Lag0004394 (gene) Sponge gourd (AG‐4) v1

Overview
NameLag0004394
Typegene
OrganismLuffa acutangula (Sponge gourd (AG‐4) v1)
DescriptionPectinesterase
Locationchr6: 3474344 .. 3478048 (-)
RNA-Seq ExpressionLag0004394
SyntenyLag0004394
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: polypeptideexonCDS
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGTCTCGTCGGAACGGTTTCTCGCTGGTTTTTTATACAGCGATCGCGATTTGTGTTCTCGTGGCGGAGGTTGCAGCGGCGACGACGGCGATTCTAATCACCGTCGATCAATCCGGTAAAGGAAATTTTACGAAGATTCAAGACGCGATCGACGCGGTGCCGGAAAATAATTCGGAGGAGGTGTTTATCGCGGTGAAGCCGGGGATTTACAGAGAGAAGGTGATCGTTCCGGCGAATAAACCGTACATCACGATCAGTGGACGAAAGGCGGTTGATACGATCATCACTTGGAATGATAGCAAAAATACTTATAATTCTGCAACTCTTGCGGTTTTGGCCTCTGATTTCGTTGGAAGATACCTCACAATTCAGGTTTTAATTTCTACGGATGAGAATTCTTTGGTTTTATTTGGCTTCTATGTGATTTTTCGTTGCTCAAAATTTTCTCCGTTTCTCTGTTTTGATTCTTCAACTTCTAACATTTTGAGTATTTTTCTTGTTCATCAACTCTTTCTCTCTCGTTTTGCAAAAAAGAATCAGAAAGAATCTCTCTCTATCTATAGCTTTTGTAAAGTTTTTTTTGTTGGTTTTCATAATTTAGCTAATAATTTTGAAAAGTTTCGAAAAGTAATTTACTATACAAAATAATAGGATATTGTATGAATAATATTTTTTTGTCAAAGCATTTGTTCAACTAATTAGACCACAAAAGATCGAGTCCTCATGAACTAAAAGAAAGTGTAAGATTTTTCAAAATAGAAATCAAGAATGAAAAAATTCTTTATGAATTTTATTTTGATTTTTTCGGATCTTATTTAATAACCATTTTTACTTTACTTTCATTCATCTTTTAAATGGAAGTTAATTTCTAATTTTTTTTTTTTTTTGTCCTTATTGGTTTACTTTATTAAAATCACACTTTTGAACCTTCAACAAAATTTAAAAGAAAAAAACGTTATTGAAAAATATGAGTTTTGTAGTTTTTCAAAATACGTTACAAAAGAATATTCTTATTTCTAGGTAAACTCTAAATTTCTATTCACTCAAATGGATCCTATTGAGATTTGACTTTAGAACGATAGTCTAAATGTAATATGAATAATAAATAATGGGAATGTTATATCAAGCTTTCACCAAACATTTTTACTAAAAATCAAAATGAGGAGAGTGAAACGTTTGTTAAGAAAACCAAATTCATTCTTCAAAAAAAAAAAAAAAAAAAAAAAAAAACCAAATTCATTTGATAATATTGGTTAAATTATTAAAAATACCCATAAAATATGAGGTTGGTTTCAATTATATTTTGAATATTCAAAAGTTTCATTTTAATTTTAAAATTTGAAGTTTGTTTCAAAATAACTTTCGAAGACATTTTCCATTAGATTTATGGACGAAAAATTATATGTTTGCATCTGTTTTTATTGTTTTATCCACGTATGTGTTCAAATCTATTCATATAAGCAATAATACATATGTAAACATGTCATTTTTGTCCATGAATTTGACGTAAAAAAGTCTTCAAATATTGTTTTGAAACAAATTCAAACTTCAAAAGTTAAAGACCCTGTTTGATAACCATTTAGTTTTTGGTTTTTTGCTTTTGAAAATTAAGTTTTACATACATACTTCCACCGATAAGTTTCTTGGTTTTAGTATCAACCTTGTTTTCAAACACCAAATCCAATTTTGGAAACAAAAAAAAAAAAAATAGTTTTCAAAAACTTGTTTTTGTTTTTAAATTTTGACTAGAAATTTAAATGCAACATTAACTAGAATGAAAGTCATGATAGGAAATTTAGAAGAAAACAAGCATAATTTTAAAAAACCAAATAATTATCAAACAAGGCCTAAAATGAAACTTTTAAAAGTTAAGAAGTAAATAATTGAAACCAAACCCACAGTTAAAGGAGTATTTTTGATAATTTAACCATAATTAAAAATTACACATAGTTCTAACTATTAAGCTATGATGGAGGGAAAAAATAGGGAATATAAAAAACATATTTAAATTTAAGTTGTGTACTTTTGTGTTATTCATTAAAAGAGTAATTTGAATCTGACTTGTTTATTTCAATTATGGGGTTGAAGAACGGGTTCGGGGCGGGTGCACAAGCGGTAGCATTACGGGTATCCGGAGACCGAGTGTCATTCGTGGCCTGCAGATTTGTTGGGCATCAAGACACTCTTCTAGACGACATCGGCCGCCATTACTACAAAAGCTGTTACATCGAAGGAGCCACCGATTTCATCTGCGGAAACGGGGCCTCACTTTTCGAGGTTCCAATCCAAAAGCCAACCTTCTCTCTCTTTTCTTTTATTGTTTTTCAATTGGGCTGCCTATTGGGCCACGATAGGTTAATGGGCCTGTTGGGTTTGGGCTTTTGCAGAATTGCCACCTGCGATCGGTGTCGGATGCGGTGGGGACCATCACGGCCCAACGGAGAGAGTCGCCGTCGGAGAATACGGGGTTCGTGTTCATGGGGTGCAAGATAAGTGGGATTAACTCCGCCGTTTTGGGAAGGCCGTGGGGGGCCTTCTCAAGGGTCGTGTTTGGATACACTTATATGTCGGGTGTTATTCTGCCCGAGGGTTGGGATGACTGGCAAGACCCATCCAAACACAGGTTTCTACTCTTCTCTTCTCCTTTTTTATTTCTTTAAATAAAAATAAAACCAAATTTAATATTAAAAATTTAGTCCTATTTGATAAAATCTCGTTTGATAATAATTTGGTTTTTGAAATTATACTTGTTTTTTCTTGATTTCTTTACCATAGTTTTTATTATTGTCAATAATACTTTTGAATTCCTAACCAAATTAAAAAAATATATTTAAAACTATTTTTTTTTTTCAAAATTTGACTTGGTTCCAAAAAATAAAAAGGATATGGATAATAAAACAAAGAAACTTGTGGGTGAAATTAATATTTATAAGCTTAATTAAAAAAAAAAACAAAAAACAACAAACGATATGAATAACAAAACAAAGAAACTTATGTGTGAAAGTAGTATTTATAAGCTTAATTTTAAAAAAATAAATAACAAAAAACGACTACACTCTTGAAAATAAAACAAAGATACTTGTTTTTTCTTAATTTCCTTACCATAGTTTTCATCGTTTTTTGTTTTTAGTTTTTGAAATTAAGCTTGTTTTCTTCCAATCTTTTTCCAATATCTCTTTGGGAGAGTTAATATTGATTATTAAATAACACTTCACTTTTGAAAATGAACGTGCATGTTTCATAGTGATTTTAAAATGGCTAAAATCACTTTTTTTTTTTTTTCTTCTTCTTCATCAGAATCACCTCAAAACATATTTTTAATTATTCAAAGTCAATTCCATGTTTGATTTTACGCTTTATTTTAAACGAGATTTTCATATCATCAAAATTGATTTTGAATTATTAAAGACGTATTTCAAAGTAATTTTGAATATGATAAAAGTGATTTTAACCATTTCAAAATCACTCCCAAACGTACACGAACCAATTAACTATTTGAAAATGTATTTGAGCAGCACTGTTTACTATGGGCAATATAGATGCTATGGAAAAGGAGCCAATCCATCAAGAAGGGTACCATGGTCATTTACAAACATGACCAGCCAAGAAGCTGCACCATTCTTCACCAAAACCTTCATTGGCGCTGCAGATTGGCTCAGGCCTTTGCCAACCACTTTCAAGAGAACCTTCTCTTCCAAACCCTAA

mRNA sequence

ATGTCTCGTCGGAACGGTTTCTCGCTGGTTTTTTATACAGCGATCGCGATTTGTGTTCTCGTGGCGGAGGTTGCAGCGGCGACGACGGCGATTCTAATCACCGTCGATCAATCCGGTAAAGGAAATTTTACGAAGATTCAAGACGCGATCGACGCGGTGCCGGAAAATAATTCGGAGGAGGTGTTTATCGCGGTGAAGCCGGGGATTTACAGAGAGAAGGTGATCGTTCCGGCGAATAAACCGTACATCACGATCAGTGGACGAAAGGCGGTTGATACGATCATCACTTGGAATGATAGCAAAAATACTTATAATTCTGCAACTCTTGCGGTTTTGGCCTCTGATTTCGTTGGAAGATACCTCACAATTCAGAACGGGTTCGGGGCGGGTGCACAAGCGGTAGCATTACGGGTATCCGGAGACCGAGTGTCATTCGTGGCCTGCAGATTTGTTGGGCATCAAGACACTCTTCTAGACGACATCGGCCGCCATTACTACAAAAGCTGTTACATCGAAGGAGCCACCGATTTCATCTGCGGAAACGGGGCCTCACTTTTCGAGAATTGCCACCTGCGATCGGTGTCGGATGCGGTGGGGACCATCACGGCCCAACGGAGAGAGTCGCCGTCGGAGAATACGGGGTTCGTGTTCATGGGGTGCAAGATAAGTGGGATTAACTCCGCCGTTTTGGGAAGGCCGTGGGGGGCCTTCTCAAGGGTCGTGTTTGGATACACTTATATGTCGGGTGTTATTCTGCCCGAGGGTTGGGATGACTGGCAAGACCCATCCAAACACAGCACTGTTTACTATGGGCAATATAGATGCTATGGAAAAGGAGCCAATCCATCAAGAAGGGTACCATGGTCATTTACAAACATGACCAGCCAAGAAGCTGCACCATTCTTCACCAAAACCTTCATTGGCGCTGCAGATTGGCTCAGGCCTTTGCCAACCACTTTCAAGAGAACCTTCTCTTCCAAACCCTAA

Coding sequence (CDS)

ATGTCTCGTCGGAACGGTTTCTCGCTGGTTTTTTATACAGCGATCGCGATTTGTGTTCTCGTGGCGGAGGTTGCAGCGGCGACGACGGCGATTCTAATCACCGTCGATCAATCCGGTAAAGGAAATTTTACGAAGATTCAAGACGCGATCGACGCGGTGCCGGAAAATAATTCGGAGGAGGTGTTTATCGCGGTGAAGCCGGGGATTTACAGAGAGAAGGTGATCGTTCCGGCGAATAAACCGTACATCACGATCAGTGGACGAAAGGCGGTTGATACGATCATCACTTGGAATGATAGCAAAAATACTTATAATTCTGCAACTCTTGCGGTTTTGGCCTCTGATTTCGTTGGAAGATACCTCACAATTCAGAACGGGTTCGGGGCGGGTGCACAAGCGGTAGCATTACGGGTATCCGGAGACCGAGTGTCATTCGTGGCCTGCAGATTTGTTGGGCATCAAGACACTCTTCTAGACGACATCGGCCGCCATTACTACAAAAGCTGTTACATCGAAGGAGCCACCGATTTCATCTGCGGAAACGGGGCCTCACTTTTCGAGAATTGCCACCTGCGATCGGTGTCGGATGCGGTGGGGACCATCACGGCCCAACGGAGAGAGTCGCCGTCGGAGAATACGGGGTTCGTGTTCATGGGGTGCAAGATAAGTGGGATTAACTCCGCCGTTTTGGGAAGGCCGTGGGGGGCCTTCTCAAGGGTCGTGTTTGGATACACTTATATGTCGGGTGTTATTCTGCCCGAGGGTTGGGATGACTGGCAAGACCCATCCAAACACAGCACTGTTTACTATGGGCAATATAGATGCTATGGAAAAGGAGCCAATCCATCAAGAAGGGTACCATGGTCATTTACAAACATGACCAGCCAAGAAGCTGCACCATTCTTCACCAAAACCTTCATTGGCGCTGCAGATTGGCTCAGGCCTTTGCCAACCACTTTCAAGAGAACCTTCTCTTCCAAACCCTAA

Protein sequence

MSRRNGFSLVFYTAIAICVLVAEVAAATTAILITVDQSGKGNFTKIQDAIDAVPENNSEEVFIAVKPGIYREKVIVPANKPYITISGRKAVDTIITWNDSKNTYNSATLAVLASDFVGRYLTIQNGFGAGAQAVALRVSGDRVSFVACRFVGHQDTLLDDIGRHYYKSCYIEGATDFICGNGASLFENCHLRSVSDAVGTITAQRRESPSENTGFVFMGCKISGINSAVLGRPWGAFSRVVFGYTYMSGVILPEGWDDWQDPSKHSTVYYGQYRCYGKGANPSRRVPWSFTNMTSQEAAPFFTKTFIGAADWLRPLPTTFKRTFSSKP
Homology
BLAST of Lag0004394 vs. NCBI nr
Match: XP_023536774.1 (putative pectinesterase 11 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 594.7 bits (1532), Expect = 4.7e-166
Identity = 294/330 (89.09%), Postives = 307/330 (93.03%), Query Frame = 0

Query: 1   MSRRNGFSLVFYTAIAICVLVAE--VAAATTAILITVDQSGKGNFTKIQDAIDAVPENNS 60
           MS   GF L   TAI ICVLVAE   A A TAILITVDQSG+GNFTKIQ+AIDAVPENN 
Sbjct: 1   MSSSRGF-LQACTAIVICVLVAEFTAAMAKTAILITVDQSGRGNFTKIQNAIDAVPENNL 60

Query: 61  EEVFIAVKPGIYREKVIVPANKPYITISGRKAVDTIITWNDSKNTYNSATLAVLASDFVG 120
           EEVFIAVKPGIYREKV VPANKPYITISGR++ DTIITWNDS+NTYNSATLAVLASDFVG
Sbjct: 61  EEVFIAVKPGIYREKVTVPANKPYITISGRRSSDTIITWNDSRNTYNSATLAVLASDFVG 120

Query: 121 RYLTIQNGFGAGAQAVALRVSGDRVSFVACRFVGHQDTLLDDIGRHYYKSCYIEGATDFI 180
           RYLTIQNGFGAGAQAVALRVSGDRVSF ACRF+GHQDTLLDDIGRHYYKSCYIEGATDFI
Sbjct: 121 RYLTIQNGFGAGAQAVALRVSGDRVSFTACRFLGHQDTLLDDIGRHYYKSCYIEGATDFI 180

Query: 181 CGNGASLFENCHLRSVSDAVGTITAQRRESPSENTGFVFMGCKISGINSAVLGRPWGAFS 240
           CGNGASLFENCHL SVSD+VGTITAQRRESPSENTGFVFMGCKI+GINSAVLGRPWGAFS
Sbjct: 181 CGNGASLFENCHLHSVSDSVGTITAQRRESPSENTGFVFMGCKITGINSAVLGRPWGAFS 240

Query: 241 RVVFGYTYMSGVILPEGWDDWQDPSKHSTVYYGQYRCYGKGANPSRRVPWSFTNMTSQEA 300
           RVVFG+TYMS VILPEGWD+WQDPSK STVYYGQYRCYGKGANPSRRVPWSFTNMT+QEA
Sbjct: 241 RVVFGFTYMSDVILPEGWDNWQDPSKQSTVYYGQYRCYGKGANPSRRVPWSFTNMTTQEA 300

Query: 301 APFFTKTFIGAADWLRPLPTTFKRTFSSKP 329
           APFF+KTFIGAA WLRPLPT FKR F+SKP
Sbjct: 301 APFFSKTFIGAAHWLRPLPTNFKRAFASKP 329

BLAST of Lag0004394 vs. NCBI nr
Match: XP_022951672.1 (putative pectinesterase 11 [Cucurbita moschata])

HSP 1 Score: 594.3 bits (1531), Expect = 6.2e-166
Identity = 293/330 (88.79%), Postives = 307/330 (93.03%), Query Frame = 0

Query: 1   MSRRNGFSLVFYTAIAICVLVAE--VAAATTAILITVDQSGKGNFTKIQDAIDAVPENNS 60
           MSR  GF     TAI ICVLVAE   A A TAILITVDQSG+GNFTKIQ+AIDAVPENN 
Sbjct: 1   MSRSRGF-FQACTAIVICVLVAEFTAAMAKTAILITVDQSGRGNFTKIQNAIDAVPENNL 60

Query: 61  EEVFIAVKPGIYREKVIVPANKPYITISGRKAVDTIITWNDSKNTYNSATLAVLASDFVG 120
           EEVFIAVKPGIYREKV VPANKPYITISGR++ DTIITWNDS+NTYNSATLAVLASDFVG
Sbjct: 61  EEVFIAVKPGIYREKVTVPANKPYITISGRRSSDTIITWNDSRNTYNSATLAVLASDFVG 120

Query: 121 RYLTIQNGFGAGAQAVALRVSGDRVSFVACRFVGHQDTLLDDIGRHYYKSCYIEGATDFI 180
           RYLTIQNGFGAGAQAVALRVSGDRVSF ACRF+GHQDTLLDDIGRHYY+SCYIEGATDFI
Sbjct: 121 RYLTIQNGFGAGAQAVALRVSGDRVSFTACRFLGHQDTLLDDIGRHYYRSCYIEGATDFI 180

Query: 181 CGNGASLFENCHLRSVSDAVGTITAQRRESPSENTGFVFMGCKISGINSAVLGRPWGAFS 240
           CGNGASLFENCHL SVSD+VGTITAQRRESPSENTGFVFMGCKI+GINSAVLGRPWGAFS
Sbjct: 181 CGNGASLFENCHLHSVSDSVGTITAQRRESPSENTGFVFMGCKITGINSAVLGRPWGAFS 240

Query: 241 RVVFGYTYMSGVILPEGWDDWQDPSKHSTVYYGQYRCYGKGANPSRRVPWSFTNMTSQEA 300
           RVVFG+TYMS VILPEGWD+WQDPSK STVYYGQYRCYGKGANPSRRVPWSFTNMT+QEA
Sbjct: 241 RVVFGFTYMSDVILPEGWDNWQDPSKQSTVYYGQYRCYGKGANPSRRVPWSFTNMTTQEA 300

Query: 301 APFFTKTFIGAADWLRPLPTTFKRTFSSKP 329
           APFF+KTFIGAA WLRPLPT FKR F+SKP
Sbjct: 301 APFFSKTFIGAAHWLRPLPTNFKRAFASKP 329

BLAST of Lag0004394 vs. NCBI nr
Match: KAG6585588.1 (putative pectinesterase 11, partial [Cucurbita argyrosperma subsp. sororia] >KAG7020503.1 putative pectinesterase 11, partial [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 592.8 bits (1527), Expect = 1.8e-165
Identity = 292/330 (88.48%), Postives = 306/330 (92.73%), Query Frame = 0

Query: 1   MSRRNGFSLVFYTAIAICVLVAE--VAAATTAILITVDQSGKGNFTKIQDAIDAVPENNS 60
           MSR  GF     TAI ICVLVAE   A A TAILITVDQSG+GNFTKIQ+AID VPENN 
Sbjct: 1   MSRSRGF-FQACTAIVICVLVAEFTAAMAKTAILITVDQSGRGNFTKIQNAIDVVPENNL 60

Query: 61  EEVFIAVKPGIYREKVIVPANKPYITISGRKAVDTIITWNDSKNTYNSATLAVLASDFVG 120
           EEVFIAVKPGIYREKV VPANKPYITISGR++ DTIITWNDS+NTYNSATLAVLASDFVG
Sbjct: 61  EEVFIAVKPGIYREKVTVPANKPYITISGRRSSDTIITWNDSRNTYNSATLAVLASDFVG 120

Query: 121 RYLTIQNGFGAGAQAVALRVSGDRVSFVACRFVGHQDTLLDDIGRHYYKSCYIEGATDFI 180
           RYLTIQNGFGAGAQAVALRVSGDRVSF ACRF+GHQDTLLDDIGRHYY+SCYIEGATDFI
Sbjct: 121 RYLTIQNGFGAGAQAVALRVSGDRVSFTACRFLGHQDTLLDDIGRHYYRSCYIEGATDFI 180

Query: 181 CGNGASLFENCHLRSVSDAVGTITAQRRESPSENTGFVFMGCKISGINSAVLGRPWGAFS 240
           CGNGASLFENCHL SVSD+VGTITAQRRESPSENTGFVFMGCKI+GINSAVLGRPWGAFS
Sbjct: 181 CGNGASLFENCHLHSVSDSVGTITAQRRESPSENTGFVFMGCKITGINSAVLGRPWGAFS 240

Query: 241 RVVFGYTYMSGVILPEGWDDWQDPSKHSTVYYGQYRCYGKGANPSRRVPWSFTNMTSQEA 300
           RVVFG+TYMS VILPEGWD+WQDPSK STVYYGQYRCYGKGANPSRRVPWSFTNMT+QEA
Sbjct: 241 RVVFGFTYMSDVILPEGWDNWQDPSKQSTVYYGQYRCYGKGANPSRRVPWSFTNMTTQEA 300

Query: 301 APFFTKTFIGAADWLRPLPTTFKRTFSSKP 329
           APFF+KTFIGAA WLRPLPT FKR F+SKP
Sbjct: 301 APFFSKTFIGAAHWLRPLPTNFKRAFASKP 329

BLAST of Lag0004394 vs. NCBI nr
Match: XP_023002424.1 (putative pectinesterase 11 [Cucurbita maxima])

HSP 1 Score: 592.8 bits (1527), Expect = 1.8e-165
Identity = 292/330 (88.48%), Postives = 307/330 (93.03%), Query Frame = 0

Query: 1   MSRRNGFSLVFYTAIAICVLVAE--VAAATTAILITVDQSGKGNFTKIQDAIDAVPENNS 60
           MSR  GF     TAI ICVLVAE   A A TAILITVDQSG+GNFTKIQ+AIDAVP+NN 
Sbjct: 1   MSRSRGF-FQACTAIVICVLVAEFTAAMAKTAILITVDQSGRGNFTKIQNAIDAVPDNNL 60

Query: 61  EEVFIAVKPGIYREKVIVPANKPYITISGRKAVDTIITWNDSKNTYNSATLAVLASDFVG 120
           EEVFIAVKPGIYREKV VPANKPYITISGR++ DTIITWNDS+NTYNSATLAVLASDFVG
Sbjct: 61  EEVFIAVKPGIYREKVTVPANKPYITISGRRSSDTIITWNDSRNTYNSATLAVLASDFVG 120

Query: 121 RYLTIQNGFGAGAQAVALRVSGDRVSFVACRFVGHQDTLLDDIGRHYYKSCYIEGATDFI 180
           RYLTIQNGFGAGAQAVALRVSGDRVSF ACRF+GHQDTLLDDIGRHYYKSCYIEGATDFI
Sbjct: 121 RYLTIQNGFGAGAQAVALRVSGDRVSFTACRFLGHQDTLLDDIGRHYYKSCYIEGATDFI 180

Query: 181 CGNGASLFENCHLRSVSDAVGTITAQRRESPSENTGFVFMGCKISGINSAVLGRPWGAFS 240
           CGNGASLFENCHL SVSD+VGTITAQRRESPSENTGFVFMGCKI+GINSAVLGRPWGAFS
Sbjct: 181 CGNGASLFENCHLHSVSDSVGTITAQRRESPSENTGFVFMGCKITGINSAVLGRPWGAFS 240

Query: 241 RVVFGYTYMSGVILPEGWDDWQDPSKHSTVYYGQYRCYGKGANPSRRVPWSFTNMTSQEA 300
           RVVFG+TYMS VILPEGWD+WQDPSK STVYYGQYRCYGKGANPSRRVPWSF+NMT+QEA
Sbjct: 241 RVVFGFTYMSDVILPEGWDNWQDPSKQSTVYYGQYRCYGKGANPSRRVPWSFSNMTTQEA 300

Query: 301 APFFTKTFIGAADWLRPLPTTFKRTFSSKP 329
           APFF+KTFIGAA WLRPLPT FKR F+SKP
Sbjct: 301 APFFSKTFIGAAHWLRPLPTNFKRAFASKP 329

BLAST of Lag0004394 vs. NCBI nr
Match: XP_022144190.1 (putative pectinesterase 11 [Momordica charantia])

HSP 1 Score: 585.5 bits (1508), Expect = 2.9e-163
Identity = 287/333 (86.19%), Postives = 306/333 (91.89%), Query Frame = 0

Query: 1   MSRRNGF---SLVFYTAIAICVLVAEV--AAATTAILITVDQSGKGNFTKIQDAIDAVPE 60
           MSRR+ F   + +   ++ + V VAE+  AAAT AILITVDQSG+GNFTKIQDAIDAVP 
Sbjct: 1   MSRRSDFPRITTIALISVFVAVFVAEITKAAATAAILITVDQSGRGNFTKIQDAIDAVPV 60

Query: 61  NNSEEVFIAVKPGIYREKVIVPANKPYITISGRKAVDTIITWNDSKNTYNSATLAVLASD 120
           NN E+VFIAVKPGIYREKVIVPANKP+ITISGR+AVDTI+TWNDS+ TYNSATLAVLASD
Sbjct: 61  NNVEQVFIAVKPGIYREKVIVPANKPFITISGRRAVDTIVTWNDSRITYNSATLAVLASD 120

Query: 121 FVGRYLTIQNGFGAGAQAVALRVSGDRVSFVACRFVGHQDTLLDDIGRHYYKSCYIEGAT 180
           FVGRYLTIQNGFG GAQAVALRVSGDRVSF ACRF+GHQDTLLDDIGRHYYKSCYIEGAT
Sbjct: 121 FVGRYLTIQNGFGPGAQAVALRVSGDRVSFNACRFLGHQDTLLDDIGRHYYKSCYIEGAT 180

Query: 181 DFICGNGASLFENCHLRSVSDAVGTITAQRRESPSENTGFVFMGCKISGINSAVLGRPWG 240
           DFICGN ASLFENCHL SVSDAVGTITAQRRESPSE TGF FMGCKI+GINSAVLGRPWG
Sbjct: 181 DFICGNAASLFENCHLHSVSDAVGTITAQRRESPSETTGFSFMGCKITGINSAVLGRPWG 240

Query: 241 AFSRVVFGYTYMSGVILPEGWDDWQDPSKHSTVYYGQYRCYGKGANPSRRVPWSFTNMTS 300
           A+SRVVFGYTYMS +ILP+GWDDWQDPSKHSTVYYGQYRCYGKGANPSRRVPWS TNMTS
Sbjct: 241 AYSRVVFGYTYMSDLILPQGWDDWQDPSKHSTVYYGQYRCYGKGANPSRRVPWSLTNMTS 300

Query: 301 QEAAPFFTKTFIGAADWLRPLPTTFKRTFSSKP 329
           QEAAPFF K+FIGAADWLRPLPTTFKR FSSKP
Sbjct: 301 QEAAPFFNKSFIGAADWLRPLPTTFKRAFSSKP 333

BLAST of Lag0004394 vs. ExPASy Swiss-Prot
Match: Q9SIJ9 (Putative pectinesterase 11 OS=Arabidopsis thaliana OX=3702 GN=PME11 PE=3 SV=1)

HSP 1 Score: 368.2 bits (944), Expect = 9.4e-101
Identity = 170/296 (57.43%), Postives = 223/296 (75.34%), Query Frame = 0

Query: 28  TTAILITVDQSGKGNFTKIQDAIDAVPE--NNSEEVFIAVKPGIYREKVIVPANKPYITI 87
           +TAILI VDQSGKG+F+KIQ+AI+++P   NNS+  FI VKPGIYREKV++PA KPYIT+
Sbjct: 46  STAILIRVDQSGKGDFSKIQEAIESIPPNLNNSQLYFIWVKPGIYREKVVIPAEKPYITL 105

Query: 88  SGRKAVDTIITWNDSKNTYNSATLAVLASDFVGRYLTIQNGFGAGAQAVALRVSGDRVSF 147
           SG +A +T + W+D ++   S TL + ASDFV R+LTIQN FG   +AVALRV+ D+ +F
Sbjct: 106 SGTQASNTFLIWSDGEDILESPTLTIFASDFVCRFLTIQNKFGTAGRAVALRVAADKAAF 165

Query: 148 VACRFVGHQDTLLDDIGRHYYKSCYIEGATDFICGNGASLFENCHLRSVSDAVGTITAQR 207
             C    +QDTLLDD G HY+K+CYIEGATDFICG+ +SL+E CHL S+S   G+ITAQ 
Sbjct: 166 YGCVITSYQDTLLDDNGNHYFKNCYIEGATDFICGSASSLYERCHLHSLSPNNGSITAQM 225

Query: 208 RESPSENTGFVFMGCKISGINSAVLGRPWGAFSRVVFGYTYMSGVILPEGWDDWQDPSKH 267
           R S +E +GF F+GCK++G  S  LGRPWGA+SRVVF Y++ S V+ P+GW+ W D +K 
Sbjct: 226 RTSATEKSGFTFLGCKLTGSGSTFLGRPWGAYSRVVFAYSFFSNVVAPQGWNQWGDSTKE 285

Query: 268 STVYYGQYRCYGKGANPSRRVPWSFTNMTSQEAAPFFTKTFIGAADWLRPLPTTFK 322
           +TVYYG+Y+CYG GA+  +RV WS   ++ +EA  F +K FIG  DWLRP P+ FK
Sbjct: 286 NTVYYGEYKCYGPGADREQRVEWS-KQLSDEEATVFLSKDFIGGKDWLRPAPSHFK 340

BLAST of Lag0004394 vs. ExPASy Swiss-Prot
Match: Q8VYZ3 (Probable pectinesterase 53 OS=Arabidopsis thaliana OX=3702 GN=PME53 PE=2 SV=1)

HSP 1 Score: 296.2 bits (757), Expect = 4.6e-79
Identity = 145/294 (49.32%), Postives = 188/294 (63.95%), Query Frame = 0

Query: 37  QSGKGNFTKIQDAIDAVPENNSEEVFIAVKPGIYREKVIVPANKPYITISGRKAVDTIIT 96
           +S KG+FTKIQDAID++P  N   V I V  G+Y+EKV +P  K +ITI G  A  T + 
Sbjct: 90  KSNKGDFTKIQDAIDSLPLINFVRVVIKVHAGVYKEKVSIPPLKAFITIEGEGAEKTTVE 149

Query: 97  WNDS----------KNTYNSATLAVLASDFVGRYLTIQNGF------GAGAQAVALRVSG 156
           W D+            TYNSA+ AV +  FV + +T +N          G QAVALRVS 
Sbjct: 150 WGDTAQTPDSKGNPMGTYNSASFAVNSPFFVAKNITFRNTTPVPLPGAVGKQAVALRVSA 209

Query: 157 DRVSFVACRFVGHQDTLLDDIGRHYYKSCYIEGATDFICGNGASLFENCHLRSVSDAVGT 216
           D  +F  CR +G QDTL D +GRHYYK CYIEG+ DFI GN  SL+E CH+ +++D +G 
Sbjct: 210 DNAAFFGCRMLGAQDTLYDHLGRHYYKDCYIEGSVDFIFGNALSLYEGCHVHAIADKLGA 269

Query: 217 ITAQRRESPSENTGFVFMGCKISGINSAVLGRPWGAFSRVVFGYTYMSGVILPEGWDDWQ 276
           +TAQ R S  E+TGF F+ CK++G     LGR WG FSRVVF YTYM  +ILP GW +W 
Sbjct: 270 VTAQGRSSVLEDTGFSFVKCKVTGTGVLYLGRAWGPFSRVVFAYTYMDNIILPRGWYNWG 329

Query: 277 DPSKHSTVYYGQYRCYGKGANPSRRVPWSFTNMTSQEAAPFFTKTFIGAADWLR 315
           DPS+  TV+YGQY+C G GAN   RV W+   +T +EA PF + TFI  ++W++
Sbjct: 330 DPSREMTVFYGQYKCTGAGANYGGRVAWA-RELTDEEAKPFLSLTFIDGSEWIK 382

BLAST of Lag0004394 vs. ExPASy Swiss-Prot
Match: Q9ZQA3 (Probable pectinesterase 15 OS=Arabidopsis thaliana OX=3702 GN=PME15 PE=2 SV=1)

HSP 1 Score: 267.7 bits (683), Expect = 1.7e-70
Identity = 136/301 (45.18%), Postives = 189/301 (62.79%), Query Frame = 0

Query: 29  TAILITVDQSGKGNFTKIQDAIDAVPENNSEEVFIAVKPGIYREKVIVPANKPYITISGR 88
           T++++TVD  G GNF+ +Q AID VP+ +S +  I V  G YREKV V  NK  + I GR
Sbjct: 87  TSLVLTVDLHGCGNFSNVQSAIDVVPDLSSSKTLIIVNSGSYREKVTVNENKTNLVIQGR 146

Query: 89  KAVDTIITWNDSK----NTYNSATLAVLASDFVGRYLTIQNGF------GAGAQAVALRV 148
              +T I WND+     NT +S +  V A++F    ++ +N         A AQAVALR+
Sbjct: 147 GYQNTSIEWNDTAKSAGNTADSFSFVVFAANFTAYNISFKNNAPEPDPGEADAQAVALRI 206

Query: 149 SGDRVSFVACRFVGHQDTLLDDIGRHYYKSCYIEGATDFICGNGASLFENCHLRSV---- 208
            GD+ +F  C F G QDTLLDD GRH++K C+I+G+ DFI GNG SL+++C + S+    
Sbjct: 207 EGDQAAFYGCGFYGAQDTLLDDKGRHFFKECFIQGSIDFIFGNGRSLYQDCTINSIAKGN 266

Query: 209 -SDAVGTITAQRRESPSENTGFVFMGCKISGINSAVLGRPWGAFSRVVFGYTYMSGVILP 268
            S   G+ITAQ R+S  E +GF F+ CKI G    +LGR WGA++ VVF  TYMSG+I P
Sbjct: 267 TSGVTGSITAQGRQSEDEQSGFSFVNCKIDGSGEILLGRAWGAYATVVFSNTYMSGIITP 326

Query: 269 EGWDDWQDPSKHSTVYYGQYRCYGKGANPSRRVPWSFTNMTSQEAAPFFTKTFIGAADWL 315
           EGW++W D +K  TV +G+++CYG GA+   RV +    +T  EA+ F   +FI   +WL
Sbjct: 327 EGWNNWGDSTKEKTVTFGEHKCYGPGADYKERVLFG-KQLTDSEASSFIDVSFIDGDEWL 386

BLAST of Lag0004394 vs. ExPASy Swiss-Prot
Match: Q8LPF3 (Probable pectinesterase 68 OS=Arabidopsis thaliana OX=3702 GN=PME68 PE=2 SV=1)

HSP 1 Score: 266.2 bits (679), Expect = 5.1e-70
Identity = 128/301 (42.52%), Postives = 178/301 (59.14%), Query Frame = 0

Query: 32  LITVDQSGKGNFTKIQDAIDAVPENNSEEVFIAVKPGIYREKVIVPANKPYITISGRKAV 91
           +ITV  +G   F  +QDA+D++P+NN++ + I + PG YREKV+VPA KPYIT  G    
Sbjct: 60  VITVSLNGHAQFRSVQDAVDSIPKNNNKSITIKIAPGFYREKVVVPATKPYITFKGAGRD 119

Query: 92  DTIITWNDSKN----------TYNSATLAVLASDFVGRYLTIQNGFGA------GAQAVA 151
            T I W+D  +          TY +A++ V A+ F  R ++  N   A      G QAVA
Sbjct: 120 VTAIEWHDRASDLGANGQQLRTYQTASVTVYANYFTARNISFTNTAPAPLPGMQGWQAVA 179

Query: 152 LRVSGDRVSFVACRFVGHQDTLLDDIGRHYYKSCYIEGATDFICGNGASLFENCHLRSVS 211
            R+SGD+  F  C F G QDTL DD GRHY+K CYIEG+ DFI GNG S++++C L S++
Sbjct: 180 FRISGDKAFFSGCGFYGAQDTLCDDAGRHYFKECYIEGSIDFIFGNGRSMYKDCELHSIA 239

Query: 212 DAVGTITAQRRESPSENTGFVFMGCKISGINSAVLGRPWGAFSRVVFGYTYMSGVILPEG 271
              G+I A  R  P E TGF F+GC+++G     +GR  G +SR+V+ YTY   ++   G
Sbjct: 240 SRFGSIAAHGRTCPEEKTGFAFVGCRVTGTGPLYVGRAMGQYSRIVYAYTYFDALVAHGG 299

Query: 272 WDDWQDPS-KHSTVYYGQYRCYGKGANPSRRVPWSFTNMTSQEAAPFFTKTFIGAADWLR 316
           WDDW   S K  T ++G Y CYG GA  +R V W+   +  + A PF  K+F+    W+ 
Sbjct: 300 WDDWDHKSNKSKTAFFGVYNCYGPGAAATRGVSWA-RALDYESAHPFIAKSFVNGRHWIA 359

BLAST of Lag0004394 vs. ExPASy Swiss-Prot
Match: Q9FM79 (Pectinesterase QRT1 OS=Arabidopsis thaliana OX=3702 GN=QRT1 PE=2 SV=1)

HSP 1 Score: 255.8 bits (652), Expect = 6.8e-67
Identity = 130/310 (41.94%), Postives = 182/310 (58.71%), Query Frame = 0

Query: 22  AEVAAATTAILITVDQSGKGNFTKIQDAIDAVPENNSEEVFIAVKPGIYREKVIVPANKP 81
           A   A     +I VD++G G+   +Q A+D VP++NS+ V I + PGIYREKVIVP +KP
Sbjct: 71  ANANATNVRRVIVVDKNGGGDSVTVQGAVDMVPDSNSQRVKIFILPGIYREKVIVPKSKP 130

Query: 82  YITISGRK--AVDTIITWNDSKN----------TYNSATLAVLASDFVGRYLTIQNGFGA 141
           YI+  G +  A DT+I+W+D  +          TY +A++++ +  F    +T +N   A
Sbjct: 131 YISFIGNESYAGDTVISWSDKASDLGCDGKELGTYRTASVSIESDFFCATAITFENTVVA 190

Query: 142 -----GAQAVALRVSGDRVSFVACRFVGHQDTLLDDIGRHYYKSCYIEGATDFICGNGAS 201
                G QAVALR+ GD+  F   R +G QDTL DD G HY+  CYI+G  DFI GN  S
Sbjct: 191 EAGEQGRQAVALRIIGDKAVFYRVRVLGSQDTLFDDNGSHYFYQCYIQGNVDFIFGNAKS 250

Query: 202 LFENCHLRSVSDAVGTITAQRRESPSENTGFVFMGCKISGINSAVLGRPWGAFSRVVFGY 261
           L+++C + S +   G I A  R+S +E+TGF F+ C ISG     LGR WG +SR V+  
Sbjct: 251 LYQDCDIHSTAKRYGAIAAHHRDSETEDTGFSFVNCDISGTGQIYLGRAWGNYSRTVYSN 310

Query: 262 TYMSGVILPEGWDDWQDPSKHSTVYYGQYRCYGKGANPSRRVPWSFTNMTSQEAAPFFTK 315
            +++ +I P GW DW+ P +   V +G+Y C G+GA    RVPWS T +T  E  PF  +
Sbjct: 311 CFIADIITPVGWSDWKHPERQRKVMFGEYNCRGRGAERGGRVPWSKT-LTRDEVKPFLGR 370

BLAST of Lag0004394 vs. ExPASy TrEMBL
Match: A0A6J1GIB5 (Pectinesterase OS=Cucurbita moschata OX=3662 GN=LOC111454415 PE=4 SV=1)

HSP 1 Score: 594.3 bits (1531), Expect = 3.0e-166
Identity = 293/330 (88.79%), Postives = 307/330 (93.03%), Query Frame = 0

Query: 1   MSRRNGFSLVFYTAIAICVLVAE--VAAATTAILITVDQSGKGNFTKIQDAIDAVPENNS 60
           MSR  GF     TAI ICVLVAE   A A TAILITVDQSG+GNFTKIQ+AIDAVPENN 
Sbjct: 1   MSRSRGF-FQACTAIVICVLVAEFTAAMAKTAILITVDQSGRGNFTKIQNAIDAVPENNL 60

Query: 61  EEVFIAVKPGIYREKVIVPANKPYITISGRKAVDTIITWNDSKNTYNSATLAVLASDFVG 120
           EEVFIAVKPGIYREKV VPANKPYITISGR++ DTIITWNDS+NTYNSATLAVLASDFVG
Sbjct: 61  EEVFIAVKPGIYREKVTVPANKPYITISGRRSSDTIITWNDSRNTYNSATLAVLASDFVG 120

Query: 121 RYLTIQNGFGAGAQAVALRVSGDRVSFVACRFVGHQDTLLDDIGRHYYKSCYIEGATDFI 180
           RYLTIQNGFGAGAQAVALRVSGDRVSF ACRF+GHQDTLLDDIGRHYY+SCYIEGATDFI
Sbjct: 121 RYLTIQNGFGAGAQAVALRVSGDRVSFTACRFLGHQDTLLDDIGRHYYRSCYIEGATDFI 180

Query: 181 CGNGASLFENCHLRSVSDAVGTITAQRRESPSENTGFVFMGCKISGINSAVLGRPWGAFS 240
           CGNGASLFENCHL SVSD+VGTITAQRRESPSENTGFVFMGCKI+GINSAVLGRPWGAFS
Sbjct: 181 CGNGASLFENCHLHSVSDSVGTITAQRRESPSENTGFVFMGCKITGINSAVLGRPWGAFS 240

Query: 241 RVVFGYTYMSGVILPEGWDDWQDPSKHSTVYYGQYRCYGKGANPSRRVPWSFTNMTSQEA 300
           RVVFG+TYMS VILPEGWD+WQDPSK STVYYGQYRCYGKGANPSRRVPWSFTNMT+QEA
Sbjct: 241 RVVFGFTYMSDVILPEGWDNWQDPSKQSTVYYGQYRCYGKGANPSRRVPWSFTNMTTQEA 300

Query: 301 APFFTKTFIGAADWLRPLPTTFKRTFSSKP 329
           APFF+KTFIGAA WLRPLPT FKR F+SKP
Sbjct: 301 APFFSKTFIGAAHWLRPLPTNFKRAFASKP 329

BLAST of Lag0004394 vs. ExPASy TrEMBL
Match: A0A6J1KQF0 (Pectinesterase OS=Cucurbita maxima OX=3661 GN=LOC111496269 PE=4 SV=1)

HSP 1 Score: 592.8 bits (1527), Expect = 8.7e-166
Identity = 292/330 (88.48%), Postives = 307/330 (93.03%), Query Frame = 0

Query: 1   MSRRNGFSLVFYTAIAICVLVAE--VAAATTAILITVDQSGKGNFTKIQDAIDAVPENNS 60
           MSR  GF     TAI ICVLVAE   A A TAILITVDQSG+GNFTKIQ+AIDAVP+NN 
Sbjct: 1   MSRSRGF-FQACTAIVICVLVAEFTAAMAKTAILITVDQSGRGNFTKIQNAIDAVPDNNL 60

Query: 61  EEVFIAVKPGIYREKVIVPANKPYITISGRKAVDTIITWNDSKNTYNSATLAVLASDFVG 120
           EEVFIAVKPGIYREKV VPANKPYITISGR++ DTIITWNDS+NTYNSATLAVLASDFVG
Sbjct: 61  EEVFIAVKPGIYREKVTVPANKPYITISGRRSSDTIITWNDSRNTYNSATLAVLASDFVG 120

Query: 121 RYLTIQNGFGAGAQAVALRVSGDRVSFVACRFVGHQDTLLDDIGRHYYKSCYIEGATDFI 180
           RYLTIQNGFGAGAQAVALRVSGDRVSF ACRF+GHQDTLLDDIGRHYYKSCYIEGATDFI
Sbjct: 121 RYLTIQNGFGAGAQAVALRVSGDRVSFTACRFLGHQDTLLDDIGRHYYKSCYIEGATDFI 180

Query: 181 CGNGASLFENCHLRSVSDAVGTITAQRRESPSENTGFVFMGCKISGINSAVLGRPWGAFS 240
           CGNGASLFENCHL SVSD+VGTITAQRRESPSENTGFVFMGCKI+GINSAVLGRPWGAFS
Sbjct: 181 CGNGASLFENCHLHSVSDSVGTITAQRRESPSENTGFVFMGCKITGINSAVLGRPWGAFS 240

Query: 241 RVVFGYTYMSGVILPEGWDDWQDPSKHSTVYYGQYRCYGKGANPSRRVPWSFTNMTSQEA 300
           RVVFG+TYMS VILPEGWD+WQDPSK STVYYGQYRCYGKGANPSRRVPWSF+NMT+QEA
Sbjct: 241 RVVFGFTYMSDVILPEGWDNWQDPSKQSTVYYGQYRCYGKGANPSRRVPWSFSNMTTQEA 300

Query: 301 APFFTKTFIGAADWLRPLPTTFKRTFSSKP 329
           APFF+KTFIGAA WLRPLPT FKR F+SKP
Sbjct: 301 APFFSKTFIGAAHWLRPLPTNFKRAFASKP 329

BLAST of Lag0004394 vs. ExPASy TrEMBL
Match: A0A6J1CQY5 (Pectinesterase OS=Momordica charantia OX=3673 GN=LOC111013943 PE=4 SV=1)

HSP 1 Score: 585.5 bits (1508), Expect = 1.4e-163
Identity = 287/333 (86.19%), Postives = 306/333 (91.89%), Query Frame = 0

Query: 1   MSRRNGF---SLVFYTAIAICVLVAEV--AAATTAILITVDQSGKGNFTKIQDAIDAVPE 60
           MSRR+ F   + +   ++ + V VAE+  AAAT AILITVDQSG+GNFTKIQDAIDAVP 
Sbjct: 1   MSRRSDFPRITTIALISVFVAVFVAEITKAAATAAILITVDQSGRGNFTKIQDAIDAVPV 60

Query: 61  NNSEEVFIAVKPGIYREKVIVPANKPYITISGRKAVDTIITWNDSKNTYNSATLAVLASD 120
           NN E+VFIAVKPGIYREKVIVPANKP+ITISGR+AVDTI+TWNDS+ TYNSATLAVLASD
Sbjct: 61  NNVEQVFIAVKPGIYREKVIVPANKPFITISGRRAVDTIVTWNDSRITYNSATLAVLASD 120

Query: 121 FVGRYLTIQNGFGAGAQAVALRVSGDRVSFVACRFVGHQDTLLDDIGRHYYKSCYIEGAT 180
           FVGRYLTIQNGFG GAQAVALRVSGDRVSF ACRF+GHQDTLLDDIGRHYYKSCYIEGAT
Sbjct: 121 FVGRYLTIQNGFGPGAQAVALRVSGDRVSFNACRFLGHQDTLLDDIGRHYYKSCYIEGAT 180

Query: 181 DFICGNGASLFENCHLRSVSDAVGTITAQRRESPSENTGFVFMGCKISGINSAVLGRPWG 240
           DFICGN ASLFENCHL SVSDAVGTITAQRRESPSE TGF FMGCKI+GINSAVLGRPWG
Sbjct: 181 DFICGNAASLFENCHLHSVSDAVGTITAQRRESPSETTGFSFMGCKITGINSAVLGRPWG 240

Query: 241 AFSRVVFGYTYMSGVILPEGWDDWQDPSKHSTVYYGQYRCYGKGANPSRRVPWSFTNMTS 300
           A+SRVVFGYTYMS +ILP+GWDDWQDPSKHSTVYYGQYRCYGKGANPSRRVPWS TNMTS
Sbjct: 241 AYSRVVFGYTYMSDLILPQGWDDWQDPSKHSTVYYGQYRCYGKGANPSRRVPWSLTNMTS 300

Query: 301 QEAAPFFTKTFIGAADWLRPLPTTFKRTFSSKP 329
           QEAAPFF K+FIGAADWLRPLPTTFKR FSSKP
Sbjct: 301 QEAAPFFNKSFIGAADWLRPLPTTFKRAFSSKP 333

BLAST of Lag0004394 vs. ExPASy TrEMBL
Match: A0A1S3BBW3 (Pectinesterase OS=Cucumis melo OX=3656 GN=LOC103487980 PE=4 SV=1)

HSP 1 Score: 570.5 bits (1469), Expect = 4.6e-159
Identity = 280/328 (85.37%), Postives = 301/328 (91.77%), Query Frame = 0

Query: 1   MSRRNGFSLVFYTAIAICVLVAEVAAA-TTAILITVDQSGKGNFTKIQDAIDAVPENNSE 60
           MSRR   S +   ++   VLVAEV AA TTA+LITVDQSGKGNFTKIQ AIDAVPENN E
Sbjct: 1   MSRR---SFLVVVSVIAAVLVAEVTAAETTAVLITVDQSGKGNFTKIQQAIDAVPENNRE 60

Query: 61  EVFIAVKPGIYREKVIVPANKPYITISGRKAVDTIITWNDSKNTYNSATLAVLASDFVGR 120
           EVFI VK GIYREKV+VPANKP+ITISGR+A DTII+WNDSKNTYNSATLAVLASDFVGR
Sbjct: 61  EVFIVVKAGIYREKVVVPANKPFITISGRRAADTIISWNDSKNTYNSATLAVLASDFVGR 120

Query: 121 YLTIQNGFGAGAQAVALRVSGDRVSFVACRFVGHQDTLLDDIGRHYYKSCYIEGATDFIC 180
           YLTIQN +GAGAQAVALRVSGDRVSF ACRF+GHQDTLLDDIGRHYYKSCYI+GATDFIC
Sbjct: 121 YLTIQNEYGAGAQAVALRVSGDRVSFTACRFLGHQDTLLDDIGRHYYKSCYIQGATDFIC 180

Query: 181 GNGASLFENCHLRSVSDAVGTITAQRRESPSENTGFVFMGCKISGINSAVLGRPWGAFSR 240
           GN ASLFENCHLRSVSD VGTITAQRRESPSENTGFVF+GCKI+GINSAVLGRPWGAFSR
Sbjct: 181 GNAASLFENCHLRSVSDDVGTITAQRRESPSENTGFVFLGCKITGINSAVLGRPWGAFSR 240

Query: 241 VVFGYTYMSGVILPEGWDDWQDPSKHSTVYYGQYRCYGKGANPSRRVPWSFTNMTSQEAA 300
           VVFG+T+MS VILPEGWD+WQDPSK STVYYGQY+CYGKGANPSRRV WSFTNMT+Q+AA
Sbjct: 241 VVFGFTFMSDVILPEGWDNWQDPSKQSTVYYGQYKCYGKGANPSRRVSWSFTNMTAQDAA 300

Query: 301 PFFTKTFIGAADWLRPLPTTFKRTFSSK 328
           PFFTK+FIGAADWLRPLP  FKR FSS+
Sbjct: 301 PFFTKSFIGAADWLRPLPKHFKRAFSSR 325

BLAST of Lag0004394 vs. ExPASy TrEMBL
Match: A0A0A0LPB4 (Pectinesterase OS=Cucumis sativus OX=3659 GN=Csa_2G361760 PE=4 SV=1)

HSP 1 Score: 565.5 bits (1456), Expect = 1.5e-157
Identity = 275/323 (85.14%), Postives = 297/323 (91.95%), Query Frame = 0

Query: 8   SLVFYTAIAICVLVAEV--AAATTAILITVDQSGKGNFTKIQDAIDAVPENNSEEVFIAV 67
           S +   ++   VLVAEV  AA TTA+LITVDQSGKGNFTKIQ AIDAVP NN EEVFI+V
Sbjct: 5   SFLLAISVIAAVLVAEVTAAAVTTAVLITVDQSGKGNFTKIQQAIDAVPINNKEEVFISV 64

Query: 68  KPGIYREKVIVPANKPYITISGRKAVDTIITWNDSKNTYNSATLAVLASDFVGRYLTIQN 127
           K GIYREKV+VPANKP+ITISGR+AVDTII+WNDSKNTYNSATLAVLASDFVGRYLTIQN
Sbjct: 65  KAGIYREKVVVPANKPFITISGRRAVDTIISWNDSKNTYNSATLAVLASDFVGRYLTIQN 124

Query: 128 GFGAGAQAVALRVSGDRVSFVACRFVGHQDTLLDDIGRHYYKSCYIEGATDFICGNGASL 187
           G+G GAQAVALRVSGDRVSF ACRF+GHQDTLLDDIGRHYYKSCYI+GATDFICGN ASL
Sbjct: 125 GYGPGAQAVALRVSGDRVSFTACRFLGHQDTLLDDIGRHYYKSCYIQGATDFICGNAASL 184

Query: 188 FENCHLRSVSDAVGTITAQRRESPSENTGFVFMGCKISGINSAVLGRPWGAFSRVVFGYT 247
           FENCHLRSVS+ VGTITAQRRESPSENTGFVFMGCKI+GINSAVLGRPWGAFSRVVFG+T
Sbjct: 185 FENCHLRSVSEDVGTITAQRRESPSENTGFVFMGCKITGINSAVLGRPWGAFSRVVFGFT 244

Query: 248 YMSGVILPEGWDDWQDPSKHSTVYYGQYRCYGKGANPSRRVPWSFTNMTSQEAAPFFTKT 307
           +MS VILPEGWD+WQDPSK STVYYGQY+CYGKGAN SRRV WSFTNMT+Q+AAPFFTK+
Sbjct: 245 FMSDVILPEGWDNWQDPSKQSTVYYGQYKCYGKGANTSRRVSWSFTNMTAQDAAPFFTKS 304

Query: 308 FIGAADWLRPLPTTFKRTFSSKP 329
           FIGAADWLRP+P  FKR FSS P
Sbjct: 305 FIGAADWLRPVPNRFKRAFSSHP 327

BLAST of Lag0004394 vs. TAIR 10
Match: AT2G21610.1 (pectinesterase 11 )

HSP 1 Score: 368.2 bits (944), Expect = 6.7e-102
Identity = 170/296 (57.43%), Postives = 223/296 (75.34%), Query Frame = 0

Query: 28  TTAILITVDQSGKGNFTKIQDAIDAVPE--NNSEEVFIAVKPGIYREKVIVPANKPYITI 87
           +TAILI VDQSGKG+F+KIQ+AI+++P   NNS+  FI VKPGIYREKV++PA KPYIT+
Sbjct: 46  STAILIRVDQSGKGDFSKIQEAIESIPPNLNNSQLYFIWVKPGIYREKVVIPAEKPYITL 105

Query: 88  SGRKAVDTIITWNDSKNTYNSATLAVLASDFVGRYLTIQNGFGAGAQAVALRVSGDRVSF 147
           SG +A +T + W+D ++   S TL + ASDFV R+LTIQN FG   +AVALRV+ D+ +F
Sbjct: 106 SGTQASNTFLIWSDGEDILESPTLTIFASDFVCRFLTIQNKFGTAGRAVALRVAADKAAF 165

Query: 148 VACRFVGHQDTLLDDIGRHYYKSCYIEGATDFICGNGASLFENCHLRSVSDAVGTITAQR 207
             C    +QDTLLDD G HY+K+CYIEGATDFICG+ +SL+E CHL S+S   G+ITAQ 
Sbjct: 166 YGCVITSYQDTLLDDNGNHYFKNCYIEGATDFICGSASSLYERCHLHSLSPNNGSITAQM 225

Query: 208 RESPSENTGFVFMGCKISGINSAVLGRPWGAFSRVVFGYTYMSGVILPEGWDDWQDPSKH 267
           R S +E +GF F+GCK++G  S  LGRPWGA+SRVVF Y++ S V+ P+GW+ W D +K 
Sbjct: 226 RTSATEKSGFTFLGCKLTGSGSTFLGRPWGAYSRVVFAYSFFSNVVAPQGWNQWGDSTKE 285

Query: 268 STVYYGQYRCYGKGANPSRRVPWSFTNMTSQEAAPFFTKTFIGAADWLRPLPTTFK 322
           +TVYYG+Y+CYG GA+  +RV WS   ++ +EA  F +K FIG  DWLRP P+ FK
Sbjct: 286 NTVYYGEYKCYGPGADREQRVEWS-KQLSDEEATVFLSKDFIGGKDWLRPAPSHFK 340

BLAST of Lag0004394 vs. TAIR 10
Match: AT5G19730.1 (Pectin lyase-like superfamily protein )

HSP 1 Score: 296.2 bits (757), Expect = 3.2e-80
Identity = 145/294 (49.32%), Postives = 188/294 (63.95%), Query Frame = 0

Query: 37  QSGKGNFTKIQDAIDAVPENNSEEVFIAVKPGIYREKVIVPANKPYITISGRKAVDTIIT 96
           +S KG+FTKIQDAID++P  N   V I V  G+Y+EKV +P  K +ITI G  A  T + 
Sbjct: 90  KSNKGDFTKIQDAIDSLPLINFVRVVIKVHAGVYKEKVSIPPLKAFITIEGEGAEKTTVE 149

Query: 97  WNDS----------KNTYNSATLAVLASDFVGRYLTIQNGF------GAGAQAVALRVSG 156
           W D+            TYNSA+ AV +  FV + +T +N          G QAVALRVS 
Sbjct: 150 WGDTAQTPDSKGNPMGTYNSASFAVNSPFFVAKNITFRNTTPVPLPGAVGKQAVALRVSA 209

Query: 157 DRVSFVACRFVGHQDTLLDDIGRHYYKSCYIEGATDFICGNGASLFENCHLRSVSDAVGT 216
           D  +F  CR +G QDTL D +GRHYYK CYIEG+ DFI GN  SL+E CH+ +++D +G 
Sbjct: 210 DNAAFFGCRMLGAQDTLYDHLGRHYYKDCYIEGSVDFIFGNALSLYEGCHVHAIADKLGA 269

Query: 217 ITAQRRESPSENTGFVFMGCKISGINSAVLGRPWGAFSRVVFGYTYMSGVILPEGWDDWQ 276
           +TAQ R S  E+TGF F+ CK++G     LGR WG FSRVVF YTYM  +ILP GW +W 
Sbjct: 270 VTAQGRSSVLEDTGFSFVKCKVTGTGVLYLGRAWGPFSRVVFAYTYMDNIILPRGWYNWG 329

Query: 277 DPSKHSTVYYGQYRCYGKGANPSRRVPWSFTNMTSQEAAPFFTKTFIGAADWLR 315
           DPS+  TV+YGQY+C G GAN   RV W+   +T +EA PF + TFI  ++W++
Sbjct: 330 DPSREMTVFYGQYKCTGAGANYGGRVAWA-RELTDEEAKPFLSLTFIDGSEWIK 382

BLAST of Lag0004394 vs. TAIR 10
Match: AT2G36710.1 (Pectin lyase-like superfamily protein )

HSP 1 Score: 267.7 bits (683), Expect = 1.2e-71
Identity = 136/301 (45.18%), Postives = 189/301 (62.79%), Query Frame = 0

Query: 29  TAILITVDQSGKGNFTKIQDAIDAVPENNSEEVFIAVKPGIYREKVIVPANKPYITISGR 88
           T++++TVD  G GNF+ +Q AID VP+ +S +  I V  G YREKV V  NK  + I GR
Sbjct: 87  TSLVLTVDLHGCGNFSNVQSAIDVVPDLSSSKTLIIVNSGSYREKVTVNENKTNLVIQGR 146

Query: 89  KAVDTIITWNDSK----NTYNSATLAVLASDFVGRYLTIQNGF------GAGAQAVALRV 148
              +T I WND+     NT +S +  V A++F    ++ +N         A AQAVALR+
Sbjct: 147 GYQNTSIEWNDTAKSAGNTADSFSFVVFAANFTAYNISFKNNAPEPDPGEADAQAVALRI 206

Query: 149 SGDRVSFVACRFVGHQDTLLDDIGRHYYKSCYIEGATDFICGNGASLFENCHLRSV---- 208
            GD+ +F  C F G QDTLLDD GRH++K C+I+G+ DFI GNG SL+++C + S+    
Sbjct: 207 EGDQAAFYGCGFYGAQDTLLDDKGRHFFKECFIQGSIDFIFGNGRSLYQDCTINSIAKGN 266

Query: 209 -SDAVGTITAQRRESPSENTGFVFMGCKISGINSAVLGRPWGAFSRVVFGYTYMSGVILP 268
            S   G+ITAQ R+S  E +GF F+ CKI G    +LGR WGA++ VVF  TYMSG+I P
Sbjct: 267 TSGVTGSITAQGRQSEDEQSGFSFVNCKIDGSGEILLGRAWGAYATVVFSNTYMSGIITP 326

Query: 269 EGWDDWQDPSKHSTVYYGQYRCYGKGANPSRRVPWSFTNMTSQEAAPFFTKTFIGAADWL 315
           EGW++W D +K  TV +G+++CYG GA+   RV +    +T  EA+ F   +FI   +WL
Sbjct: 327 EGWNNWGDSTKEKTVTFGEHKCYGPGADYKERVLFG-KQLTDSEASSFIDVSFIDGDEWL 386

BLAST of Lag0004394 vs. TAIR 10
Match: AT5G47500.1 (Pectin lyase-like superfamily protein )

HSP 1 Score: 266.2 bits (679), Expect = 3.6e-71
Identity = 128/301 (42.52%), Postives = 178/301 (59.14%), Query Frame = 0

Query: 32  LITVDQSGKGNFTKIQDAIDAVPENNSEEVFIAVKPGIYREKVIVPANKPYITISGRKAV 91
           +ITV  +G   F  +QDA+D++P+NN++ + I + PG YREKV+VPA KPYIT  G    
Sbjct: 60  VITVSLNGHAQFRSVQDAVDSIPKNNNKSITIKIAPGFYREKVVVPATKPYITFKGAGRD 119

Query: 92  DTIITWNDSKN----------TYNSATLAVLASDFVGRYLTIQNGFGA------GAQAVA 151
            T I W+D  +          TY +A++ V A+ F  R ++  N   A      G QAVA
Sbjct: 120 VTAIEWHDRASDLGANGQQLRTYQTASVTVYANYFTARNISFTNTAPAPLPGMQGWQAVA 179

Query: 152 LRVSGDRVSFVACRFVGHQDTLLDDIGRHYYKSCYIEGATDFICGNGASLFENCHLRSVS 211
            R+SGD+  F  C F G QDTL DD GRHY+K CYIEG+ DFI GNG S++++C L S++
Sbjct: 180 FRISGDKAFFSGCGFYGAQDTLCDDAGRHYFKECYIEGSIDFIFGNGRSMYKDCELHSIA 239

Query: 212 DAVGTITAQRRESPSENTGFVFMGCKISGINSAVLGRPWGAFSRVVFGYTYMSGVILPEG 271
              G+I A  R  P E TGF F+GC+++G     +GR  G +SR+V+ YTY   ++   G
Sbjct: 240 SRFGSIAAHGRTCPEEKTGFAFVGCRVTGTGPLYVGRAMGQYSRIVYAYTYFDALVAHGG 299

Query: 272 WDDWQDPS-KHSTVYYGQYRCYGKGANPSRRVPWSFTNMTSQEAAPFFTKTFIGAADWLR 316
           WDDW   S K  T ++G Y CYG GA  +R V W+   +  + A PF  K+F+    W+ 
Sbjct: 300 WDDWDHKSNKSKTAFFGVYNCYGPGAAATRGVSWA-RALDYESAHPFIAKSFVNGRHWIA 359

BLAST of Lag0004394 vs. TAIR 10
Match: AT5G55590.1 (Pectin lyase-like superfamily protein )

HSP 1 Score: 255.8 bits (652), Expect = 4.9e-68
Identity = 130/310 (41.94%), Postives = 182/310 (58.71%), Query Frame = 0

Query: 22  AEVAAATTAILITVDQSGKGNFTKIQDAIDAVPENNSEEVFIAVKPGIYREKVIVPANKP 81
           A   A     +I VD++G G+   +Q A+D VP++NS+ V I + PGIYREKVIVP +KP
Sbjct: 71  ANANATNVRRVIVVDKNGGGDSVTVQGAVDMVPDSNSQRVKIFILPGIYREKVIVPKSKP 130

Query: 82  YITISGRK--AVDTIITWNDSKN----------TYNSATLAVLASDFVGRYLTIQNGFGA 141
           YI+  G +  A DT+I+W+D  +          TY +A++++ +  F    +T +N   A
Sbjct: 131 YISFIGNESYAGDTVISWSDKASDLGCDGKELGTYRTASVSIESDFFCATAITFENTVVA 190

Query: 142 -----GAQAVALRVSGDRVSFVACRFVGHQDTLLDDIGRHYYKSCYIEGATDFICGNGAS 201
                G QAVALR+ GD+  F   R +G QDTL DD G HY+  CYI+G  DFI GN  S
Sbjct: 191 EAGEQGRQAVALRIIGDKAVFYRVRVLGSQDTLFDDNGSHYFYQCYIQGNVDFIFGNAKS 250

Query: 202 LFENCHLRSVSDAVGTITAQRRESPSENTGFVFMGCKISGINSAVLGRPWGAFSRVVFGY 261
           L+++C + S +   G I A  R+S +E+TGF F+ C ISG     LGR WG +SR V+  
Sbjct: 251 LYQDCDIHSTAKRYGAIAAHHRDSETEDTGFSFVNCDISGTGQIYLGRAWGNYSRTVYSN 310

Query: 262 TYMSGVILPEGWDDWQDPSKHSTVYYGQYRCYGKGANPSRRVPWSFTNMTSQEAAPFFTK 315
            +++ +I P GW DW+ P +   V +G+Y C G+GA    RVPWS T +T  E  PF  +
Sbjct: 311 CFIADIITPVGWSDWKHPERQRKVMFGEYNCRGRGAERGGRVPWSKT-LTRDEVKPFLGR 370

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
XP_023536774.14.7e-16689.09putative pectinesterase 11 [Cucurbita pepo subsp. pepo][more]
XP_022951672.16.2e-16688.79putative pectinesterase 11 [Cucurbita moschata][more]
KAG6585588.11.8e-16588.48putative pectinesterase 11, partial [Cucurbita argyrosperma subsp. sororia] >KAG... [more]
XP_023002424.11.8e-16588.48putative pectinesterase 11 [Cucurbita maxima][more]
XP_022144190.12.9e-16386.19putative pectinesterase 11 [Momordica charantia][more]
Match NameE-valueIdentityDescription
Q9SIJ99.4e-10157.43Putative pectinesterase 11 OS=Arabidopsis thaliana OX=3702 GN=PME11 PE=3 SV=1[more]
Q8VYZ34.6e-7949.32Probable pectinesterase 53 OS=Arabidopsis thaliana OX=3702 GN=PME53 PE=2 SV=1[more]
Q9ZQA31.7e-7045.18Probable pectinesterase 15 OS=Arabidopsis thaliana OX=3702 GN=PME15 PE=2 SV=1[more]
Q8LPF35.1e-7042.52Probable pectinesterase 68 OS=Arabidopsis thaliana OX=3702 GN=PME68 PE=2 SV=1[more]
Q9FM796.8e-6741.94Pectinesterase QRT1 OS=Arabidopsis thaliana OX=3702 GN=QRT1 PE=2 SV=1[more]
Match NameE-valueIdentityDescription
A0A6J1GIB53.0e-16688.79Pectinesterase OS=Cucurbita moschata OX=3662 GN=LOC111454415 PE=4 SV=1[more]
A0A6J1KQF08.7e-16688.48Pectinesterase OS=Cucurbita maxima OX=3661 GN=LOC111496269 PE=4 SV=1[more]
A0A6J1CQY51.4e-16386.19Pectinesterase OS=Momordica charantia OX=3673 GN=LOC111013943 PE=4 SV=1[more]
A0A1S3BBW34.6e-15985.37Pectinesterase OS=Cucumis melo OX=3656 GN=LOC103487980 PE=4 SV=1[more]
A0A0A0LPB41.5e-15785.14Pectinesterase OS=Cucumis sativus OX=3659 GN=Csa_2G361760 PE=4 SV=1[more]
Match NameE-valueIdentityDescription
AT2G21610.16.7e-10257.43pectinesterase 11 [more]
AT5G19730.13.2e-8049.32Pectin lyase-like superfamily protein [more]
AT2G36710.11.2e-7145.18Pectin lyase-like superfamily protein [more]
AT5G47500.13.6e-7142.52Pectin lyase-like superfamily protein [more]
AT5G55590.14.9e-6841.94Pectin lyase-like superfamily protein [more]
InterPro
Analysis Name: InterPro Annotations of Sponge gourd (AG-4) v1
Date Performed: 2022-08-01
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR000070Pectinesterase, catalyticPFAMPF01095Pectinesterasecoord: 33..308
e-value: 2.4E-73
score: 246.4
IPR012334Pectin lyase foldGENE3D2.160.20.10coord: 27..323
e-value: 2.1E-109
score: 367.8
NoneNo IPR availablePANTHERPTHR31321:SF77PECTINESTERASE 11-RELATEDcoord: 10..314
NoneNo IPR availablePANTHERPTHR31321ACYL-COA THIOESTER HYDROLASE YBHC-RELATEDcoord: 10..314
IPR011050Pectin lyase fold/virulence factorSUPERFAMILY51126Pectin lyase-likecoord: 27..321

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Lag0004394.1Lag0004394.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0042545 cell wall modification
biological_process GO:0045490 pectin catabolic process
cellular_component GO:0005576 extracellular region
cellular_component GO:0016021 integral component of membrane
molecular_function GO:0045330 aspartyl esterase activity
molecular_function GO:0030599 pectinesterase activity