Lag0004547 (gene) Sponge gourd (AG‐4) v1

Overview


Name	Lag0004547
Type	gene
Organism	Luffa acutangula (Sponge gourd (AG‐4) v1)
Description	lariat debranching enzyme
Location	chr6: 4827753 .. 4830890 (-)
RNA-Seq Expression	Lag0004547
Synteny	Lag0004547

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: polypeptideexonCDS

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGAATAGGGGTTTAAATATACCTGCTCATGCTCCTAGGGTTTTTAGGAATTCAGAGGCGTTTCGGGACGAGCTAGGCGGAATCGAGGCGGCCAGAGGTAGTAGGGACCGAACGGAGGCGAATGAGCTCACCCAGCGCAAGCAGGCCGAGTTGGCCCTCAGTTTTCTGGTCTTATCCATGCCCGGTTGTCCTCGTCAGCTCCTTGTGCATGTGGTCCAAAAATACCTATAACATAACTTATAAACATATTGGGTGCCAAATTAGTTTATTAGTCTATCTATATAAGAAATTTTATTAAATCTTAACATCAATTCTCACAATAGGTACTAAAACTTTACAAATTTTGAAATACAAAGACTAAATTATAACATAGGAAAGTTTTAAGAACTAAATTATTACCAAATTAAAAACATAGGAACTAAATTGTTAGTTTTACGATTTCTCAAAAAAAAAAAAAAAATTACCAAAGAGACAAAAATGATTTTTAACTTATTTATTTATTATTAATGATATAATTAGTATTCCGAAAACAACGCTTGACAAAGGGGTTTTACTTCCATTCCTAGAAGAAAGAAATGCAGGGTTTATTTTTTTATTTTTTTATTTTTTATTTTTATTTTTTTTGATAGCATGCAGGGTTTATCTTTATGGGGTATGACTTGATTCATTTCATTGCAAATTGCCGGGTTGTTCTTAAATTCCAGCGATAACCTCGAAATCGTCTCTTGCGCGAAGAAGAAGAAGAAGCATGAGGATTGCAGTGGAAGGTTGCATGCACGGTGACCTCGACAATGTCTACAAAACTCTCCAATACATAGAGCAAGTTCAGAACATCAAAATCGACCTTCTACTCTGCTGTGGCGACTTTCAGGTAGTTCTTGAATTTTCCCGTTTCTTTTTCTATTTTTAAGTATCTACTCCCCAAAATCGTTACAATTTTCTGAGCAATTTAGGCTGTTAGAAATGAGAACGATCTGAAGAGCTTAAATGTGCCGCTGAAATATCGGTCCATGAACTCGTTCTGGAAGTATTACTCCGGAGCTGAAGTCGCTCCCTATCCTACTATTTTCATTGGTGGAAATCACGAAGCTTCTAATTATCTGTGGGAATTGTGAGTTACTTGAGCTTAGCTTGTGTTATGAGTTCTTCGGTCGTTTGTGTTTTTGGTGTATAGAGATATGAAATTTTCATTTATTTTGGGGGCAATAGAAATCTGAAAGTGAATGGAAGCAGATTCATAGATTACCGTTTGTGTATAGTTTTTAATTTTTCGAATGCTTTGAGTATTTGAGTAAGATGTCTCTATTGAATATTTGGTAATGAATGTTACCCGGAGAGCTATCGGATATTCAATATGCTCGATCGTTCTGTTGAGGAATTATATGAGATATTTTCAGAATCATCTCTATGCCCTTAATTTTAATTGTTTCCCGGTCCTTGTTTTCTTTTCTTTTCTTTTTTTTTTTCATTTATTTTTTTGTCTGAGGCTCTGTATTCTTATATGCATATGGTACATCAATAATTAGGATCTGATTTTATTTTAGGTACTATGGAGGTTGGGCTGCGCCTAATATATACTTCTTGGGCGTTGCTGGTGTAGTCAAGTTTGGAAATATCCGTATAGGTGGGCTGTCTGGGATTTATAATGCACGCCATTATCATTTAGGTGGGTGAATAACTTTCTGCCTTTTATTTATTTAGACGCAAAATGTTTAAGGTAGTTATCTGGATTGATTAGCATGCGGTCAAGGTTTCCTTTTGTTCCTTTCTTCGTGTGCCGAATTGGTCATGGAATCTGGTATACAAGAATGGCCTTGAGTGTGAATTAATAGCTTACAGAATGTGAAGCTACATCATCCATAGGTAGGTTTGTTCTGGGTTGTGGTTTTCTTTCTTGAGTGCCTTTGAGAAGACTCTAACATTTTATCATCTCTTAAGGAACTGTCTTATTGGTATATCTTTTGGACGTGGATTGATTGGTGGGTTAGTGTACTAATTCCTCATTAAATTTTAAGAATAGAGGAAGTCCTTCGTACTGGGAGTATGTTCATGATATTATGAAAAGTTGACATCTCAAATATAGTGTTGCACTTATCCATCTTTTTGTTGCCTGTTCAATATGCAATACAATAACTGTTGTGCTACTTACAACATAATCTTCTTCACGTGTAATGAGCAGGACACTTTGAGAGACCTCCATACGATGAGAATACTATAAGATCTGTTTACCATAAGATCTGTTTACCATGTTCGTGAATACGATGTTCAGAAGCTTATGCAAGTCGAGGAACCCATTGACATTTTTCTTTCCCATGATTGGCCACTTGGCATCACCGATTATGGAAACTGGAAACGACTCGTTAAGTCCAAACCATTTTTTGAGAAGGAGGTAGCTGTTATACTACCTGCTTACCCAACCATTTTCAAAAGTGCATGAATTTTCTACATGATATGAACTGCTTTAACATAGATGTATATTCTTTGATTGTGGCTTAGGTGTAAGATTTTTATTACTGTCATTTTGAGGACATCCAGCTCTGACATGAATTTGTGATTTCCATTTTTAAGGTCTTGATGAATGGTTTGTGTTATTACAGGTTATCCCCGAAATCCTCAGACTGAATCTTTGCTGAAGTTTCTTGAACTCCCTTATCTTCTTGATGACATGTTAGAATCTGCTGGACCATCTGGGAGTTCTATTCCTTTCTCAGATAGTGGTAAAACTATTTATCTTATGCTGTGGTTTATTTGCTGCTCGATAACTTCTGAGAATAAGTAGTTCTGTATCTGTTAATGATAGACGTAAATATCTTAGATCCATCTGGCCGTTGTTAGAAGTGAATGAAACTAAGAACTCTTAAGTATAGTTTTATTAGGACATATTGTTTTTTGTTCTTTGAATTTTACCATTAGCAGAATAGTATGGTTTTGTTACACTTCTCTAAGGTCTGTAATACTGCCCTGCCACACTGGATTTGGTGTATTGTATTAGTGCACGTGTTGTGAACTTACATTTCCCGAATAGCAAATTATTCAAACCCTCATAGTTGCAAGTTTTCTGAAATATTTATGGTTGTAGGTTCATTCGTCGACAATGAAGATATTCCAATTGATGATATAGATGATGAGGAAGAATAA

mRNA sequence

ATGAATAGGGGTTTAAATATACCTGCTCATGCTCCTAGGGTTTTTAGGAATTCAGAGGCGTTTCGGGACGAGCTAGGCGGAATCGAGGCGGCCAGAGGTAGTAGGGACCGAACGGAGGCGAATGAGCTCACCCAGCGCAAGCAGGCCGAGTTGGCCCTCAGTTTTCTGGTCTTATCCATGCCCGGTTGTCCTCCGATAACCTCGAAATCGTCTCTTGCGCGAAGAAGAAGAAGAAGCATGAGGATTGCAGTGGAAGGTTGCATGCACGGTGACCTCGACAATGTCTACAAAACTCTCCAATACATAGAGCAAGTTCAGAACATCAAAATCGACCTTCTACTCTGCTGTGGCGACTTTCAGGCTGTTAGAAATGAGAACGATCTGAAGAGCTTAAATGTGCCGCTGAAATATCGGTCCATGAACTCGTTCTGGAAGTATTACTCCGGAGCTGAAGTCGCTCCCTATCCTACTATTTTCATTGGTGGAAATCACGAAGCTTCTAATTATCTGTGGGAATTGTACTATGGAGGTTGGGCTGCGCCTAATATATACTTCTTGGGCGTTGCTGGTGTAGTCAAGTTTGGAAATATCCGTATAGGTGGGCTGTCTGGGATTTATAATGCACGCCATTATCATTTAGGACACTTTGAGAGACCTCCATACGATGAGAATACTATAAGATCTGTTTACCATAAGATCTGTTTACCATGTTATCCCCGAAATCCTCAGACTGAATCTTTGCTGAAGTTTCTTGAACTCCCTTATCTTCTTGATGACATGTTAGAATCTGCTGGACCATCTGGGAGTTCTATTCCTTTCTCAGATAGTGGTTCATTCGTCGACAATGAAGATATTCCAATTGATGATATAGATGATGAGGAAGAATAA

Coding sequence (CDS)

ATGAATAGGGGTTTAAATATACCTGCTCATGCTCCTAGGGTTTTTAGGAATTCAGAGGCGTTTCGGGACGAGCTAGGCGGAATCGAGGCGGCCAGAGGTAGTAGGGACCGAACGGAGGCGAATGAGCTCACCCAGCGCAAGCAGGCCGAGTTGGCCCTCAGTTTTCTGGTCTTATCCATGCCCGGTTGTCCTCCGATAACCTCGAAATCGTCTCTTGCGCGAAGAAGAAGAAGAAGCATGAGGATTGCAGTGGAAGGTTGCATGCACGGTGACCTCGACAATGTCTACAAAACTCTCCAATACATAGAGCAAGTTCAGAACATCAAAATCGACCTTCTACTCTGCTGTGGCGACTTTCAGGCTGTTAGAAATGAGAACGATCTGAAGAGCTTAAATGTGCCGCTGAAATATCGGTCCATGAACTCGTTCTGGAAGTATTACTCCGGAGCTGAAGTCGCTCCCTATCCTACTATTTTCATTGGTGGAAATCACGAAGCTTCTAATTATCTGTGGGAATTGTACTATGGAGGTTGGGCTGCGCCTAATATATACTTCTTGGGCGTTGCTGGTGTAGTCAAGTTTGGAAATATCCGTATAGGTGGGCTGTCTGGGATTTATAATGCACGCCATTATCATTTAGGACACTTTGAGAGACCTCCATACGATGAGAATACTATAAGATCTGTTTACCATAAGATCTGTTTACCATGTTATCCCCGAAATCCTCAGACTGAATCTTTGCTGAAGTTTCTTGAACTCCCTTATCTTCTTGATGACATGTTAGAATCTGCTGGACCATCTGGGAGTTCTATTCCTTTCTCAGATAGTGGTTCATTCGTCGACAATGAAGATATTCCAATTGATGATATAGATGATGAGGAAGAATAA

Protein sequence

MNRGLNIPAHAPRVFRNSEAFRDELGGIEAARGSRDRTEANELTQRKQAELALSFLVLSMPGCPPITSKSSLARRRRRSMRIAVEGCMHGDLDNVYKTLQYIEQVQNIKIDLLLCCGDFQAVRNENDLKSLNVPLKYRSMNSFWKYYSGAEVAPYPTIFIGGNHEASNYLWELYYGGWAAPNIYFLGVAGVVKFGNIRIGGLSGIYNARHYHLGHFERPPYDENTIRSVYHKICLPCYPRNPQTESLLKFLELPYLLDDMLESAGPSGSSIPFSDSGSFVDNEDIPIDDIDDEEE

Homology

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BLAST of Lag0004547 vs. NCBI nr
Match: XP_038886564.1 (lariat debranching enzyme isoform X1 [Benincasa hispida])

HSP 1 Score: 342.8 bits (878), Expect = 2.9e-90
Identity = 201/405 (49.63%), Postives = 209/405 (51.60%), Query Frame = 0


Query: 80  MRIAVEGCMHGDLDNVYKTLQYIEQVQNIKIDLLLCCGDFQAVRNENDLKSLNVPLKYRS 139
           MRIAVEGCMHGDLDNVY+TLQY+EQVQNIKIDLLLCCGDFQAVRNENDLKSLNVP KYRS
Sbjct: 1   MRIAVEGCMHGDLDNVYRTLQYMEQVQNIKIDLLLCCGDFQAVRNENDLKSLNVPPKYRS 60

Query: 140 MNSFWKYYSGAEVAPYPTIFIGGNHEASNYLWELYYGGWAAPNIYFLGVAGVVKFGNIRI 199
           MNSFWKYYSGAEVAPYPTIFIGGNHEASNYLWELYYGGWAAPNIYFLGVAGVVKFGNIRI
Sbjct: 61  MNSFWKYYSGAEVAPYPTIFIGGNHEASNYLWELYYGGWAAPNIYFLGVAGVVKFGNIRI 120

Query: 200 GGLSGIYNARHYHLGHFERPPYDENTIRSVYH---------------------------- 259
           GGLSGIYNARHYHLGH+ERPPY+ENTIRS+YH                            
Sbjct: 121 GGLSGIYNARHYHLGHYERPPYNENTIRSIYHVREYDVRKLMQVEEPIDIFLSHDWPLGI 180

Query: 260 ------------------------------------------------------------ 296
                                                                       
Sbjct: 181 TDYGDWKQLIRFKPFFEKEIQEKSLGSKAAAQLLEKLRPPYWFSAHLHCKFAALVQHGEG 240

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BLAST of Lag0004547 vs. NCBI nr
Match: XP_008445281.1 (PREDICTED: lariat debranching enzyme isoform X1 [Cucumis melo])

HSP 1 Score: 330.9 bits (847), Expect = 1.1e-86
Identity = 198/404 (49.01%), Postives = 205/404 (50.74%), Query Frame = 0


Query: 80  MRIAVEGCMHGDLDNVYKTLQYIEQVQNIKIDLLLCCGDFQAVRNENDLKSLNVPLKYRS 139
           MRIAVEGCMHGDLDNVY+TLQY+EQVQNIKIDLLLCCGDFQAVRNENDLKSLNV  KYRS
Sbjct: 1   MRIAVEGCMHGDLDNVYRTLQYMEQVQNIKIDLLLCCGDFQAVRNENDLKSLNVLPKYRS 60

Query: 140 MNSFWKYYSGAEVAPYPTIFIGGNHEASNYLWELYYGGWAAPNIYFLGVAGVVKFGNIRI 199
           MNSFWKYYSGAEVAPYPTIFIGGNHEASNYLWELYYGGWAAPNIYFLGVAGVVKFGNIRI
Sbjct: 61  MNSFWKYYSGAEVAPYPTIFIGGNHEASNYLWELYYGGWAAPNIYFLGVAGVVKFGNIRI 120

Query: 200 GGLSGIYNARHYHLGHFERPPYDENTIRSVYH---------------------------- 259
           GGLSGIYNARHYHLGH ERPPY+ENTIRS+YH                            
Sbjct: 121 GGLSGIYNARHYHLGHHERPPYNENTIRSIYHVREYDVQKLMQVEEPIDIFLSHDWPLGI 180

Query: 260 ------------------------------------------------------------ 295
                                                                       
Sbjct: 181 TDYGNWKQLVRFKPFFEKEIQERSLGSKAAAELLEKLKPPYWFSAHLHCKFAALVQHGEG 240

[Close]

BLAST of Lag0004547 vs. NCBI nr
Match: XP_011649851.1 (lariat debranching enzyme isoform X1 [Cucumis sativus] >KGN63007.1 hypothetical protein Csa_022405 [Cucumis sativus])

HSP 1 Score: 329.3 bits (843), Expect = 3.3e-86
Identity = 197/404 (48.76%), Postives = 203/404 (50.25%), Query Frame = 0


Query: 80  MRIAVEGCMHGDLDNVYKTLQYIEQVQNIKIDLLLCCGDFQAVRNENDLKSLNVPLKYRS 139
           MRIAVEGCMHGDLDNVY+TLQY+EQ QNIKIDLLLCCGDFQAVRNENDLKSLNVP KYRS
Sbjct: 1   MRIAVEGCMHGDLDNVYRTLQYMEQAQNIKIDLLLCCGDFQAVRNENDLKSLNVPPKYRS 60

Query: 140 MNSFWKYYSGAEVAPYPTIFIGGNHEASNYLWELYYGGWAAPNIYFLGVAGVVKFGNIRI 199
           MNSFWKYYSGAEVAPYPTIFIGGNHEASNYLWELYYGGWAAPNIYFLGVAGVVKFGNIRI
Sbjct: 61  MNSFWKYYSGAEVAPYPTIFIGGNHEASNYLWELYYGGWAAPNIYFLGVAGVVKFGNIRI 120

Query: 200 GGLSGIYNARHYHLGHFERPPYDENTIRSVYH---------------------------- 259
           GGLSGIYN RHYHLGH ERPPY+ENTIRS+YH                            
Sbjct: 121 GGLSGIYNERHYHLGHHERPPYNENTIRSIYHVREYDVQKLMQVEEPIDIFLSHDWPLGI 180

Query: 260 ------------------------------------------------------------ 295
                                                                       
Sbjct: 181 TDYGNWKQLVRFKPFFEKEIQEKSLGSKAAAVLLEKLKPPYWFSAHLHCKFAALVQHGEG 240

[Close]

BLAST of Lag0004547 vs. NCBI nr
Match: KAA0064874.1 (lariat debranching enzyme isoform X1 [Cucumis melo var. makuwa])

HSP 1 Score: 326.2 bits (835), Expect = 2.8e-85
Identity = 198/405 (48.89%), Postives = 205/405 (50.62%), Query Frame = 0


Query: 80  MRIAVEGCMHGDLDNVYKTLQYIEQVQNIKIDLLLCCGDFQAVRNENDLKSLNVPLKYRS 139
           MRIAVEGCMHGDLDNVY+TLQY+EQVQNIKIDLLLCCGDFQAVRNENDLKSLNV  KYRS
Sbjct: 1   MRIAVEGCMHGDLDNVYRTLQYMEQVQNIKIDLLLCCGDFQAVRNENDLKSLNVLPKYRS 60

Query: 140 MNSFWKYYSGAEVAPYPTIFIGGNHEASNYLWELYYGGWAAPNIYFLGVAGVVKFGNIRI 199
           MNSFWKYYSGAEVAPYPTIFIGGNHEASNYLWELYYGGWAAPNIYFLGVAGVVKFGNIRI
Sbjct: 61  MNSFWKYYSGAEVAPYPTIFIGGNHEASNYLWELYYGGWAAPNIYFLGVAGVVKFGNIRI 120

Query: 200 GGLSGIYNARHYHL-GHFERPPYDENTIRSVYH--------------------------- 259
           GGLSGIYNARHYHL GH ERPPY+ENTIRS+YH                           
Sbjct: 121 GGLSGIYNARHYHLAGHHERPPYNENTIRSIYHVREYDVQKLMQVEEPIDIFLSHDWPLG 180

Query: 260 ------------------------------------------------------------ 295
                                                                       
Sbjct: 181 ITDYGNWKQLVRFKPFFEKEIQERSLGSKAAAELLEKLKPPYWFSAHLHCKFAALVQHGE 240

[Close]

BLAST of Lag0004547 vs. NCBI nr
Match: XP_022132215.1 (lariat debranching enzyme [Momordica charantia])

HSP 1 Score: 324.7 bits (831), Expect = 8.2e-85
Identity = 196/404 (48.51%), Postives = 202/404 (50.00%), Query Frame = 0


Query: 80  MRIAVEGCMHGDLDNVYKTLQYIEQVQNIKIDLLLCCGDFQAVRNENDLKSLNVPLKYRS 139
           MRIAVEGCMHGDLDNVYKTLQ+IE+VQ IKIDLLLCCGDFQAVRNENDLKSLNVP KYRS
Sbjct: 1   MRIAVEGCMHGDLDNVYKTLQHIEKVQGIKIDLLLCCGDFQAVRNENDLKSLNVPPKYRS 60

Query: 140 MNSFWKYYSGAEVAPYPTIFIGGNHEASNYLWELYYGGWAAPNIYFLGVAGVVKFGNIRI 199
           MNSFWKYYSGAEVAPYPTIFIGGNHEASNYLWELYYGGWAAPNIYFLG AGVVKFGNIRI
Sbjct: 61  MNSFWKYYSGAEVAPYPTIFIGGNHEASNYLWELYYGGWAAPNIYFLGFAGVVKFGNIRI 120

Query: 200 GGLSGIYNARHYHLGHFERPPYDENTIRSVYH---------------------------- 259
           GGLSGIY AR+YHLGH ERPPY+ENTIRSVYH                            
Sbjct: 121 GGLSGIYKARNYHLGHHERPPYNENTIRSVYHVREYDVQKLMQLEEPIDIFLSHDWPVGI 180

Query: 260 ------------------------------------------------------------ 295
                                                                       
Sbjct: 181 TDYGDWEQLVRSKPFFEKEIQERSLGSKAAAELLEKLRPPYWFSAHLHCKFAALVQHGEG 240

[Close]

BLAST of Lag0004547 vs. ExPASy Swiss-Prot
Match: Q94K01 (Lariat debranching enzyme OS=Arabidopsis thaliana OX=3702 GN=DBR1 PE=2 SV=1)

HSP 1 Score: 280.0 bits (715), Expect = 3.0e-74
Identity = 138/214 (64.49%), Postives = 160/214 (74.77%), Query Frame = 0


Query: 80  MRIAVEGCMHGDLDNVYKTLQYIEQVQNIKIDLLLCCGDFQAVRNENDLKSLNVPLKYRS 139
           M+IA+EGCMHGDLDNVYKT+Q+ EQ+ N K+DLLLCCGDFQAVRNE D+ SLNVP KYR 
Sbjct: 1   MKIAIEGCMHGDLDNVYKTIQHYEQIHNTKVDLLLCCGDFQAVRNEKDMDSLNVPRKYRE 60

Query: 140 MNSFWKYYSGAEVAPYPTIFIGGNHEASNYLWELYYGGWAAPNIYFLGVAGVVKFGNIRI 199
           M SFWKYYSG EVAP PTIFIGGNHEASNYLWELYYGGWAA NIYFLG AGVVKFGN+RI
Sbjct: 61  MKSFWKYYSGQEVAPIPTIFIGGNHEASNYLWELYYGGWAATNIYFLGFAGVVKFGNVRI 120

Query: 200 GGLSGIYNARHYHLGHFERPPYDENTIRSVYHKICLPCYPRNPQTESLLKFLELPYLLDD 259
           GGLSGIYN RHY  GHFERPPY+E+TIRSVYH           +   + K ++L   LD 
Sbjct: 121 GGLSGIYNERHYRSGHFERPPYNESTIRSVYH----------VRDYDVQKLMQLEEPLDI 180

Query: 260 MLESAGPSGSSIPFSDSGSFVDNEDIPIDDIDDE 294
            L    P G +  + DS S +  +     +I+++
Sbjct: 181 FLSHDWPVGIT-DYGDSESLMRQKPYFRQEIEEK 203

[Close]

BLAST of Lag0004547 vs. ExPASy Swiss-Prot
Match: Q6AU07 (Lariat debranching enzyme OS=Oryza sativa subsp. japonica OX=39947 GN=DBR1 PE=2 SV=1)

HSP 1 Score: 277.3 bits (708), Expect = 2.0e-73
Identity = 124/152 (81.58%), Postives = 137/152 (90.13%), Query Frame = 0


Query: 80  MRIAVEGCMHGDLDNVYKTLQYIEQVQNIKIDLLLCCGDFQAVRNENDLKSLNVPLKYRS 139
           M+IAVEGCMHG+LD VY TL+ +E+ + +KIDLLLCCGDFQAVRNENDL+ LNV  ++R 
Sbjct: 1   MKIAVEGCMHGELDKVYDTLRELEKAEGVKIDLLLCCGDFQAVRNENDLQCLNVKPRFRE 60

Query: 140 MNSFWKYYSGAEVAPYPTIFIGGNHEASNYLWELYYGGWAAPNIYFLGVAGVVKFGNIRI 199
           M SFWKYYSG  VAPYPTIFIGGNHEASNYLWELYYGGWAAPNIYFLG AGVVKFGNIRI
Sbjct: 61  MKSFWKYYSGQAVAPYPTIFIGGNHEASNYLWELYYGGWAAPNIYFLGFAGVVKFGNIRI 120

Query: 200 GGLSGIYNARHYHLGHFERPPYDENTIRSVYH 232
           GGLSGIY  +HYHLGH+ERPPY+ENTIRSVYH
Sbjct: 121 GGLSGIYKQQHYHLGHYERPPYNENTIRSVYH 152

[Close]

BLAST of Lag0004547 vs. ExPASy Swiss-Prot
Match: Q6GPB8 (Lariat debranching enzyme A OS=Xenopus laevis OX=8355 GN=dbr1-a PE=2 SV=1)

HSP 1 Score: 232.3 bits (591), Expect = 7.3e-60
Identity = 100/152 (65.79%), Postives = 126/152 (82.89%), Query Frame = 0


Query: 80  MRIAVEGCMHGDLDNVYKTLQYIEQVQNIKIDLLLCCGDFQAVRNENDLKSLNVPLKYRS 139
           M+IAVEGC HG+LD +Y+T+Q++E+ +N K+DLLLCCGDFQAVRNE D+K + VP+KYR 
Sbjct: 1   MKIAVEGCCHGELDKIYETIQFLEKKENTKVDLLLCCGDFQAVRNEGDMKCMAVPMKYRQ 60

Query: 140 MNSFWKYYSGAEVAPYPTIFIGGNHEASNYLWELYYGGWAAPNIYFLGVAGVVKFGNIRI 199
           M +F+KYYSG + AP  TIFIGGNHEASNYL EL YGGW APNIY++G AGVVK+  +RI
Sbjct: 61  MQTFYKYYSGEKKAPILTIFIGGNHEASNYLQELPYGGWVAPNIYYMGYAGVVKYRGVRI 120

Query: 200 GGLSGIYNARHYHLGHFERPPYDENTIRSVYH 232
           GG+SGI+ +  Y  GHFERPPY ++T+RS YH
Sbjct: 121 GGISGIFKSHDYRKGHFERPPYSKDTVRSAYH 152

[Close]

BLAST of Lag0004547 vs. ExPASy Swiss-Prot
Match: Q6P886 (Lariat debranching enzyme OS=Xenopus tropicalis OX=8364 GN=dbr1 PE=2 SV=1)

HSP 1 Score: 231.5 bits (589), Expect = 1.2e-59
Identity = 100/152 (65.79%), Postives = 126/152 (82.89%), Query Frame = 0


Query: 80  MRIAVEGCMHGDLDNVYKTLQYIEQVQNIKIDLLLCCGDFQAVRNENDLKSLNVPLKYRS 139
           M+IAVEGC HG+LD +Y+T+Q++E+ +N K+DLLLCCGDFQAVRNE D+K + VP KYR 
Sbjct: 1   MKIAVEGCCHGELDKIYETIQFLEKKENTKVDLLLCCGDFQAVRNEGDMKCMAVPQKYRQ 60

Query: 140 MNSFWKYYSGAEVAPYPTIFIGGNHEASNYLWELYYGGWAAPNIYFLGVAGVVKFGNIRI 199
           M +F+KYYSG ++AP  TIFIGGNHEASNYL EL YGGW APNIY++G AGVVK+  +RI
Sbjct: 61  MQTFYKYYSGEKLAPILTIFIGGNHEASNYLQELPYGGWVAPNIYYVGYAGVVKYRGVRI 120

Query: 200 GGLSGIYNARHYHLGHFERPPYDENTIRSVYH 232
           GG+SGI+ +  Y  GHFERPPY ++T+RS YH
Sbjct: 121 GGISGIFKSHDYRKGHFERPPYSKDTVRSAYH 152

[Close]

BLAST of Lag0004547 vs. ExPASy Swiss-Prot
Match: Q7ZWU9 (Lariat debranching enzyme B OS=Xenopus laevis OX=8355 GN=dbr1-b PE=2 SV=1)

HSP 1 Score: 226.1 bits (575), Expect = 5.2e-58
Identity = 98/152 (64.47%), Postives = 125/152 (82.24%), Query Frame = 0


Query: 80  MRIAVEGCMHGDLDNVYKTLQYIEQVQNIKIDLLLCCGDFQAVRNENDLKSLNVPLKYRS 139
           M+IAVEGC HG+LD +Y+T+Q++E+ +N K++LLLCCGDFQAVRNE D+K + VP+KYR 
Sbjct: 1   MKIAVEGCCHGELDKIYETIQFLEKKENTKVELLLCCGDFQAVRNEGDMKCMAVPVKYRQ 60

Query: 140 MNSFWKYYSGAEVAPYPTIFIGGNHEASNYLWELYYGGWAAPNIYFLGVAGVVKFGNIRI 199
           M +F+KYYSG + AP  TIFIGGNHEASNYL EL YGGW APNIY++G AGVVK+  +RI
Sbjct: 61  MQTFYKYYSGEKKAPILTIFIGGNHEASNYLQELPYGGWVAPNIYYMGYAGVVKYRGVRI 120

Query: 200 GGLSGIYNARHYHLGHFERPPYDENTIRSVYH 232
           GG+SGI+ +  Y  GHFER PY ++T+RS YH
Sbjct: 121 GGISGIFKSHDYRKGHFERLPYGKDTVRSAYH 152

[Close]

BLAST of Lag0004547 vs. ExPASy TrEMBL
Match: A0A1S3BD21 (lariat debranching enzyme isoform X1 OS=Cucumis melo OX=3656 GN=LOC103488361 PE=3 SV=1)

HSP 1 Score: 330.9 bits (847), Expect = 5.5e-87
Identity = 198/404 (49.01%), Postives = 205/404 (50.74%), Query Frame = 0


Query: 80  MRIAVEGCMHGDLDNVYKTLQYIEQVQNIKIDLLLCCGDFQAVRNENDLKSLNVPLKYRS 139
           MRIAVEGCMHGDLDNVY+TLQY+EQVQNIKIDLLLCCGDFQAVRNENDLKSLNV  KYRS
Sbjct: 1   MRIAVEGCMHGDLDNVYRTLQYMEQVQNIKIDLLLCCGDFQAVRNENDLKSLNVLPKYRS 60

Query: 140 MNSFWKYYSGAEVAPYPTIFIGGNHEASNYLWELYYGGWAAPNIYFLGVAGVVKFGNIRI 199
           MNSFWKYYSGAEVAPYPTIFIGGNHEASNYLWELYYGGWAAPNIYFLGVAGVVKFGNIRI
Sbjct: 61  MNSFWKYYSGAEVAPYPTIFIGGNHEASNYLWELYYGGWAAPNIYFLGVAGVVKFGNIRI 120

Query: 200 GGLSGIYNARHYHLGHFERPPYDENTIRSVYH---------------------------- 259
           GGLSGIYNARHYHLGH ERPPY+ENTIRS+YH                            
Sbjct: 121 GGLSGIYNARHYHLGHHERPPYNENTIRSIYHVREYDVQKLMQVEEPIDIFLSHDWPLGI 180

Query: 260 ------------------------------------------------------------ 295
                                                                       
Sbjct: 181 TDYGNWKQLVRFKPFFEKEIQERSLGSKAAAELLEKLKPPYWFSAHLHCKFAALVQHGEG 240

[Close]

BLAST of Lag0004547 vs. ExPASy TrEMBL
Match: A0A0A0LQG6 (DBR1 domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_2G382800 PE=3 SV=1)

HSP 1 Score: 329.3 bits (843), Expect = 1.6e-86
Identity = 197/404 (48.76%), Postives = 203/404 (50.25%), Query Frame = 0


Query: 80  MRIAVEGCMHGDLDNVYKTLQYIEQVQNIKIDLLLCCGDFQAVRNENDLKSLNVPLKYRS 139
           MRIAVEGCMHGDLDNVY+TLQY+EQ QNIKIDLLLCCGDFQAVRNENDLKSLNVP KYRS
Sbjct: 1   MRIAVEGCMHGDLDNVYRTLQYMEQAQNIKIDLLLCCGDFQAVRNENDLKSLNVPPKYRS 60

Query: 140 MNSFWKYYSGAEVAPYPTIFIGGNHEASNYLWELYYGGWAAPNIYFLGVAGVVKFGNIRI 199
           MNSFWKYYSGAEVAPYPTIFIGGNHEASNYLWELYYGGWAAPNIYFLGVAGVVKFGNIRI
Sbjct: 61  MNSFWKYYSGAEVAPYPTIFIGGNHEASNYLWELYYGGWAAPNIYFLGVAGVVKFGNIRI 120

Query: 200 GGLSGIYNARHYHLGHFERPPYDENTIRSVYH---------------------------- 259
           GGLSGIYN RHYHLGH ERPPY+ENTIRS+YH                            
Sbjct: 121 GGLSGIYNERHYHLGHHERPPYNENTIRSIYHVREYDVQKLMQVEEPIDIFLSHDWPLGI 180

Query: 260 ------------------------------------------------------------ 295
                                                                       
Sbjct: 181 TDYGNWKQLVRFKPFFEKEIQEKSLGSKAAAVLLEKLKPPYWFSAHLHCKFAALVQHGEG 240

[Close]

BLAST of Lag0004547 vs. ExPASy TrEMBL
Match: A0A5A7VC81 (Lariat debranching enzyme isoform X1 OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold82G001870 PE=3 SV=1)

HSP 1 Score: 326.2 bits (835), Expect = 1.4e-85
Identity = 198/405 (48.89%), Postives = 205/405 (50.62%), Query Frame = 0


Query: 80  MRIAVEGCMHGDLDNVYKTLQYIEQVQNIKIDLLLCCGDFQAVRNENDLKSLNVPLKYRS 139
           MRIAVEGCMHGDLDNVY+TLQY+EQVQNIKIDLLLCCGDFQAVRNENDLKSLNV  KYRS
Sbjct: 1   MRIAVEGCMHGDLDNVYRTLQYMEQVQNIKIDLLLCCGDFQAVRNENDLKSLNVLPKYRS 60

Query: 140 MNSFWKYYSGAEVAPYPTIFIGGNHEASNYLWELYYGGWAAPNIYFLGVAGVVKFGNIRI 199
           MNSFWKYYSGAEVAPYPTIFIGGNHEASNYLWELYYGGWAAPNIYFLGVAGVVKFGNIRI
Sbjct: 61  MNSFWKYYSGAEVAPYPTIFIGGNHEASNYLWELYYGGWAAPNIYFLGVAGVVKFGNIRI 120

Query: 200 GGLSGIYNARHYHL-GHFERPPYDENTIRSVYH--------------------------- 259
           GGLSGIYNARHYHL GH ERPPY+ENTIRS+YH                           
Sbjct: 121 GGLSGIYNARHYHLAGHHERPPYNENTIRSIYHVREYDVQKLMQVEEPIDIFLSHDWPLG 180

Query: 260 ------------------------------------------------------------ 295
                                                                       
Sbjct: 181 ITDYGNWKQLVRFKPFFEKEIQERSLGSKAAAELLEKLKPPYWFSAHLHCKFAALVQHGE 240

[Close]

BLAST of Lag0004547 vs. ExPASy TrEMBL
Match: A0A6J1BRU1 (lariat debranching enzyme OS=Momordica charantia OX=3673 GN=LOC111005131 PE=3 SV=1)

HSP 1 Score: 324.7 bits (831), Expect = 4.0e-85
Identity = 196/404 (48.51%), Postives = 202/404 (50.00%), Query Frame = 0


Query: 80  MRIAVEGCMHGDLDNVYKTLQYIEQVQNIKIDLLLCCGDFQAVRNENDLKSLNVPLKYRS 139
           MRIAVEGCMHGDLDNVYKTLQ+IE+VQ IKIDLLLCCGDFQAVRNENDLKSLNVP KYRS
Sbjct: 1   MRIAVEGCMHGDLDNVYKTLQHIEKVQGIKIDLLLCCGDFQAVRNENDLKSLNVPPKYRS 60

Query: 140 MNSFWKYYSGAEVAPYPTIFIGGNHEASNYLWELYYGGWAAPNIYFLGVAGVVKFGNIRI 199
           MNSFWKYYSGAEVAPYPTIFIGGNHEASNYLWELYYGGWAAPNIYFLG AGVVKFGNIRI
Sbjct: 61  MNSFWKYYSGAEVAPYPTIFIGGNHEASNYLWELYYGGWAAPNIYFLGFAGVVKFGNIRI 120

Query: 200 GGLSGIYNARHYHLGHFERPPYDENTIRSVYH---------------------------- 259
           GGLSGIY AR+YHLGH ERPPY+ENTIRSVYH                            
Sbjct: 121 GGLSGIYKARNYHLGHHERPPYNENTIRSVYHVREYDVQKLMQLEEPIDIFLSHDWPVGI 180

Query: 260 ------------------------------------------------------------ 295
                                                                       
Sbjct: 181 TDYGDWEQLVRSKPFFEKEIQERSLGSKAAAELLEKLRPPYWFSAHLHCKFAALVQHGEG 240

[Close]

BLAST of Lag0004547 vs. ExPASy TrEMBL
Match: A0A6J1GKB9 (lariat debranching enzyme isoform X2 OS=Cucurbita moschata OX=3662 GN=LOC111454701 PE=3 SV=1)

HSP 1 Score: 322.0 bits (824), Expect = 2.6e-84
Identity = 196/405 (48.40%), Postives = 205/405 (50.62%), Query Frame = 0


Query: 80  MRIAVEGCMHGDLDNVYKTLQYIEQVQNIKIDLLLCCGDFQAVRNENDLKSLNVPLKYRS 139
           MRIAVEGCMHGDLDNVYKTLQY+EQVQNIKIDLLLCCGDFQAVRNE DLKSLNVP KYR+
Sbjct: 1   MRIAVEGCMHGDLDNVYKTLQYMEQVQNIKIDLLLCCGDFQAVRNEYDLKSLNVPPKYRA 60

Query: 140 MNSFWKYYSGAEVAPYPTIFIGGNHEASNYLWELYYGGWAAPNIYFLGVAGVVKFGNIRI 199
           MNSFWKYYSGAE+APYPTIFIGGNHEASNYLWELYYGGWAAPNIYFLGVAGVVKFGNIRI
Sbjct: 61  MNSFWKYYSGAEIAPYPTIFIGGNHEASNYLWELYYGGWAAPNIYFLGVAGVVKFGNIRI 120

Query: 200 GGLSGIYNARHYHLGHFERPPYDENTIRSVYH---------------------------- 259
           GGLSGIYNARHY LGH+ERPPY+E+TIRSVYH                            
Sbjct: 121 GGLSGIYNARHYRLGHYERPPYNEDTIRSVYHVREYDVQKLMQVEEPIDIFLSHDWPVGI 180

Query: 260 ------------------------------------------------------------ 296
                                                                       
Sbjct: 181 TDYGNWKQLVKFKPFFEKEIQERRLGSKAAAELLEKLKPPYWFSAHLHCKFAALVQHGEG 240

[Close]

BLAST of Lag0004547 vs. TAIR 10
Match: AT4G31770.1 (debranching enzyme 1 )

HSP 1 Score: 280.0 bits (715), Expect = 2.2e-75
Identity = 138/214 (64.49%), Postives = 160/214 (74.77%), Query Frame = 0


Query: 80  MRIAVEGCMHGDLDNVYKTLQYIEQVQNIKIDLLLCCGDFQAVRNENDLKSLNVPLKYRS 139
           M+IA+EGCMHGDLDNVYKT+Q+ EQ+ N K+DLLLCCGDFQAVRNE D+ SLNVP KYR 
Sbjct: 1   MKIAIEGCMHGDLDNVYKTIQHYEQIHNTKVDLLLCCGDFQAVRNEKDMDSLNVPRKYRE 60

Query: 140 MNSFWKYYSGAEVAPYPTIFIGGNHEASNYLWELYYGGWAAPNIYFLGVAGVVKFGNIRI 199
           M SFWKYYSG EVAP PTIFIGGNHEASNYLWELYYGGWAA NIYFLG AGVVKFGN+RI
Sbjct: 61  MKSFWKYYSGQEVAPIPTIFIGGNHEASNYLWELYYGGWAATNIYFLGFAGVVKFGNVRI 120

Query: 200 GGLSGIYNARHYHLGHFERPPYDENTIRSVYHKICLPCYPRNPQTESLLKFLELPYLLDD 259
           GGLSGIYN RHY  GHFERPPY+E+TIRSVYH           +   + K ++L   LD 
Sbjct: 121 GGLSGIYNERHYRSGHFERPPYNESTIRSVYH----------VRDYDVQKLMQLEEPLDI 180

Query: 260 MLESAGPSGSSIPFSDSGSFVDNEDIPIDDIDDE 294
            L    P G +  + DS S +  +     +I+++
Sbjct: 181 FLSHDWPVGIT-DYGDSESLMRQKPYFRQEIEEK 203

The following BLAST results are available for this feature:

BLAST of Lag0004547 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_038886564.1	2.9e-90	49.63	lariat debranching enzyme isoform X1 [Benincasa hispida]	[more]
XP_008445281.1	1.1e-86	49.01	PREDICTED: lariat debranching enzyme isoform X1 [Cucumis melo]	[more]
XP_011649851.1	3.3e-86	48.76	lariat debranching enzyme isoform X1 [Cucumis sativus] >KGN63007.1 hypothetical ...	[more]
KAA0064874.1	2.8e-85	48.89	lariat debranching enzyme isoform X1 [Cucumis melo var. makuwa]	[more]
XP_022132215.1	8.2e-85	48.51	lariat debranching enzyme [Momordica charantia]	[more]

BLAST of Lag0004547 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
Q94K01	3.0e-74	64.49	Lariat debranching enzyme OS=Arabidopsis thaliana OX=3702 GN=DBR1 PE=2 SV=1	[more]
Q6AU07	2.0e-73	81.58	Lariat debranching enzyme OS=Oryza sativa subsp. japonica OX=39947 GN=DBR1 PE=2 ...	[more]
Q6GPB8	7.3e-60	65.79	Lariat debranching enzyme A OS=Xenopus laevis OX=8355 GN=dbr1-a PE=2 SV=1	[more]
Q6P886	1.2e-59	65.79	Lariat debranching enzyme OS=Xenopus tropicalis OX=8364 GN=dbr1 PE=2 SV=1	[more]
Q7ZWU9	5.2e-58	64.47	Lariat debranching enzyme B OS=Xenopus laevis OX=8355 GN=dbr1-b PE=2 SV=1	[more]

BLAST of Lag0004547 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A1S3BD21	5.5e-87	49.01	lariat debranching enzyme isoform X1 OS=Cucumis melo OX=3656 GN=LOC103488361 PE=...	[more]
A0A0A0LQG6	1.6e-86	48.76	DBR1 domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_2G382800 PE=3 S...	[more]
A0A5A7VC81	1.4e-85	48.89	Lariat debranching enzyme isoform X1 OS=Cucumis melo var. makuwa OX=1194695 GN=E...	[more]
A0A6J1BRU1	4.0e-85	48.51	lariat debranching enzyme OS=Momordica charantia OX=3673 GN=LOC111005131 PE=3 SV...	[more]
A0A6J1GKB9	2.6e-84	48.40	lariat debranching enzyme isoform X2 OS=Cucurbita moschata OX=3662 GN=LOC1114547...	[more]

BLAST of Lag0004547 vs. TAIR 10


Match Name	E-value	Identity	Description
AT4G31770.1	2.2e-75	64.49	debranching enzyme 1	[more]

InterPro

Analysis Name: InterPro Annotations of Sponge gourd (AG-4) v1
Date Performed: 2022-08-01

Position :

Zoom :

x 1


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR029052	Metallo-dependent phosphatase-like	GENE3D	3.60.21.10		coord: 80..256 e-value: 1.3E-5 score: 26.8
IPR029052	Metallo-dependent phosphatase-like	SUPERFAMILY	56300	Metallo-dependent phosphatases	coord: 79..223
IPR004843	Calcineurin-like phosphoesterase domain, ApaH type	PFAM	PF00149	Metallophos	coord: 80..217 e-value: 8.2E-9 score: 36.2
None	No IPR available	MOBIDB_LITE	mobidb-lite	disorder_prediction	coord: 267..295
None	No IPR available	PANTHER	PTHR12849	RNA LARIAT DEBRANCHING ENZYME	coord: 80..231

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
Lag0004547.1	Lag0004547.1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0009793	embryo development ending in seed dormancy
biological_process	GO:0006397	mRNA processing
biological_process	GO:0090502	RNA phosphodiester bond hydrolysis, endonucleolytic
cellular_component	GO:0005634	nucleus
molecular_function	GO:0008419	RNA lariat debranching enzyme activity
molecular_function	GO:0016787	hydrolase activity