Lag0004325 (gene) Sponge gourd (AG‐4) v1

Overview
NameLag0004325
Typegene
OrganismLuffa acutangula (Sponge gourd (AG‐4) v1)
Descriptionmitogen-activated protein kinase kinase kinase 5-like
Locationchr6: 2949106 .. 2952984 (-)
RNA-Seq ExpressionLag0004325
SyntenyLag0004325
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: polypeptideexonCDS
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGCCTTTCTTTTACAAAAGCGCTTCGAATTCTCGGATTCAACAGAAGGTTCCTGATTCTGAATTTTCCGTTGGTCTACCGGAACCGCTCGTTCAACGGCGGCTCACCCGACAGAGGAAGCTCCGGCATCTTACCGATCAAGACGTTGGCCTTCAACACCGCCGATCTTTTCCCGATTCCCCCGATTTATCGGCTAAACCTAGATCGCCTCTTGGGGGTTCCGAACGTTGGTCATCGGAGGCTTTGCCCCAGCCTCTGCCTCTGCCGGAGTACTTTCCGCTTCGAAATCCGGAGTCGGGTTCTTGGAGTTCCGGCCAAGGCCGCGTTGGATCGCCGATTGAAGCTCCGGTCCGGTGAGTTTTCTCTTGTTTTGACTTGAATTTTGCCCTAGGAATTGATGGAATTTTCGTGTAATTTCCTTTGATTTCTGTTATACTTTGATTCTGTATTGTAAAGGGGAAAATGAGGTTTTGACCTAATTTGTTTCTTGTTTGTAATTGTGTCGTTAAAACTGTTAGGAAGAGCTCTGATCATTCAACAACGAATGCAAATAGAAGTTTGGGCAACAATCAAAGGCGTGTTGCTATAGATTTTGCCTTGGAAGTTGCTAATGGCAATACAGGAGGGAATGGGGCTACCACATTTACAATCCCTCAAAGTCTCTCTGTGAACTCAGGGAAATCAAATTCGAATAAGGAGTTTGTTTTTGTTTGTTTGGATCAGCCTCCAAACAACAAAGGCAGGGGATCTCCTACTCATCCGAGTGTTGAAAGGGGCAACTTCAATTTGACACCGAAGACTTCGTCGTCGAAGAGTGCGCCAACGAGCATTTTACCGAGTCCCGCCGTTAGCCCACGAAGATCATATACTGGAGATCTTTTTGTTTCTGGTTTGACTCGCCATGAACATCAGGAATCTCCTTCTAATAATTCTCCCAAAGTACCAACCCTTAAAACTGCACATAGTTCTCAGCCTTCTCCTCTCCATAGCCCAACAACTCGAAGTTCCGCAAGCAATTCCAGATCTCCTAATGATATGCCATTTCCTCTGCATCATAAGTTACAAAAGGATAGCAGTATAGATCGGTCCGAAAACCATGGACATGTCAATGCCCATCCATTGCCCCTTCCTCCTCCATTAATAGCCTGTTCACAATCATCTCCACAATCTCATTCGTCGAATGTTCATCATGTTATTGAGAAACCATTCATTTCATCTATGAAATGTCAGTGGCAGAAAGGAAAGCTTATTGGGCGTGGTACGTTCGGAAGCGTTTATCTTGCAACCAACAGGTATGAATGGAAACTAAGCTTTGCACTATTGTTATAATTAAGCGTTTATGAAGTCTTCCTCATATTTGCTTGCATACTTTCCAGAGAGACTGGAGCTTTATGTGCAATGAAAGAAGTTGACCTCATTCCTGATGATCCTAAGAGTGCTGAAAGTATAAAGCAACTAGAGCAGGTATTAAGTCATCTACTTGAATTCTCATATAGCATTTATTTTCCTGTCAGTTTTTTTGGTTTATCCTAAAGATAGATGTCATTTACCAGCAACCTTCGAAACTCTTTTTCCTCGTCAATCGCTATTTAAGTCGTTATCGGGTTTCAAATTAGAAACTTGCTTAGTGGTGTACCGTACATATTCATTAGATTAGCTTTATCCAAATTATCCTATCCCTATAAACATGAGTAAGCCATCTATGGCTGTACAAATTGCTCTAAAGCTAGCCAAGTATTGCTTTCTGTGAATTTGAACGACCAAATGTATGAGTTTCTACTATTCTTCTTCTTATCATGTCACCTAGACAAACTCGAGCTTGCTTTTTACTGGCTTTGATGCTATTAACATTCGTCCTATGATGTTTTCCTGTCATCTGACTCCTTTATTGTCTCAGGAAATTAAAGTTCTCAGTCAGCTTAAACATCCAAATATTGTACAGTATTATGGAAGTGAAATAGTAAGTGTTCTGCCTTATCTTTTGTATCAGGAATTACTTCTACCCGTATTGACAACCCCAAAAATTTTGGTTCAACTTTTGGTATGTAGATTGATGATCATTTCTATATATATTTGGAGTACGTTTTTCCGGGTTCAATCAATAAATATGTCCGTGAACATTGTGGAGCTATTACAGAATCCATCGTTCGGAATTTCACTCGCCATATTCTCTCTGGACTGGCTTACTTGCATAGCACAAAAACAATCCACAGGTGCGAGACAAATTCTTTCTCCAAGATAAGATTTTTGTTCCCATTGCTGAAGACGAGACGAAATGATAAAAGAAAGTCATTGGATATGCTTGCTTTCTCTAAAAATAATATATACATTTATTTGTTTACTTCCACAATGTACGGCTTTGTGATTTTTTCTTTTTCTTTTTCTTTTTTTCATTTTATTCTGCTTTTTATAGGGATATCAAAGGTGCAAACTTGCTAGTTGACTCGTCAGGCGTTGTCAAACTTGCAGATTTTGGGATGGCAAAACATGTGAGTTTTCAAGTGCTAATGAATATCATCTTAATTGTTTACATTTTATGAACTTTGGTCATTGTATCCTCTTGAGAATTCTGAATATCTTGGAAGGGAATGAGATGTTGCAGATGCTTCAACTCTTACTGTTAACTATTTCAACTTGTGTGCTTTATTTTTCATAAATGTTCTACTATTTACCAATCTCTTGTTTCTCTACTCAGCTGACAGGGCAGTATGATCTTTCTTTGAAGGGTAGTCCGTACTGGATGGCTCCGGAGGTATGTCACGGACGGTCAACTTTATTTTTTAAACCGCACGTTCTCTTCTAATTCCACGAATTCGTTTTCAGGTCATAAAGGCTGCTATGTTGAAAGATATCAACCCTGATCTTGCTTTGGCAGTTGACATTTGGAGTCTGGGTTGCACCATCATTGAAATGCTGAATGGAAAACCTCCATGGAGTGAGTATGAAGGGGTGAGTTCGTTGTTTAGTTTTTGTTCTTTCTATCTTCTAGATCATTCAAAAAGAAAGGAAAATGAGTGTCTTTATTGTAAGGAACGAAAGAGATATCGACAATAATGTATGATTTTCTTTAGACAAAACTTAGCTGCTGACGAGCGAGCATTGGATACATGACATGACAAAGAAATTGAAACAAAATGGAGTCAGCAGCGAAGTTCTGTCCTCTTCCGAAACATCTTTCATTCAACTAACGATAAATTACATTTTCGACTTTCCAGCATCAAGCTATGTTCAAAGTGTTGAACAAAACCCCACCTATCCCAGAAAAGCTATCTCCAGAAGGGAAAGACTTCCTCCAGTGTTGCTTCCAAAGGAATCCAGCTGACAGACCAACAGCCATGGTGCTGCTTGACCATCCTTTCTTAAGATTCTCAAATGACTCAAATGTTTCAATCCCCACATCAGCATTTTCAACAATGAACCTCTTGGTTAGTTTTACTTTTAGAACATTAATTTTTTGGTTTTATGCCTAAAAACATCTCATTAATTTTCGCATTCTACGCTCTCTTAGGAAAAATTGTTGAGTCCCAAGGACCACCTTCATCCCAAAGGAGACCAAGGGCAAAACTCTCCCGGAACTTCGGCTGCAAATGATTACCTGCCGTGCCGTAGGTAGCTATACTAACTCTTGCTCATGCACTTATAAACTGTATGATGATGAAGCACAAGCTTGTGAATATTCTCTTCTGGTTTTTTTTTCTTCTTTATTTATCTGCGGTCTTGATGTCGATTCTTGTAAGCTTTCCTTACAACTGACCTCCGTTTTTAATCGAATCGTGTAGCCCTTCAACGAACATTCCAAGCAATATTCCTGCCAGTGCCGTTAACAACCATCACTTTGCTCTTTCAAGGACCCATACAAGGGAAGTGCCGCACCTCTGA

mRNA sequence

ATGCCTTTCTTTTACAAAAGCGCTTCGAATTCTCGGATTCAACAGAAGGTTCCTGATTCTGAATTTTCCGTTGGTCTACCGGAACCGCTCGTTCAACGGCGGCTCACCCGACAGAGGAAGCTCCGGCATCTTACCGATCAAGACGTTGGCCTTCAACACCGCCGATCTTTTCCCGATTCCCCCGATTTATCGGCTAAACCTAGATCGCCTCTTGGGGGTTCCGAACGTTGGTCATCGGAGGCTTTGCCCCAGCCTCTGCCTCTGCCGGAGTACTTTCCGCTTCGAAATCCGGAGTCGGGTTCTTGGAGTTCCGGCCAAGGCCGCGTTGGATCGCCGATTGAAGCTCCGGTCCGGAAGAGCTCTGATCATTCAACAACGAATGCAAATAGAAGTTTGGGCAACAATCAAAGGCGTGTTGCTATAGATTTTGCCTTGGAAGTTGCTAATGGCAATACAGGAGGGAATGGGGCTACCACATTTACAATCCCTCAAAGTCTCTCTGTGAACTCAGGGAAATCAAATTCGAATAAGGAGTTTGTTTTTGTTTGTTTGGATCAGCCTCCAAACAACAAAGGCAGGGGATCTCCTACTCATCCGAGTGTTGAAAGGGGCAACTTCAATTTGACACCGAAGACTTCGTCGTCGAAGAGTGCGCCAACGAGCATTTTACCGAGTCCCGCCGTTAGCCCACGAAGATCATATACTGGAGATCTTTTTGTTTCTGGTTTGACTCGCCATGAACATCAGGAATCTCCTTCTAATAATTCTCCCAAAGTACCAACCCTTAAAACTGCACATAGTTCTCAGCCTTCTCCTCTCCATAGCCCAACAACTCGAAGTTCCGCAAGCAATTCCAGATCTCCTAATGATATGCCATTTCCTCTGCATCATAAGTTACAAAAGGATAGCAGTATAGATCGGTCCGAAAACCATGGACATGTCAATGCCCATCCATTGCCCCTTCCTCCTCCATTAATAGCCTGTTCACAATCATCTCCACAATCTCATTCGTCGAATGTTCATCATGTTATTGAGAAACCATTCATTTCATCTATGAAATGTCAGTGGCAGAAAGGAAAGCTTATTGGGCGTGGTACGTTCGGAAGCGTTTATCTTGCAACCAACAGAGAGACTGGAGCTTTATGTGCAATGAAAGAAGTTGACCTCATTCCTGATGATCCTAAGAGTGCTGAAAGTATAAAGCAACTAGAGCAGGAAATTAAAGTTCTCAGTCAGCTTAAACATCCAAATATTGTACAGTATTATGGAAGTGAAATAATTGATGATCATTTCTATATATATTTGGAGTACGTTTTTCCGGGTTCAATCAATAAATATGTCCGTGAACATTGTGGAGCTATTACAGAATCCATCGTTCGGAATTTCACTCGCCATATTCTCTCTGGACTGGCTTACTTGCATAGCACAAAAACAATCCACAGGGATATCAAAGGTGCAAACTTGCTAGTTGACTCGTCAGGCGTTGTCAAACTTGCAGATTTTGGGATGGCAAAACATCTGACAGGGCAGTATGATCTTTCTTTGAAGGGTAGTCCGTACTGGATGGCTCCGGAGGTCATAAAGGCTGCTATGTTGAAAGATATCAACCCTGATCTTGCTTTGGCAGTTGACATTTGGAGTCTGGGTTGCACCATCATTGAAATGCTGAATGGAAAACCTCCATGGAGTGAGTATGAAGGGCATCAAGCTATGTTCAAAGTGTTGAACAAAACCCCACCTATCCCAGAAAAGCTATCTCCAGAAGGGAAAGACTTCCTCCAGTGTTGCTTCCAAAGGAATCCAGCTGACAGACCAACAGCCATGGTGCTGCTTGACCATCCTTTCTTAAGATTCTCAAATGACTCAAATGTTTCAATCCCCACATCAGCATTTTCAACAATGAACCTCTTGGAAAAATTGTTGAGTCCCAAGGACCACCTTCATCCCAAAGGAGACCAAGGGCAAAACTCTCCCGGAACTTCGGCTGCAAATGATTACCTGCCGTGCCGTAGCCCTTCAACGAACATTCCAAGCAATATTCCTGCCAGTGCCGTTAACAACCATCACTTTGCTCTTTCAAGGACCCATACAAGGGAAGTGCCGCACCTCTGA

Coding sequence (CDS)

ATGCCTTTCTTTTACAAAAGCGCTTCGAATTCTCGGATTCAACAGAAGGTTCCTGATTCTGAATTTTCCGTTGGTCTACCGGAACCGCTCGTTCAACGGCGGCTCACCCGACAGAGGAAGCTCCGGCATCTTACCGATCAAGACGTTGGCCTTCAACACCGCCGATCTTTTCCCGATTCCCCCGATTTATCGGCTAAACCTAGATCGCCTCTTGGGGGTTCCGAACGTTGGTCATCGGAGGCTTTGCCCCAGCCTCTGCCTCTGCCGGAGTACTTTCCGCTTCGAAATCCGGAGTCGGGTTCTTGGAGTTCCGGCCAAGGCCGCGTTGGATCGCCGATTGAAGCTCCGGTCCGGAAGAGCTCTGATCATTCAACAACGAATGCAAATAGAAGTTTGGGCAACAATCAAAGGCGTGTTGCTATAGATTTTGCCTTGGAAGTTGCTAATGGCAATACAGGAGGGAATGGGGCTACCACATTTACAATCCCTCAAAGTCTCTCTGTGAACTCAGGGAAATCAAATTCGAATAAGGAGTTTGTTTTTGTTTGTTTGGATCAGCCTCCAAACAACAAAGGCAGGGGATCTCCTACTCATCCGAGTGTTGAAAGGGGCAACTTCAATTTGACACCGAAGACTTCGTCGTCGAAGAGTGCGCCAACGAGCATTTTACCGAGTCCCGCCGTTAGCCCACGAAGATCATATACTGGAGATCTTTTTGTTTCTGGTTTGACTCGCCATGAACATCAGGAATCTCCTTCTAATAATTCTCCCAAAGTACCAACCCTTAAAACTGCACATAGTTCTCAGCCTTCTCCTCTCCATAGCCCAACAACTCGAAGTTCCGCAAGCAATTCCAGATCTCCTAATGATATGCCATTTCCTCTGCATCATAAGTTACAAAAGGATAGCAGTATAGATCGGTCCGAAAACCATGGACATGTCAATGCCCATCCATTGCCCCTTCCTCCTCCATTAATAGCCTGTTCACAATCATCTCCACAATCTCATTCGTCGAATGTTCATCATGTTATTGAGAAACCATTCATTTCATCTATGAAATGTCAGTGGCAGAAAGGAAAGCTTATTGGGCGTGGTACGTTCGGAAGCGTTTATCTTGCAACCAACAGAGAGACTGGAGCTTTATGTGCAATGAAAGAAGTTGACCTCATTCCTGATGATCCTAAGAGTGCTGAAAGTATAAAGCAACTAGAGCAGGAAATTAAAGTTCTCAGTCAGCTTAAACATCCAAATATTGTACAGTATTATGGAAGTGAAATAATTGATGATCATTTCTATATATATTTGGAGTACGTTTTTCCGGGTTCAATCAATAAATATGTCCGTGAACATTGTGGAGCTATTACAGAATCCATCGTTCGGAATTTCACTCGCCATATTCTCTCTGGACTGGCTTACTTGCATAGCACAAAAACAATCCACAGGGATATCAAAGGTGCAAACTTGCTAGTTGACTCGTCAGGCGTTGTCAAACTTGCAGATTTTGGGATGGCAAAACATCTGACAGGGCAGTATGATCTTTCTTTGAAGGGTAGTCCGTACTGGATGGCTCCGGAGGTCATAAAGGCTGCTATGTTGAAAGATATCAACCCTGATCTTGCTTTGGCAGTTGACATTTGGAGTCTGGGTTGCACCATCATTGAAATGCTGAATGGAAAACCTCCATGGAGTGAGTATGAAGGGCATCAAGCTATGTTCAAAGTGTTGAACAAAACCCCACCTATCCCAGAAAAGCTATCTCCAGAAGGGAAAGACTTCCTCCAGTGTTGCTTCCAAAGGAATCCAGCTGACAGACCAACAGCCATGGTGCTGCTTGACCATCCTTTCTTAAGATTCTCAAATGACTCAAATGTTTCAATCCCCACATCAGCATTTTCAACAATGAACCTCTTGGAAAAATTGTTGAGTCCCAAGGACCACCTTCATCCCAAAGGAGACCAAGGGCAAAACTCTCCCGGAACTTCGGCTGCAAATGATTACCTGCCGTGCCGTAGCCCTTCAACGAACATTCCAAGCAATATTCCTGCCAGTGCCGTTAACAACCATCACTTTGCTCTTTCAAGGACCCATACAAGGGAAGTGCCGCACCTCTGA

Protein sequence

MPFFYKSASNSRIQQKVPDSEFSVGLPEPLVQRRLTRQRKLRHLTDQDVGLQHRRSFPDSPDLSAKPRSPLGGSERWSSEALPQPLPLPEYFPLRNPESGSWSSGQGRVGSPIEAPVRKSSDHSTTNANRSLGNNQRRVAIDFALEVANGNTGGNGATTFTIPQSLSVNSGKSNSNKEFVFVCLDQPPNNKGRGSPTHPSVERGNFNLTPKTSSSKSAPTSILPSPAVSPRRSYTGDLFVSGLTRHEHQESPSNNSPKVPTLKTAHSSQPSPLHSPTTRSSASNSRSPNDMPFPLHHKLQKDSSIDRSENHGHVNAHPLPLPPPLIACSQSSPQSHSSNVHHVIEKPFISSMKCQWQKGKLIGRGTFGSVYLATNRETGALCAMKEVDLIPDDPKSAESIKQLEQEIKVLSQLKHPNIVQYYGSEIIDDHFYIYLEYVFPGSINKYVREHCGAITESIVRNFTRHILSGLAYLHSTKTIHRDIKGANLLVDSSGVVKLADFGMAKHLTGQYDLSLKGSPYWMAPEVIKAAMLKDINPDLALAVDIWSLGCTIIEMLNGKPPWSEYEGHQAMFKVLNKTPPIPEKLSPEGKDFLQCCFQRNPADRPTAMVLLDHPFLRFSNDSNVSIPTSAFSTMNLLEKLLSPKDHLHPKGDQGQNSPGTSAANDYLPCRSPSTNIPSNIPASAVNNHHFALSRTHTREVPHL
Homology
BLAST of Lag0004325 vs. NCBI nr
Match: XP_038885756.1 (mitogen-activated protein kinase kinase kinase 5-like isoform X2 [Benincasa hispida])

HSP 1 Score: 1214.1 bits (3140), Expect = 0.0e+00
Identity = 607/704 (86.22%), Postives = 646/704 (91.76%), Query Frame = 0

Query: 1   MPFFYKSASNSRIQQKVPDSEFSVGLPEPLVQRRLTRQRKLRHLTDQDVGLQHRRSFPDS 60
           MPFF+KSASNSR+QQK+PDSEFSV LPEPLVQRRLTRQRKLRHLTD+DVG ++ RS PDS
Sbjct: 1   MPFFHKSASNSRLQQKLPDSEFSVVLPEPLVQRRLTRQRKLRHLTDRDVGFEYPRSSPDS 60

Query: 61  PDLSAKPRSPLGGSERWSSEALPQPLPLPEYFPLRNPESGSWSSGQGRVGSPIEAPVRKS 120
           PDL AKPRSPLGGSERWSS  LPQPLPLPE+FP+R+ ESGSWSSG GRVGSP+E PVRKS
Sbjct: 61  PDLLAKPRSPLGGSERWSSSPLPQPLPLPEFFPVRSLESGSWSSGHGRVGSPVENPVRKS 120

Query: 121 SDHSTTNANRSLGNNQRRVAIDFALEVANGNTGGNGATTFTIPQSLSVNSGKSNSNKEFV 180
           SDH TTN +R LG+NQRRVA+D  LEV NGN+GG GATTFT  QS SVNSGKSN NKE +
Sbjct: 121 SDHGTTNVSRILGDNQRRVAVDLTLEVVNGNSGGTGATTFTSLQSPSVNSGKSNPNKELL 180

Query: 181 FVCLDQPPNNKGRGSPTHPSVERGNFNLTPKTSSSKSAPTSILPSPAVSPRRSYTGDLFV 240
           F CLDQPPNNKGRGSPT+PS ERGN+N TPK SSSKSAPTSILPSPAVSPRR+YTG+ FV
Sbjct: 181 FGCLDQPPNNKGRGSPTYPSNERGNYNSTPKNSSSKSAPTSILPSPAVSPRRTYTGNPFV 240

Query: 241 SGLTRHEHQESPSNNSPKVPTLKTAHSSQPSPLHSPTTRSSASNSRSPNDMPFPLHHK-L 300
           SGLT HE+QESPSNNSPKVP LKTA +SQ SPL+SPT +SS SN RSPNDM FPLH K L
Sbjct: 241 SGLTHHEYQESPSNNSPKVPPLKTALTSQHSPLYSPTAQSSVSNPRSPNDMAFPLHSKLL 300

Query: 301 QKDSSIDRSENHGHVNAHPLPLPPPLIACSQSSPQSHSSNVHHVIEKPFISSMKCQWQKG 360
            K+SSIDR+E+HGHVNAHPLPLPPPL+A SQ+SPQS  S+VHHVIEKP+ SSMK QWQKG
Sbjct: 301 LKNSSIDRAESHGHVNAHPLPLPPPLVAPSQASPQSIPSSVHHVIEKPYTSSMKGQWQKG 360

Query: 361 KLIGRGTFGSVYLATNRETGALCAMKEVDLIPDDPKSAESIKQLEQEIKVLSQLKHPNIV 420
           KLIGRGTFGSVYLATNRETGALCAMKEVDLIPDDPKSAE IKQLEQEIKVLS LKHPNIV
Sbjct: 361 KLIGRGTFGSVYLATNRETGALCAMKEVDLIPDDPKSAECIKQLEQEIKVLSHLKHPNIV 420

Query: 421 QYYGSEIIDDHFYIYLEYVFPGSINKYVREHCGAITESIVRNFTRHILSGLAYLHSTKTI 480
           QYYGSE I DHFYIYLEYV+PGSINKYVRE CGAITESIVRNFTRHILSGLAYLHSTKTI
Sbjct: 421 QYYGSETIGDHFYIYLEYVYPGSINKYVRERCGAITESIVRNFTRHILSGLAYLHSTKTI 480

Query: 481 HRDIKGANLLVDSSGVVKLADFGMAKHLTGQYDLSLKGSPYWMAPEVIKAAMLKDINPDL 540
           HRDIKGANLLVDSSGVVKLADFGMAKHLTGQYDLSLKGSPYWMAPEVIKAAMLKD NPDL
Sbjct: 481 HRDIKGANLLVDSSGVVKLADFGMAKHLTGQYDLSLKGSPYWMAPEVIKAAMLKDANPDL 540

Query: 541 ALAVDIWSLGCTIIEMLNGKPPWSEYEGHQAMFKVLNKTPPIPEKLSPEGKDFLQCCFQR 600
           ALAVDIWSLGCTIIEMLNGKPPW E+EGHQAMFKVLNKTP IPEKLSPEGKDFLQCCFQR
Sbjct: 541 ALAVDIWSLGCTIIEMLNGKPPWCEFEGHQAMFKVLNKTPAIPEKLSPEGKDFLQCCFQR 600

Query: 601 NPADRPTAMVLLDHPFLRFSNDSNVSIPTSAFSTMNLLEKLLSPKDHLHPKGDQGQNSPG 660
           NPADRPTAMVLLDHPFLR SNDSNVSIPTS+FSTMNLLEKL SPKD L+PKGDQ QNSPG
Sbjct: 601 NPADRPTAMVLLDHPFLRSSNDSNVSIPTSSFSTMNLLEKLSSPKDPLNPKGDQAQNSPG 660

Query: 661 TSAANDYLPCRSPSTNIPSNIPASAVNNHHFALSRTHTREVPHL 704
           TSA+N+YLPCRSPSTNI SN+PASAV NHHF++SRTH REVPHL
Sbjct: 661 TSASNNYLPCRSPSTNISSNVPASAVTNHHFSISRTHKREVPHL 704

BLAST of Lag0004325 vs. NCBI nr
Match: XP_038885754.1 (mitogen-activated protein kinase kinase kinase 5-like isoform X1 [Benincasa hispida])

HSP 1 Score: 1206.8 bits (3121), Expect = 0.0e+00
Identity = 607/712 (85.25%), Postives = 646/712 (90.73%), Query Frame = 0

Query: 1   MPFFYKSASNSRIQQKVPDSEFSVGLPEPLVQRRLTRQRKLRHLTDQDVGLQHRRSFPDS 60
           MPFF+KSASNSR+QQK+PDSEFSV LPEPLVQRRLTRQRKLRHLTD+DVG ++ RS PDS
Sbjct: 1   MPFFHKSASNSRLQQKLPDSEFSVVLPEPLVQRRLTRQRKLRHLTDRDVGFEYPRSSPDS 60

Query: 61  PDLSAKPRSPLGGSERWSSEALPQPLPLPEYFPLRNPESGSWSSGQGRVGSPIEAP---- 120
           PDL AKPRSPLGGSERWSS  LPQPLPLPE+FP+R+ ESGSWSSG GRVGSP+E P    
Sbjct: 61  PDLLAKPRSPLGGSERWSSSPLPQPLPLPEFFPVRSLESGSWSSGHGRVGSPVENPVRNS 120

Query: 121 ----VRKSSDHSTTNANRSLGNNQRRVAIDFALEVANGNTGGNGATTFTIPQSLSVNSGK 180
               VRKSSDH TTN +R LG+NQRRVA+D  LEV NGN+GG GATTFT  QS SVNSGK
Sbjct: 121 AIKTVRKSSDHGTTNVSRILGDNQRRVAVDLTLEVVNGNSGGTGATTFTSLQSPSVNSGK 180

Query: 181 SNSNKEFVFVCLDQPPNNKGRGSPTHPSVERGNFNLTPKTSSSKSAPTSILPSPAVSPRR 240
           SN NKE +F CLDQPPNNKGRGSPT+PS ERGN+N TPK SSSKSAPTSILPSPAVSPRR
Sbjct: 181 SNPNKELLFGCLDQPPNNKGRGSPTYPSNERGNYNSTPKNSSSKSAPTSILPSPAVSPRR 240

Query: 241 SYTGDLFVSGLTRHEHQESPSNNSPKVPTLKTAHSSQPSPLHSPTTRSSASNSRSPNDMP 300
           +YTG+ FVSGLT HE+QESPSNNSPKVP LKTA +SQ SPL+SPT +SS SN RSPNDM 
Sbjct: 241 TYTGNPFVSGLTHHEYQESPSNNSPKVPPLKTALTSQHSPLYSPTAQSSVSNPRSPNDMA 300

Query: 301 FPLHHK-LQKDSSIDRSENHGHVNAHPLPLPPPLIACSQSSPQSHSSNVHHVIEKPFISS 360
           FPLH K L K+SSIDR+E+HGHVNAHPLPLPPPL+A SQ+SPQS  S+VHHVIEKP+ SS
Sbjct: 301 FPLHSKLLLKNSSIDRAESHGHVNAHPLPLPPPLVAPSQASPQSIPSSVHHVIEKPYTSS 360

Query: 361 MKCQWQKGKLIGRGTFGSVYLATNRETGALCAMKEVDLIPDDPKSAESIKQLEQEIKVLS 420
           MK QWQKGKLIGRGTFGSVYLATNRETGALCAMKEVDLIPDDPKSAE IKQLEQEIKVLS
Sbjct: 361 MKGQWQKGKLIGRGTFGSVYLATNRETGALCAMKEVDLIPDDPKSAECIKQLEQEIKVLS 420

Query: 421 QLKHPNIVQYYGSEIIDDHFYIYLEYVFPGSINKYVREHCGAITESIVRNFTRHILSGLA 480
            LKHPNIVQYYGSE I DHFYIYLEYV+PGSINKYVRE CGAITESIVRNFTRHILSGLA
Sbjct: 421 HLKHPNIVQYYGSETIGDHFYIYLEYVYPGSINKYVRERCGAITESIVRNFTRHILSGLA 480

Query: 481 YLHSTKTIHRDIKGANLLVDSSGVVKLADFGMAKHLTGQYDLSLKGSPYWMAPEVIKAAM 540
           YLHSTKTIHRDIKGANLLVDSSGVVKLADFGMAKHLTGQYDLSLKGSPYWMAPEVIKAAM
Sbjct: 481 YLHSTKTIHRDIKGANLLVDSSGVVKLADFGMAKHLTGQYDLSLKGSPYWMAPEVIKAAM 540

Query: 541 LKDINPDLALAVDIWSLGCTIIEMLNGKPPWSEYEGHQAMFKVLNKTPPIPEKLSPEGKD 600
           LKD NPDLALAVDIWSLGCTIIEMLNGKPPW E+EGHQAMFKVLNKTP IPEKLSPEGKD
Sbjct: 541 LKDANPDLALAVDIWSLGCTIIEMLNGKPPWCEFEGHQAMFKVLNKTPAIPEKLSPEGKD 600

Query: 601 FLQCCFQRNPADRPTAMVLLDHPFLRFSNDSNVSIPTSAFSTMNLLEKLLSPKDHLHPKG 660
           FLQCCFQRNPADRPTAMVLLDHPFLR SNDSNVSIPTS+FSTMNLLEKL SPKD L+PKG
Sbjct: 601 FLQCCFQRNPADRPTAMVLLDHPFLRSSNDSNVSIPTSSFSTMNLLEKLSSPKDPLNPKG 660

Query: 661 DQGQNSPGTSAANDYLPCRSPSTNIPSNIPASAVNNHHFALSRTHTREVPHL 704
           DQ QNSPGTSA+N+YLPCRSPSTNI SN+PASAV NHHF++SRTH REVPHL
Sbjct: 661 DQAQNSPGTSASNNYLPCRSPSTNISSNVPASAVTNHHFSISRTHKREVPHL 712

BLAST of Lag0004325 vs. NCBI nr
Match: XP_008444652.1 (PREDICTED: mitogen-activated protein kinase kinase kinase YODA-like isoform X2 [Cucumis melo])

HSP 1 Score: 1198.0 bits (3098), Expect = 0.0e+00
Identity = 599/709 (84.49%), Postives = 635/709 (89.56%), Query Frame = 0

Query: 1   MPFFYKSASNSRIQQKVPD------SEFSVGLPEPLVQRRLTRQRKLRHLTDQDVGLQHR 60
           MPFF+KSASNSR+QQKVPD      S+FSVGLPEP VQRRLTRQRKLRHLTD+DVG +  
Sbjct: 1   MPFFHKSASNSRLQQKVPDSDFSDFSDFSVGLPEPFVQRRLTRQRKLRHLTDRDVGFEQP 60

Query: 61  RSFPDSPDLSAKPRSPLGGSERWSSEALPQPLPLPEYFPLRNPESGSWSSGQGRVGSPIE 120
           RS PDSPD+SAK +SPLGGSERWSS   PQPLPLPE FP+R+PESG W+S QGRVGSP E
Sbjct: 61  RSLPDSPDMSAKSKSPLGGSERWSSSPSPQPLPLPELFPVRSPESGCWNSVQGRVGSPDE 120

Query: 121 APVRKSSDHSTTNANRSLGNNQRRVAIDFALEVANGNTGGNGATTFTIPQSLSVNSGKSN 180
            PVRK SDH+TTN +RS G+N+RRVA D  LEV NGN+ G  ATTFT PQS SVNSGK N
Sbjct: 121 TPVRKCSDHATTNVSRSFGHNERRVATDLTLEVVNGNSRGTAATTFTSPQSFSVNSGKLN 180

Query: 181 SNKEFVFVCLDQPPNNKGRGSPTHPSVERGNFNLTPKTSSSKSAPTSILPSPAVSPRRSY 240
            NKEF+F CLDQ PNN GRGSPTHPS ERGN+NLT K SSSKSAPTSILPSP VSPR SY
Sbjct: 181 LNKEFLFGCLDQTPNNNGRGSPTHPSTERGNYNLTSKNSSSKSAPTSILPSPVVSPRTSY 240

Query: 241 TGDLFVSGLTRHEHQESPSNNSPKVPTLKTAHSSQPSPLHSPTTRSSASNSRSPNDMPFP 300
            G+  VSGLT HE+QESPSNNSPKVP LKTA SSQP PLHSPT RSS SNSR PNDM FP
Sbjct: 241 NGNHVVSGLTHHEYQESPSNNSPKVPPLKTALSSQPFPLHSPTARSSISNSRPPNDMTFP 300

Query: 301 LHHKLQKDSSIDRSENHGHVNAHPLPLPPPLIACSQSSPQSHSSNVHHVIEKPFISSMKC 360
           LH KLQKD+SIDRSE+H HVNAHPLPLPPPL+A SQ+SPQS  SNVHHVIEKP+ISSMK 
Sbjct: 301 LHPKLQKDNSIDRSESHAHVNAHPLPLPPPLVASSQASPQSLPSNVHHVIEKPYISSMKG 360

Query: 361 QWQKGKLIGRGTFGSVYLATNRETGALCAMKEVDLIPDDPKSAESIKQLEQEIKVLSQLK 420
           QWQKGKLIGRGTFGSVYLATNRETGALCAMKEVDLIPDDPKSAE IKQLEQEI+VLS LK
Sbjct: 361 QWQKGKLIGRGTFGSVYLATNRETGALCAMKEVDLIPDDPKSAECIKQLEQEIEVLSHLK 420

Query: 421 HPNIVQYYGSEIIDDHFYIYLEYVFPGSINKYVREHCGAITESIVRNFTRHILSGLAYLH 480
           HPNIVQYYGSEII D+FYIYLEYV+PGSINKYVREHCGAITESIVRNFTRHILSGLAYLH
Sbjct: 421 HPNIVQYYGSEIIGDYFYIYLEYVYPGSINKYVREHCGAITESIVRNFTRHILSGLAYLH 480

Query: 481 STKTIHRDIKGANLLVDSSGVVKLADFGMAKHLTGQYDLSLKGSPYWMAPEVIKAAMLKD 540
           STKTIHRDIKGANLLVDSSG VKLADFGMAKHLTGQYDLSLKGSPYWMAPEVIKAAMLKD
Sbjct: 481 STKTIHRDIKGANLLVDSSGAVKLADFGMAKHLTGQYDLSLKGSPYWMAPEVIKAAMLKD 540

Query: 541 INPDLALAVDIWSLGCTIIEMLNGKPPWSEYEGHQAMFKVLNKTPPIPEKLSPEGKDFLQ 600
            NPDLALAVDIWSLGCTIIEMLNGKPPW E+EGHQAMFKVLNKTPPIPEKLSPEGKDFLQ
Sbjct: 541 ANPDLALAVDIWSLGCTIIEMLNGKPPWCEFEGHQAMFKVLNKTPPIPEKLSPEGKDFLQ 600

Query: 601 CCFQRNPADRPTAMVLLDHPFLRFSNDSNVSIPTSAFSTMNLLEKLLSPKDHLHPKGDQG 660
           CCFQRNPADRP+A+VLLDHPFLR S+DSNVSIPTSAFSTM LLEKLL+PKD L+PKGDQ 
Sbjct: 601 CCFQRNPADRPSAVVLLDHPFLRSSSDSNVSIPTSAFSTMKLLEKLLNPKDPLNPKGDQA 660

Query: 661 QNSPGTSAANDYLPCRSPSTNIPSNIPASAVNNHHFALSRTHTREVPHL 704
           QNSPGTS +NDYL CRSPSTNI SNIPASAV NHHF++SRTH REVPHL
Sbjct: 661 QNSPGTSTSNDYLTCRSPSTNISSNIPASAVVNHHFSISRTHKREVPHL 709

BLAST of Lag0004325 vs. NCBI nr
Match: XP_008444651.1 (PREDICTED: mitogen-activated protein kinase kinase kinase YODA-like isoform X1 [Cucumis melo])

HSP 1 Score: 1190.6 bits (3079), Expect = 0.0e+00
Identity = 599/717 (83.54%), Postives = 635/717 (88.56%), Query Frame = 0

Query: 1   MPFFYKSASNSRIQQKVPD------SEFSVGLPEPLVQRRLTRQRKLRHLTDQDVGLQHR 60
           MPFF+KSASNSR+QQKVPD      S+FSVGLPEP VQRRLTRQRKLRHLTD+DVG +  
Sbjct: 1   MPFFHKSASNSRLQQKVPDSDFSDFSDFSVGLPEPFVQRRLTRQRKLRHLTDRDVGFEQP 60

Query: 61  RSFPDSPDLSAKPRSPLGGSERWSSEALPQPLPLPEYFPLRNPESGSWSSGQGRVGSPIE 120
           RS PDSPD+SAK +SPLGGSERWSS   PQPLPLPE FP+R+PESG W+S QGRVGSP E
Sbjct: 61  RSLPDSPDMSAKSKSPLGGSERWSSSPSPQPLPLPELFPVRSPESGCWNSVQGRVGSPDE 120

Query: 121 AP--------VRKSSDHSTTNANRSLGNNQRRVAIDFALEVANGNTGGNGATTFTIPQSL 180
            P        VRK SDH+TTN +RS G+N+RRVA D  LEV NGN+ G  ATTFT PQS 
Sbjct: 121 TPVRNSAIKTVRKCSDHATTNVSRSFGHNERRVATDLTLEVVNGNSRGTAATTFTSPQSF 180

Query: 181 SVNSGKSNSNKEFVFVCLDQPPNNKGRGSPTHPSVERGNFNLTPKTSSSKSAPTSILPSP 240
           SVNSGK N NKEF+F CLDQ PNN GRGSPTHPS ERGN+NLT K SSSKSAPTSILPSP
Sbjct: 181 SVNSGKLNLNKEFLFGCLDQTPNNNGRGSPTHPSTERGNYNLTSKNSSSKSAPTSILPSP 240

Query: 241 AVSPRRSYTGDLFVSGLTRHEHQESPSNNSPKVPTLKTAHSSQPSPLHSPTTRSSASNSR 300
            VSPR SY G+  VSGLT HE+QESPSNNSPKVP LKTA SSQP PLHSPT RSS SNSR
Sbjct: 241 VVSPRTSYNGNHVVSGLTHHEYQESPSNNSPKVPPLKTALSSQPFPLHSPTARSSISNSR 300

Query: 301 SPNDMPFPLHHKLQKDSSIDRSENHGHVNAHPLPLPPPLIACSQSSPQSHSSNVHHVIEK 360
            PNDM FPLH KLQKD+SIDRSE+H HVNAHPLPLPPPL+A SQ+SPQS  SNVHHVIEK
Sbjct: 301 PPNDMTFPLHPKLQKDNSIDRSESHAHVNAHPLPLPPPLVASSQASPQSLPSNVHHVIEK 360

Query: 361 PFISSMKCQWQKGKLIGRGTFGSVYLATNRETGALCAMKEVDLIPDDPKSAESIKQLEQE 420
           P+ISSMK QWQKGKLIGRGTFGSVYLATNRETGALCAMKEVDLIPDDPKSAE IKQLEQE
Sbjct: 361 PYISSMKGQWQKGKLIGRGTFGSVYLATNRETGALCAMKEVDLIPDDPKSAECIKQLEQE 420

Query: 421 IKVLSQLKHPNIVQYYGSEIIDDHFYIYLEYVFPGSINKYVREHCGAITESIVRNFTRHI 480
           I+VLS LKHPNIVQYYGSEII D+FYIYLEYV+PGSINKYVREHCGAITESIVRNFTRHI
Sbjct: 421 IEVLSHLKHPNIVQYYGSEIIGDYFYIYLEYVYPGSINKYVREHCGAITESIVRNFTRHI 480

Query: 481 LSGLAYLHSTKTIHRDIKGANLLVDSSGVVKLADFGMAKHLTGQYDLSLKGSPYWMAPEV 540
           LSGLAYLHSTKTIHRDIKGANLLVDSSG VKLADFGMAKHLTGQYDLSLKGSPYWMAPEV
Sbjct: 481 LSGLAYLHSTKTIHRDIKGANLLVDSSGAVKLADFGMAKHLTGQYDLSLKGSPYWMAPEV 540

Query: 541 IKAAMLKDINPDLALAVDIWSLGCTIIEMLNGKPPWSEYEGHQAMFKVLNKTPPIPEKLS 600
           IKAAMLKD NPDLALAVDIWSLGCTIIEMLNGKPPW E+EGHQAMFKVLNKTPPIPEKLS
Sbjct: 541 IKAAMLKDANPDLALAVDIWSLGCTIIEMLNGKPPWCEFEGHQAMFKVLNKTPPIPEKLS 600

Query: 601 PEGKDFLQCCFQRNPADRPTAMVLLDHPFLRFSNDSNVSIPTSAFSTMNLLEKLLSPKDH 660
           PEGKDFLQCCFQRNPADRP+A+VLLDHPFLR S+DSNVSIPTSAFSTM LLEKLL+PKD 
Sbjct: 601 PEGKDFLQCCFQRNPADRPSAVVLLDHPFLRSSSDSNVSIPTSAFSTMKLLEKLLNPKDP 660

Query: 661 LHPKGDQGQNSPGTSAANDYLPCRSPSTNIPSNIPASAVNNHHFALSRTHTREVPHL 704
           L+PKGDQ QNSPGTS +NDYL CRSPSTNI SNIPASAV NHHF++SRTH REVPHL
Sbjct: 661 LNPKGDQAQNSPGTSTSNDYLTCRSPSTNISSNIPASAVVNHHFSISRTHKREVPHL 717

BLAST of Lag0004325 vs. NCBI nr
Match: XP_022961725.1 (mitogen-activated protein kinase kinase kinase 5-like [Cucurbita moschata])

HSP 1 Score: 1182.2 bits (3057), Expect = 0.0e+00
Identity = 598/704 (84.94%), Postives = 627/704 (89.06%), Query Frame = 0

Query: 1   MPFFYKSASNSRIQQKVPDSEFSVGLPEPLVQRRLTRQRKLRHLTDQDVGLQHRRSFPDS 60
           MPFF+KSASNSR+QQK  DS FSV   E LVQRRLTRQRKLRHLTD+++G++  RSFPDS
Sbjct: 1   MPFFHKSASNSRLQQKAADSNFSVVQTESLVQRRLTRQRKLRHLTDREIGVELPRSFPDS 60

Query: 61  PDLSAKPRSPLGGSERWSSEALPQPLPLPEYFPLRNPESGSWSSGQGRVGSPIEAPVRKS 120
           PDLSAKPRSPLGGSERWSS ALPQPLPLPE FP+RNPES SW SGQGRV SPIE PVRKS
Sbjct: 61  PDLSAKPRSPLGGSERWSSPALPQPLPLPELFPVRNPESDSWFSGQGRVVSPIETPVRKS 120

Query: 121 SDHSTTNANRSLGNNQRRVAIDFALEVANGNTGGNGATTFTIPQSLSVNSGKSNSNKEFV 180
           SD +TTN N  LGNNQRRVA D  LEV NGNTGG GATTFT P S SVNSGKS S KEFV
Sbjct: 121 SDRATTNVNGGLGNNQRRVATDCKLEVVNGNTGGTGATTFTSPHSPSVNSGKSLSIKEFV 180

Query: 181 FVCLDQPPNNKGRGSPTHPSVERGNFNLTPKTSSSKSAPTSILPSPAVSPRRSYTGDLFV 240
           F CLDQPPN+K RGSPTHPS++RGN++LTPKTSSSKSAPTSILPSP  SPRRSYTGDLFV
Sbjct: 181 FGCLDQPPNSKVRGSPTHPSIQRGNYSLTPKTSSSKSAPTSILPSPTASPRRSYTGDLFV 240

Query: 241 SGLTRHEHQESPSNNSPKVPTLKTAHSSQPSPLHSPTTRSSASNSRSPNDMPFPLHHKLQ 300
           SGL RHE+QESP NNSPK P LKT H+SQPSPLHSP+ RS  S  +SPND+ FP H KLQ
Sbjct: 241 SGLPRHEYQESPFNNSPKFPPLKTPHTSQPSPLHSPSARSFVSGPKSPNDVKFPTHSKLQ 300

Query: 301 KD-SSIDRSENHGHVNAHPLPLPPPLIACSQSSPQSHSSNVHHVIEKPFISSMKCQWQKG 360
           KD SS DRSEN GHVNAHPLPLPPPL   SQSSP S SS+VH V EKP+ISS K +WQKG
Sbjct: 301 KDCSSTDRSENLGHVNAHPLPLPPPLAHSSQSSPLSLSSSVHRVNEKPYISSTKSRWQKG 360

Query: 361 KLIGRGTFGSVYLATNRETGALCAMKEVDLIPDDPKSAESIKQLEQEIKVLSQLKHPNIV 420
           KLIGRGTFGSVYLATN ETGALCAMKEVDLIPDDPK AESIKQLEQEIKVLSQLKHPNIV
Sbjct: 361 KLIGRGTFGSVYLATNSETGALCAMKEVDLIPDDPKCAESIKQLEQEIKVLSQLKHPNIV 420

Query: 421 QYYGSEIIDDHFYIYLEYVFPGSINKYVREHCGAITESIVRNFTRHILSGLAYLHSTKTI 480
           QYYGSEIIDDHFYIYLEYV+PGSINKYVRE CGAITESIVRNFTRHILSGLAYLHSTKTI
Sbjct: 421 QYYGSEIIDDHFYIYLEYVYPGSINKYVRERCGAITESIVRNFTRHILSGLAYLHSTKTI 480

Query: 481 HRDIKGANLLVDSSGVVKLADFGMAKHLTGQYDLSLKGSPYWMAPEVIKAAMLKDINPDL 540
           HRDIKGANLLVDSSGVVKLADFGMAKHLTGQYDLSLKGSPYWMAPEV+KAAMLKD NP+L
Sbjct: 481 HRDIKGANLLVDSSGVVKLADFGMAKHLTGQYDLSLKGSPYWMAPEVMKAAMLKDANPNL 540

Query: 541 ALAVDIWSLGCTIIEMLNGKPPWSEYEGHQAMFKVLNKTPPIPEKLSPEGKDFLQCCFQR 600
           ALAVDIWSLGCTIIEML+GKPPWSE+EGHQAMFKVLNKTPPIPEKLSPEGKDFLQCCFQR
Sbjct: 541 ALAVDIWSLGCTIIEMLDGKPPWSEFEGHQAMFKVLNKTPPIPEKLSPEGKDFLQCCFQR 600

Query: 601 NPADRPTAMVLLDHPFLRFSNDSNVSIPTSAFSTMNLLEKLLSPKDHLHPKGDQGQNSPG 660
           NPADRPTAM LLDHPFLR SNDSNVS PTSAFSTMN +EKLLSPKDH  PKGDQ QNS G
Sbjct: 601 NPADRPTAMALLDHPFLRSSNDSNVSTPTSAFSTMNRMEKLLSPKDHRSPKGDQAQNSSG 660

Query: 661 TSAANDYLPCRSPSTNIPSNIPASAVNNHHFALSRTHTREVPHL 704
           T A N YL CRSPSTNI SNIPASAVNNH FA+SRTH REVPHL
Sbjct: 661 TLAENGYLSCRSPSTNISSNIPASAVNNHPFAISRTHKREVPHL 704

BLAST of Lag0004325 vs. ExPASy Swiss-Prot
Match: Q9C5H5 (Mitogen-activated protein kinase kinase kinase 5 OS=Arabidopsis thaliana OX=3702 GN=MAPKKK5 PE=1 SV=1)

HSP 1 Score: 501.1 bits (1289), Expect = 2.0e-140
Identity = 328/688 (47.67%), Postives = 401/688 (58.28%), Query Frame = 0

Query: 34  RLTRQRKLRHLTDQDVGLQHRRSFPDSPD-----------LSAKPRSPLGGSERWSSEAL 93
           RLTRQRKLRHLTD DV L  RR+   S              +A PRSP       S+  L
Sbjct: 52  RLTRQRKLRHLTDDDVLLGERRASTSSSTFDSGLTRSPSAFTAVPRSP-------SAVPL 111

Query: 94  PQPLPLPEYFPLRNPESGSWSSGQGRVGSPIEAPVRKSSDHSTTNANRSLGNNQRRVAID 153
           P PLPLPE   +RN  +      + R    + +  R SS    T+ N     + R+   +
Sbjct: 112 PLPLPLPEVAGIRNAANARGLDDRDRDPERLISD-RTSSGPPLTSVNGGFARDSRKATEN 171

Query: 154 FALE---VANGNTGGNGATTFTIPQSLSVNSGKSNSNKEFVFVCLDQPPNNKGRGSPTHP 213
            + +     N N       T + P S  ++   S              P  K  G     
Sbjct: 172 SSYQDFSPRNRNGYWVNIPTMSAPTSPYMSPVPS--------------PQRKSTGH---- 231

Query: 214 SVERGNFNLTPKTSSSKSAPTSILPSPAVSPRRSYTGDLFVSGLTRHEHQESPSNNSPKV 273
             +   F L PK++ + SAP   L +  + P   Y    F    T +    SP   SP+ 
Sbjct: 232 --DLPFFYLPPKSNQAWSAPDMPLDTSGLPPPAFYDITAF---STDNSPIHSPQPRSPR- 291

Query: 274 PTLKTAHSSQPSPLHSPTTRSSASNSRSPND-MPFPLHHKLQKDSSIDRSENHGHVNAHP 333
              K   S QPS   SP    S  +S  P D +  PLH +L  D +  R +     N HP
Sbjct: 292 ---KQIRSPQPSRPSSPL--HSVDSSAPPRDSVSSPLHPRLSTDVTNGRRD---CCNVHP 351

Query: 334 LPLPPPLIACSQSSPQSHSSNVHHVIEKPFISSMKCQWQKGKLIGRGTFGSVYLATNRET 393
           LPLPP    CS SS  S  S    +    F   M  QW+KGKLIGRGTFGSVY+A+N ET
Sbjct: 352 LPLPPG-ATCSSSSAASVPSPQAPLKLDSF--PMNSQWKKGKLIGRGTFGSVYVASNSET 411

Query: 394 GALCAMKEVDLIPDDPKSAESIKQLEQEIKVLSQLKHPNIVQYYGSEIIDDHFYIYLEYV 453
           GALCAMKEV+L PDDPKSAE IKQLEQEIK+LS L+HPNIVQY+GSE ++D F+IYLEYV
Sbjct: 412 GALCAMKEVELFPDDPKSAECIKQLEQEIKLLSNLQHPNIVQYFGSETVEDRFFIYLEYV 471

Query: 454 FPGSINKYVREHCGAITESIVRNFTRHILSGLAYLHSTKTIHRDIKGANLLVDSSGVVKL 513
            PGSINKY+R+HCG +TES+VRNFTRHILSGLAYLH+ KT+HRDIKGANLLVD+SGVVKL
Sbjct: 472 HPGSINKYIRDHCGTMTESVVRNFTRHILSGLAYLHNKKTVHRDIKGANLLVDASGVVKL 531

Query: 514 ADFGMAKHLTGQ-YDLSLKGSPYWMAPEVIKAAMLKDINPDLALAVDIWSLGCTIIEMLN 573
           ADFGMAKHLTGQ  DLSLKGSPYWMAPE+++A M KD NPDLA AVDIWSLGCTIIEM  
Sbjct: 532 ADFGMAKHLTGQRADLSLKGSPYWMAPELMQAVMQKDSNPDLAFAVDIWSLGCTIIEMFT 591

Query: 574 GKPPWSEYEGHQAMFKVLNKTPPIPEKLSPEGKDFLQCCFQRNPADRPTAMVLLDHPFLR 633
           GKPPWSE+EG  AMFKV+  +PPIPE +SPEGKDFL+ CFQRNPA+RPTA +LL+H FL+
Sbjct: 592 GKPPWSEFEGAAAMFKVMRDSPPIPESMSPEGKDFLRLCFQRNPAERPTASMLLEHRFLK 651

Query: 634 FS---------------NDSNVSIPTSAFSTMNL-LEKLLSPKDHLHPKGDQGQ------ 683
            S               N  N++ P+S     N  L+++   ++    + + GQ      
Sbjct: 652 NSLQPTSPSNSDVSQLFNGMNITEPSSRREKPNFKLDQVPRARNMTSSESESGQQQQQQQ 696

BLAST of Lag0004325 vs. ExPASy Swiss-Prot
Match: Q9CAD5 (Mitogen-activated protein kinase kinase kinase YODA OS=Arabidopsis thaliana OX=3702 GN=YDA PE=1 SV=1)

HSP 1 Score: 339.3 bits (869), Expect = 1.0e-91
Identity = 267/679 (39.32%), Postives = 352/679 (51.84%), Query Frame = 0

Query: 1   MPFFYKSASNSRIQQK--VPDSEFSVGLPEPLVQRRLTRQRKLRHLTDQDVGLQHRRSFP 60
           MP++ KS    +   K  + D+ F+  L      R   R RK R   D+ V    R +  
Sbjct: 1   MPWWSKSKDEKKKTNKESIIDA-FNRKLGFASEDRSSGRSRKSRRRRDEIV--SERGAIS 60

Query: 61  DSPDLSAKPRSPLGG----SERWSSEALPQPLPLPEYFPLRNPESGSWSSGQGRVGSPIE 120
             P  S  P + +      +ER  +  LP+P+  P      +  +GS   G      P  
Sbjct: 61  RLPSRSPSPSTRVSRCQSFAERSPAVPLPRPIVRPHVTSTDSGMNGSQRPGLDANLKPSW 120

Query: 121 APVRKSSDHSTTNANRSLGNNQRRVAIDFA-LEVANGNTGG----------------NGA 180
            P+ K   H  T+   + G        DFA   V++G++ G                NG 
Sbjct: 121 LPLPK--PHGATSIPDNTGAEP-----DFATASVSSGSSVGDIPSDSLLSPLASDCENGN 180

Query: 181 TTFTIPQSLSVNSGKSNSNKEFVFVCLDQPPNNK-----GRGSPTHPSVERGNFNLTPKT 240
            T   P ++S      +SNK    +    P  N+      R  P    V+  N  +  + 
Sbjct: 181 RT---PVNISSRDQSMHSNKNSAEMFKPVPNKNRILSASPRRRPLGTHVK--NLQIPQRD 240

Query: 241 SSSKSAPTSILPSPAVSPRRSYTGD------LFVS------GLTRHEHQESP------SN 300
               SAP S+L SP+ SP RS+  D      L +S       L       SP       N
Sbjct: 241 LVLCSAPDSLLSSPSRSPMRSFIPDQVSNHGLLISKPYSDVSLLGSGQCSSPGSGYNSGN 300

Query: 301 NS------PKVPTLKTAHSSQPSPLHSPTTRSSASNSRSPNDMPFPLHHKL--QKDSSID 360
           NS       ++   ++  S + SP+ SP   S   +SR  +    PLH +       S  
Sbjct: 301 NSIGGDMATQLFWPQSRCSPECSPVPSPRMTSPGPSSRIQSGAVTPLHPRAGGSTTGSPT 360

Query: 361 RSENHGHVNAHPLPLPPPLIA-CSQSSPQSHSSNVHHVIEKPFIS----SMKCQWQKGKL 420
           R  +     +H LPLPP LI+     SP   ++    V   P  +    S   +W+KG+L
Sbjct: 361 RRLDDNRQQSHRLPLPPLLISNTCPFSPTYSAATSPSVPRSPARAEATVSPGSRWKKGRL 420

Query: 421 IGRGTFGSVYLATNRETGALCAMKEVDLIPDDPKSAESIKQLEQEIKVLSQLKHPNIVQY 480
           +G G+FG VYL  N E+G +CAMKEV L  DDPKS ES +QL QEI VLS+L+H NIVQY
Sbjct: 421 LGMGSFGHVYLGFNSESGEMCAMKEVTLCSDDPKSRESAQQLGQEISVLSRLRHQNIVQY 480

Query: 481 YGSEIIDDHFYIYLEYVFPGSINKYVREHCGAITESIVRNFTRHILSGLAYLHSTKTIHR 540
           YGSE +DD  YIYLEYV  GSI K ++E+ G   E+ +RN+T+ ILSGLAYLH+  T+HR
Sbjct: 481 YGSETVDDKLYIYLEYVSGGSIYKLLQEY-GQFGENAIRNYTQQILSGLAYLHAKNTVHR 540

Query: 541 DIKGANLLVDSSGVVKLADFGMAKHLTGQY-DLSLKGSPYWMAPEVIKAAMLKDINPDLA 600
           DIKGAN+LVD  G VK+ADFGMAKH+T Q   LS KGSPYWMAPEVIK +   +      
Sbjct: 541 DIKGANILVDPHGRVKVADFGMAKHITAQSGPLSFKGSPYWMAPEVIKNSNGSN------ 600

Query: 601 LAVDIWSLGCTIIEMLNGKPPWSEYEGHQAMFKVLN--KTPPIPEKLSPEGKDFLQCCFQ 618
           LAVDIWSLGCT++EM   KPPWS+YEG  AMFK+ N  + P IP+ LS EGKDF++ C Q
Sbjct: 601 LAVDIWSLGCTVLEMATTKPPWSQYEGVPAMFKIGNSKELPDIPDHLSEEGKDFVRKCLQ 657

BLAST of Lag0004325 vs. ExPASy Swiss-Prot
Match: F4HRJ4 (Mitogen-activated protein kinase kinase kinase 3 OS=Arabidopsis thaliana OX=3702 GN=MAPKKK3 PE=1 SV=1)

HSP 1 Score: 320.1 bits (819), Expect = 6.3e-86
Identity = 171/333 (51.35%), Postives = 219/333 (65.77%), Query Frame = 0

Query: 294 PLHHKLQKDSSIDRS-----ENHGHVNAHPLPLPPPLIACSQSSPQS-HSSNVHHVIEKP 353
           P H +L    S++ S     +       HPLP PP     S +SP + H S +    E  
Sbjct: 152 PRHQRLSGVVSLESSTGRNDDGRSSSECHPLPRPP----TSPTSPSAVHGSRIGGGYETS 211

Query: 354 FISSMKCQWQKGKLIGRGTFGSVYLATNRETGALCAMKEVDLIPDDPKSAESIKQLEQEI 413
              S    W+KGK +G GTFG VYL  N E G +CA+KEV +I DD  S E +KQL QEI
Sbjct: 212 --PSGFSTWKKGKFLGSGTFGQVYLGFNSEKGKMCAIKEVKVISDDQTSKECLKQLNQEI 271

Query: 414 KVLSQLKHPNIVQYYGSEIIDDHFYIYLEYVFPGSINKYVREHCGAITESIVRNFTRHIL 473
            +L+QL HPNIVQYYGSE+ ++   +YLEYV  GSI+K ++++ G+ TE +++N+TR IL
Sbjct: 272 NLLNQLCHPNIVQYYGSELSEETLSVYLEYVSGGSIHKLLKDY-GSFTEPVIQNYTRQIL 331

Query: 474 SGLAYLHSTKTIHRDIKGANLLVDSSGVVKLADFGMAKHLTG-QYDLSLKGSPYWMAPEV 533
           +GLAYLH   T+HRDIKGAN+LVD +G +KLADFGMAKH+T     LS KGSPYWMAPEV
Sbjct: 332 AGLAYLHGRNTVHRDIKGANILVDPNGEIKLADFGMAKHVTAFSTMLSFKGSPYWMAPEV 391

Query: 534 IKAAMLKDINPDLALAVDIWSLGCTIIEMLNGKPPWSEYEGHQAMFKVLNK--TPPIPEK 593
           + +            AVDIWSLGCTI+EM   KPPWS++EG  A+FK+ N   TP IP+ 
Sbjct: 392 VMS------QNGYTHAVDIWSLGCTILEMATSKPPWSQFEGVAAIFKIGNSKDTPEIPDH 451

Query: 594 LSPEGKDFLQCCFQRNPADRPTAMVLLDHPFLR 618
           LS + K+F++ C QRNP  RPTA  LL+HPFLR
Sbjct: 452 LSNDAKNFIRLCLQRNPTVRPTASQLLEHPFLR 471

BLAST of Lag0004325 vs. ExPASy Swiss-Prot
Match: Q54R82 (Mitogen-activated protein kinase kinase kinase A OS=Dictyostelium discoideum OX=44689 GN=mkkA PE=1 SV=2)

HSP 1 Score: 242.3 bits (617), Expect = 1.7e-62
Identity = 125/292 (42.81%), Postives = 186/292 (63.70%), Query Frame = 0

Query: 349 ISSMKCQWQKGKLIGRGTFGSVYLATNRETGALCAMKEVDL--IPDDPKSAESIKQLEQE 408
           IS+   +WQKG+++GRG +GSVYL  N++TG L A+K++++  I  DPK    I    +E
Sbjct: 163 ISNHNIKWQKGQILGRGGYGSVYLGLNKDTGELFAVKQLEIVDINSDPKLKNMILSFSKE 222

Query: 409 IKVLSQLKHPNIVQYYGSEIIDDHFYIYLEYVFPGSINKYVREHCGAITESIVRNFTRHI 468
           I+V+  L+H NIV+Y G+ +      ++LEY+  GSI+  + +  GA +E++++ +T+ I
Sbjct: 223 IEVMRSLRHDNIVRYLGTSLDQSFLSVFLEYIPGGSISSLLGKF-GAFSENVIKVYTKQI 282

Query: 469 LSGLAYLHSTKTIHRDIKGANLLVDSSGVVKLADFGMAKHLTG--QYDLSLKGSPYWMAP 528
           L GL++LH+   IHRDIKGAN+L+D+ G+VKL+DFG +K  +G      S++G+PYWMAP
Sbjct: 283 LQGLSFLHANSIIHRDIKGANILIDTKGIVKLSDFGCSKSFSGIVSQFKSMQGTPYWMAP 342

Query: 529 EVIKAAMLKDINPDLALAVDIWSLGCTIIEMLNGKPPWSEY-EGHQAMFKVL--NKTPPI 588
           EVIK             + DIWSLGC I+EM   +PPWS   E    M+ +   N  P I
Sbjct: 343 EVIK-------QTGHGRSSDIWSLGCVIVEMATAQPPWSNITELAAVMYHIASSNSIPNI 402

Query: 589 PEKLSPEGKDFLQCCFQRNPADRPTAMVLLDHPFLRFSNDSNVSIPTSAFST 634
           P  +S E  DFL  CF+R+P +RP A  LL HPF+  + D N+ +PT + +T
Sbjct: 403 PSHMSQEAFDFLNLCFKRDPKERPDANQLLKHPFI-MNLDDNIQLPTISPTT 445

BLAST of Lag0004325 vs. ExPASy Swiss-Prot
Match: Q9FZ36 (Mitogen-activated protein kinase kinase kinase 2 OS=Arabidopsis thaliana OX=3702 GN=ANP2 PE=2 SV=1)

HSP 1 Score: 238.0 bits (606), Expect = 3.2e-61
Identity = 141/345 (40.87%), Postives = 199/345 (57.68%), Query Frame = 0

Query: 303 SSIDRSENHGHVNAHPLPLPPPLIACSQSS-----PQSHSSNVHHVIEKPFISSMKCQWQ 362
           S+ D      H    P  L   + +C + S      QS  +N    I+ P       +W+
Sbjct: 16  STTDDENQENHPPPFPSLLADKITSCIRKSMVFAKSQSPPNNSTVQIKPPI------RWR 75

Query: 363 KGKLIGRGTFGSVYLATNRETGALCAMKEVDLIPDDPKSAES----IKQLEQEIKVLSQL 422
           KG+LIGRG FG+VY+  N ++G L A+K+V LI  +  S E     I++LE+E+K+L  L
Sbjct: 76  KGQLIGRGAFGTVYMGMNLDSGELLAVKQV-LITSNCASKEKTQAHIQELEEEVKLLKNL 135

Query: 423 KHPNIVQYYGSEIIDDHFYIYLEYVFPGSINKYVREHCGAITESIVRNFTRHILSGLAYL 482
            HPNIV+Y G+   D+   I LE+V  GSI+  + E  GA  ES+VR +T  +L GL YL
Sbjct: 136 SHPNIVRYLGTVREDETLNILLEFVPGGSISSLL-EKFGAFPESVVRTYTNQLLLGLEYL 195

Query: 483 HSTKTIHRDIKGANLLVDSSGVVKLADFGMAKHLTGQYDL----SLKGSPYWMAPEVIKA 542
           H+   +HRDIKGAN+LVD+ G +KLADFG +K +     +    S+KG+PYWMAPEVI  
Sbjct: 196 HNHAIMHRDIKGANILVDNQGCIKLADFGASKQVAELATISGAKSMKGTPYWMAPEVI-- 255

Query: 543 AMLKDINPDLALAVDIWSLGCTIIEMLNGKPPWS-EYEGHQAMFKV--LNKTPPIPEKLS 602
                +    + + DIWS+GCT+IEM+ GK PWS +Y+   A+F +      PPIP+ +S
Sbjct: 256 -----LQTGHSFSADIWSVGCTVIEMVTGKAPWSQQYKEIAAIFHIGTTKSHPPIPDNIS 315

Query: 603 PEGKDFLQCCFQRNPADRPTAMVLLDHPFLRFSNDSNVSIPTSAF 632
            +  DFL  C Q+ P  RPTA  LL HPF+      + S   ++F
Sbjct: 316 SDANDFLLKCLQQEPNLRPTASELLKHPFVTGKQKESASKDLTSF 345

BLAST of Lag0004325 vs. ExPASy TrEMBL
Match: A0A1S3BBK6 (mitogen-activated protein kinase kinase kinase YODA-like isoform X2 OS=Cucumis melo OX=3656 GN=LOC103487921 PE=4 SV=1)

HSP 1 Score: 1198.0 bits (3098), Expect = 0.0e+00
Identity = 599/709 (84.49%), Postives = 635/709 (89.56%), Query Frame = 0

Query: 1   MPFFYKSASNSRIQQKVPD------SEFSVGLPEPLVQRRLTRQRKLRHLTDQDVGLQHR 60
           MPFF+KSASNSR+QQKVPD      S+FSVGLPEP VQRRLTRQRKLRHLTD+DVG +  
Sbjct: 1   MPFFHKSASNSRLQQKVPDSDFSDFSDFSVGLPEPFVQRRLTRQRKLRHLTDRDVGFEQP 60

Query: 61  RSFPDSPDLSAKPRSPLGGSERWSSEALPQPLPLPEYFPLRNPESGSWSSGQGRVGSPIE 120
           RS PDSPD+SAK +SPLGGSERWSS   PQPLPLPE FP+R+PESG W+S QGRVGSP E
Sbjct: 61  RSLPDSPDMSAKSKSPLGGSERWSSSPSPQPLPLPELFPVRSPESGCWNSVQGRVGSPDE 120

Query: 121 APVRKSSDHSTTNANRSLGNNQRRVAIDFALEVANGNTGGNGATTFTIPQSLSVNSGKSN 180
            PVRK SDH+TTN +RS G+N+RRVA D  LEV NGN+ G  ATTFT PQS SVNSGK N
Sbjct: 121 TPVRKCSDHATTNVSRSFGHNERRVATDLTLEVVNGNSRGTAATTFTSPQSFSVNSGKLN 180

Query: 181 SNKEFVFVCLDQPPNNKGRGSPTHPSVERGNFNLTPKTSSSKSAPTSILPSPAVSPRRSY 240
            NKEF+F CLDQ PNN GRGSPTHPS ERGN+NLT K SSSKSAPTSILPSP VSPR SY
Sbjct: 181 LNKEFLFGCLDQTPNNNGRGSPTHPSTERGNYNLTSKNSSSKSAPTSILPSPVVSPRTSY 240

Query: 241 TGDLFVSGLTRHEHQESPSNNSPKVPTLKTAHSSQPSPLHSPTTRSSASNSRSPNDMPFP 300
            G+  VSGLT HE+QESPSNNSPKVP LKTA SSQP PLHSPT RSS SNSR PNDM FP
Sbjct: 241 NGNHVVSGLTHHEYQESPSNNSPKVPPLKTALSSQPFPLHSPTARSSISNSRPPNDMTFP 300

Query: 301 LHHKLQKDSSIDRSENHGHVNAHPLPLPPPLIACSQSSPQSHSSNVHHVIEKPFISSMKC 360
           LH KLQKD+SIDRSE+H HVNAHPLPLPPPL+A SQ+SPQS  SNVHHVIEKP+ISSMK 
Sbjct: 301 LHPKLQKDNSIDRSESHAHVNAHPLPLPPPLVASSQASPQSLPSNVHHVIEKPYISSMKG 360

Query: 361 QWQKGKLIGRGTFGSVYLATNRETGALCAMKEVDLIPDDPKSAESIKQLEQEIKVLSQLK 420
           QWQKGKLIGRGTFGSVYLATNRETGALCAMKEVDLIPDDPKSAE IKQLEQEI+VLS LK
Sbjct: 361 QWQKGKLIGRGTFGSVYLATNRETGALCAMKEVDLIPDDPKSAECIKQLEQEIEVLSHLK 420

Query: 421 HPNIVQYYGSEIIDDHFYIYLEYVFPGSINKYVREHCGAITESIVRNFTRHILSGLAYLH 480
           HPNIVQYYGSEII D+FYIYLEYV+PGSINKYVREHCGAITESIVRNFTRHILSGLAYLH
Sbjct: 421 HPNIVQYYGSEIIGDYFYIYLEYVYPGSINKYVREHCGAITESIVRNFTRHILSGLAYLH 480

Query: 481 STKTIHRDIKGANLLVDSSGVVKLADFGMAKHLTGQYDLSLKGSPYWMAPEVIKAAMLKD 540
           STKTIHRDIKGANLLVDSSG VKLADFGMAKHLTGQYDLSLKGSPYWMAPEVIKAAMLKD
Sbjct: 481 STKTIHRDIKGANLLVDSSGAVKLADFGMAKHLTGQYDLSLKGSPYWMAPEVIKAAMLKD 540

Query: 541 INPDLALAVDIWSLGCTIIEMLNGKPPWSEYEGHQAMFKVLNKTPPIPEKLSPEGKDFLQ 600
            NPDLALAVDIWSLGCTIIEMLNGKPPW E+EGHQAMFKVLNKTPPIPEKLSPEGKDFLQ
Sbjct: 541 ANPDLALAVDIWSLGCTIIEMLNGKPPWCEFEGHQAMFKVLNKTPPIPEKLSPEGKDFLQ 600

Query: 601 CCFQRNPADRPTAMVLLDHPFLRFSNDSNVSIPTSAFSTMNLLEKLLSPKDHLHPKGDQG 660
           CCFQRNPADRP+A+VLLDHPFLR S+DSNVSIPTSAFSTM LLEKLL+PKD L+PKGDQ 
Sbjct: 601 CCFQRNPADRPSAVVLLDHPFLRSSSDSNVSIPTSAFSTMKLLEKLLNPKDPLNPKGDQA 660

Query: 661 QNSPGTSAANDYLPCRSPSTNIPSNIPASAVNNHHFALSRTHTREVPHL 704
           QNSPGTS +NDYL CRSPSTNI SNIPASAV NHHF++SRTH REVPHL
Sbjct: 661 QNSPGTSTSNDYLTCRSPSTNISSNIPASAVVNHHFSISRTHKREVPHL 709

BLAST of Lag0004325 vs. ExPASy TrEMBL
Match: A0A1S3BAV6 (mitogen-activated protein kinase kinase kinase YODA-like isoform X1 OS=Cucumis melo OX=3656 GN=LOC103487921 PE=4 SV=1)

HSP 1 Score: 1190.6 bits (3079), Expect = 0.0e+00
Identity = 599/717 (83.54%), Postives = 635/717 (88.56%), Query Frame = 0

Query: 1   MPFFYKSASNSRIQQKVPD------SEFSVGLPEPLVQRRLTRQRKLRHLTDQDVGLQHR 60
           MPFF+KSASNSR+QQKVPD      S+FSVGLPEP VQRRLTRQRKLRHLTD+DVG +  
Sbjct: 1   MPFFHKSASNSRLQQKVPDSDFSDFSDFSVGLPEPFVQRRLTRQRKLRHLTDRDVGFEQP 60

Query: 61  RSFPDSPDLSAKPRSPLGGSERWSSEALPQPLPLPEYFPLRNPESGSWSSGQGRVGSPIE 120
           RS PDSPD+SAK +SPLGGSERWSS   PQPLPLPE FP+R+PESG W+S QGRVGSP E
Sbjct: 61  RSLPDSPDMSAKSKSPLGGSERWSSSPSPQPLPLPELFPVRSPESGCWNSVQGRVGSPDE 120

Query: 121 AP--------VRKSSDHSTTNANRSLGNNQRRVAIDFALEVANGNTGGNGATTFTIPQSL 180
            P        VRK SDH+TTN +RS G+N+RRVA D  LEV NGN+ G  ATTFT PQS 
Sbjct: 121 TPVRNSAIKTVRKCSDHATTNVSRSFGHNERRVATDLTLEVVNGNSRGTAATTFTSPQSF 180

Query: 181 SVNSGKSNSNKEFVFVCLDQPPNNKGRGSPTHPSVERGNFNLTPKTSSSKSAPTSILPSP 240
           SVNSGK N NKEF+F CLDQ PNN GRGSPTHPS ERGN+NLT K SSSKSAPTSILPSP
Sbjct: 181 SVNSGKLNLNKEFLFGCLDQTPNNNGRGSPTHPSTERGNYNLTSKNSSSKSAPTSILPSP 240

Query: 241 AVSPRRSYTGDLFVSGLTRHEHQESPSNNSPKVPTLKTAHSSQPSPLHSPTTRSSASNSR 300
            VSPR SY G+  VSGLT HE+QESPSNNSPKVP LKTA SSQP PLHSPT RSS SNSR
Sbjct: 241 VVSPRTSYNGNHVVSGLTHHEYQESPSNNSPKVPPLKTALSSQPFPLHSPTARSSISNSR 300

Query: 301 SPNDMPFPLHHKLQKDSSIDRSENHGHVNAHPLPLPPPLIACSQSSPQSHSSNVHHVIEK 360
            PNDM FPLH KLQKD+SIDRSE+H HVNAHPLPLPPPL+A SQ+SPQS  SNVHHVIEK
Sbjct: 301 PPNDMTFPLHPKLQKDNSIDRSESHAHVNAHPLPLPPPLVASSQASPQSLPSNVHHVIEK 360

Query: 361 PFISSMKCQWQKGKLIGRGTFGSVYLATNRETGALCAMKEVDLIPDDPKSAESIKQLEQE 420
           P+ISSMK QWQKGKLIGRGTFGSVYLATNRETGALCAMKEVDLIPDDPKSAE IKQLEQE
Sbjct: 361 PYISSMKGQWQKGKLIGRGTFGSVYLATNRETGALCAMKEVDLIPDDPKSAECIKQLEQE 420

Query: 421 IKVLSQLKHPNIVQYYGSEIIDDHFYIYLEYVFPGSINKYVREHCGAITESIVRNFTRHI 480
           I+VLS LKHPNIVQYYGSEII D+FYIYLEYV+PGSINKYVREHCGAITESIVRNFTRHI
Sbjct: 421 IEVLSHLKHPNIVQYYGSEIIGDYFYIYLEYVYPGSINKYVREHCGAITESIVRNFTRHI 480

Query: 481 LSGLAYLHSTKTIHRDIKGANLLVDSSGVVKLADFGMAKHLTGQYDLSLKGSPYWMAPEV 540
           LSGLAYLHSTKTIHRDIKGANLLVDSSG VKLADFGMAKHLTGQYDLSLKGSPYWMAPEV
Sbjct: 481 LSGLAYLHSTKTIHRDIKGANLLVDSSGAVKLADFGMAKHLTGQYDLSLKGSPYWMAPEV 540

Query: 541 IKAAMLKDINPDLALAVDIWSLGCTIIEMLNGKPPWSEYEGHQAMFKVLNKTPPIPEKLS 600
           IKAAMLKD NPDLALAVDIWSLGCTIIEMLNGKPPW E+EGHQAMFKVLNKTPPIPEKLS
Sbjct: 541 IKAAMLKDANPDLALAVDIWSLGCTIIEMLNGKPPWCEFEGHQAMFKVLNKTPPIPEKLS 600

Query: 601 PEGKDFLQCCFQRNPADRPTAMVLLDHPFLRFSNDSNVSIPTSAFSTMNLLEKLLSPKDH 660
           PEGKDFLQCCFQRNPADRP+A+VLLDHPFLR S+DSNVSIPTSAFSTM LLEKLL+PKD 
Sbjct: 601 PEGKDFLQCCFQRNPADRPSAVVLLDHPFLRSSSDSNVSIPTSAFSTMKLLEKLLNPKDP 660

Query: 661 LHPKGDQGQNSPGTSAANDYLPCRSPSTNIPSNIPASAVNNHHFALSRTHTREVPHL 704
           L+PKGDQ QNSPGTS +NDYL CRSPSTNI SNIPASAV NHHF++SRTH REVPHL
Sbjct: 661 LNPKGDQAQNSPGTSTSNDYLTCRSPSTNISSNIPASAVVNHHFSISRTHKREVPHL 717

BLAST of Lag0004325 vs. ExPASy TrEMBL
Match: A0A0A0LLB6 (Protein kinase domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_2G360650 PE=4 SV=1)

HSP 1 Score: 1182.2 bits (3057), Expect = 0.0e+00
Identity = 597/706 (84.56%), Postives = 630/706 (89.24%), Query Frame = 0

Query: 1   MPFFYKSASNSRIQQKVPDS---EFSVGLPEPLVQRRLTRQRKLRHLTDQDVGLQHRRSF 60
           MP F KSASNSR+QQKVPDS   +FSVGLPEP +QRRLTRQRKLRHLTDQDVG +H RS 
Sbjct: 1   MPSFNKSASNSRLQQKVPDSDFFDFSVGLPEPFIQRRLTRQRKLRHLTDQDVGFEHPRSL 60

Query: 61  PDSPDLSAKPRSPLGGSERWSSEALPQPLPLPEYFPLRNPESGSWSSGQGRVGSPIEAPV 120
           PDSPD+SAKP+SPLGGSERWSS   PQPLPLPE FP+R+PE GS + GQGRVGSP+E  V
Sbjct: 61  PDSPDISAKPKSPLGGSERWSSSPSPQPLPLPELFPVRSPEFGS-NYGQGRVGSPVETSV 120

Query: 121 RKSSDHSTTNANRSLGNNQRRVAIDFALEVANGNTGGNGATTFTIPQSLSVNSGKSNSNK 180
           RK SDH+TTN +RS G+NQRRVA D  LEV +GN+   GAT FT PQSLS NSGK N NK
Sbjct: 121 RKCSDHATTNVSRSFGHNQRRVATDLTLEVVDGNS-RTGATAFTSPQSLSANSGKLNPNK 180

Query: 181 EFVFVCLDQPPNNKGRGSPTHPSVERGNFNLTPKTSSSKSAPTSILPSPAVSPRRSYTGD 240
           EF+F C DQ PNNKGRGSPTHPS ERGN+NLTPK SSSKSAPTSILPSP VSPR+SY G+
Sbjct: 181 EFLFGCSDQTPNNKGRGSPTHPSAERGNYNLTPKKSSSKSAPTSILPSPVVSPRKSYNGN 240

Query: 241 LFVSGLTRHEHQESPSNNSPKVPTLKTAHSSQPSPLHSPTTRSSASNSRSPNDMPFPLHH 300
            FV GL+ HEHQESPSNNSPKVP LKTA SSQP PLHSPT RSS SNSRS NDM FPLH 
Sbjct: 241 HFVPGLSHHEHQESPSNNSPKVPPLKTALSSQPFPLHSPTARSSISNSRSHNDMTFPLHT 300

Query: 301 KLQKDSSIDRSENHGHVNAHPLPLPPPLIACSQSSPQSHSSNVHHVIEKPFISSMKCQWQ 360
           KLQKD+SIDRSE+H HVNAHPLPLPPPL+A SQ+S QS  SNVHHVIEKPFISSMK QWQ
Sbjct: 301 KLQKDNSIDRSESHAHVNAHPLPLPPPLVASSQASAQSLPSNVHHVIEKPFISSMKGQWQ 360

Query: 361 KGKLIGRGTFGSVYLATNRETGALCAMKEVDLIPDDPKSAESIKQLEQEIKVLSQLKHPN 420
           KGKLIGRGTFGSVYLATNRETGALCAMKEVDLIPDDPKSAE IKQLEQEI+VLS LKHPN
Sbjct: 361 KGKLIGRGTFGSVYLATNRETGALCAMKEVDLIPDDPKSAECIKQLEQEIEVLSHLKHPN 420

Query: 421 IVQYYGSEIIDDHFYIYLEYVFPGSINKYVREHCGAITESIVRNFTRHILSGLAYLHSTK 480
           IVQYYGSEII D FYIYLEYV+PGSINKYVRE CGAITESIVRNFTRHILSGLAYLHSTK
Sbjct: 421 IVQYYGSEIIGDCFYIYLEYVYPGSINKYVRERCGAITESIVRNFTRHILSGLAYLHSTK 480

Query: 481 TIHRDIKGANLLVDSSGVVKLADFGMAKHLTGQYDLSLKGSPYWMAPEVIKAAMLKDINP 540
           TIHRDIKGANLLVDSSGVVKLADFGMAKHLTGQYDLSLKGSPYWMAPEVIKAAMLKD NP
Sbjct: 481 TIHRDIKGANLLVDSSGVVKLADFGMAKHLTGQYDLSLKGSPYWMAPEVIKAAMLKDANP 540

Query: 541 DLALAVDIWSLGCTIIEMLNGKPPWSEYEGHQAMFKVLNKTPPIPEKLSPEGKDFLQCCF 600
           DLALAVDIWSLGCTIIEMLNGKPPW E+EGHQ MFKVLNKTPPIPEKLSPEGKDFLQCCF
Sbjct: 541 DLALAVDIWSLGCTIIEMLNGKPPWCEFEGHQVMFKVLNKTPPIPEKLSPEGKDFLQCCF 600

Query: 601 QRNPADRPTAMVLLDHPFLRFSNDSNVSIPTSAFSTMNLLEKLLSPKDHLHPKGDQGQNS 660
           QRNPADRPTAMVLLDHPFLR S+DSN SIPTSAFSTMNLLEKLLSPKD L+PKGDQ QNS
Sbjct: 601 QRNPADRPTAMVLLDHPFLRSSSDSNASIPTSAFSTMNLLEKLLSPKDPLNPKGDQAQNS 660

Query: 661 PGTSAANDYLPCRSPSTNIPSNIPASAVNNHHFALSRTHTREVPHL 704
            GT  +NDYL CRSPSTNI SN+PASAV NHHF++SRTH REVPHL
Sbjct: 661 SGTLTSNDYLSCRSPSTNISSNVPASAVANHHFSISRTHKREVPHL 704

BLAST of Lag0004325 vs. ExPASy TrEMBL
Match: A0A6J1HD45 (mitogen-activated protein kinase kinase kinase 5-like OS=Cucurbita moschata OX=3662 GN=LOC111462409 PE=4 SV=1)

HSP 1 Score: 1182.2 bits (3057), Expect = 0.0e+00
Identity = 598/704 (84.94%), Postives = 627/704 (89.06%), Query Frame = 0

Query: 1   MPFFYKSASNSRIQQKVPDSEFSVGLPEPLVQRRLTRQRKLRHLTDQDVGLQHRRSFPDS 60
           MPFF+KSASNSR+QQK  DS FSV   E LVQRRLTRQRKLRHLTD+++G++  RSFPDS
Sbjct: 1   MPFFHKSASNSRLQQKAADSNFSVVQTESLVQRRLTRQRKLRHLTDREIGVELPRSFPDS 60

Query: 61  PDLSAKPRSPLGGSERWSSEALPQPLPLPEYFPLRNPESGSWSSGQGRVGSPIEAPVRKS 120
           PDLSAKPRSPLGGSERWSS ALPQPLPLPE FP+RNPES SW SGQGRV SPIE PVRKS
Sbjct: 61  PDLSAKPRSPLGGSERWSSPALPQPLPLPELFPVRNPESDSWFSGQGRVVSPIETPVRKS 120

Query: 121 SDHSTTNANRSLGNNQRRVAIDFALEVANGNTGGNGATTFTIPQSLSVNSGKSNSNKEFV 180
           SD +TTN N  LGNNQRRVA D  LEV NGNTGG GATTFT P S SVNSGKS S KEFV
Sbjct: 121 SDRATTNVNGGLGNNQRRVATDCKLEVVNGNTGGTGATTFTSPHSPSVNSGKSLSIKEFV 180

Query: 181 FVCLDQPPNNKGRGSPTHPSVERGNFNLTPKTSSSKSAPTSILPSPAVSPRRSYTGDLFV 240
           F CLDQPPN+K RGSPTHPS++RGN++LTPKTSSSKSAPTSILPSP  SPRRSYTGDLFV
Sbjct: 181 FGCLDQPPNSKVRGSPTHPSIQRGNYSLTPKTSSSKSAPTSILPSPTASPRRSYTGDLFV 240

Query: 241 SGLTRHEHQESPSNNSPKVPTLKTAHSSQPSPLHSPTTRSSASNSRSPNDMPFPLHHKLQ 300
           SGL RHE+QESP NNSPK P LKT H+SQPSPLHSP+ RS  S  +SPND+ FP H KLQ
Sbjct: 241 SGLPRHEYQESPFNNSPKFPPLKTPHTSQPSPLHSPSARSFVSGPKSPNDVKFPTHSKLQ 300

Query: 301 KD-SSIDRSENHGHVNAHPLPLPPPLIACSQSSPQSHSSNVHHVIEKPFISSMKCQWQKG 360
           KD SS DRSEN GHVNAHPLPLPPPL   SQSSP S SS+VH V EKP+ISS K +WQKG
Sbjct: 301 KDCSSTDRSENLGHVNAHPLPLPPPLAHSSQSSPLSLSSSVHRVNEKPYISSTKSRWQKG 360

Query: 361 KLIGRGTFGSVYLATNRETGALCAMKEVDLIPDDPKSAESIKQLEQEIKVLSQLKHPNIV 420
           KLIGRGTFGSVYLATN ETGALCAMKEVDLIPDDPK AESIKQLEQEIKVLSQLKHPNIV
Sbjct: 361 KLIGRGTFGSVYLATNSETGALCAMKEVDLIPDDPKCAESIKQLEQEIKVLSQLKHPNIV 420

Query: 421 QYYGSEIIDDHFYIYLEYVFPGSINKYVREHCGAITESIVRNFTRHILSGLAYLHSTKTI 480
           QYYGSEIIDDHFYIYLEYV+PGSINKYVRE CGAITESIVRNFTRHILSGLAYLHSTKTI
Sbjct: 421 QYYGSEIIDDHFYIYLEYVYPGSINKYVRERCGAITESIVRNFTRHILSGLAYLHSTKTI 480

Query: 481 HRDIKGANLLVDSSGVVKLADFGMAKHLTGQYDLSLKGSPYWMAPEVIKAAMLKDINPDL 540
           HRDIKGANLLVDSSGVVKLADFGMAKHLTGQYDLSLKGSPYWMAPEV+KAAMLKD NP+L
Sbjct: 481 HRDIKGANLLVDSSGVVKLADFGMAKHLTGQYDLSLKGSPYWMAPEVMKAAMLKDANPNL 540

Query: 541 ALAVDIWSLGCTIIEMLNGKPPWSEYEGHQAMFKVLNKTPPIPEKLSPEGKDFLQCCFQR 600
           ALAVDIWSLGCTIIEML+GKPPWSE+EGHQAMFKVLNKTPPIPEKLSPEGKDFLQCCFQR
Sbjct: 541 ALAVDIWSLGCTIIEMLDGKPPWSEFEGHQAMFKVLNKTPPIPEKLSPEGKDFLQCCFQR 600

Query: 601 NPADRPTAMVLLDHPFLRFSNDSNVSIPTSAFSTMNLLEKLLSPKDHLHPKGDQGQNSPG 660
           NPADRPTAM LLDHPFLR SNDSNVS PTSAFSTMN +EKLLSPKDH  PKGDQ QNS G
Sbjct: 601 NPADRPTAMALLDHPFLRSSNDSNVSTPTSAFSTMNRMEKLLSPKDHRSPKGDQAQNSSG 660

Query: 661 TSAANDYLPCRSPSTNIPSNIPASAVNNHHFALSRTHTREVPHL 704
           T A N YL CRSPSTNI SNIPASAVNNH FA+SRTH REVPHL
Sbjct: 661 TLAENGYLSCRSPSTNISSNIPASAVNNHPFAISRTHKREVPHL 704

BLAST of Lag0004325 vs. ExPASy TrEMBL
Match: A0A6J1K5B8 (mitogen-activated protein kinase kinase kinase 5-like OS=Cucurbita maxima OX=3661 GN=LOC111492436 PE=4 SV=1)

HSP 1 Score: 1176.4 bits (3042), Expect = 0.0e+00
Identity = 590/703 (83.93%), Postives = 624/703 (88.76%), Query Frame = 0

Query: 1   MPFFYKSASNSRIQQKVPDSEFSVGLPEPLVQRRLTRQRKLRHLTDQDVGLQHRRSFPDS 60
           MPFF+KSASNSR+QQK  DS+FSV   E LVQRRLTRQRKLRHLTD+++G++  RSFPDS
Sbjct: 1   MPFFHKSASNSRLQQKAADSDFSVVQTESLVQRRLTRQRKLRHLTDREIGVELPRSFPDS 60

Query: 61  PDLSAKPRSPLGGSERWSSEALPQPLPLPEYFPLRNPESGSWSSGQGRVGSPIEAPVRKS 120
           PDLSAKPRSPLGGSERWSS  LPQPLPLPE FP+RNPES SW + QGRV SPIE PVRKS
Sbjct: 61  PDLSAKPRSPLGGSERWSSSPLPQPLPLPELFPVRNPESDSWFASQGRVVSPIETPVRKS 120

Query: 121 SDHSTTNANRSLGNNQRRVAIDFALEVANGNTGGNGATTFTIPQSLSVNSGKSNSNKEFV 180
           SD +TTN N  LGNNQRRVA D  LEV NGNTGG GA TFT P S SVNSGKS S KEFV
Sbjct: 121 SDRATTNVNGGLGNNQRRVATDCKLEVVNGNTGGTGANTFTSPHSPSVNSGKSISIKEFV 180

Query: 181 FVCLDQPPNNKGRGSPTHPSVERGNFNLTPKTSSSKSAPTSILPSPAVSPRRSYTGDLFV 240
           F CLDQPPN+K RGSPTHPS+ RGN++LTPKT SSKSAPTSILPSP  SPRRSYTGDLFV
Sbjct: 181 FSCLDQPPNSKVRGSPTHPSIRRGNYSLTPKTLSSKSAPTSILPSPTASPRRSYTGDLFV 240

Query: 241 SGLTRHEHQESPSNNSPKVPTLKTAHSSQPSPLHSPTTRSSASNSRSPNDMPFPLHHKLQ 300
           SGL RH++QESP NNSP+VP LKT H+SQPSPLHSP+ RS  SN +SPND+ FP H KLQ
Sbjct: 241 SGLPRHDYQESPFNNSPRVPPLKTPHTSQPSPLHSPSARSFVSNPKSPNDVKFPTHSKLQ 300

Query: 301 KDSSIDRSENHGHVNAHPLPLPPPLIACSQSSPQSHSSNVHHVIEKPFISSMKCQWQKGK 360
           KDSS D SEN GHVNAHPLPLPPPL   SQSSPQS SS+VHHV EKP+ISS K +WQKGK
Sbjct: 301 KDSSTDWSENLGHVNAHPLPLPPPLAHSSQSSPQSLSSSVHHVNEKPYISSTKSRWQKGK 360

Query: 361 LIGRGTFGSVYLATNRETGALCAMKEVDLIPDDPKSAESIKQLEQEIKVLSQLKHPNIVQ 420
           LIGRGTFGSVYLATN ETGALCAMKEVDLIPDDPK AESIKQLEQEIKVLSQLKHPNIVQ
Sbjct: 361 LIGRGTFGSVYLATNSETGALCAMKEVDLIPDDPKCAESIKQLEQEIKVLSQLKHPNIVQ 420

Query: 421 YYGSEIIDDHFYIYLEYVFPGSINKYVREHCGAITESIVRNFTRHILSGLAYLHSTKTIH 480
           YYGSEIIDDHFYIYLEYV+PGSINKYVRE CGAITESIVRNFTRHILSGLAYLHSTKTIH
Sbjct: 421 YYGSEIIDDHFYIYLEYVYPGSINKYVRERCGAITESIVRNFTRHILSGLAYLHSTKTIH 480

Query: 481 RDIKGANLLVDSSGVVKLADFGMAKHLTGQYDLSLKGSPYWMAPEVIKAAMLKDINPDLA 540
           RDIKGANLLVDSSGVVKLADFGMAKHLTGQYDLSLKGSPYWMAPEV+KAAMLKD NP+LA
Sbjct: 481 RDIKGANLLVDSSGVVKLADFGMAKHLTGQYDLSLKGSPYWMAPEVMKAAMLKDANPNLA 540

Query: 541 LAVDIWSLGCTIIEMLNGKPPWSEYEGHQAMFKVLNKTPPIPEKLSPEGKDFLQCCFQRN 600
           LAVDIWSLGC IIEML+GKPPWSE+EGHQAMFKVLNKTPP+PEKLSPEGKDFLQCCFQRN
Sbjct: 541 LAVDIWSLGCAIIEMLDGKPPWSEFEGHQAMFKVLNKTPPVPEKLSPEGKDFLQCCFQRN 600

Query: 601 PADRPTAMVLLDHPFLRFSNDSNVSIPTSAFSTMNLLEKLLSPKDHLHPKGDQGQNSPGT 660
           PADRPTAM LLDHPFLR SNDSN+S PTSAF TMN +EKLLSPKDH  PKGDQ QNS GT
Sbjct: 601 PADRPTAMALLDHPFLRSSNDSNISTPTSAFLTMNRMEKLLSPKDHRSPKGDQAQNSSGT 660

Query: 661 SAANDYLPCRSPSTNIPSNIPASAVNNHHFALSRTHTREVPHL 704
            A N YL CRSPSTNI SNIPASAVNNH FA+SRTH REVPHL
Sbjct: 661 LAENVYLSCRSPSTNISSNIPASAVNNHPFAVSRTHKREVPHL 703

BLAST of Lag0004325 vs. TAIR 10
Match: AT5G66850.1 (mitogen-activated protein kinase kinase kinase 5 )

HSP 1 Score: 501.1 bits (1289), Expect = 1.4e-141
Identity = 328/688 (47.67%), Postives = 401/688 (58.28%), Query Frame = 0

Query: 34  RLTRQRKLRHLTDQDVGLQHRRSFPDSPD-----------LSAKPRSPLGGSERWSSEAL 93
           RLTRQRKLRHLTD DV L  RR+   S              +A PRSP       S+  L
Sbjct: 52  RLTRQRKLRHLTDDDVLLGERRASTSSSTFDSGLTRSPSAFTAVPRSP-------SAVPL 111

Query: 94  PQPLPLPEYFPLRNPESGSWSSGQGRVGSPIEAPVRKSSDHSTTNANRSLGNNQRRVAID 153
           P PLPLPE   +RN  +      + R    + +  R SS    T+ N     + R+   +
Sbjct: 112 PLPLPLPEVAGIRNAANARGLDDRDRDPERLISD-RTSSGPPLTSVNGGFARDSRKATEN 171

Query: 154 FALE---VANGNTGGNGATTFTIPQSLSVNSGKSNSNKEFVFVCLDQPPNNKGRGSPTHP 213
            + +     N N       T + P S  ++   S              P  K  G     
Sbjct: 172 SSYQDFSPRNRNGYWVNIPTMSAPTSPYMSPVPS--------------PQRKSTGH---- 231

Query: 214 SVERGNFNLTPKTSSSKSAPTSILPSPAVSPRRSYTGDLFVSGLTRHEHQESPSNNSPKV 273
             +   F L PK++ + SAP   L +  + P   Y    F    T +    SP   SP+ 
Sbjct: 232 --DLPFFYLPPKSNQAWSAPDMPLDTSGLPPPAFYDITAF---STDNSPIHSPQPRSPR- 291

Query: 274 PTLKTAHSSQPSPLHSPTTRSSASNSRSPND-MPFPLHHKLQKDSSIDRSENHGHVNAHP 333
              K   S QPS   SP    S  +S  P D +  PLH +L  D +  R +     N HP
Sbjct: 292 ---KQIRSPQPSRPSSPL--HSVDSSAPPRDSVSSPLHPRLSTDVTNGRRD---CCNVHP 351

Query: 334 LPLPPPLIACSQSSPQSHSSNVHHVIEKPFISSMKCQWQKGKLIGRGTFGSVYLATNRET 393
           LPLPP    CS SS  S  S    +    F   M  QW+KGKLIGRGTFGSVY+A+N ET
Sbjct: 352 LPLPPG-ATCSSSSAASVPSPQAPLKLDSF--PMNSQWKKGKLIGRGTFGSVYVASNSET 411

Query: 394 GALCAMKEVDLIPDDPKSAESIKQLEQEIKVLSQLKHPNIVQYYGSEIIDDHFYIYLEYV 453
           GALCAMKEV+L PDDPKSAE IKQLEQEIK+LS L+HPNIVQY+GSE ++D F+IYLEYV
Sbjct: 412 GALCAMKEVELFPDDPKSAECIKQLEQEIKLLSNLQHPNIVQYFGSETVEDRFFIYLEYV 471

Query: 454 FPGSINKYVREHCGAITESIVRNFTRHILSGLAYLHSTKTIHRDIKGANLLVDSSGVVKL 513
            PGSINKY+R+HCG +TES+VRNFTRHILSGLAYLH+ KT+HRDIKGANLLVD+SGVVKL
Sbjct: 472 HPGSINKYIRDHCGTMTESVVRNFTRHILSGLAYLHNKKTVHRDIKGANLLVDASGVVKL 531

Query: 514 ADFGMAKHLTGQ-YDLSLKGSPYWMAPEVIKAAMLKDINPDLALAVDIWSLGCTIIEMLN 573
           ADFGMAKHLTGQ  DLSLKGSPYWMAPE+++A M KD NPDLA AVDIWSLGCTIIEM  
Sbjct: 532 ADFGMAKHLTGQRADLSLKGSPYWMAPELMQAVMQKDSNPDLAFAVDIWSLGCTIIEMFT 591

Query: 574 GKPPWSEYEGHQAMFKVLNKTPPIPEKLSPEGKDFLQCCFQRNPADRPTAMVLLDHPFLR 633
           GKPPWSE+EG  AMFKV+  +PPIPE +SPEGKDFL+ CFQRNPA+RPTA +LL+H FL+
Sbjct: 592 GKPPWSEFEGAAAMFKVMRDSPPIPESMSPEGKDFLRLCFQRNPAERPTASMLLEHRFLK 651

Query: 634 FS---------------NDSNVSIPTSAFSTMNL-LEKLLSPKDHLHPKGDQGQ------ 683
            S               N  N++ P+S     N  L+++   ++    + + GQ      
Sbjct: 652 NSLQPTSPSNSDVSQLFNGMNITEPSSRREKPNFKLDQVPRARNMTSSESESGQQQQQQQ 696

BLAST of Lag0004325 vs. TAIR 10
Match: AT1G63700.1 (Protein kinase superfamily protein )

HSP 1 Score: 339.3 bits (869), Expect = 7.1e-93
Identity = 267/679 (39.32%), Postives = 352/679 (51.84%), Query Frame = 0

Query: 1   MPFFYKSASNSRIQQK--VPDSEFSVGLPEPLVQRRLTRQRKLRHLTDQDVGLQHRRSFP 60
           MP++ KS    +   K  + D+ F+  L      R   R RK R   D+ V    R +  
Sbjct: 1   MPWWSKSKDEKKKTNKESIIDA-FNRKLGFASEDRSSGRSRKSRRRRDEIV--SERGAIS 60

Query: 61  DSPDLSAKPRSPLGG----SERWSSEALPQPLPLPEYFPLRNPESGSWSSGQGRVGSPIE 120
             P  S  P + +      +ER  +  LP+P+  P      +  +GS   G      P  
Sbjct: 61  RLPSRSPSPSTRVSRCQSFAERSPAVPLPRPIVRPHVTSTDSGMNGSQRPGLDANLKPSW 120

Query: 121 APVRKSSDHSTTNANRSLGNNQRRVAIDFA-LEVANGNTGG----------------NGA 180
            P+ K   H  T+   + G        DFA   V++G++ G                NG 
Sbjct: 121 LPLPK--PHGATSIPDNTGAEP-----DFATASVSSGSSVGDIPSDSLLSPLASDCENGN 180

Query: 181 TTFTIPQSLSVNSGKSNSNKEFVFVCLDQPPNNK-----GRGSPTHPSVERGNFNLTPKT 240
            T   P ++S      +SNK    +    P  N+      R  P    V+  N  +  + 
Sbjct: 181 RT---PVNISSRDQSMHSNKNSAEMFKPVPNKNRILSASPRRRPLGTHVK--NLQIPQRD 240

Query: 241 SSSKSAPTSILPSPAVSPRRSYTGD------LFVS------GLTRHEHQESP------SN 300
               SAP S+L SP+ SP RS+  D      L +S       L       SP       N
Sbjct: 241 LVLCSAPDSLLSSPSRSPMRSFIPDQVSNHGLLISKPYSDVSLLGSGQCSSPGSGYNSGN 300

Query: 301 NS------PKVPTLKTAHSSQPSPLHSPTTRSSASNSRSPNDMPFPLHHKL--QKDSSID 360
           NS       ++   ++  S + SP+ SP   S   +SR  +    PLH +       S  
Sbjct: 301 NSIGGDMATQLFWPQSRCSPECSPVPSPRMTSPGPSSRIQSGAVTPLHPRAGGSTTGSPT 360

Query: 361 RSENHGHVNAHPLPLPPPLIA-CSQSSPQSHSSNVHHVIEKPFIS----SMKCQWQKGKL 420
           R  +     +H LPLPP LI+     SP   ++    V   P  +    S   +W+KG+L
Sbjct: 361 RRLDDNRQQSHRLPLPPLLISNTCPFSPTYSAATSPSVPRSPARAEATVSPGSRWKKGRL 420

Query: 421 IGRGTFGSVYLATNRETGALCAMKEVDLIPDDPKSAESIKQLEQEIKVLSQLKHPNIVQY 480
           +G G+FG VYL  N E+G +CAMKEV L  DDPKS ES +QL QEI VLS+L+H NIVQY
Sbjct: 421 LGMGSFGHVYLGFNSESGEMCAMKEVTLCSDDPKSRESAQQLGQEISVLSRLRHQNIVQY 480

Query: 481 YGSEIIDDHFYIYLEYVFPGSINKYVREHCGAITESIVRNFTRHILSGLAYLHSTKTIHR 540
           YGSE +DD  YIYLEYV  GSI K ++E+ G   E+ +RN+T+ ILSGLAYLH+  T+HR
Sbjct: 481 YGSETVDDKLYIYLEYVSGGSIYKLLQEY-GQFGENAIRNYTQQILSGLAYLHAKNTVHR 540

Query: 541 DIKGANLLVDSSGVVKLADFGMAKHLTGQY-DLSLKGSPYWMAPEVIKAAMLKDINPDLA 600
           DIKGAN+LVD  G VK+ADFGMAKH+T Q   LS KGSPYWMAPEVIK +   +      
Sbjct: 541 DIKGANILVDPHGRVKVADFGMAKHITAQSGPLSFKGSPYWMAPEVIKNSNGSN------ 600

Query: 601 LAVDIWSLGCTIIEMLNGKPPWSEYEGHQAMFKVLN--KTPPIPEKLSPEGKDFLQCCFQ 618
           LAVDIWSLGCT++EM   KPPWS+YEG  AMFK+ N  + P IP+ LS EGKDF++ C Q
Sbjct: 601 LAVDIWSLGCTVLEMATTKPPWSQYEGVPAMFKIGNSKELPDIPDHLSEEGKDFVRKCLQ 657

BLAST of Lag0004325 vs. TAIR 10
Match: AT1G53570.2 (mitogen-activated protein kinase kinase kinase 3 )

HSP 1 Score: 320.5 bits (820), Expect = 3.4e-87
Identity = 177/360 (49.17%), Postives = 226/360 (62.78%), Query Frame = 0

Query: 294 PLHHKLQKDSSIDRS-----ENHGHVNAHPLPLPPPLIACSQSSPQS-HSSNVHHVIEKP 353
           P H +L    S++ S     +       HPLP PP     S +SP + H S +    E  
Sbjct: 152 PRHQRLSGVVSLESSTGRNDDGRSSSECHPLPRPP----TSPTSPSAVHGSRIGGGYETS 211

Query: 354 FISSMKCQWQKGKLIGRGTFGSVYLATNRETGALCAMKEVDLIPDDPKSAESIKQLEQEI 413
              S    W+KGK +G GTFG VYL  N E G +CA+KEV +I DD  S E +KQL QEI
Sbjct: 212 --PSGFSTWKKGKFLGSGTFGQVYLGFNSEKGKMCAIKEVKVISDDQTSKECLKQLNQEI 271

Query: 414 KVLSQLKHPNIVQYYGSEIIDDHFYIYLEYVFPGSINKYVREHCGAITESIVRNFTRHIL 473
            +L+QL HPNIVQYYGSE+ ++   +YLEYV  GSI+K ++++ G+ TE +++N+TR IL
Sbjct: 272 NLLNQLCHPNIVQYYGSELSEETLSVYLEYVSGGSIHKLLKDY-GSFTEPVIQNYTRQIL 331

Query: 474 SGLAYLHSTKTIHRDIKGANLLVDSSGVVKLADFGMAKHLTG-QYDLSLKGSPYWMAPEV 533
           +GLAYLH   T+HRDIKGAN+LVD +G +KLADFGMAKH+T     LS KGSPYWMAPEV
Sbjct: 332 AGLAYLHGRNTVHRDIKGANILVDPNGEIKLADFGMAKHVTAFSTMLSFKGSPYWMAPEV 391

Query: 534 IKAAMLKDINPDLALAVDIWSLGCTIIEMLNGKPPWSEYEGHQAMFKVLNK--TPPIPEK 593
           + +            AVDIWSLGCTI+EM   KPPWS++EG  A+FK+ N   TP IP+ 
Sbjct: 392 VMS------QNGYTHAVDIWSLGCTILEMATSKPPWSQFEGVAAIFKIGNSKDTPEIPDH 451

Query: 594 LSPEGKDFLQCCFQRNPADRPTAMVLLDHPFLR----------------FSNDSNVSIPT 629
           LS + K+F++ C QRNP  RPTA  LL+HPFLR                 S D N S+PT
Sbjct: 452 LSNDAKNFIRLCLQRNPTVRPTASQLLEHPFLRNTTRVASTSLPKDFPPRSYDGNFSLPT 498

BLAST of Lag0004325 vs. TAIR 10
Match: AT1G53570.3 (mitogen-activated protein kinase kinase kinase 3 )

HSP 1 Score: 320.5 bits (820), Expect = 3.4e-87
Identity = 177/360 (49.17%), Postives = 226/360 (62.78%), Query Frame = 0

Query: 294 PLHHKLQKDSSIDRS-----ENHGHVNAHPLPLPPPLIACSQSSPQS-HSSNVHHVIEKP 353
           P H +L    S++ S     +       HPLP PP     S +SP + H S +    E  
Sbjct: 152 PRHQRLSGVVSLESSTGRNDDGRSSSECHPLPRPP----TSPTSPSAVHGSRIGGGYETS 211

Query: 354 FISSMKCQWQKGKLIGRGTFGSVYLATNRETGALCAMKEVDLIPDDPKSAESIKQLEQEI 413
              S    W+KGK +G GTFG VYL  N E G +CA+KEV +I DD  S E +KQL QEI
Sbjct: 212 --PSGFSTWKKGKFLGSGTFGQVYLGFNSEKGKMCAIKEVKVISDDQTSKECLKQLNQEI 271

Query: 414 KVLSQLKHPNIVQYYGSEIIDDHFYIYLEYVFPGSINKYVREHCGAITESIVRNFTRHIL 473
            +L+QL HPNIVQYYGSE+ ++   +YLEYV  GSI+K ++++ G+ TE +++N+TR IL
Sbjct: 272 NLLNQLCHPNIVQYYGSELSEETLSVYLEYVSGGSIHKLLKDY-GSFTEPVIQNYTRQIL 331

Query: 474 SGLAYLHSTKTIHRDIKGANLLVDSSGVVKLADFGMAKHLTG-QYDLSLKGSPYWMAPEV 533
           +GLAYLH   T+HRDIKGAN+LVD +G +KLADFGMAKH+T     LS KGSPYWMAPEV
Sbjct: 332 AGLAYLHGRNTVHRDIKGANILVDPNGEIKLADFGMAKHVTAFSTMLSFKGSPYWMAPEV 391

Query: 534 IKAAMLKDINPDLALAVDIWSLGCTIIEMLNGKPPWSEYEGHQAMFKVLNK--TPPIPEK 593
           + +            AVDIWSLGCTI+EM   KPPWS++EG  A+FK+ N   TP IP+ 
Sbjct: 392 VMS------QNGYTHAVDIWSLGCTILEMATSKPPWSQFEGVAAIFKIGNSKDTPEIPDH 451

Query: 594 LSPEGKDFLQCCFQRNPADRPTAMVLLDHPFLR----------------FSNDSNVSIPT 629
           LS + K+F++ C QRNP  RPTA  LL+HPFLR                 S D N S+PT
Sbjct: 452 LSNDAKNFIRLCLQRNPTVRPTASQLLEHPFLRNTTRVASTSLPKDFPPRSYDGNFSLPT 498

BLAST of Lag0004325 vs. TAIR 10
Match: AT1G53570.1 (mitogen-activated protein kinase kinase kinase 3 )

HSP 1 Score: 320.1 bits (819), Expect = 4.5e-87
Identity = 171/333 (51.35%), Postives = 219/333 (65.77%), Query Frame = 0

Query: 294 PLHHKLQKDSSIDRS-----ENHGHVNAHPLPLPPPLIACSQSSPQS-HSSNVHHVIEKP 353
           P H +L    S++ S     +       HPLP PP     S +SP + H S +    E  
Sbjct: 152 PRHQRLSGVVSLESSTGRNDDGRSSSECHPLPRPP----TSPTSPSAVHGSRIGGGYETS 211

Query: 354 FISSMKCQWQKGKLIGRGTFGSVYLATNRETGALCAMKEVDLIPDDPKSAESIKQLEQEI 413
              S    W+KGK +G GTFG VYL  N E G +CA+KEV +I DD  S E +KQL QEI
Sbjct: 212 --PSGFSTWKKGKFLGSGTFGQVYLGFNSEKGKMCAIKEVKVISDDQTSKECLKQLNQEI 271

Query: 414 KVLSQLKHPNIVQYYGSEIIDDHFYIYLEYVFPGSINKYVREHCGAITESIVRNFTRHIL 473
            +L+QL HPNIVQYYGSE+ ++   +YLEYV  GSI+K ++++ G+ TE +++N+TR IL
Sbjct: 272 NLLNQLCHPNIVQYYGSELSEETLSVYLEYVSGGSIHKLLKDY-GSFTEPVIQNYTRQIL 331

Query: 474 SGLAYLHSTKTIHRDIKGANLLVDSSGVVKLADFGMAKHLTG-QYDLSLKGSPYWMAPEV 533
           +GLAYLH   T+HRDIKGAN+LVD +G +KLADFGMAKH+T     LS KGSPYWMAPEV
Sbjct: 332 AGLAYLHGRNTVHRDIKGANILVDPNGEIKLADFGMAKHVTAFSTMLSFKGSPYWMAPEV 391

Query: 534 IKAAMLKDINPDLALAVDIWSLGCTIIEMLNGKPPWSEYEGHQAMFKVLNK--TPPIPEK 593
           + +            AVDIWSLGCTI+EM   KPPWS++EG  A+FK+ N   TP IP+ 
Sbjct: 392 VMS------QNGYTHAVDIWSLGCTILEMATSKPPWSQFEGVAAIFKIGNSKDTPEIPDH 451

Query: 594 LSPEGKDFLQCCFQRNPADRPTAMVLLDHPFLR 618
           LS + K+F++ C QRNP  RPTA  LL+HPFLR
Sbjct: 452 LSNDAKNFIRLCLQRNPTVRPTASQLLEHPFLR 471

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
XP_038885756.10.0e+0086.22mitogen-activated protein kinase kinase kinase 5-like isoform X2 [Benincasa hisp... [more]
XP_038885754.10.0e+0085.25mitogen-activated protein kinase kinase kinase 5-like isoform X1 [Benincasa hisp... [more]
XP_008444652.10.0e+0084.49PREDICTED: mitogen-activated protein kinase kinase kinase YODA-like isoform X2 [... [more]
XP_008444651.10.0e+0083.54PREDICTED: mitogen-activated protein kinase kinase kinase YODA-like isoform X1 [... [more]
XP_022961725.10.0e+0084.94mitogen-activated protein kinase kinase kinase 5-like [Cucurbita moschata][more]
Match NameE-valueIdentityDescription
Q9C5H52.0e-14047.67Mitogen-activated protein kinase kinase kinase 5 OS=Arabidopsis thaliana OX=3702... [more]
Q9CAD51.0e-9139.32Mitogen-activated protein kinase kinase kinase YODA OS=Arabidopsis thaliana OX=3... [more]
F4HRJ46.3e-8651.35Mitogen-activated protein kinase kinase kinase 3 OS=Arabidopsis thaliana OX=3702... [more]
Q54R821.7e-6242.81Mitogen-activated protein kinase kinase kinase A OS=Dictyostelium discoideum OX=... [more]
Q9FZ363.2e-6140.87Mitogen-activated protein kinase kinase kinase 2 OS=Arabidopsis thaliana OX=3702... [more]
Match NameE-valueIdentityDescription
A0A1S3BBK60.0e+0084.49mitogen-activated protein kinase kinase kinase YODA-like isoform X2 OS=Cucumis m... [more]
A0A1S3BAV60.0e+0083.54mitogen-activated protein kinase kinase kinase YODA-like isoform X1 OS=Cucumis m... [more]
A0A0A0LLB60.0e+0084.56Protein kinase domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_2G360... [more]
A0A6J1HD450.0e+0084.94mitogen-activated protein kinase kinase kinase 5-like OS=Cucurbita moschata OX=3... [more]
A0A6J1K5B80.0e+0083.93mitogen-activated protein kinase kinase kinase 5-like OS=Cucurbita maxima OX=366... [more]
Match NameE-valueIdentityDescription
AT5G66850.11.4e-14147.67mitogen-activated protein kinase kinase kinase 5 [more]
AT1G63700.17.1e-9339.32Protein kinase superfamily protein [more]
AT1G53570.23.4e-8749.17mitogen-activated protein kinase kinase kinase 3 [more]
AT1G53570.33.4e-8749.17mitogen-activated protein kinase kinase kinase 3 [more]
AT1G53570.14.5e-8751.35mitogen-activated protein kinase kinase kinase 3 [more]
InterPro
Analysis Name: InterPro Annotations of Sponge gourd (AG-4) v1
Date Performed: 2022-08-01
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR000719Protein kinase domainSMARTSM00220serkin_6coord: 356..616
e-value: 1.3E-94
score: 330.3
IPR000719Protein kinase domainPFAMPF00069Pkinasecoord: 357..616
e-value: 5.9E-69
score: 232.4
IPR000719Protein kinase domainPROSITEPS50011PROTEIN_KINASE_DOMcoord: 356..616
score: 51.367916
NoneNo IPR availableGENE3D1.10.510.10Transferase(Phosphotransferase) domain 1coord: 353..645
e-value: 4.1E-82
score: 277.5
NoneNo IPR availablePIRSRPIRSR630220-2PIRSR630220-2coord: 362..612
e-value: 1.8E-20
score: 70.6
NoneNo IPR availablePIRSRPIRSR000550-2PIRSR000550-2coord: 355..604
e-value: 1.8E-32
score: 110.4
NoneNo IPR availablePIRSRPIRSR037993-2PIRSR037993-2coord: 350..620
e-value: 1.6E-21
score: 74.4
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 653..681
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 295..313
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 190..228
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 644..681
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 37..134
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 184..337
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 41..61
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 118..134
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 246..290
NoneNo IPR availablePANTHERPTHR48016MAP KINASE KINASE KINASE SSK2-RELATED-RELATEDcoord: 1..693
NoneNo IPR availablePANTHERPTHR48016:SF35SUBFAMILY NOT NAMEDcoord: 1..693
IPR017441Protein kinase, ATP binding sitePROSITEPS00107PROTEIN_KINASE_ATPcoord: 362..385
IPR011009Protein kinase-like domain superfamilySUPERFAMILY56112Protein kinase-like (PK-like)coord: 354..628

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Lag0004325.1Lag0004325.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0006468 protein phosphorylation
molecular_function GO:0005524 ATP binding
molecular_function GO:0004672 protein kinase activity