Lag0004425 (gene) Sponge gourd (AG‐4) v1

Overview


Name	Lag0004425
Type	gene
Organism	Luffa acutangula (Sponge gourd (AG‐4) v1)
Description	thioredoxin H1-like
Location	chr6: 3719442 .. 3720442 (-)
RNA-Seq Expression	Lag0004425
Synteny	Lag0004425

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: polypeptideexonCDS

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGGCTGAAGAAGGGCAAGTGATTGCATGTCATAAGAAAGCCGAATGGGATGCTCAATTAACAAAAGCGAAGGAATCAGGAAAACTGGTATCGTTTTCTTGATTTTTCGGAGTCTTCATTTCTACATTTCAATTGATTCCGATGGAATTTTGGATTCTCTGGATAGATATGTTGAAGCATTTTCATGATTCCTTTCGCCTTTTCCTGTTCTGTTAGGACATTACATTGTTCGATGTTTTGGTAAAAGTCGCGATGCATTCGTTAGGGTTTCTGATCTCTTTTATAGGCTAGTTATATTCGATTTATCTGGATTGAATGATCGTGTTGAAATTGATGCTTTCGATGTTTCCTTTTTGACGTCTTCTGGAATCTTTGATTTGTATTCTTCAATCCTTCTCTGTTACTCTTTTCGTTCTCTTTTTTGTTTTTTTTTTTTAAACTTGATTATGCTATTTTGCATTGACCGAACTTGAGTGATTCTGTGTTACATGGTAGTATTCCAGTTTGTGTCCATTTTTTGTTCCTGACGTTTTTTGTTTGGTAGATTGTGGTGGATTTTACTGCTTCCTGGTGCGGGCCATGCCGTGCAATTGCTCCATATTTCTCAGAATTGGCTAAGAATCATTCCAGTGTCATTTTCCTGAAAGTGGACGTTGATGAATTGAATGTAAAGATATCTAAGCTTTTTTTTAATAACTCTTTCCTTTTTATTATATCATATGTCAACACTTCTGTTCCTTCAGGATATTGCTGAATACAGTAGCATGCATGCACTGGAACTGTTGTCTTTTGACTCGATCCCTGTTTTCCCCCCCTCAAACAATGGCTTCTGTTTTTCTCTGCAGACTATTGCTAAAGAGTGGGAGATTGATGCAATGCCCACGTTTGTTTTCCTGAAAGGAGGGGAAAAAATTCACAAGATTGTTGGTGCTGATAAAGTGGCGCTGTTGAAGAAAATAGAAGAGCTTAAAGCTTCAGCAGCTGCTACTTCTACTGCTTAG

mRNA sequence

ATGGCTGAAGAAGGGCAAGTGATTGCATGTCATAAGAAAGCCGAATGGGATGCTCAATTAACAAAAGCGAAGGAATCAGGAAAACTGATTGTGGTGGATTTTACTGCTTCCTGGTGCGGGCCATGCCGTGCAATTGCTCCATATTTCTCAGAATTGGCTAAGAATCATTCCAGTGTCATTTTCCTGAAAGTGGACGTTGATGAATTGAATGATATTGCTGAATACAGTAGCATGCATGCACTGGAACTGTTGTCTTTTGACTCGATCCCTGTTTTCCCCCCCTCAAACAATGGCTTCTGTTTTTCTCTGCAGACTATTGCTAAAGAGTGGGAGATTGATGCAATGCCCACGTTTGTTTTCCTGAAAGGAGGGGAAAAAATTCACAAGATTGTTGGTGCTGATAAAGTGGCGCTGTTGAAGAAAATAGAAGAGCTTAAAGCTTCAGCAGCTGCTACTTCTACTGCTTAG

Coding sequence (CDS)

ATGGCTGAAGAAGGGCAAGTGATTGCATGTCATAAGAAAGCCGAATGGGATGCTCAATTAACAAAAGCGAAGGAATCAGGAAAACTGATTGTGGTGGATTTTACTGCTTCCTGGTGCGGGCCATGCCGTGCAATTGCTCCATATTTCTCAGAATTGGCTAAGAATCATTCCAGTGTCATTTTCCTGAAAGTGGACGTTGATGAATTGAATGATATTGCTGAATACAGTAGCATGCATGCACTGGAACTGTTGTCTTTTGACTCGATCCCTGTTTTCCCCCCCTCAAACAATGGCTTCTGTTTTTCTCTGCAGACTATTGCTAAAGAGTGGGAGATTGATGCAATGCCCACGTTTGTTTTCCTGAAAGGAGGGGAAAAAATTCACAAGATTGTTGGTGCTGATAAAGTGGCGCTGTTGAAGAAAATAGAAGAGCTTAAAGCTTCAGCAGCTGCTACTTCTACTGCTTAG

Protein sequence

MAEEGQVIACHKKAEWDAQLTKAKESGKLIVVDFTASWCGPCRAIAPYFSELAKNHSSVIFLKVDVDELNDIAEYSSMHALELLSFDSIPVFPPSNNGFCFSLQTIAKEWEIDAMPTFVFLKGGEKIHKIVGADKVALLKKIEELKASAAATSTA

Homology

[Close]

BLAST of Lag0004425 vs. NCBI nr
Match: XP_023537338.1 (thioredoxin H-type-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 194.9 bits (494), Expect = 5.2e-46
Identity = 105/156 (67.31%), Postives = 111/156 (71.15%), Query Frame = 0


Query: 1   MAEEGQVIACHKKAEWDAQLTKAKESGKLIVVDFTASWCGPCRAIAPYFSELAKNHSSVI 60
           MAEEGQVIACHKKAEWDA L KAKESGKLIVVDFTASWCGPCRAIAPYFSELAKNH  V+
Sbjct: 1   MAEEGQVIACHKKAEWDAHLAKAKESGKLIVVDFTASWCGPCRAIAPYFSELAKNHPGVV 60

Query: 61  FLKVDVDELNDIAEYSSMHALELLSFDSIPVFPPSNNGFCFSLQTIAKEWEIDAMPTFVF 120
           FLKVDVDELN                                  T+A EWEI+AMPTFVF
Sbjct: 61  FLKVDVDELN----------------------------------TVATEWEINAMPTFVF 120

Query: 121 LKGGEKIHKIVGADKVALLKKIEELKA-SAAATSTA 156
           +K GE +HKIVGADK ALLKKIEELK  +AA TSTA
Sbjct: 121 MKRGETVHKIVGADKSALLKKIEELKTPTAATTSTA 122

[Close]

BLAST of Lag0004425 vs. NCBI nr
Match: XP_022951328.1 (thioredoxin H-type-like [Cucurbita moschata] >KAG6585569.1 hypothetical protein SDJN03_18302, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 193.4 bits (490), Expect = 1.5e-45
Identity = 101/155 (65.16%), Postives = 109/155 (70.32%), Query Frame = 0


Query: 1   MAEEGQVIACHKKAEWDAQLTKAKESGKLIVVDFTASWCGPCRAIAPYFSELAKNHSSVI 60
           MAEEGQVIACHKKAEWDA L KAKESGKLIVVDFTASWCGPCR IAPYFSELAKNH  V+
Sbjct: 1   MAEEGQVIACHKKAEWDAHLAKAKESGKLIVVDFTASWCGPCRTIAPYFSELAKNHPGVV 60

Query: 61  FLKVDVDELNDIAEYSSMHALELLSFDSIPVFPPSNNGFCFSLQTIAKEWEIDAMPTFVF 120
           FLKVDVDELN                                  T+A EWEI+AMPTFVF
Sbjct: 61  FLKVDVDELN----------------------------------TVATEWEINAMPTFVF 120

Query: 121 LKGGEKIHKIVGADKVALLKKIEELKASAAATSTA 156
           +K GE +HKIVGADK ALLKKIEELK   AAT+++
Sbjct: 121 MKRGETVHKIVGADKSALLKKIEELKTPTAATTSS 121

[Close]

BLAST of Lag0004425 vs. NCBI nr
Match: XP_023002551.1 (thioredoxin H-type-like [Cucurbita maxima])

HSP 1 Score: 193.4 bits (490), Expect = 1.5e-45
Identity = 104/156 (66.67%), Postives = 110/156 (70.51%), Query Frame = 0


Query: 1   MAEEGQVIACHKKAEWDAQLTKAKESGKLIVVDFTASWCGPCRAIAPYFSELAKNHSSVI 60
           MAEEGQVIACHKKAEWDA L KAKESGKLIVVDFTASWCGPCR IAPYFSELAKNH  V+
Sbjct: 1   MAEEGQVIACHKKAEWDAHLAKAKESGKLIVVDFTASWCGPCRTIAPYFSELAKNHPGVV 60

Query: 61  FLKVDVDELNDIAEYSSMHALELLSFDSIPVFPPSNNGFCFSLQTIAKEWEIDAMPTFVF 120
           FLKVDVDELN                                  T+A EWEI+AMPTFVF
Sbjct: 61  FLKVDVDELN----------------------------------TVATEWEINAMPTFVF 120

Query: 121 LKGGEKIHKIVGADKVALLKKIEELKA-SAAATSTA 156
           +K GE +HKIVGADK ALLKKIEELK  +AA TSTA
Sbjct: 121 MKRGETVHKIVGADKSALLKKIEELKTPTAATTSTA 122

[Close]

BLAST of Lag0004425 vs. NCBI nr
Match: XP_008444759.1 (PREDICTED: thioredoxin H1-like [Cucumis melo])

HSP 1 Score: 189.1 bits (479), Expect = 2.8e-44
Identity = 101/156 (64.74%), Postives = 110/156 (70.51%), Query Frame = 0


Query: 1   MAEEGQVIACHKKAEWDAQLTKAKESGKLIVVDFTASWCGPCRAIAPYFSELAKNHSSVI 60
           M EEGQVIACHK++EWDA L KAKESGKL+VVDFTASWCGPCR IAPYFSELAKNH  V+
Sbjct: 1   MGEEGQVIACHKQSEWDALLAKAKESGKLVVVDFTASWCGPCRTIAPYFSELAKNHPGVM 60

Query: 61  FLKVDVDELNDIAEYSSMHALELLSFDSIPVFPPSNNGFCFSLQTIAKEWEIDAMPTFVF 120
           FLKVDVDELN                                   IA EW+I+AMPTFVF
Sbjct: 61  FLKVDVDELN----------------------------------AIASEWKINAMPTFVF 120

Query: 121 LKGGEKIHKIVGADKVALLKKIEELKAS-AAATSTA 156
           +KGGE +HKIVGAD+ ALLKKIEELK S AAATSTA
Sbjct: 121 VKGGETLHKIVGADRAALLKKIEELKTSTAAATSTA 122

[Close]

BLAST of Lag0004425 vs. NCBI nr
Match: XP_004152676.1 (thioredoxin H-type [Cucumis sativus] >KGN62636.1 hypothetical protein Csa_022403 [Cucumis sativus])

HSP 1 Score: 188.0 bits (476), Expect = 6.3e-44
Identity = 100/157 (63.69%), Postives = 110/157 (70.06%), Query Frame = 0


Query: 1   MAEEGQVIACHKKAEWDAQLTKAKESGKLIVVDFTASWCGPCRAIAPYFSELAKNHSSVI 60
           M EEGQVIACHK++EW+A L KAKESGKL+VVDFTASWCGPCR IAPYFSELAKNHS V+
Sbjct: 1   MGEEGQVIACHKQSEWEALLAKAKESGKLVVVDFTASWCGPCRTIAPYFSELAKNHSGVM 60

Query: 61  FLKVDVDELNDIAEYSSMHALELLSFDSIPVFPPSNNGFCFSLQTIAKEWEIDAMPTFVF 120
           F+KVDVDELN                                   IA EW+I AMPTFVF
Sbjct: 61  FIKVDVDELN----------------------------------AIASEWKITAMPTFVF 120

Query: 121 LKGGEKIHKIVGADKVALLKKIEELKAS--AAATSTA 156
           +KGGE +HKIVGAD+ ALLKKIEELK S  AAATSTA
Sbjct: 121 VKGGETVHKIVGADRAALLKKIEELKTSTTAAATSTA 123

[Close]

BLAST of Lag0004425 vs. ExPASy Swiss-Prot
Match: Q96419 (Thioredoxin H-type OS=Fagopyrum esculentum OX=3617 PE=3 SV=1)

HSP 1 Score: 131.0 bits (328), Expect = 1.2e-29
Identity = 69/142 (48.59%), Postives = 84/142 (59.15%), Query Frame = 0


Query: 1   MAEEGQVIACHKKAEWDAQLTKAKESGKLIVVDFTASWCGPCRAIAPYFSELAKNHSSVI 60
           MAEE QVIACH   EW+ +  KAK+SGKLIV+DFTASWCGPCR I PY SELAK    V 
Sbjct: 1   MAEEAQVIACHTVQEWNEKFQKAKDSGKLIVIDFTASWCGPCRVITPYVSELAKKFPHVA 60

Query: 61  FLKVDVDELNDIAEYSSMHALELLSFDSIPVFPPSNNGFCFSLQTIAKEWEIDAMPTFVF 120
           F KVDVD+L D+AE                                  E++++AMP+FV 
Sbjct: 61  FFKVDVDDLKDVAE----------------------------------EYKVEAMPSFVI 108

Query: 121 LKGGEKIHKIVGADKVALLKKI 143
           LK G+++ +IVGA K  LL KI
Sbjct: 121 LKEGQEVERIVGARKDELLHKI 108

[Close]

BLAST of Lag0004425 vs. ExPASy Swiss-Prot
Match: Q0D840 (Thioredoxin H1 OS=Oryza sativa subsp. japonica OX=39947 GN=TRXH PE=1 SV=1)

HSP 1 Score: 127.9 bits (320), Expect = 1.0e-28
Identity = 68/154 (44.16%), Postives = 92/154 (59.74%), Query Frame = 0


Query: 2   AEEGQVIACHKKAEWDAQLTKAKESGKLIVVDFTASWCGPCRAIAPYFSELAKNHSSVIF 61
           AEEG VIACH K E+DAQ+TKAKE+GK++++DFTASWCGPCR IAP F+E AK     +F
Sbjct: 3   AEEGVVIACHNKDEFDAQMTKAKEAGKVVIIDFTASWCGPCRFIAPVFAEYAKKFPGAVF 62

Query: 62  LKVDVDELNDIAEYSSMHALELLSFDSIPVFPPSNNGFCFSLQTIAKEWEIDAMPTFVFL 121
           LKVDVDEL ++AE                                  ++ ++AMPTF+F+
Sbjct: 63  LKVDVDELKEVAE----------------------------------KYNVEAMPTFLFI 122

Query: 122 KGGEKIHKIVGADKVALLKKIEELKASAAATSTA 156
           K G +  K+VGA K  L   I +   + AA+++A
Sbjct: 123 KDGAEADKVVGARKDDLQNTIVKHVGATAASASA 122

[Close]

BLAST of Lag0004425 vs. ExPASy Swiss-Prot
Match: A2YIW7 (Thioredoxin H-type OS=Oryza sativa subsp. indica OX=39946 GN=TRXH PE=1 SV=1)

HSP 1 Score: 127.9 bits (320), Expect = 1.0e-28
Identity = 68/154 (44.16%), Postives = 92/154 (59.74%), Query Frame = 0


Query: 2   AEEGQVIACHKKAEWDAQLTKAKESGKLIVVDFTASWCGPCRAIAPYFSELAKNHSSVIF 61
           AEEG VIACH K E+DAQ+TKAKE+GK++++DFTASWCGPCR IAP F+E AK     +F
Sbjct: 3   AEEGVVIACHNKDEFDAQMTKAKEAGKVVIIDFTASWCGPCRFIAPVFAEYAKKFPGAVF 62

Query: 62  LKVDVDELNDIAEYSSMHALELLSFDSIPVFPPSNNGFCFSLQTIAKEWEIDAMPTFVFL 121
           LKVDVDEL ++AE                                  ++ ++AMPTF+F+
Sbjct: 63  LKVDVDELKEVAE----------------------------------KYNVEAMPTFLFI 122

Query: 122 KGGEKIHKIVGADKVALLKKIEELKASAAATSTA 156
           K G +  K+VGA K  L   I +   + AA+++A
Sbjct: 123 KDGAEADKVVGARKDDLQNTIVKHVGATAASASA 122

[Close]

BLAST of Lag0004425 vs. ExPASy Swiss-Prot
Match: Q39239 (Thioredoxin H4 OS=Arabidopsis thaliana OX=3702 GN=TRX4 PE=1 SV=2)

HSP 1 Score: 127.1 bits (318), Expect = 1.7e-28
Identity = 71/142 (50.00%), Postives = 83/142 (58.45%), Query Frame = 0


Query: 2   AEEGQVIACHKKAEWDAQLTKAKESGKLIVVDFTASWCGPCRAIAPYFSELAKNH-SSVI 61
           AEEGQVI CH    W  QL KAKES KLIV+DFTASWC PCR IAP F++LAK   SS I
Sbjct: 3   AEEGQVIGCHTNDVWTVQLDKAKESNKLIVIDFTASWCPPCRMIAPIFNDLAKKFMSSAI 62

Query: 62  FLKVDVDELNDIAEYSSMHALELLSFDSIPVFPPSNNGFCFSLQTIAKEWEIDAMPTFVF 121
           F KVDVDE                                  LQ++AKE+ ++AMPTFVF
Sbjct: 63  FFKVDVDE----------------------------------LQSVAKEFGVEAMPTFVF 110

Query: 122 LKGGEKIHKIVGADKVALLKKI 143
           +K GE + K+VGA+K  L  KI
Sbjct: 123 IKAGEVVDKLVGANKEDLQAKI 110

[Close]

BLAST of Lag0004425 vs. ExPASy Swiss-Prot
Match: P29449 (Thioredoxin H-type 1 OS=Nicotiana tabacum OX=4097 PE=2 SV=1)

HSP 1 Score: 122.5 bits (306), Expect = 4.3e-27
Identity = 67/154 (43.51%), Postives = 88/154 (57.14%), Query Frame = 0


Query: 2   AEEGQVIACHKKAEWDAQLTKAKESGKLIVVDFTASWCGPCRAIAPYFSELAKNHSSVIF 61
           +EEGQV  CHK  EW+    K  E+ KL+VVDFTASWCGPCR IAP  +++AK    VIF
Sbjct: 9   SEEGQVFGCHKVEEWNEYFKKGVETKKLVVVDFTASWCGPCRFIAPILADIAKKMPHVIF 68

Query: 62  LKVDVDELNDIAEYSSMHALELLSFDSIPVFPPSNNGFCFSLQTIAKEWEIDAMPTFVFL 121
           LKVDVDE                                  L+T++ EW ++AMPTFVF+
Sbjct: 69  LKVDVDE----------------------------------LKTVSAEWSVEAMPTFVFI 126

Query: 122 KGGEKIHKIVGADKVALLKKIEELKASAAATSTA 156
           K G+++ ++VGA K  L + I  +K +A AT TA
Sbjct: 129 KDGKEVDRVVGAKKEELQQTI--VKHAAPATVTA 126

The following BLAST results are available for this feature:

BLAST of Lag0004425 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_023537338.1	5.2e-46	67.31	thioredoxin H-type-like [Cucurbita pepo subsp. pepo]	[more]
XP_022951328.1	1.5e-45	65.16	thioredoxin H-type-like [Cucurbita moschata] >KAG6585569.1 hypothetical protein ...	[more]
XP_023002551.1	1.5e-45	66.67	thioredoxin H-type-like [Cucurbita maxima]	[more]
XP_008444759.1	2.8e-44	64.74	PREDICTED: thioredoxin H1-like [Cucumis melo]	[more]
XP_004152676.1	6.3e-44	63.69	thioredoxin H-type [Cucumis sativus] >KGN62636.1 hypothetical protein Csa_022403...	[more]

BLAST of Lag0004425 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
Q96419	1.2e-29	48.59	Thioredoxin H-type OS=Fagopyrum esculentum OX=3617 PE=3 SV=1	[more]
Q0D840	1.0e-28	44.16	Thioredoxin H1 OS=Oryza sativa subsp. japonica OX=39947 GN=TRXH PE=1 SV=1	[more]
A2YIW7	1.0e-28	44.16	Thioredoxin H-type OS=Oryza sativa subsp. indica OX=39946 GN=TRXH PE=1 SV=1	[more]
Q39239	1.7e-28	50.00	Thioredoxin H4 OS=Arabidopsis thaliana OX=3702 GN=TRX4 PE=1 SV=2	[more]
P29449	4.3e-27	43.51	Thioredoxin H-type 1 OS=Nicotiana tabacum OX=4097 PE=2 SV=1	[more]

InterPro

Analysis Name: InterPro Annotations of Sponge gourd (AG-4) v1
Date Performed: 2022-08-01


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
None	No IPR available	PRINTS	PR00421	THIOREDOXIN	coord: 30..38 score: 53.21 coord: 38..47 score: 67.0 coord: 111..122 score: 33.01
None	No IPR available	GENE3D	3.40.30.10	Glutaredoxin	coord: 1..152 e-value: 3.1E-39 score: 135.7
None	No IPR available	PANTHER	PTHR10438	THIOREDOXIN	coord: 1..83 coord: 103..148
None	No IPR available	PANTHER	PTHR10438:SF399	THIOREDOXIN	coord: 1..83 coord: 103..148
None	No IPR available	CDD	cd02947	TRX_family	coord: 18..141 e-value: 3.46047E-29 score: 100.71
IPR013766	Thioredoxin domain	PFAM	PF00085	Thioredoxin	coord: 8..76 e-value: 1.9E-17 score: 63.1
IPR013766	Thioredoxin domain	PROSITE	PS51352	THIOREDOXIN_2	coord: 1..147 score: 11.681131
IPR005746	Thioredoxin	PIRSF	PIRSF000077	Thioredoxin	coord: 102..147 e-value: 1.3E-5 score: 23.1 coord: 7..88 e-value: 1.3E-17 score: 61.7
IPR017937	Thioredoxin, conserved site	PROSITE	PS00194	THIOREDOXIN_1	coord: 31..49
IPR036249	Thioredoxin-like superfamily	SUPERFAMILY	52833	Thioredoxin-like	coord: 5..147

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
Lag0004425.1	Lag0004425.1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0006662	glycerol ether metabolic process
molecular_function	GO:0015035	protein-disulfide reductase activity