATTGCTTAAGAACTGTGAGGCAGGATTGGTGAAGGTCAATTTGAGTGGCTGTGTGAATCTAACCGACAAAGTGATTTCATCCTTGACCAAGCTTCATGGTTGGACTCTTGAAGTGCTCAATCTCGATGGTTGTTCGAAGGTCACAGATTTGAGCTTGGTGGCAATTGCAGAGAACTGTCCATTGCTCAATGATCTCGACATCTCCAAGTGTTGTATCTCTGATTTCGGGATTGCAGCCCTTGCTCAAGCTAATCAGTTCAACCTGCAGATCCTTTCTGTGTTTGGGTGTTCTGCCTTAACTGACAAGAGCTTGTTTGCTCTTGTAAAATTAGGCGACTCCCTTTTGGGCTTGAATCTTCAGCACTGCAACTCAATCAGCACCAGGAGTATTGAGCTGCTTTTGGCTCAACTCCGCCGATGCGACATCCTTTACTGA

Coding sequence (CDS)

ATGTATCAGCTGATTTCTTTTTCTTCTCTTGTTCTTGCAGTCACAGAGAGTGAGGATTTTTTCCCTGGGGCACCAATCTATCAAAACCCCAAGGATACAAGTCTCTTTTTGTCCCTTGGTCGTCATGTGGATGTTTATACTCTCCGCAAGAGGTCCCGAGTCAACGCCCCATTTGTGCCTAGTGGAGAGATACTCAAAAAGAGGAAGACAACATCTATTGAAGTTCTTCCAGATGAGTGCCTCTTTGAGATCTTCCGACGCTTGCCTGATAGAGAGACGAGGAGCCTCTGTGCTTGCGTTTCTAAACGCTGGCTCATGCTTCTAAGCAGCATAAGTGGAAATGAATTTTATAGTGCTTCTGAAAATTTGAAGCCTACGAATGTTGTGACTGAAAACGAGGCAGATGACCAAATTGAGAATGATGGACATCTTTCACGAAACTTGGAAGGGAAGAAGGCAACAGATGTAAGACTAGCTGCCATAGCAGTAGGAACTGCTAGTTGTGGTGGCTTGGGCAAGCTATCAATTCGTGGAAACAATGGCTCTCAGGTTACAAACATTGGCCTTAAGGCTGTTGCCCATGGATGTCCTTCCCTTAAAGCTATTTCTTTGTGGAATCTATCTTCCATAGGAGATGAAGGTCTGATTGAGATTGCCAAGGGATGTCAACTCCTAGAGAAGCTTGACTTATGTCAATGTCCTGGAATTTCAAACAAGGCTTTGCTGGAACTTGCCAAACACTGCTCTAATCTGACTGATATAACAATAGAGTCCTGTGCAAACATTGGCAGTGAAAGCCTTCAAGCTATTGGGCAATATTGTTCCAATTTGAAGTCAATTTCGATCAGAGACTGCCCCCTTATCGGGGATCAAGGAATAGCAAGCTTGTTCTCTTCCACCTCTTACACCTTAAACAAGGTGAAGCTCCAGGGTCTTGATGTCACTGATGTGTCGCTTGCTGTCATCGGACATTACGGAAGGGCAGTCACAGACCTTATGCTTACTGGCCTCACAAATGTTACAGAGAGGGGATTTTGGGTCATGGGCAATGGTCATGGTTTGCAAAAGTTGAAATCATTAACACTTACGTCTTGCCATGGTGTGACTGACGTAGGGCTACAATCCATTGGAAAGGGATGCCCAAACTTGAGGAAATTCTGCTTGCGCAAGTGTTCATTTTTATCTGACAATGGCATGGTCTCTTTCGCTCAGGCTGCTGCCTCCATCGAGAACTTACAATTAGAAGAGTGCCACAGGATAACCCAATTGGGATTGTTTGGTACCATCTTAAACTGTGGTGCAAAGCTAAAGGCTCTTTCTCTAGTAAACTGCTTGGGGATCAAGGATTTGAGCCTGAACTTGCCTTCTCTGACTCCATGCAAGTCGCTGCAGTCATTATCCATCTGCAATTGCCCTGGGTTTGGTGATGCAAGCTTAACTCTTCTGAGCAAGCTGTGCCCCCAGCTTCAGCATGTGAATTTATCTGGGCTCAATGGTATTACAGATTCTGGGCTATTGCCATTGCTTAAGAACTGTGAGGCAGGATTGGTGAAGGTCAATTTGAGTGGCTGTGTGAATCTAACCGACAAAGTGATTTCATCCTTGACCAAGCTTCATGGTTGGACTCTTGAAGTGCTCAATCTCGATGGTTGTTCGAAGGTCACAGATTTGAGCTTGGTGGCAATTGCAGAGAACTGTCCATTGCTCAATGATCTCGACATCTCCAAGTGTTGTATCTCTGATTTCGGGATTGCAGCCCTTGCTCAAGCTAATCAGTTCAACCTGCAGATCCTTTCTGTGTTTGGGTGTTCTGCCTTAACTGACAAGAGCTTGTTTGCTCTTGTAAAATTAGGCGACTCCCTTTTGGGCTTGAATCTTCAGCACTGCAACTCAATCAGCACCAGGAGTATTGAGCTGCTTTTGGCTCAACTCCGCCGATGCGACATCCTTTACTGA

Protein sequence

MYQLISFSSLVLAVTESEDFFPGAPIYQNPKDTSLFLSLGRHVDVYTLRKRSRVNAPFVPSGEILKKRKTTSIEVLPDECLFEIFRRLPDRETRSLCACVSKRWLMLLSSISGNEFYSASENLKPTNVVTENEADDQIENDGHLSRNLEGKKATDVRLAAIAVGTASCGGLGKLSIRGNNGSQVTNIGLKAVAHGCPSLKAISLWNLSSIGDEGLIEIAKGCQLLEKLDLCQCPGISNKALLELAKHCSNLTDITIESCANIGSESLQAIGQYCSNLKSISIRDCPLIGDQGIASLFSSTSYTLNKVKLQGLDVTDVSLAVIGHYGRAVTDLMLTGLTNVTERGFWVMGNGHGLQKLKSLTLTSCHGVTDVGLQSIGKGCPNLRKFCLRKCSFLSDNGMVSFAQAAASIENLQLEECHRITQLGLFGTILNCGAKLKALSLVNCLGIKDLSLNLPSLTPCKSLQSLSICNCPGFGDASLTLLSKLCPQLQHVNLSGLNGITDSGLLPLLKNCEAGLVKVNLSGCVNLTDKVISSLTKLHGWTLEVLNLDGCSKVTDLSLVAIAENCPLLNDLDISKCCISDFGIAALAQANQFNLQILSVFGCSALTDKSLFALVKLGDSLLGLNLQHCNSISTRSIELLLAQLRRCDILY
Homology
BLAST of Lag0004605 vs. NCBI nr
Match: XP_038884618.1 (EIN3-binding F-box protein 1-like [Benincasa hispida])

HSP 1 Score: 1143.3 bits (2956), Expect = 0.0e+00
Identity = 575/640 (89.84%), Postives = 602/640 (94.06%), Query Frame = 0

Query: 14  VTESEDFFPGAPIYQNPKDTSLFLSLGRHVDV-YTLRKRSRVNAPFVPSGEILKKRKTTS 73
           +TESEDFFPGAPIY NPKDTSLFLSLGR VDV Y LRKRSRVN PFVPSGEIL+K K  S
Sbjct: 9   LTESEDFFPGAPIYPNPKDTSLFLSLGRQVDVYYPLRKRSRVNPPFVPSGEILQK-KDAS 68

Query: 74  IEVLPDECLFEIFRRLPDRETRSLCACVSKRWLMLLSSISGNEFYSASENLKPTNVVTEN 133
           IEVLPDECLFEIF+RLPDRETRSLCAC SKRWLMLLSSISGNEFYS S NLKP N+VTEN
Sbjct: 69  IEVLPDECLFEIFKRLPDRETRSLCACTSKRWLMLLSSISGNEFYSGSRNLKPKNIVTEN 128

Query: 134 EADDQIENDGHLSRNLEGKKATDVRLAAIAVGTASCGGLGKLSIR-GNNGSQVTNIGLKA 193
           E D+QIENDG+LSRNLEGKKATDVRLAAIAVGTASCGGLGKLSIR GN+G +VTN+GLKA
Sbjct: 129 EEDNQIENDGYLSRNLEGKKATDVRLAAIAVGTASCGGLGKLSIRGGNHGFEVTNLGLKA 188

Query: 194 VAHGCPSLKAISLWNLSSIGDEGLIEIAKGCQLLEKLDLCQCPGISNKALLELAKHCSNL 253
           VAHGCPSLKAISLWNLSSIGDEGLIEIAKGCQLLEKLDLC CPGISNKALL+LAK+C NL
Sbjct: 189 VAHGCPSLKAISLWNLSSIGDEGLIEIAKGCQLLEKLDLCHCPGISNKALLQLAKNCPNL 248

Query: 254 TDITIESCANIGSESLQAIGQYCSNLKSISIRDCPLIGDQGIASLFSSTSYTLNKVKLQG 313
           TDITIESC  IG+ESLQAIG+ C NLKSISIRDCPLIGDQGI SLFSSTSYTLNKVKLQG
Sbjct: 249 TDITIESCVKIGNESLQAIGKSCLNLKSISIRDCPLIGDQGIVSLFSSTSYTLNKVKLQG 308

Query: 314 LDVTDVSLAVIGHYGRAVTDLMLTGLTNVTERGFWVMGNGHGLQKLKSLTLTSCHGVTDV 373
           L+VTDVSLAVIGHYGRAVTDLMLTGLTNV+ERGFW MGNGHGLQKL+S TL SCHGVTDV
Sbjct: 309 LNVTDVSLAVIGHYGRAVTDLMLTGLTNVSERGFWAMGNGHGLQKLRSFTLNSCHGVTDV 368

Query: 374 GLQSIGKGCPNLRKFCLRKCSFLSDNGMVSFAQAAASIENLQLEECHRITQLGLFGTILN 433
           GLQSIGKGCPNLRKFCLRKCSFLSDNGMVSFA+AA SIENLQLEECHRITQLGLFGTILN
Sbjct: 369 GLQSIGKGCPNLRKFCLRKCSFLSDNGMVSFAEAATSIENLQLEECHRITQLGLFGTILN 428

Query: 434 CGAKLKALSLVNCLGIKDLSLNLPSLTPCKSLQSLSICNCPGFGDASLTLLSKLCPQLQH 493
           CGAKLKALSLVNCLGIKDLSLNLPSL+ CK LQSLS+CNCPGFG+ASLTLLSKLCPQLQH
Sbjct: 429 CGAKLKALSLVNCLGIKDLSLNLPSLSSCKLLQSLSVCNCPGFGNASLTLLSKLCPQLQH 488

Query: 494 VNLSGLNGITDSGLLPLLKNCEAGLVKVNLSGCVNLTDKVISSLTKLHGWTLEVLNLDGC 553
           V LSGLNGITDSGLLPL  NC+AGLVKVNLSGCVNLTDKV+SSLTKLHGWTLE+LNLDGC
Sbjct: 489 VELSGLNGITDSGLLPLFMNCKAGLVKVNLSGCVNLTDKVVSSLTKLHGWTLELLNLDGC 548

Query: 554 SKVTDLSLVAIAENCPLLNDLDISKCCISDFGIAALAQANQFNLQILSVFGCSALTDKSL 613
            KVTD SLV IAENCPLLNDLD+SKCCI+DFG+A LAQANQFNLQILSVFGCSALTD+SL
Sbjct: 549 LKVTDSSLVEIAENCPLLNDLDVSKCCITDFGVATLAQANQFNLQILSVFGCSALTDQSL 608

Query: 614 FALVKLGDSLLGLNLQHCNSISTRSIELLLAQLRRCDILY 652
            A VKLGDSLLGLNLQHCNSISTRSIELLLAQL RCDILY
Sbjct: 609 LAFVKLGDSLLGLNLQHCNSISTRSIELLLAQLHRCDILY 647

BLAST of Lag0004605 vs. NCBI nr
Match: KAA0064924.1 (EIN3-binding F-box protein 1 [Cucumis melo var. makuwa])

HSP 1 Score: 1140.6 bits (2949), Expect = 0.0e+00
Identity = 582/653 (89.13%), Postives = 614/653 (94.03%), Query Frame = 0

Query: 1   MYQLISFSSLVLAVTESEDFFPGAPIYQNPKDTSLFLSLGRHVDV-YTLRKRSRVNAPFV 60
           MYQLI + SL LAV ESEDFFP APIY NPKDTSLFLSLG HVDV Y LRKRSRV+AP+V
Sbjct: 23  MYQLI-YCSLDLAVKESEDFFP-APIYPNPKDTSLFLSLGPHVDVYYPLRKRSRVSAPYV 82

Query: 61  PSGEILKKRKTTSIEVLPDECLFEIFRRLPDRETRSLCACVSKRWLMLLSSISGNEFYSA 120
           PSGEILKK K  SIEVLPDECLFEIF+RLPDRETRSLCACVSKRWLMLLSSISGNEFYSA
Sbjct: 83  PSGEILKK-KEASIEVLPDECLFEIFKRLPDRETRSLCACVSKRWLMLLSSISGNEFYSA 142

Query: 121 SENLKPTNVVTENEADDQIENDGHLSRNLEGKKATDVRLAAIAVGTASCGGLGKLSIR-G 180
           SENLKP  VVTEN  D+QI N+G LSRNLEGKKATDVRLAAIAVGTASCGGLGKLSIR G
Sbjct: 143 SENLKPKIVVTENLEDNQITNEGCLSRNLEGKKATDVRLAAIAVGTASCGGLGKLSIRGG 202

Query: 181 NNGSQVTNIGLKAVAHGCPSLKAISLWNLSSIGDEGLIEIAKGCQLLEKLDLCQCPGISN 240
           N+G++VTN+GLKAVAHGCP+LKAISLWNLSSIGDEG+IEIAKGCQLLEKLDL QCPGISN
Sbjct: 203 NHGTEVTNLGLKAVAHGCPALKAISLWNLSSIGDEGMIEIAKGCQLLEKLDLSQCPGISN 262

Query: 241 KALLELAKHCSNLTDITIESCANIGSESLQAIGQYCSNLKSISIRDCPLIGDQGIASLFS 300
           KALLELAKHC NLTDITIE+CANIG+ES+QAIGQYCSNLKSISIRDCPLIGDQGI+SLFS
Sbjct: 263 KALLELAKHCPNLTDITIEACANIGNESIQAIGQYCSNLKSISIRDCPLIGDQGISSLFS 322

Query: 301 STSYTLNKVKLQGLDVTDVSLAVIGHYGRAVTDLMLTGLTNVTERGFWVMGNGHGLQKLK 360
           STSYTLNK KLQGL+VTDVSLAVIGHYGRA+TDL LTGLTNV+ERGFW MGNGHGLQKL+
Sbjct: 323 STSYTLNKAKLQGLNVTDVSLAVIGHYGRAITDLTLTGLTNVSERGFWAMGNGHGLQKLR 382

Query: 361 SLTLTSCHGVTDVGLQSIGKGCPNLRKFCLRKCSFLSDNGMVSFAQAAASIENLQLEECH 420
           S TL+SCHGVTDVGLQSIGKGCPN++KFCLRKCSFLSDNGMVSF QAA SIENLQLEECH
Sbjct: 383 SFTLSSCHGVTDVGLQSIGKGCPNMKKFCLRKCSFLSDNGMVSFVQAATSIENLQLEECH 442

Query: 421 RITQLGLFGTILNCGAKLKALSLVNCLGIKDLSLNLPSLTPCKSLQSLSICNCPGFGDAS 480
           RITQLGLFGTILNCGAKLKALSLVNCLGIKDLSLNLPSL+ CKSLQSLSI NCPGFG+AS
Sbjct: 443 RITQLGLFGTILNCGAKLKALSLVNCLGIKDLSLNLPSLSSCKSLQSLSIRNCPGFGNAS 502

Query: 481 LTLLSKLCPQLQHVNLSGLNGITDSGLLPLLKNCEAGLVKVNLSGCVNLTDKVISSLTKL 540
           LTLLSKLCPQLQHV  SGLNGI DSGLLPL  NC+AGLV VNLSGCVNLTDKVISSLTKL
Sbjct: 503 LTLLSKLCPQLQHVEFSGLNGIRDSGLLPLFMNCKAGLVNVNLSGCVNLTDKVISSLTKL 562

Query: 541 HGWTLEVLNLDGCSKVTDLSLVAIAENCPLLNDLDISKCCISDFGIAALAQANQFNLQIL 600
           HGWTLE+LNLDGC KVTD SLVAIAENCPLLNDLD+SKCCI+DFG+AALAQANQFNLQ+L
Sbjct: 563 HGWTLELLNLDGCLKVTDSSLVAIAENCPLLNDLDVSKCCITDFGVAALAQANQFNLQLL 622

Query: 601 SVFGCSALTDKSLFALVKLGDSLLGLNLQHCNSISTRSIELLLAQLRRCDILY 652
           SVFGCSALTD+SL ALVKLGDSLLGLNLQHCNSISTRSIELLLAQL RCDILY
Sbjct: 623 SVFGCSALTDQSLLALVKLGDSLLGLNLQHCNSISTRSIELLLAQLHRCDILY 672

BLAST of Lag0004605 vs. NCBI nr
Match: XP_023537957.1 (EIN3-binding F-box protein 1-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 1139.8 bits (2947), Expect = 0.0e+00
Identity = 573/640 (89.53%), Postives = 607/640 (94.84%), Query Frame = 0

Query: 14  VTESEDFFPGAPIYQNPKDTSLFLSLGRHVDV-YTLRKRSRVNAPFVPSGEILKKRKTTS 73
           +TESEDFF GAPIYQNPKDTSLFLSLGR VDV Y LRKRSR    FVPSGEILKK+K TS
Sbjct: 9   LTESEDFFSGAPIYQNPKDTSLFLSLGRQVDVYYPLRKRSRATESFVPSGEILKKKK-TS 68

Query: 74  IEVLPDECLFEIFRRLPDRETRSLCACVSKRWLMLLSSISGNEFYSASENLKPTNVVTEN 133
           IE+LPDECLFE+FRRLPDRETRSLCACVSKRWLMLLS+ISGNEFYSASENL+P NV T N
Sbjct: 69  IEILPDECLFEVFRRLPDRETRSLCACVSKRWLMLLSNISGNEFYSASENLRPENVATVN 128

Query: 134 EADDQIENDGHLSRNLEGKKATDVRLAAIAVGTASCGGLGKLSIRGN-NGSQVTNIGLKA 193
           E D+++ENDGHLSRNLEG+KATD+RLAAIAVGTASCGGLGKLSIRG  +GS+V+NIGLKA
Sbjct: 129 ETDNKVENDGHLSRNLEGEKATDIRLAAIAVGTASCGGLGKLSIRGGIHGSKVSNIGLKA 188

Query: 194 VAHGCPSLKAISLWNLSSIGDEGLIEIAKGCQLLEKLDLCQCPGISNKALLELAKHCSNL 253
           VA GCPSLKAISLWNLSSIGDEGLIEIAKGCQ LEKLDLCQCPGISNKALLELAK+C NL
Sbjct: 189 VARGCPSLKAISLWNLSSIGDEGLIEIAKGCQFLEKLDLCQCPGISNKALLELAKNCPNL 248

Query: 254 TDITIESCANIGSESLQAIGQYCSNLKSISIRDCPLIGDQGIASLFSSTSYTLNKVKLQG 313
           TDITIESCANIG+E LQA+GQYCSNLKSISIRDCPLIGDQG+ASLF ST+ TLNKVKLQG
Sbjct: 249 TDITIESCANIGNEGLQALGQYCSNLKSISIRDCPLIGDQGLASLFLSTADTLNKVKLQG 308

Query: 314 LDVTDVSLAVIGHYGRAVTDLMLTGLTNVTERGFWVMGNGHGLQKLKSLTLTSCHGVTDV 373
           L+VTD+SLAVIGHYGRAVTDLMLTGLTNVTE+GFWVMGNGHGLQKLKS TL SCHGVTDV
Sbjct: 309 LNVTDMSLAVIGHYGRAVTDLMLTGLTNVTEKGFWVMGNGHGLQKLKSFTLASCHGVTDV 368

Query: 374 GLQSIGKGCPNLRKFCLRKCSFLSDNGMVSFAQAAASIENLQLEECHRITQLGLFGTILN 433
           GLQSI KGCPNL+KFCLRKCSFLSD GMVSF QAA SIENLQLEECHRITQLGLFGTILN
Sbjct: 369 GLQSIAKGCPNLKKFCLRKCSFLSDKGMVSFTQAALSIENLQLEECHRITQLGLFGTILN 428

Query: 434 CGAKLKALSLVNCLGIKDLSLNLPSLTPCKSLQSLSICNCPGFGDASLTLLSKLCPQLQH 493
           CGAKLKALSLVNCLGIKDLSLNLPS TPCKSLQSLSICNCPGFG+ SLTLLSKLCPQLQH
Sbjct: 429 CGAKLKALSLVNCLGIKDLSLNLPSTTPCKSLQSLSICNCPGFGNVSLTLLSKLCPQLQH 488

Query: 494 VNLSGLNGITDSGLLPLLKNCEAGLVKVNLSGCVNLTDKVISSLTKLHGWTLEVLNLDGC 553
           VNLSGL+GI+DSGLLPLLKNCEAGLV VNLSGCVNLTDKVISSLTKLHGWTLEVLN+DGC
Sbjct: 489 VNLSGLSGISDSGLLPLLKNCEAGLVNVNLSGCVNLTDKVISSLTKLHGWTLEVLNVDGC 548

Query: 554 SKVTDLSLVAIAENCPLLNDLDISKCCISDFGIAALAQANQFNLQILSVFGCSALTDKSL 613
           SKVTD SLVAI+ENCPLLNDLD+SKC I+DFG+AALAQANQFNLQILSVFGCSALTDKSL
Sbjct: 549 SKVTDSSLVAISENCPLLNDLDVSKCGITDFGVAALAQANQFNLQILSVFGCSALTDKSL 608

Query: 614 FALVKLGDSLLGLNLQHCNSISTRSIELLLAQLRRCDILY 652
           FAL+KLGDSLLGLNL++CNSISTRSIELLLAQL RCDILY
Sbjct: 609 FALMKLGDSLLGLNLRNCNSISTRSIELLLAQLHRCDILY 647

BLAST of Lag0004605 vs. NCBI nr
Match: XP_023002260.1 (EIN3-binding F-box protein 1-like [Cucurbita maxima])

HSP 1 Score: 1135.2 bits (2935), Expect = 0.0e+00
Identity = 572/640 (89.38%), Postives = 605/640 (94.53%), Query Frame = 0

Query: 14  VTESEDFFPGAPIYQNPKDTSLFLSLGRHVDV-YTLRKRSRVNAPFVPSGEILKKRKTTS 73
           +TESEDFF GAPIYQNPKDTSLFLSLGR VDV Y LRKRSR    FVPSGEILKK+K TS
Sbjct: 9   LTESEDFFSGAPIYQNPKDTSLFLSLGRRVDVYYPLRKRSRATESFVPSGEILKKKK-TS 68

Query: 74  IEVLPDECLFEIFRRLPDRETRSLCACVSKRWLMLLSSISGNEFYSASENLKPTNVVTEN 133
           IE+LPDECLFE+FRRLPDRETRSLCACVSKRWLMLLSSISGNEFYSASENL+P NVV  N
Sbjct: 69  IEILPDECLFEVFRRLPDRETRSLCACVSKRWLMLLSSISGNEFYSASENLRPENVVAVN 128

Query: 134 EADDQIENDGHLSRNLEGKKATDVRLAAIAVGTASCGGLGKLSIRGN-NGSQVTNIGLKA 193
           E D+++ENDGHLSRNLEG+KATD+RLAAIAVGTASCGGLGKLSIRG  +GS+V+NIGLKA
Sbjct: 129 ETDNKVENDGHLSRNLEGEKATDIRLAAIAVGTASCGGLGKLSIRGGIHGSKVSNIGLKA 188

Query: 194 VAHGCPSLKAISLWNLSSIGDEGLIEIAKGCQLLEKLDLCQCPGISNKALLELAKHCSNL 253
           VA GCPSLKAISLWNLSSIGDEGLIEIAKGCQ LEKLDLCQCPGISNKALLELAK+C NL
Sbjct: 189 VARGCPSLKAISLWNLSSIGDEGLIEIAKGCQFLEKLDLCQCPGISNKALLELAKNCPNL 248

Query: 254 TDITIESCANIGSESLQAIGQYCSNLKSISIRDCPLIGDQGIASLFSSTSYTLNKVKLQG 313
           TDITIESCANIG+E LQA+GQYCSNLKSISIRDCPLIGDQG+ASLF ST+ TL+KVKLQG
Sbjct: 249 TDITIESCANIGNEGLQALGQYCSNLKSISIRDCPLIGDQGLASLFFSTADTLSKVKLQG 308

Query: 314 LDVTDVSLAVIGHYGRAVTDLMLTGLTNVTERGFWVMGNGHGLQKLKSLTLTSCHGVTDV 373
           L+VTD+SLAVIGHYGR VTDLMLTGLTNVTE+GFWVMG+GHGLQKLKS TL SCHGVTDV
Sbjct: 309 LNVTDMSLAVIGHYGREVTDLMLTGLTNVTEKGFWVMGSGHGLQKLKSFTLASCHGVTDV 368

Query: 374 GLQSIGKGCPNLRKFCLRKCSFLSDNGMVSFAQAAASIENLQLEECHRITQLGLFGTILN 433
           GLQSI KGCPNL+KFCLRKCSFLSD GMVSF QAA SIENLQLEECHRITQLGLFGTILN
Sbjct: 369 GLQSIAKGCPNLKKFCLRKCSFLSDKGMVSFTQAALSIENLQLEECHRITQLGLFGTILN 428

Query: 434 CGAKLKALSLVNCLGIKDLSLNLPSLTPCKSLQSLSICNCPGFGDASLTLLSKLCPQLQH 493
           CGAKLKALSLVNCLGIKDLSLNLPS TPCKSLQSLSICNCPGFG+ SLTLLSKLCPQLQH
Sbjct: 429 CGAKLKALSLVNCLGIKDLSLNLPSTTPCKSLQSLSICNCPGFGNVSLTLLSKLCPQLQH 488

Query: 494 VNLSGLNGITDSGLLPLLKNCEAGLVKVNLSGCVNLTDKVISSLTKLHGWTLEVLNLDGC 553
           VNLSGL+GI+DSGLLPLLKNCEAGLV VNLSGCVNLTDKVISSLTKLHGWTLEVLN+DGC
Sbjct: 489 VNLSGLSGISDSGLLPLLKNCEAGLVNVNLSGCVNLTDKVISSLTKLHGWTLEVLNVDGC 548

Query: 554 SKVTDLSLVAIAENCPLLNDLDISKCCISDFGIAALAQANQFNLQILSVFGCSALTDKSL 613
           SKVTD SLVAIAENCPLLNDLD+SKC I+DFG+AALAQANQFNLQILSVFGCSALTDKSL
Sbjct: 549 SKVTDSSLVAIAENCPLLNDLDVSKCRITDFGVAALAQANQFNLQILSVFGCSALTDKSL 608

Query: 614 FALVKLGDSLLGLNLQHCNSISTRSIELLLAQLRRCDILY 652
           FAL KLGDSLLGLNL++CNSISTRSIELLLAQL RCDILY
Sbjct: 609 FALFKLGDSLLGLNLRNCNSISTRSIELLLAQLHRCDILY 647

BLAST of Lag0004605 vs. NCBI nr
Match: XP_004138725.1 (EIN3-binding F-box protein 1 [Cucumis sativus] >KGN62950.1 hypothetical protein Csa_022599 [Cucumis sativus])

HSP 1 Score: 1133.6 bits (2931), Expect = 0.0e+00
Identity = 572/640 (89.38%), Postives = 603/640 (94.22%), Query Frame = 0

Query: 14  VTESEDFFPGAPIYQNPKDTSLFLSLGRHVDV-YTLRKRSRVNAPFVPSGEILKKRKTTS 73
           +TESEDFFPGAPIY NPKDTSLFLSLGR VDV Y LRKRSRV+APFVPSGEILKK    S
Sbjct: 9   LTESEDFFPGAPIYPNPKDTSLFLSLGRQVDVYYPLRKRSRVSAPFVPSGEILKKE--AS 68

Query: 74  IEVLPDECLFEIFRRLPDRETRSLCACVSKRWLMLLSSISGNEFYSASENLKPTNVVTEN 133
           IEVLPDECLFEIF+RLP+RETRSLCACVSKRWLMLLSSISGNEFY ASENLKP NVVTEN
Sbjct: 69  IEVLPDECLFEIFKRLPERETRSLCACVSKRWLMLLSSISGNEFYGASENLKPKNVVTEN 128

Query: 134 EADDQIENDGHLSRNLEGKKATDVRLAAIAVGTASCGGLGKLSIR-GNNGSQVTNIGLKA 193
             D+Q  N+G LSRNLEGKKATDVRLAAIAVGTASCGGLGKLSIR GN+GS+VTN+GLKA
Sbjct: 129 LEDNQTTNEGCLSRNLEGKKATDVRLAAIAVGTASCGGLGKLSIRGGNHGSEVTNLGLKA 188

Query: 194 VAHGCPSLKAISLWNLSSIGDEGLIEIAKGCQLLEKLDLCQCPGISNKALLELAKHCSNL 253
           VAHGCP LKAISLWNLSSIGDEGLIEIAKGCQLLEKLDL QCPGISNKALLELAK+C NL
Sbjct: 189 VAHGCPGLKAISLWNLSSIGDEGLIEIAKGCQLLEKLDLSQCPGISNKALLELAKNCPNL 248

Query: 254 TDITIESCANIGSESLQAIGQYCSNLKSISIRDCPLIGDQGIASLFSSTSYTLNKVKLQG 313
           TDIT+E+CANIG+ES+QAIGQYCSNLKSISIRDCPLIGDQGI+SLFSSTSYTLNK KLQG
Sbjct: 249 TDITVEACANIGNESVQAIGQYCSNLKSISIRDCPLIGDQGISSLFSSTSYTLNKAKLQG 308

Query: 314 LDVTDVSLAVIGHYGRAVTDLMLTGLTNVTERGFWVMGNGHGLQKLKSLTLTSCHGVTDV 373
           L+VTDVSLAVIGHYGRA+TDL LTGLTNV+ERGFW MGNGHGLQKL+S TL+SCHGVTDV
Sbjct: 309 LNVTDVSLAVIGHYGRAITDLTLTGLTNVSERGFWAMGNGHGLQKLRSFTLSSCHGVTDV 368

Query: 374 GLQSIGKGCPNLRKFCLRKCSFLSDNGMVSFAQAAASIENLQLEECHRITQLGLFGTILN 433
           GLQSIGKGCPNL+KFCL KCSFLSDNGMVSF QAA SIENLQLEECHRITQLGLFGTILN
Sbjct: 369 GLQSIGKGCPNLKKFCLHKCSFLSDNGMVSFVQAATSIENLQLEECHRITQLGLFGTILN 428

Query: 434 CGAKLKALSLVNCLGIKDLSLNLPSLTPCKSLQSLSICNCPGFGDASLTLLSKLCPQLQH 493
           CGAKLKALSLVNCLGIKDLSLNLPSL+ CKSLQSLSI NCPGFG+ASLTLLSKLCPQLQH
Sbjct: 429 CGAKLKALSLVNCLGIKDLSLNLPSLSSCKSLQSLSIRNCPGFGNASLTLLSKLCPQLQH 488

Query: 494 VNLSGLNGITDSGLLPLLKNCEAGLVKVNLSGCVNLTDKVISSLTKLHGWTLEVLNLDGC 553
           V  SGLN ITDSGLLPL  NC+AGLVKVNLSGCVNLTDKVISSLTKLHGWTLE+LNLDGC
Sbjct: 489 VEFSGLNAITDSGLLPLFMNCKAGLVKVNLSGCVNLTDKVISSLTKLHGWTLELLNLDGC 548

Query: 554 SKVTDLSLVAIAENCPLLNDLDISKCCISDFGIAALAQANQFNLQILSVFGCSALTDKSL 613
            KVTD SLVAIAENCPLLNDLD+SKCCI+DFG+AALAQANQFNLQ+LSV+GCSALTD+SL
Sbjct: 549 LKVTDSSLVAIAENCPLLNDLDVSKCCITDFGVAALAQANQFNLQLLSVYGCSALTDQSL 608

Query: 614 FALVKLGDSLLGLNLQHCNSISTRSIELLLAQLRRCDILY 652
            ALVKLGDSLLGLNLQHCNSISTRSIELLLAQL RCDILY
Sbjct: 609 LALVKLGDSLLGLNLQHCNSISTRSIELLLAQLHRCDILY 646

BLAST of Lag0004605 vs. ExPASy Swiss-Prot
Match: Q9SKK0 (EIN3-binding F-box protein 1 OS=Arabidopsis thaliana OX=3702 GN=EBF1 PE=1 SV=1)

HSP 1 Score: 740.3 bits (1910), Expect = 1.8e-212
Identity = 379/643 (58.94%), Postives = 492/643 (76.52%), Query Frame = 0

Query: 9   SLVLAVTESEDFFPGAPIYQNPKDTSLFLSLGRHVDVY-TLRKRSRVNAPFVPSGEILKK 68
           S + +     DF+    IY NPKD SL LSLG   DVY    KRSRV AP + S     +
Sbjct: 2   SQIFSFAGENDFYRRGAIYPNPKDASLLLSLGSFADVYFPPSKRSRVVAPTIFSA---FE 61

Query: 69  RKTTSIEVLPDECLFEIFRRLPDRETRSLCACVSKRWLMLLSSISGNEFYSASENLKPTN 128
           +K  SI+VLPDECLFEIFRRL   + RS CA VSK+WL L+SSI   E    S+      
Sbjct: 62  KKPVSIDVLPDECLFEIFRRLSGPQERSACAFVSKQWLTLVSSIRQKEIDVPSK------ 121

Query: 129 VVTENEADDQIENDGHLSRNLEGKKATDVRLAAIAVGTASCGGLGKLSIRGNNGSQVTNI 188
            +TE    D  + +G LSR+L+GKKATDVRLAAIAVGTA  GGLGKLSIRG+N ++V+++
Sbjct: 122 -ITE----DGDDCEGCLSRSLDGKKATDVRLAAIAVGTAGRGGLGKLSIRGSNSAKVSDL 181

Query: 189 GLKAVAHGCPSLKAISLWNLSSIGDEGLIEIAKGCQLLEKLDLCQCPGISNKALLELAKH 248
           GL+++   CPSL ++SLWN+S+I D GL+EIA+GC  LEKL+L +C  I++K L+ +AK 
Sbjct: 182 GLRSIGRSCPSLGSLSLWNVSTITDNGLLEIAEGCAQLEKLELNRCSTITDKGLVAIAKS 241

Query: 249 CSNLTDITIESCANIGSESLQAIGQYCSNLKSISIRDCPLIGDQGIASLFSSTSYTLNKV 308
           C NLT++T+E+C+ IG E L AI + CS LKS+SI++CPL+ DQGIASL S+T+ +L K+
Sbjct: 242 CPNLTELTLEACSRIGDEGLLAIARSCSKLKSVSIKNCPLVRDQGIASLLSNTTCSLAKL 301

Query: 309 KLQGLDVTDVSLAVIGHYGRAVTDLMLTGLTNVTERGFWVMGNGHGLQKLKSLTLTSCHG 368
           KLQ L+VTDVSLAV+GHYG ++TDL+L GL++V+E+GFWVMGNG GLQKL SLT+T+C G
Sbjct: 302 KLQMLNVTDVSLAVVGHYGLSITDLVLAGLSHVSEKGFWVMGNGVGLQKLNSLTITACQG 361

Query: 369 VTDVGLQSIGKGCPNLRKFCLRKCSFLSDNGMVSFAQAAASIENLQLEECHRITQLGLFG 428
           VTD+GL+S+GKGCPN++K  + K   LSDNG+VSFA+A+ S+E+LQLEECHR+TQ G FG
Sbjct: 362 VTDMGLESVGKGCPNMKKAIISKSPLLSDNGLVSFAKASLSLESLQLEECHRVTQFGFFG 421

Query: 429 TILNCGAKLKALSLVNCLGIKDLSLNLPSLTPCKSLQSLSICNCPGFGDASLTLLSKLCP 488
           ++LNCG KLKA SLVNCL I+DL+  LP+ + C +L+SLSI NCPGFGDA+L  + KLCP
Sbjct: 422 SLLNCGEKLKAFSLVNCLSIRDLTTGLPASSHCSALRSLSIRNCPGFGDANLAAIGKLCP 481

Query: 489 QLQHVNLSGLNGITDSGLLPLLKNCEAGLVKVNLSGCVNLTDKVISSLTKLHGWTLEVLN 548
           QL+ ++L GL GIT+SG L L+   ++ LVK+N SGC NLTD+VIS++T  +GWTLEVLN
Sbjct: 482 QLEDIDLCGLKGITESGFLHLI---QSSLVKINFSGCSNLTDRVISAITARNGWTLEVLN 541

Query: 549 LDGCSKVTDLSLVAIAENCPLLNDLDISKCCISDFGIAALAQANQFNLQILSVFGCSALT 608
           +DGCS +TD SLV+IA NC +L+DLDISKC ISD GI ALA +++  LQILSV GCS +T
Sbjct: 542 IDGCSNITDASLVSIAANCQILSDLDISKCAISDSGIQALASSDKLKLQILSVAGCSMVT 601

Query: 609 DKSLFALVKLGDSLLGLNLQHCNSISTRSIELLLAQLRRCDIL 651
           DKSL A+V LG +LLGLNLQ C SIS  +++ L+ +L +CDIL
Sbjct: 602 DKSLPAIVGLGSTLLGLNLQQCRSISNSTVDFLVERLYKCDIL 627

BLAST of Lag0004605 vs. ExPASy Swiss-Prot
Match: Q708Y0 (EIN3-binding F-box protein 2 OS=Arabidopsis thaliana OX=3702 GN=EBF2 PE=1 SV=1)

HSP 1 Score: 673.3 bits (1736), Expect = 2.7e-192
Identity = 355/623 (56.98%), Postives = 454/623 (72.87%), Query Frame = 0

Query: 34  SLFLSLGRHVDV-YTLRKRSRVNAPFVPSGEILKKRKTTSIEVLPDECLFEIFRRLPDRE 93
           S++LS G    V Y  RKR RV A    SG    + K TSI+VLP+ECLFEI RRLP  +
Sbjct: 18  SMYLSPGSCPGVYYPARKRLRVAATSFYSG---FEEKQTSIDVLPEECLFEILRRLPSGQ 77

Query: 94  TRSLCACVSKRWLMLLSSISGNEFYSASENLKPTNVVTENEADDQIENDGHLSRNLEGKK 153
            RS CACVSK WL LLSSIS +E             V E+   D  E +G LSR+LEGKK
Sbjct: 78  ERSACACVSKHWLNLLSSISRSE-------------VNESSVQDVEEGEGFLSRSLEGKK 137

Query: 154 ATDVRLAAIAVGTASCGGLGKLSIRGNN-GSQVTNIGLKAVAHGCPSLKAISLWNLSSIG 213
           ATD+RLAAIAVGT+S GGLGKL IRG+   S+VT++GL AVAHGCPSL+ +SLWNL ++ 
Sbjct: 138 ATDLRLAAIAVGTSSRGGLGKLQIRGSGFESKVTDVGLGAVAHGCPSLRIVSLWNLPAVS 197

Query: 214 DEGLIEIAKGCQLLEKLDLCQCPGISNKALLELAKHCSNLTDITIESCANIGSESLQAIG 273
           D GL EIA+ C ++EKLDL +CPGI++  L+ +A++C NL+D+TI+SC+ +G+E L+AI 
Sbjct: 198 DLGLSEIARSCPMIEKLDLSRCPGITDSGLVAIAENCVNLSDLTIDSCSGVGNEGLRAIA 257

Query: 274 QYCSNLKSISIRDCPLIGDQGIASLFSSTSYTLNKVKLQGLDVTDVSLAVIGHYGRAVTD 333
           + C NL+SISIR CP IGDQG+A L +     L KVKLQ L+V+ +SLAVIGHYG AVTD
Sbjct: 258 RRCVNLRSISIRSCPRIGDQGVAFLLAQAGSYLTKVKLQMLNVSGLSLAVIGHYGAAVTD 317

Query: 334 LMLTGLTNVTERGFWVMGNGHGLQKLKSLTLTSCHGVTDVGLQSIGKGCPNLRKFCLRKC 393
           L+L GL  V E+GFWVMGN  GL+KLKSL++ SC G+TDVGL+++G GCP+L+   L KC
Sbjct: 318 LVLHGLQGVNEKGFWVMGNAKGLKKLKSLSVMSCRGMTDVGLEAVGNGCPDLKHVSLNKC 377

Query: 394 SFLSDNGMVSFAQAAASIENLQLEECHRITQLGLFGTILNCGAKLKALSLVNCLGIKDLS 453
             +S  G+V+ A++A S+E+L+LEECHRI Q GL G ++NCG+KLKA SL NCLGI D +
Sbjct: 378 LLVSGKGLVALAKSALSLESLKLEECHRINQFGLMGFLMNCGSKLKAFSLANCLGISDFN 437

Query: 454 --LNLPSLTPCKSLQSLSICNCPGFGDASLTLLSKLCPQLQHVNLSGLNGITDSGLLPLL 513
              +LPS   C SL+SLSI  CPGFGDASL  L K C QLQ V L GLNG+TD+G+  LL
Sbjct: 438 SESSLPS-PSCSSLRSLSIRCCPGFGDASLAFLGKFCHQLQDVELCGLNGVTDAGVRELL 497

Query: 514 KNCEAGLVKVNLSGCVNLTDKVISSLTKLHGWTLEVLNLDGCSKVTDLSLVAIAENCPLL 573
           ++   GLVKVNLS C+N++D  +S+++  HG TLE LNLDGC  +T+ SLVA+A+NC  +
Sbjct: 498 QSNNVGLVKVNLSECINVSDNTVSAISVCHGRTLESLNLDGCKNITNASLVAVAKNCYSV 557

Query: 574 NDLDISKCCISDFGIAALAQA-NQFNLQILSVFGCSALTDKSLFALVKLGDSLLGLNLQH 633
           NDLDIS   +SD GI ALA + N  NLQ+LS+ GCS++TDKS   + KLG +LLGLN+Q 
Sbjct: 558 NDLDISNTLVSDHGIKALASSPNHLNLQVLSIGGCSSITDKSKACIQKLGRTLLGLNIQR 617

Query: 634 CNSISTRSIELLLAQLRRCDILY 652
           C  IS+ +++ LL  L RCDILY
Sbjct: 618 CGRISSSTVDTLLENLWRCDILY 623

BLAST of Lag0004605 vs. ExPASy Swiss-Prot
Match: Q9C5D2 (F-box/LRR-repeat protein 4 OS=Arabidopsis thaliana OX=3702 GN=FBL4 PE=2 SV=1)

HSP 1 Score: 202.2 bits (513), Expect = 1.8e-50
Identity = 167/608 (27.47%), Postives = 294/608 (48.36%), Query Frame = 0

Query: 76  LPDECLFEIFRRLPDRETRSLCACVSKRWLML---------------------------- 135
           LP+E + EIFRRL  +  R  C+ V KRWL L                            
Sbjct: 11  LPEELILEIFRRLESKPNRDACSLVCKRWLSLERFSRTTLRIGASFSPDDFISLLSRRFL 70

Query: 136 -LSSISGNEFYSAS-ENLKP---------TNVVTENEADDQIENDGHLSRNLEGKKATDV 195
            ++SI  +E  S S  +L P         ++  + ++     +     + N+E    TD 
Sbjct: 71  YITSIHVDERISVSLPSLSPSPKRKRGRDSSSPSSSKRKKLTDKTHSGAENVESSSLTDT 130

Query: 196 RLAAIAVGTASCGGLGKLSIRGNNGSQVTNIGLKAVAHGCPSLKAISLWNLSSIGDEGLI 255
            L A+A G      L  +         V+++GL ++A  C SLK++ L     +GD+GL 
Sbjct: 131 GLTALANGFPRIENLSLIWC-----PNVSSVGLCSLAQKCTSLKSLDLQG-CYVGDQGLA 190

Query: 256 EIAKGCQLLEKLDLCQCPGISNKALLELAKHCS-NLTDITIESCANIGSESLQAIGQYCS 315
            + K C+ LE+L+L  C G+++  +++L   CS +L  I + + A I   SL+A+G +C 
Sbjct: 191 AVGKFCKQLEELNLRFCEGLTDVGVIDLVVGCSKSLKSIGVAASAKITDLSLEAVGSHCK 250

Query: 316 NLKSISIRDCPLIGDQGIASLFSSTSYTLNKVKLQGLDVTDVSLAVIGHYGRAVTDLMLT 375
            L+ + + D   I D+G+ ++ +   + L  +KLQ + VTDV+ A +G    ++  L L 
Sbjct: 251 LLEVLYL-DSEYIHDKGLIAV-AQGCHRLKNLKLQCVSVTDVAFAAVGELCTSLERLALY 310

Query: 376 GLTNVTERGFWVMGNGHGLQKLKSLTLTSCHGVTDVGLQSIGKGCPNLRKFCLRKCSFLS 435
              + T++G   +G G   +KLK LTL+ C+ V+  GL++I  GC  L            
Sbjct: 311 SFQHFTDKGMRAIGKGS--KKLKDLTLSDCYFVSCKGLEAIAHGCKEL------------ 370

Query: 436 DNGMVSFAQAAASIENLQLEECHRITQLGLFGTILNCGAKLKALSLVNCLGIKDLSLNLP 495
                         E +++  CH I   G+     +C  +LK L+L+ C  I + +L   
Sbjct: 371 --------------ERVEINGCHNIGTRGIEAIGKSC-PRLKELALLYCQRIGNSALQEI 430

Query: 496 SLTPCKSLQSLSICNCPGFGDASLTLLSKLCPQLQHVNLSGLNGITDSGLLPLLKNCEAG 555
               CKSL+ L + +C G GD ++  ++K C  L+ +++     I + G++ + K+C++ 
Sbjct: 431 G-KGCKSLEILHLVDCSGIGDIAMCSIAKGCRNLKKLHIRRCYEIGNKGIISIGKHCKS- 490

Query: 556 LVKVNLSGCVNLTDKVISSLTKLHGWTLEVLNLDGCSKVTDLSLVAIAENCPLLNDLDIS 615
           L +++L  C  + +K + ++ K  G +L+ LN+ GC++++D  + AIA  CP L  LDIS
Sbjct: 491 LTELSLRFCDKVGNKALIAIGK--GCSLQQLNVSGCNQISDAGITAIARGCPQLTHLDIS 550

Query: 616 KC-CISDFGIAALAQANQFNLQILSVFGCSALTDKSLFALVKLGDSLLGLNLQHCNSIST 643
               I D  +A L +     L+ L +  C  +TD  L  LV+    L   ++ +C  I++
Sbjct: 551 VLQNIGDMPLAELGEGCPM-LKDLVLSHCHHITDNGLNHLVQKCKLLETCHMVYCPGITS 576

BLAST of Lag0004605 vs. ExPASy Swiss-Prot
Match: Q9CZV8 (F-box/LRR-repeat protein 20 OS=Mus musculus OX=10090 GN=Fbxl20 PE=1 SV=3)

HSP 1 Score: 138.7 bits (348), Expect = 2.4e-31
Identity = 119/433 (27.48%), Postives = 190/433 (43.88%), Query Frame = 0

Query: 76  LPDECLFEIFRRLPDRETRSLCACVSKRWLMLLSSISGNEFYSASENLKPTNVVTENEAD 135
           LP E L  IF  L D  T   CA VS+ W                      NV+  + ++
Sbjct: 28  LPKELLLRIFSFL-DVVTLCRCAQVSRAW----------------------NVLALDGSN 87

Query: 136 DQIENDGHLSRNLEGKKATDVRLAAIAVGTASCGG-LGKLSIRGNNGSQVTNIGLKAVAH 195
            Q  +     R++EG+   ++        +  CGG L KLS+RG  G  V +  L+  A 
Sbjct: 88  WQRIDLFDFQRDIEGRVVENI--------SKRCGGFLRKLSLRGCLG--VGDNALRTFAQ 147

Query: 196 GCPSLKAISLWNLSSIGDEGLIEIAKGCQLLEKLDLCQCPGISNKALLELAKHCSNLTDI 255
            C +++ +SL   +   D     ++K C  L  LDL  C  I+N +L  L++ C  L  +
Sbjct: 148 NCRNIEVLSLNGCTKTTDATCTSLSKFCSKLRHLDLASCTSITNMSLKALSEGCPLLEQL 207

Query: 256 TIESCANIGSESLQAIGQYCSNLKSISIRDCPLIGDQGIASLFSSTSYTLNKVKLQGLDV 315
            I  C  +  + +QA+ + C  LK++ ++ C  + D+                       
Sbjct: 208 NISWCDQVTKDGIQALVRGCGGLKALFLKGCTQLEDE----------------------- 267

Query: 316 TDVSLAVIGHYGRAVTDLMLTGLTNVTERGFWVMGNGHGLQKLKSLTLTSCHGVTDVGLQ 375
              +L  IG +   +  L L     +T+ G   +    G  KL+SL  + C  +TD  L 
Sbjct: 268 ---ALKYIGAHCPELVTLNLQTCLQITDEGLITI--CRGCHKLQSLCASGCSNITDAILN 327

Query: 376 SIGKGCPNLRKFCLRKCSFLSDNGMVSFAQAAASIENLQLEECHRITQLGLFGTILNCGA 435
           ++G+ CP LR   + +CS L+D G  + A+    +E + LEEC +IT   L    ++C  
Sbjct: 328 ALGQNCPRLRILEVARCSQLTDVGFTTLARNCHELEKMDLEECVQITDSTLIQLSIHC-P 387

Query: 436 KLKALSLVNCLGIKDLSLNLPSLTPC--KSLQSLSICNCPGFGDASLTLLSKLCPQLQHV 495
           +L+ LSL +C  I D  +       C    L+ + + NCP   DASL  L K C  L+ +
Sbjct: 388 RLQVLSLSHCELITDDGIRHLGNGACAHDQLEVIELDNCPLITDASLEHL-KSCHSLERI 397

Query: 496 NLSGLNGITDSGL 506
            L     IT +G+
Sbjct: 448 ELYDCQQITRAGI 397

BLAST of Lag0004605 vs. ExPASy Swiss-Prot
Match: Q58DG6 (F-box/LRR-repeat protein 20 OS=Bos taurus OX=9913 GN=FBXL20 PE=2 SV=2)

HSP 1 Score: 137.5 bits (345), Expect = 5.4e-31
Identity = 118/433 (27.25%), Postives = 190/433 (43.88%), Query Frame = 0

Query: 76  LPDECLFEIFRRLPDRETRSLCACVSKRWLMLLSSISGNEFYSASENLKPTNVVTENEAD 135
           LP E L  IF  L D  T   CA VS+ W                      NV+  + ++
Sbjct: 28  LPKELLLRIFSFL-DVVTLCRCAQVSRAW----------------------NVLALDGSN 87

Query: 136 DQIENDGHLSRNLEGKKATDVRLAAIAVGTASCGG-LGKLSIRGNNGSQVTNIGLKAVAH 195
            Q  +     R++EG+   ++        +  CGG L KLS+RG  G  V +  L+  A 
Sbjct: 88  WQRIDLFDFQRDIEGRVVENI--------SKRCGGFLRKLSLRGCLG--VGDNALRTFAQ 147

Query: 196 GCPSLKAISLWNLSSIGDEGLIEIAKGCQLLEKLDLCQCPGISNKALLELAKHCSNLTDI 255
            C +++ ++L   +   D     ++K C  L  LDL  C  I+N +L  L++ C  L  +
Sbjct: 148 NCRNIEVLNLNGCTKTTDATCTSLSKFCSKLRHLDLASCTSITNMSLKALSEGCPLLEQL 207

Query: 256 TIESCANIGSESLQAIGQYCSNLKSISIRDCPLIGDQGIASLFSSTSYTLNKVKLQGLDV 315
            I  C  +  + +QA+ + C  LK++ ++ C  + D+                       
Sbjct: 208 NISWCDQVTKDGIQALVRGCGGLKALFLKGCTQLEDE----------------------- 267

Query: 316 TDVSLAVIGHYGRAVTDLMLTGLTNVTERGFWVMGNGHGLQKLKSLTLTSCHGVTDVGLQ 375
              +L  IG +   +  L L     +T+ G   +    G  KL+SL  + C  +TD  L 
Sbjct: 268 ---ALKYIGAHCPELVTLNLQTCLQITDEGLITI--CRGCHKLQSLCASGCSNITDAILN 327

Query: 376 SIGKGCPNLRKFCLRKCSFLSDNGMVSFAQAAASIENLQLEECHRITQLGLFGTILNCGA 435
           ++G+ CP LR   + +CS L+D G  + A+    +E + LEEC +IT   L    ++C  
Sbjct: 328 ALGQNCPRLRILEVARCSQLTDVGFTTLARNCHELEKMDLEECVQITDSTLIQLSIHC-P 387

Query: 436 KLKALSLVNCLGIKDLSLNLPSLTPC--KSLQSLSICNCPGFGDASLTLLSKLCPQLQHV 495
           +L+ LSL +C  I D  +       C    L+ + + NCP   DASL  L K C  L+ +
Sbjct: 388 RLQVLSLSHCELITDDGIRHLGNGACAHDQLEVIELDNCPLITDASLEHL-KSCHSLERI 397

Query: 496 NLSGLNGITDSGL 506
            L     IT +G+
Sbjct: 448 ELYDCQQITRAGI 397

BLAST of Lag0004605 vs. ExPASy TrEMBL
Match: A0A5A7VCC2 (EIN3-binding F-box protein 1 OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold82G002420 PE=4 SV=1)

HSP 1 Score: 1140.6 bits (2949), Expect = 0.0e+00
Identity = 582/653 (89.13%), Postives = 614/653 (94.03%), Query Frame = 0

Query: 1   MYQLISFSSLVLAVTESEDFFPGAPIYQNPKDTSLFLSLGRHVDV-YTLRKRSRVNAPFV 60
           MYQLI + SL LAV ESEDFFP APIY NPKDTSLFLSLG HVDV Y LRKRSRV+AP+V
Sbjct: 23  MYQLI-YCSLDLAVKESEDFFP-APIYPNPKDTSLFLSLGPHVDVYYPLRKRSRVSAPYV 82

Query: 61  PSGEILKKRKTTSIEVLPDECLFEIFRRLPDRETRSLCACVSKRWLMLLSSISGNEFYSA 120
           PSGEILKK K  SIEVLPDECLFEIF+RLPDRETRSLCACVSKRWLMLLSSISGNEFYSA
Sbjct: 83  PSGEILKK-KEASIEVLPDECLFEIFKRLPDRETRSLCACVSKRWLMLLSSISGNEFYSA 142

Query: 121 SENLKPTNVVTENEADDQIENDGHLSRNLEGKKATDVRLAAIAVGTASCGGLGKLSIR-G 180
           SENLKP  VVTEN  D+QI N+G LSRNLEGKKATDVRLAAIAVGTASCGGLGKLSIR G
Sbjct: 143 SENLKPKIVVTENLEDNQITNEGCLSRNLEGKKATDVRLAAIAVGTASCGGLGKLSIRGG 202

Query: 181 NNGSQVTNIGLKAVAHGCPSLKAISLWNLSSIGDEGLIEIAKGCQLLEKLDLCQCPGISN 240
           N+G++VTN+GLKAVAHGCP+LKAISLWNLSSIGDEG+IEIAKGCQLLEKLDL QCPGISN
Sbjct: 203 NHGTEVTNLGLKAVAHGCPALKAISLWNLSSIGDEGMIEIAKGCQLLEKLDLSQCPGISN 262

Query: 241 KALLELAKHCSNLTDITIESCANIGSESLQAIGQYCSNLKSISIRDCPLIGDQGIASLFS 300
           KALLELAKHC NLTDITIE+CANIG+ES+QAIGQYCSNLKSISIRDCPLIGDQGI+SLFS
Sbjct: 263 KALLELAKHCPNLTDITIEACANIGNESIQAIGQYCSNLKSISIRDCPLIGDQGISSLFS 322

Query: 301 STSYTLNKVKLQGLDVTDVSLAVIGHYGRAVTDLMLTGLTNVTERGFWVMGNGHGLQKLK 360
           STSYTLNK KLQGL+VTDVSLAVIGHYGRA+TDL LTGLTNV+ERGFW MGNGHGLQKL+
Sbjct: 323 STSYTLNKAKLQGLNVTDVSLAVIGHYGRAITDLTLTGLTNVSERGFWAMGNGHGLQKLR 382

Query: 361 SLTLTSCHGVTDVGLQSIGKGCPNLRKFCLRKCSFLSDNGMVSFAQAAASIENLQLEECH 420
           S TL+SCHGVTDVGLQSIGKGCPN++KFCLRKCSFLSDNGMVSF QAA SIENLQLEECH
Sbjct: 383 SFTLSSCHGVTDVGLQSIGKGCPNMKKFCLRKCSFLSDNGMVSFVQAATSIENLQLEECH 442

Query: 421 RITQLGLFGTILNCGAKLKALSLVNCLGIKDLSLNLPSLTPCKSLQSLSICNCPGFGDAS 480
           RITQLGLFGTILNCGAKLKALSLVNCLGIKDLSLNLPSL+ CKSLQSLSI NCPGFG+AS
Sbjct: 443 RITQLGLFGTILNCGAKLKALSLVNCLGIKDLSLNLPSLSSCKSLQSLSIRNCPGFGNAS 502

Query: 481 LTLLSKLCPQLQHVNLSGLNGITDSGLLPLLKNCEAGLVKVNLSGCVNLTDKVISSLTKL 540
           LTLLSKLCPQLQHV  SGLNGI DSGLLPL  NC+AGLV VNLSGCVNLTDKVISSLTKL
Sbjct: 503 LTLLSKLCPQLQHVEFSGLNGIRDSGLLPLFMNCKAGLVNVNLSGCVNLTDKVISSLTKL 562

Query: 541 HGWTLEVLNLDGCSKVTDLSLVAIAENCPLLNDLDISKCCISDFGIAALAQANQFNLQIL 600
           HGWTLE+LNLDGC KVTD SLVAIAENCPLLNDLD+SKCCI+DFG+AALAQANQFNLQ+L
Sbjct: 563 HGWTLELLNLDGCLKVTDSSLVAIAENCPLLNDLDVSKCCITDFGVAALAQANQFNLQLL 622

Query: 601 SVFGCSALTDKSLFALVKLGDSLLGLNLQHCNSISTRSIELLLAQLRRCDILY 652
           SVFGCSALTD+SL ALVKLGDSLLGLNLQHCNSISTRSIELLLAQL RCDILY
Sbjct: 623 SVFGCSALTDQSLLALVKLGDSLLGLNLQHCNSISTRSIELLLAQLHRCDILY 672

BLAST of Lag0004605 vs. ExPASy TrEMBL
Match: A0A6J1KNG4 (EIN3-binding F-box protein 1-like OS=Cucurbita maxima OX=3661 GN=LOC111496162 PE=4 SV=1)

HSP 1 Score: 1135.2 bits (2935), Expect = 0.0e+00
Identity = 572/640 (89.38%), Postives = 605/640 (94.53%), Query Frame = 0

Query: 14  VTESEDFFPGAPIYQNPKDTSLFLSLGRHVDV-YTLRKRSRVNAPFVPSGEILKKRKTTS 73
           +TESEDFF GAPIYQNPKDTSLFLSLGR VDV Y LRKRSR    FVPSGEILKK+K TS
Sbjct: 9   LTESEDFFSGAPIYQNPKDTSLFLSLGRRVDVYYPLRKRSRATESFVPSGEILKKKK-TS 68

Query: 74  IEVLPDECLFEIFRRLPDRETRSLCACVSKRWLMLLSSISGNEFYSASENLKPTNVVTEN 133
           IE+LPDECLFE+FRRLPDRETRSLCACVSKRWLMLLSSISGNEFYSASENL+P NVV  N
Sbjct: 69  IEILPDECLFEVFRRLPDRETRSLCACVSKRWLMLLSSISGNEFYSASENLRPENVVAVN 128

Query: 134 EADDQIENDGHLSRNLEGKKATDVRLAAIAVGTASCGGLGKLSIRGN-NGSQVTNIGLKA 193
           E D+++ENDGHLSRNLEG+KATD+RLAAIAVGTASCGGLGKLSIRG  +GS+V+NIGLKA
Sbjct: 129 ETDNKVENDGHLSRNLEGEKATDIRLAAIAVGTASCGGLGKLSIRGGIHGSKVSNIGLKA 188

Query: 194 VAHGCPSLKAISLWNLSSIGDEGLIEIAKGCQLLEKLDLCQCPGISNKALLELAKHCSNL 253
           VA GCPSLKAISLWNLSSIGDEGLIEIAKGCQ LEKLDLCQCPGISNKALLELAK+C NL
Sbjct: 189 VARGCPSLKAISLWNLSSIGDEGLIEIAKGCQFLEKLDLCQCPGISNKALLELAKNCPNL 248

Query: 254 TDITIESCANIGSESLQAIGQYCSNLKSISIRDCPLIGDQGIASLFSSTSYTLNKVKLQG 313
           TDITIESCANIG+E LQA+GQYCSNLKSISIRDCPLIGDQG+ASLF ST+ TL+KVKLQG
Sbjct: 249 TDITIESCANIGNEGLQALGQYCSNLKSISIRDCPLIGDQGLASLFFSTADTLSKVKLQG 308

Query: 314 LDVTDVSLAVIGHYGRAVTDLMLTGLTNVTERGFWVMGNGHGLQKLKSLTLTSCHGVTDV 373
           L+VTD+SLAVIGHYGR VTDLMLTGLTNVTE+GFWVMG+GHGLQKLKS TL SCHGVTDV
Sbjct: 309 LNVTDMSLAVIGHYGREVTDLMLTGLTNVTEKGFWVMGSGHGLQKLKSFTLASCHGVTDV 368

Query: 374 GLQSIGKGCPNLRKFCLRKCSFLSDNGMVSFAQAAASIENLQLEECHRITQLGLFGTILN 433
           GLQSI KGCPNL+KFCLRKCSFLSD GMVSF QAA SIENLQLEECHRITQLGLFGTILN
Sbjct: 369 GLQSIAKGCPNLKKFCLRKCSFLSDKGMVSFTQAALSIENLQLEECHRITQLGLFGTILN 428

Query: 434 CGAKLKALSLVNCLGIKDLSLNLPSLTPCKSLQSLSICNCPGFGDASLTLLSKLCPQLQH 493
           CGAKLKALSLVNCLGIKDLSLNLPS TPCKSLQSLSICNCPGFG+ SLTLLSKLCPQLQH
Sbjct: 429 CGAKLKALSLVNCLGIKDLSLNLPSTTPCKSLQSLSICNCPGFGNVSLTLLSKLCPQLQH 488

Query: 494 VNLSGLNGITDSGLLPLLKNCEAGLVKVNLSGCVNLTDKVISSLTKLHGWTLEVLNLDGC 553
           VNLSGL+GI+DSGLLPLLKNCEAGLV VNLSGCVNLTDKVISSLTKLHGWTLEVLN+DGC
Sbjct: 489 VNLSGLSGISDSGLLPLLKNCEAGLVNVNLSGCVNLTDKVISSLTKLHGWTLEVLNVDGC 548

Query: 554 SKVTDLSLVAIAENCPLLNDLDISKCCISDFGIAALAQANQFNLQILSVFGCSALTDKSL 613
           SKVTD SLVAIAENCPLLNDLD+SKC I+DFG+AALAQANQFNLQILSVFGCSALTDKSL
Sbjct: 549 SKVTDSSLVAIAENCPLLNDLDVSKCRITDFGVAALAQANQFNLQILSVFGCSALTDKSL 608

Query: 614 FALVKLGDSLLGLNLQHCNSISTRSIELLLAQLRRCDILY 652
           FAL KLGDSLLGLNL++CNSISTRSIELLLAQL RCDILY
Sbjct: 609 FALFKLGDSLLGLNLRNCNSISTRSIELLLAQLHRCDILY 647

BLAST of Lag0004605 vs. ExPASy TrEMBL
Match: A0A0A0LLZ6 (F-box domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_2G381760 PE=4 SV=1)

HSP 1 Score: 1133.6 bits (2931), Expect = 0.0e+00
Identity = 572/640 (89.38%), Postives = 603/640 (94.22%), Query Frame = 0

Query: 14  VTESEDFFPGAPIYQNPKDTSLFLSLGRHVDV-YTLRKRSRVNAPFVPSGEILKKRKTTS 73
           +TESEDFFPGAPIY NPKDTSLFLSLGR VDV Y LRKRSRV+APFVPSGEILKK    S
Sbjct: 9   LTESEDFFPGAPIYPNPKDTSLFLSLGRQVDVYYPLRKRSRVSAPFVPSGEILKKE--AS 68

Query: 74  IEVLPDECLFEIFRRLPDRETRSLCACVSKRWLMLLSSISGNEFYSASENLKPTNVVTEN 133
           IEVLPDECLFEIF+RLP+RETRSLCACVSKRWLMLLSSISGNEFY ASENLKP NVVTEN
Sbjct: 69  IEVLPDECLFEIFKRLPERETRSLCACVSKRWLMLLSSISGNEFYGASENLKPKNVVTEN 128

Query: 134 EADDQIENDGHLSRNLEGKKATDVRLAAIAVGTASCGGLGKLSIR-GNNGSQVTNIGLKA 193
             D+Q  N+G LSRNLEGKKATDVRLAAIAVGTASCGGLGKLSIR GN+GS+VTN+GLKA
Sbjct: 129 LEDNQTTNEGCLSRNLEGKKATDVRLAAIAVGTASCGGLGKLSIRGGNHGSEVTNLGLKA 188

Query: 194 VAHGCPSLKAISLWNLSSIGDEGLIEIAKGCQLLEKLDLCQCPGISNKALLELAKHCSNL 253
           VAHGCP LKAISLWNLSSIGDEGLIEIAKGCQLLEKLDL QCPGISNKALLELAK+C NL
Sbjct: 189 VAHGCPGLKAISLWNLSSIGDEGLIEIAKGCQLLEKLDLSQCPGISNKALLELAKNCPNL 248

Query: 254 TDITIESCANIGSESLQAIGQYCSNLKSISIRDCPLIGDQGIASLFSSTSYTLNKVKLQG 313
           TDIT+E+CANIG+ES+QAIGQYCSNLKSISIRDCPLIGDQGI+SLFSSTSYTLNK KLQG
Sbjct: 249 TDITVEACANIGNESVQAIGQYCSNLKSISIRDCPLIGDQGISSLFSSTSYTLNKAKLQG 308

Query: 314 LDVTDVSLAVIGHYGRAVTDLMLTGLTNVTERGFWVMGNGHGLQKLKSLTLTSCHGVTDV 373
           L+VTDVSLAVIGHYGRA+TDL LTGLTNV+ERGFW MGNGHGLQKL+S TL+SCHGVTDV
Sbjct: 309 LNVTDVSLAVIGHYGRAITDLTLTGLTNVSERGFWAMGNGHGLQKLRSFTLSSCHGVTDV 368

Query: 374 GLQSIGKGCPNLRKFCLRKCSFLSDNGMVSFAQAAASIENLQLEECHRITQLGLFGTILN 433
           GLQSIGKGCPNL+KFCL KCSFLSDNGMVSF QAA SIENLQLEECHRITQLGLFGTILN
Sbjct: 369 GLQSIGKGCPNLKKFCLHKCSFLSDNGMVSFVQAATSIENLQLEECHRITQLGLFGTILN 428

Query: 434 CGAKLKALSLVNCLGIKDLSLNLPSLTPCKSLQSLSICNCPGFGDASLTLLSKLCPQLQH 493
           CGAKLKALSLVNCLGIKDLSLNLPSL+ CKSLQSLSI NCPGFG+ASLTLLSKLCPQLQH
Sbjct: 429 CGAKLKALSLVNCLGIKDLSLNLPSLSSCKSLQSLSIRNCPGFGNASLTLLSKLCPQLQH 488

Query: 494 VNLSGLNGITDSGLLPLLKNCEAGLVKVNLSGCVNLTDKVISSLTKLHGWTLEVLNLDGC 553
           V  SGLN ITDSGLLPL  NC+AGLVKVNLSGCVNLTDKVISSLTKLHGWTLE+LNLDGC
Sbjct: 489 VEFSGLNAITDSGLLPLFMNCKAGLVKVNLSGCVNLTDKVISSLTKLHGWTLELLNLDGC 548

Query: 554 SKVTDLSLVAIAENCPLLNDLDISKCCISDFGIAALAQANQFNLQILSVFGCSALTDKSL 613
            KVTD SLVAIAENCPLLNDLD+SKCCI+DFG+AALAQANQFNLQ+LSV+GCSALTD+SL
Sbjct: 549 LKVTDSSLVAIAENCPLLNDLDVSKCCITDFGVAALAQANQFNLQLLSVYGCSALTDQSL 608

Query: 614 FALVKLGDSLLGLNLQHCNSISTRSIELLLAQLRRCDILY 652
            ALVKLGDSLLGLNLQHCNSISTRSIELLLAQL RCDILY
Sbjct: 609 LALVKLGDSLLGLNLQHCNSISTRSIELLLAQLHRCDILY 646

BLAST of Lag0004605 vs. ExPASy TrEMBL
Match: A0A6J1GHL0 (EIN3-binding F-box protein 1-like OS=Cucurbita moschata OX=3662 GN=LOC111454234 PE=4 SV=1)

HSP 1 Score: 1129.8 bits (2921), Expect = 0.0e+00
Identity = 569/640 (88.91%), Postives = 605/640 (94.53%), Query Frame = 0

Query: 14  VTESEDFFPGAPIYQNPKDTSLFLSLGRHVDV-YTLRKRSRVNAPFVPSGEILKKRKTTS 73
           +TESEDFF GAPIYQNPKDTSLFLSLGR VDV Y LRKRSR    FV SGEILKK+K TS
Sbjct: 9   LTESEDFFSGAPIYQNPKDTSLFLSLGRRVDVYYPLRKRSRATESFVASGEILKKKK-TS 68

Query: 74  IEVLPDECLFEIFRRLPDRETRSLCACVSKRWLMLLSSISGNEFYSASENLKPTNVVTEN 133
           IE+LPDECLFE+FRRLPDRETRSLCACVSKRWLMLLSSISGNEFYSASENL+P NVV  N
Sbjct: 69  IEILPDECLFEVFRRLPDRETRSLCACVSKRWLMLLSSISGNEFYSASENLRPENVVAVN 128

Query: 134 EADDQIENDGHLSRNLEGKKATDVRLAAIAVGTASCGGLGKLSIRGN-NGSQVTNIGLKA 193
           E D+++ENDGHLSRNLEG+KA+D+RLAAIAVGTASCGGLGKLSIRG  +GS+V+NIGLKA
Sbjct: 129 ETDNKVENDGHLSRNLEGEKASDIRLAAIAVGTASCGGLGKLSIRGGIHGSKVSNIGLKA 188

Query: 194 VAHGCPSLKAISLWNLSSIGDEGLIEIAKGCQLLEKLDLCQCPGISNKALLELAKHCSNL 253
           VA GCPSLKAISLWNLSSIGDEGLIEIAKGCQ LEKLDLCQCPGISNKALLELAK+C NL
Sbjct: 189 VARGCPSLKAISLWNLSSIGDEGLIEIAKGCQFLEKLDLCQCPGISNKALLELAKNCPNL 248

Query: 254 TDITIESCANIGSESLQAIGQYCSNLKSISIRDCPLIGDQGIASLFSSTSYTLNKVKLQG 313
           TDITIESCANIG+E LQA+GQYCSNLKSISIRDCPLIGDQG+ASLF ST+ TL+KVKLQG
Sbjct: 249 TDITIESCANIGNEGLQALGQYCSNLKSISIRDCPLIGDQGLASLFFSTADTLSKVKLQG 308

Query: 314 LDVTDVSLAVIGHYGRAVTDLMLTGLTNVTERGFWVMGNGHGLQKLKSLTLTSCHGVTDV 373
           L+VTD+SLAVIGHYGRAVTDLMLTGLTNVTE+GFWVMGNGHGLQKLKS TL+SCHGVTDV
Sbjct: 309 LNVTDMSLAVIGHYGRAVTDLMLTGLTNVTEKGFWVMGNGHGLQKLKSFTLSSCHGVTDV 368

Query: 374 GLQSIGKGCPNLRKFCLRKCSFLSDNGMVSFAQAAASIENLQLEECHRITQLGLFGTILN 433
           GLQSI KGCPNL+KFCLRKCSFLSD GMVSF QAA SIENLQLEECHRITQLGLFGTILN
Sbjct: 369 GLQSIAKGCPNLKKFCLRKCSFLSDKGMVSFTQAALSIENLQLEECHRITQLGLFGTILN 428

Query: 434 CGAKLKALSLVNCLGIKDLSLNLPSLTPCKSLQSLSICNCPGFGDASLTLLSKLCPQLQH 493
           CGAKLKALSLVNCLGIKDLSLNLPS TPCKSLQSLSICNCPGFG+ SLTLLSKLCPQLQH
Sbjct: 429 CGAKLKALSLVNCLGIKDLSLNLPSTTPCKSLQSLSICNCPGFGNVSLTLLSKLCPQLQH 488

Query: 494 VNLSGLNGITDSGLLPLLKNCEAGLVKVNLSGCVNLTDKVISSLTKLHGWTLEVLNLDGC 553
           VNLSGL+GI+DSGLLPLLKNCEAGLV VNLSGCVNLTDKVISSLTKLHGWTLEVLN+DGC
Sbjct: 489 VNLSGLSGISDSGLLPLLKNCEAGLVNVNLSGCVNLTDKVISSLTKLHGWTLEVLNVDGC 548

Query: 554 SKVTDLSLVAIAENCPLLNDLDISKCCISDFGIAALAQANQFNLQILSVFGCSALTDKSL 613
           SKVTD SLVAI+ENCPLLNDLD+SKC I+DFG+AALAQANQFNLQILSVFGCSALTDKSL
Sbjct: 549 SKVTDSSLVAISENCPLLNDLDVSKCGITDFGVAALAQANQFNLQILSVFGCSALTDKSL 608

Query: 614 FALVKLGDSLLGLNLQHCNSISTRSIELLLAQLRRCDILY 652
           F L+KLGDSLLGLNL++CN ISTRSIELLLAQL RCDILY
Sbjct: 609 FTLMKLGDSLLGLNLRNCNLISTRSIELLLAQLLRCDILY 647

BLAST of Lag0004605 vs. ExPASy TrEMBL
Match: A0A1S3BBN9 (EIN3-binding F-box protein 1 OS=Cucumis melo OX=3656 GN=LOC103488312 PE=4 SV=1)

HSP 1 Score: 1129.4 bits (2920), Expect = 0.0e+00
Identity = 572/638 (89.66%), Postives = 603/638 (94.51%), Query Frame = 0

Query: 16  ESEDFFPGAPIYQNPKDTSLFLSLGRHVDV-YTLRKRSRVNAPFVPSGEILKKRKTTSIE 75
           ESEDFFP APIY NPKDTSLFLSLG HVDV Y LRKRSRV+AP+VPSGEILKK K  SIE
Sbjct: 11  ESEDFFP-APIYPNPKDTSLFLSLGPHVDVYYPLRKRSRVSAPYVPSGEILKK-KEASIE 70

Query: 76  VLPDECLFEIFRRLPDRETRSLCACVSKRWLMLLSSISGNEFYSASENLKPTNVVTENEA 135
           VLPDECLFEIF+RLPDRETRSLCACVSKRWLMLLSSISGNEFYSASENLKP  VVTEN  
Sbjct: 71  VLPDECLFEIFKRLPDRETRSLCACVSKRWLMLLSSISGNEFYSASENLKPKIVVTENLE 130

Query: 136 DDQIENDGHLSRNLEGKKATDVRLAAIAVGTASCGGLGKLSIR-GNNGSQVTNIGLKAVA 195
           D+QI N+G LSRNLEGKKATDVRLAAIAVGTASCGGLGKLSIR GN+G++VTN+GLKAVA
Sbjct: 131 DNQITNEGCLSRNLEGKKATDVRLAAIAVGTASCGGLGKLSIRGGNHGTEVTNLGLKAVA 190

Query: 196 HGCPSLKAISLWNLSSIGDEGLIEIAKGCQLLEKLDLCQCPGISNKALLELAKHCSNLTD 255
           HGCP+LKAISLWNLSSIGDEG+IEIAKGCQLLEKLDL QCPGISNKALLELAKHC NLTD
Sbjct: 191 HGCPALKAISLWNLSSIGDEGMIEIAKGCQLLEKLDLSQCPGISNKALLELAKHCPNLTD 250

Query: 256 ITIESCANIGSESLQAIGQYCSNLKSISIRDCPLIGDQGIASLFSSTSYTLNKVKLQGLD 315
           ITIE+CANIG+ES+QAIGQYCSNLKSISIRDCPLIGDQGI+SLFSSTSYTLNK KLQGL+
Sbjct: 251 ITIEACANIGNESIQAIGQYCSNLKSISIRDCPLIGDQGISSLFSSTSYTLNKAKLQGLN 310

Query: 316 VTDVSLAVIGHYGRAVTDLMLTGLTNVTERGFWVMGNGHGLQKLKSLTLTSCHGVTDVGL 375
           VTDVSLAVIGHYGRA+TDL LTGLTNV+ERGFW MGNGHGLQKL+S TL+SCHGVTDVGL
Sbjct: 311 VTDVSLAVIGHYGRAITDLTLTGLTNVSERGFWAMGNGHGLQKLRSFTLSSCHGVTDVGL 370

Query: 376 QSIGKGCPNLRKFCLRKCSFLSDNGMVSFAQAAASIENLQLEECHRITQLGLFGTILNCG 435
           QSIGKGCPN++KFCLRKCSFLSDNGMVSF QAA SIENLQLEECHRITQLGLFGTILNCG
Sbjct: 371 QSIGKGCPNMKKFCLRKCSFLSDNGMVSFVQAATSIENLQLEECHRITQLGLFGTILNCG 430

Query: 436 AKLKALSLVNCLGIKDLSLNLPSLTPCKSLQSLSICNCPGFGDASLTLLSKLCPQLQHVN 495
           AKLKALSLVNCLGIKDLSLNLPSL+ CKSLQSLSI NCPGFG+ASLTLLSKLCPQLQHV 
Sbjct: 431 AKLKALSLVNCLGIKDLSLNLPSLSSCKSLQSLSIRNCPGFGNASLTLLSKLCPQLQHVE 490

Query: 496 LSGLNGITDSGLLPLLKNCEAGLVKVNLSGCVNLTDKVISSLTKLHGWTLEVLNLDGCSK 555
            SGLNGI DSGLLPL  NC+AGLV VNLSGCVNLTDKVISSLTKLHGWTLE+LNLDGC K
Sbjct: 491 FSGLNGIRDSGLLPLFMNCKAGLVNVNLSGCVNLTDKVISSLTKLHGWTLELLNLDGCLK 550

Query: 556 VTDLSLVAIAENCPLLNDLDISKCCISDFGIAALAQANQFNLQILSVFGCSALTDKSLFA 615
           VTD SLVAIAENCPLLNDLD+SKCCI+DFG+AALAQANQFNLQ+LSVFGCSALTD+SL A
Sbjct: 551 VTDSSLVAIAENCPLLNDLDVSKCCITDFGVAALAQANQFNLQLLSVFGCSALTDQSLLA 610

Query: 616 LVKLGDSLLGLNLQHCNSISTRSIELLLAQLRRCDILY 652
           LVKLGDSLLGLNLQHCNSISTRSIELLLAQL RCDILY
Sbjct: 611 LVKLGDSLLGLNLQHCNSISTRSIELLLAQLHRCDILY 646

BLAST of Lag0004605 vs. TAIR 10
Match: AT2G25490.1 (EIN3-binding F box protein 1 )

HSP 1 Score: 740.3 bits (1910), Expect = 1.3e-213
Identity = 379/643 (58.94%), Postives = 492/643 (76.52%), Query Frame = 0

Query: 9   SLVLAVTESEDFFPGAPIYQNPKDTSLFLSLGRHVDVY-TLRKRSRVNAPFVPSGEILKK 68
           S + +     DF+    IY NPKD SL LSLG   DVY    KRSRV AP + S     +
Sbjct: 2   SQIFSFAGENDFYRRGAIYPNPKDASLLLSLGSFADVYFPPSKRSRVVAPTIFSA---FE 61

Query: 69  RKTTSIEVLPDECLFEIFRRLPDRETRSLCACVSKRWLMLLSSISGNEFYSASENLKPTN 128
           +K  SI+VLPDECLFEIFRRL   + RS CA VSK+WL L+SSI   E    S+      
Sbjct: 62  KKPVSIDVLPDECLFEIFRRLSGPQERSACAFVSKQWLTLVSSIRQKEIDVPSK------ 121

Query: 129 VVTENEADDQIENDGHLSRNLEGKKATDVRLAAIAVGTASCGGLGKLSIRGNNGSQVTNI 188
            +TE    D  + +G LSR+L+GKKATDVRLAAIAVGTA  GGLGKLSIRG+N ++V+++
Sbjct: 122 -ITE----DGDDCEGCLSRSLDGKKATDVRLAAIAVGTAGRGGLGKLSIRGSNSAKVSDL 181

Query: 189 GLKAVAHGCPSLKAISLWNLSSIGDEGLIEIAKGCQLLEKLDLCQCPGISNKALLELAKH 248
           GL+++   CPSL ++SLWN+S+I D GL+EIA+GC  LEKL+L +C  I++K L+ +AK 
Sbjct: 182 GLRSIGRSCPSLGSLSLWNVSTITDNGLLEIAEGCAQLEKLELNRCSTITDKGLVAIAKS 241

Query: 249 CSNLTDITIESCANIGSESLQAIGQYCSNLKSISIRDCPLIGDQGIASLFSSTSYTLNKV 308
           C NLT++T+E+C+ IG E L AI + CS LKS+SI++CPL+ DQGIASL S+T+ +L K+
Sbjct: 242 CPNLTELTLEACSRIGDEGLLAIARSCSKLKSVSIKNCPLVRDQGIASLLSNTTCSLAKL 301

Query: 309 KLQGLDVTDVSLAVIGHYGRAVTDLMLTGLTNVTERGFWVMGNGHGLQKLKSLTLTSCHG 368
           KLQ L+VTDVSLAV+GHYG ++TDL+L GL++V+E+GFWVMGNG GLQKL SLT+T+C G
Sbjct: 302 KLQMLNVTDVSLAVVGHYGLSITDLVLAGLSHVSEKGFWVMGNGVGLQKLNSLTITACQG 361

Query: 369 VTDVGLQSIGKGCPNLRKFCLRKCSFLSDNGMVSFAQAAASIENLQLEECHRITQLGLFG 428
           VTD+GL+S+GKGCPN++K  + K   LSDNG+VSFA+A+ S+E+LQLEECHR+TQ G FG
Sbjct: 362 VTDMGLESVGKGCPNMKKAIISKSPLLSDNGLVSFAKASLSLESLQLEECHRVTQFGFFG 421

Query: 429 TILNCGAKLKALSLVNCLGIKDLSLNLPSLTPCKSLQSLSICNCPGFGDASLTLLSKLCP 488
           ++LNCG KLKA SLVNCL I+DL+  LP+ + C +L+SLSI NCPGFGDA+L  + KLCP
Sbjct: 422 SLLNCGEKLKAFSLVNCLSIRDLTTGLPASSHCSALRSLSIRNCPGFGDANLAAIGKLCP 481

Query: 489 QLQHVNLSGLNGITDSGLLPLLKNCEAGLVKVNLSGCVNLTDKVISSLTKLHGWTLEVLN 548
           QL+ ++L GL GIT+SG L L+   ++ LVK+N SGC NLTD+VIS++T  +GWTLEVLN
Sbjct: 482 QLEDIDLCGLKGITESGFLHLI---QSSLVKINFSGCSNLTDRVISAITARNGWTLEVLN 541

Query: 549 LDGCSKVTDLSLVAIAENCPLLNDLDISKCCISDFGIAALAQANQFNLQILSVFGCSALT 608
           +DGCS +TD SLV+IA NC +L+DLDISKC ISD GI ALA +++  LQILSV GCS +T
Sbjct: 542 IDGCSNITDASLVSIAANCQILSDLDISKCAISDSGIQALASSDKLKLQILSVAGCSMVT 601

Query: 609 DKSLFALVKLGDSLLGLNLQHCNSISTRSIELLLAQLRRCDIL 651
           DKSL A+V LG +LLGLNLQ C SIS  +++ L+ +L +CDIL
Sbjct: 602 DKSLPAIVGLGSTLLGLNLQQCRSISNSTVDFLVERLYKCDIL 627

BLAST of Lag0004605 vs. TAIR 10
Match: AT5G25350.1 (EIN3-binding F box protein 2 )

HSP 1 Score: 673.3 bits (1736), Expect = 1.9e-193
Identity = 355/623 (56.98%), Postives = 454/623 (72.87%), Query Frame = 0

Query: 34  SLFLSLGRHVDV-YTLRKRSRVNAPFVPSGEILKKRKTTSIEVLPDECLFEIFRRLPDRE 93
           S++LS G    V Y  RKR RV A    SG    + K TSI+VLP+ECLFEI RRLP  +
Sbjct: 18  SMYLSPGSCPGVYYPARKRLRVAATSFYSG---FEEKQTSIDVLPEECLFEILRRLPSGQ 77

Query: 94  TRSLCACVSKRWLMLLSSISGNEFYSASENLKPTNVVTENEADDQIENDGHLSRNLEGKK 153
            RS CACVSK WL LLSSIS +E             V E+   D  E +G LSR+LEGKK
Sbjct: 78  ERSACACVSKHWLNLLSSISRSE-------------VNESSVQDVEEGEGFLSRSLEGKK 137

Query: 154 ATDVRLAAIAVGTASCGGLGKLSIRGNN-GSQVTNIGLKAVAHGCPSLKAISLWNLSSIG 213
           ATD+RLAAIAVGT+S GGLGKL IRG+   S+VT++GL AVAHGCPSL+ +SLWNL ++ 
Sbjct: 138 ATDLRLAAIAVGTSSRGGLGKLQIRGSGFESKVTDVGLGAVAHGCPSLRIVSLWNLPAVS 197

Query: 214 DEGLIEIAKGCQLLEKLDLCQCPGISNKALLELAKHCSNLTDITIESCANIGSESLQAIG 273
           D GL EIA+ C ++EKLDL +CPGI++  L+ +A++C NL+D+TI+SC+ +G+E L+AI 
Sbjct: 198 DLGLSEIARSCPMIEKLDLSRCPGITDSGLVAIAENCVNLSDLTIDSCSGVGNEGLRAIA 257

Query: 274 QYCSNLKSISIRDCPLIGDQGIASLFSSTSYTLNKVKLQGLDVTDVSLAVIGHYGRAVTD 333
           + C NL+SISIR CP IGDQG+A L +     L KVKLQ L+V+ +SLAVIGHYG AVTD
Sbjct: 258 RRCVNLRSISIRSCPRIGDQGVAFLLAQAGSYLTKVKLQMLNVSGLSLAVIGHYGAAVTD 317

Query: 334 LMLTGLTNVTERGFWVMGNGHGLQKLKSLTLTSCHGVTDVGLQSIGKGCPNLRKFCLRKC 393
           L+L GL  V E+GFWVMGN  GL+KLKSL++ SC G+TDVGL+++G GCP+L+   L KC
Sbjct: 318 LVLHGLQGVNEKGFWVMGNAKGLKKLKSLSVMSCRGMTDVGLEAVGNGCPDLKHVSLNKC 377

Query: 394 SFLSDNGMVSFAQAAASIENLQLEECHRITQLGLFGTILNCGAKLKALSLVNCLGIKDLS 453
             +S  G+V+ A++A S+E+L+LEECHRI Q GL G ++NCG+KLKA SL NCLGI D +
Sbjct: 378 LLVSGKGLVALAKSALSLESLKLEECHRINQFGLMGFLMNCGSKLKAFSLANCLGISDFN 437

Query: 454 --LNLPSLTPCKSLQSLSICNCPGFGDASLTLLSKLCPQLQHVNLSGLNGITDSGLLPLL 513
              +LPS   C SL+SLSI  CPGFGDASL  L K C QLQ V L GLNG+TD+G+  LL
Sbjct: 438 SESSLPS-PSCSSLRSLSIRCCPGFGDASLAFLGKFCHQLQDVELCGLNGVTDAGVRELL 497

Query: 514 KNCEAGLVKVNLSGCVNLTDKVISSLTKLHGWTLEVLNLDGCSKVTDLSLVAIAENCPLL 573
           ++   GLVKVNLS C+N++D  +S+++  HG TLE LNLDGC  +T+ SLVA+A+NC  +
Sbjct: 498 QSNNVGLVKVNLSECINVSDNTVSAISVCHGRTLESLNLDGCKNITNASLVAVAKNCYSV 557

Query: 574 NDLDISKCCISDFGIAALAQA-NQFNLQILSVFGCSALTDKSLFALVKLGDSLLGLNLQH 633
           NDLDIS   +SD GI ALA + N  NLQ+LS+ GCS++TDKS   + KLG +LLGLN+Q 
Sbjct: 558 NDLDISNTLVSDHGIKALASSPNHLNLQVLSIGGCSSITDKSKACIQKLGRTLLGLNIQR 617

Query: 634 CNSISTRSIELLLAQLRRCDILY 652
           C  IS+ +++ LL  L RCDILY
Sbjct: 618 CGRISSSTVDTLLENLWRCDILY 623

BLAST of Lag0004605 vs. TAIR 10
Match: AT4G15475.1 (F-box/RNI-like superfamily protein )

HSP 1 Score: 202.2 bits (513), Expect = 1.3e-51
Identity = 167/608 (27.47%), Postives = 294/608 (48.36%), Query Frame = 0

Query: 76  LPDECLFEIFRRLPDRETRSLCACVSKRWLML---------------------------- 135
           LP+E + EIFRRL  +  R  C+ V KRWL L                            
Sbjct: 11  LPEELILEIFRRLESKPNRDACSLVCKRWLSLERFSRTTLRIGASFSPDDFISLLSRRFL 70

Query: 136 -LSSISGNEFYSAS-ENLKP---------TNVVTENEADDQIENDGHLSRNLEGKKATDV 195
            ++SI  +E  S S  +L P         ++  + ++     +     + N+E    TD 
Sbjct: 71  YITSIHVDERISVSLPSLSPSPKRKRGRDSSSPSSSKRKKLTDKTHSGAENVESSSLTDT 130

Query: 196 RLAAIAVGTASCGGLGKLSIRGNNGSQVTNIGLKAVAHGCPSLKAISLWNLSSIGDEGLI 255
            L A+A G      L  +         V+++GL ++A  C SLK++ L     +GD+GL 
Sbjct: 131 GLTALANGFPRIENLSLIWC-----PNVSSVGLCSLAQKCTSLKSLDLQG-CYVGDQGLA 190

Query: 256 EIAKGCQLLEKLDLCQCPGISNKALLELAKHCS-NLTDITIESCANIGSESLQAIGQYCS 315
            + K C+ LE+L+L  C G+++  +++L   CS +L  I + + A I   SL+A+G +C 
Sbjct: 191 AVGKFCKQLEELNLRFCEGLTDVGVIDLVVGCSKSLKSIGVAASAKITDLSLEAVGSHCK 250

Query: 316 NLKSISIRDCPLIGDQGIASLFSSTSYTLNKVKLQGLDVTDVSLAVIGHYGRAVTDLMLT 375
            L+ + + D   I D+G+ ++ +   + L  +KLQ + VTDV+ A +G    ++  L L 
Sbjct: 251 LLEVLYL-DSEYIHDKGLIAV-AQGCHRLKNLKLQCVSVTDVAFAAVGELCTSLERLALY 310

Query: 376 GLTNVTERGFWVMGNGHGLQKLKSLTLTSCHGVTDVGLQSIGKGCPNLRKFCLRKCSFLS 435
              + T++G   +G G   +KLK LTL+ C+ V+  GL++I  GC  L            
Sbjct: 311 SFQHFTDKGMRAIGKGS--KKLKDLTLSDCYFVSCKGLEAIAHGCKEL------------ 370

Query: 436 DNGMVSFAQAAASIENLQLEECHRITQLGLFGTILNCGAKLKALSLVNCLGIKDLSLNLP 495
                         E +++  CH I   G+     +C  +LK L+L+ C  I + +L   
Sbjct: 371 --------------ERVEINGCHNIGTRGIEAIGKSC-PRLKELALLYCQRIGNSALQEI 430

Query: 496 SLTPCKSLQSLSICNCPGFGDASLTLLSKLCPQLQHVNLSGLNGITDSGLLPLLKNCEAG 555
               CKSL+ L + +C G GD ++  ++K C  L+ +++     I + G++ + K+C++ 
Sbjct: 431 G-KGCKSLEILHLVDCSGIGDIAMCSIAKGCRNLKKLHIRRCYEIGNKGIISIGKHCKS- 490

Query: 556 LVKVNLSGCVNLTDKVISSLTKLHGWTLEVLNLDGCSKVTDLSLVAIAENCPLLNDLDIS 615
           L +++L  C  + +K + ++ K  G +L+ LN+ GC++++D  + AIA  CP L  LDIS
Sbjct: 491 LTELSLRFCDKVGNKALIAIGK--GCSLQQLNVSGCNQISDAGITAIARGCPQLTHLDIS 550

Query: 616 KC-CISDFGIAALAQANQFNLQILSVFGCSALTDKSLFALVKLGDSLLGLNLQHCNSIST 643
               I D  +A L +     L+ L +  C  +TD  L  LV+    L   ++ +C  I++
Sbjct: 551 VLQNIGDMPLAELGEGCPM-LKDLVLSHCHHITDNGLNHLVQKCKLLETCHMVYCPGITS 576

BLAST of Lag0004605 vs. TAIR 10
Match: AT5G27920.1 (F-box family protein )

HSP 1 Score: 157.1 bits (396), Expect = 4.7e-38
Identity = 141/525 (26.86%), Postives = 232/525 (44.19%), Query Frame = 0

Query: 169 GGLGKLSIRGNNGSQVTNI---GLKAVAHGCPSLKAISLWNLSSIGDEGLIEIAKGCQLL 228
           G +  L I+  N S+ T +   GL+ +A  C +L+ + + +    GD     ++    L 
Sbjct: 90  GAISTLGIKSLNLSRSTAVRARGLETLARMCHALERVDVSHCWGFGDREAAALSSATGLR 149

Query: 229 EKLDLCQCPGISNKALLELAKHCSNLTDITIESCANIGSESLQAIGQYCSNLKSI----- 288
           E L + +C  +S+  L  +   CSNL  I+++ C  I    +  + + C  LKS+     
Sbjct: 150 E-LKMDKCLSLSDVGLARIVVGCSNLNKISLKWCMEISDLGIDLLCKICKGLKSLDVSYL 209

Query: 289 -----SIR--------------DCPLIGDQGI-------ASLFSSTSYTLNKVKLQGL-- 348
                SIR               CPLI D G+        SL        ++V L GL  
Sbjct: 210 KITNDSIRSIALLVKLEVLDMVSCPLIDDGGLQFLENGSPSLQEVDVTRCDRVSLSGLIS 269

Query: 349 ---DVTDVSLAVIGHYGRAVTDLMLTGLTNVTE-RGFWVMGN----------GHGLQKLK 408
                 D+ L    H    V+   L  +  +   +  W+ G               + L 
Sbjct: 270 IVRGHPDIQLLKASHCVSEVSGSFLKYIKGLKHLKTIWIDGAHVSDSSLVSLSSSCRSLM 329

Query: 409 SLTLTSCHGVTDVGLQSIGKGCPNLRKFCLRKCSFLSDNGMVSFAQAAASIENLQLEECH 468
            + L+ C  VTD+G+ S+ + C NL+   L  C F++D  + + AQ+  ++  L+LE CH
Sbjct: 330 EIGLSRCVDVTDIGMISLARNCLNLKTLNLACCGFVTDVAISAVAQSCRNLGTLKLESCH 389

Query: 469 RITQLGLFGTILNCGAKL-KALSLVNCLGIKDLSLNLPSLTPCKSLQSLSICNCPGFGDA 528
            IT+ GL    L C + L + L L +C G+ D  L    ++ C +LQ L +  C    D 
Sbjct: 390 LITEKGL--QSLGCYSMLVQELDLTDCYGVNDRGLEY--ISKCSNLQRLKLGLCTNISDK 449

Query: 529 SLTLLSKLCPQLQHVNLSGLNGITDSGLLPLLKNCEAGLVKVNLSGCVNLTDKVISSLTK 588
            +  +   C +L  ++L    G  D GL  L + C++ L ++ LS C  LTD  +  + +
Sbjct: 450 GIFHIGSKCSKLLELDLYRCAGFGDDGLAALSRGCKS-LNRLILSYCCELTDTGVEQIRQ 509

Query: 589 LHGWTLEVLNLDGCSKVTDLSLVAIAENCPLLNDLDISKC-CISDFGIAALAQANQFNLQ 642
           L    L  L L G   +T + L AIA  C  L  LD+  C  I D G  ALA  ++ NL+
Sbjct: 510 LE--LLSHLELRGLKNITGVGLAAIASGCKKLGYLDVKLCENIDDSGFWALAYFSK-NLR 569

BLAST of Lag0004605 vs. TAIR 10
Match: AT5G01720.1 (RNI-like superfamily protein )

HSP 1 Score: 134.0 bits (336), Expect = 4.2e-31
Identity = 101/369 (27.37%), Postives = 164/369 (44.44%), Query Frame = 0

Query: 184 VTNIGLKAVAHGCPSLKAISLWNLSSIGDEGLIEIAKGCQLLEKLDLCQCPGISNKALLE 243
           V +  LK++ H C SLK +   +  ++   GL  +  G   L++LDL  C  + +     
Sbjct: 239 VDDDSLKSLRHDCKSLKKLDASSCQNLTHRGLTSLLSGAGYLQRLDLSHCSSVISLDFAS 298

Query: 244 LAKHCSNLTDITIESCANIGSESLQAIGQYCSNLKSISIRDCPLIGDQGIASLFSSTSYT 303
             K  S L  I ++ C ++  + L+AIG  C++LK +S+  C  + D+G++SL       
Sbjct: 299 SLKKVSALQSIRLDGC-SVTPDGLKAIGTLCNSLKEVSLSKCVSVTDEGLSSL------V 358

Query: 304 LNKVKLQGLDVT------DVSLAVIGHYGRAVTDLMLTGLTNVTERGFWVMG-------- 363
           +    L+ LD+T       VS+  I +    +  L +   + V+   FW++G        
Sbjct: 359 MKLKDLRKLDITCCRKLSRVSITQIANSCPLLVSLKMESCSLVSREAFWLIGQKCRLLEE 418

Query: 364 --------NGHGLQ------KLKSLTLTSCHGVTDVGLQSIGKGCPNLRKFCLRKCSFLS 423
                   +  GL+       L SL L  C  +TD GL  IG GC NLR+  L +   ++
Sbjct: 419 LDLTDNEIDDEGLKSISSCLSLSSLKLGICLNITDKGLSYIGMGCSNLRELDLYRSVGIT 478

Query: 424 DNGMVSFAQAAASIENLQLEECHRITQLGLFGTILNCGAKLKALSLVNCLGIKDLSLNLP 483
           D G+ + AQ    +E + +  C  IT   L    L+  + L+      C  I    L   
Sbjct: 479 DVGISTIAQGCIHLETINISYCQDITDKSLVS--LSKCSLLQTFESRGCPNITSQGLAAI 538

Query: 484 SLTPCKSLQSLSICNCPGFGDASLTLLSKLCPQLQHVNLSGLNGITDSGLLPLLK-NCEA 524
           ++  CK L  + +  CP   DA L  L+     L+ +N+S    +T+ GLL L    C  
Sbjct: 539 AVR-CKRLAKVDLKKCPSINDAGLLALAHFSQNLKQINVSD-TAVTEVGLLSLANIGCLQ 596

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
XP_038884618.10.0e+0089.84EIN3-binding F-box protein 1-like [Benincasa hispida][more]
KAA0064924.10.0e+0089.13EIN3-binding F-box protein 1 [Cucumis melo var. makuwa][more]
XP_023537957.10.0e+0089.53EIN3-binding F-box protein 1-like [Cucurbita pepo subsp. pepo][more]
XP_023002260.10.0e+0089.38EIN3-binding F-box protein 1-like [Cucurbita maxima][more]
XP_004138725.10.0e+0089.38EIN3-binding F-box protein 1 [Cucumis sativus] >KGN62950.1 hypothetical protein ... [more]
Match NameE-valueIdentityDescription
Q9SKK01.8e-21258.94EIN3-binding F-box protein 1 OS=Arabidopsis thaliana OX=3702 GN=EBF1 PE=1 SV=1[more]
Q708Y02.7e-19256.98EIN3-binding F-box protein 2 OS=Arabidopsis thaliana OX=3702 GN=EBF2 PE=1 SV=1[more]
Q9C5D21.8e-5027.47F-box/LRR-repeat protein 4 OS=Arabidopsis thaliana OX=3702 GN=FBL4 PE=2 SV=1[more]
Q9CZV82.4e-3127.48F-box/LRR-repeat protein 20 OS=Mus musculus OX=10090 GN=Fbxl20 PE=1 SV=3[more]
Q58DG65.4e-3127.25F-box/LRR-repeat protein 20 OS=Bos taurus OX=9913 GN=FBXL20 PE=2 SV=2[more]
Match NameE-valueIdentityDescription
A0A5A7VCC20.0e+0089.13EIN3-binding F-box protein 1 OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_sca... [more]
A0A6J1KNG40.0e+0089.38EIN3-binding F-box protein 1-like OS=Cucurbita maxima OX=3661 GN=LOC111496162 PE... [more]
A0A0A0LLZ60.0e+0089.38F-box domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_2G381760 PE=4 ... [more]
A0A6J1GHL00.0e+0088.91EIN3-binding F-box protein 1-like OS=Cucurbita moschata OX=3662 GN=LOC111454234 ... [more]
A0A1S3BBN90.0e+0089.66EIN3-binding F-box protein 1 OS=Cucumis melo OX=3656 GN=LOC103488312 PE=4 SV=1[more]
Match NameE-valueIdentityDescription
AT2G25490.11.3e-21358.94EIN3-binding F box protein 1 [more]
AT5G25350.11.9e-19356.98EIN3-binding F box protein 2 [more]
AT4G15475.11.3e-5127.47F-box/RNI-like superfamily protein [more]
AT5G27920.14.7e-3826.86F-box family protein [more]
AT5G01720.14.2e-3127.37RNI-like superfamily protein [more]
InterPro
Analysis Name: InterPro Annotations of Sponge gourd (AG-4) v1
Date Performed: 2022-08-01
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR006553Leucine-rich repeat, cysteine-containing subtypeSMARTSM00367LRR_CC_2coord: 222..247
e-value: 2.9E-4
score: 30.2
coord: 168..195
e-value: 86.0
score: 7.3
coord: 460..485
e-value: 0.0083
score: 25.3
coord: 592..617
e-value: 2.0
score: 17.4
coord: 248..273
e-value: 0.016
score: 24.4
coord: 380..405
e-value: 3.6E-4
score: 29.9
coord: 486..511
e-value: 5.7E-4
score: 29.2
coord: 274..299
e-value: 1.9E-4
score: 30.8
coord: 540..565
e-value: 17.0
score: 12.8
coord: 513..538
e-value: 8.6
score: 15.1
coord: 566..590
e-value: 0.25
score: 20.4
coord: 406..431
e-value: 500.0
score: 1.3
coord: 196..221
e-value: 5.6E-4
score: 29.2
coord: 354..379
e-value: 3.4E-4
score: 29.9
IPR001810F-box domainPFAMPF00646F-boxcoord: 73..112
e-value: 2.6E-6
score: 27.1
IPR001810F-box domainPROSITEPS50181FBOXcoord: 70..120
score: 8.808615
IPR001611Leucine-rich repeatPFAMPF13516LRR_6coord: 273..296
e-value: 0.088
score: 12.9
coord: 566..588
e-value: 0.25
score: 11.5
coord: 486..508
e-value: 0.27
score: 11.4
coord: 355..376
e-value: 0.06
score: 13.4
IPR032675Leucine-rich repeat domain superfamilyGENE3D3.80.10.10Ribonuclease Inhibitorcoord: 512..573
e-value: 5.2E-11
score: 44.2
coord: 455..511
e-value: 2.4E-11
score: 45.2
coord: 574..651
e-value: 1.3E-9
score: 39.6
IPR032675Leucine-rich repeat domain superfamilyGENE3D3.80.10.10Ribonuclease Inhibitorcoord: 66..300
e-value: 1.6E-34
score: 121.4
coord: 301..454
e-value: 3.9E-21
score: 77.3
NoneNo IPR availablePANTHERPTHR13318:SF135SUBFAMILY NOT NAMEDcoord: 22..642
NoneNo IPR availablePANTHERPTHR13318UNCHARACTERIZEDcoord: 22..642
NoneNo IPR availableSUPERFAMILY52047RNI-likecoord: 164..647
IPR036047F-box-like domain superfamilySUPERFAMILY81383F-box domaincoord: 69..110

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Lag0004605.1Lag0004605.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0031146 SCF-dependent proteasomal ubiquitin-dependent protein catabolic process
cellular_component GO:0019005 SCF ubiquitin ligase complex
molecular_function GO:0005515 protein binding