Lag0004462 (gene) Sponge gourd (AG‐4) v1

Overview
NameLag0004462
Typegene
OrganismLuffa acutangula (Sponge gourd (AG‐4) v1)
DescriptiontRNA (adenine(58)-N(1))-methyltransferase non-catalytic subunit TRM6
Locationchr6: 4129839 .. 4135766 (-)
RNA-Seq ExpressionLag0004462
SyntenyLag0004462
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: polypeptideexonCDS
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGTCCTCGAGTATTCTTCCATCTGAATCGCCTCGAAACCCTAGAGTGACGTGGGAGGGCTGCAGCGTTCTGCTCGACATCAATGATGGCGACCGCCTGGTTTTTGCTCGACTCTCGCTCGCCTCGTACGTCTTTCGCTCCCAAATTTCAGTGTAGATTTCTGGTATTTAATGTTCAGTAAGTATGATGCGTGATAGCAAGAATTACTTTGATTTGCAGTACGCTGAAAATTGGGAATAAAAGTTACTCTCTAAAGCCTTTGATCGGTTGTCCATTCGGTTCCTTGTTTCAAATTGAAAATGGAGCCAATGGTCCACATTTGGCTCGCGTTATGCCCTCCAGAGAAGAAGGTTTGATTTCTTACTGCGGATTTTGTTAACGAATTGGTTTCATCTTTACAAAACTTTTAACTGAAGCGATCTTTTGTTTTGGTGTCTGTTTCCGAAGGCATTAACTGTCAAGAAACGGAGGAGATGAAGGAAGAGCAGGTGACAAAAGATGAGCTGAGAGATAACAGAGCGATTGTAGACAATAATAAGGCACAAAGCCTCTCTAGTGAAGATATCGATGCAATGCGAAGGTAAGATATTGCTCTGCTTTTTCCACTGGTTCAAGAACAAGTTGAGGCTTAAGCATCTTTTCAGATTGTTTACACCTCCTCGCCTTATTCGTTTGAAGCATACACCTCATTTAAATTATAAGAGGTTTACAGCTCCTACAGAGGCATACTCAGACTCACAGTCTTTAGTCCCTTCCAAGTAAGGAGATATAAGGAGAAGATATGTGGGGTGTCCCATAGTCCCTTACAAGTGGTATTGGAGTCATGCCCTTGACGTAGCCGGGTGTCGAACAAAGGATTTGTGAGCCTTAAAAACATAGTCTTGGTGGAGGATAGATGATTATTTGAGGGAGGCTTGATAGTTTACTTTGTTCGAGGGGAGATTGTTGGGAATTGGGGAGTGCGACCAAAGTCCCACATTTGGCTAGATAAGGGGATGATCATGGGTAAGGGAGGGCAAATATCTCCATTGGTTTGAGGCCTTTTGGGGTAAACCAAAAACAAAGCCATGAGGGTTTATGCCCAAAGTGGACAATATCATACCATTGTGGAGATTTGTGTCGTGTTCTCGTCCTTAACAAGAAGATTCATGTAATAGTTAGAAGATTAATAAAGAAAAAAAAATACTGGTGATAATAATGAAAATGTTGAAAATAACCTTTGAAAATTTTATTTTGTTACAGAAAATAATAATTAATAGACGTGATTTTTTTTTTCCCTTTTTGTACTTTTGTAAAATATAATCGGTGATTCGTATAGTTTGTTTAGTCATGTGTCTCAGACTGCCTTTAACCTTTTAAAACATGTTCCAAACACATGTTTGTCACAACAGATGAAGTCACAAAATCTTGAATTAACCAGTGATGAATGCTTTGGCAATTTTAGATCTTATTTCTATGCAGGATATATTAGGCATCACATTATTATATGACTATTATTATTATTATTTGAACTGGTTGGTGACATAATAGGAGCAAACGAACTTTATCTTTTCTTCGTCAAACGTGGCAGCACAATGAACTTGCTTGAACCTTGCCTCTGTCGCTGTGATTCTAGCCCTGTGGCATCCCTCTTGCTTGAACCTGTTCCTCCCATTCTTTTCATTGTTTTGACATTTATGAGAATTTAAGTTTAATTTATTTTATTTTGTGAAACTTGCCACCGTGTTTTTCATAAATATGTTATTATCCATGATGCATACTCTTTCTCTTTATATGTAGTCAGGGTGCAACTGGTGATGAAATTGTGGAAGCTCTCATTGCCAACAGTGCAACATTTGAAAAGAAAACAGTTTTCTCTCAGGTGATCAGAATGTGCATATACTGAGTAGTTTCATCGTTGTTTGTTGTCTGGCATTTTTTTTTCTAAAGTTGACCAGTTTATTTTATCTGCAGGAGAAATATAGGCTCAGGAAACAGAAGAAGTATGCTCCTAGGGTACTTTTGAGGAGACCTTTTACTCGAAGGTATCCATGATAGCGCTGTGTTAACTGTTACCAAGAATTTTAATATATGGACTCAAGAAAACTAATGATAGTTTATATCACAATCATGAATATTTGTATTTGTAACATCACTAGAATTCACATATTTTATGCTGTCTAGCTTCTATTATTTATTGCTGATGTTTTTTCCCCATCTTACAGTATATGTGAGGCATACTTCAAGAAATATCCAGCTAGAATTGGGTAATTTTATTTGTCCACTTTTTCACTACATTTTTGTCCATTTAGTTTTAATTCTTTCCATAGAGTGTGCTTCTCGTCTTTTCTAAAAAATCATTTATTTTAGTTGTAACAATCTGCATGTTTTCTATTCATTAAATGACTATTTGCCTCAGTTTTTTGGCCTTTCGGAAGTATTACCGTGATAATACTTTTCTGATCTTTCTTGCACCTCATGACTTGTTTAAGGAATAGTTGAAATTTTTTCCAGCAATATCACAATTCACAACCTAATATGCAAGTTCAATTATAATGTTTTATAGGTAACAGCATTTTTTTTGCCCTTGCCATTTATTTCTTGGTCTGTCTCCGTTAGGAACCAAAGCAGTATGGGATGCCTTTGTCCCACATTGGAAAGATATGGGCTTCTAATGGGGTATATAAGTGGTTGGTTCTCCCTCCCTAATCGCCTGGCTTTTGGGGTGTGGCTCTCCAACTACTTAGATACCTAACAATTGGTATCAGAGCCGGTGGTCGACGGCTTCGGAGAGGAGTGGCGGCGGAGGGGCTGACCGAACCAGCCGAGTGAAGCGTCGCCGTGCACAGCCGCTGAGACGTCGGATTTCCGGGGAGGGGTAATTGTTAGGGACCAAGGCAGTATGGGATGCCTTTGTCCCACATTGGAAAGATATGAGCTTCTAATGGGGTATATAAGTGGTTGGTTCTCCCTCCCCAATCGCCTGGCTTTTGGGGTGTGGCTCTCCAACTACTTAGATACCTAACAGTCTCCTGGCGCACTGTGTGATAATTGAATTTCACATTTTTGAATATCTTTTTTTTTTTTTGAAATAACATTATTGAATATCTTCAAGTTTCTATTCTTTCCTTTTTTTCATTACTCATCATAGCTCTCTGATGGTTTGATGTTGGGGAACAGTATTAATGTAGTCTGTTTCCTTTCTTTGTGATTCAGATTCTTGCGAGTGGATGCTTTATCTCTTCTTCTTTCTTTTGCCAACGTGACTGCAAATTCTGATGTACTTGTGTTTGATATGGTTGGTGGTCTCCTCACTGGAGCCGTGGCTGAGCGCTTAGGAGGTATGTCATATCCTCTATTATTCTTGGCATTAATAAAGTGATAGTGATGGTTCATTTTTGTTAAAAAAAATGGCCTTGTAAATCTGGATGAATGTGAGGCATGGTATAAAAGCAGAGGTGTATGAGCAGATGCTGACAGCCTCTTTGCACTTTTCTCAAGCAAATTAGGGTCTTACAGTGTATGCATATAGTTTCTATGTCATTAACCATATTTCCAGTCTTGCTATTTCCCAAATTCAATTTTGAATTGTGTTAGTGTACCAAACAATGTTCGGTGTCCATCCAAGATGCTCTGGTAGGTTAGGGCTTGAAATATTTGGAGGTTCAAGGTCGTGAGTGTTTTACCATGTTGAAACTTAATGACTTAAAACCCATGAAACATGTTGTTTCTAGAGTTAGTCGTGGATGGCAAAAAGCCTCTGTCAGATATCAGTGTGAGTGGTCCATAGGCATAATTCAAATAAACCGGGGAAAAAACATTGTTGTTAGGTGTCAGGTTGCAATACGATAAGTACAATCGTGCGTTCTGTTCTACAATATATGATGCTAAATTGTGTTGGTTTTTTACAGGCAAGGGTTCTGTATGCAATACATACCTTGGAAATAATCCAAATCCTATGGATATAGTAAGAATATTCAACTTTGATGATGAAATTTGCGCGAGGTTTGCTGCTTTGGTTCCTCTTTCTTATATTGTTTTATTGTTTGCATTACCCATTTTTTAAATGTCTTTCTAGTTTCTACCCCATTCTCTAACTGTTTATCCACCAGGATAATGCGTTCGCCTCTTGTTGACCTTGCCTCATCCCAAGTTCAACCTTCCAAATCTAATGGTGAACATGAAGCCGATGTAAGTAAAGTTTTTCTTTTTCAATGTTTTCTTAAGATGAGTTTCGAATTGACTTTTGGGATGCTGAAGAGTGCTTTTGACCAAGTGCTTATAAGATTTTTGGAAGCATTTGGTGTATAACCAAAAGTGATTTTGAAATTCCTAAAAATACTTTAGAAAGGACTTTATAATTTGTTTAGGAGAAGCCTTTAATTAGTCCTCCCACAAGTACCAGTCAAAAATTGTTATTCTCAAAATTCATTATTTTGGTTGGATGGTACTAATTTATTTTATTTTATCTTATTGGTATAAGATTAATGCACGGGATCAGGCTTCTGAACCTGTTAGTATGGATGAAAGCCCTCTTCCATCTACAGACCAGTCCTCAGATCTCAACTCAGAGTCTATAGTTTCTCCATTAACAAAAGTAGTTAAATGCTCAAAGGCTGGAGATAAGGCACCGCCAGAAGTTGTTAAATTATGGAAGGAAAATGGTTTTACAAGGTGAGGAGTATGGTTTGGTGGTAATATGTGCTTCACCATTAATAGTAATTCAATTTGATTATTTTGAATAATTGCAGCCTAATAATTGCTGCACCACAGTCGGATACATGGAGTCTTGTTAAAGATCTGTTGCCTCTTGTAGCAAACTCTGCCCCATTTGCCATCTATCACCAGTATCTTCAGGTTGGTTCTTTGTAAAGTTTACCTTCTCAACTTCTATCTTTTCCATACTACTCTCACTCGGAGGAGTGCCAAGGGCTTAAAGATGTTTGAAGATGAGCACAAAAGTCTTGATTATAATAGAACATATTTTAATGGGTGCAATGCATCGTTTTCCATCAAAACTCCATAGTTAAGCATGTTTGGACGAGAGTAATAAGTTGGGTGATCAATTGGGAAAAAACAGATAACTTTTTTTTTTTTTTTTTTTTTTGATAAGAAACAATTTCATTGATGTATGAAATTTACAAAAGGAATACAAGATCCGTAGGAGTTACTGAAGGCTTTCCCAACTATTGATGAGGGAAAAAACAGATATTATTATTATTATTATTTTCCAAATGGATCCAAATAGAAATGTGATGTCATGTGATGGGGCAGTGATTGTAAATCGATGTTATATATATTGATTAAGTTGATCATTTGTTAAATGCAGCCTCTTGCAACTTGCATGCATAAACTACAGCTTGAGAAGATGGCAATCAGCTTGCAAATATCAGAACCTTGGCTACGAGAATACCAGGTTTGATGGCTCTACAGTTCCGCACACTTCCCTTCTAACAAGTTTTAGGAATTGTCATATAGAAGATAATGATCTGAATTTAAAGGATTCATACTCCTAATATTACCCTTCCAATATCATAACGAGGGATTTATGCTTTTTTCTTTTTTTTAGCTAAATTTATGAATATTTTAGTCTTAGAGAATATTATAAAGTTAGTCTAAATTTAAAAGTTGGTTTGACTTATTGGTTACTCGTGGAAGTGAGAAAAAGTAAAGATTTTTGTAGAAATTCCATGGTGGCAACCAATTTAGGATATCTGAATGGCTGGATCATCATAATATATACAAATATAAATCACTTTGTGGTTTGGTTGGAAGGGATCGTATTTTCTATATATAAAGAAAAATTAAGTTACTCTAAATTTATAAAATAGATGTGACAATTTTATCATATTAATGCAGGTTCTTCCTTCGAGAACCCATCCCTTCATGCAGATGAATGCATTTGGTGGATATATTCTTAGTGGGACGAAGATATGTGGCCATCAATAG

mRNA sequence

ATGTCCTCGAGTATTCTTCCATCTGAATCGCCTCGAAACCCTAGAGTGACGTGGGAGGGCTGCAGCGTTCTGCTCGACATCAATGATGGCGACCGCCTGGTTTTTGCTCGACTCTCGCTCGCCTCTACGCTGAAAATTGGGAATAAAAGTTACTCTCTAAAGCCTTTGATCGGTTGTCCATTCGGTTCCTTGTTTCAAATTGAAAATGGAGCCAATGGTCCACATTTGGCTCGCGTTATGCCCTCCAGAGAAGAAGGCATTAACTGTCAAGAAACGGAGGAGATGAAGGAAGAGCAGGTGACAAAAGATGAGCTGAGAGATAACAGAGCGATTGTAGACAATAATAAGGCACAAAGCCTCTCTAGTGAAGATATCGATGCAATGCGAAGTCAGGGTGCAACTGGTGATGAAATTGTGGAAGCTCTCATTGCCAACAGTGCAACATTTGAAAAGAAAACAGTTTTCTCTCAGGAGAAATATAGGCTCAGGAAACAGAAGAAGTATGCTCCTAGGGTACTTTTGAGGAGACCTTTTACTCGAAGTATATGTGAGGCATACTTCAAGAAATATCCAGCTAGAATTGGATTCTTGCGAGTGGATGCTTTATCTCTTCTTCTTTCTTTTGCCAACGTGACTGCAAATTCTGATGTACTTGTGTTTGATATGGTTGGTGGTCTCCTCACTGGAGCCGTGGCTGAGCGCTTAGGAGGCAAGGGTTCTGTATGCAATACATACCTTGGAAATAATCCAAATCCTATGGATATAGTAAGAATATTCAACTTTGATGATGAAATTTGCGCGAGGATAATGCGTTCGCCTCTTGTTGACCTTGCCTCATCCCAAGTTCAACCTTCCAAATCTAATGGTGAACATGAAGCCGATATTAATGCACGGGATCAGGCTTCTGAACCTGTTAGTATGGATGAAAGCCCTCTTCCATCTACAGACCAGTCCTCAGATCTCAACTCAGAGTCTATAGTTTCTCCATTAACAAAAGTAGTTAAATGCTCAAAGGCTGGAGATAAGGCACCGCCAGAAGTTGTTAAATTATGGAAGGAAAATGGTTTTACAAGCCTAATAATTGCTGCACCACAGTCGGATACATGGAGTCTTGTTAAAGATCTGTTGCCTCTTGTAGCAAACTCTGCCCCATTTGCCATCTATCACCAGTATCTTCAGCCTCTTGCAACTTGCATGCATAAACTACAGCTTGAGAAGATGGCAATCAGCTTGCAAATATCAGAACCTTGGCTACGAGAATACCAGGTTCTTCCTTCGAGAACCCATCCCTTCATGCAGATGAATGCATTTGGTGGATATATTCTTAGTGGGACGAAGATATGTGGCCATCAATAG

Coding sequence (CDS)

ATGTCCTCGAGTATTCTTCCATCTGAATCGCCTCGAAACCCTAGAGTGACGTGGGAGGGCTGCAGCGTTCTGCTCGACATCAATGATGGCGACCGCCTGGTTTTTGCTCGACTCTCGCTCGCCTCTACGCTGAAAATTGGGAATAAAAGTTACTCTCTAAAGCCTTTGATCGGTTGTCCATTCGGTTCCTTGTTTCAAATTGAAAATGGAGCCAATGGTCCACATTTGGCTCGCGTTATGCCCTCCAGAGAAGAAGGCATTAACTGTCAAGAAACGGAGGAGATGAAGGAAGAGCAGGTGACAAAAGATGAGCTGAGAGATAACAGAGCGATTGTAGACAATAATAAGGCACAAAGCCTCTCTAGTGAAGATATCGATGCAATGCGAAGTCAGGGTGCAACTGGTGATGAAATTGTGGAAGCTCTCATTGCCAACAGTGCAACATTTGAAAAGAAAACAGTTTTCTCTCAGGAGAAATATAGGCTCAGGAAACAGAAGAAGTATGCTCCTAGGGTACTTTTGAGGAGACCTTTTACTCGAAGTATATGTGAGGCATACTTCAAGAAATATCCAGCTAGAATTGGATTCTTGCGAGTGGATGCTTTATCTCTTCTTCTTTCTTTTGCCAACGTGACTGCAAATTCTGATGTACTTGTGTTTGATATGGTTGGTGGTCTCCTCACTGGAGCCGTGGCTGAGCGCTTAGGAGGCAAGGGTTCTGTATGCAATACATACCTTGGAAATAATCCAAATCCTATGGATATAGTAAGAATATTCAACTTTGATGATGAAATTTGCGCGAGGATAATGCGTTCGCCTCTTGTTGACCTTGCCTCATCCCAAGTTCAACCTTCCAAATCTAATGGTGAACATGAAGCCGATATTAATGCACGGGATCAGGCTTCTGAACCTGTTAGTATGGATGAAAGCCCTCTTCCATCTACAGACCAGTCCTCAGATCTCAACTCAGAGTCTATAGTTTCTCCATTAACAAAAGTAGTTAAATGCTCAAAGGCTGGAGATAAGGCACCGCCAGAAGTTGTTAAATTATGGAAGGAAAATGGTTTTACAAGCCTAATAATTGCTGCACCACAGTCGGATACATGGAGTCTTGTTAAAGATCTGTTGCCTCTTGTAGCAAACTCTGCCCCATTTGCCATCTATCACCAGTATCTTCAGCCTCTTGCAACTTGCATGCATAAACTACAGCTTGAGAAGATGGCAATCAGCTTGCAAATATCAGAACCTTGGCTACGAGAATACCAGGTTCTTCCTTCGAGAACCCATCCCTTCATGCAGATGAATGCATTTGGTGGATATATTCTTAGTGGGACGAAGATATGTGGCCATCAATAG

Protein sequence

MSSSILPSESPRNPRVTWEGCSVLLDINDGDRLVFARLSLASTLKIGNKSYSLKPLIGCPFGSLFQIENGANGPHLARVMPSREEGINCQETEEMKEEQVTKDELRDNRAIVDNNKAQSLSSEDIDAMRSQGATGDEIVEALIANSATFEKKTVFSQEKYRLRKQKKYAPRVLLRRPFTRSICEAYFKKYPARIGFLRVDALSLLLSFANVTANSDVLVFDMVGGLLTGAVAERLGGKGSVCNTYLGNNPNPMDIVRIFNFDDEICARIMRSPLVDLASSQVQPSKSNGEHEADINARDQASEPVSMDESPLPSTDQSSDLNSESIVSPLTKVVKCSKAGDKAPPEVVKLWKENGFTSLIIAAPQSDTWSLVKDLLPLVANSAPFAIYHQYLQPLATCMHKLQLEKMAISLQISEPWLREYQVLPSRTHPFMQMNAFGGYILSGTKICGHQ
Homology
BLAST of Lag0004462 vs. NCBI nr
Match: XP_038884150.1 (tRNA (adenine(58)-N(1))-methyltransferase non-catalytic subunit trm6 [Benincasa hispida])

HSP 1 Score: 827.4 bits (2136), Expect = 6.0e-236
Identity = 420/451 (93.13%), Postives = 433/451 (96.01%), Query Frame = 0

Query: 1   MSSSILPSESPRNPRVTWEGCSVLLDINDGDRLVFARLSLASTLKIGNKSYSLKPLIGCP 60
           MSSSIL SESPRNPR+TWEGCSVLLD+NDGDRLVFARLS ASTLKIGNKS+SLKPLIGCP
Sbjct: 1   MSSSILQSESPRNPRLTWEGCSVLLDVNDGDRLVFARLSPASTLKIGNKSFSLKPLIGCP 60

Query: 61  FGSLFQIENGANGPHLARVMPSREEGINCQETEEMKEEQVTKDELRDNRAIVDNNKAQSL 120
           FGSLFQIENG NGPHLARVMPSREEG NCQETEEMKEEQVTKDELRDNRAIVDNNKAQSL
Sbjct: 61  FGSLFQIENGGNGPHLARVMPSREEGNNCQETEEMKEEQVTKDELRDNRAIVDNNKAQSL 120

Query: 121 SSEDIDAMRSQGATGDEIVEALIANSATFEKKTVFSQEKYRLRKQKKYAPRVLLRRPFTR 180
           SS+DIDAMRSQGATGDEIVEALIANSATFEKKTVFSQEKYRLRKQKKYAPRVLLRRPFTR
Sbjct: 121 SSDDIDAMRSQGATGDEIVEALIANSATFEKKTVFSQEKYRLRKQKKYAPRVLLRRPFTR 180

Query: 181 SICEAYFKKYPARIGFLRVDALSLLLSFANVTANSDVLVFDMVGGLLTGAVAERLGGKGS 240
           SICEAYFKKYPARIGFLR+DALSLLLSFANVTA+SDVLV DMVGGLLTGAVAERLGGKGS
Sbjct: 181 SICEAYFKKYPARIGFLRLDALSLLLSFANVTAHSDVLVVDMVGGLLTGAVAERLGGKGS 240

Query: 241 VCNTYLGNNPNPMDIVRIFNFDDEICARIMRSPLVDLASSQVQPSKSNGEHEADINARDQ 300
           VCNTY+GNNPNPMDIVRIFNFDDEIC+RIMRSPL +LASSQV+ SK NGE E  IN  DQ
Sbjct: 241 VCNTYVGNNPNPMDIVRIFNFDDEICSRIMRSPLAELASSQVEDSKPNGEQEVGINLHDQ 300

Query: 301 ASEPVSMDESPLPSTDQSSDLNSESIVSPLTKVVKCSKAGDKAPPEVVKLWKENGFTSLI 360
           AS+PVSMDESPLPSTDQSSDLNSESIVSPLTKV KC+KAGDKAP EV+ LWKENGFTSLI
Sbjct: 301 ASQPVSMDESPLPSTDQSSDLNSESIVSPLTKVFKCAKAGDKAPQEVINLWKENGFTSLI 360

Query: 361 IAAPQSDTWSLVKDLLPLVANSAPFAIYHQYLQPLATCMHKLQLEKMAISLQISEPWLRE 420
           IAAPQSD WSLVKDLLPLVANSAPFAIYHQYLQPLATCMHKLQLEKMAISLQISEPWLRE
Sbjct: 361 IAAPQSDAWSLVKDLLPLVANSAPFAIYHQYLQPLATCMHKLQLEKMAISLQISEPWLRE 420

Query: 421 YQVLPSRTHPFMQMNAFGGYILSGTKICGHQ 452
           YQVLPSRTHPFMQMNAFGGYILSGTKIC HQ
Sbjct: 421 YQVLPSRTHPFMQMNAFGGYILSGTKICNHQ 451

BLAST of Lag0004462 vs. NCBI nr
Match: XP_022144389.1 (tRNA (adenine(58)-N(1))-methyltransferase non-catalytic subunit trm6 [Momordica charantia])

HSP 1 Score: 809.7 bits (2090), Expect = 1.3e-230
Identity = 417/452 (92.26%), Postives = 428/452 (94.69%), Query Frame = 0

Query: 1   MSSSILPSESPRNPRVTWEGCSVLLDINDGDRLVFARLSLASTLKIGNKSYSLKPLIGCP 60
           MSS  L SE  RNPRVTWEGCSVLLDINDGDRLVFARLS ASTLKIGNKSYSLKPLIGCP
Sbjct: 1   MSSDNLQSELHRNPRVTWEGCSVLLDINDGDRLVFARLSPASTLKIGNKSYSLKPLIGCP 60

Query: 61  FGSLFQIENGANGPHLARVMPSREEGINCQETEEMKEEQVTKDELRDNRAIVDNNKAQSL 120
           FGSLFQIEN A GPHL+RVMPSREEGINCQETE+MKEEQVTKDELRDNRAIVDNNKAQSL
Sbjct: 61  FGSLFQIENVAGGPHLSRVMPSREEGINCQETEKMKEEQVTKDELRDNRAIVDNNKAQSL 120

Query: 121 SSEDIDAMRSQGATGDEIVEALIANSATFEKKTVFSQEKYRLRKQKKYAPRVLLRRPFTR 180
           SSEDIDAMRSQGATGDEIVEALIANSATFEKKTVFSQEKYRLRKQKKYAPRVL+RRPFTR
Sbjct: 121 SSEDIDAMRSQGATGDEIVEALIANSATFEKKTVFSQEKYRLRKQKKYAPRVLVRRPFTR 180

Query: 181 SICEAYFKKYPARIGFLRVDALSLLLSFANVTANSDVLVFDMVGGLLTGAVAERLGGKGS 240
           SICEAYFKKYPARIGFLRVDALSLLLSFANVT+NSDVLV DMVGGLLTGAVAERLGGKGS
Sbjct: 181 SICEAYFKKYPARIGFLRVDALSLLLSFANVTSNSDVLVLDMVGGLLTGAVAERLGGKGS 240

Query: 241 VCNTYLGNNPNPMDIVRIFNFDDEICARIMRSPLVDLASSQVQPSKSNGEHEADINARDQ 300
           VCNTYLGNNPN MDIVRIFNFDDEICARIMRSPL+DLASSQV  SKSNGEHEA INA +Q
Sbjct: 241 VCNTYLGNNPNHMDIVRIFNFDDEICARIMRSPLIDLASSQVDSSKSNGEHEASINAEEQ 300

Query: 301 ASEPVSMDESPLPSTDQSSDLNSESIVSPLTKVVKCSKAGDKAPPEVVKLWKENGFTSLI 360
           ASEPVSMDES +PSTDQ S+LNSESIVSPLTK  KC+KAGDKAP EVV  WKENGFTSLI
Sbjct: 301 ASEPVSMDESAVPSTDQPSELNSESIVSPLTKAFKCAKAGDKAPSEVVNSWKENGFTSLI 360

Query: 361 IAAPQSDTWSLVKDLLPLVANSAPFAIYHQYLQPLATCMHKLQLEKMAISLQISEPWLRE 420
           IAAPQ D WSLVKDLLPLV+NSAPFAIYHQYLQPLATCMHKLQLE+MAISLQISEPWLRE
Sbjct: 361 IAAPQLDAWSLVKDLLPLVSNSAPFAIYHQYLQPLATCMHKLQLERMAISLQISEPWLRE 420

Query: 421 YQVLPSRTHPFMQMNAFGGYILSGTKIC-GHQ 452
           YQVLPSRTHPFMQMNAFGGYILSGTKIC GHQ
Sbjct: 421 YQVLPSRTHPFMQMNAFGGYILSGTKICGGHQ 452

BLAST of Lag0004462 vs. NCBI nr
Match: XP_011649656.1 (tRNA (adenine(58)-N(1))-methyltransferase non-catalytic subunit trm6 isoform X1 [Cucumis sativus] >KGN62671.1 hypothetical protein Csa_022108 [Cucumis sativus])

HSP 1 Score: 806.6 bits (2082), Expect = 1.1e-229
Identity = 410/452 (90.71%), Postives = 430/452 (95.13%), Query Frame = 0

Query: 1   MSSSILPSESPRNPRVTWEGCSVLLDINDGDRLVFARLSLASTLKIGNKSYSLKPLIGCP 60
           MS +IL SES RNPR+TWEGCSVLLD+NDGDRLVFARLS ASTLKIGNKS+SLKPLIGCP
Sbjct: 1   MSLTILQSESRRNPRLTWEGCSVLLDVNDGDRLVFARLSPASTLKIGNKSFSLKPLIGCP 60

Query: 61  FGSLFQIENGANGPHLARVMPSR-EEGINCQETEEMKEEQVTKDELRDNRAIVDNNKAQS 120
           FGSLFQIENG NGP LARVMPSR EEGI+CQETEEMKEEQVTKDELRDNRAIVDNNKAQS
Sbjct: 61  FGSLFQIENGGNGPELARVMPSREEEGISCQETEEMKEEQVTKDELRDNRAIVDNNKAQS 120

Query: 121 LSSEDIDAMRSQGATGDEIVEALIANSATFEKKTVFSQEKYRLRKQKKYAPRVLLRRPFT 180
           LSSEDIDAMRSQGATGDEIVEALIANSATF+KKTVFSQEKYRL+KQKKYAPRVLLRRPFT
Sbjct: 121 LSSEDIDAMRSQGATGDEIVEALIANSATFDKKTVFSQEKYRLKKQKKYAPRVLLRRPFT 180

Query: 181 RSICEAYFKKYPARIGFLRVDALSLLLSFANVTANSDVLVFDMVGGLLTGAVAERLGGKG 240
           RSICEAYFKKYPARIGFLR+DALSLLLSF NVTANSDVLV DMVGGLLTGAVAERLGGKG
Sbjct: 181 RSICEAYFKKYPARIGFLRLDALSLLLSFGNVTANSDVLVVDMVGGLLTGAVAERLGGKG 240

Query: 241 SVCNTYLGNNPNPMDIVRIFNFDDEICARIMRSPLVDLASSQVQPSKSNGEHEADINARD 300
           SVCNTY+GN PNPMDIVRIFNFDDEIC+RIMRS L DL SSQ++P+K NGE+E  IN ++
Sbjct: 241 SVCNTYVGNTPNPMDIVRIFNFDDEICSRIMRSSLADLTSSQIEPTKPNGENEDSINVQE 300

Query: 301 QASEPVSMDESPLPSTDQSSDLNSESIVSPLTKVVKCSKAGDKAPPEVVKLWKENGFTSL 360
           QAS+PVSMDESPLPSTDQSSDLNSESIVSPLTKV KC+KAGDKAPPEV+ LWKENGFTSL
Sbjct: 301 QASQPVSMDESPLPSTDQSSDLNSESIVSPLTKVFKCAKAGDKAPPEVINLWKENGFTSL 360

Query: 361 IIAAPQSDTWSLVKDLLPLVANSAPFAIYHQYLQPLATCMHKLQLEKMAISLQISEPWLR 420
           IIAAPQSD WSLVKDLLPLVANSAPFAIYHQY+QPLATCMHKLQLEKMAISLQISEPWLR
Sbjct: 361 IIAAPQSDAWSLVKDLLPLVANSAPFAIYHQYVQPLATCMHKLQLEKMAISLQISEPWLR 420

Query: 421 EYQVLPSRTHPFMQMNAFGGYILSGTKICGHQ 452
           EYQVLPSRTHPFMQMNAFGGYILSGTKIC  Q
Sbjct: 421 EYQVLPSRTHPFMQMNAFGGYILSGTKICDRQ 452

BLAST of Lag0004462 vs. NCBI nr
Match: XP_008444799.1 (PREDICTED: tRNA (adenine(58)-N(1))-methyltransferase non-catalytic subunit trm6 [Cucumis melo])

HSP 1 Score: 799.7 bits (2064), Expect = 1.3e-227
Identity = 405/450 (90.00%), Postives = 427/450 (94.89%), Query Frame = 0

Query: 2   SSSILPSESPRNPRVTWEGCSVLLDINDGDRLVFARLSLASTLKIGNKSYSLKPLIGCPF 61
           S++IL S+S RNPR+TWEGCSVLLD+NDGDRLVFARLS ASTLKIGNKS+SLKPLIGCPF
Sbjct: 3   STTILQSDSRRNPRLTWEGCSVLLDVNDGDRLVFARLSPASTLKIGNKSFSLKPLIGCPF 62

Query: 62  GSLFQIENGANGPHLARVMPSREEGINCQETEEMKEEQVTKDELRDNRAIVDNNKAQSLS 121
           GSLFQIENG NGP LARVMPSREEGINCQETEEMKEEQVTKDELRDNRA+VDNNKAQSLS
Sbjct: 63  GSLFQIENGENGPQLARVMPSREEGINCQETEEMKEEQVTKDELRDNRALVDNNKAQSLS 122

Query: 122 SEDIDAMRSQGATGDEIVEALIANSATFEKKTVFSQEKYRLRKQKKYAPRVLLRRPFTRS 181
           SEDIDAMRSQGATGDEIVEALIANSATF+KKTVFSQEKYRLRKQKKYAPRVLLRRPFTRS
Sbjct: 123 SEDIDAMRSQGATGDEIVEALIANSATFDKKTVFSQEKYRLRKQKKYAPRVLLRRPFTRS 182

Query: 182 ICEAYFKKYPARIGFLRVDALSLLLSFANVTANSDVLVFDMVGGLLTGAVAERLGGKGSV 241
           ICEAYFKKYPARIGFLR+DALSLLLSF NVTANSDVLV DMVGGLLTGAVAERLGGKGSV
Sbjct: 183 ICEAYFKKYPARIGFLRLDALSLLLSFGNVTANSDVLVVDMVGGLLTGAVAERLGGKGSV 242

Query: 242 CNTYLGNNPNPMDIVRIFNFDDEICARIMRSPLVDLASSQVQPSKSNGEHEADINARDQA 301
           CNTY+GN PNPMDIVRIFNFDDEIC+RIMRS L DL SSQ++P+K NGE++      +QA
Sbjct: 243 CNTYVGNTPNPMDIVRIFNFDDEICSRIMRSSLADLTSSQIEPTKPNGENKVS----EQA 302

Query: 302 SEPVSMDESPLPSTDQSSDLNSESIVSPLTKVVKCSKAGDKAPPEVVKLWKENGFTSLII 361
           S+PVSMDESPLPSTDQSSDLNSESIVSPLTKV KC+KAGDKAPPEV+ LWKENGFTSLII
Sbjct: 303 SQPVSMDESPLPSTDQSSDLNSESIVSPLTKVFKCAKAGDKAPPEVINLWKENGFTSLII 362

Query: 362 AAPQSDTWSLVKDLLPLVANSAPFAIYHQYLQPLATCMHKLQLEKMAISLQISEPWLREY 421
           AAPQSD W LVKDLLPLVA+SAPFAIYHQYLQPLATCMHKLQLEKMAI+LQISEPWLREY
Sbjct: 363 AAPQSDAWRLVKDLLPLVASSAPFAIYHQYLQPLATCMHKLQLEKMAINLQISEPWLREY 422

Query: 422 QVLPSRTHPFMQMNAFGGYILSGTKICGHQ 452
           QVLPSRTHPFMQMNAFGGYILSGTKIC HQ
Sbjct: 423 QVLPSRTHPFMQMNAFGGYILSGTKICDHQ 448

BLAST of Lag0004462 vs. NCBI nr
Match: XP_004152653.1 (tRNA (adenine(58)-N(1))-methyltransferase non-catalytic subunit trm6 isoform X2 [Cucumis sativus])

HSP 1 Score: 796.6 bits (2056), Expect = 1.1e-226
Identity = 408/452 (90.27%), Postives = 427/452 (94.47%), Query Frame = 0

Query: 1   MSSSILPSESPRNPRVTWEGCSVLLDINDGDRLVFARLSLASTLKIGNKSYSLKPLIGCP 60
           MS +IL SES RNPR+TWEGCSVLLD+NDGDRLVFARLS ASTLKIGNKS+SLKPLIGCP
Sbjct: 1   MSLTILQSESRRNPRLTWEGCSVLLDVNDGDRLVFARLSPASTLKIGNKSFSLKPLIGCP 60

Query: 61  FGSLFQIENGANGPHLARVMPSR-EEGINCQETEEMKEEQVTKDELRDNRAIVDNNKAQS 120
           FGSLFQIENG NGP LARVMPSR EEGI+CQETEEMKEEQVTKDELRDNRAIVDNNKAQS
Sbjct: 61  FGSLFQIENGGNGPELARVMPSREEEGISCQETEEMKEEQVTKDELRDNRAIVDNNKAQS 120

Query: 121 LSSEDIDAMRSQGATGDEIVEALIANSATFEKKTVFSQEKYRLRKQKKYAPRVLLRRPFT 180
           LSSEDIDAMRSQGATGDEIVEALIANSATF+KKTVFSQEKYRL+KQKKYAPRVLLRRPFT
Sbjct: 121 LSSEDIDAMRSQGATGDEIVEALIANSATFDKKTVFSQEKYRLKKQKKYAPRVLLRRPFT 180

Query: 181 RSICEAYFKKYPARIGFLRVDALSLLLSFANVTANSDVLVFDMVGGLLTGAVAERLGGKG 240
           RSICEAYFKKYPARIGFLR+DALSLLLSF NVTANSDVLV DMVGGLLTGAVAERLGGKG
Sbjct: 181 RSICEAYFKKYPARIGFLRLDALSLLLSFGNVTANSDVLVVDMVGGLLTGAVAERLGGKG 240

Query: 241 SVCNTYLGNNPNPMDIVRIFNFDDEICARIMRSPLVDLASSQVQPSKSNGEHEADINARD 300
           SVCNTY+GN PNPMDIVRIFNFDDEIC+RIMRS L DL SSQ++P+K NGE+E      +
Sbjct: 241 SVCNTYVGNTPNPMDIVRIFNFDDEICSRIMRSSLADLTSSQIEPTKPNGENEDS----E 300

Query: 301 QASEPVSMDESPLPSTDQSSDLNSESIVSPLTKVVKCSKAGDKAPPEVVKLWKENGFTSL 360
           QAS+PVSMDESPLPSTDQSSDLNSESIVSPLTKV KC+KAGDKAPPEV+ LWKENGFTSL
Sbjct: 301 QASQPVSMDESPLPSTDQSSDLNSESIVSPLTKVFKCAKAGDKAPPEVINLWKENGFTSL 360

Query: 361 IIAAPQSDTWSLVKDLLPLVANSAPFAIYHQYLQPLATCMHKLQLEKMAISLQISEPWLR 420
           IIAAPQSD WSLVKDLLPLVANSAPFAIYHQY+QPLATCMHKLQLEKMAISLQISEPWLR
Sbjct: 361 IIAAPQSDAWSLVKDLLPLVANSAPFAIYHQYVQPLATCMHKLQLEKMAISLQISEPWLR 420

Query: 421 EYQVLPSRTHPFMQMNAFGGYILSGTKICGHQ 452
           EYQVLPSRTHPFMQMNAFGGYILSGTKIC  Q
Sbjct: 421 EYQVLPSRTHPFMQMNAFGGYILSGTKICDRQ 448

BLAST of Lag0004462 vs. ExPASy Swiss-Prot
Match: Q54UB1 (tRNA (adenine(58)-N(1))-methyltransferase non-catalytic subunit trm6 OS=Dictyostelium discoideum OX=44689 GN=trmt6 PE=3 SV=1)

HSP 1 Score: 217.6 bits (553), Expect = 2.8e-55
Identity = 147/438 (33.56%), Postives = 240/438 (54.79%), Query Frame = 0

Query: 19  EGCSVLLDINDGDRLVFARLSLASTLKIGNKSYSLKPLIGCPFGSLFQIENGANGPHLAR 78
           EG  V+LDIN+G++    ++ L S +KIG K   +  +IG  + S FQ+ N  N      
Sbjct: 37  EGDHVILDINNGEKFSVIKVKLGSKVKIGKKQILINSIIGESYYSSFQVSNEKN------ 96

Query: 79  VMPSREEGINCQETEEMKEEQVTKDEL-RDNRAIVDNNKAQSLSSEDIDAMRSQGATGDE 138
                 E I  +E ++     V  ++   DNR +  NN AQ L+ EDI+ M+ +G   + 
Sbjct: 97  ----TLERITQKEIDDRLNNLVELNQNDADNRNLDQNNTAQKLTQEDINEMKQKGTDSNT 156

Query: 139 IVEALIANSATFEKKTVFSQEKYRLRKQKKYAPRVLLRRPFTRSICEAYFKKYPARIGFL 198
           I++ ++ NS +F+ KT FSQ KY  +K KKY+  V + +P  +S+ EAY+KK   +I  L
Sbjct: 157 IIKTIVENSESFKTKTSFSQIKYLKKKIKKYSTIVKIIKPTLKSLTEAYYKKDSRKICGL 216

Query: 199 RVDALSLLLSFANVTANSDVLVFDMVGGLLTGAVAERLGGKGSVCNTYLGNNPNPMDIVR 258
           R D+   LL+  N+ ANS VLV +   GL+TG++AER+ G+G++ + Y+G  P+ + IV 
Sbjct: 217 RFDSFGQLLTLGNIRANSQVLVVETCMGLVTGSIAERMNGQGTILSAYIGKGPS-LSIVN 276

Query: 259 IFNFDDEICARIMRSPLVDLAS--------SQVQPSKSNGEHEADINARDQASEPVSMDE 318
            F F+ ++   I    L ++ S        S++ P+ S+G ++  +  +++  E    DE
Sbjct: 277 NFGFNTDVLNTIYPFNL-NITSVLNKGEDISKLPPTASSGIYDKQVKEKEKEKE---KDE 336

Query: 319 SPLPSTDQSSDLNSESIVSPLTKVVKCSKAGDKAPPEVVKLWKENGFTSLIIAAPQSDTW 378
           +     ++ S   +++I+         ++  D +   +VKL K+ G  SL+I    S   
Sbjct: 337 N--VKDEKESGEEAKTII---------NQRNDTSNENIVKLLKDGGVWSLVIVTKYSPL- 396

Query: 379 SLVKDLLPLVANSAPFAIYHQYLQPLATCMHKLQLEKMAISLQISEPWLREYQVLPSRTH 438
           +++    P + +S  F IY Q+ QPL      L   +MA++ QISE W+RE+QVLP RTH
Sbjct: 397 NILLSCWPYLNSSGSFVIYSQFPQPLMEVHQFLHKNQMAVNQQISEIWMREHQVLPKRTH 447

Query: 439 PFMQMNAFGGYILSGTKI 448
           P M M+   G+IL GTK+
Sbjct: 457 PMMGMDGASGFILYGTKV 447

BLAST of Lag0004462 vs. ExPASy Swiss-Prot
Match: Q2T9V5 (tRNA (adenine(58)-N(1))-methyltransferase non-catalytic subunit TRM6 OS=Bos taurus OX=9913 GN=TRMT6 PE=2 SV=1)

HSP 1 Score: 189.1 bits (479), Expect = 1.1e-46
Identity = 140/454 (30.84%), Postives = 221/454 (48.68%), Query Frame = 0

Query: 27  INDGDRLVFARLSLASTLKIGNKS--------YSLKPLIGCPFGSLFQIENGANGPHLAR 86
           I DGD +V  R  +   +++  +         + L  +IG  +G+ F++ NG +      
Sbjct: 20  IRDGDFVVLKREDVFKAVQVQRRKKVTFEKQWFYLDNIIGHSYGTTFEVTNGGS------ 79

Query: 87  VMPSREEGINCQETEEMKEEQVTKDELRDNRAIVDNNKAQSLSSEDIDAMRSQGATGDEI 146
           + P +++       EE   E  TK+   DNR I+D+ K+Q L+ +DI A++ +G  G+EI
Sbjct: 80  LQPKKKK-------EEPTSE--TKEAGTDNRNIIDDGKSQKLTQDDIKALKDKGIKGEEI 139

Query: 147 VEALIANSATFEKKTVFSQEKYRLRKQKKYAPRVLLRRPFTRSICEAYFKKYPARIGFLR 206
           V+ LI NS TF  KT F+Q+KY  +K+KKY   + + +P TR +   Y+ + P +I  +R
Sbjct: 140 VQQLIENSTTFRDKTEFAQDKYIKKKKKKYEAMITVVKPSTRILSVMYYAREPGKINHMR 199

Query: 207 VDALSLLLSFANVTANSDVLVFDMVGGLLTGAVAERLGGKGSVCNTYLGNNPNPMDIVRI 266
            D L+ +L+  N+ A + ++V +   GL+ GA+ ER+GG GS+   Y G  P        
Sbjct: 200 YDTLAQMLTLGNIRAGNKMIVMETCAGLVLGAMMERMGGFGSIIQLYPGGGP-VRAATAC 259

Query: 267 FNFDDEICARIMRSPL------------VDLASSQ----VQPSKSNG----------EHE 326
           F F     + +   PL             ++ SS+        +SNG          E+E
Sbjct: 260 FGFPKSFLSGLYEFPLNKVDSLLNGTFSAEMLSSEPKDIASVEESNGTLEEKQTSEQENE 319

Query: 327 ADINARDQASEPVSMDESPLPS--TDQSSDLNSESIVSPLTKVVKCSKAGDKAPPEVVKL 386
             I    +++ P   +   + S   D      S S    + +  +  +   K   E   L
Sbjct: 320 DSIAEAPESNHPEEQERMEIVSQDPDYKEPKESGSKKDYIQEKQRRQEEQKKRHLEAAAL 379

Query: 387 WKENGFTSLIIAAPQSDTWSLVKDLLPLVANSAPFAIYHQYLQPLATCMHKLQLEKMAIS 445
             E     LI+A+    T  L+  LL  VA S PF +Y QY +PL  C  KL+     I+
Sbjct: 380 LSERNADGLIVASRFHPT-PLLLSLLDFVAPSRPFVVYCQYKEPLLECYTKLRERGGVIN 439

BLAST of Lag0004462 vs. ExPASy Swiss-Prot
Match: Q9UJA5 (tRNA (adenine(58)-N(1))-methyltransferase non-catalytic subunit TRM6 OS=Homo sapiens OX=9606 GN=TRMT6 PE=1 SV=1)

HSP 1 Score: 185.3 bits (469), Expect = 1.6e-45
Identity = 142/457 (31.07%), Postives = 221/457 (48.36%), Query Frame = 0

Query: 27  INDGDRLVFARLSLASTLKIGNKS--------YSLKPLIGCPFGSLFQIENGAN-GPHLA 86
           I DGD +V  R  +   +++  +         + L  +IG  +G+ F++ +G +  P   
Sbjct: 20  IRDGDFVVLKREDVFKAVQVQRRKKVTFEKQWFYLDNVIGHSYGTAFEVTSGGSLQPKKK 79

Query: 87  RVMPSREEGINCQETEEMKEEQVTKDELRDNRAIVDNNKAQSLSSEDIDAMRSQGATGDE 146
           R  P+ E                TK+   DNR IVD+ K+Q L+ +DI A++ +G  G+E
Sbjct: 80  REEPTAE----------------TKEAGTDNRNIVDDGKSQKLTQDDIKALKDKGIKGEE 139

Query: 147 IVEALIANSATFEKKTVFSQEKYRLRKQKKYAPRVLLRRPFTRSICEAYFKKYPARIGFL 206
           IV+ LI NS TF  KT F+Q+KY  +K+KKY   + + +P TR +   Y+ + P +I  +
Sbjct: 140 IVQQLIENSTTFRDKTEFAQDKYIKKKKKKYEAIITVVKPSTRILSIMYYAREPGKINHM 199

Query: 207 RVDALSLLLSFANVTANSDVLVFDMVGGLLTGAVAERLGGKGSVCNTYLGNNPNPMDIVR 266
           R D L+ +L+  N+ A + ++V +   GL+ GA+ ER+GG GS+   Y G  P       
Sbjct: 200 RYDTLAQMLTLGNIRAGNKMIVMETCAGLVLGAMMERMGGFGSIIQLYPGGGP-VRAATA 259

Query: 267 IFNFDDEICARIMRSPL------------VDLASSQVQPS----KSNGEHEADINARDQA 326
            F F     + +   PL              + SS+ + S    +SNG  E +  A +Q 
Sbjct: 260 CFGFPKSFLSGLYEFPLNKVDSLLHGTFSAKMLSSEPKDSALVEESNGTLE-EKQASEQE 319

Query: 327 SEPVSMDESPLPSTDQSSDLNSESIVSP--------------LTKVVKCSKAGDKAPPEV 386
           +E  SM E+P  +  +  +        P              + +  +  +   K   E 
Sbjct: 320 NED-SMAEAPESNHPEDQETMETISQDPEHKGPKERGSKKDYIQEKQRRQEEQRKRHLEA 379

Query: 387 VKLWKENGFTSLIIAAPQSDTWSLVKDLLPLVANSAPFAIYHQYLQPLATCMHKLQLEKM 445
             L  E     LI+A+    T  L+  LL  VA S PF +Y QY +PL  C  KL+    
Sbjct: 380 AALLSERNADGLIVASRFHPT-PLLLSLLDFVAPSRPFVVYCQYKEPLLECYTKLRERGG 439

BLAST of Lag0004462 vs. ExPASy Swiss-Prot
Match: Q8CE96 (tRNA (adenine(58)-N(1))-methyltransferase non-catalytic subunit TRM6 OS=Mus musculus OX=10090 GN=Trmt6 PE=1 SV=1)

HSP 1 Score: 184.9 bits (468), Expect = 2.0e-45
Identity = 144/455 (31.65%), Postives = 226/455 (49.67%), Query Frame = 0

Query: 27  INDGDRLVFARLSLASTLKIGNKS--------YSLKPLIGCPFGSLFQIENGANGPHLAR 86
           I+DGD +V  R  +   +++  +         + L   IG  +GS F + +G +      
Sbjct: 21  IHDGDFVVLKREDVFKAVQVQRRKKVTFEKQWFYLDNAIGHSYGSAFDVSSGGS------ 80

Query: 87  VMPSREEGINCQETEEMKEEQVTKDELRDNRAIVDNNKAQSLSSEDIDAMRSQGATGDEI 146
            +  R      ++ EE   E  TK+   DNR IVD+ K+Q L+ +DI A++ +G  G+EI
Sbjct: 81  -LQLR------KKLEEPASE--TKEAGTDNRNIVDDGKSQKLTQDDIKALKDKGIKGEEI 140

Query: 147 VEALIANSATFEKKTVFSQEKYRLRKQKKYAPRVLLRRPFTRSICEAYFKKYPARIGFLR 206
           V+ LI NS TF  KT F+Q+KY  +K+KKY   V + +P TR +   Y+ + P +I  +R
Sbjct: 141 VQQLIENSTTFRDKTEFAQDKYIKKKKKKYEAIVTILKPSTRILSIMYYAREPGKINHMR 200

Query: 207 VDALSLLLSFANVTANSDVLVFDMVGGLLTGAVAERLGGKGSVCNTYLGNNPNPMDIVRI 266
            D L+ +L+  N+ A + ++V +   GL+ GA+ ER+GG GS+   Y G+ P        
Sbjct: 201 YDTLAQMLTLGNIRAGNKMIVMETCSGLVLGAMMERMGGFGSIIQLYPGDGP-VRAATAC 260

Query: 267 FNFDDEICARIMRSPL------------VDLASSQVQPS----KSNGE-------HEADI 326
           F F     + +   PL             ++ SS+ + S    +SNGE        +AD 
Sbjct: 261 FGFPKSFLSGLYEFPLNKVNSLLNGTFSAEMLSSEPKDSTPVEESNGELEEKEIAEQADE 320

Query: 327 NARDQASEPVSMDESPL------PSTDQSSDLNSESIVSPLTKVVKCSKAGDKAPPEVVK 386
           +    A+E  S ++ P+      P   +  +  S+     + +  +  +   K   E   
Sbjct: 321 DNIVDAAENNSGEQRPMEIVPGDPENKEPKEKRSKR--DYIQEKQRRQEEQRKRHLEAAA 380

Query: 387 LWKENGFTSLIIAAPQSDTWSLVKDLLPLVANSAPFAIYHQYLQPLATCMHKLQLEKMAI 445
           L  E     LI+A+    T  L+  LL  VA S PF +Y QY +PL  C  KL+     I
Sbjct: 381 LLGERNADGLIVASRFHPT-PLLLSLLDFVAPSRPFVVYCQYKEPLLECYTKLRERGGVI 440

BLAST of Lag0004462 vs. ExPASy Swiss-Prot
Match: Q6BKK7 (tRNA (adenine(58)-N(1))-methyltransferase non-catalytic subunit TRM6 OS=Debaryomyces hansenii (strain ATCC 36239 / CBS 767 / BCRC 21394 / JCM 1990 / NBRC 0083 / IGC 2968) OX=284592 GN=TRM6 PE=3 SV=2)

HSP 1 Score: 141.4 bits (355), Expect = 2.6e-32
Identity = 110/406 (27.09%), Postives = 205/406 (50.49%), Query Frame = 0

Query: 50  SYSLKPLIGCPFGSLFQI-ENGANGPHLARVMPSREEGINCQETEEMKEEQVTK---DEL 109
           ++++  ++G PFG  F+I E     P  +  M + E  ++  E EE+ ++ +TK   +  
Sbjct: 63  TFAVDGILGYPFGQTFEILEELKVKPIKSMTMQADETSVD-NENEELTKDDLTKMLSNSS 122

Query: 110 RDNRAIVD-NNKAQSLSSEDIDAMRSQGAT---GDEIVEALIANSATFEKKTVFSQEKYR 169
            +N+ I++  +K Q L+SED+D ++  GAT   G  I+E +IA    F+KKT+FSQ+KY 
Sbjct: 123 DNNQNIINIGSKIQKLTSEDVDKLKESGATSDIGQRIIEQMIAGHEGFDKKTLFSQQKYL 182

Query: 170 LRKQKKYAPRVLLRRPFTRSICEAYFKKYPARIGFLRVDALSLLLSFANVTANSDVLVFD 229
            RKQ+K+  R  +    +  + + Y +K   R+  +  + L LLL++ANV      L+ D
Sbjct: 183 KRKQQKFLRRFTVEYLGSSQLLQYYIEKDTQRVLDMSEETLGLLLNYANVRPGGKYLLID 242

Query: 230 MVGGLLTGAVAERLGGKGSVCNTYLGNNPNPMDIVRIFNFDDEICARIMRSPLVDLASSQ 289
             GG++  A+ ER+ G+G++ + +   +PN +  +R  ++ +E+  R+++S  ++     
Sbjct: 243 ETGGIILYAMMERMNGQGTIVSAHDNEHPNHI-ALRYSDYPEEMQNRMVKS--INWLQF- 302

Query: 290 VQPSKSNGEHEADINARDQASEPVSMDESPLPSTDQSSDLNSESIVSPLTKVVKCSKAGD 349
           ++P     E E    A ++  E +   +       +    N  S++              
Sbjct: 303 LEPENEKVEFE---TATEEEIEEMKHAKRAQYYRREQRAKNINSVI-------------- 362

Query: 350 KAPPEVVKLWKENGFTSLIIAAPQSDTWSLVKDLLPLVANSAPFAIYHQYLQPLATCMHK 409
               ++V     +GF    I+    +  +L+ +++P +  S P  IY Q+ + L    H 
Sbjct: 363 ----DMVMEGNFDGF----ISVSTLNMPTLLPEIIPTIGGSRPVVIYSQFKEALLETQHH 422

Query: 410 LQLEKMAISLQISEPWLREYQVLPSRTHPFMQMNAFGGYILSGTKI 448
           +  +K  ++  I E  +R +Q +P R HP M M  FGGYIL GT++
Sbjct: 423 MSTDKRVLAPSIMETRVRPHQTIPGRMHPVMCMRGFGGYILWGTRV 438

BLAST of Lag0004462 vs. ExPASy TrEMBL
Match: A0A6J1CS60 (tRNA (adenine(58)-N(1))-methyltransferase non-catalytic subunit TRM6 OS=Momordica charantia OX=3673 GN=LOC111014079 PE=3 SV=1)

HSP 1 Score: 809.7 bits (2090), Expect = 6.2e-231
Identity = 417/452 (92.26%), Postives = 428/452 (94.69%), Query Frame = 0

Query: 1   MSSSILPSESPRNPRVTWEGCSVLLDINDGDRLVFARLSLASTLKIGNKSYSLKPLIGCP 60
           MSS  L SE  RNPRVTWEGCSVLLDINDGDRLVFARLS ASTLKIGNKSYSLKPLIGCP
Sbjct: 1   MSSDNLQSELHRNPRVTWEGCSVLLDINDGDRLVFARLSPASTLKIGNKSYSLKPLIGCP 60

Query: 61  FGSLFQIENGANGPHLARVMPSREEGINCQETEEMKEEQVTKDELRDNRAIVDNNKAQSL 120
           FGSLFQIEN A GPHL+RVMPSREEGINCQETE+MKEEQVTKDELRDNRAIVDNNKAQSL
Sbjct: 61  FGSLFQIENVAGGPHLSRVMPSREEGINCQETEKMKEEQVTKDELRDNRAIVDNNKAQSL 120

Query: 121 SSEDIDAMRSQGATGDEIVEALIANSATFEKKTVFSQEKYRLRKQKKYAPRVLLRRPFTR 180
           SSEDIDAMRSQGATGDEIVEALIANSATFEKKTVFSQEKYRLRKQKKYAPRVL+RRPFTR
Sbjct: 121 SSEDIDAMRSQGATGDEIVEALIANSATFEKKTVFSQEKYRLRKQKKYAPRVLVRRPFTR 180

Query: 181 SICEAYFKKYPARIGFLRVDALSLLLSFANVTANSDVLVFDMVGGLLTGAVAERLGGKGS 240
           SICEAYFKKYPARIGFLRVDALSLLLSFANVT+NSDVLV DMVGGLLTGAVAERLGGKGS
Sbjct: 181 SICEAYFKKYPARIGFLRVDALSLLLSFANVTSNSDVLVLDMVGGLLTGAVAERLGGKGS 240

Query: 241 VCNTYLGNNPNPMDIVRIFNFDDEICARIMRSPLVDLASSQVQPSKSNGEHEADINARDQ 300
           VCNTYLGNNPN MDIVRIFNFDDEICARIMRSPL+DLASSQV  SKSNGEHEA INA +Q
Sbjct: 241 VCNTYLGNNPNHMDIVRIFNFDDEICARIMRSPLIDLASSQVDSSKSNGEHEASINAEEQ 300

Query: 301 ASEPVSMDESPLPSTDQSSDLNSESIVSPLTKVVKCSKAGDKAPPEVVKLWKENGFTSLI 360
           ASEPVSMDES +PSTDQ S+LNSESIVSPLTK  KC+KAGDKAP EVV  WKENGFTSLI
Sbjct: 301 ASEPVSMDESAVPSTDQPSELNSESIVSPLTKAFKCAKAGDKAPSEVVNSWKENGFTSLI 360

Query: 361 IAAPQSDTWSLVKDLLPLVANSAPFAIYHQYLQPLATCMHKLQLEKMAISLQISEPWLRE 420
           IAAPQ D WSLVKDLLPLV+NSAPFAIYHQYLQPLATCMHKLQLE+MAISLQISEPWLRE
Sbjct: 361 IAAPQLDAWSLVKDLLPLVSNSAPFAIYHQYLQPLATCMHKLQLERMAISLQISEPWLRE 420

Query: 421 YQVLPSRTHPFMQMNAFGGYILSGTKIC-GHQ 452
           YQVLPSRTHPFMQMNAFGGYILSGTKIC GHQ
Sbjct: 421 YQVLPSRTHPFMQMNAFGGYILSGTKICGGHQ 452

BLAST of Lag0004462 vs. ExPASy TrEMBL
Match: A0A0A0LRN2 (tRNA (adenine(58)-N(1))-methyltransferase non-catalytic subunit TRM6 OS=Cucumis sativus OX=3659 GN=Csa_2G367740 PE=3 SV=1)

HSP 1 Score: 806.6 bits (2082), Expect = 5.3e-230
Identity = 410/452 (90.71%), Postives = 430/452 (95.13%), Query Frame = 0

Query: 1   MSSSILPSESPRNPRVTWEGCSVLLDINDGDRLVFARLSLASTLKIGNKSYSLKPLIGCP 60
           MS +IL SES RNPR+TWEGCSVLLD+NDGDRLVFARLS ASTLKIGNKS+SLKPLIGCP
Sbjct: 1   MSLTILQSESRRNPRLTWEGCSVLLDVNDGDRLVFARLSPASTLKIGNKSFSLKPLIGCP 60

Query: 61  FGSLFQIENGANGPHLARVMPSR-EEGINCQETEEMKEEQVTKDELRDNRAIVDNNKAQS 120
           FGSLFQIENG NGP LARVMPSR EEGI+CQETEEMKEEQVTKDELRDNRAIVDNNKAQS
Sbjct: 61  FGSLFQIENGGNGPELARVMPSREEEGISCQETEEMKEEQVTKDELRDNRAIVDNNKAQS 120

Query: 121 LSSEDIDAMRSQGATGDEIVEALIANSATFEKKTVFSQEKYRLRKQKKYAPRVLLRRPFT 180
           LSSEDIDAMRSQGATGDEIVEALIANSATF+KKTVFSQEKYRL+KQKKYAPRVLLRRPFT
Sbjct: 121 LSSEDIDAMRSQGATGDEIVEALIANSATFDKKTVFSQEKYRLKKQKKYAPRVLLRRPFT 180

Query: 181 RSICEAYFKKYPARIGFLRVDALSLLLSFANVTANSDVLVFDMVGGLLTGAVAERLGGKG 240
           RSICEAYFKKYPARIGFLR+DALSLLLSF NVTANSDVLV DMVGGLLTGAVAERLGGKG
Sbjct: 181 RSICEAYFKKYPARIGFLRLDALSLLLSFGNVTANSDVLVVDMVGGLLTGAVAERLGGKG 240

Query: 241 SVCNTYLGNNPNPMDIVRIFNFDDEICARIMRSPLVDLASSQVQPSKSNGEHEADINARD 300
           SVCNTY+GN PNPMDIVRIFNFDDEIC+RIMRS L DL SSQ++P+K NGE+E  IN ++
Sbjct: 241 SVCNTYVGNTPNPMDIVRIFNFDDEICSRIMRSSLADLTSSQIEPTKPNGENEDSINVQE 300

Query: 301 QASEPVSMDESPLPSTDQSSDLNSESIVSPLTKVVKCSKAGDKAPPEVVKLWKENGFTSL 360
           QAS+PVSMDESPLPSTDQSSDLNSESIVSPLTKV KC+KAGDKAPPEV+ LWKENGFTSL
Sbjct: 301 QASQPVSMDESPLPSTDQSSDLNSESIVSPLTKVFKCAKAGDKAPPEVINLWKENGFTSL 360

Query: 361 IIAAPQSDTWSLVKDLLPLVANSAPFAIYHQYLQPLATCMHKLQLEKMAISLQISEPWLR 420
           IIAAPQSD WSLVKDLLPLVANSAPFAIYHQY+QPLATCMHKLQLEKMAISLQISEPWLR
Sbjct: 361 IIAAPQSDAWSLVKDLLPLVANSAPFAIYHQYVQPLATCMHKLQLEKMAISLQISEPWLR 420

Query: 421 EYQVLPSRTHPFMQMNAFGGYILSGTKICGHQ 452
           EYQVLPSRTHPFMQMNAFGGYILSGTKIC  Q
Sbjct: 421 EYQVLPSRTHPFMQMNAFGGYILSGTKICDRQ 452

BLAST of Lag0004462 vs. ExPASy TrEMBL
Match: A0A1S3BB68 (tRNA (adenine(58)-N(1))-methyltransferase non-catalytic subunit TRM6 OS=Cucumis melo OX=3656 GN=LOC103488037 PE=3 SV=1)

HSP 1 Score: 799.7 bits (2064), Expect = 6.4e-228
Identity = 405/450 (90.00%), Postives = 427/450 (94.89%), Query Frame = 0

Query: 2   SSSILPSESPRNPRVTWEGCSVLLDINDGDRLVFARLSLASTLKIGNKSYSLKPLIGCPF 61
           S++IL S+S RNPR+TWEGCSVLLD+NDGDRLVFARLS ASTLKIGNKS+SLKPLIGCPF
Sbjct: 3   STTILQSDSRRNPRLTWEGCSVLLDVNDGDRLVFARLSPASTLKIGNKSFSLKPLIGCPF 62

Query: 62  GSLFQIENGANGPHLARVMPSREEGINCQETEEMKEEQVTKDELRDNRAIVDNNKAQSLS 121
           GSLFQIENG NGP LARVMPSREEGINCQETEEMKEEQVTKDELRDNRA+VDNNKAQSLS
Sbjct: 63  GSLFQIENGENGPQLARVMPSREEGINCQETEEMKEEQVTKDELRDNRALVDNNKAQSLS 122

Query: 122 SEDIDAMRSQGATGDEIVEALIANSATFEKKTVFSQEKYRLRKQKKYAPRVLLRRPFTRS 181
           SEDIDAMRSQGATGDEIVEALIANSATF+KKTVFSQEKYRLRKQKKYAPRVLLRRPFTRS
Sbjct: 123 SEDIDAMRSQGATGDEIVEALIANSATFDKKTVFSQEKYRLRKQKKYAPRVLLRRPFTRS 182

Query: 182 ICEAYFKKYPARIGFLRVDALSLLLSFANVTANSDVLVFDMVGGLLTGAVAERLGGKGSV 241
           ICEAYFKKYPARIGFLR+DALSLLLSF NVTANSDVLV DMVGGLLTGAVAERLGGKGSV
Sbjct: 183 ICEAYFKKYPARIGFLRLDALSLLLSFGNVTANSDVLVVDMVGGLLTGAVAERLGGKGSV 242

Query: 242 CNTYLGNNPNPMDIVRIFNFDDEICARIMRSPLVDLASSQVQPSKSNGEHEADINARDQA 301
           CNTY+GN PNPMDIVRIFNFDDEIC+RIMRS L DL SSQ++P+K NGE++      +QA
Sbjct: 243 CNTYVGNTPNPMDIVRIFNFDDEICSRIMRSSLADLTSSQIEPTKPNGENKVS----EQA 302

Query: 302 SEPVSMDESPLPSTDQSSDLNSESIVSPLTKVVKCSKAGDKAPPEVVKLWKENGFTSLII 361
           S+PVSMDESPLPSTDQSSDLNSESIVSPLTKV KC+KAGDKAPPEV+ LWKENGFTSLII
Sbjct: 303 SQPVSMDESPLPSTDQSSDLNSESIVSPLTKVFKCAKAGDKAPPEVINLWKENGFTSLII 362

Query: 362 AAPQSDTWSLVKDLLPLVANSAPFAIYHQYLQPLATCMHKLQLEKMAISLQISEPWLREY 421
           AAPQSD W LVKDLLPLVA+SAPFAIYHQYLQPLATCMHKLQLEKMAI+LQISEPWLREY
Sbjct: 363 AAPQSDAWRLVKDLLPLVASSAPFAIYHQYLQPLATCMHKLQLEKMAINLQISEPWLREY 422

Query: 422 QVLPSRTHPFMQMNAFGGYILSGTKICGHQ 452
           QVLPSRTHPFMQMNAFGGYILSGTKIC HQ
Sbjct: 423 QVLPSRTHPFMQMNAFGGYILSGTKICDHQ 448

BLAST of Lag0004462 vs. ExPASy TrEMBL
Match: A0A6J1GI99 (tRNA (adenine(58)-N(1))-methyltransferase non-catalytic subunit TRM6 OS=Cucurbita moschata OX=3662 GN=LOC111454455 PE=3 SV=1)

HSP 1 Score: 782.7 bits (2020), Expect = 8.2e-223
Identity = 406/448 (90.62%), Postives = 420/448 (93.75%), Query Frame = 0

Query: 1   MSSSILPSESPRNPRVTWEGCSVLLDINDGDRLVFARLSLASTLKIGNKSYSLKPLIGCP 60
           MSSS L SESPRNPRV WEGCSVLLDINDGDRLVFARLS ASTLKIGNKSYSLKPLIG P
Sbjct: 1   MSSSNLQSESPRNPRVAWEGCSVLLDINDGDRLVFARLSPASTLKIGNKSYSLKPLIGRP 60

Query: 61  FGSLFQIENGANGPHLARVMPSREEGINCQETEEMKEEQVTKDELRDNRAIVDNNKAQSL 120
           FGSLFQIE+G NG HLA V PSREE     ETEEMKEEQV KDELRDNRA+VDNNKAQSL
Sbjct: 61  FGSLFQIESGDNGQHLACVTPSREE-----ETEEMKEEQVVKDELRDNRALVDNNKAQSL 120

Query: 121 SSEDIDAMRSQGATGDEIVEALIANSATFEKKTVFSQEKYRLRKQKKYAPRVLLRRPFTR 180
           SSEDIDAMRSQGATGDEIVEALIANSATFEKKTVFSQEKYRL+KQKKYAPRVLLRRPFTR
Sbjct: 121 SSEDIDAMRSQGATGDEIVEALIANSATFEKKTVFSQEKYRLKKQKKYAPRVLLRRPFTR 180

Query: 181 SICEAYFKKYPARIGFLRVDALSLLLSFANVTANSDVLVFDMVGGLLTGAVAERLGGKGS 240
           SICEAYFKK+PARIGFLRVDALSLLLSFANVT+NSDVLVFDMVGGL+TGAVAERLGGKGS
Sbjct: 181 SICEAYFKKFPARIGFLRVDALSLLLSFANVTSNSDVLVFDMVGGLITGAVAERLGGKGS 240

Query: 241 VCNTYLGNNPNPMDIVRIFNFDDEICARIMRSPLVDLASSQVQPSKSNGEHEADINARDQ 300
           VCNTYLG+NPNPM+IVR+FNFDDEICARIMRSPL DL SSQV    SNGEHE  I A+D+
Sbjct: 241 VCNTYLGSNPNPMEIVRMFNFDDEICARIMRSPLTDLTSSQV----SNGEHEVTIKAQDE 300

Query: 301 ASEPVSMDESPLPSTDQSSDLNSESIVSPLTKVVKCSKAGDKAPPEVVKLWKENGFTSLI 360
           ASEPV+MDE PLPSTDQSSDLNSESIVSPLTKV+KC+KAGDKAPPEVVK WKENGFTSLI
Sbjct: 301 ASEPVNMDEGPLPSTDQSSDLNSESIVSPLTKVLKCAKAGDKAPPEVVKSWKENGFTSLI 360

Query: 361 IAAPQSDTWSLVKDLLPLVANSAPFAIYHQYLQPLATCMHKLQLEKMAISLQISEPWLRE 420
           IAAPQSD W LVKDLLPLVANSAPFAIYHQYLQPLATCMHKLQLEKMAISLQISEPWLRE
Sbjct: 361 IAAPQSDAWGLVKDLLPLVANSAPFAIYHQYLQPLATCMHKLQLEKMAISLQISEPWLRE 420

Query: 421 YQVLPSRTHPFMQMNAFGGYILSGTKIC 449
           YQVLPSRTHPFMQMNAFGGYILSGTKIC
Sbjct: 421 YQVLPSRTHPFMQMNAFGGYILSGTKIC 439

BLAST of Lag0004462 vs. ExPASy TrEMBL
Match: A0A6J1KL25 (tRNA (adenine(58)-N(1))-methyltransferase non-catalytic subunit TRM6 OS=Cucurbita maxima OX=3661 GN=LOC111496211 PE=3 SV=1)

HSP 1 Score: 780.8 bits (2015), Expect = 3.1e-222
Identity = 405/448 (90.40%), Postives = 420/448 (93.75%), Query Frame = 0

Query: 1   MSSSILPSESPRNPRVTWEGCSVLLDINDGDRLVFARLSLASTLKIGNKSYSLKPLIGCP 60
           MSSS LPSESPRNPRV WEGCSVLLDINDGDRLVFARLS ASTLKIGNKSYSLKPLIG P
Sbjct: 1   MSSSNLPSESPRNPRVAWEGCSVLLDINDGDRLVFARLSPASTLKIGNKSYSLKPLIGRP 60

Query: 61  FGSLFQIENGANGPHLARVMPSREEGINCQETEEMKEEQVTKDELRDNRAIVDNNKAQSL 120
           FGSLFQIE+G NG HLA V PSREE     ETEEMKEEQV KDELRDNRA+VDNNKAQSL
Sbjct: 61  FGSLFQIESGDNGQHLACVTPSREE-----ETEEMKEEQVVKDELRDNRALVDNNKAQSL 120

Query: 121 SSEDIDAMRSQGATGDEIVEALIANSATFEKKTVFSQEKYRLRKQKKYAPRVLLRRPFTR 180
           SSEDIDAMRSQGATGDEIVEALIANSATFEKKTVFSQEKYRL+KQKKYAPRVLLRRPFTR
Sbjct: 121 SSEDIDAMRSQGATGDEIVEALIANSATFEKKTVFSQEKYRLKKQKKYAPRVLLRRPFTR 180

Query: 181 SICEAYFKKYPARIGFLRVDALSLLLSFANVTANSDVLVFDMVGGLLTGAVAERLGGKGS 240
           SICEAYFKK+PARIGFLRVDALSLLLSFANVT+NSDVLVFDMVGGL+TGAVAERLGGKGS
Sbjct: 181 SICEAYFKKFPARIGFLRVDALSLLLSFANVTSNSDVLVFDMVGGLITGAVAERLGGKGS 240

Query: 241 VCNTYLGNNPNPMDIVRIFNFDDEICARIMRSPLVDLASSQVQPSKSNGEHEADINARDQ 300
           VCN YLG+NPNPM+IVR+FNFDDEICARIMRSPL DLASSQV    SNGEHE +I A+D+
Sbjct: 241 VCNAYLGSNPNPMEIVRMFNFDDEICARIMRSPLTDLASSQV----SNGEHEVNIKAQDE 300

Query: 301 ASEPVSMDESPLPSTDQSSDLNSESIVSPLTKVVKCSKAGDKAPPEVVKLWKENGFTSLI 360
           ASEPV+MDE P PSTDQSS LNSESIVSPLTKV+KC+KAGDKAPPEVVK WKENGFTSLI
Sbjct: 301 ASEPVNMDEGPPPSTDQSSGLNSESIVSPLTKVLKCAKAGDKAPPEVVKSWKENGFTSLI 360

Query: 361 IAAPQSDTWSLVKDLLPLVANSAPFAIYHQYLQPLATCMHKLQLEKMAISLQISEPWLRE 420
           IAAPQSD W LVKDLLPLVANSAPFAIYHQYLQPLATCMHKLQLEKMAISLQISEPWLRE
Sbjct: 361 IAAPQSDAWGLVKDLLPLVANSAPFAIYHQYLQPLATCMHKLQLEKMAISLQISEPWLRE 420

Query: 421 YQVLPSRTHPFMQMNAFGGYILSGTKIC 449
           YQVLPSRTHPFMQMNAFGGYILSGTKIC
Sbjct: 421 YQVLPSRTHPFMQMNAFGGYILSGTKIC 439

BLAST of Lag0004462 vs. TAIR 10
Match: AT2G45730.1 (eukaryotic initiation factor 3 gamma subunit family protein )

HSP 1 Score: 523.9 bits (1348), Expect = 1.3e-148
Identity = 267/435 (61.38%), Postives = 337/435 (77.47%), Query Frame = 0

Query: 13  NPRVTWEGCSVLLDINDGDRLVFARLSLASTLKIGNKSYSLKPLIGCPFGSLFQIENGAN 72
           NPR   EGCSVLLDINDGDRLVFARLS  + LKIGNK+YSLKPLIG PFGSLFQ+E G +
Sbjct: 20  NPRFACEGCSVLLDINDGDRLVFARLSSGAILKIGNKNYSLKPLIGAPFGSLFQVETGED 79

Query: 73  GPHLARVMPSREEGINCQETEEMKEEQVTKDELRDNRAIVDNNKAQSLSSEDIDAMRSQG 132
           G  L+R++P ++E                 D+ RDNR IVDNN+AQ+L+ E+I+AMR +G
Sbjct: 80  GSFLSRILPIKQE----------SNSNNVMDDSRDNREIVDNNEAQNLTGEEIEAMRREG 139

Query: 133 ATGDEIVEALIANSATFEKKTVFSQEKYRLRKQKKYAPRVLLRRPFTRSICEAYFKKYPA 192
           A GDEI+EALIANS TF+KK   SQEKY+L+KQKKYAP++LLRRPF RSICEAYFKKYPA
Sbjct: 140 AKGDEIIEALIANSKTFDKKFQLSQEKYKLKKQKKYAPKLLLRRPFARSICEAYFKKYPA 199

Query: 193 RIGFLRVDALSLLLSFANVTANSDVLVFDMVGGLLTGAVAERLGGKGSVCNTYLGNNPNP 252
           RIGFLRVDALSLLL+ ANVTA SDVLV DMVGGL+TGAVAERLGG G VCNTY G++P P
Sbjct: 200 RIGFLRVDALSLLLTMANVTAYSDVLVVDMVGGLVTGAVAERLGGTGYVCNTYKGSSPYP 259

Query: 253 MDIVRIFNFDDEICARIMRSPLVDLASSQVQPSKSNGEHEADINARDQASEPVSMDESPL 312
           +++VR+FNF D++  RI++S + +L+S++   ++ N + E    A +  +E  + D   +
Sbjct: 260 VEMVRMFNFSDKVLKRIVQSSINELSSAKTASTEGNNQQEGVCKAENNINETATSDVM-V 319

Query: 313 PSTDQSSDLNSESIVSPLTKVVKCSKAGDKAPPEVVKLWKENGFTSLIIAAPQSDTWSLV 372
                +++     IV P +K++K +KAG KA  E +++W+ENGF+SLI+AA   D W + 
Sbjct: 320 EEISVTTEAGVVDIVVPESKIIKAAKAGAKASKETIEMWQENGFSSLIMAAQDQDPWIVA 379

Query: 373 KDLLPLVANSAPFAIYHQYLQPLATCMHKLQLEKMAISLQISEPWLREYQVLPSRTHPFM 432
           KD+LPL++ SAPFAIYHQYLQPLATCMHKLQ  KMAI+LQI+EPWLREYQVLPSR+HP M
Sbjct: 380 KDVLPLLSYSAPFAIYHQYLQPLATCMHKLQQGKMAINLQITEPWLREYQVLPSRSHPHM 439

Query: 433 QMNAFGGYILSGTKI 448
           QM++FGGY+LSG +I
Sbjct: 440 QMSSFGGYVLSGIRI 443

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
XP_038884150.16.0e-23693.13tRNA (adenine(58)-N(1))-methyltransferase non-catalytic subunit trm6 [Benincasa ... [more]
XP_022144389.11.3e-23092.26tRNA (adenine(58)-N(1))-methyltransferase non-catalytic subunit trm6 [Momordica ... [more]
XP_011649656.11.1e-22990.71tRNA (adenine(58)-N(1))-methyltransferase non-catalytic subunit trm6 isoform X1 ... [more]
XP_008444799.11.3e-22790.00PREDICTED: tRNA (adenine(58)-N(1))-methyltransferase non-catalytic subunit trm6 ... [more]
XP_004152653.11.1e-22690.27tRNA (adenine(58)-N(1))-methyltransferase non-catalytic subunit trm6 isoform X2 ... [more]
Match NameE-valueIdentityDescription
Q54UB12.8e-5533.56tRNA (adenine(58)-N(1))-methyltransferase non-catalytic subunit trm6 OS=Dictyost... [more]
Q2T9V51.1e-4630.84tRNA (adenine(58)-N(1))-methyltransferase non-catalytic subunit TRM6 OS=Bos taur... [more]
Q9UJA51.6e-4531.07tRNA (adenine(58)-N(1))-methyltransferase non-catalytic subunit TRM6 OS=Homo sap... [more]
Q8CE962.0e-4531.65tRNA (adenine(58)-N(1))-methyltransferase non-catalytic subunit TRM6 OS=Mus musc... [more]
Q6BKK72.6e-3227.09tRNA (adenine(58)-N(1))-methyltransferase non-catalytic subunit TRM6 OS=Debaryom... [more]
Match NameE-valueIdentityDescription
A0A6J1CS606.2e-23192.26tRNA (adenine(58)-N(1))-methyltransferase non-catalytic subunit TRM6 OS=Momordic... [more]
A0A0A0LRN25.3e-23090.71tRNA (adenine(58)-N(1))-methyltransferase non-catalytic subunit TRM6 OS=Cucumis ... [more]
A0A1S3BB686.4e-22890.00tRNA (adenine(58)-N(1))-methyltransferase non-catalytic subunit TRM6 OS=Cucumis ... [more]
A0A6J1GI998.2e-22390.63tRNA (adenine(58)-N(1))-methyltransferase non-catalytic subunit TRM6 OS=Cucurbit... [more]
A0A6J1KL253.1e-22290.40tRNA (adenine(58)-N(1))-methyltransferase non-catalytic subunit TRM6 OS=Cucurbit... [more]
Match NameE-valueIdentityDescription
AT2G45730.11.3e-14861.38eukaryotic initiation factor 3 gamma subunit family protein [more]
InterPro
Analysis Name: InterPro Annotations of Sponge gourd (AG-4) v1
Date Performed: 2022-08-01
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR017423tRNA (adenine(58)-N(1))-methyltransferase non-catalytic subunit TRM6PFAMPF04189Gcd10pcoord: 22..272
e-value: 2.1E-58
score: 197.9
IPR017423tRNA (adenine(58)-N(1))-methyltransferase non-catalytic subunit TRM6PIRSFPIRSF038170TRM6coord: 9..451
e-value: 1.1E-127
score: 424.3
IPR017423tRNA (adenine(58)-N(1))-methyltransferase non-catalytic subunit TRM6PANTHERPTHR12945TRANSLATION INITIATION FACTOR EIF3-RELATEDcoord: 12..449
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 300..323
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 307..323

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Lag0004462.1Lag0004462.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0030488 tRNA methylation
cellular_component GO:0005634 nucleus
cellular_component GO:0031515 tRNA (m1A) methyltransferase complex
molecular_function GO:0008168 methyltransferase activity