Lag0004396 (gene) Sponge gourd (AG‐4) v1

Overview


Name	Lag0004396
Type	gene
Organism	Luffa acutangula (Sponge gourd (AG‐4) v1)
Description	CBS domain-containing protein CBSX3, mitochondrial-like
Location	chr6: 3494436 .. 3496235 (+)
RNA-Seq Expression	Lag0004396
Synteny	Lag0004396

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonCDSpolypeptide

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGCAGGGGATCGTGAGAGCAGTACGATCGTGGCAAGAGACGCTGAAGATCGCGATCACGCAGCACTCGTGCAGGAGAGAAACGAATCAAGTGGAAAAGCTTCGGGAAGGATCGGAATTAGGAGGCTCGTCTTCTCCGCTGAAAGGTTTGGAGAATATTACTGTGGCGGAGATCGTTTTGAGAAAGGGAGATGGAAGCATCGGCTCCTCCTGGTTATCCTGCCGCGCGGATGATTCCGCCATTGATGCCGTGCAAAATGTAGTGAGTTTCGTTGAATTCGAATTTTCCAAAAGCTCGAAAGCCTTTTCTCGGAAGATCGAGGACTTTGATCTTTATTGATTTTGGTTGCAGATGGCAAGAAATAACATTGGATCGTTGGTGGTGCTGAAGCCTGAAGGACAACATATTGCTGGAATCGTCACAGAACGAGGTAATTGAAAGGCCATTGATAATGCCTTATTCTATAGCAAATATTAGAGATCAATTCTGAATTCACTAATTCAGTTATCTATGGTATGTGTTTAAGTACATTGATTGTTTTGACTTTAAAACTTGATTTCAATTTGCTATTCTTATTCTTTGACTGATGTTTATTAGATGTCCCTATGACAAGTTAGGTATGAACTTTTCCACAAGTAACAAAAATTCAATGGACATCTTTGTAAATTGTGGCTTTTAAATTTGCTAAATGCCCTCTTTAACATTTTAACAAACACTATTGACCTTTTTTTTTAGAAAAACACTACGGGAATGCTGAGTGAAGTTATTTACAAAGATGTTCAATATGGAGTTATAAGTCTGTGTTTAGAATGTAGAGTTCTAATAGTAGAAATGAATTTTAAGATTTTGTCGCAGATATGGAGACGTAAATGAGACTAAAATGGAAGAAGTCTCGAAGTATAGGGACCAACATGGTATTTAACTAATAAAACAATACATTACAAAACTCTCGTCATCACATCCACATATGGTAAGCTTTAGTCCTAAAATCAGCACCCTAATGAAAATGTTAAGAACACAAAAACCCAACTGATTAATGGTTTAAAATAGGTTAAAGTGAGCATAGCTTAGCAATAATTGACATATAACTTCAACCAAGAGAGTCAAATATTCAAATCTCCCACCCCAATGTTGTTGAACCAAAAAAAAAGAAATAATAATAATGATAGTTAAACATGCGGACAAAAAATTAAATTTATAAAGAAACTTAATAAGCAAAATATATGGAGAAATGGAGTAGGTTCTCATTTGTGCTCACTATTCCATGAATATTGACCTGGTAATTGCTGTTTTCTGATGGGCCTGAGCAGACTACCTGAAGAAAATAATAGCAGATGGGAGATCTCCCATATACACAAAAGTTGGAGAAATAATGACTCGTGAGGTTACTTTCGGTCTTCCCTCAGCTACAAAACCAGATTACAAACAACAGTTCTCATTCATACATCTTTATTTTTCATGAACAGGACAAACTAGTAACCGTAACATCCGACACAAACATCCTTAAAGCAATGCAGCTCATGACAGGTAAATAATAACTACAGTGTCATCTATATTCTGAAATTACCATAAAAATGGGAGTCAGCTCCTTCTCTAGCTGCATCAACGACCATATCTAAGAGGAGAGTAATCCAGTTGAAGTTTTTGGCATTTGCAGAGAATCGCATAAGACATGTTCCTGTAATAGATGGCAAACTGGTTGGCATGATTTCTATAGTAGATGTTGTCAGAGCAGTGGTAGAGCAGCAAAATGGGGAACTCGAACGACTAAACGAGTATATAAAAGGAGAATACTATTGA

mRNA sequence

ATGCAGGGGATCGTGAGAGCAGTACGATCGTGGCAAGAGACGCTGAAGATCGCGATCACGCAGCACTCGTGCAGGAGAGAAACGAATCAAGTGGAAAAGCTTCGGGAAGGATCGGAATTAGGAGGCTCGTCTTCTCCGCTGAAAGGTTTGGAGAATATTACTGTGGCGGAGATCGTTTTGAGAAAGGGAGATGGAAGCATCGGCTCCTCCTGGTTATCCTGCCGCGCGGATGATTCCGCCATTGATGCCGTGCAAAATGTAATGGCAAGAAATAACATTGGATCGTTGGTGGTGCTGAAGCCTGAAGGACAACATATTGCTGGAATCGTCACAGAACGAGACTACCTGAAGAAAATAATAGCAGATGGGAGATCTCCCATATACACAAAAGTTGGAGAAATAATGACTCGTGAGGACAAACTAGTAACCGTAACATCCGACACAAACATCCTTAAAGCAATGCAGCTCATGACAGAGAATCGCATAAGACATGTTCCTGTAATAGATGGCAAACTGGTTGGCATGATTTCTATAGTAGATGTTGTCAGAGCAGTGGTAGAGCAGCAAAATGGGGAACTCGAACGACTAAACGAGTATATAAAAGGAGAATACTATTGA

Coding sequence (CDS)

ATGCAGGGGATCGTGAGAGCAGTACGATCGTGGCAAGAGACGCTGAAGATCGCGATCACGCAGCACTCGTGCAGGAGAGAAACGAATCAAGTGGAAAAGCTTCGGGAAGGATCGGAATTAGGAGGCTCGTCTTCTCCGCTGAAAGGTTTGGAGAATATTACTGTGGCGGAGATCGTTTTGAGAAAGGGAGATGGAAGCATCGGCTCCTCCTGGTTATCCTGCCGCGCGGATGATTCCGCCATTGATGCCGTGCAAAATGTAATGGCAAGAAATAACATTGGATCGTTGGTGGTGCTGAAGCCTGAAGGACAACATATTGCTGGAATCGTCACAGAACGAGACTACCTGAAGAAAATAATAGCAGATGGGAGATCTCCCATATACACAAAAGTTGGAGAAATAATGACTCGTGAGGACAAACTAGTAACCGTAACATCCGACACAAACATCCTTAAAGCAATGCAGCTCATGACAGAGAATCGCATAAGACATGTTCCTGTAATAGATGGCAAACTGGTTGGCATGATTTCTATAGTAGATGTTGTCAGAGCAGTGGTAGAGCAGCAAAATGGGGAACTCGAACGACTAAACGAGTATATAAAAGGAGAATACTATTGA

Protein sequence

MQGIVRAVRSWQETLKIAITQHSCRRETNQVEKLREGSELGGSSSPLKGLENITVAEIVLRKGDGSIGSSWLSCRADDSAIDAVQNVMARNNIGSLVVLKPEGQHIAGIVTERDYLKKIIADGRSPIYTKVGEIMTREDKLVTVTSDTNILKAMQLMTENRIRHVPVIDGKLVGMISIVDVVRAVVEQQNGELERLNEYIKGEYY

Homology

[Close]

BLAST of Lag0004396 vs. NCBI nr
Match: XP_023538057.1 (CBS domain-containing protein CBSX3, mitochondrial-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 363.2 bits (931), Expect = 1.4e-96
Identity = 190/205 (92.68%), Postives = 197/205 (96.10%), Query Frame = 0


Query: 1   MQGIVRAVRSWQETLKIAITQHSCRRETNQVEKLREGSELGGSSSPLKGLENITVAEIVL 60
           MQGIVRAVRSWQETLKIAI+QHSCRRETNQVEKL EGS+LG SSS LKGLENI+VAEIV 
Sbjct: 1   MQGIVRAVRSWQETLKIAISQHSCRRETNQVEKLFEGSDLGASSSMLKGLENISVAEIVS 60

Query: 61  RKGDGSIGSSWLSCRADDSAIDAVQNVMARNNIGSLVVLKPEGQHIAGIVTERDYLKKII 120
            KGDGSIGS WLSCRA+D+AIDAVQN MARNNIGSLVVLKPEGQHIAGIVTERDYLKKII
Sbjct: 61  SKGDGSIGSPWLSCRANDTAIDAVQN-MARNNIGSLVVLKPEGQHIAGIVTERDYLKKII 120

Query: 121 ADGRSPIYTKVGEIMTREDKLVTVTSDTNILKAMQLMTENRIRHVPVIDGKLVGMISIVD 180
           ADGRSPIYTKVGEIMT EDKLV+VTSDTNILKAMQLMTENRIRHVPVIDGKLVGMISIVD
Sbjct: 121 ADGRSPIYTKVGEIMTSEDKLVSVTSDTNILKAMQLMTENRIRHVPVIDGKLVGMISIVD 180

Query: 181 VVRAVVEQQNGELERLNEYIKGEYY 206
           VVRAVVEQQNGEL+RLNEYIKGEYY
Sbjct: 181 VVRAVVEQQNGELKRLNEYIKGEYY 204

[Close]

BLAST of Lag0004396 vs. NCBI nr
Match: XP_023002498.1 (CBS domain-containing protein CBSX3, mitochondrial-like [Cucurbita maxima])

HSP 1 Score: 361.3 bits (926), Expect = 5.5e-96
Identity = 187/205 (91.22%), Postives = 197/205 (96.10%), Query Frame = 0


Query: 1   MQGIVRAVRSWQETLKIAITQHSCRRETNQVEKLREGSELGGSSSPLKGLENITVAEIVL 60
           MQG+VRAVRSWQETLKIAI+QHSCRRETNQVEKL +GS++G SSS LKGLENI+VAEI+ 
Sbjct: 1   MQGMVRAVRSWQETLKIAISQHSCRRETNQVEKLFKGSDIGASSSMLKGLENISVAEIIS 60

Query: 61  RKGDGSIGSSWLSCRADDSAIDAVQNVMARNNIGSLVVLKPEGQHIAGIVTERDYLKKII 120
            KGDGSIGS WLSCRADD+AIDAVQN MARNNIGSLVVLKPEGQHIAGIVTERDYLKKII
Sbjct: 61  SKGDGSIGSPWLSCRADDTAIDAVQN-MARNNIGSLVVLKPEGQHIAGIVTERDYLKKII 120

Query: 121 ADGRSPIYTKVGEIMTREDKLVTVTSDTNILKAMQLMTENRIRHVPVIDGKLVGMISIVD 180
           ADGRSPIYTKVGEIMT EDKLV+VTSDTNILKAMQLMTENRIRHVPVIDGKLVGMISIVD
Sbjct: 121 ADGRSPIYTKVGEIMTSEDKLVSVTSDTNILKAMQLMTENRIRHVPVIDGKLVGMISIVD 180

Query: 181 VVRAVVEQQNGELERLNEYIKGEYY 206
           VVRAVVEQQNGEL+RLNEYIKGEYY
Sbjct: 181 VVRAVVEQQNGELKRLNEYIKGEYY 204

[Close]

BLAST of Lag0004396 vs. NCBI nr
Match: XP_022951679.1 (CBS domain-containing protein CBSX3, mitochondrial-like [Cucurbita moschata])

HSP 1 Score: 359.4 bits (921), Expect = 2.1e-95
Identity = 189/205 (92.20%), Postives = 195/205 (95.12%), Query Frame = 0


Query: 1   MQGIVRAVRSWQETLKIAITQHSCRRETNQVEKLREGSELGGSSSPLKGLENITVAEIVL 60
           MQGIVRAVRSWQETLKIAI+QHSCRRETNQVEKL +GS LG SSS LKGLENI+VAEIV 
Sbjct: 1   MQGIVRAVRSWQETLKIAISQHSCRRETNQVEKLFKGSNLGASSSMLKGLENISVAEIVS 60

Query: 61  RKGDGSIGSSWLSCRADDSAIDAVQNVMARNNIGSLVVLKPEGQHIAGIVTERDYLKKII 120
            KGDGSIGS WLSCRADD+AI AVQN MARNNIGSLVVLKPEGQHIAGIVTERDYLKKII
Sbjct: 61  SKGDGSIGSPWLSCRADDTAIVAVQN-MARNNIGSLVVLKPEGQHIAGIVTERDYLKKII 120

Query: 121 ADGRSPIYTKVGEIMTREDKLVTVTSDTNILKAMQLMTENRIRHVPVIDGKLVGMISIVD 180
           ADGRSPIYTKVGEIMT EDKLV+VTSDTNILKAMQLMTENRIRHVPVIDGKLVGMISIVD
Sbjct: 121 ADGRSPIYTKVGEIMTSEDKLVSVTSDTNILKAMQLMTENRIRHVPVIDGKLVGMISIVD 180

Query: 181 VVRAVVEQQNGELERLNEYIKGEYY 206
           VVRAVVEQQNGEL+RLNEYIKGEYY
Sbjct: 181 VVRAVVEQQNGELKRLNEYIKGEYY 204

[Close]

BLAST of Lag0004396 vs. NCBI nr
Match: KAG6585586.1 (CBS domain-containing protein CBSX3, mitochondrial, partial [Cucurbita argyrosperma subsp. sororia] >KAG7020501.1 CBS domain-containing protein CBSX3, mitochondrial, partial [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 357.8 bits (917), Expect = 6.1e-95
Identity = 189/205 (92.20%), Postives = 194/205 (94.63%), Query Frame = 0


Query: 1   MQGIVRAVRSWQETLKIAITQHSCRRETNQVEKLREGSELGGSSSPLKGLENITVAEIVL 60
           MQ IVRAVRSWQETLKIAI+QHSCRRETNQVEKL EGS LG SSS LKGLENI VAEIV 
Sbjct: 1   MQVIVRAVRSWQETLKIAISQHSCRRETNQVEKLFEGSNLGASSSILKGLENILVAEIVS 60

Query: 61  RKGDGSIGSSWLSCRADDSAIDAVQNVMARNNIGSLVVLKPEGQHIAGIVTERDYLKKII 120
            KGDGSIGS WLSCRA+D+AIDAVQN MARNNIGSLVVLKPEGQHIAGIVTERDYLKKII
Sbjct: 61  SKGDGSIGSPWLSCRANDTAIDAVQN-MARNNIGSLVVLKPEGQHIAGIVTERDYLKKII 120

Query: 121 ADGRSPIYTKVGEIMTREDKLVTVTSDTNILKAMQLMTENRIRHVPVIDGKLVGMISIVD 180
           ADGRSPIYTKVGEIMT EDKLV+VTSDTNILKAMQLMTENRIRHVPVIDGKLVGMISIVD
Sbjct: 121 ADGRSPIYTKVGEIMTSEDKLVSVTSDTNILKAMQLMTENRIRHVPVIDGKLVGMISIVD 180

Query: 181 VVRAVVEQQNGELERLNEYIKGEYY 206
           VVRAVVEQQNGEL+RLNEYIKGEYY
Sbjct: 181 VVRAVVEQQNGELKRLNEYIKGEYY 204

[Close]

BLAST of Lag0004396 vs. NCBI nr
Match: XP_022961868.1 (CBS domain-containing protein CBSX3, mitochondrial-like isoform X1 [Cucurbita moschata])

HSP 1 Score: 354.8 bits (909), Expect = 5.1e-94
Identity = 182/205 (88.78%), Postives = 191/205 (93.17%), Query Frame = 0


Query: 1   MQGIVRAVRSWQETLKIAITQHSCRRETNQVEKLREGSELGGSSSPLKGLENITVAEIVL 60
           MQGIVRA+RSWQETLKIA + HSCRRETNQVEK+ EGSE  GSSSPLKGLENITVAEIV 
Sbjct: 1   MQGIVRAIRSWQETLKIANSHHSCRRETNQVEKILEGSESRGSSSPLKGLENITVAEIVS 60

Query: 61  RKGDGSIGSSWLSCRADDSAIDAVQNVMARNNIGSLVVLKPEGQHIAGIVTERDYLKKII 120
           R GDGSIGSSW SC ADD+AID VQNVMARNN+GSLVVLKPEGQHIAGIVTE DYLKKII
Sbjct: 61  RNGDGSIGSSWFSCSADDAAIDTVQNVMARNNVGSLVVLKPEGQHIAGIVTEGDYLKKII 120

Query: 121 ADGRSPIYTKVGEIMTREDKLVTVTSDTNILKAMQLMTENRIRHVPVIDGKLVGMISIVD 180
           ADGR PIYTKVGEIMT EDKLVTVTSDTNILKAMQLMTENRIRHVPVIDGKLVG+IS+VD
Sbjct: 121 ADGRCPIYTKVGEIMTCEDKLVTVTSDTNILKAMQLMTENRIRHVPVIDGKLVGIISVVD 180

Query: 181 VVRAVVEQQNGELERLNEYIKGEYY 206
           VV+AVV+QQNGELERLNEYIKGE Y
Sbjct: 181 VVKAVVKQQNGELERLNEYIKGESY 205

[Close]

BLAST of Lag0004396 vs. ExPASy Swiss-Prot
Match: Q9LEV3 (CBS domain-containing protein CBSX3, mitochondrial OS=Arabidopsis thaliana OX=3702 GN=CBSX3 PE=1 SV=1)

HSP 1 Score: 176.4 bits (446), Expect = 3.3e-43
Identity = 102/210 (48.57%), Postives = 143/210 (68.10%), Query Frame = 0


Query: 1   MQGIVRAVRSWQETLKIAITQHSCRRETNQVEKLREGSELGGSSSPLK----GLENITVA 60
           MQG++R+  S    +K ++ QH   R  N   +         S+ P +    G E+ T++
Sbjct: 1   MQGVIRSFVSGGNVVKGSVLQH--LRVINPAIQPSVFCSRSESTQPARMEESGFESTTIS 60

Query: 61  EIVLRKGDGSIGSSWLSCRADDSAIDAVQNVMARNNIGSLVVLKP-EGQHIAGIVTERDY 120
           +++  KG  + G SWL C  DD+  DAV++ M ++N+G+LVV+KP E Q +AGI+TERDY
Sbjct: 61  DVMKSKGKSADG-SWLWCTTDDTVYDAVKS-MTQHNVGALVVVKPGEQQALAGIITERDY 120

Query: 121 LKKIIADGRSPIYTKVGEIMTREDKLVTVTSDTNILKAMQLMTENRIRHVPVIDGK-LVG 180
           L+KII  GRS   TKVG+IMT E+KL+TVT +T +L+AMQLMT+NRIRH+PVI  K ++G
Sbjct: 121 LRKIIVQGRSSKSTKVGDIMTEENKLITVTPETKVLRAMQLMTDNRIRHIPVIKDKGMIG 180

Query: 181 MISIVDVVRAVVEQQNGELERLNEYIKGEY 205
           M+SI DVVRAVV +   EL+RLN YI+G Y
Sbjct: 181 MVSIGDVVRAVVHEHREELQRLNAYIQGGY 206

[Close]

BLAST of Lag0004396 vs. ExPASy Swiss-Prot
Match: P32987 (Uncharacterized 17.7 kDa protein in bps2 3'region OS=Acidianus ambivalens OX=2283 PE=4 SV=1)

HSP 1 Score: 69.3 bits (168), Expect = 5.7e-11
Identity = 43/117 (36.75%), Postives = 70/117 (59.83%), Query Frame = 0


Query: 88  MARNNIGSLVVLKPEGQHIAGIVTERDYLKKIIADGRSPIYTKVGEIMTREDKLVTVTSD 147
           M  +N+GSLVV+  + + + GI+TERD +K   A     I + V + MT++ K   VT D
Sbjct: 29  MKEHNLGSLVVIDSQNR-VVGIITERDIVK---AASNRDIDSPVEKYMTKDVK--GVTED 88

Query: 148 TNILKAMQLMTENRIRHVPVI--DGKLVGMISIVDVVRAVVEQQNGELERLNEYIKG 203
           T +  A+ +M  N  RH+P+I  +GKL G++SI D+ RA+++    +  +  E +KG
Sbjct: 89  TEVTDALDIMLNNGFRHLPIIKSNGKLYGIVSIRDLARALLDVHTMQFGKPAEEVKG 139

[Close]

BLAST of Lag0004396 vs. ExPASy Swiss-Prot
Match: P50100 (Uncharacterized protein MK0525 OS=Methanopyrus kandleri (strain AV19 / DSM 6324 / JCM 9639 / NBRC 100938) OX=190192 GN=MK0525 PE=4 SV=2)

HSP 1 Score: 67.0 bits (162), Expect = 2.8e-10
Identity = 40/130 (30.77%), Postives = 80/130 (61.54%), Query Frame = 0


Query: 88  MARNNIGSLVVLKPEGQHIAGIVTERDYLKKIIADGRSPIYTKVGEIMTREDKLVTVTSD 147
           M  + IGS+V++  + + I GI+TERD + K+++ G++P      +IM++   ++TV  D
Sbjct: 30  MREHGIGSVVIVNEKDEPI-GIITERDLVIKVVSQGKNPDEVIARDIMSQ--PVITVEED 89

Query: 148 TNILKAMQLMTENRIRHVPVID--GKLVGMISIVDVVR------AVVEQQN-------GE 203
             + +A++LM +  IR +P++D  GKL+G++++ D+++      A +E++         E
Sbjct: 90  MEVNEAVKLMVDKGIRRLPIVDDNGKLIGIVTMQDILQVEPYLVATIEEEMKKFQEELEE 149

[Close]

BLAST of Lag0004396 vs. ExPASy Swiss-Prot
Match: Q9SJQ5 (CBS domain-containing protein CBSCBSPB2 OS=Arabidopsis thaliana OX=3702 GN=CBSCBSPB2 PE=2 SV=1)

HSP 1 Score: 61.6 bits (148), Expect = 1.2e-08
Identity = 36/128 (28.12%), Postives = 70/128 (54.69%), Query Frame = 0


Query: 69  SSWLSCRADDSAIDAVQNVMARNNIGSLVVLKPEGQHIAGIVTERDYLKKIIADGRSPIY 128
           S  L+     +  DA + + AR      V+L      ++GIVT++D   ++IA+G  P +
Sbjct: 68  SKALTINEGTTVFDACRRMAARR--VDAVLLTDSSALLSGIVTDKDIATRVIAEGLRPEH 127

Query: 129 TKVGEIMTREDKLVTVTSDTNILKAMQLMTENRIRHVPVID-GKLVGMISIVDVVRAVVE 188
           T V ++MTR    + VTSD+  ++A+Q M + + RH+PV++ G+++ ++ I   +   + 
Sbjct: 128 TLVSKVMTRNP--IFVTSDSLAIEALQKMVQGKFRHLPVVENGEVIALLDITKCLYDAIS 187

Query: 189 QQNGELER 196
           +     E+
Sbjct: 188 RMEKAAEQ 191

[Close]

BLAST of Lag0004396 vs. ExPASy Swiss-Prot
Match: P42851 (Inosine-5'-monophosphate dehydrogenase OS=Pyrococcus furiosus (strain ATCC 43587 / DSM 3638 / JCM 8422 / Vc1) OX=186497 GN=guaB PE=3 SV=1)

HSP 1 Score: 60.8 bits (146), Expect = 2.0e-08
Identity = 44/125 (35.20%), Postives = 72/125 (57.60%), Query Frame = 0


Query: 76  ADDSAIDAVQNVMARNNIGSLVVLKPEGQHIAGIVTERDYLKKIIADGRSPIYTKVGEIM 135
           A D  ID    +M ++ I  L V+  E   + GI+T++D   +   +GR+     V E+M
Sbjct: 107 APDETIDYALFLMEKHGIDGLPVV--EEDRVVGIITKKDIAAR---EGRT-----VKELM 166

Query: 136 TREDKLVTVTSDTNILKAMQLMTENRIRHVPVI--DGKLVGMISIVDVV-----RAVVEQ 194
           TRE  ++TV    ++ +A+++M ENRI  +PV+  DGKLVG+I++ D+V     +  V  
Sbjct: 167 TRE--VITVPESVDVEEALKIMMENRIDRLPVVNEDGKLVGLITMSDLVARKKYKNAVRN 219

[Close]

BLAST of Lag0004396 vs. ExPASy TrEMBL
Match: A0A6J1KTQ7 (CBS domain-containing protein CBSX3, mitochondrial-like OS=Cucurbita maxima OX=3661 GN=LOC111496322 PE=4 SV=1)

HSP 1 Score: 361.3 bits (926), Expect = 2.7e-96
Identity = 187/205 (91.22%), Postives = 197/205 (96.10%), Query Frame = 0


Query: 1   MQGIVRAVRSWQETLKIAITQHSCRRETNQVEKLREGSELGGSSSPLKGLENITVAEIVL 60
           MQG+VRAVRSWQETLKIAI+QHSCRRETNQVEKL +GS++G SSS LKGLENI+VAEI+ 
Sbjct: 1   MQGMVRAVRSWQETLKIAISQHSCRRETNQVEKLFKGSDIGASSSMLKGLENISVAEIIS 60

Query: 61  RKGDGSIGSSWLSCRADDSAIDAVQNVMARNNIGSLVVLKPEGQHIAGIVTERDYLKKII 120
            KGDGSIGS WLSCRADD+AIDAVQN MARNNIGSLVVLKPEGQHIAGIVTERDYLKKII
Sbjct: 61  SKGDGSIGSPWLSCRADDTAIDAVQN-MARNNIGSLVVLKPEGQHIAGIVTERDYLKKII 120

Query: 121 ADGRSPIYTKVGEIMTREDKLVTVTSDTNILKAMQLMTENRIRHVPVIDGKLVGMISIVD 180
           ADGRSPIYTKVGEIMT EDKLV+VTSDTNILKAMQLMTENRIRHVPVIDGKLVGMISIVD
Sbjct: 121 ADGRSPIYTKVGEIMTSEDKLVSVTSDTNILKAMQLMTENRIRHVPVIDGKLVGMISIVD 180

Query: 181 VVRAVVEQQNGELERLNEYIKGEYY 206
           VVRAVVEQQNGEL+RLNEYIKGEYY
Sbjct: 181 VVRAVVEQQNGELKRLNEYIKGEYY 204

[Close]

BLAST of Lag0004396 vs. ExPASy TrEMBL
Match: A0A6J1GJI0 (CBS domain-containing protein CBSX3, mitochondrial-like OS=Cucurbita moschata OX=3662 GN=LOC111454421 PE=4 SV=1)

HSP 1 Score: 359.4 bits (921), Expect = 1.0e-95
Identity = 189/205 (92.20%), Postives = 195/205 (95.12%), Query Frame = 0


Query: 1   MQGIVRAVRSWQETLKIAITQHSCRRETNQVEKLREGSELGGSSSPLKGLENITVAEIVL 60
           MQGIVRAVRSWQETLKIAI+QHSCRRETNQVEKL +GS LG SSS LKGLENI+VAEIV 
Sbjct: 1   MQGIVRAVRSWQETLKIAISQHSCRRETNQVEKLFKGSNLGASSSMLKGLENISVAEIVS 60

Query: 61  RKGDGSIGSSWLSCRADDSAIDAVQNVMARNNIGSLVVLKPEGQHIAGIVTERDYLKKII 120
            KGDGSIGS WLSCRADD+AI AVQN MARNNIGSLVVLKPEGQHIAGIVTERDYLKKII
Sbjct: 61  SKGDGSIGSPWLSCRADDTAIVAVQN-MARNNIGSLVVLKPEGQHIAGIVTERDYLKKII 120

Query: 121 ADGRSPIYTKVGEIMTREDKLVTVTSDTNILKAMQLMTENRIRHVPVIDGKLVGMISIVD 180
           ADGRSPIYTKVGEIMT EDKLV+VTSDTNILKAMQLMTENRIRHVPVIDGKLVGMISIVD
Sbjct: 121 ADGRSPIYTKVGEIMTSEDKLVSVTSDTNILKAMQLMTENRIRHVPVIDGKLVGMISIVD 180

Query: 181 VVRAVVEQQNGELERLNEYIKGEYY 206
           VVRAVVEQQNGEL+RLNEYIKGEYY
Sbjct: 181 VVRAVVEQQNGELKRLNEYIKGEYY 204

[Close]

BLAST of Lag0004396 vs. ExPASy TrEMBL
Match: A0A6J1HD27 (CBS domain-containing protein CBSX3, mitochondrial-like isoform X1 OS=Cucurbita moschata OX=3662 GN=LOC111462506 PE=4 SV=1)

HSP 1 Score: 354.8 bits (909), Expect = 2.5e-94
Identity = 182/205 (88.78%), Postives = 191/205 (93.17%), Query Frame = 0


Query: 1   MQGIVRAVRSWQETLKIAITQHSCRRETNQVEKLREGSELGGSSSPLKGLENITVAEIVL 60
           MQGIVRA+RSWQETLKIA + HSCRRETNQVEK+ EGSE  GSSSPLKGLENITVAEIV 
Sbjct: 1   MQGIVRAIRSWQETLKIANSHHSCRRETNQVEKILEGSESRGSSSPLKGLENITVAEIVS 60

Query: 61  RKGDGSIGSSWLSCRADDSAIDAVQNVMARNNIGSLVVLKPEGQHIAGIVTERDYLKKII 120
           R GDGSIGSSW SC ADD+AID VQNVMARNN+GSLVVLKPEGQHIAGIVTE DYLKKII
Sbjct: 61  RNGDGSIGSSWFSCSADDAAIDTVQNVMARNNVGSLVVLKPEGQHIAGIVTEGDYLKKII 120

Query: 121 ADGRSPIYTKVGEIMTREDKLVTVTSDTNILKAMQLMTENRIRHVPVIDGKLVGMISIVD 180
           ADGR PIYTKVGEIMT EDKLVTVTSDTNILKAMQLMTENRIRHVPVIDGKLVG+IS+VD
Sbjct: 121 ADGRCPIYTKVGEIMTCEDKLVTVTSDTNILKAMQLMTENRIRHVPVIDGKLVGIISVVD 180

Query: 181 VVRAVVEQQNGELERLNEYIKGEYY 206
           VV+AVV+QQNGELERLNEYIKGE Y
Sbjct: 181 VVKAVVKQQNGELERLNEYIKGESY 205

[Close]

BLAST of Lag0004396 vs. ExPASy TrEMBL
Match: A0A6J1K7H6 (CBS domain-containing protein CBSX3, mitochondrial-like isoform X1 OS=Cucurbita maxima OX=3661 GN=LOC111491856 PE=4 SV=1)

HSP 1 Score: 349.0 bits (894), Expect = 1.4e-92
Identity = 179/205 (87.32%), Postives = 190/205 (92.68%), Query Frame = 0


Query: 1   MQGIVRAVRSWQETLKIAITQHSCRRETNQVEKLREGSELGGSSSPLKGLENITVAEIVL 60
           MQ IV+A+RSWQETLKIA +QHSCRRETNQVEK  EGSE  GSSSPLKG+ENITVAEIV 
Sbjct: 43  MQAIVKALRSWQETLKIANSQHSCRRETNQVEKFLEGSESRGSSSPLKGMENITVAEIVS 102

Query: 61  RKGDGSIGSSWLSCRADDSAIDAVQNVMARNNIGSLVVLKPEGQHIAGIVTERDYLKKII 120
           RKGDGSIGSSW SC ADD+AID VQNVMARNN+GSLVVLKPEGQHIAGIVTE DYLKKII
Sbjct: 103 RKGDGSIGSSWFSCSADDAAIDTVQNVMARNNVGSLVVLKPEGQHIAGIVTEGDYLKKII 162

Query: 121 ADGRSPIYTKVGEIMTREDKLVTVTSDTNILKAMQLMTENRIRHVPVIDGKLVGMISIVD 180
           ADGR PI+TKVGEIMT EDKLVTVTSDTNILKAMQLMTENR RHVPVIDGKLVGMISIVD
Sbjct: 163 ADGRCPIFTKVGEIMTCEDKLVTVTSDTNILKAMQLMTENRTRHVPVIDGKLVGMISIVD 222

Query: 181 VVRAVVEQQNGELERLNEYIKGEYY 206
           VV+AVV+QQNGELE++NEYIKGE Y
Sbjct: 223 VVKAVVKQQNGELEQINEYIKGESY 247

[Close]

BLAST of Lag0004396 vs. ExPASy TrEMBL
Match: A0A6J1HF98 (CBS domain-containing protein CBSX3, mitochondrial-like isoform X2 OS=Cucurbita moschata OX=3662 GN=LOC111462506 PE=4 SV=1)

HSP 1 Score: 349.0 bits (894), Expect = 1.4e-92
Identity = 181/205 (88.29%), Postives = 190/205 (92.68%), Query Frame = 0


Query: 1   MQGIVRAVRSWQETLKIAITQHSCRRETNQVEKLREGSELGGSSSPLKGLENITVAEIVL 60
           MQGIVRA+RSWQETLKIA + HSCRRETNQVEK+ EGSE  GSSSPLKGLENITVAEIV 
Sbjct: 1   MQGIVRAIRSWQETLKIANSHHSCRRETNQVEKILEGSESRGSSSPLKGLENITVAEIVS 60

Query: 61  RKGDGSIGSSWLSCRADDSAIDAVQNVMARNNIGSLVVLKPEGQHIAGIVTERDYLKKII 120
           R GDGSIGSSW SC ADD+AID VQN MARNN+GSLVVLKPEGQHIAGIVTE DYLKKII
Sbjct: 61  RNGDGSIGSSWFSCSADDAAIDTVQN-MARNNVGSLVVLKPEGQHIAGIVTEGDYLKKII 120

Query: 121 ADGRSPIYTKVGEIMTREDKLVTVTSDTNILKAMQLMTENRIRHVPVIDGKLVGMISIVD 180
           ADGR PIYTKVGEIMT EDKLVTVTSDTNILKAMQLMTENRIRHVPVIDGKLVG+IS+VD
Sbjct: 121 ADGRCPIYTKVGEIMTCEDKLVTVTSDTNILKAMQLMTENRIRHVPVIDGKLVGIISVVD 180

Query: 181 VVRAVVEQQNGELERLNEYIKGEYY 206
           VV+AVV+QQNGELERLNEYIKGE Y
Sbjct: 181 VVKAVVKQQNGELERLNEYIKGESY 204

[Close]

BLAST of Lag0004396 vs. TAIR 10
Match: AT1G47271.1 (Cystathionine beta-synthase (CBS) family protein )

HSP 1 Score: 207.6 bits (527), Expect = 9.5e-54
Identity = 109/161 (67.70%), Postives = 137/161 (85.09%), Query Frame = 0


Query: 46  PLKGLENITVAEIVLRKGDGSIGSSWLSCRADDSAIDAVQNVMARNNIGSLVVLKP-EGQ 105
           P KGLEN+TVA+++  K D  I  +W+SCR +D+  DAV+N MA++NIGSLVVL+P + Q
Sbjct: 36  PEKGLENLTVADVLSTK-DTDI-DTWISCRTNDTVSDAVKN-MAKHNIGSLVVLEPGDQQ 95

Query: 106 HIAGIVTERDYLKKIIADGRSPIYTKVGEIMTREDKLVTVTSDTNILKAMQLMTENRIRH 165
           +IAGIVTERDY+KKII  GRS   TKVGE+MT E KLVTV+S TNI+KAMQLM+EN IRH
Sbjct: 96  YIAGIVTERDYMKKIIGAGRSSKLTKVGEVMTDESKLVTVSSGTNIIKAMQLMSENHIRH 155

Query: 166 VPVIDGKLVGMISIVDVVRAVVEQQNGELERLNEYIKGEYY 206
           VPVIDGK+VG+IS+VDVV+A+V+ QNGEL+RLNE+IKG+YY
Sbjct: 156 VPVIDGKIVGLISMVDVVKAIVDHQNGELKRLNEFIKGDYY 193

[Close]

BLAST of Lag0004396 vs. TAIR 10
Match: AT5G10860.1 (Cystathionine beta-synthase (CBS) family protein )

HSP 1 Score: 176.4 bits (446), Expect = 2.3e-44
Identity = 102/210 (48.57%), Postives = 143/210 (68.10%), Query Frame = 0


Query: 1   MQGIVRAVRSWQETLKIAITQHSCRRETNQVEKLREGSELGGSSSPLK----GLENITVA 60
           MQG++R+  S    +K ++ QH   R  N   +         S+ P +    G E+ T++
Sbjct: 1   MQGVIRSFVSGGNVVKGSVLQH--LRVINPAIQPSVFCSRSESTQPARMEESGFESTTIS 60

Query: 61  EIVLRKGDGSIGSSWLSCRADDSAIDAVQNVMARNNIGSLVVLKP-EGQHIAGIVTERDY 120
           +++  KG  + G SWL C  DD+  DAV++ M ++N+G+LVV+KP E Q +AGI+TERDY
Sbjct: 61  DVMKSKGKSADG-SWLWCTTDDTVYDAVKS-MTQHNVGALVVVKPGEQQALAGIITERDY 120

Query: 121 LKKIIADGRSPIYTKVGEIMTREDKLVTVTSDTNILKAMQLMTENRIRHVPVIDGK-LVG 180
           L+KII  GRS   TKVG+IMT E+KL+TVT +T +L+AMQLMT+NRIRH+PVI  K ++G
Sbjct: 121 LRKIIVQGRSSKSTKVGDIMTEENKLITVTPETKVLRAMQLMTDNRIRHIPVIKDKGMIG 180

Query: 181 MISIVDVVRAVVEQQNGELERLNEYIKGEY 205
           M+SI DVVRAVV +   EL+RLN YI+G Y
Sbjct: 181 MVSIGDVVRAVVHEHREELQRLNAYIQGGY 206

[Close]

BLAST of Lag0004396 vs. TAIR 10
Match: AT2G36500.1 (CBS / octicosapeptide/Phox/Bemp1 (PB1) domains-containing protein )

HSP 1 Score: 61.6 bits (148), Expect = 8.4e-10
Identity = 36/128 (28.12%), Postives = 70/128 (54.69%), Query Frame = 0


Query: 69  SSWLSCRADDSAIDAVQNVMARNNIGSLVVLKPEGQHIAGIVTERDYLKKIIADGRSPIY 128
           S  L+     +  DA + + AR      V+L      ++GIVT++D   ++IA+G  P +
Sbjct: 68  SKALTINEGTTVFDACRRMAARR--VDAVLLTDSSALLSGIVTDKDIATRVIAEGLRPEH 127

Query: 129 TKVGEIMTREDKLVTVTSDTNILKAMQLMTENRIRHVPVID-GKLVGMISIVDVVRAVVE 188
           T V ++MTR    + VTSD+  ++A+Q M + + RH+PV++ G+++ ++ I   +   + 
Sbjct: 128 TLVSKVMTRNP--IFVTSDSLAIEALQKMVQGKFRHLPVVENGEVIALLDITKCLYDAIS 187

Query: 189 QQNGELER 196
           +     E+
Sbjct: 188 RMEKAAEQ 191

[Close]

BLAST of Lag0004396 vs. TAIR 10
Match: AT3G52950.1 (CBS / octicosapeptide/Phox/Bemp1 (PB1) domains-containing protein )

HSP 1 Score: 58.9 bits (141), Expect = 5.4e-09
Identity = 35/118 (29.66%), Postives = 65/118 (55.08%), Query Frame = 0


Query: 79  SAIDAVQNVMARNNIGSLVVLKPEGQHIAGIVTERDYLKKIIADGRSPIYTKVGEIMTRE 138
           +  DA + + AR     L  L      ++GIVT++D   ++IA+G  P  T V ++MTR 
Sbjct: 80  TVFDACRRMAARRVDACL--LTDSSALLSGIVTDKDVATRVIAEGLRPDQTLVSKVMTRN 139

Query: 139 DKLVTVTSDTNILKAMQLMTENRIRHVPVID-GKLVGMISIVDVVRAVVEQQNGELER 196
              + VTSD+  L+A+Q M + + RH+PV++ G+++ ++ I   +   + +     E+
Sbjct: 140 P--IFVTSDSLALEALQKMVQGKFRHLPVVENGEVIALLDITKCLYDAISRMEKAAEQ 193

[Close]

BLAST of Lag0004396 vs. TAIR 10
Match: AT3G52950.2 (CBS / octicosapeptide/Phox/Bemp1 (PB1) domains-containing protein )

HSP 1 Score: 58.9 bits (141), Expect = 5.4e-09
Identity = 35/118 (29.66%), Postives = 65/118 (55.08%), Query Frame = 0


Query: 79  SAIDAVQNVMARNNIGSLVVLKPEGQHIAGIVTERDYLKKIIADGRSPIYTKVGEIMTRE 138
           +  DA + + AR     L  L      ++GIVT++D   ++IA+G  P  T V ++MTR 
Sbjct: 80  TVFDACRRMAARRVDACL--LTDSSALLSGIVTDKDVATRVIAEGLRPDQTLVSKVMTRN 139

Query: 139 DKLVTVTSDTNILKAMQLMTENRIRHVPVID-GKLVGMISIVDVVRAVVEQQNGELER 196
              + VTSD+  L+A+Q M + + RH+PV++ G+++ ++ I   +   + +     E+
Sbjct: 140 P--IFVTSDSLALEALQKMVQGKFRHLPVVENGEVIALLDITKCLYDAISRMEKAAEQ 193

The following BLAST results are available for this feature:

BLAST of Lag0004396 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_023538057.1	1.4e-96	92.68	CBS domain-containing protein CBSX3, mitochondrial-like [Cucurbita pepo subsp. p...	[more]
XP_023002498.1	5.5e-96	91.22	CBS domain-containing protein CBSX3, mitochondrial-like [Cucurbita maxima]	[more]
XP_022951679.1	2.1e-95	92.20	CBS domain-containing protein CBSX3, mitochondrial-like [Cucurbita moschata]	[more]
KAG6585586.1	6.1e-95	92.20	CBS domain-containing protein CBSX3, mitochondrial, partial [Cucurbita argyrospe...	[more]
XP_022961868.1	5.1e-94	88.78	CBS domain-containing protein CBSX3, mitochondrial-like isoform X1 [Cucurbita mo...	[more]

BLAST of Lag0004396 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
Q9LEV3	3.3e-43	48.57	CBS domain-containing protein CBSX3, mitochondrial OS=Arabidopsis thaliana OX=37...	[more]
P32987	5.7e-11	36.75	Uncharacterized 17.7 kDa protein in bps2 3'region OS=Acidianus ambivalens OX=228...	[more]
P50100	2.8e-10	30.77	Uncharacterized protein MK0525 OS=Methanopyrus kandleri (strain AV19 / DSM 6324 ...	[more]
Q9SJQ5	1.2e-08	28.13	CBS domain-containing protein CBSCBSPB2 OS=Arabidopsis thaliana OX=3702 GN=CBSCB...	[more]
P42851	2.0e-08	35.20	Inosine-5'-monophosphate dehydrogenase OS=Pyrococcus furiosus (strain ATCC 43587...	[more]

BLAST of Lag0004396 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A6J1KTQ7	2.7e-96	91.22	CBS domain-containing protein CBSX3, mitochondrial-like OS=Cucurbita maxima OX=3...	[more]
A0A6J1GJI0	1.0e-95	92.20	CBS domain-containing protein CBSX3, mitochondrial-like OS=Cucurbita moschata OX...	[more]
A0A6J1HD27	2.5e-94	88.78	CBS domain-containing protein CBSX3, mitochondrial-like isoform X1 OS=Cucurbita ...	[more]
A0A6J1K7H6	1.4e-92	87.32	CBS domain-containing protein CBSX3, mitochondrial-like isoform X1 OS=Cucurbita ...	[more]
A0A6J1HF98	1.4e-92	88.29	CBS domain-containing protein CBSX3, mitochondrial-like isoform X2 OS=Cucurbita ...	[more]

BLAST of Lag0004396 vs. TAIR 10


Match Name	E-value	Identity	Description
AT1G47271.1	9.5e-54	67.70	Cystathionine beta-synthase (CBS) family protein	[more]
AT5G10860.1	2.3e-44	48.57	Cystathionine beta-synthase (CBS) family protein	[more]
AT2G36500.1	8.4e-10	28.13	CBS / octicosapeptide/Phox/Bemp1 (PB1) domains-containing protein	[more]
AT3G52950.1	5.4e-09	29.66	CBS / octicosapeptide/Phox/Bemp1 (PB1) domains-containing protein	[more]
AT3G52950.2	5.4e-09	29.66	CBS / octicosapeptide/Phox/Bemp1 (PB1) domains-containing protein	[more]

InterPro

Analysis Name: InterPro Annotations of Sponge gourd (AG-4) v1
Date Performed: 2022-08-01

Position :

Zoom :

x 1


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR000644	CBS domain	SMART	SM00116	cbs_1	coord: 70..120 e-value: 1.3 score: 17.7 coord: 140..186 e-value: 1.2E-10 score: 51.4
IPR000644	CBS domain	PFAM	PF00571	CBS	coord: 73..119 e-value: 1.0E-5 score: 25.9 coord: 131..186 e-value: 1.5E-12 score: 47.7
IPR000644	CBS domain	PROSITE	PS51371	CBS	coord: 67..127 score: 9.631515
IPR000644	CBS domain	PROSITE	PS51371	CBS	coord: 135..193 score: 14.466565
None	No IPR available	GENE3D	3.10.580.10		coord: 48..204 e-value: 1.0E-42 score: 147.3
None	No IPR available	PANTHER	PTHR43080:SF12	CYSTATHIONINE BETA-SYNTHASE (CBS) FAMILY PROTEIN	coord: 1..204
None	No IPR available	PANTHER	PTHR43080	CBS DOMAIN-CONTAINING PROTEIN CBSX3, MITOCHONDRIAL	coord: 1..204
None	No IPR available	SUPERFAMILY	54631	CBS-domain pair	coord: 70..192
IPR044725	CBS domain-containing protein CBSX3, CBS domain	CDD	cd04623	CBS_pair_bac_euk	coord: 68..183 e-value: 5.292E-45 score: 143.325

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
Lag0004396.1	Lag0004396.1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
cellular_component	GO:0016021	integral component of membrane