Lag0004395 (gene) Sponge gourd (AG‐4) v1

Overview
NameLag0004395
Typegene
OrganismLuffa acutangula (Sponge gourd (AG‐4) v1)
Descriptioncell wall protein RBR3-like
Locationchr6: 3489855 .. 3491765 (-)
RNA-Seq ExpressionLag0004395
SyntenyLag0004395
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonCDSpolypeptide
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGTCATACCGACAATTTCGCTTTCGACTTCCTTGGCAATCTGTAAAAGCTTCATCTCATCCTGCAGATGAGTCATCAAGACGCAGTTCTGAGCCTACAGATGAAACCAAAGCTTCTTCTTCAGTGGCTGATACCGTGCCAAATATTTGGCATCAACCAGGCCAGTCTCCTGAGATAAAACCGGAGCAGCCTCCTCTAGCACCAGCTCAGGCAGCTGAAAAAAGTGAAACTATGCCACCTTCAAAATCTCACAAGGAAGCCAAAGTTCATGCTCAATCACCATCACATTCCCGAGCCAAAAACCAAACCCGAACGGCTTCGAAGCCTCCATCACCATCAAAAGCAATCCCTCAATCCTCAGTTGCTTCGAACAAGTCTCCATCAGCATCAGGCAAAGCCTCTCCATCTCATGATACTTCAAAGCGTTCATCACCAGCAGATAAAGCCTCTCCATCTTCAAAACCTTCATCACCAGCAGGCAAAGCCTCTCCATTTCAAAATACTTCAAAGCCTTCATCACTAGCAGGCAAAGCCTCCCCATCTAAGGATGCTTCAAAGCCTTCATCACCAGCAGGCAAGGCCTCTCCATCTCAGGATGCTTCAAAGCCTTCATCACCAGCTGCTCCTCGATCCCGAATGGCTTCGAAGCCACCGTCTCCATCTCAAACATCCAGTAAAAACCATCCAAATTCAAAACCAACATCACAATCGAGACTGAAAGCTGATTCTCAACCTCAAGTTTCTTCAATGCCGCGGTCACCATCTCGAAAACAACCATCGAAAAAAACCTCCCGAGTTCAGTCTCCATCTCATTTGTCCAGTAAACCTATTGCACAATCAACATCACCACAGCATATTGAATCTCCTGCAGCCATTGGAGACCAAACAACAAAAGAAATTGTTTCTCATCCAGCAAATCAATCGCCACAAGCAAGACCTAAAAGCAGGGAAAGTCAGTTGGAAACGAAATCAAAGCAGTCTCCAAAACCTGACTCGAAACCAGTGGAGTCCAGAGCATCAAAAGATCAGCCTGAAACCAATGAAGAGCCCACACTCACATCTAAGAACACTTCCCATCCCCATTCAAACCAGGACTCTTCTGAAATCCCAATACAGCCCAATCAAACCATTGAAAATGGTCTAGAGCCCTCTCTAGAATCACAGGAAGAGTCAAAGGAAAATAAGGAAGATCAGGCAAAAACAATCAATGCACTTCAAACCAGTGCATCAAGAAGCACATTGATCACATCTTCCGAAAGCCCTTCACCATTTGAACAAGAAGATAAGGACTCAAAACAGGAAGAAACCATGGAAGACTTATCAAAAGCTTTTCAGAAACTAAACATCAAATACTCAGACGAAGAAAATCCAAAGAGTTTCACAACACTCATCGGCGATAACAAAGGGGCTTCAATGCACGTACATTCCGGTGAAGCCAAGAGAGAGAGTTCAATCCACATCCACCGTGATTACAAGAGCGATCCAGACCAAAGCCCTGAAAGTGAAAGTTCCACAGACGACGAAGGAAACTTCAATAACGAAACACCACAAGAATCAAGAACAGAAGAGGATCCACCACTGGAGTTATACATAAACATCAACGTACAAGGTATCAACAACTCAATCCTGTCCAATACCTCATTCACTGAGAATAATCCTGGAATCAAGTTGAAATTCGTTCGAGAACCAACTAGATCTGAAGATGAATTACAGTCTCATGACGCTCGAGAGGCCAAGTATACTGCGAAACCTGCCGAGAGGCTTACATATGAGCCCACAGTAAGAAGAAGATGCCTCAGAGGGCTGTTAATGGAGTCGAGCGATTCTGAGCTCGACGATCCAGAAAAGCCCCGACGCCATGGCTGCCGCTACAGTCGAGGTTGCGAAGGAAAAGAGGTCGAAATTCTGTAG

mRNA sequence

ATGTCATACCGACAATTTCGCTTTCGACTTCCTTGGCAATCTGTAAAAGCTTCATCTCATCCTGCAGATGAGTCATCAAGACGCAGTTCTGAGCCTACAGATGAAACCAAAGCTTCTTCTTCAGTGGCTGATACCGTGCCAAATATTTGGCATCAACCAGGCCAGTCTCCTGAGATAAAACCGGAGCAGCCTCCTCTAGCACCAGCTCAGGCAGCTGAAAAAAGTGAAACTATGCCACCTTCAAAATCTCACAAGGAAGCCAAAGTTCATGCTCAATCACCATCACATTCCCGAGCCAAAAACCAAACCCGAACGGCTTCGAAGCCTCCATCACCATCAAAAGCAATCCCTCAATCCTCAGTTGCTTCGAACAAGTCTCCATCAGCATCAGGCAAAGCCTCTCCATCTCATGATACTTCAAAGCGTTCATCACCAGCAGATAAAGCCTCTCCATCTTCAAAACCTTCATCACCAGCAGGCAAAGCCTCTCCATTTCAAAATACTTCAAAGCCTTCATCACTAGCAGGCAAAGCCTCCCCATCTAAGGATGCTTCAAAGCCTTCATCACCAGCAGGCAAGGCCTCTCCATCTCAGGATGCTTCAAAGCCTTCATCACCAGCTGCTCCTCGATCCCGAATGGCTTCGAAGCCACCGTCTCCATCTCAAACATCCAGTAAAAACCATCCAAATTCAAAACCAACATCACAATCGAGACTGAAAGCTGATTCTCAACCTCAAGTTTCTTCAATGCCGCGGTCACCATCTCGAAAACAACCATCGAAAAAAACCTCCCGAGTTCAGTCTCCATCTCATTTGTCCAGTAAACCTATTGCACAATCAACATCACCACAGCATATTGAATCTCCTGCAGCCATTGGAGACCAAACAACAAAAGAAATTGTTTCTCATCCAGCAAATCAATCGCCACAAGCAAGACCTAAAAGCAGGGAAAGTCAGTTGGAAACGAAATCAAAGCAGTCTCCAAAACCTGACTCGAAACCAGTGGAGTCCAGAGCATCAAAAGATCAGCCTGAAACCAATGAAGAGCCCACACTCACATCTAAGAACACTTCCCATCCCCATTCAAACCAGGACTCTTCTGAAATCCCAATACAGCCCAATCAAACCATTGAAAATGGTCTAGAGCCCTCTCTAGAATCACAGGAAGAGTCAAAGGAAAATAAGGAAGATCAGGCAAAAACAATCAATGCACTTCAAACCAGTGCATCAAGAAGCACATTGATCACATCTTCCGAAAGCCCTTCACCATTTGAACAAGAAGATAAGGACTCAAAACAGGAAGAAACCATGGAAGACTTATCAAAAGCTTTTCAGAAACTAAACATCAAATACTCAGACGAAGAAAATCCAAAGAGTTTCACAACACTCATCGGCGATAACAAAGGGGCTTCAATGCACGTACATTCCGGTGAAGCCAAGAGAGAGAGTTCAATCCACATCCACCGTGATTACAAGAGCGATCCAGACCAAAGCCCTGAAAGTGAAAGTTCCACAGACGACGAAGGAAACTTCAATAACGAAACACCACAAGAATCAAGAACAGAAGAGGATCCACCACTGGAGTTATACATAAACATCAACGTACAAGGTATCAACAACTCAATCCTGTCCAATACCTCATTCACTGAGAATAATCCTGGAATCAAGTTGAAATTCGTTCGAGAACCAACTAGATCTGAAGATGAATTACAGTCTCATGACGCTCGAGAGGCCAAGTATACTGCGAAACCTGCCGAGAGGCTTACATATGAGCCCACAGTAAGAAGAAGATGCCTCAGAGGGCTGTTAATGGAGTCGAGCGATTCTGAGCTCGACGATCCAGAAAAGCCCCGACGCCATGGCTGCCGCTACAGTCGAGGTTGCGAAGGAAAAGAGGTCGAAATTCTGTAG

Coding sequence (CDS)

ATGTCATACCGACAATTTCGCTTTCGACTTCCTTGGCAATCTGTAAAAGCTTCATCTCATCCTGCAGATGAGTCATCAAGACGCAGTTCTGAGCCTACAGATGAAACCAAAGCTTCTTCTTCAGTGGCTGATACCGTGCCAAATATTTGGCATCAACCAGGCCAGTCTCCTGAGATAAAACCGGAGCAGCCTCCTCTAGCACCAGCTCAGGCAGCTGAAAAAAGTGAAACTATGCCACCTTCAAAATCTCACAAGGAAGCCAAAGTTCATGCTCAATCACCATCACATTCCCGAGCCAAAAACCAAACCCGAACGGCTTCGAAGCCTCCATCACCATCAAAAGCAATCCCTCAATCCTCAGTTGCTTCGAACAAGTCTCCATCAGCATCAGGCAAAGCCTCTCCATCTCATGATACTTCAAAGCGTTCATCACCAGCAGATAAAGCCTCTCCATCTTCAAAACCTTCATCACCAGCAGGCAAAGCCTCTCCATTTCAAAATACTTCAAAGCCTTCATCACTAGCAGGCAAAGCCTCCCCATCTAAGGATGCTTCAAAGCCTTCATCACCAGCAGGCAAGGCCTCTCCATCTCAGGATGCTTCAAAGCCTTCATCACCAGCTGCTCCTCGATCCCGAATGGCTTCGAAGCCACCGTCTCCATCTCAAACATCCAGTAAAAACCATCCAAATTCAAAACCAACATCACAATCGAGACTGAAAGCTGATTCTCAACCTCAAGTTTCTTCAATGCCGCGGTCACCATCTCGAAAACAACCATCGAAAAAAACCTCCCGAGTTCAGTCTCCATCTCATTTGTCCAGTAAACCTATTGCACAATCAACATCACCACAGCATATTGAATCTCCTGCAGCCATTGGAGACCAAACAACAAAAGAAATTGTTTCTCATCCAGCAAATCAATCGCCACAAGCAAGACCTAAAAGCAGGGAAAGTCAGTTGGAAACGAAATCAAAGCAGTCTCCAAAACCTGACTCGAAACCAGTGGAGTCCAGAGCATCAAAAGATCAGCCTGAAACCAATGAAGAGCCCACACTCACATCTAAGAACACTTCCCATCCCCATTCAAACCAGGACTCTTCTGAAATCCCAATACAGCCCAATCAAACCATTGAAAATGGTCTAGAGCCCTCTCTAGAATCACAGGAAGAGTCAAAGGAAAATAAGGAAGATCAGGCAAAAACAATCAATGCACTTCAAACCAGTGCATCAAGAAGCACATTGATCACATCTTCCGAAAGCCCTTCACCATTTGAACAAGAAGATAAGGACTCAAAACAGGAAGAAACCATGGAAGACTTATCAAAAGCTTTTCAGAAACTAAACATCAAATACTCAGACGAAGAAAATCCAAAGAGTTTCACAACACTCATCGGCGATAACAAAGGGGCTTCAATGCACGTACATTCCGGTGAAGCCAAGAGAGAGAGTTCAATCCACATCCACCGTGATTACAAGAGCGATCCAGACCAAAGCCCTGAAAGTGAAAGTTCCACAGACGACGAAGGAAACTTCAATAACGAAACACCACAAGAATCAAGAACAGAAGAGGATCCACCACTGGAGTTATACATAAACATCAACGTACAAGGTATCAACAACTCAATCCTGTCCAATACCTCATTCACTGAGAATAATCCTGGAATCAAGTTGAAATTCGTTCGAGAACCAACTAGATCTGAAGATGAATTACAGTCTCATGACGCTCGAGAGGCCAAGTATACTGCGAAACCTGCCGAGAGGCTTACATATGAGCCCACAGTAAGAAGAAGATGCCTCAGAGGGCTGTTAATGGAGTCGAGCGATTCTGAGCTCGACGATCCAGAAAAGCCCCGACGCCATGGCTGCCGCTACAGTCGAGGTTGCGAAGGAAAAGAGGTCGAAATTCTGTAG

Protein sequence

MSYRQFRFRLPWQSVKASSHPADESSRRSSEPTDETKASSSVADTVPNIWHQPGQSPEIKPEQPPLAPAQAAEKSETMPPSKSHKEAKVHAQSPSHSRAKNQTRTASKPPSPSKAIPQSSVASNKSPSASGKASPSHDTSKRSSPADKASPSSKPSSPAGKASPFQNTSKPSSLAGKASPSKDASKPSSPAGKASPSQDASKPSSPAAPRSRMASKPPSPSQTSSKNHPNSKPTSQSRLKADSQPQVSSMPRSPSRKQPSKKTSRVQSPSHLSSKPIAQSTSPQHIESPAAIGDQTTKEIVSHPANQSPQARPKSRESQLETKSKQSPKPDSKPVESRASKDQPETNEEPTLTSKNTSHPHSNQDSSEIPIQPNQTIENGLEPSLESQEESKENKEDQAKTINALQTSASRSTLITSSESPSPFEQEDKDSKQEETMEDLSKAFQKLNIKYSDEENPKSFTTLIGDNKGASMHVHSGEAKRESSIHIHRDYKSDPDQSPESESSTDDEGNFNNETPQESRTEEDPPLELYININVQGINNSILSNTSFTENNPGIKLKFVREPTRSEDELQSHDAREAKYTAKPAERLTYEPTVRRRCLRGLLMESSDSELDDPEKPRRHGCRYSRGCEGKEVEIL
Homology
BLAST of Lag0004395 vs. NCBI nr
Match: XP_023002262.1 (cell wall protein RBR3-like [Cucurbita maxima])

HSP 1 Score: 712.6 bits (1838), Expect = 3.0e-201
Identity = 457/656 (69.66%), Postives = 510/656 (77.74%), Query Frame = 0

Query: 1   MSYRQFRFRLPWQSVKASSHPADESSRRSSEPTDETKASSSVADTVPNIWHQPGQSPEIK 60
           M+YRQFRFRLPWQS+KASS P +ESS RSSEPTDE + S+S ADTVP + H P QS E K
Sbjct: 1   MTYRQFRFRLPWQSIKASSRPENESSTRSSEPTDEAETSNSAADTVPYMQHLPLQSHETK 60

Query: 61  PEQPPLAPAQAAEKSETMPPSKSHKEAKVHAQSPSHSRAKNQTRTASKPPSPSKAIPQSS 120
           PE  PL  AQA E+SETM PSKSHK+AKVH+Q  SHSRAK QTRTA+KPPS SK  PQSS
Sbjct: 61  PELSPLESAQAPERSETMLPSKSHKKAKVHSQPSSHSRAKKQTRTATKPPSASKVTPQSS 120

Query: 121 VASNKSPSASGKASPSHDTSKRSSPADKASPS---SKPSSPAGKASPFQNTSKPSSLAGK 180
           V+SNKSP+ S KASPSHD SK SS A K SPS   SK SSPAGK              GK
Sbjct: 121 VSSNKSPTTSAKASPSHDASKPSSSAGKVSPSHDTSKLSSPAGK--------------GK 180

Query: 181 ASPSKDASKPSSPAGKASPSQDASKPSS--PAAPRSRMASKPPSPSQTSSKNHPNSKPTS 240
            SPS+D S PS PAGKA PS+DAS+PSS   AAPRS + SKPPSPSQTSSKNH +SK TS
Sbjct: 181 VSPSRDTSMPSPPAGKAFPSRDASQPSSAAAAAPRSHIRSKPPSPSQTSSKNHLHSKQTS 240

Query: 241 QSRLKADSQ----------PQVSSMPRSP----SRKQPSKKTSRVQSPSHLSSKPIAQST 300
           QSRLKADSQ          PQ SS+PRSP    SR+QPSKK SRVQSPSHLSSK  AQST
Sbjct: 241 QSRLKADSQPSSPSRPAFSPQASSIPRSPSHENSRQQPSKKASRVQSPSHLSSKATAQST 300

Query: 301 SPQHIESPAAIGDQTTKEIVSHPANQSPQARPKSRESQLETKSKQSPKPDSKPVESRASK 360
           S Q  ESPA IGDQTTK +VSHPA+QSP+AR KS+E+Q++TKSKQSPKPD KPVE +ASK
Sbjct: 301 SQQLTESPATIGDQTTKRVVSHPADQSPRARCKSKENQVQTKSKQSPKPDLKPVEFKASK 360

Query: 361 DQPETNEEPTLTSKNTSHPHSNQDSSEIPIQPNQTIENGLEPSLESQEESKENK------ 420
            QPET EE    SKNTS+P SN+D SEIPI  ++TIENG EPSLESQ ES+E+K      
Sbjct: 361 HQPETMEE--FISKNTSYPLSNEDFSEIPIIIDETIENGPEPSLESQTESQESKEIKSYE 420

Query: 421 EDQAKTINALQTSASRSTLITSSESPSPFEQEDKDSKQEETMEDLSKAFQKLNIKYSDEE 480
           ED  KT NALQ +AS+S LITS+E  SPFE E+ DS+QE TMEDL KAFQ LNIKY  EE
Sbjct: 421 EDLEKTTNALQINASKSKLITSAEITSPFEPENSDSQQEGTMEDLPKAFQTLNIKY-PEE 480

Query: 481 NPKSFTTLIGDNKGASMHVHSGEAKRESSIHIHRDYKSDPDQSPESESSTDDEGNFNNET 540
           NPKSFTTL GDNKGASMH+ SGEA +ESSIHIHR YKSDPD+ P  ESSTD EGN N ET
Sbjct: 481 NPKSFTTLTGDNKGASMHLISGEATKESSIHIHRQYKSDPDKVP--ESSTDIEGNSNEET 540

Query: 541 PQESRTEEDPPLELYININVQGINNSILSNTSFTENNPGIKLKFVREPTRSEDELQSHDA 600
           PQ+S+TEEDPPLELYININVQGINNS+LSN+SFTENNPGIKLKFV + T+SE++  S  A
Sbjct: 541 PQDSKTEEDPPLELYININVQGINNSLLSNSSFTENNPGIKLKFVPQQTKSENKPHSLQA 600

Query: 601 REAKYTAKPAERLTYEPTVRRRCLRGLLMESSDSELDDPEKPRRHGCRYSRGCEGK 632
           ++AKYTAK  E  TYEPTVRRRCL GLLMESSDS+ D+ EKPRRHGCRY    EGK
Sbjct: 601 QKAKYTAKHTENHTYEPTVRRRCLGGLLMESSDSDGDNSEKPRRHGCRYRGSFEGK 637

BLAST of Lag0004395 vs. NCBI nr
Match: XP_022951875.1 (cell wall protein RBR3-like [Cucurbita moschata])

HSP 1 Score: 711.4 bits (1835), Expect = 6.7e-201
Identity = 457/655 (69.77%), Postives = 507/655 (77.40%), Query Frame = 0

Query: 1   MSYRQFRFRLPWQSVKASSHPADESSRRSSEPTDETKASSSVADTVPNIWHQPGQSPEIK 60
           M+YRQFRFRLPWQS+KASS   +ESS RSSEPTDE + S+S ADTVP + H         
Sbjct: 1   MTYRQFRFRLPWQSIKASSRLENESSTRSSEPTDEAETSNSAADTVPYMQH--------L 60

Query: 61  PEQPPLAPAQAAEKSETMPPSKSHKEAKVHAQSPSHSRAKNQTRTASKPPSPSKAIPQSS 120
           PE  PL  AQA E+SETM PSKSHK+AKVH+Q  SHSRAK QTRTA+KPPS SK  PQSS
Sbjct: 61  PELSPLESAQAPERSETMLPSKSHKKAKVHSQPSSHSRAKKQTRTATKPPSASKVTPQSS 120

Query: 121 VASNKSPSASGKASPSHDTSKRSSPADKASPS---SKPSSPAGKASPFQNTSKPSSLAGK 180
           V+SNKSP+ S KASPSHD SK SS A K SPS   SK SSPAGK              GK
Sbjct: 121 VSSNKSPTTSAKASPSHDASKPSSSAGKVSPSHDTSKLSSPAGK--------------GK 180

Query: 181 ASPSKDASKPSSPAGKASPSQDASKPSS--PAAPRSRMASKPPSPSQTSSKNHPNSKPTS 240
            SPS D SKPSSPAGKA PS+DAS+PSS   AAPRS++ SKPPSPSQTSSKNHP SKPTS
Sbjct: 181 VSPSHDTSKPSSPAGKAFPSRDASQPSSSAAAAPRSQIRSKPPSPSQTSSKNHPQSKPTS 240

Query: 241 QSRLKADSQ----------PQVSSMPRSP----SRKQPSKKTSRVQSPSHLSSKPIAQST 300
           QSRLKADSQ          PQ SS+PRSP    SR+QPSKK SRVQSPSHLSSKP AQST
Sbjct: 241 QSRLKADSQPSSPSRPAFSPQASSIPRSPSHENSRQQPSKKASRVQSPSHLSSKPTAQST 300

Query: 301 SPQHIESPAAIGDQTTKEIVSHPANQSPQARPKSRESQLETKSKQSPKPDSKPVESRASK 360
           S Q  ESPA IGDQTTK +VSHPA+QSP+AR K RE+Q++TKSKQSPKPD KPVE +ASK
Sbjct: 301 SQQLTESPATIGDQTTKRVVSHPADQSPRARCKRRENQVQTKSKQSPKPDLKPVEFKASK 360

Query: 361 DQPETNEEPTLTSKNTSHPHSNQDSSEIPIQPNQTIENGLEPSLESQEESKENK------ 420
            QPET EE    SKNTS+PHS+QD SEIPI  ++TIENG E SLESQ ES+E+K      
Sbjct: 361 HQPETMEE--FISKNTSYPHSDQDFSEIPIIIDETIENGPETSLESQTESQESKEIKSYE 420

Query: 421 EDQAKTINALQTSASRSTLITSSESPSPFEQEDKDSKQEETMEDLSKAFQKLNIKYSDEE 480
           ED  KT NALQ +AS+S LITS+E  SPFE E+ DS+QE TMEDLSKAFQ LNIKY  EE
Sbjct: 421 EDLEKTTNALQINASKSKLITSAEITSPFEPENSDSQQEGTMEDLSKAFQTLNIKY-PEE 480

Query: 481 NPKSFTTLIGDNKGASMHVHSGEAKRESSIHIHRDYKSDPDQSPESESSTDDEGNFNNET 540
           NPKSFTTL GDNKGASMH+ SGEA +ESSIHIHR YKSDPD+ P  ESSTD EGN N ET
Sbjct: 481 NPKSFTTLTGDNKGASMHLLSGEATKESSIHIHRQYKSDPDKGP--ESSTDIEGNSNEET 540

Query: 541 PQESRTEEDPPLELYININVQGINNSILSNTSFTENNPGIKLKFVREPTRSEDELQSHDA 600
           PQ+S+TEEDPPLELYININVQGINNS+LSN+SFTENNPGIKLKFV + T+SED+  S  A
Sbjct: 541 PQDSKTEEDPPLELYININVQGINNSLLSNSSFTENNPGIKLKFVPQQTKSEDKSHSLQA 600

Query: 601 REAKYTAKPAERLTYEPTVRRRCLRGLLMESSDSELDDPEKPRRHGCRYSRGCEG 631
           ++AKYTAK  E  TYEPTVRRRCL GLLMESSDS+ D+ EKPRRHGCRY    EG
Sbjct: 601 QKAKYTAKHTENHTYEPTVRRRCLGGLLMESSDSDGDNSEKPRRHGCRYRGSFEG 628

BLAST of Lag0004395 vs. NCBI nr
Match: XP_023537866.1 (cell wall protein RBR3-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 707.2 bits (1824), Expect = 1.3e-199
Identity = 457/656 (69.66%), Postives = 505/656 (76.98%), Query Frame = 0

Query: 1   MSYRQFRFRLPWQSVKASSHPADESSRRSSEPTDETKASSSVADTVPNIWHQPGQSPEIK 60
           M+ RQFRFRLPWQSVKASS P +ESS RSSEPTDE + S+S ADTVP + H         
Sbjct: 1   MTNRQFRFRLPWQSVKASSRPENESSTRSSEPTDEAETSNSAADTVPYMQH--------L 60

Query: 61  PEQPPLAPAQAAEKSETMPPSKSHKEAKVHAQSPSHSRAKNQTRTASKPPSPSKAIPQSS 120
           PE  PL  AQA E SETM PSKSHK+AKVH+Q  SHSRAK QTRTA+KPPS SK  PQSS
Sbjct: 61  PELSPLESAQAPETSETMLPSKSHKKAKVHSQPSSHSRAKKQTRTATKPPSASKVTPQSS 120

Query: 121 VASNKSPSASGKASPSHDTSKRSSPADKASPS---SKPSSPAGKASPFQNTSKPSSLAGK 180
           V+SNKSP+ S KASPSHD SK SS A K SPS   SK SSPAGK              GK
Sbjct: 121 VSSNKSPTTSAKASPSHDASKPSSSAGKVSPSHDTSKLSSPAGK--------------GK 180

Query: 181 ASPSKDASKPSSPAGKASPSQDASKPSS--PAAPRSRMASKPPSPSQTSSKNHPNSKPTS 240
            SPS D SKPSSPAGKA PS+DAS+PSS   AAPRS++ SKPPSPSQTSSKNHP SKPTS
Sbjct: 181 VSPSHDTSKPSSPAGKAFPSRDASQPSSSAAAAPRSQIRSKPPSPSQTSSKNHPQSKPTS 240

Query: 241 QSRLKADSQ----------PQVSSMPRSP----SRKQPSKKTSRVQSPSHLSSKPIAQST 300
           QSRLKADSQ          PQ SS+PRSP    SR+QPSKK SRVQSPSHLSSKP AQST
Sbjct: 241 QSRLKADSQPSSPSRPALSPQASSIPRSPSHENSRQQPSKKASRVQSPSHLSSKPTAQST 300

Query: 301 SPQHIESPAAIGDQTTKEIVSHPANQSPQARPKSRESQLETKSKQSPKPDSKPVESRASK 360
           S Q  ESPA IGDQTTK +VSHPA+QSP+AR KSRE+Q++TKSKQSPKPD KPVE +ASK
Sbjct: 301 SQQLTESPATIGDQTTKRVVSHPADQSPRARCKSRENQVQTKSKQSPKPDLKPVEFKASK 360

Query: 361 DQPETNEEPTLTSKNTSHPHSNQDSSEIPIQPNQTIENGLEPSLESQEESKENK------ 420
            QPET EE    SKNTS+PHS+QD SEIPI  ++TIENG E SLESQ ES+E+K      
Sbjct: 361 HQPETMEE--FISKNTSYPHSDQDFSEIPIIIDETIENGPETSLESQTESQESKEIKSYE 420

Query: 421 EDQAKTINALQTSASRSTLITSSESPSPFEQEDKDSKQEETMEDLSKAFQKLNIKYSDEE 480
           ED  KT NALQ +AS+S LITS+E  SPFE E+ DS+QE TMEDLSKAFQ LNIKY  EE
Sbjct: 421 EDLEKTTNALQINASKSKLITSAEITSPFEPENSDSQQEGTMEDLSKAFQTLNIKY-PEE 480

Query: 481 NPKSFTTLIGDNKGASMHVHSGEAKRESSIHIHRDYKSDPDQSPESESSTDDEGNFNNET 540
           NPKSFTTL GDNKGASMH+ SGEA +ESSIHIHR YKSDPD+ P  ESSTD EGN N ET
Sbjct: 481 NPKSFTTLTGDNKGASMHLLSGEATKESSIHIHRQYKSDPDKGP--ESSTDIEGNSNEET 540

Query: 541 PQESRTEEDPPLELYININVQGINNSILSNTSFTENNPGIKLKFVREPTRSEDELQSHDA 600
           PQ+S+TEEDPPLELYININVQGINNS+LSN+SFTENNPGIKL FV + T+SED+  S  A
Sbjct: 541 PQDSKTEEDPPLELYININVQGINNSLLSNSSFTENNPGIKLNFVPQQTKSEDKPHSLQA 600

Query: 601 REAKYTAKPAERLTYEPTVRRRCLRGLLMESSDSELDDPEKPRRHGCRYSRGCEGK 632
           ++AKYTAK  E   YE TVRRRCL GLLMESSDS+ D+ EKPRRHGCRY    EGK
Sbjct: 601 QKAKYTAKHTENHIYESTVRRRCLGGLLMESSDSDGDNSEKPRRHGCRYRGSFEGK 629

BLAST of Lag0004395 vs. NCBI nr
Match: KAG7020502.1 (hypothetical protein SDJN02_17187, partial [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 705.3 bits (1819), Expect = 4.8e-199
Identity = 455/657 (69.25%), Postives = 505/657 (76.86%), Query Frame = 0

Query: 1   MSYRQFRFRLPWQSVKASSHPADESSRRSSEPTDETKASSSVADTVPNIWHQPGQSPEIK 60
           M+YRQFRFRLPWQS+KASS   +ESS RSSEPTDE + S+S ADTVP + H         
Sbjct: 1   MTYRQFRFRLPWQSIKASSRLENESSTRSSEPTDEAETSNSAADTVPYMQH--------P 60

Query: 61  PEQPPLAPAQAAEKSETMPPSKSHKEAKVHAQSPSHSRAKNQTRTASKPPSPSKAIPQSS 120
           PE  PL  AQA E+SETM PSKSHK+AKVH+Q  SHSRAK QTRTA+KPPS SK  PQSS
Sbjct: 61  PELSPLESAQAPERSETMLPSKSHKKAKVHSQPSSHSRAKKQTRTATKPPSASKVTPQSS 120

Query: 121 VASNKSPSASGKASPSHDTSKRSSPADKASPS---SKPSSPAGKASPFQNTSKPSSLAGK 180
           V+SNKSP+ S KASPSHD SK SS A K SPS   SK SSPAGK              GK
Sbjct: 121 VSSNKSPTTSAKASPSHDASKPSSSAGKVSPSHDTSKLSSPAGK--------------GK 180

Query: 181 ASPSKDASKPSSPAGKASPSQDASKPSS--PAAPRSRMASKPPSPSQTSSKNHPNSKPTS 240
            SPS D SKPSSPAGKA PS+DAS+PSS   AAPRS++ SKPPSPSQTSSKNHP SKPTS
Sbjct: 181 VSPSHDTSKPSSPAGKAFPSRDASQPSSSAAAAPRSQIRSKPPSPSQTSSKNHPQSKPTS 240

Query: 241 QSRLKADS-----------QPQVSSMPRSP----SRKQPSKKTSRVQSPSHLSSKPIAQS 300
           QSRLKADS            PQ SS+PRSP    SR+QPSKK S VQSPSHLSSKP AQS
Sbjct: 241 QSRLKADSPQSSSPSRPAFSPQASSIPRSPSHENSRQQPSKKASLVQSPSHLSSKPTAQS 300

Query: 301 TSPQHIESPAAIGDQTTKEIVSHPANQSPQARPKSRESQLETKSKQSPKPDSKPVESRAS 360
           TS Q  ESPA IGDQTTK +VSHPA+QSP AR K RE+Q++TKSKQSPKPD KPVE +AS
Sbjct: 301 TSQQLTESPATIGDQTTKRVVSHPADQSPGARCKIRENQVQTKSKQSPKPDLKPVEFKAS 360

Query: 361 KDQPETNEEPTLTSKNTSHPHSNQDSSEIPIQPNQTIENGLEPSLESQEESKENK----- 420
           K QPET EE    SKNTS+PHS+QD SEIPI  ++T ENG EPSLESQ ES+E+K     
Sbjct: 361 KHQPETMEE--FISKNTSYPHSDQDFSEIPIIIDETNENGPEPSLESQTESQESKEIKSY 420

Query: 421 -EDQAKTINALQTSASRSTLITSSESPSPFEQEDKDSKQEETMEDLSKAFQKLNIKYSDE 480
            ED  KT NALQ +AS+S LITS+E  SPFE E+ DS+QE TMEDLSKAFQ LNIKY  E
Sbjct: 421 EEDLEKTTNALQINASKSKLITSAEITSPFEPENSDSQQEGTMEDLSKAFQTLNIKY-PE 480

Query: 481 ENPKSFTTLIGDNKGASMHVHSGEAKRESSIHIHRDYKSDPDQSPESESSTDDEGNFNNE 540
           ENPKSFTTL GDNKGASMH+ SGEA +ES+IHIHR YKSDPD+ P  ESSTD EGN N E
Sbjct: 481 ENPKSFTTLTGDNKGASMHLLSGEATKESAIHIHRQYKSDPDKVP--ESSTDIEGNSNEE 540

Query: 541 TPQESRTEEDPPLELYININVQGINNSILSNTSFTENNPGIKLKFVREPTRSEDELQSHD 600
           TPQ+S+TEEDPPLELYININVQGINNS+LSN+SFTENNPGIKLKFV + T+SED+  S  
Sbjct: 541 TPQDSKTEEDPPLELYININVQGINNSLLSNSSFTENNPGIKLKFVPQQTKSEDKPHSLH 600

Query: 601 AREAKYTAKPAERLTYEPTVRRRCLRGLLMESSDSELDDPEKPRRHGCRYSRGCEGK 632
           A++AKYTAK  E  TYEPTVRRRCL GLLMESSDS+ D+ EKPRRHGCRY    EGK
Sbjct: 601 AQKAKYTAKHTENHTYEPTVRRRCLAGLLMESSDSDGDNSEKPRRHGCRYRGSFEGK 630

BLAST of Lag0004395 vs. NCBI nr
Match: KAG6585587.1 (hypothetical protein SDJN03_18320, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 703.7 bits (1815), Expect = 1.4e-198
Identity = 454/657 (69.10%), Postives = 505/657 (76.86%), Query Frame = 0

Query: 1   MSYRQFRFRLPWQSVKASSHPADESSRRSSEPTDETKASSSVADTVPNIWHQPGQSPEIK 60
           M+YRQFRFRLPWQS+KASS   +ESS RSSEPTDE + S+S ADTVP + H         
Sbjct: 1   MTYRQFRFRLPWQSIKASSRLENESSTRSSEPTDEAETSNSAADTVPYMQH--------P 60

Query: 61  PEQPPLAPAQAAEKSETMPPSKSHKEAKVHAQSPSHSRAKNQTRTASKPPSPSKAIPQSS 120
           PE  PL  AQA E+SETM PSKSHK+AKVH+Q  SHSRAK QTRTA+KPPS SK  PQSS
Sbjct: 61  PELSPLESAQAPERSETMLPSKSHKKAKVHSQPSSHSRAKKQTRTATKPPSASKVTPQSS 120

Query: 121 VASNKSPSASGKASPSHDTSKRSSPADKASPS---SKPSSPAGKASPFQNTSKPSSLAGK 180
           V+SNKSP+ S KASPSHD SK SS A K SPS   SK SSPAGK              GK
Sbjct: 121 VSSNKSPTTSAKASPSHDASKPSSSAGKVSPSHDTSKLSSPAGK--------------GK 180

Query: 181 ASPSKDASKPSSPAGKASPSQDASKPSS--PAAPRSRMASKPPSPSQTSSKNHPNSKPTS 240
            SPS D SKPSSPAGKA PS+DAS+PSS   AAPRS++ SKPPSPSQTSSKNHP SKPTS
Sbjct: 181 VSPSHDTSKPSSPAGKAFPSRDASQPSSSAAAAPRSQIRSKPPSPSQTSSKNHPQSKPTS 240

Query: 241 QSRLKADS-----------QPQVSSMPRSP----SRKQPSKKTSRVQSPSHLSSKPIAQS 300
           QSRLKADS            PQ SS+PRSP    SR+QPSKK SRVQSPSHLSSKP AQS
Sbjct: 241 QSRLKADSPQPSSPSWPAFSPQASSIPRSPSHENSRQQPSKKASRVQSPSHLSSKPTAQS 300

Query: 301 TSPQHIESPAAIGDQTTKEIVSHPANQSPQARPKSRESQLETKSKQSPKPDSKPVESRAS 360
           TS Q  ESPA I DQTTK +VSHPA+QSP AR K +E+Q++TKSKQSPKPD KPVE +AS
Sbjct: 301 TSQQLTESPATIEDQTTKRVVSHPADQSPGARCKIKENQVQTKSKQSPKPDLKPVEFKAS 360

Query: 361 KDQPETNEEPTLTSKNTSHPHSNQDSSEIPIQPNQTIENGLEPSLESQEESKENK----- 420
           K QPET EE    SKNTS+PHS+QD SEIPI  ++T ENG EPSLESQ ES+E+K     
Sbjct: 361 KHQPETMEE--FISKNTSYPHSDQDFSEIPIIIDETNENGPEPSLESQTESQESKEIKSY 420

Query: 421 -EDQAKTINALQTSASRSTLITSSESPSPFEQEDKDSKQEETMEDLSKAFQKLNIKYSDE 480
            ED  KT NALQ +AS+S LITS+E  SPFE E+ DS+QE TMEDLSKAFQ LNIKY  E
Sbjct: 421 EEDLEKTTNALQINASKSKLITSAEITSPFEPENSDSQQEGTMEDLSKAFQTLNIKY-PE 480

Query: 481 ENPKSFTTLIGDNKGASMHVHSGEAKRESSIHIHRDYKSDPDQSPESESSTDDEGNFNNE 540
           ENPKSFTTL GDNKGASMH+ SGEA +ES+IHIHR YKSDPD+ P  ESSTD EGN N E
Sbjct: 481 ENPKSFTTLTGDNKGASMHLLSGEATKESAIHIHRQYKSDPDKVP--ESSTDIEGNSNEE 540

Query: 541 TPQESRTEEDPPLELYININVQGINNSILSNTSFTENNPGIKLKFVREPTRSEDELQSHD 600
           TPQ+S+TEEDPPLELYININVQGINNS+LSN+SFTENNPGIKLKFV + T+SED+  S  
Sbjct: 541 TPQDSKTEEDPPLELYININVQGINNSLLSNSSFTENNPGIKLKFVPQQTKSEDKPHSLH 600

Query: 601 AREAKYTAKPAERLTYEPTVRRRCLRGLLMESSDSELDDPEKPRRHGCRYSRGCEGK 632
           A++AKYTAK  E  TYEPTVRRRCL GLLMESSDS+ D+ EKPRRHGCRY    EGK
Sbjct: 601 AQKAKYTAKHTENHTYEPTVRRRCLAGLLMESSDSDGDNSEKPRRHGCRYRGSFEGK 630

BLAST of Lag0004395 vs. ExPASy TrEMBL
Match: A0A6J1KJ10 (cell wall protein RBR3-like OS=Cucurbita maxima OX=3661 GN=LOC111496164 PE=4 SV=1)

HSP 1 Score: 712.6 bits (1838), Expect = 1.5e-201
Identity = 457/656 (69.66%), Postives = 510/656 (77.74%), Query Frame = 0

Query: 1   MSYRQFRFRLPWQSVKASSHPADESSRRSSEPTDETKASSSVADTVPNIWHQPGQSPEIK 60
           M+YRQFRFRLPWQS+KASS P +ESS RSSEPTDE + S+S ADTVP + H P QS E K
Sbjct: 1   MTYRQFRFRLPWQSIKASSRPENESSTRSSEPTDEAETSNSAADTVPYMQHLPLQSHETK 60

Query: 61  PEQPPLAPAQAAEKSETMPPSKSHKEAKVHAQSPSHSRAKNQTRTASKPPSPSKAIPQSS 120
           PE  PL  AQA E+SETM PSKSHK+AKVH+Q  SHSRAK QTRTA+KPPS SK  PQSS
Sbjct: 61  PELSPLESAQAPERSETMLPSKSHKKAKVHSQPSSHSRAKKQTRTATKPPSASKVTPQSS 120

Query: 121 VASNKSPSASGKASPSHDTSKRSSPADKASPS---SKPSSPAGKASPFQNTSKPSSLAGK 180
           V+SNKSP+ S KASPSHD SK SS A K SPS   SK SSPAGK              GK
Sbjct: 121 VSSNKSPTTSAKASPSHDASKPSSSAGKVSPSHDTSKLSSPAGK--------------GK 180

Query: 181 ASPSKDASKPSSPAGKASPSQDASKPSS--PAAPRSRMASKPPSPSQTSSKNHPNSKPTS 240
            SPS+D S PS PAGKA PS+DAS+PSS   AAPRS + SKPPSPSQTSSKNH +SK TS
Sbjct: 181 VSPSRDTSMPSPPAGKAFPSRDASQPSSAAAAAPRSHIRSKPPSPSQTSSKNHLHSKQTS 240

Query: 241 QSRLKADSQ----------PQVSSMPRSP----SRKQPSKKTSRVQSPSHLSSKPIAQST 300
           QSRLKADSQ          PQ SS+PRSP    SR+QPSKK SRVQSPSHLSSK  AQST
Sbjct: 241 QSRLKADSQPSSPSRPAFSPQASSIPRSPSHENSRQQPSKKASRVQSPSHLSSKATAQST 300

Query: 301 SPQHIESPAAIGDQTTKEIVSHPANQSPQARPKSRESQLETKSKQSPKPDSKPVESRASK 360
           S Q  ESPA IGDQTTK +VSHPA+QSP+AR KS+E+Q++TKSKQSPKPD KPVE +ASK
Sbjct: 301 SQQLTESPATIGDQTTKRVVSHPADQSPRARCKSKENQVQTKSKQSPKPDLKPVEFKASK 360

Query: 361 DQPETNEEPTLTSKNTSHPHSNQDSSEIPIQPNQTIENGLEPSLESQEESKENK------ 420
            QPET EE    SKNTS+P SN+D SEIPI  ++TIENG EPSLESQ ES+E+K      
Sbjct: 361 HQPETMEE--FISKNTSYPLSNEDFSEIPIIIDETIENGPEPSLESQTESQESKEIKSYE 420

Query: 421 EDQAKTINALQTSASRSTLITSSESPSPFEQEDKDSKQEETMEDLSKAFQKLNIKYSDEE 480
           ED  KT NALQ +AS+S LITS+E  SPFE E+ DS+QE TMEDL KAFQ LNIKY  EE
Sbjct: 421 EDLEKTTNALQINASKSKLITSAEITSPFEPENSDSQQEGTMEDLPKAFQTLNIKY-PEE 480

Query: 481 NPKSFTTLIGDNKGASMHVHSGEAKRESSIHIHRDYKSDPDQSPESESSTDDEGNFNNET 540
           NPKSFTTL GDNKGASMH+ SGEA +ESSIHIHR YKSDPD+ P  ESSTD EGN N ET
Sbjct: 481 NPKSFTTLTGDNKGASMHLISGEATKESSIHIHRQYKSDPDKVP--ESSTDIEGNSNEET 540

Query: 541 PQESRTEEDPPLELYININVQGINNSILSNTSFTENNPGIKLKFVREPTRSEDELQSHDA 600
           PQ+S+TEEDPPLELYININVQGINNS+LSN+SFTENNPGIKLKFV + T+SE++  S  A
Sbjct: 541 PQDSKTEEDPPLELYININVQGINNSLLSNSSFTENNPGIKLKFVPQQTKSENKPHSLQA 600

Query: 601 REAKYTAKPAERLTYEPTVRRRCLRGLLMESSDSELDDPEKPRRHGCRYSRGCEGK 632
           ++AKYTAK  E  TYEPTVRRRCL GLLMESSDS+ D+ EKPRRHGCRY    EGK
Sbjct: 601 QKAKYTAKHTENHTYEPTVRRRCLGGLLMESSDSDGDNSEKPRRHGCRYRGSFEGK 637

BLAST of Lag0004395 vs. ExPASy TrEMBL
Match: A0A6J1GK50 (cell wall protein RBR3-like OS=Cucurbita moschata OX=3662 GN=LOC111454611 PE=4 SV=1)

HSP 1 Score: 711.4 bits (1835), Expect = 3.3e-201
Identity = 457/655 (69.77%), Postives = 507/655 (77.40%), Query Frame = 0

Query: 1   MSYRQFRFRLPWQSVKASSHPADESSRRSSEPTDETKASSSVADTVPNIWHQPGQSPEIK 60
           M+YRQFRFRLPWQS+KASS   +ESS RSSEPTDE + S+S ADTVP + H         
Sbjct: 1   MTYRQFRFRLPWQSIKASSRLENESSTRSSEPTDEAETSNSAADTVPYMQH--------L 60

Query: 61  PEQPPLAPAQAAEKSETMPPSKSHKEAKVHAQSPSHSRAKNQTRTASKPPSPSKAIPQSS 120
           PE  PL  AQA E+SETM PSKSHK+AKVH+Q  SHSRAK QTRTA+KPPS SK  PQSS
Sbjct: 61  PELSPLESAQAPERSETMLPSKSHKKAKVHSQPSSHSRAKKQTRTATKPPSASKVTPQSS 120

Query: 121 VASNKSPSASGKASPSHDTSKRSSPADKASPS---SKPSSPAGKASPFQNTSKPSSLAGK 180
           V+SNKSP+ S KASPSHD SK SS A K SPS   SK SSPAGK              GK
Sbjct: 121 VSSNKSPTTSAKASPSHDASKPSSSAGKVSPSHDTSKLSSPAGK--------------GK 180

Query: 181 ASPSKDASKPSSPAGKASPSQDASKPSS--PAAPRSRMASKPPSPSQTSSKNHPNSKPTS 240
            SPS D SKPSSPAGKA PS+DAS+PSS   AAPRS++ SKPPSPSQTSSKNHP SKPTS
Sbjct: 181 VSPSHDTSKPSSPAGKAFPSRDASQPSSSAAAAPRSQIRSKPPSPSQTSSKNHPQSKPTS 240

Query: 241 QSRLKADSQ----------PQVSSMPRSP----SRKQPSKKTSRVQSPSHLSSKPIAQST 300
           QSRLKADSQ          PQ SS+PRSP    SR+QPSKK SRVQSPSHLSSKP AQST
Sbjct: 241 QSRLKADSQPSSPSRPAFSPQASSIPRSPSHENSRQQPSKKASRVQSPSHLSSKPTAQST 300

Query: 301 SPQHIESPAAIGDQTTKEIVSHPANQSPQARPKSRESQLETKSKQSPKPDSKPVESRASK 360
           S Q  ESPA IGDQTTK +VSHPA+QSP+AR K RE+Q++TKSKQSPKPD KPVE +ASK
Sbjct: 301 SQQLTESPATIGDQTTKRVVSHPADQSPRARCKRRENQVQTKSKQSPKPDLKPVEFKASK 360

Query: 361 DQPETNEEPTLTSKNTSHPHSNQDSSEIPIQPNQTIENGLEPSLESQEESKENK------ 420
            QPET EE    SKNTS+PHS+QD SEIPI  ++TIENG E SLESQ ES+E+K      
Sbjct: 361 HQPETMEE--FISKNTSYPHSDQDFSEIPIIIDETIENGPETSLESQTESQESKEIKSYE 420

Query: 421 EDQAKTINALQTSASRSTLITSSESPSPFEQEDKDSKQEETMEDLSKAFQKLNIKYSDEE 480
           ED  KT NALQ +AS+S LITS+E  SPFE E+ DS+QE TMEDLSKAFQ LNIKY  EE
Sbjct: 421 EDLEKTTNALQINASKSKLITSAEITSPFEPENSDSQQEGTMEDLSKAFQTLNIKY-PEE 480

Query: 481 NPKSFTTLIGDNKGASMHVHSGEAKRESSIHIHRDYKSDPDQSPESESSTDDEGNFNNET 540
           NPKSFTTL GDNKGASMH+ SGEA +ESSIHIHR YKSDPD+ P  ESSTD EGN N ET
Sbjct: 481 NPKSFTTLTGDNKGASMHLLSGEATKESSIHIHRQYKSDPDKGP--ESSTDIEGNSNEET 540

Query: 541 PQESRTEEDPPLELYININVQGINNSILSNTSFTENNPGIKLKFVREPTRSEDELQSHDA 600
           PQ+S+TEEDPPLELYININVQGINNS+LSN+SFTENNPGIKLKFV + T+SED+  S  A
Sbjct: 541 PQDSKTEEDPPLELYININVQGINNSLLSNSSFTENNPGIKLKFVPQQTKSEDKSHSLQA 600

Query: 601 REAKYTAKPAERLTYEPTVRRRCLRGLLMESSDSELDDPEKPRRHGCRYSRGCEG 631
           ++AKYTAK  E  TYEPTVRRRCL GLLMESSDS+ D+ EKPRRHGCRY    EG
Sbjct: 601 QKAKYTAKHTENHTYEPTVRRRCLGGLLMESSDSDGDNSEKPRRHGCRYRGSFEG 628

BLAST of Lag0004395 vs. ExPASy TrEMBL
Match: A0A5A7VAN0 (Flocculation protein FLO11 OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold82G005610 PE=4 SV=1)

HSP 1 Score: 569.3 bits (1466), Expect = 2.0e-158
Identity = 424/793 (53.47%), Postives = 503/793 (63.43%), Query Frame = 0

Query: 1   MSYRQFRFRLPWQSVKASSHPADESSRRSSEPTDETKASSSVADTVPNIWHQPGQSPEIK 60
           MS  Q R  LPWQS+KAS  PA+ES   S  PTDE+++S+S ADT PNI HQP QSPEIK
Sbjct: 1   MSLSQLRILLPWQSLKASPRPANESPEGSFGPTDESESSASQADTAPNIRHQPDQSPEIK 60

Query: 61  PEQPPLAPAQAAEKSETMPPSKSHKEAKVHAQSPSHSRAKNQTRTASKPPSPSKAIPQSS 120
           PE+PPLA AQAAE+SETMPPSKSHKE K+H+Q  ++SRAKN++RTASKP SP  AIPQS 
Sbjct: 61  PEEPPLATAQAAERSETMPPSKSHKEGKIHSQLSTNSRAKNRSRTASKPSSPLNAIPQSP 120

Query: 121 VASNKSPSASGKASPSHDTSKRSSPADKA-SP----SSKPSSPAGKASPFQN-TSKPSSL 180
           +ASNK PS SGK S S D+SK SSPA K  SP    SSKPSSPA  A+      SK SS 
Sbjct: 121 LASNKYPSTSGKGSKSQDSSKPSSPAGKVFSPSQDASSKPSSPATVAATAPRIASKASSS 180

Query: 181 AGKAS----PS---------KDASKPSSPAGKASPSQDASKP------------------ 240
           + +AS    PS         K  S+PSSP+  A PSQD S P                  
Sbjct: 181 SSQASNKKHPSSKPTSKLRFKADSQPSSPSRSAFPSQDPSMPPQSLSQEKSRQQASEKSS 240

Query: 241 ----------------------SSPA------------------------APRSRMA--- 300
                                  SPA                        +P SR     
Sbjct: 241 RVQSPSHFSRKPTTQSTSKQPVESPATIGIQSHPNSKAPSQSRFKTDSQPSPSSRSTFPS 300

Query: 301 ---SKPP--------------------SPSQTSSK--------------------NHPNS 360
              S PP                    SPS +S K                    +HPN 
Sbjct: 301 QDFSMPPRSPSHENSRQQPSDKTSGVQSPSHSSRKPTAQSTSKQPIESPATIGIQHHPNL 360

Query: 361 KPTSQSRLKADSQPQVSSM-----------PRSPSRKQ----PSKKTSRVQSPSHLSSKP 420
           KP+SQSR KA+S+P  SS            PRSPS++     PS+KTSRVQSPS+LS KP
Sbjct: 361 KPSSQSRFKAESRPSSSSKSKFPSQDSSMPPRSPSQENSLQPPSEKTSRVQSPSNLSRKP 420

Query: 421 IAQSTSPQHIESPAAIGDQTTKEIVSHPANQSPQARPKSRESQLETKSKQSPKPDSKPVE 480
            A STS Q IES A+IGDQTT  I+S PA  SP+A P S E Q++ KSK+SP+P+ KPVE
Sbjct: 421 TAPSTSQQPIESTASIGDQTTDGILSDPATPSPKAIPTSGEIQIQAKSKKSPEPNVKPVE 480

Query: 481 SRASKDQPETNEEPT--------LTSKNTSHPHSNQDSSEIPIQPNQTIENGLEPSLESQ 540
            +ASK+Q +T EE T        L SKNTS+PHS++DSSE P Q ++T+E GL+ SLESQ
Sbjct: 481 LKASKNQNDTKEELTSKNETKEELASKNTSNPHSDEDSSENPTQSDETVERGLDSSLESQ 540

Query: 541 EESKENKEDQAKTINALQTSASRSTLITSSESPSPFEQEDKDSKQEETMEDLSKAFQKLN 600
            ESKE KED  KT NALQ  ASRSTLITSS+S S FE E K+++Q+E+MEDLSKAF KLN
Sbjct: 541 TESKETKEDGGKTTNALQAKASRSTLITSSKSRSSFEPE-KNTQQDESMEDLSKAFNKLN 600

Query: 601 IKYSDEENPKSFTTLIGDNKGASMHVHSGEAKRESSIHIHRDYKSDPDQSPESESSTDDE 637
           IKYSDEENPKSFTT+IGDNKG+S+H+ SGEAK ESSIH++  YKS+PDQSP  +SST+ +
Sbjct: 601 IKYSDEENPKSFTTMIGDNKGSSVHLLSGEAKSESSIHVNHRYKSNPDQSP--KSSTNIK 660

BLAST of Lag0004395 vs. ExPASy TrEMBL
Match: A0A6J1CRH0 (cell wall protein RBR3 OS=Momordica charantia OX=3673 GN=LOC111013918 PE=4 SV=1)

HSP 1 Score: 472.6 bits (1215), Expect = 2.5e-129
Identity = 331/566 (58.48%), Postives = 392/566 (69.26%), Query Frame = 0

Query: 78  MPPSKSHKEAKVHAQSPSHSRAKNQTRTASKPPSPSKAIPQSSVASNKSPSASGKASPSH 137
           MPPS+S KE++VH+ SPS+SRAKNQ R ASK PS  KA P  +VASNKS           
Sbjct: 1   MPPSQSKKESQVHSHSPSYSRAKNQPRIASKSPSLLKASP--TVASNKS----------- 60

Query: 138 DTSKRSSPADKASPSSKPSSPAGKASPFQNTSKPSSLAGKASPSKDASKPSSPAGKASPS 197
                            PSSPA               +GKASPSKDASKPSSPA      
Sbjct: 61  -----------------PSSPA---------------SGKASPSKDASKPSSPA------ 120

Query: 198 QDASKPSSPAAPRSRMASKPPSPSQTSSKNHPNSKPTS-QSRLKADSQPQVSSMPRSPSR 257
                    AAPR R++S PPSPSQTSS+NH N KPTS QS+LKADSQP  SS  RS  R
Sbjct: 121 --------VAAPRPRISSVPPSPSQTSSRNHLNPKPTSKQSKLKADSQP--SSSSRSAFR 180

Query: 258 KQPS---KKTSRVQSPSHLSSKPIAQSTSPQHIESPAAIGDQTTKEIVSHPANQSPQARP 317
            Q S   +  S+  S    S K  AQSTS QH    AA  DQTT  + SH AN+S QARP
Sbjct: 181 PQASSTARSPSQANSKQQPSKKTTAQSTSLQHTAFSAATEDQTTNGVTSHSANKSSQARP 240

Query: 318 KSRESQLETKSKQSPKPDSKPVESRASKDQPETNEEPTLTSKNTSHPHSNQDSSEIPIQP 377
           K RESQ +TKSKQSPK       S+ASK+QP+  EE  LTSKNTS+P SNQ+SSE P + 
Sbjct: 241 KGRESQSQTKSKQSPK-------SKASKNQPQIKEE--LTSKNTSNPQSNQNSSENPTRS 300

Query: 378 NQTIENGLEPSLESQEESKENKEDQAKTINALQTSASRSTLITSSESPSPFEQ-EDKDSK 437
           +Q+IENG +PSLESQ ESKE++E + K  NA  T    STLI+SSES SP+E+ ED+DS+
Sbjct: 301 DQSIENGQDPSLESQAESKEDEETKEKATNASAT----STLISSSESSSPYEEPEDRDSQ 360

Query: 438 QEETM--EDLSKAFQKLNIKYSDEENPKSFTTLIGDNKGASMHVHSGEAKRESSIHIHRD 497
           ++E M   D+SKAF KL I YS EENPKSF TLIGDNKG SM++ SG+  RESSIHI R+
Sbjct: 361 KKELMGEGDISKAFPKLKINYSGEENPKSFITLIGDNKGTSMNLLSGKGNRESSIHIRRE 420

Query: 498 YKSDPDQSPESESSTDDEGNFNNETPQESRTEEDPPLELYININVQGINNSILSNTSFTE 557
           Y+S+PDQSP  +SST+ EGNFN++T ++SRT EDPPL LYIN N QGINNSILSN+SFTE
Sbjct: 421 YQSNPDQSP--KSSTEIEGNFNHKTTEDSRTVEDPPLRLYINNNTQGINNSILSNSSFTE 480

Query: 558 NNPGIKLKFVREPTRSEDELQSHDAREAKYTAKPAERLTYEPTVRRRCLRGLLMESSDSE 617
            NPG +LKF REPT+SE+  +S   ++AKY AKPAERLTY+PTVRRRCLRGL MESSDSE
Sbjct: 481 RNPGARLKFFREPTKSEEISESLSTQKAKYIAKPAERLTYKPTVRRRCLRGLFMESSDSE 489

Query: 618 LDDPEKPRRHGCRYSRGCEGKEVEIL 637
            ++PEKPRRHGCRYS  C+GK+ EI+
Sbjct: 541 PENPEKPRRHGCRYS-NCKGKDGEIM 489

BLAST of Lag0004395 vs. ExPASy TrEMBL
Match: A0A0A0LLH1 (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_2G361770 PE=4 SV=1)

HSP 1 Score: 432.6 bits (1111), Expect = 2.9e-117
Identity = 265/402 (65.92%), Postives = 309/402 (76.87%), Query Frame = 0

Query: 251 PRSPSRKQ----PSKKTSRVQSPSHLSSKPIAQSTSPQHIESPAAIGDQTTKEIVSHPAN 310
           PRSPS++     PS+KT RVQSPSHLS KP AQSTS Q IE  A+IGDQTT  I+S PAN
Sbjct: 3   PRSPSQENSLQPPSEKTFRVQSPSHLSRKPTAQSTSQQPIEPTASIGDQTTDRILSDPAN 62

Query: 311 QSPQARPKSRESQLETKSKQSPKPDSKPVESRASKDQPETNEEPT--------LTSKNTS 370
            SP+A P S ESQ++ +SK+SPKP+ KPVE   SK Q ET EE T        L SKNTS
Sbjct: 63  PSPKAIPTSGESQIQAESKKSPKPNVKPVELEESKTQHETKEELTSKNENKEELASKNTS 122

Query: 371 HPHSNQDSSEIPIQPNQTIENGLEPSLESQEESKENKEDQAKTINALQTSASRSTLITSS 430
           +PHS +DSSE P Q +Q IE GL+ SLESQ ESKE KED AKT NA QT ASRSTLITSS
Sbjct: 123 NPHSYKDSSENPTQSDQAIEKGLDSSLESQTESKETKEDGAKTTNAFQTKASRSTLITSS 182

Query: 431 ESPSPFEQEDKDSKQEETMEDLSKAFQKLNIKYSDEENPKSFTTLIGDNKGASMHVHSGE 490
           +S S FE E+ +++Q+E+MEDLSKAF KLNIKYSDEENPKS TT+IGDNKG SMH+ S E
Sbjct: 183 KSRSSFEPEN-NTQQDESMEDLSKAFNKLNIKYSDEENPKSLTTMIGDNKGTSMHLLSDE 242

Query: 491 AKRESSIHIHRDYKSDPDQSPESESSTDDEGNFNNETPQESRTEED---PPLELYININV 550
           AK ESSIH++  YKS+PDQSP  ESSTD + N NNET ++S TEE+   PPLELYIN+NV
Sbjct: 243 AKSESSIHVNHHYKSNPDQSP--ESSTDIKENSNNETAKDSTTEENPDPPPLELYINLNV 302

Query: 551 QGINNSILSNTSFTENNPGIKLKFVREPTRSEDELQS-HDAREAKYTAKPAERLTYEPTV 610
           QGINNSI  NTSFTENNPGIKLKF  EPT  +DEL+S H  R++KY A PAE++TY+P +
Sbjct: 303 QGINNSITFNTSFTENNPGIKLKFPGEPTNCQDELESDHHTRKSKYIATPAEKVTYDPRI 362

Query: 611 RRRCLRGLLMESSDSELDDPEKPRRHGCRYSRGCEGKEVEIL 637
           RRRCL GLLMESSDSE ++P K + HGCRYS   +GKEVE L
Sbjct: 363 RRRCLEGLLMESSDSEDENPGKLQPHGCRYSGSSKGKEVETL 401

BLAST of Lag0004395 vs. TAIR 10
Match: AT1G75260.1 (oxidoreductases, acting on NADH or NADPH )

HSP 1 Score: 97.4 bits (241), Expect = 4.3e-20
Identity = 145/515 (28.16%), Postives = 240/515 (46.60%), Query Frame = 0

Query: 134 SPSHDTSKRSSPADKASPSSKPS-SPAGKASPFQNTSKPSSLAGKASPSKDASKPSSPAG 193
           SPS  +S  SSP+   +P S+P   PAG A P ++ +KP     KASPS   S+ S+ A 
Sbjct: 15  SPSRISSGTSSPSPPPTPPSRPPFRPAGIAQPSKSETKP-----KASPSLSRSR-SNVAA 74

Query: 194 KASPSQDASKPSSPAAPRSRMASKPPSPSQTSSKNHPNSKPTSQSRLKADSQP----QVS 253
            A+ S  +  PS  AA  +R+A +    S + SK   + +   Q     +  P    +++
Sbjct: 75  LAASSSASQLPSLGAATPTRLAKQTNQQSGSPSKKLDSLRMEEQKVATKEKPPGETEKIA 134

Query: 254 SMPRSPSRKQPSKKTSRVQSPSHLSSKPIAQSTSPQHIESPAAIGDQTTKEIV------S 313
               SP +++P       +   HL  K              A +  Q  K+++       
Sbjct: 135 EENISPVKEKP---PIGARPEEHLEQKETEAVQEQGRNTEAARLVVQENKKVLPEGSGKK 194

Query: 314 HPANQSPQARPKSRESQLETKSKQSPKPDSKPVESRASKDQPETNEEPTL-TSKNTSHPH 373
             ANQ  Q   +  +  L+ + K       + +E+   + + +  E+  L  +K +    
Sbjct: 195 SAANQGQQKIEEIEKIALQERKKVLHDDGVQKLEADQGQQKSKETEKLALQETKRSLQAV 254

Query: 374 SNQDSSEIPIQPNQTIENGLEPSLESQEESKENKEDQAKTINALQTSASRSTLITSSESP 433
             +D++      +    +          E K   +++ +         ++  L ++  +P
Sbjct: 255 GREDATRSKTTRHMAAASETTRGPRDLPEKKTETQNRTEIPTDDNHQKTKGALTSNLGNP 314

Query: 434 SPFEQEDKDSKQEETMEDLSKAFQKLNI-KYSDEENPKSFTTLIGDNKGASMHVHSGEAK 493
               +E   S   +  ED+     KL   K + ++   S  TL G+NKGA+M + S + K
Sbjct: 315 RVTNREGSSSMSRKIKEDIRDGISKLTWGKSNGDDKSVSVYTLTGENKGATMGIGSEKDK 374

Query: 494 RESSIHIHRDYKSDPDQSPESESSTDDEGNFNNETPQESRTEEDPPLELYININVQGINN 553
           ++  +HI R Y+S+PD+S  + ++         E P++   EE+     YIN N QGINN
Sbjct: 375 KDGEVHIRRGYRSNPDESSNTTAT-------ETENPKDDEAEEEASFTAYINGNTQGINN 434

Query: 554 SILSNTSFTENNPGIKLKFVREPTRSEDELQSHDAREAK-YTAKPAERLTYEPTVRRRCL 613
           SI+  +S +EN+PG+ + F  E  + E      +  E K  T    ++L  EP VRRRCL
Sbjct: 435 SIVVESSVSENDPGVHMSFKFEILKKEVIYPPENVEEKKPETVTVTKKLKNEPRVRRRCL 494

Query: 614 RGLLMESSDSELDDPEKPRRHGCRYSRGCEGKEVE 635
           RGLL ESS+SE D+P KPRRHGCR++  C+ K++E
Sbjct: 495 RGLLAESSESEPDNPLKPRRHGCRFT--CKDKDIE 511

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
XP_023002262.13.0e-20169.66cell wall protein RBR3-like [Cucurbita maxima][more]
XP_022951875.16.7e-20169.77cell wall protein RBR3-like [Cucurbita moschata][more]
XP_023537866.11.3e-19969.66cell wall protein RBR3-like [Cucurbita pepo subsp. pepo][more]
KAG7020502.14.8e-19969.25hypothetical protein SDJN02_17187, partial [Cucurbita argyrosperma subsp. argyro... [more]
KAG6585587.11.4e-19869.10hypothetical protein SDJN03_18320, partial [Cucurbita argyrosperma subsp. sorori... [more]
Match NameE-valueIdentityDescription
Match NameE-valueIdentityDescription
A0A6J1KJ101.5e-20169.66cell wall protein RBR3-like OS=Cucurbita maxima OX=3661 GN=LOC111496164 PE=4 SV=... [more]
A0A6J1GK503.3e-20169.77cell wall protein RBR3-like OS=Cucurbita moschata OX=3662 GN=LOC111454611 PE=4 S... [more]
A0A5A7VAN02.0e-15853.47Flocculation protein FLO11 OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaff... [more]
A0A6J1CRH02.5e-12958.48cell wall protein RBR3 OS=Momordica charantia OX=3673 GN=LOC111013918 PE=4 SV=1[more]
A0A0A0LLH12.9e-11765.92Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_2G361770 PE=4 SV=1[more]
Match NameE-valueIdentityDescription
AT1G75260.14.3e-2028.16oxidoreductases, acting on NADH or NADPH [more]
InterPro
Analysis Name: InterPro Annotations of Sponge gourd (AG-4) v1
Date Performed: 2022-08-01
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
NoneNo IPR availableCOILSCoilCoilcoord: 385..405
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 1..526
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 423..441
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 473..499
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 35..52
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 385..399
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 215..291
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 78..93
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 346..384
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 94..205
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 400..422
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 604..624
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 299..315
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 316..345
NoneNo IPR availablePANTHERPTHR33472:SF15MUCIN-2-LIKEcoord: 450..629
NoneNo IPR availablePANTHERPTHR33472OS01G0106600 PROTEINcoord: 450..629

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Lag0004395.1Lag0004395.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0016310 phosphorylation
molecular_function GO:0016301 kinase activity