Lag0004301 (gene) Sponge gourd (AG‐4) v1

Overview
NameLag0004301
Typegene
OrganismLuffa acutangula (Sponge gourd (AG‐4) v1)
Descriptioncalmodulin-like
Locationchr6: 2784109 .. 2784642 (+)
RNA-Seq ExpressionLag0004301
SyntenyLag0004301
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonCDSpolypeptide
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGTTTAGCTCCCAACTTCGAGATCGCAGGGCAAAGGATGAAGCAAATGAATTGATCAAGAGGTATGATAAAAATGGTGACGGTGTTCTTACTAAAGAGGAGCTAGTTGGATTCTTCTTCCACGCCGCTCGAGGTTCAACTCAACTCAACAATCAAGGATGTGTATCTAAAATCCCAAAAACTAACGACAATGTTATACACAAGCTTACTTCAAATGTTCATATAAAAGCAAAGAAGCAGTCACAAGACAAAGCTCAAAAGCTATCAACAAGAGTTCTTGATGGAAAGACATCTGATATGCCACTCAGTCGAGAGGAGATGAAGGATATTTTCAAATATCATGATAGTGACAAGGATGGTTTTCTCAACATGAGGGAGCTAACAAAAGCTTTCAGTTTTCTAGGTTCCATTATTCCATTCTACAAAGCTCACTATGGTTTGGCTTATGCTGATGTTAATAAAGATGGTCTTATCAGTGAGGCTGAGCTTGACAAACTTGTCGATTATGCTTATAAAATCATCCCGAAGAAATAA

mRNA sequence

ATGTTTAGCTCCCAACTTCGAGATCGCAGGGCAAAGGATGAAGCAAATGAATTGATCAAGAGGTATGATAAAAATGGTGACGGTGTTCTTACTAAAGAGGAGCTAGTTGGATTCTTCTTCCACGCCGCTCGAGGTTCAACTCAACTCAACAATCAAGGATGTGTATCTAAAATCCCAAAAACTAACGACAATGTTATACACAAGCTTACTTCAAATGTTCATATAAAAGCAAAGAAGCAGTCACAAGACAAAGCTCAAAAGCTATCAACAAGAGTTCTTGATGGAAAGACATCTGATATGCCACTCAGTCGAGAGGAGATGAAGGATATTTTCAAATATCATGATAGTGACAAGGATGGTTTTCTCAACATGAGGGAGCTAACAAAAGCTTTCAGTTTTCTAGGTTCCATTATTCCATTCTACAAAGCTCACTATGGTTTGGCTTATGCTGATGTTAATAAAGATGGTCTTATCAGTGAGGCTGAGCTTGACAAACTTGTCGATTATGCTTATAAAATCATCCCGAAGAAATAA

Coding sequence (CDS)

ATGTTTAGCTCCCAACTTCGAGATCGCAGGGCAAAGGATGAAGCAAATGAATTGATCAAGAGGTATGATAAAAATGGTGACGGTGTTCTTACTAAAGAGGAGCTAGTTGGATTCTTCTTCCACGCCGCTCGAGGTTCAACTCAACTCAACAATCAAGGATGTGTATCTAAAATCCCAAAAACTAACGACAATGTTATACACAAGCTTACTTCAAATGTTCATATAAAAGCAAAGAAGCAGTCACAAGACAAAGCTCAAAAGCTATCAACAAGAGTTCTTGATGGAAAGACATCTGATATGCCACTCAGTCGAGAGGAGATGAAGGATATTTTCAAATATCATGATAGTGACAAGGATGGTTTTCTCAACATGAGGGAGCTAACAAAAGCTTTCAGTTTTCTAGGTTCCATTATTCCATTCTACAAAGCTCACTATGGTTTGGCTTATGCTGATGTTAATAAAGATGGTCTTATCAGTGAGGCTGAGCTTGACAAACTTGTCGATTATGCTTATAAAATCATCCCGAAGAAATAA

Protein sequence

MFSSQLRDRRAKDEANELIKRYDKNGDGVLTKEELVGFFFHAARGSTQLNNQGCVSKIPKTNDNVIHKLTSNVHIKAKKQSQDKAQKLSTRVLDGKTSDMPLSREEMKDIFKYHDSDKDGFLNMRELTKAFSFLGSIIPFYKAHYGLAYADVNKDGLISEAELDKLVDYAYKIIPKK
Homology
BLAST of Lag0004301 vs. NCBI nr
Match: XP_022951742.1 (probable calcium-binding protein CML11 [Cucurbita moschata])

HSP 1 Score: 224.2 bits (570), Expect = 9.1e-55
Identity = 116/167 (69.46%), Postives = 134/167 (80.24%), Query Frame = 0

Query: 11  AKDEANELIKRYDKNGDGVLTKEELVGFFFHAARGSTQLNNQGCVSKIPKTNDNVIHKLT 70
           A+++ANELIK+YDKNGDGVLTKEEL   F    RGSTQLNNQG +SKIP+TND   HKL 
Sbjct: 10  AENKANELIKKYDKNGDGVLTKEEL-QVFLRDVRGSTQLNNQGFLSKIPRTNDKGAHKLN 69

Query: 71  SNVHIKAKKQSQDKAQKLSTRVLDGKTSDMPLSREEMKDIFKYHDSDKDGFLNMRELTKA 130
           SN     K Q Q KA K ST+VL  K S++PLSRE++K IFKY+DSDKDGFLN+RE+TKA
Sbjct: 70  SNPRTNPKIQVQSKATKSSTKVLSEKVSNVPLSREQIKKIFKYYDSDKDGFLNIREVTKA 129

Query: 131 FSFLGSIIPFYKAHYGLAYADVNKDGLISEAELDKLVDYAYKIIPKK 178
           FSFLGSI PFYKA Y + +AD N DGLISEAEL+KL+DYAYKII KK
Sbjct: 130 FSFLGSINPFYKAQYAMTFADANNDGLISEAELEKLIDYAYKIIKKK 175

BLAST of Lag0004301 vs. NCBI nr
Match: XP_023002692.1 (probable calcium-binding protein CML14 [Cucurbita maxima])

HSP 1 Score: 224.2 bits (570), Expect = 9.1e-55
Identity = 113/167 (67.66%), Postives = 136/167 (81.44%), Query Frame = 0

Query: 11  AKDEANELIKRYDKNGDGVLTKEELVGFFFHAARGSTQLNNQGCVSKIPKTNDNVIHKLT 70
           A++EANEL+K+YD+NGDGVLTK+EL   F    RGSTQLNNQG +S+IPKTNDNV HKL+
Sbjct: 10  AENEANELMKKYDRNGDGVLTKKEL-QIFLRDVRGSTQLNNQGFLSRIPKTNDNVAHKLS 69

Query: 71  SNVHIKAKKQSQDKAQKLSTRVLDGKTSDMPLSREEMKDIFKYHDSDKDGFLNMRELTKA 130
           SN H   K Q Q+KA K ST+V     SD+ LSRE++K IFKY+DSDKDGFLN+ E+TKA
Sbjct: 70  SNAHSNPKMQVQEKATKSSTKVFSENVSDVRLSREQIKKIFKYYDSDKDGFLNITEVTKA 129

Query: 131 FSFLGSIIPFYKAHYGLAYADVNKDGLISEAELDKLVDYAYKIIPKK 178
           FSFLGSI PFYKA Y +++AD N DG+ISEAEL+KL+DYA KIIPKK
Sbjct: 130 FSFLGSINPFYKAQYAISFADANNDGVISEAELEKLIDYACKIIPKK 175

BLAST of Lag0004301 vs. NCBI nr
Match: XP_022951134.1 (uncharacterized protein LOC111454074 isoform X1 [Cucurbita moschata])

HSP 1 Score: 223.4 bits (568), Expect = 1.5e-54
Identity = 116/168 (69.05%), Postives = 138/168 (82.14%), Query Frame = 0

Query: 11  AKDEANELIKRYDKNGDGVLTKEELVGFFFHAARGSTQL-NNQGCVSKIPKTNDNVIHKL 70
           A+DEAN L+K+YD NGDG+LTKEEL   F H ARGSTQL NNQG VSKIPK ++NV HK 
Sbjct: 10  AEDEANRLMKKYDTNGDGMLTKEEL-QVFLHKARGSTQLNNNQGFVSKIPKASNNVAHKH 69

Query: 71  TSNVHIKAKKQSQDKAQKLSTRVLDGKTSDMPLSREEMKDIFKYHDSDKDGFLNMRELTK 130
           +SN H+K KKQ QDK +K ST+ LDG+ S + LSRE+MK+IFK HDS+KDGFL+++E+TK
Sbjct: 70  SSNSHMKHKKQVQDKGKKSSTKFLDGEMSKLQLSREQMKEIFKNHDSNKDGFLDIKEVTK 129

Query: 131 AFSFLGSIIPFYKAHYGLAYADVNKDGLISEAELDKLVDYAYKIIPKK 178
           AFSFLGSI+PF KAHY +AYAD N DGLISE ELDKL+DYA K I KK
Sbjct: 130 AFSFLGSILPFNKAHYAMAYADTNHDGLISEFELDKLIDYASKFINKK 176

BLAST of Lag0004301 vs. NCBI nr
Match: XP_023538396.1 (probable calcium-binding protein CML11 isoform X1 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 223.0 bits (567), Expect = 2.0e-54
Identity = 116/167 (69.46%), Postives = 133/167 (79.64%), Query Frame = 0

Query: 11  AKDEANELIKRYDKNGDGVLTKEELVGFFFHAARGSTQLNNQGCVSKIPKTNDNVIHKLT 70
           A++EANEL+K+YD+NGDGVLTKEEL  FF    RGSTQLNNQG +SKIPKTND   HKL+
Sbjct: 10  AENEANELMKKYDRNGDGVLTKEEL-QFFLRDVRGSTQLNNQGFLSKIPKTNDKGAHKLS 69

Query: 71  SNVHIKAKKQSQDKAQKLSTRVLDGKTSDMPLSREEMKDIFKYHDSDKDGFLNMRELTKA 130
           SN H   K Q Q KA   ST+V   K S++PLSRE++K IFKY+DSDKDGFLN RE+TKA
Sbjct: 70  SNAHTNPKIQVQGKATNSSTKVFGEKVSNVPLSREQIKKIFKYYDSDKDGFLNRREVTKA 129

Query: 131 FSFLGSIIPFYKAHYGLAYADVNKDGLISEAELDKLVDYAYKIIPKK 178
           FSFLGSI PFYKA Y + +AD N DGLISEAEL+KLVDYA KII KK
Sbjct: 130 FSFLGSINPFYKAQYAMTFADANNDGLISEAELEKLVDYAGKIIQKK 175

BLAST of Lag0004301 vs. NCBI nr
Match: XP_023538534.1 (putative calmodulin-like protein 6 isoform X1 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 221.9 bits (564), Expect = 4.5e-54
Identity = 114/168 (67.86%), Postives = 137/168 (81.55%), Query Frame = 0

Query: 11  AKDEANELIKRYDKNGDGVLTKEELVGFFFHAARGSTQL-NNQGCVSKIPKTNDNVIHKL 70
           A+DEAN L+K+YD NGDG+LTKEEL   F H ARGSTQL NNQG VSKIPK ++NV+HK 
Sbjct: 10  AEDEANRLMKKYDTNGDGMLTKEEL-QVFLHKARGSTQLNNNQGFVSKIPKASNNVVHKH 69

Query: 71  TSNVHIKAKKQSQDKAQKLSTRVLDGKTSDMPLSREEMKDIFKYHDSDKDGFLNMRELTK 130
           +SN H+K KKQ QDK +K ST+ LDG+ S + LSRE+MK+IFK HDS+KDGFL+++E+TK
Sbjct: 70  SSNSHMKHKKQVQDKGKKSSTKFLDGEMSKLQLSREQMKEIFKNHDSNKDGFLDIKEVTK 129

Query: 131 AFSFLGSIIPFYKAHYGLAYADVNKDGLISEAELDKLVDYAYKIIPKK 178
           AF FLGSI+PF KAHY + YAD N DGLISE ELDKL+DYA K I KK
Sbjct: 130 AFGFLGSILPFNKAHYAMTYADTNHDGLISEFELDKLIDYASKFINKK 176

BLAST of Lag0004301 vs. ExPASy Swiss-Prot
Match: P25071 (Calmodulin-like protein 12 OS=Arabidopsis thaliana OX=3702 GN=CML12 PE=1 SV=3)

HSP 1 Score: 55.5 bits (132), Expect = 7.3e-07
Identity = 40/150 (26.67%), Postives = 74/150 (49.33%), Query Frame = 0

Query: 14  EANELIKRYDKNGDGVLTKEELVGFFFHAARGSTQLNNQGCVSKIPKTNDNVIHKLTSNV 73
           E  E  + +DKNGDG +TK+EL    F   +  T+ + Q  ++++    D  I      +
Sbjct: 101 EYRESFRLFDKNGDGSITKKELRTVMFSLGKNRTKADLQDMMNEVDLDGDGTI-DFPEFL 160

Query: 74  HIKAKKQSQDKAQKLSTR-VLDGKTSDMPLSREEMKDIFKYHDSDKDGFLNMRELTKAFS 133
           ++ AK Q  D+A + + + ++D + +D  +   E ++ F+  D + DG++ + EL     
Sbjct: 161 YLMAKNQGHDQAPRHTKKTMVDYQLTDDQIL--EFREAFRVFDKNGDGYITVNELRTTMR 220

Query: 134 FLGSIIPFYKAHYGLAYADVNKDGLISEAE 163
            LG      +    +  AD + DG IS +E
Sbjct: 221 SLGETQTKAELQDMINEADADGDGTISFSE 247

BLAST of Lag0004301 vs. ExPASy Swiss-Prot
Match: O22845 (Calmodulin-like protein 5 OS=Arabidopsis thaliana OX=3702 GN=CML5 PE=2 SV=2)

HSP 1 Score: 52.0 bits (123), Expect = 8.1e-06
Identity = 45/138 (32.61%), Postives = 63/138 (45.65%), Query Frame = 0

Query: 3   SSQLRDRRAKDEANELIKRYDKNGDGVLTKEELVGFFFHAARGSTQLNNQGCVSKIP-KT 62
           SS    R    E   + + +DKNGDG +TKEEL          +  L N G    IP K 
Sbjct: 54  SSAPTKRIDPSELKRVFQMFDKNGDGRITKEEL----------NDSLENLGIY--IPDKD 113

Query: 63  NDNVIHKLTSN----VHIKAKKQSQDKAQKLSTRVLDGKTSDMPLSREEMKDIFKYHDSD 122
              +IHK+ +N    V I       D+ + L + ++D   +D     E+MKD F   D D
Sbjct: 114 LTQMIHKIDANGDGCVDI-------DEFESLYSSIVDEHHNDGETEEEDMKDAFNVFDQD 172

Query: 123 KDGFLNMRELTKAFSFLG 136
            DGF+ + EL    + LG
Sbjct: 174 GDGFITVEELKSVMASLG 172

BLAST of Lag0004301 vs. ExPASy Swiss-Prot
Match: P62344 (Calcium-dependent protein kinase 1 OS=Plasmodium falciparum (isolate 3D7) OX=36329 GN=CDPK1 PE=1 SV=2)

HSP 1 Score: 46.2 bits (108), Expect = 4.4e-04
Identity = 45/174 (25.86%), Postives = 77/174 (44.25%), Query Frame = 0

Query: 4   SQLRDRRAKDEANELIKRYDKNGDGVLTKEELVGFFFHAARGSTQLNNQGCVSKIPKTND 63
           S+L     + E  ++ K+ DKNGDG L K+EL+   ++  R     N  G +  + +  D
Sbjct: 366 SKLTTLEERKELTDIFKKLDKNGDGQLDKKELIE-GYNILRSFK--NELGELKNVEEEVD 425

Query: 64  NVIHKLTSNVHIKAKKQSQDKAQKLSTRVLDGKTSDMPLSREEMKDIFKYHDSDKDGFLN 123
           N++ +      +   K    +  +  +  +D     +  S E ++D F   D+DK G + 
Sbjct: 426 NILKE------VDFDKNGYIEYSEFISVCMD---KQILFSEERLRDAFNLFDTDKSGKIT 485

Query: 124 MRELTKAFSFLGSIIPFYKAHYGLAYADVNKDGLISEAELDKLVDYAYKIIPKK 178
             EL   F    + I     +  L  AD NKD +I   + D+ V+  +KI   K
Sbjct: 486 KEELANLFGL--TSISEQMWNEVLGEADKNKDNMI---DFDEFVNMMHKICDNK 522

BLAST of Lag0004301 vs. ExPASy Swiss-Prot
Match: P62343 (Calcium-dependent protein kinase 1 OS=Plasmodium falciparum (isolate K1 / Thailand) OX=5839 GN=CDPK1 PE=1 SV=2)

HSP 1 Score: 46.2 bits (108), Expect = 4.4e-04
Identity = 45/174 (25.86%), Postives = 77/174 (44.25%), Query Frame = 0

Query: 4   SQLRDRRAKDEANELIKRYDKNGDGVLTKEELVGFFFHAARGSTQLNNQGCVSKIPKTND 63
           S+L     + E  ++ K+ DKNGDG L K+EL+   ++  R     N  G +  + +  D
Sbjct: 366 SKLTTLEERKELTDIFKKLDKNGDGQLDKKELIE-GYNILRSFK--NELGELKNVEEEVD 425

Query: 64  NVIHKLTSNVHIKAKKQSQDKAQKLSTRVLDGKTSDMPLSREEMKDIFKYHDSDKDGFLN 123
           N++ +      +   K    +  +  +  +D     +  S E ++D F   D+DK G + 
Sbjct: 426 NILKE------VDFDKNGYIEYSEFISVCMD---KQILFSEERLRDAFNLFDTDKSGKIT 485

Query: 124 MRELTKAFSFLGSIIPFYKAHYGLAYADVNKDGLISEAELDKLVDYAYKIIPKK 178
             EL   F    + I     +  L  AD NKD +I   + D+ V+  +KI   K
Sbjct: 486 KEELANLFGL--TSISEQMWNEVLGEADKNKDNMI---DFDEFVNMMHKICDNK 522

BLAST of Lag0004301 vs. ExPASy Swiss-Prot
Match: A0A2I0BVG8 (Calcium-dependent protein kinase 1 OS=Plasmodium falciparum (isolate NF54) OX=5843 GN=PDCK1 PE=1 SV=1)

HSP 1 Score: 46.2 bits (108), Expect = 4.4e-04
Identity = 45/174 (25.86%), Postives = 77/174 (44.25%), Query Frame = 0

Query: 4   SQLRDRRAKDEANELIKRYDKNGDGVLTKEELVGFFFHAARGSTQLNNQGCVSKIPKTND 63
           S+L     + E  ++ K+ DKNGDG L K+EL+   ++  R     N  G +  + +  D
Sbjct: 366 SKLTTLEERKELTDIFKKLDKNGDGQLDKKELIE-GYNILRSFK--NELGELKNVEEEVD 425

Query: 64  NVIHKLTSNVHIKAKKQSQDKAQKLSTRVLDGKTSDMPLSREEMKDIFKYHDSDKDGFLN 123
           N++ +      +   K    +  +  +  +D     +  S E ++D F   D+DK G + 
Sbjct: 426 NILKE------VDFDKNGYIEYSEFISVCMD---KQILFSEERLRDAFNLFDTDKSGKIT 485

Query: 124 MRELTKAFSFLGSIIPFYKAHYGLAYADVNKDGLISEAELDKLVDYAYKIIPKK 178
             EL   F    + I     +  L  AD NKD +I   + D+ V+  +KI   K
Sbjct: 486 KEELANLFGL--TSISEQMWNEVLGEADKNKDNMI---DFDEFVNMMHKICDNK 522

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
XP_022951742.19.1e-5569.46probable calcium-binding protein CML11 [Cucurbita moschata][more]
XP_023002692.19.1e-5567.66probable calcium-binding protein CML14 [Cucurbita maxima][more]
XP_022951134.11.5e-5469.05uncharacterized protein LOC111454074 isoform X1 [Cucurbita moschata][more]
XP_023538396.12.0e-5469.46probable calcium-binding protein CML11 isoform X1 [Cucurbita pepo subsp. pepo][more]
XP_023538534.14.5e-5467.86putative calmodulin-like protein 6 isoform X1 [Cucurbita pepo subsp. pepo][more]
Match NameE-valueIdentityDescription
P250717.3e-0726.67Calmodulin-like protein 12 OS=Arabidopsis thaliana OX=3702 GN=CML12 PE=1 SV=3[more]
O228458.1e-0632.61Calmodulin-like protein 5 OS=Arabidopsis thaliana OX=3702 GN=CML5 PE=2 SV=2[more]
P623444.4e-0425.86Calcium-dependent protein kinase 1 OS=Plasmodium falciparum (isolate 3D7) OX=363... [more]
P623434.4e-0425.86Calcium-dependent protein kinase 1 OS=Plasmodium falciparum (isolate K1 / Thaila... [more]
A0A2I0BVG84.4e-0425.86Calcium-dependent protein kinase 1 OS=Plasmodium falciparum (isolate NF54) OX=58... [more]
InterPro
Analysis Name: InterPro Annotations of Sponge gourd (AG-4) v1
Date Performed: 2022-08-01
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
NoneNo IPR availablePRINTSPR00450RECOVERINcoord: 110..131
score: 32.17
coord: 156..174
score: 34.01
NoneNo IPR availableGENE3D1.10.238.10coord: 48..172
e-value: 3.6E-11
score: 45.3
NoneNo IPR availableGENE3D1.10.238.10coord: 1..39
e-value: 4.3E-6
score: 28.6
NoneNo IPR availablePANTHERPTHR10891:SF911SUBFAMILY NOT NAMEDcoord: 78..172
NoneNo IPR availablePANTHERPTHR10891EF-HAND CALCIUM-BINDING DOMAIN CONTAINING PROTEINcoord: 78..172
IPR002048EF-hand domainSMARTSM00054efh_1coord: 106..134
e-value: 2.3
score: 14.9
coord: 14..42
e-value: 0.008
score: 25.4
IPR002048EF-hand domainPFAMPF13202EF-hand_5coord: 18..35
e-value: 3.6E-4
score: 19.9
coord: 150..167
e-value: 0.013
score: 14.9
IPR002048EF-hand domainPFAMPF13405EF-hand_6coord: 106..135
e-value: 2.7E-6
score: 26.7
IPR002048EF-hand domainPROSITEPS50222EF_HAND_2coord: 102..137
score: 12.532831
IPR002048EF-hand domainPROSITEPS50222EF_HAND_2coord: 10..45
score: 12.616518
IPR018247EF-Hand 1, calcium-binding sitePROSITEPS00018EF_HAND_1coord: 23..35
IPR018247EF-Hand 1, calcium-binding sitePROSITEPS00018EF_HAND_1coord: 115..127
IPR018247EF-Hand 1, calcium-binding sitePROSITEPS00018EF_HAND_1coord: 151..163
IPR011992EF-hand domain pairSUPERFAMILY47473EF-handcoord: 9..166

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Lag0004301.1Lag0004301.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
molecular_function GO:0005509 calcium ion binding