Lag0004365 (gene) Sponge gourd (AG‐4) v1

BLAST of Lag0004365 vs. NCBI nr
Match: XP_022144258.1 (uncharacterized membrane protein At1g75140-like [Momordica charantia])

HSP 1 Score: 1159.1 bits (2997), Expect = 0.0e+00
Identity = 589/657 (89.65%), Postives = 610/657 (92.85%), Query Frame = 0

Query: 1   MANPLKGKLLLIFFLFVFAIPHVSRVFVCSSSSSEQNEP----EPEVPFEIRQYDLLKNL 60
           MANP KGKLLLI+FLFVF +PHVSRVFVCSSSS EQN+P    E EVPFEIRQ DLLKNL
Sbjct: 1   MANPRKGKLLLIYFLFVFGVPHVSRVFVCSSSSFEQNQPESESESEVPFEIRQRDLLKNL 60

Query: 61  EELVRNLSDVVSKLELRLSDIPTAVHRDKKLNTDALRRVVPEVVGSGGGKEDGISDGGDG 120
           EELVRNLSDVVSKLE+RLSDIPT V RDKK NTDA RRVVPEVV  GG KEDG++DGGDG
Sbjct: 61  EELVRNLSDVVSKLEMRLSDIPTEVIRDKKPNTDASRRVVPEVVSLGGRKEDGLNDGGDG 120

Query: 121 GLDNEIQDGGRARAVSVTKYSPFWSERFHFLSAVKLDSDATCINVLPLRDFEGHSKFVAV 180
           GL+++I+DG R RAVSVTKYSPFWSERFHFLSAVKLDSDATCINVLP RDFEGHSK+V V
Sbjct: 121 GLESKIRDGDRTRAVSVTKYSPFWSERFHFLSAVKLDSDATCINVLPFRDFEGHSKYVVV 180

Query: 181 GDERGRVYVFTRNGDVAFELRTMSESPITAMLSYMSIYKNETLLVTGHKNGAILMHRIWE 240
           GDE+GRVYVFTRNGDVA ELRT SESPI  MLSYMSIYKNETLLVTGHKNGAILMHRIWE
Sbjct: 181 GDEKGRVYVFTRNGDVAVELRTKSESPIMTMLSYMSIYKNETLLVTGHKNGAILMHRIWE 240

Query: 241 GSNGEELNSLFMEHVVEFVATDGREDESPISTLEVHHVGRTRYILSSDARGKIKVFRENG 300
            SNGEE +SLFMEHVVEFVATDGREDESPIS LEVHHVGRTRYILSSD RGKIKVFRENG
Sbjct: 241 RSNGEESHSLFMEHVVEFVATDGREDESPISILEVHHVGRTRYILSSDVRGKIKVFRENG 300

Query: 301 TVYGSVMATSRPIAFLKQRLLFLTESGAGSLDLRSMKLRESECEGLNYSLARNYVFDATE 360
           TVYGSVM  S+PIAFLKQRLLFLTESGAGSLDLRSMKLRESECEGLNYSLARNYVFDATE
Sbjct: 301 TVYGSVMPESKPIAFLKQRLLFLTESGAGSLDLRSMKLRESECEGLNYSLARNYVFDATE 360

Query: 361 RSKAYGLTSDGDLIHVLLLGDIMNFKCRVRSKRKFEFDEPLGFQAIKGYLLVISNEKVHI 420
           RSKAYG TS GDLIHVLLLGDIMNFKCRVRSKRKFE DEPL FQAIKGYLLVISNEKVHI
Sbjct: 361 RSKAYGFTSTGDLIHVLLLGDIMNFKCRVRSKRKFELDEPLVFQAIKGYLLVISNEKVHI 420

Query: 421 FNVSSQHYVRVGAPRHLFSAGLDEIRSSFLNNQNLDLDSEGKFTPLISSDREKLVVIGLG 480
           FNVSSQHY RVGAPR LFSA +DEIRSSFLN QNLDL+SEGKF PLISSDREKLVV+GLG
Sbjct: 421 FNVSSQHYARVGAPRPLFSAAVDEIRSSFLNYQNLDLESEGKFVPLISSDREKLVVLGLG 480

Query: 481 GGYVGMYRSNLPIFKGEFNTMLWTSPVLFFILFLFGAWQFFAKKKEALTSWGPDDPFTAT 540
           GGYVGMYRSNLPIFKGEFNTMLWTSPVLFFILFLFGAW FFAKKKEALTSWGPDDPFTAT
Sbjct: 481 GGYVGMYRSNLPIFKGEFNTMLWTSPVLFFILFLFGAWHFFAKKKEALTSWGPDDPFTAT 540

Query: 541 SPTTGAPLVTGSSERASFIDTASRSTDVMDLRGGSGLRGPPRRYGSPTGYPGGATSSFRP 600
           SPTTGAPL TG+SER+SFIDT SRSTD+MDLRGGSGLRGPPRRYGSPTGYPGGAT+SFRP
Sbjct: 541 SPTTGAPLGTGTSERSSFIDTPSRSTDMMDLRGGSGLRGPPRRYGSPTGYPGGATNSFRP 600

Query: 601 ATTSDHNSRPAAVDPNYRAASELKFRGSPLEPPGFPKRREPLFANNQVVNQVVDESS 654
            TTSDHNSRPAAVDPNYRAASELKFRGSPLEPPGFPKRREPLFAN    NQVVDESS
Sbjct: 601 PTTSDHNSRPAAVDPNYRAASELKFRGSPLEPPGFPKRREPLFAN----NQVVDESS 653

BLAST of Lag0004365 vs. NCBI nr
Match: XP_038885478.1 (uncharacterized membrane protein At1g75140-like [Benincasa hispida])

HSP 1 Score: 1153.3 bits (2982), Expect = 0.0e+00
Identity = 583/657 (88.74%), Postives = 613/657 (93.30%), Query Frame = 0

Query: 1   MANPLKGKLLLIFFLFVFAIPHVSRVFVCSSSSSEQNEPEPEVPFEIRQYDLLKNLEELV 60
           MAN  KGKLL I+FLFVF+ PHVSRVFVCSSSS E N+ E EVPFEI+QY+LLKNLEELV
Sbjct: 1   MANLRKGKLLFIYFLFVFSFPHVSRVFVCSSSSFELNQSEIEVPFEIKQYNLLKNLEELV 60

Query: 61  RNLSDVVSKLELRLSDIPTAVHRDKKLNTDALRRVVPEVVGSGG----GKEDGISDGGDG 120
           RNLSDVVSKLELRLSDIPTAVH+D KLNTDA RRVV EVVG GG    GKEDG++DGGDG
Sbjct: 61  RNLSDVVSKLELRLSDIPTAVHKD-KLNTDASRRVVSEVVGLGGVKRKGKEDGLNDGGDG 120

Query: 121 GLDNEIQDGGRARAVSVTKYSPFWSERFHFLSAVKLDSDATCINVLPLRDFEGHSKFVAV 180
           GLDN+IQDGGR RAVSVTKYSPFWSERFHFLSAVKL+SDATCINVLP+RDFEGHSK+VAV
Sbjct: 121 GLDNKIQDGGRMRAVSVTKYSPFWSERFHFLSAVKLESDATCINVLPIRDFEGHSKYVAV 180

Query: 181 GDERGRVYVFTRNGDVAFELRTMSESPITAMLSYMSIYKNETLLVTGHKNGAILMHRIWE 240
           GDE+GR+YVF RNGDVA EL T+SESPITAMLSY SIYKNETLLVTGH NGAILMHRIWE
Sbjct: 181 GDEKGRIYVFVRNGDVAIELPTVSESPITAMLSYTSIYKNETLLVTGHNNGAILMHRIWE 240

Query: 241 GSNGEELNSLFMEHVVEFVATDGREDESPISTLEVHHVGRTRYILSSDARGKIKVFRENG 300
           G NGEELNS+FMEHVVEFVATD RED+SPIS LEVHHVGRTRYILSSD RGKIKVF+E+G
Sbjct: 241 GLNGEELNSIFMEHVVEFVATDSREDDSPISILEVHHVGRTRYILSSDFRGKIKVFKEDG 300

Query: 301 TVYGSVMATSRPIAFLKQRLLFLTESGAGSLDLRSMKLRESECEGLNYSLARNYVFDATE 360
           TVYGSV   SRPIAFLKQRLLFLTESGAGSLDLRSMKLRE+ CEGLNYSLARNYVFDATE
Sbjct: 301 TVYGSVTLMSRPIAFLKQRLLFLTESGAGSLDLRSMKLREAGCEGLNYSLARNYVFDATE 360

Query: 361 RSKAYGLTSDGDLIHVLLLGDIMNFKCRVRSKRKFEFDEPLGFQAIKGYLLVISNEKVHI 420
           RSKAYG TSDGDLIHVLLLGDIMNFKCRVRSKRKFE DEPL FQAIKGYLLVISNEKVH+
Sbjct: 361 RSKAYGFTSDGDLIHVLLLGDIMNFKCRVRSKRKFELDEPLVFQAIKGYLLVISNEKVHV 420

Query: 421 FNVSSQHYVRVGAPRHLFSAGLDEIRSSFLNNQNLDLDSEGKFTPLISSDREKLVVIGLG 480
           FNVSSQHYVRV APR LFSAGLDEIRSSFLN QNL+L+SEGKF PLISSD EKLVV+GLG
Sbjct: 421 FNVSSQHYVRVAAPRLLFSAGLDEIRSSFLNYQNLNLESEGKFIPLISSDHEKLVVLGLG 480

Query: 481 GGYVGMYRSNLPIFKGEFNTMLWTSPVLFFILFLFGAWQFFAKKKEALTSWGPDDPFTAT 540
           GGYVGMYRSNLPIFKGEFN+MLWTSPVLFFILFLFGAWQFFAKKKEALTSWGPDDPFTAT
Sbjct: 481 GGYVGMYRSNLPIFKGEFNSMLWTSPVLFFILFLFGAWQFFAKKKEALTSWGPDDPFTAT 540

Query: 541 SPTTGAPLVTGSSERASFIDTASRSTDVMDLRGGSGLRGPPRRYGSPTGYPGGATSSFRP 600
           SPTTG PL TGSSERASFIDT SRSTD+MDLRGG G+RGPPR YGSPTGYPGGATSSFRP
Sbjct: 541 SPTTGVPLGTGSSERASFIDTPSRSTDMMDLRGGGGIRGPPRHYGSPTGYPGGATSSFRP 600

Query: 601 ATTSDHNSRPAAVDPNYRAASELKFRGSPLEPPGFPKRREPLFANNQVVNQVVDESS 654
            TTSDH+SRPAAVDP+YRAASELKFRGSPLEPPGFPKRREPLFANNQVVNQ+VDESS
Sbjct: 601 PTTSDHSSRPAAVDPSYRAASELKFRGSPLEPPGFPKRREPLFANNQVVNQMVDESS 656

BLAST of Lag0004365 vs. NCBI nr
Match: XP_022996718.1 (uncharacterized membrane protein At1g75140-like [Cucurbita maxima])

HSP 1 Score: 1148.7 bits (2970), Expect = 0.0e+00
Identity = 580/667 (86.96%), Postives = 611/667 (91.60%), Query Frame = 0

Query: 1   MANPLKGKLLLIFFLFVFAIPHVSRVFVCSSSSSEQNEPEPEVPFEIRQYDLLKNLEELV 60
           MANP KGKLL I+FLFVF IPHVSR FVC SSS E N+ E EVPFE RQYDLLKNLEELV
Sbjct: 1   MANPRKGKLLFIYFLFVFGIPHVSRDFVCCSSSFELNQSETEVPFETRQYDLLKNLEELV 60

Query: 61  RNLSDVVSKLELRLSDIPTAVHRDKKLNTDALRRVVPEVVGSGGGKEDGISDGGDGGLDN 120
           RNLSDVVSKLELRLS+IPTAVHRD KLNTDA RRVVPEVV  GGGKEDG++DGGDG LD 
Sbjct: 61  RNLSDVVSKLELRLSNIPTAVHRDVKLNTDASRRVVPEVVSLGGGKEDGLNDGGDGALDT 120

Query: 121 EIQDGGRARAVSVTKYSPFWSERFHFLSAVKLDSDATCINVLPLRDFEGHSKFVAVGDER 180
           +IQDGGR RAVSVTKYSPFWSERFHFLSAVKL+SDAT INVLPLRDFEGHSK+VAVGDE 
Sbjct: 121 KIQDGGRGRAVSVTKYSPFWSERFHFLSAVKLESDATSINVLPLRDFEGHSKYVAVGDET 180

Query: 181 GRVYVFTRNGDVAFELRTMSESPITAMLSYMSIYKNETLLVTGHKNGAILMHRIWEGSNG 240
           GR+YVF RNGDVA ELRTMS SPITA LSYMSIYKNETLLVTGH++GAI M RIWEGSNG
Sbjct: 181 GRIYVFVRNGDVAVELRTMSGSPITATLSYMSIYKNETLLVTGHESGAISMLRIWEGSNG 240

Query: 241 EELNSLFMEHVVEFVATDGREDESPISTLEVHHVGRTRYILSSDARGKIKVFRENGTVYG 300
           EE NS+FMEHV+EFV TDGRE++SPIS LEVHHVGRTRYILSSD +GKIKVFRE+GTVYG
Sbjct: 241 EESNSIFMEHVLEFVVTDGREEKSPISILEVHHVGRTRYILSSDVKGKIKVFREDGTVYG 300

Query: 301 SVMATSRPIAFLKQRLLFLTESGAGSLDLRSMKLRESECEGLNYSLARNYVFDATERSKA 360
           SVM TSRPIAFLKQRLLFLTE+GAGSLDLRSMKLRESECEGLNYSLARNYVFDATERSKA
Sbjct: 301 SVMPTSRPIAFLKQRLLFLTETGAGSLDLRSMKLRESECEGLNYSLARNYVFDATERSKA 360

Query: 361 YGLTSDGDLIHVLLLGDIMNFKCRVRSKRKFEFDEPLGFQAIKGYLLVISNEKVHIFNVS 420
           YG TSDGDLIHV+LLGDIMNFKCRVRSK+KFE DEPL FQA+KGYLLVI +EKVH+FNVS
Sbjct: 361 YGFTSDGDLIHVMLLGDIMNFKCRVRSKKKFELDEPLFFQAVKGYLLVIGSEKVHVFNVS 420

Query: 421 SQHYVRVGAPRHLFSAGLDEIRSSFLNNQNLDLDSEGKFTPLISSDREKLVVIGLGGGYV 480
           SQHYVRVGAPR LFSA LDEIRSSFLNNQNLDL+S+GKF PLISSDREKLVV+GLGGGY+
Sbjct: 421 SQHYVRVGAPRLLFSARLDEIRSSFLNNQNLDLESQGKFIPLISSDREKLVVLGLGGGYI 480

Query: 481 GMYRSNLPIFKGEFNTMLWTSPVLFFILFLFGAWQFFAKKKEALTSWGPDDPFTATSPTT 540
           GMYRSNLPI+KGEFNTMLWTSPVLFFILFLFGAWQFFAKKKEALTSWGPDDPFTATSPTT
Sbjct: 481 GMYRSNLPIYKGEFNTMLWTSPVLFFILFLFGAWQFFAKKKEALTSWGPDDPFTATSPTT 540

Query: 541 GAPLVTGSSE--------------RASFIDTASRSTDVMDLRGGSGLRGPPRRYGSPTGY 600
           GAPL TGS+E              R SFIDT SRSTD+MDLRGGSGLRGPPRRYGSPTGY
Sbjct: 541 GAPLGTGSTERTSFIDTPSRSTDMRTSFIDTPSRSTDMMDLRGGSGLRGPPRRYGSPTGY 600

Query: 601 PGGATSSFRPATTSDHNSRPAAVDPNYRAASELKFRGSPLEPPGFPKRREPLFANNQVVN 654
           PGG TSSFRPATTSDH+SR AAVDPNYRAASELKFRGSPLEPPGFPKRREPLFANNQVVN
Sbjct: 601 PGGVTSSFRPATTSDHSSRQAAVDPNYRAASELKFRGSPLEPPGFPKRREPLFANNQVVN 660

BLAST of Lag0004365 vs. NCBI nr
Match: XP_023546241.1 (uncharacterized membrane protein At1g75140-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 1146.3 bits (2964), Expect = 0.0e+00
Identity = 577/667 (86.51%), Postives = 611/667 (91.60%), Query Frame = 0

Query: 1   MANPLKGKLLLIFFLFVFAIPHVSRVFVCSSSSSEQNEPEPEVPFEIRQYDLLKNLEELV 60
           MANP KGKLL I+FLFVF IP+VSR FVC SSS E N+ E EVPFE RQYD+LKNLEELV
Sbjct: 1   MANPRKGKLLFIYFLFVFGIPYVSRDFVCCSSSLELNQSETEVPFETRQYDVLKNLEELV 60

Query: 61  RNLSDVVSKLELRLSDIPTAVHRDKKLNTDALRRVVPEVVGSGGGKEDGISDGGDGGLDN 120
           RNLSDVVSKLELRLS+IPTAVHRD KLNTDA RRVVPEVV  GGGK+DG++DGGDG LD 
Sbjct: 61  RNLSDVVSKLELRLSNIPTAVHRDVKLNTDASRRVVPEVVSLGGGKQDGLNDGGDGDLDT 120

Query: 121 EIQDGGRARAVSVTKYSPFWSERFHFLSAVKLDSDATCINVLPLRDFEGHSKFVAVGDER 180
            IQDGGR RAVSVTKYSPFWSERFHF+SAVKL+SDAT INVLPLRDFEGHSK+VAVGDE 
Sbjct: 121 RIQDGGRGRAVSVTKYSPFWSERFHFMSAVKLESDATSINVLPLRDFEGHSKYVAVGDET 180

Query: 181 GRVYVFTRNGDVAFELRTMSESPITAMLSYMSIYKNETLLVTGHKNGAILMHRIWEGSNG 240
           GR+YVF RNGDVA ELRTMS SPITAMLSYMSIYKNETLLVTGH++GAI MHRIWEGSNG
Sbjct: 181 GRIYVFVRNGDVAVELRTMSGSPITAMLSYMSIYKNETLLVTGHESGAISMHRIWEGSNG 240

Query: 241 EELNSLFMEHVVEFVATDGREDESPISTLEVHHVGRTRYILSSDARGKIKVFRENGTVYG 300
           EE NS+FMEHV+EFV TDGRE++SPIS LEVHHVGRTRYILSSD +GKIKVFRE+GTVYG
Sbjct: 241 EESNSIFMEHVLEFVVTDGREEKSPISILEVHHVGRTRYILSSDVKGKIKVFREDGTVYG 300

Query: 301 SVMATSRPIAFLKQRLLFLTESGAGSLDLRSMKLRESECEGLNYSLARNYVFDATERSKA 360
           SVM TSRPIAFLKQRLLFLTE+GAGSLDLRSMKLRESECEGLNYSLARNYVFDATERSKA
Sbjct: 301 SVMPTSRPIAFLKQRLLFLTETGAGSLDLRSMKLRESECEGLNYSLARNYVFDATERSKA 360

Query: 361 YGLTSDGDLIHVLLLGDIMNFKCRVRSKRKFEFDEPLGFQAIKGYLLVISNEKVHIFNVS 420
           YG TSDGDLIHV+LLGDIMNFKCRVRSK+KFE DEPL FQA+KGYLLVI +EKVH+FNVS
Sbjct: 361 YGFTSDGDLIHVMLLGDIMNFKCRVRSKKKFELDEPLLFQAVKGYLLVIGSEKVHVFNVS 420

Query: 421 SQHYVRVGAPRHLFSAGLDEIRSSFLNNQNLDLDSEGKFTPLISSDREKLVVIGLGGGYV 480
           SQHYVRVGAPR LFSA LDEIRSSFLNNQN DL+S+GKF PLISSDREKLVV+GLGGGY+
Sbjct: 421 SQHYVRVGAPRLLFSARLDEIRSSFLNNQNSDLESQGKFIPLISSDREKLVVLGLGGGYI 480

Query: 481 GMYRSNLPIFKGEFNTMLWTSPVLFFILFLFGAWQFFAKKKEALTSWGPDDPFTATSPTT 540
           GMYRSNLPI+KGEFNTMLWTSPVLFFILFLFGAWQFFAKKKEALTSWGPDDPFTATSPTT
Sbjct: 481 GMYRSNLPIYKGEFNTMLWTSPVLFFILFLFGAWQFFAKKKEALTSWGPDDPFTATSPTT 540

Query: 541 GAPLVTGSSE--------------RASFIDTASRSTDVMDLRGGSGLRGPPRRYGSPTGY 600
           GAPL TGS+E              R SFIDT SRSTD+MDLRGGSGLRGPPRRYGSPTGY
Sbjct: 541 GAPLGTGSTERTSFIDTPSRSTDMRTSFIDTPSRSTDMMDLRGGSGLRGPPRRYGSPTGY 600

Query: 601 PGGATSSFRPATTSDHNSRPAAVDPNYRAASELKFRGSPLEPPGFPKRREPLFANNQVVN 654
           PGG TSSFRPATTSDH+SR AAVDPNYRAASELKFRGSPLEPPGFPKRREPLFANNQVVN
Sbjct: 601 PGGVTSSFRPATTSDHSSRQAAVDPNYRAASELKFRGSPLEPPGFPKRREPLFANNQVVN 660

BLAST of Lag0004365 vs. NCBI nr
Match: XP_022962086.1 (uncharacterized membrane protein At1g75140-like [Cucurbita moschata])

HSP 1 Score: 1145.6 bits (2962), Expect = 0.0e+00
Identity = 576/667 (86.36%), Postives = 611/667 (91.60%), Query Frame = 0

Query: 1   MANPLKGKLLLIFFLFVFAIPHVSRVFVCSSSSSEQNEPEPEVPFEIRQYDLLKNLEELV 60
           MANP KGKLL I+FLFVF IP+VSR FVC SSS E N+ E EVP+E RQYD+LKNLEELV
Sbjct: 1   MANPWKGKLLFIYFLFVFGIPYVSRDFVCCSSSLELNQSETEVPYETRQYDVLKNLEELV 60

Query: 61  RNLSDVVSKLELRLSDIPTAVHRDKKLNTDALRRVVPEVVGSGGGKEDGISDGGDGGLDN 120
           RNLSDVVSKLELRLS+IPTAVHRD KLNTDA RRVVPEVV  GGGKEDG++DGG G LD 
Sbjct: 61  RNLSDVVSKLELRLSNIPTAVHRDVKLNTDASRRVVPEVVSLGGGKEDGLNDGGHGALDT 120

Query: 121 EIQDGGRARAVSVTKYSPFWSERFHFLSAVKLDSDATCINVLPLRDFEGHSKFVAVGDER 180
            +QDGGR RAVSVTKYSPFWSERFHFLSAVKL+SDAT INVLPLRDFEGHSK+VAVGDE 
Sbjct: 121 RVQDGGRGRAVSVTKYSPFWSERFHFLSAVKLESDATSINVLPLRDFEGHSKYVAVGDET 180

Query: 181 GRVYVFTRNGDVAFELRTMSESPITAMLSYMSIYKNETLLVTGHKNGAILMHRIWEGSNG 240
           GR+YVF RNGDVA ELRT+S SPITAMLSYMSIYKNETLLVTGH++GAI MHRIWEGSNG
Sbjct: 181 GRIYVFVRNGDVAVELRTVSGSPITAMLSYMSIYKNETLLVTGHESGAISMHRIWEGSNG 240

Query: 241 EELNSLFMEHVVEFVATDGREDESPISTLEVHHVGRTRYILSSDARGKIKVFRENGTVYG 300
           EE NS+FMEHV+EFV TDGRE++SPIS LEVHHVGRTRYILSSD +GKIKVFRE+GTVYG
Sbjct: 241 EESNSIFMEHVLEFVVTDGREEKSPISILEVHHVGRTRYILSSDVKGKIKVFREDGTVYG 300

Query: 301 SVMATSRPIAFLKQRLLFLTESGAGSLDLRSMKLRESECEGLNYSLARNYVFDATERSKA 360
           SVM TSRPIAFLKQRLLFLTE+GAGSLDLRSMKLRESECEGLNYSLARNYVFDATERSKA
Sbjct: 301 SVMPTSRPIAFLKQRLLFLTETGAGSLDLRSMKLRESECEGLNYSLARNYVFDATERSKA 360

Query: 361 YGLTSDGDLIHVLLLGDIMNFKCRVRSKRKFEFDEPLGFQAIKGYLLVISNEKVHIFNVS 420
           YG TSDGDLIHV+LLGDIMNFKCRVRSK+KFE DEPL FQA+KGYLLVI +EKVH+FNVS
Sbjct: 361 YGFTSDGDLIHVMLLGDIMNFKCRVRSKKKFELDEPLFFQAVKGYLLVIGSEKVHVFNVS 420

Query: 421 SQHYVRVGAPRHLFSAGLDEIRSSFLNNQNLDLDSEGKFTPLISSDREKLVVIGLGGGYV 480
           SQHYVRVGAPR LFSA LDEIRSSFLNNQNLDL+S+GKF PLISSDREKLVV+GLGGGY+
Sbjct: 421 SQHYVRVGAPRLLFSARLDEIRSSFLNNQNLDLESQGKFIPLISSDREKLVVLGLGGGYI 480

Query: 481 GMYRSNLPIFKGEFNTMLWTSPVLFFILFLFGAWQFFAKKKEALTSWGPDDPFTATSPTT 540
           GMYRSNLPI+KGEFNTMLWTSPVLFFILFLFGAWQFFAKKKEALTSWGPDDPFTATSPTT
Sbjct: 481 GMYRSNLPIYKGEFNTMLWTSPVLFFILFLFGAWQFFAKKKEALTSWGPDDPFTATSPTT 540

Query: 541 GAPLVTGSSE--------------RASFIDTASRSTDVMDLRGGSGLRGPPRRYGSPTGY 600
           GAPL TGS+E              R SFIDT SRSTD+MDLRGGSGLRGPPRRYGSPTGY
Sbjct: 541 GAPLGTGSTERTSFIDTPSRSTDMRTSFIDTPSRSTDMMDLRGGSGLRGPPRRYGSPTGY 600

Query: 601 PGGATSSFRPATTSDHNSRPAAVDPNYRAASELKFRGSPLEPPGFPKRREPLFANNQVVN 654
           PGG TSSFRPATTSDH+SR AAVDPNYRAASELKFRGSPLEPPGFPKRREPLFANNQVVN
Sbjct: 601 PGGVTSSFRPATTSDHSSRQAAVDPNYRAASELKFRGSPLEPPGFPKRREPLFANNQVVN 660

BLAST of Lag0004365 vs. ExPASy Swiss-Prot
Match: Q9FRK5 (Uncharacterized membrane protein At1g75140 OS=Arabidopsis thaliana OX=3702 GN=At1g75140 PE=1 SV=3)

HSP 1 Score: 648.7 bits (1672), Expect = 7.2e-185
Identity = 364/668 (54.49%), Postives = 458/668 (68.56%), Query Frame = 0

Query: 1   MANPLKGKLLLIFF----LFVFAIPHVSRVFVCSSS--SSEQNEPEPEVPFEI---RQYD 60
           MA+   GK    FF    + +F  P  S V    S     E ++  P V       RQ  
Sbjct: 1   MADSQNGKSAFFFFFVSLILLFLSPSYSDVTASESDPIPYENSDASPGVVTSSESDRQGV 60

Query: 61  LLKNLEELVRNLSDVVSKLELRLSDIPTAVHRDKKLNTDALRRVVPEVVGSGGGKEDGIS 120
            L  LEELVRNL+++V++L+ +LS+ P  V   K++  D                     
Sbjct: 61  SLHRLEELVRNLTELVARLDAKLSETPFKV--KKEITRD--------------------- 120

Query: 121 DGGDGGLDNEIQDGGRARAVSVTKYSPFWSERFHFLSAVKLDSDATCINVLPLRDFEGHS 180
                    EI++  +A+A SVTKYSPFWSERF F SAVKLDS+ATCINVLP RD EG S
Sbjct: 121 ---------EIEE--KAKAFSVTKYSPFWSERFEFTSAVKLDSEATCINVLPFRDHEGLS 180

Query: 181 KFVAVGDERGRVYVFTRNGDVAFELRTMSESPITAMLSYMSIYKNETLLVTGHKNGAILM 240
           K+ AVGD  GRV+VF RNGDV  E  T  +SPITAM+SYMS+YKNE+ +VTGH++G IL+
Sbjct: 181 KYFAVGDSSGRVFVFLRNGDVLVEFFTTCDSPITAMVSYMSVYKNESFVVTGHQSGVILL 240

Query: 241 HRIWEGSNGEELNSLFMEHVVEFVATDGREDESPISTLEVHHVGRTRYILSSDARGKIKV 300
           HR+ EGS GE+LNS  ME+V +F   DG ED   ++ LEVHHVGR RYIL++D  GK+ V
Sbjct: 241 HRLREGSIGEDLNSAVMENVGKF---DGTEDGLQVTLLEVHHVGRVRYILATDLSGKLTV 300

Query: 301 FRENGTVYGSVMATSRPIAFLKQRLLFLTESGAGSLDLRSMKLRESECEGLNYSLARNYV 360
           F EN TVYGSV  TSRP+ FLKQRLLFLTE+GAGSLDLRSMK+RESECEGLN+SLAR+YV
Sbjct: 301 FTENRTVYGSVSPTSRPLVFLKQRLLFLTETGAGSLDLRSMKIRESECEGLNHSLARSYV 360

Query: 361 FDATERSKAYGLTSDGDLIHVLLLGDIMNFKCRVRSKRKFEFDEPLGFQAIKGYLLVISN 420
           FDA+ER+KAYG TS+G++IHVLLLGDIMNFKCRVRSK+K + +EP+  QAIKGYLL+++ 
Sbjct: 361 FDASERAKAYGFTSEGEIIHVLLLGDIMNFKCRVRSKKKVQMEEPVALQAIKGYLLIVNQ 420

Query: 421 EKVHIFNVSSQHYVRVGAPRHLFSAGLDEIRSSFLNN----QNLDLDSEGKFTPLISSDR 480
           EKV ++NVS+QHYVR   PR LF A L++IRS+FL++    +  D     K TPLI+SDR
Sbjct: 421 EKVFVYNVSTQHYVRTTGPRLLFPAALEDIRSTFLSHRESTKTTDHQKLEKVTPLIASDR 480

Query: 481 EKLVVIGLGGGYVGMYRSNLPIFKGEFNTMLWTSPVLFFILFLFGAWQFFAKKKEALTSW 540
           EKL+V+GLG GYV  Y+S LPI K EFNTMLW+SPV FFILFLFGAW FF+KKKE+LT+W
Sbjct: 481 EKLLVMGLGDGYVATYKSKLPISKAEFNTMLWSSPVFFFILFLFGAWHFFSKKKESLTAW 540

Query: 541 GPDDPFTATSPTTGAPLVTGSSERASFIDTASRSTDVMDLRGGSGLRGPPRRYGSPTGY- 600
           GPDDPF++T  T  +   T +   ++F ++  R+ D MDLR         RRY SP+ Y 
Sbjct: 541 GPDDPFSST--TMSSSSTTTAQNSSAFSESTRRNDDHMDLR---------RRYVSPSRYP 600

Query: 601 PGGATSSFRPATTSDHNSRPAAVDPNYR-AASELKFR-GSPLEPPGFPKRREPLFANNQV 653
           PG AT ++R   ++D +SR      NYR  A E+K+R GS L+  GF KRRE LF NN+ 
Sbjct: 601 PGAATGAYRSVGSNDPSSRAPVETTNYRTTAQEMKYRGGSGLDSGGFGKRRESLFGNNKA 617

BLAST of Lag0004365 vs. ExPASy TrEMBL
Match: A0A6J1CSU7 (uncharacterized membrane protein At1g75140-like OS=Momordica charantia OX=3673 GN=LOC111013986 PE=4 SV=1)

HSP 1 Score: 1159.1 bits (2997), Expect = 0.0e+00
Identity = 589/657 (89.65%), Postives = 610/657 (92.85%), Query Frame = 0

Query: 1   MANPLKGKLLLIFFLFVFAIPHVSRVFVCSSSSSEQNEP----EPEVPFEIRQYDLLKNL 60
           MANP KGKLLLI+FLFVF +PHVSRVFVCSSSS EQN+P    E EVPFEIRQ DLLKNL
Sbjct: 1   MANPRKGKLLLIYFLFVFGVPHVSRVFVCSSSSFEQNQPESESESEVPFEIRQRDLLKNL 60

Query: 61  EELVRNLSDVVSKLELRLSDIPTAVHRDKKLNTDALRRVVPEVVGSGGGKEDGISDGGDG 120
           EELVRNLSDVVSKLE+RLSDIPT V RDKK NTDA RRVVPEVV  GG KEDG++DGGDG
Sbjct: 61  EELVRNLSDVVSKLEMRLSDIPTEVIRDKKPNTDASRRVVPEVVSLGGRKEDGLNDGGDG 120

Query: 121 GLDNEIQDGGRARAVSVTKYSPFWSERFHFLSAVKLDSDATCINVLPLRDFEGHSKFVAV 180
           GL+++I+DG R RAVSVTKYSPFWSERFHFLSAVKLDSDATCINVLP RDFEGHSK+V V
Sbjct: 121 GLESKIRDGDRTRAVSVTKYSPFWSERFHFLSAVKLDSDATCINVLPFRDFEGHSKYVVV 180

Query: 181 GDERGRVYVFTRNGDVAFELRTMSESPITAMLSYMSIYKNETLLVTGHKNGAILMHRIWE 240
           GDE+GRVYVFTRNGDVA ELRT SESPI  MLSYMSIYKNETLLVTGHKNGAILMHRIWE
Sbjct: 181 GDEKGRVYVFTRNGDVAVELRTKSESPIMTMLSYMSIYKNETLLVTGHKNGAILMHRIWE 240

Query: 241 GSNGEELNSLFMEHVVEFVATDGREDESPISTLEVHHVGRTRYILSSDARGKIKVFRENG 300
            SNGEE +SLFMEHVVEFVATDGREDESPIS LEVHHVGRTRYILSSD RGKIKVFRENG
Sbjct: 241 RSNGEESHSLFMEHVVEFVATDGREDESPISILEVHHVGRTRYILSSDVRGKIKVFRENG 300

Query: 301 TVYGSVMATSRPIAFLKQRLLFLTESGAGSLDLRSMKLRESECEGLNYSLARNYVFDATE 360
           TVYGSVM  S+PIAFLKQRLLFLTESGAGSLDLRSMKLRESECEGLNYSLARNYVFDATE
Sbjct: 301 TVYGSVMPESKPIAFLKQRLLFLTESGAGSLDLRSMKLRESECEGLNYSLARNYVFDATE 360

Query: 361 RSKAYGLTSDGDLIHVLLLGDIMNFKCRVRSKRKFEFDEPLGFQAIKGYLLVISNEKVHI 420
           RSKAYG TS GDLIHVLLLGDIMNFKCRVRSKRKFE DEPL FQAIKGYLLVISNEKVHI
Sbjct: 361 RSKAYGFTSTGDLIHVLLLGDIMNFKCRVRSKRKFELDEPLVFQAIKGYLLVISNEKVHI 420

Query: 421 FNVSSQHYVRVGAPRHLFSAGLDEIRSSFLNNQNLDLDSEGKFTPLISSDREKLVVIGLG 480
           FNVSSQHY RVGAPR LFSA +DEIRSSFLN QNLDL+SEGKF PLISSDREKLVV+GLG
Sbjct: 421 FNVSSQHYARVGAPRPLFSAAVDEIRSSFLNYQNLDLESEGKFVPLISSDREKLVVLGLG 480

Query: 481 GGYVGMYRSNLPIFKGEFNTMLWTSPVLFFILFLFGAWQFFAKKKEALTSWGPDDPFTAT 540
           GGYVGMYRSNLPIFKGEFNTMLWTSPVLFFILFLFGAW FFAKKKEALTSWGPDDPFTAT
Sbjct: 481 GGYVGMYRSNLPIFKGEFNTMLWTSPVLFFILFLFGAWHFFAKKKEALTSWGPDDPFTAT 540

Query: 541 SPTTGAPLVTGSSERASFIDTASRSTDVMDLRGGSGLRGPPRRYGSPTGYPGGATSSFRP 600
           SPTTGAPL TG+SER+SFIDT SRSTD+MDLRGGSGLRGPPRRYGSPTGYPGGAT+SFRP
Sbjct: 541 SPTTGAPLGTGTSERSSFIDTPSRSTDMMDLRGGSGLRGPPRRYGSPTGYPGGATNSFRP 600

Query: 601 ATTSDHNSRPAAVDPNYRAASELKFRGSPLEPPGFPKRREPLFANNQVVNQVVDESS 654
            TTSDHNSRPAAVDPNYRAASELKFRGSPLEPPGFPKRREPLFAN    NQVVDESS
Sbjct: 601 PTTSDHNSRPAAVDPNYRAASELKFRGSPLEPPGFPKRREPLFAN----NQVVDESS 653

BLAST of Lag0004365 vs. ExPASy TrEMBL
Match: A0A6J1KBU3 (uncharacterized membrane protein At1g75140-like OS=Cucurbita maxima OX=3661 GN=LOC111491879 PE=4 SV=1)

HSP 1 Score: 1148.7 bits (2970), Expect = 0.0e+00
Identity = 580/667 (86.96%), Postives = 611/667 (91.60%), Query Frame = 0

Query: 1   MANPLKGKLLLIFFLFVFAIPHVSRVFVCSSSSSEQNEPEPEVPFEIRQYDLLKNLEELV 60
           MANP KGKLL I+FLFVF IPHVSR FVC SSS E N+ E EVPFE RQYDLLKNLEELV
Sbjct: 1   MANPRKGKLLFIYFLFVFGIPHVSRDFVCCSSSFELNQSETEVPFETRQYDLLKNLEELV 60

Query: 61  RNLSDVVSKLELRLSDIPTAVHRDKKLNTDALRRVVPEVVGSGGGKEDGISDGGDGGLDN 120
           RNLSDVVSKLELRLS+IPTAVHRD KLNTDA RRVVPEVV  GGGKEDG++DGGDG LD 
Sbjct: 61  RNLSDVVSKLELRLSNIPTAVHRDVKLNTDASRRVVPEVVSLGGGKEDGLNDGGDGALDT 120

Query: 121 EIQDGGRARAVSVTKYSPFWSERFHFLSAVKLDSDATCINVLPLRDFEGHSKFVAVGDER 180
           +IQDGGR RAVSVTKYSPFWSERFHFLSAVKL+SDAT INVLPLRDFEGHSK+VAVGDE 
Sbjct: 121 KIQDGGRGRAVSVTKYSPFWSERFHFLSAVKLESDATSINVLPLRDFEGHSKYVAVGDET 180

Query: 181 GRVYVFTRNGDVAFELRTMSESPITAMLSYMSIYKNETLLVTGHKNGAILMHRIWEGSNG 240
           GR+YVF RNGDVA ELRTMS SPITA LSYMSIYKNETLLVTGH++GAI M RIWEGSNG
Sbjct: 181 GRIYVFVRNGDVAVELRTMSGSPITATLSYMSIYKNETLLVTGHESGAISMLRIWEGSNG 240

Query: 241 EELNSLFMEHVVEFVATDGREDESPISTLEVHHVGRTRYILSSDARGKIKVFRENGTVYG 300
           EE NS+FMEHV+EFV TDGRE++SPIS LEVHHVGRTRYILSSD +GKIKVFRE+GTVYG
Sbjct: 241 EESNSIFMEHVLEFVVTDGREEKSPISILEVHHVGRTRYILSSDVKGKIKVFREDGTVYG 300

Query: 301 SVMATSRPIAFLKQRLLFLTESGAGSLDLRSMKLRESECEGLNYSLARNYVFDATERSKA 360
           SVM TSRPIAFLKQRLLFLTE+GAGSLDLRSMKLRESECEGLNYSLARNYVFDATERSKA
Sbjct: 301 SVMPTSRPIAFLKQRLLFLTETGAGSLDLRSMKLRESECEGLNYSLARNYVFDATERSKA 360

Query: 361 YGLTSDGDLIHVLLLGDIMNFKCRVRSKRKFEFDEPLGFQAIKGYLLVISNEKVHIFNVS 420
           YG TSDGDLIHV+LLGDIMNFKCRVRSK+KFE DEPL FQA+KGYLLVI +EKVH+FNVS
Sbjct: 361 YGFTSDGDLIHVMLLGDIMNFKCRVRSKKKFELDEPLFFQAVKGYLLVIGSEKVHVFNVS 420

Query: 421 SQHYVRVGAPRHLFSAGLDEIRSSFLNNQNLDLDSEGKFTPLISSDREKLVVIGLGGGYV 480
           SQHYVRVGAPR LFSA LDEIRSSFLNNQNLDL+S+GKF PLISSDREKLVV+GLGGGY+
Sbjct: 421 SQHYVRVGAPRLLFSARLDEIRSSFLNNQNLDLESQGKFIPLISSDREKLVVLGLGGGYI 480

Query: 481 GMYRSNLPIFKGEFNTMLWTSPVLFFILFLFGAWQFFAKKKEALTSWGPDDPFTATSPTT 540
           GMYRSNLPI+KGEFNTMLWTSPVLFFILFLFGAWQFFAKKKEALTSWGPDDPFTATSPTT
Sbjct: 481 GMYRSNLPIYKGEFNTMLWTSPVLFFILFLFGAWQFFAKKKEALTSWGPDDPFTATSPTT 540

Query: 541 GAPLVTGSSE--------------RASFIDTASRSTDVMDLRGGSGLRGPPRRYGSPTGY 600
           GAPL TGS+E              R SFIDT SRSTD+MDLRGGSGLRGPPRRYGSPTGY
Sbjct: 541 GAPLGTGSTERTSFIDTPSRSTDMRTSFIDTPSRSTDMMDLRGGSGLRGPPRRYGSPTGY 600

Query: 601 PGGATSSFRPATTSDHNSRPAAVDPNYRAASELKFRGSPLEPPGFPKRREPLFANNQVVN 654
           PGG TSSFRPATTSDH+SR AAVDPNYRAASELKFRGSPLEPPGFPKRREPLFANNQVVN
Sbjct: 601 PGGVTSSFRPATTSDHSSRQAAVDPNYRAASELKFRGSPLEPPGFPKRREPLFANNQVVN 660

BLAST of Lag0004365 vs. ExPASy TrEMBL
Match: A0A6J1HC37 (uncharacterized membrane protein At1g75140-like OS=Cucurbita moschata OX=3662 GN=LOC111462650 PE=4 SV=1)

HSP 1 Score: 1145.6 bits (2962), Expect = 0.0e+00
Identity = 576/667 (86.36%), Postives = 611/667 (91.60%), Query Frame = 0

Query: 1   MANPLKGKLLLIFFLFVFAIPHVSRVFVCSSSSSEQNEPEPEVPFEIRQYDLLKNLEELV 60
           MANP KGKLL I+FLFVF IP+VSR FVC SSS E N+ E EVP+E RQYD+LKNLEELV
Sbjct: 1   MANPWKGKLLFIYFLFVFGIPYVSRDFVCCSSSLELNQSETEVPYETRQYDVLKNLEELV 60

Query: 61  RNLSDVVSKLELRLSDIPTAVHRDKKLNTDALRRVVPEVVGSGGGKEDGISDGGDGGLDN 120
           RNLSDVVSKLELRLS+IPTAVHRD KLNTDA RRVVPEVV  GGGKEDG++DGG G LD 
Sbjct: 61  RNLSDVVSKLELRLSNIPTAVHRDVKLNTDASRRVVPEVVSLGGGKEDGLNDGGHGALDT 120

Query: 121 EIQDGGRARAVSVTKYSPFWSERFHFLSAVKLDSDATCINVLPLRDFEGHSKFVAVGDER 180
            +QDGGR RAVSVTKYSPFWSERFHFLSAVKL+SDAT INVLPLRDFEGHSK+VAVGDE 
Sbjct: 121 RVQDGGRGRAVSVTKYSPFWSERFHFLSAVKLESDATSINVLPLRDFEGHSKYVAVGDET 180

Query: 181 GRVYVFTRNGDVAFELRTMSESPITAMLSYMSIYKNETLLVTGHKNGAILMHRIWEGSNG 240
           GR+YVF RNGDVA ELRT+S SPITAMLSYMSIYKNETLLVTGH++GAI MHRIWEGSNG
Sbjct: 181 GRIYVFVRNGDVAVELRTVSGSPITAMLSYMSIYKNETLLVTGHESGAISMHRIWEGSNG 240

Query: 241 EELNSLFMEHVVEFVATDGREDESPISTLEVHHVGRTRYILSSDARGKIKVFRENGTVYG 300
           EE NS+FMEHV+EFV TDGRE++SPIS LEVHHVGRTRYILSSD +GKIKVFRE+GTVYG
Sbjct: 241 EESNSIFMEHVLEFVVTDGREEKSPISILEVHHVGRTRYILSSDVKGKIKVFREDGTVYG 300

Query: 301 SVMATSRPIAFLKQRLLFLTESGAGSLDLRSMKLRESECEGLNYSLARNYVFDATERSKA 360
           SVM TSRPIAFLKQRLLFLTE+GAGSLDLRSMKLRESECEGLNYSLARNYVFDATERSKA
Sbjct: 301 SVMPTSRPIAFLKQRLLFLTETGAGSLDLRSMKLRESECEGLNYSLARNYVFDATERSKA 360

Query: 361 YGLTSDGDLIHVLLLGDIMNFKCRVRSKRKFEFDEPLGFQAIKGYLLVISNEKVHIFNVS 420
           YG TSDGDLIHV+LLGDIMNFKCRVRSK+KFE DEPL FQA+KGYLLVI +EKVH+FNVS
Sbjct: 361 YGFTSDGDLIHVMLLGDIMNFKCRVRSKKKFELDEPLFFQAVKGYLLVIGSEKVHVFNVS 420

Query: 421 SQHYVRVGAPRHLFSAGLDEIRSSFLNNQNLDLDSEGKFTPLISSDREKLVVIGLGGGYV 480
           SQHYVRVGAPR LFSA LDEIRSSFLNNQNLDL+S+GKF PLISSDREKLVV+GLGGGY+
Sbjct: 421 SQHYVRVGAPRLLFSARLDEIRSSFLNNQNLDLESQGKFIPLISSDREKLVVLGLGGGYI 480

Query: 481 GMYRSNLPIFKGEFNTMLWTSPVLFFILFLFGAWQFFAKKKEALTSWGPDDPFTATSPTT 540
           GMYRSNLPI+KGEFNTMLWTSPVLFFILFLFGAWQFFAKKKEALTSWGPDDPFTATSPTT
Sbjct: 481 GMYRSNLPIYKGEFNTMLWTSPVLFFILFLFGAWQFFAKKKEALTSWGPDDPFTATSPTT 540

Query: 541 GAPLVTGSSE--------------RASFIDTASRSTDVMDLRGGSGLRGPPRRYGSPTGY 600
           GAPL TGS+E              R SFIDT SRSTD+MDLRGGSGLRGPPRRYGSPTGY
Sbjct: 541 GAPLGTGSTERTSFIDTPSRSTDMRTSFIDTPSRSTDMMDLRGGSGLRGPPRRYGSPTGY 600

Query: 601 PGGATSSFRPATTSDHNSRPAAVDPNYRAASELKFRGSPLEPPGFPKRREPLFANNQVVN 654
           PGG TSSFRPATTSDH+SR AAVDPNYRAASELKFRGSPLEPPGFPKRREPLFANNQVVN
Sbjct: 601 PGGVTSSFRPATTSDHSSRQAAVDPNYRAASELKFRGSPLEPPGFPKRREPLFANNQVVN 660

BLAST of Lag0004365 vs. ExPASy TrEMBL
Match: A0A0A0LNK9 (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_2G361500 PE=4 SV=1)

HSP 1 Score: 1122.8 bits (2903), Expect = 0.0e+00
Identity = 569/654 (87.00%), Postives = 601/654 (91.90%), Query Frame = 0

Query: 1   MANPLKGKLLLIFFLFVFAIPHVSRVFVCSSSSSEQNEPEPEVPFEIRQYDLLKNLEELV 60
           MA   KG+   IFFLF+FA PHVSRVFVCSSSS EQN+ E EVPFEI+QYDLLKNLEELV
Sbjct: 1   MAIERKGRFFFIFFLFIFAFPHVSRVFVCSSSSLEQNQSEIEVPFEIKQYDLLKNLEELV 60

Query: 61  RNLSDVVSKLELRLSDIPTAVHRDKKLNTDALRRVVPEV-VGSGGGKEDGISDGGDGGLD 120
           RNLSDVVSKLELRLSDIPTAV+R+ KLNTDA RRVVPEV      GKE+G++DGGDGGLD
Sbjct: 61  RNLSDVVSKLELRLSDIPTAVNRE-KLNTDAFRRVVPEVGKEKRKGKEEGLNDGGDGGLD 120

Query: 121 NEIQDGGRARAVSVTKYSPFWSERFHFLSAVKLDSDATCINVLPLRDFEGHSKFVAVGDE 180
           ++IQDGGR RAVSVTKYS FWSERFHFLSAVKL++DATCINVLPLRDFEGHSK+VAVGDE
Sbjct: 121 HKIQDGGRTRAVSVTKYSSFWSERFHFLSAVKLEADATCINVLPLRDFEGHSKYVAVGDE 180

Query: 181 RGRVYVFTRNGDVAFELRTMSESPITAMLSYMSIYKNETLLVTGHKNGAILMHRIWEGSN 240
           RGR+YVF  NGDVA EL T+  SP+TAMLSYMSIYKNETLLVTGHKNGAILMHRIWEGSN
Sbjct: 181 RGRIYVFVINGDVAIELPTVPGSPVTAMLSYMSIYKNETLLVTGHKNGAILMHRIWEGSN 240

Query: 241 GEELNSLFMEHVVEFVATDGREDESPISTLEVHHVGRTRYILSSDARGKIKVFRENGTVY 300
           GE+LN +FMEHVVEFVAT+ REDES IS LE+HHVGRTRYILSSD  GKIKVFRE+GTVY
Sbjct: 241 GEDLNLIFMEHVVEFVATNSREDESQISLLELHHVGRTRYILSSDFGGKIKVFREDGTVY 300

Query: 301 GSVMATSRPIAFLKQRLLFLTESGAGSLDLRSMKLRESECEGLNYSLARNYVFDATERSK 360
           GSVM TSRPIAFLKQRLLFLTESGAGSLDLRSMKLRESECEGLN+SLARNYVFDA ERSK
Sbjct: 301 GSVMPTSRPIAFLKQRLLFLTESGAGSLDLRSMKLRESECEGLNHSLARNYVFDAMERSK 360

Query: 361 AYGLTSDGDLIHVLLLGDIMNFKCRVRSKRKFEFDEPLGFQAIKGYLLVISNEKVHIFNV 420
           AYG+TSDGDLIHVLLLGDIMNFKCRVRSKRKFE DEPL FQ IKGYLLV SNEKVH+FNV
Sbjct: 361 AYGVTSDGDLIHVLLLGDIMNFKCRVRSKRKFELDEPLAFQTIKGYLLVTSNEKVHVFNV 420

Query: 421 SSQHYVRVGAPRHLFSAGLDEIRSSFLNNQNLDLDSEGKFTPLISSDREKLVVIGLGGGY 480
           SSQHYVRVGAPR LFSAGLDEI+SSFLN QN DL SE  F PLISSD EKLVV+GLGGGY
Sbjct: 421 SSQHYVRVGAPRLLFSAGLDEIKSSFLNYQNSDLVSEANFIPLISSDHEKLVVLGLGGGY 480

Query: 481 VGMYRSNLPIFKGEFNTMLWTSPVLFFILFLFGAWQFFAKKKEALTSWGPDDPFTATSPT 540
           VGMYRSNLPIFKGEFNTM+WTSPVLFFILFLFGAW FFAKKKEALTSWGPDDPFTATSPT
Sbjct: 481 VGMYRSNLPIFKGEFNTMVWTSPVLFFILFLFGAWHFFAKKKEALTSWGPDDPFTATSPT 540

Query: 541 TGAPLVTGSSERASFIDTASRSTDVMDLRGGSGLRGPPRRYGSPTGYPGGATSSFRPATT 600
           TGAP+ TGSSERASFIDT SRSTDVMDLR   GLRGPPRRYGSPTGYP GATSSFRPATT
Sbjct: 541 TGAPMGTGSSERASFIDTPSRSTDVMDLRSAGGLRGPPRRYGSPTGYPAGATSSFRPATT 600

Query: 601 SDHNSRPAAVDPNYRAASELKFRGSPLEPPGFPKRREPLFANNQVVNQVVDESS 654
           +DH+SRPAAVDPNYRAASELKFRGSPLEPPGFPKRREPLFANNQVVNQVVD+SS
Sbjct: 601 NDHSSRPAAVDPNYRAASELKFRGSPLEPPGFPKRREPLFANNQVVNQVVDDSS 653

BLAST of Lag0004365 vs. ExPASy TrEMBL
Match: A0A1S3BAX2 (uncharacterized membrane protein At1g75140-like OS=Cucumis melo OX=3656 GN=LOC103487958 PE=4 SV=1)

HSP 1 Score: 1105.1 bits (2857), Expect = 0.0e+00
Identity = 569/657 (86.61%), Postives = 597/657 (90.87%), Query Frame = 0

Query: 1   MANPLKGKLLLIFFLFVFAIPHVSRVFVCSSSSSEQNEPEPEVPFEIRQYDLLKNLEELV 60
           MA   KG+   IFFLF+FA PHVSRVFVCSS+S EQN+ E EVPFEI+QYDLLKNLEELV
Sbjct: 1   MAIERKGRFFFIFFLFIFAFPHVSRVFVCSSNSFEQNQSEIEVPFEIKQYDLLKNLEELV 60

Query: 61  RNLSDVVSKLELRLSDIPTAVHRDKKLNTDALRRVVPEV-VGSGGGKEDGISDGGDGGLD 120
           RNLSDVVSKLELRLSDIPTAV+R+ KLN DA RRVVPEV    G GKED   D  D GLD
Sbjct: 61  RNLSDVVSKLELRLSDIPTAVNRE-KLNIDASRRVVPEVGKEKGKGKED---DPNDVGLD 120

Query: 121 NEIQDGGRARAVSVTKYSPFWSERFHFLSAVKLDSDATCINVLPLRDFEGHSKFVAVGDE 180
           ++IQDGGR RAVSVTKYS FWSERFHFLSAVKL+SDATC NVLPLRDFEG+SK+VAVGDE
Sbjct: 121 HKIQDGGRTRAVSVTKYSSFWSERFHFLSAVKLESDATCTNVLPLRDFEGNSKYVAVGDE 180

Query: 181 RGRVYVFTRNGDVAFELRTMSESPITAMLSYMSIYKNETLLVTGHKNGAILMHRIWEGSN 240
           RGR+YVF RNGDVA EL T+  SPITAMLSYMSIYKNETLLVTGHKNG ILMHRIWEGSN
Sbjct: 181 RGRIYVFVRNGDVAIELPTVPGSPITAMLSYMSIYKNETLLVTGHKNGGILMHRIWEGSN 240

Query: 241 GEELNSLFMEHVVEFVATDGREDE--SPISTLEVHHVGRTRYILSSDARGKIKVFRENGT 300
           GE+LN +FME VVEFVATD REDE  S IS LE+HHVGRTRYILSSD RGKIKVF+E+GT
Sbjct: 241 GEDLNLIFMERVVEFVATDSREDESQSQISLLELHHVGRTRYILSSDLRGKIKVFKEDGT 300

Query: 301 VYGSVMATSRPIAFLKQRLLFLTESGAGSLDLRSMKLRESECEGLNYSLARNYVFDATER 360
           VYGSVM TSRPIAFLKQRLLFLTESGAGSLDLRSMKLRESECEGLN+SL RNYVFDATER
Sbjct: 301 VYGSVMPTSRPIAFLKQRLLFLTESGAGSLDLRSMKLRESECEGLNHSLVRNYVFDATER 360

Query: 361 SKAYGLTSDGDLIHVLLLGDIMNFKCRVRSKRKFEFDEPLGFQAIKGYLLVISNEKVHIF 420
           SKAYG TSDGDLIHVLLLGDIMNFKCRVRSKRKFE DEPL FQ IKGYLLV SNEKVH+F
Sbjct: 361 SKAYGFTSDGDLIHVLLLGDIMNFKCRVRSKRKFELDEPLAFQTIKGYLLVTSNEKVHVF 420

Query: 421 NVSSQHYVRVGAPRHLFSAGLDEIRSSFLNNQNLDLDSEGKF-TPLISSDREKLVVIGLG 480
           NVSS HYVRVGAPR LFSAGLDEI+SSFLN QNLDL+SE  F  PLISSDREKLVV+GLG
Sbjct: 421 NVSSHHYVRVGAPRLLFSAGLDEIKSSFLNYQNLDLESEANFYIPLISSDREKLVVLGLG 480

Query: 481 GGYVGMYRSNLPIFKGEFNTMLWTSPVLFFILFLFGAWQFFAKKKEALTSWGPDDPFTAT 540
           GGYVGMYRSNLPIFKGEFNTMLWTSPVLFFILFLFGAW FFAKKKEALTSWGPDDPFTAT
Sbjct: 481 GGYVGMYRSNLPIFKGEFNTMLWTSPVLFFILFLFGAWHFFAKKKEALTSWGPDDPFTAT 540

Query: 541 SPTTGAPLVTGSSERASFIDTASRSTDVMDLRGGSGLRGPPRRYGSPTGYPGGATSSFRP 600
           SPTTGAP+ TGSSERASFIDT SRSTDVMDLRGG GLRGP RRYGSPTGYPGGATSSFRP
Sbjct: 541 SPTTGAPMGTGSSERASFIDTPSRSTDVMDLRGGGGLRGPARRYGSPTGYPGGATSSFRP 600

Query: 601 ATTSDHNSRPAAVDPNYRAASELKFRGSPLEPPGFPKRREPLFANNQVVNQVVDESS 654
           ATTSDH+SRPAAVDPNYRAASELKFRGSPLEPPGFPKRREPLFANNQVVNQVVD+S+
Sbjct: 601 ATTSDHSSRPAAVDPNYRAASELKFRGSPLEPPGFPKRREPLFANNQVVNQVVDDSN 653

BLAST of Lag0004365 vs. TAIR 10
Match: AT1G75140.1 (unknown protein; LOCATED IN: endoplasmic reticulum; EXPRESSED IN: 24 plant structures; EXPRESSED DURING: 13 growth stages; BEST Arabidopsis thaliana protein match is: unknown protein (TAIR:AT1G19370.1); Has 51 Blast hits to 49 proteins in 18 species: Archae - 0; Bacteria - 0; Metazoa - 0; Fungi - 0; Plants - 46; Viruses - 0; Other Eukaryotes - 5 (source: NCBI BLink). )

HSP 1 Score: 648.7 bits (1672), Expect = 5.1e-186
Identity = 364/668 (54.49%), Postives = 458/668 (68.56%), Query Frame = 0

Query: 1   MANPLKGKLLLIFF----LFVFAIPHVSRVFVCSSS--SSEQNEPEPEVPFEI---RQYD 60
           MA+   GK    FF    + +F  P  S V    S     E ++  P V       RQ  
Sbjct: 1   MADSQNGKSAFFFFFVSLILLFLSPSYSDVTASESDPIPYENSDASPGVVTSSESDRQGV 60

Query: 61  LLKNLEELVRNLSDVVSKLELRLSDIPTAVHRDKKLNTDALRRVVPEVVGSGGGKEDGIS 120
            L  LEELVRNL+++V++L+ +LS+ P  V   K++  D                     
Sbjct: 61  SLHRLEELVRNLTELVARLDAKLSETPFKV--KKEITRD--------------------- 120

Query: 121 DGGDGGLDNEIQDGGRARAVSVTKYSPFWSERFHFLSAVKLDSDATCINVLPLRDFEGHS 180
                    EI++  +A+A SVTKYSPFWSERF F SAVKLDS+ATCINVLP RD EG S
Sbjct: 121 ---------EIEE--KAKAFSVTKYSPFWSERFEFTSAVKLDSEATCINVLPFRDHEGLS 180

Query: 181 KFVAVGDERGRVYVFTRNGDVAFELRTMSESPITAMLSYMSIYKNETLLVTGHKNGAILM 240
           K+ AVGD  GRV+VF RNGDV  E  T  +SPITAM+SYMS+YKNE+ +VTGH++G IL+
Sbjct: 181 KYFAVGDSSGRVFVFLRNGDVLVEFFTTCDSPITAMVSYMSVYKNESFVVTGHQSGVILL 240

Query: 241 HRIWEGSNGEELNSLFMEHVVEFVATDGREDESPISTLEVHHVGRTRYILSSDARGKIKV 300
           HR+ EGS GE+LNS  ME+V +F   DG ED   ++ LEVHHVGR RYIL++D  GK+ V
Sbjct: 241 HRLREGSIGEDLNSAVMENVGKF---DGTEDGLQVTLLEVHHVGRVRYILATDLSGKLTV 300

Query: 301 FRENGTVYGSVMATSRPIAFLKQRLLFLTESGAGSLDLRSMKLRESECEGLNYSLARNYV 360
           F EN TVYGSV  TSRP+ FLKQRLLFLTE+GAGSLDLRSMK+RESECEGLN+SLAR+YV
Sbjct: 301 FTENRTVYGSVSPTSRPLVFLKQRLLFLTETGAGSLDLRSMKIRESECEGLNHSLARSYV 360

Query: 361 FDATERSKAYGLTSDGDLIHVLLLGDIMNFKCRVRSKRKFEFDEPLGFQAIKGYLLVISN 420
           FDA+ER+KAYG TS+G++IHVLLLGDIMNFKCRVRSK+K + +EP+  QAIKGYLL+++ 
Sbjct: 361 FDASERAKAYGFTSEGEIIHVLLLGDIMNFKCRVRSKKKVQMEEPVALQAIKGYLLIVNQ 420

Query: 421 EKVHIFNVSSQHYVRVGAPRHLFSAGLDEIRSSFLNN----QNLDLDSEGKFTPLISSDR 480
           EKV ++NVS+QHYVR   PR LF A L++IRS+FL++    +  D     K TPLI+SDR
Sbjct: 421 EKVFVYNVSTQHYVRTTGPRLLFPAALEDIRSTFLSHRESTKTTDHQKLEKVTPLIASDR 480

Query: 481 EKLVVIGLGGGYVGMYRSNLPIFKGEFNTMLWTSPVLFFILFLFGAWQFFAKKKEALTSW 540
           EKL+V+GLG GYV  Y+S LPI K EFNTMLW+SPV FFILFLFGAW FF+KKKE+LT+W
Sbjct: 481 EKLLVMGLGDGYVATYKSKLPISKAEFNTMLWSSPVFFFILFLFGAWHFFSKKKESLTAW 540

Query: 541 GPDDPFTATSPTTGAPLVTGSSERASFIDTASRSTDVMDLRGGSGLRGPPRRYGSPTGY- 600
           GPDDPF++T  T  +   T +   ++F ++  R+ D MDLR         RRY SP+ Y 
Sbjct: 541 GPDDPFSST--TMSSSSTTTAQNSSAFSESTRRNDDHMDLR---------RRYVSPSRYP 600

Query: 601 PGGATSSFRPATTSDHNSRPAAVDPNYR-AASELKFR-GSPLEPPGFPKRREPLFANNQV 653
           PG AT ++R   ++D +SR      NYR  A E+K+R GS L+  GF KRRE LF NN+ 
Sbjct: 601 PGAATGAYRSVGSNDPSSRAPVETTNYRTTAQEMKYRGGSGLDSGGFGKRRESLFGNNKA 617

BLAST of Lag0004365 vs. TAIR 10
Match: AT1G19370.1 (unknown protein; LOCATED IN: endoplasmic reticulum; EXPRESSED IN: 22 plant structures; EXPRESSED DURING: 13 growth stages; BEST Arabidopsis thaliana protein match is: unknown protein (TAIR:AT1G75140.1); Has 45 Blast hits to 43 proteins in 15 species: Archae - 0; Bacteria - 0; Metazoa - 0; Fungi - 0; Plants - 44; Viruses - 0; Other Eukaryotes - 1 (source: NCBI BLink). )

HSP 1 Score: 637.1 bits (1642), Expect = 1.5e-182
Identity = 354/658 (53.80%), Postives = 445/658 (67.63%), Query Frame = 0

Query: 1   MANPLKGKLLLIFF--LFVFAIPHVSRVFVCSSSSSEQNEPEPEVPFE--IRQYDLLKNL 60
           MA+PL GK   I F  LF F +  +S ++   S   E    +  V      R+  LL NL
Sbjct: 1   MADPLNGKSFFICFSLLFSFTLLFISPLYATESPVIEDVSTDVAVSVSETNREAVLLHNL 60

Query: 61  EELVRNLSDVVSKLELRLSDIPTAVHRDKKLNTDALRRVVPEVVGSGGGKEDGISDGGDG 120
           EELV+NL+++V+ L+ +LS  P     +  ++ D                          
Sbjct: 61  EELVKNLTELVANLDAKLSATPLKEKNEISVDDDI------------------------- 120

Query: 121 GLDNEIQDGGRARAVSVTKYSPFWSERFHFLSAVKLDSDATCINVLPLRDFEGHSKFVAV 180
               E ++ GRA+A SVTKYSPFWSERF F SAVKL+SDATCINVLP RDFEG SK+ A+
Sbjct: 121 ---GEEKERGRAKAFSVTKYSPFWSERFQFTSAVKLNSDATCINVLPFRDFEGSSKYFAI 180

Query: 181 GDERGRVYVFTRNGDVAFELRTMSESPITAMLSYMSIYKNETLLVTGHKNGAILMHRIWE 240
           GD +GRVYVF RNGDV  E  T  +SP+TAM+SY S++KN + +VTGH+NGA+L+HRI E
Sbjct: 181 GDSKGRVYVFLRNGDVLIEFFTTVDSPVTAMVSYSSVFKNSSFVVTGHQNGAVLLHRIHE 240

Query: 241 GSNGEE--LNSLFMEHVVEFVATDGREDESPISTLEVHHVGRTRYILSSDARGKIKVFRE 300
           GSNGE+   NS+ MEHV +F   D  +   P++ LEVHHVGR RYIL++D  GK+ V  E
Sbjct: 241 GSNGEDWNSNSVSMEHVGKFDVDDSAD---PVTLLEVHHVGRVRYILATDLSGKLTVLTE 300

Query: 301 NGTVYGSVMATSRPIAFLKQRLLFLTESGAGSLDLRSMKLRESECEGLNYSLARNYVFDA 360
           N TVYGSV+ +SRP+ FLKQRLLFLTESGAGSLDLRSMK+RE+ECEGLN+SLAR YVFDA
Sbjct: 301 NRTVYGSVIPSSRPLVFLKQRLLFLTESGAGSLDLRSMKIRETECEGLNHSLARTYVFDA 360

Query: 361 TERSKAYGLTSDGDLIHVLLLGDIMNFKCRVRSKRKFEFDEPLGFQAIKGYLLVISNEKV 420
            ERSKAYG TS+G++IHVLL GDIMNFKCRVRSK+KF+ +EP+  Q+IKGYLLVI+ EKV
Sbjct: 361 AERSKAYGFTSEGEIIHVLLHGDIMNFKCRVRSKKKFQMEEPVALQSIKGYLLVINEEKV 420

Query: 421 HIFNVSSQHYVRVGAPRHLFSAGLDEIRSSFLNNQNLD--LDSEGKFTPLISSDREKLVV 480
             FNVS+QHYVR   PR LFSAGL+EIRS+FL+++       +  K  PLI+SDRE L+V
Sbjct: 421 FAFNVSTQHYVRTAGPRLLFSAGLEEIRSAFLSHRESSSRTTTVVKTRPLIASDRENLLV 480

Query: 481 IGLGGGYVGMYRSNLPIFKGEFNTMLWTSPVLFFILFLFGAWQFFAKKKEALTSWGPDDP 540
           IGL  GY  +Y+S LP  KG+FNTMLW+SPV FFILFLFGAW FFAKKKE+LT+WGPDDP
Sbjct: 481 IGLENGYFAVYKSKLPTLKGDFNTMLWSSPVFFFILFLFGAWHFFAKKKESLTAWGPDDP 540

Query: 541 FTATSPTTGAPLVTGSSERASFIDTASRSTDVMDLRGGSGLRGPPRRYGSPTGYPGGATS 600
           FT     TGA     S++  +F + + R+ D+MDLR         RRY         A  
Sbjct: 541 FT----PTGAQ--NSSAKEPTFTEPSRRNDDLMDLR---------RRY---------AGG 600

Query: 601 SFRPATTSDHNSRPAAVDPNYRAASE----LKFRGSPLEPPGFPKRREPLFANNQVVN 647
           S+R    +D +SR A VD NYR  ++     +  GS L+  GF  RR+ LF NN+V++
Sbjct: 601 SYRSVGANDPSSR-APVDGNYRTTAQDHNNYRGGGSGLDSNGFGNRRDHLFGNNKVLD 602