Lag0004287 (gene) Sponge gourd (AG‐4) v1

Overview
NameLag0004287
Typegene
OrganismLuffa acutangula (Sponge gourd (AG‐4) v1)
DescriptionAbscisic acid 8'-hydroxylase
Locationchr6: 2614235 .. 2618031 (+)
RNA-Seq ExpressionLag0004287
SyntenyLag0004287
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonCDSpolypeptide
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGATGCCTCAAAATTTTCTACTCATTTCCATTTTTCCTCTTGCCCTCCTCCTTCTACCTCTAATTTTCTTTCATGTCCAAAAGATCAACAAGAATATTGCAAAGAAGCTCCCTCCTGGCTCAATGGGCTGGCCCCTAATCGGCGAGACCTTTCGTCTCTACTCTCAACATCCATCCTCCTTCTTCCTTGGAAAACAAAAAAGGTTATCTTGCTCTCTCGAGTACACGAAAAACCCTTTTCTCGAGAAGAAAATTATTAAAGAACTAAAATATTAGGAGATAATTAATTACTGTGAAGAAGAGACAGCCACATTGAATAATTTTTGCTGATGCAGGTATGGGGATATATTCAAGACCCACATTCTTGGGTGTCCGTGTGTGATGTTGGCGACTCCCGAGGCGGCTCGGTTCGTGCTAGTGACGCAGGCTCATCTCTTCAAGCCCACATACCCACAAAGCAAAGAGCGTCTAATCGGACCATCGGCTCTGTTTTTCCACCAAAGCGAAACCCATTTGCGGCTGCGTAAGCTAATCCAAAGCTCCCTCTCACCGGACCCTGTCCGAGCCCTGGTCCCTGACATCCACGCCCTCGCCCTCTCTACATTGCCCGCATGGCTCAATGGCCATCTCATCAACACTTTCCATCAAATGAAGAAGGTAAATTTCAAATTTTTTTATAAAAAAAAATCTAAATTTCAGTTGGGGCAGAGATGCTCTGTTAACAAAGACAAAAACAGAGCTTTCAAAAAGTTTGTGTTCTTAATTGAATCAAATGGGGACCAATTATTCCTTATCAATTGTTTAATTTTAGTATATTTTTCTTTTCCTTATGGTTGTGTCTTTTTTCTTCACTCTCCAAAGTTCTCTTTTGAAGTCGGAATTTTGGCGATTTTTGGGCATTTGAAGGACGCCAATTACAGAGACGAATTACTGAGAAACTATTCCATAGTGAACAAAGGCTACAATTCTTTTCCAACCAACATTCCCGGAACGCTTTACAAAAAAGCACTTAAAGTAAGTCAAAAACACACACATACAATACAATAACATTCGGATTCTCGAGTGAATTATTGTTTGTTTTTTCTAAGAATAATAATCTGTTTGCCACTTTTGTGTAAAGGCGAGAAAGAGGCTGGGAGAGATCATAAGCTGTATAATCTCAGAGAAAAAGGAGAAGAGAAAGGAATTGGTGGAGAAGAATTTGTTGGGTTGTTTGCTAAAATGGAGAGATGAAAATGGAGAAAGTTTAAGTGATGAACAGATTGCTGATAATATAATTGGAGTTCTGTTTGCTGCTCAGGACACCACTGCCAGTGTTCTGACATGGGTTCTCAAATATCTCCATGATAATCAGAAAGTTCTAGAAGCTGTCAAGGTACTCATCTTCTTCTTCTTCTTTTTTTGGACCTTATATAAGTTACTAACACTTTTTTGATGAGGTGAATTTTTAGTCACTATGAATAAAATTAGAGTATAGTTTGAAAAAAGAGAAAGAAAAGAAAGGAGAAGAGTGAATGCAATAGAAATAGATCATTAAGATCTGGATCTTGTGTGTGTTAACAAGTATATGTATTTATTATTATTATTATTATTTTTTACATAGAGGGGTTTGTTTGTGACATTGATTCTTTGTGGGTTTTTAAAGAAAAATTTAATTGTTATCAATGTGGGTATATAACTCTCTAACTTTCAATGTTTAATGAAGATTAAATGTCTAATTTATTGTGTTATGTTTGGATCCATGTTGTTGTAGGCAGAACAAAAGGCAATTGAAGAGTGTATTGATGAGGGGAGCCGACCAATGAGTTGGACTCAGACACGAAACATGTCGTTTACCAACAAGGTAGGAAGCTGGAGACTATTTATTTTTTGAAAAGAGATACTGGAGAAGTTGAACAAGTGTTTTTGAGAGATGGATCAAGTCAAAATTGAGCCTTTTTGTTTTCTATTTTTCTTATTTTTTGTTTTTTTAAAGTTATAGTATTCATTGATTGAGCTATTTGTCGGTTGCATTTAAAAAAATAAATAAAAATAGGTGGTGTTGGAGAGCTTGAGAATGGCGAGTATTATATCATTCACTTTTAGAGAAGCTGTGACAGATGTTGAATATAATGGTAAGACGCTAAAAATAACCCTAAATTAACATTAATATGTAAATGCACCTACCATATTTAACTTGTATTTGTTAGTTTGTTTTAATGTTCTTTTAAAATTAATAAAATATTTTGATATTTGGTTCTATATTTTTTAACACTAACATGTAAATGTACGTATTCAATGTTTTTTTCACAAAGCACTAGTTACAAGCACTTTTTTATTACTACCCTAATAGAGGCTCCTTTTGTTACTCTCATATATAGTTTATTTTCCTCGACAATTAAAAAATATATTTCATTAGTGAAGTGGATTCTACTAATATTTTTTTTAATTACTACAAATAAATAAAATAAATAAATTGAATAGGATTTTAAGTTGGAAATGTTTTTTTTTTTTTGTCCTTTTTTTAAAAAAAAAAAAAAAGAAAAAACAGGATAAGAAAAAGAAAAAAAAAATTCTTTAGATACTTGTAAAAAGAATGGTTAAATTTTGCAGCCCCCTCTCCCAAAAGTTTAATCTTTTACATATGGAATTTTAGGTCAACCTTACTTGGAAAGCTATCTTTTTTATTATTTATTTTATTTTGGTTGGAAGTTTTGGTTCAAAATAAGTGATTCCTTACTATTATTTTAAATTTTGAGCAGGATATTTAATTCCAAAGGGTTGGAAAGTAATGCCTTTGTTCAGGAATATTCATCACAATCCTGACTACTTTGTTGACCCTCACAAATTTGATCCTTCAAGATTTGAGGTATATTTGAATTTCCCTATACTTTCTTTTCTTTTTTGTCTTATCATCCCAAAAAGAAAAATTCTTTTCTTTTCTCTATGTTTGATGTTAATGTTACCTACAAAAATATATTCAGCTATGATTTTTAATGAAAAGACAAGCTTTTTTTTTTATGTGAGAACAGAAATTGCAAGTAATATGGCCAACTATACCTATCTTTCACTTTCAACCTCCCAATTTTTTAACTAAAAAAAAAACCTTCCAATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGAAAAGATGTCTAAATCTTTAGATTGAAGTATTTCACAAAGATACTTTTGTTATATCTGTTATTAGAGTTCTATGTAAAAAGAAGATATTTTTATATTTTCAAAAAAAAAAAAAAAAAAAAAAGAAGAAGAAGATATTTTTATACCTTAGACTTAGACTAGCAATTTATGTGCATCTTAAAATATTTGTTAAATATTATCTAACTAAAAAAGGGATCTATCAGGTTGCTCCAAGACCCAATACATTCATGCCATTTGGCGGTGGGGTCCATGCCTGCCCTGGAAATGAGCTTGCCAAGCTTGAAATCTTGATCATGATTCACCATCTAGTCACCAAATTCAGGTCCTTATTTTTCTCTCTTTTTTTTTTTAGAATATAGCATCACAAAGAAATTCAAAATCTTTAATTTATAAAAAATGTGATAACACACTTATTATCATTTTAAAGGTCCATAGTTTGATTTTCTACTTTGAAATTGTTGAAAGAAAATAAATGTATGAAATAATTATAAATATTTTAGTTAATTGAACTATGTTCAAATTGATACGGATTCAGGAAGTCTATGTTTGGTTGCAGGTGGGAATTGGCAGGAGAGAGAGATGGGATTCAGCATGGGCCATTTCCAGTGCCTATGGATGGGCTTCCAATCAGAGTGTGGAGATTATCCACATGA

mRNA sequence

ATGATGCCTCAAAATTTTCTACTCATTTCCATTTTTCCTCTTGCCCTCCTCCTTCTACCTCTAATTTTCTTTCATGTCCAAAAGATCAACAAGAATATTGCAAAGAAGCTCCCTCCTGGCTCAATGGGCTGGCCCCTAATCGGCGAGACCTTTCGTCTCTACTCTCAACATCCATCCTCCTTCTTCCTTGGAAAACAAAAAAGGTATGGGGATATATTCAAGACCCACATTCTTGGGTGTCCGTGTGTGATGTTGGCGACTCCCGAGGCGGCTCGGTTCGTGCTAGTGACGCAGGCTCATCTCTTCAAGCCCACATACCCACAAAGCAAAGAGCGTCTAATCGGACCATCGGCTCTGTTTTTCCACCAAAGCGAAACCCATTTGCGGCTGCGTAAGCTAATCCAAAGCTCCCTCTCACCGGACCCTGTCCGAGCCCTGGTCCCTGACATCCACGCCCTCGCCCTCTCTACATTGCCCGCATGGCTCAATGGCCATCTCATCAACACTTTCCATCAAATGAAGAAGTTCTCTTTTGAAGTCGGAATTTTGGCGATTTTTGGGCATTTGAAGGACGCCAATTACAGAGACGAATTACTGAGAAACTATTCCATAGTGAACAAAGGCTACAATTCTTTTCCAACCAACATTCCCGGAACGCTTTACAAAAAAGCACTTAAAGCGAGAAAGAGGCTGGGAGAGATCATAAGCTGTATAATCTCAGAGAAAAAGGAGAAGAGAAAGGAATTGGTGGAGAAGAATTTGTTGGGTTGTTTGCTAAAATGGAGAGATGAAAATGGAGAAAGTTTAAGTGATGAACAGATTGCTGATAATATAATTGGAGTTCTGTTTGCTGCTCAGGACACCACTGCCAGTGTTCTGACATGGGTTCTCAAATATCTCCATGATAATCAGAAAGTTCTAGAAGCTGTCAAGGCAGAACAAAAGGCAATTGAAGAGTGTATTGATGAGGGGAGCCGACCAATGAGTTGGACTCAGACACGAAACATGTCGTTTACCAACAAGGTGGTGTTGGAGAGCTTGAGAATGGCGAGTATTATATCATTCACTTTTAGAGAAGCTGTGACAGATGTTGAATATAATGGATATTTAATTCCAAAGGGTTGGAAAGTAATGCCTTTGTTCAGGAATATTCATCACAATCCTGACTACTTTGTTGACCCTCACAAATTTGATCCTTCAAGATTTGAGGTTGCTCCAAGACCCAATACATTCATGCCATTTGGCGGTGGGGTCCATGCCTGCCCTGGAAATGAGCTTGCCAAGCTTGAAATCTTGATCATGATTCACCATCTAGTCACCAAATTCAGGTGGGAATTGGCAGGAGAGAGAGATGGGATTCAGCATGGGCCATTTCCAGTGCCTATGGATGGGCTTCCAATCAGAGTGTGGAGATTATCCACATGA

Coding sequence (CDS)

ATGATGCCTCAAAATTTTCTACTCATTTCCATTTTTCCTCTTGCCCTCCTCCTTCTACCTCTAATTTTCTTTCATGTCCAAAAGATCAACAAGAATATTGCAAAGAAGCTCCCTCCTGGCTCAATGGGCTGGCCCCTAATCGGCGAGACCTTTCGTCTCTACTCTCAACATCCATCCTCCTTCTTCCTTGGAAAACAAAAAAGGTATGGGGATATATTCAAGACCCACATTCTTGGGTGTCCGTGTGTGATGTTGGCGACTCCCGAGGCGGCTCGGTTCGTGCTAGTGACGCAGGCTCATCTCTTCAAGCCCACATACCCACAAAGCAAAGAGCGTCTAATCGGACCATCGGCTCTGTTTTTCCACCAAAGCGAAACCCATTTGCGGCTGCGTAAGCTAATCCAAAGCTCCCTCTCACCGGACCCTGTCCGAGCCCTGGTCCCTGACATCCACGCCCTCGCCCTCTCTACATTGCCCGCATGGCTCAATGGCCATCTCATCAACACTTTCCATCAAATGAAGAAGTTCTCTTTTGAAGTCGGAATTTTGGCGATTTTTGGGCATTTGAAGGACGCCAATTACAGAGACGAATTACTGAGAAACTATTCCATAGTGAACAAAGGCTACAATTCTTTTCCAACCAACATTCCCGGAACGCTTTACAAAAAAGCACTTAAAGCGAGAAAGAGGCTGGGAGAGATCATAAGCTGTATAATCTCAGAGAAAAAGGAGAAGAGAAAGGAATTGGTGGAGAAGAATTTGTTGGGTTGTTTGCTAAAATGGAGAGATGAAAATGGAGAAAGTTTAAGTGATGAACAGATTGCTGATAATATAATTGGAGTTCTGTTTGCTGCTCAGGACACCACTGCCAGTGTTCTGACATGGGTTCTCAAATATCTCCATGATAATCAGAAAGTTCTAGAAGCTGTCAAGGCAGAACAAAAGGCAATTGAAGAGTGTATTGATGAGGGGAGCCGACCAATGAGTTGGACTCAGACACGAAACATGTCGTTTACCAACAAGGTGGTGTTGGAGAGCTTGAGAATGGCGAGTATTATATCATTCACTTTTAGAGAAGCTGTGACAGATGTTGAATATAATGGATATTTAATTCCAAAGGGTTGGAAAGTAATGCCTTTGTTCAGGAATATTCATCACAATCCTGACTACTTTGTTGACCCTCACAAATTTGATCCTTCAAGATTTGAGGTTGCTCCAAGACCCAATACATTCATGCCATTTGGCGGTGGGGTCCATGCCTGCCCTGGAAATGAGCTTGCCAAGCTTGAAATCTTGATCATGATTCACCATCTAGTCACCAAATTCAGGTGGGAATTGGCAGGAGAGAGAGATGGGATTCAGCATGGGCCATTTCCAGTGCCTATGGATGGGCTTCCAATCAGAGTGTGGAGATTATCCACATGA

Protein sequence

MMPQNFLLISIFPLALLLLPLIFFHVQKINKNIAKKLPPGSMGWPLIGETFRLYSQHPSSFFLGKQKRYGDIFKTHILGCPCVMLATPEAARFVLVTQAHLFKPTYPQSKERLIGPSALFFHQSETHLRLRKLIQSSLSPDPVRALVPDIHALALSTLPAWLNGHLINTFHQMKKFSFEVGILAIFGHLKDANYRDELLRNYSIVNKGYNSFPTNIPGTLYKKALKARKRLGEIISCIISEKKEKRKELVEKNLLGCLLKWRDENGESLSDEQIADNIIGVLFAAQDTTASVLTWVLKYLHDNQKVLEAVKAEQKAIEECIDEGSRPMSWTQTRNMSFTNKVVLESLRMASIISFTFREAVTDVEYNGYLIPKGWKVMPLFRNIHHNPDYFVDPHKFDPSRFEVAPRPNTFMPFGGGVHACPGNELAKLEILIMIHHLVTKFRWELAGERDGIQHGPFPVPMDGLPIRVWRLST
Homology
[Close]
BLAST of Lag0004287 vs. NCBI nr
Match: XP_038884199.1 (abscisic acid 8'-hydroxylase CYP707A1-like [Benincasa hispida])

HSP 1 Score: 849.0 bits (2192), Expect = 2.0e-242
Identity = 415/469 (88.49%), Postives = 433/469 (92.32%), Query Frame = 0 

Query: 6   FLLISIFPLALLLLPLIFFHVQKINKNIAKKLPPGSMGWPLIGETFRLYSQHPSSFFLGK 65
           FLLIS F L LL   L FF + K +K   KKLPPGSMGWPLIGETF  YSQ P+SFFLGK
Sbjct: 5   FLLISFFLLPLL---LFFFQIHKNSKFPNKKLPPGSMGWPLIGETFHFYSQDPTSFFLGK 64

Query: 66  QKRYGDIFKTHILGCPCVMLATPEAARFVLVTQAHLFKPTYPQSKERLIGPSALFFHQSE 125
           QKRYG+IFKTHI GCPCVMLATPEAARFVLVTQAHLFKPTYPQSKERLIGPSALFFHQSE
Sbjct: 65  QKRYGEIFKTHIHGCPCVMLATPEAARFVLVTQAHLFKPTYPQSKERLIGPSALFFHQSE 124

Query: 126 THLRLRKLIQSSLSPDPVRALVPDIHALALSTLPAWLNGHLINTFHQMKKFSFEVGILAI 185
           THLRLRKLIQSSLSPDPVRALVPDIHALA+S LP+WLNGHLINTFHQMKKFSFEVGILAI
Sbjct: 125 THLRLRKLIQSSLSPDPVRALVPDIHALAISALPSWLNGHLINTFHQMKKFSFEVGILAI 184

Query: 186 FGHLKDANYRDELLRNYSIVNKGYNSFPTNIPGTLYKKALKARKRLGEIISCIISEKKEK 245
           FGHL+D  YR+EL+ NYSIVNKGYNSFPTNIPGTLYKKALKARKRLGEIISCII EK+EK
Sbjct: 185 FGHLEDTKYREELMTNYSIVNKGYNSFPTNIPGTLYKKALKARKRLGEIISCIILEKREK 244

Query: 246 RKELVEKNLLGCLLKWRDENGESLSDEQIADNIIGVLFAAQDTTASVLTWVLKYLHDNQK 305
           RK+L EKNLL CLLKWRDE GE+LSDEQIADNIIGVLFAAQDTTASVLTWV+KYLH N K
Sbjct: 245 RKQLEEKNLLACLLKWRDEKGETLSDEQIADNIIGVLFAAQDTTASVLTWVVKYLHYNHK 304

Query: 306 VLEAVKAEQKAIEECIDEGSRPMSWTQTRNMSFTNKVVLESLRMASIISFTFREAVTDVE 365
           VLEAVK EQKAIEE I+EG RPMSW+QTRNM FTNKVVLES RMASIISFTFREAV DVE
Sbjct: 305 VLEAVKVEQKAIEEGINEGCRPMSWSQTRNMPFTNKVVLESFRMASIISFTFREAVADVE 364

Query: 366 YNGYLIPKGWKVMPLFRNIHHNPDYFVDPHKFDPSRFEVAPRPNTFMPFGGGVHACPGNE 425
           Y GYLIPKGWKVMPLFRNIHHNPDYFVDPHKFDPSRFEVAPRPNTFMPFG GVHACPGNE
Sbjct: 365 YKGYLIPKGWKVMPLFRNIHHNPDYFVDPHKFDPSRFEVAPRPNTFMPFGSGVHACPGNE 424

Query: 426 LAKLEILIMIHHLVTKFRWELAGERDGIQHGPFPVPMDGLPIRVWRLST 475
           LAKLEILIMIHHLVTKFRWELA +RDGIQHGPFPVP++GLPIRVWRLST
Sbjct: 425 LAKLEILIMIHHLVTKFRWELAEQRDGIQHGPFPVPINGLPIRVWRLST 470

[Close]
BLAST of Lag0004287 vs. NCBI nr
Match: XP_022962256.1 (abscisic acid 8'-hydroxylase 3-like [Cucurbita moschata])

HSP 1 Score: 817.8 bits (2111), Expect = 5.0e-233
Identity = 396/472 (83.90%), Postives = 428/472 (90.68%), Query Frame = 0 

Query: 2   MPQNFLLISIFPLALLLLPLIFFHVQKINKNIAKKLPPGSMGWPLIGETFRLYSQHPSSF 61
           M   F+LISIF     LL   F+ + K N+   KKLPPGSMGWPLIGETF  YSQ   +F
Sbjct: 1   MAATFVLISIF---FPLLLCFFWKIHKNNRIPNKKLPPGSMGWPLIGETFHFYSQ-DLTF 60

Query: 62  FLGKQKRYGDIFKTHILGCPCVMLATPEAARFVLVTQAHLFKPTYPQSKERLIGPSALFF 121
           F  KQKRYG+IFKTHI GCPCVML TPEAARFVLVTQAHLFKPTYPQSKERLIGPSALFF
Sbjct: 61  FFRKQKRYGEIFKTHIHGCPCVMLTTPEAARFVLVTQAHLFKPTYPQSKERLIGPSALFF 120

Query: 122 HQSETHLRLRKLIQSSLSPDPVRALVPDIHALALSTLPAWLNGHLINTFHQMKKFSFEVG 181
           HQSETHLRLRKLIQSSLSPDPVR LVP IHALA+STLP+WLNGHLINT HQMKKFSFEVG
Sbjct: 121 HQSETHLRLRKLIQSSLSPDPVRTLVPHIHALAISTLPSWLNGHLINTLHQMKKFSFEVG 180

Query: 182 ILAIFGHLKDANYRDELLRNYSIVNKGYNSFPTNIPGTLYKKALKARKRLGEIISCIISE 241
           IL IFGHL++ANYRDELL+NYSIVNKGYNSFPTN PG++YKKALKAR RLGEII+CIISE
Sbjct: 181 ILVIFGHLENANYRDELLKNYSIVNKGYNSFPTNFPGSIYKKALKARNRLGEIINCIISE 240

Query: 242 KKEKRKELVEKNLLGCLLKWRDENGESLSDEQIADNIIGVLFAAQDTTASVLTWVLKYLH 301
           K+E+RKEL EKNLL CLLKWRDE GESLSD+QIADNIIGVLFAAQDTTASVLTWV+KYLH
Sbjct: 241 KREQRKELEEKNLLACLLKWRDEKGESLSDDQIADNIIGVLFAAQDTTASVLTWVVKYLH 300

Query: 302 DNQKVLEAVKAEQKAIEECIDEGSRPMSWTQTRNMSFTNKVVLESLRMASIISFTFREAV 361
           DN K+LEAVKAEQ+AI+E I+EGSR MSW  TRNM FTNKVVLESLRMA++ISF+FREAV
Sbjct: 301 DNDKILEAVKAEQRAIQEAINEGSRAMSWADTRNMPFTNKVVLESLRMATVISFSFREAV 360

Query: 362 TDVEYNGYLIPKGWKVMPLFRNIHHNPDYFVDPHKFDPSRFEVAPRPNTFMPFGGGVHAC 421
           TDVEYNGYLIPKGWKVMPLFRNIHHNPDYF+ PHKFDPSRFEVAPRPNTF+PFG GVHAC
Sbjct: 361 TDVEYNGYLIPKGWKVMPLFRNIHHNPDYFLHPHKFDPSRFEVAPRPNTFIPFGSGVHAC 420

Query: 422 PGNELAKLEILIMIHHLVTKFRWELAGERDGIQHGPFPVPMDGLPIRVWRLS 474
           PGN+LAKLEILIMIHHLVTKFRWELAG++DGI+HGPFPVP DGLPIR+WRLS
Sbjct: 421 PGNDLAKLEILIMIHHLVTKFRWELAGQKDGIEHGPFPVPRDGLPIRLWRLS 468

[Close]
BLAST of Lag0004287 vs. NCBI nr
Match: XP_023546528.1 (abscisic acid 8'-hydroxylase 3-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 817.4 bits (2110), Expect = 6.5e-233
Identity = 396/473 (83.72%), Postives = 428/473 (90.49%), Query Frame = 0 

Query: 2   MPQNFLLISI-FPLALLLLPLIFFHVQKINKNIAKKLPPGSMGWPLIGETFRLYSQHPSS 61
           M   F+LISI FPL L      F+ + K N+   KKLPPGSMGWPLIGETF  YSQ   +
Sbjct: 4   MAATFVLISILFPLLL----CFFWKIHKNNRIPNKKLPPGSMGWPLIGETFHFYSQ-DLT 63

Query: 62  FFLGKQKRYGDIFKTHILGCPCVMLATPEAARFVLVTQAHLFKPTYPQSKERLIGPSALF 121
           FF  KQKRYG+IFKTHI GCPCVML TPEAARFVLVTQAHLFKPTYPQSKERLIGPSALF
Sbjct: 64  FFFRKQKRYGEIFKTHIHGCPCVMLTTPEAARFVLVTQAHLFKPTYPQSKERLIGPSALF 123

Query: 122 FHQSETHLRLRKLIQSSLSPDPVRALVPDIHALALSTLPAWLNGHLINTFHQMKKFSFEV 181
           FHQSETHLRLRKLIQSSLSPDPVR LVP IHALA+STLP+WLNGHLINT HQMKKFSFEV
Sbjct: 124 FHQSETHLRLRKLIQSSLSPDPVRTLVPHIHALAISTLPSWLNGHLINTLHQMKKFSFEV 183

Query: 182 GILAIFGHLKDANYRDELLRNYSIVNKGYNSFPTNIPGTLYKKALKARKRLGEIISCIIS 241
           GIL IFGHL++ANYRDELL+NYSIVNKGYNSFPTN PG++YKKALKAR RLGEII+CIIS
Sbjct: 184 GILVIFGHLENANYRDELLKNYSIVNKGYNSFPTNFPGSIYKKALKARNRLGEIINCIIS 243

Query: 242 EKKEKRKELVEKNLLGCLLKWRDENGESLSDEQIADNIIGVLFAAQDTTASVLTWVLKYL 301
           EK+E+RKEL EKNLL CLLKWRDE GESLSD+QIADNIIGVLFAAQDTTASVLTWV+KYL
Sbjct: 244 EKREQRKELEEKNLLACLLKWRDEKGESLSDDQIADNIIGVLFAAQDTTASVLTWVVKYL 303

Query: 302 HDNQKVLEAVKAEQKAIEECIDEGSRPMSWTQTRNMSFTNKVVLESLRMASIISFTFREA 361
           HDN K+LEAVK EQ+AI+E I+EGSR MSW  TRNM FTNKVVLESLRMA++ISF+FREA
Sbjct: 304 HDNDKILEAVKVEQRAIQEAINEGSRAMSWADTRNMPFTNKVVLESLRMATVISFSFREA 363

Query: 362 VTDVEYNGYLIPKGWKVMPLFRNIHHNPDYFVDPHKFDPSRFEVAPRPNTFMPFGGGVHA 421
           VTDVEYNGYLIPKGWKVMPLFRNIHHNPDYF+ PHKFDPSRFEVAPRPNTF+PFG GVHA
Sbjct: 364 VTDVEYNGYLIPKGWKVMPLFRNIHHNPDYFLHPHKFDPSRFEVAPRPNTFIPFGSGVHA 423

Query: 422 CPGNELAKLEILIMIHHLVTKFRWELAGERDGIQHGPFPVPMDGLPIRVWRLS 474
           CPGN+LAKLEILIMIHHLVTKFRWELAG++DGI+HGPFPVP DGLPIR+WRLS
Sbjct: 424 CPGNDLAKLEILIMIHHLVTKFRWELAGQKDGIEHGPFPVPRDGLPIRLWRLS 471

[Close]
BLAST of Lag0004287 vs. NCBI nr
Match: XP_022144125.1 (abscisic acid 8'-hydroxylase 4-like [Momordica charantia])

HSP 1 Score: 805.1 bits (2078), Expect = 3.3e-229
Identity = 397/476 (83.40%), Postives = 425/476 (89.29%), Query Frame = 0 

Query: 2   MPQNFLLISIF---PLALLLLPLIFFHVQKINKNIAKKLPPGSMGWPLIGETFRLYSQHP 61
           M + F+L SIF    L LLLLPLIFF   K   N  KKLPPGSMGWPLIGETF LYS+ P
Sbjct: 1   MGEIFVLFSIFLPTLLLLLLLPLIFF---KKENNKGKKLPPGSMGWPLIGETFHLYSRDP 60

Query: 62  SSFFLGKQKRYGDIFKTHILGCPCVMLATPEAARFVLVTQAHLFKPTYPQSKERLIGPSA 121
           + FFL KQKRYG+IFKTHI GCPCVMLATPEAARFVLVTQAHLFKPTYP  KERLIGPSA
Sbjct: 61  TIFFLDKQKRYGEIFKTHIHGCPCVMLATPEAARFVLVTQAHLFKPTYPHGKERLIGPSA 120

Query: 122 LFFHQSETHLRLRKLIQSSLSPDPVRALVPDIHALALSTLPAWLNGHLINTFHQMKKFSF 181
           LFFHQS+ HLRLRKL+QSSLSPDPVRALVP I ALA S L  WL+GH +NTF +MKKFSF
Sbjct: 121 LFFHQSDAHLRLRKLVQSSLSPDPVRALVPYIDALASSALAFWLHGHTVNTFEEMKKFSF 180

Query: 182 EVGILAIFGHLKDANYRDELLRNYSIVNKGYNSFPTNIPGTLYKKALKARKRLGEIISCI 241
           EVGILAIFGHL+D NYRDEL++NYS+VN+GYNSF TNIPGT+YKKALKARKRLGEIISCI
Sbjct: 181 EVGILAIFGHLEDDNYRDELMKNYSMVNRGYNSFLTNIPGTIYKKALKARKRLGEIISCI 240

Query: 242 ISEKKEK-RKELVEKNLLGCLLKWRDENGESLSDEQIADNIIGVLFAAQDTTASVLTWVL 301
           ISEK++   K+L EKNLLGCLLKWRDE GE+LSDEQIADN+IGVLFAAQDTTASVLTWVL
Sbjct: 241 ISEKRQNTTKKLAEKNLLGCLLKWRDEKGETLSDEQIADNLIGVLFAAQDTTASVLTWVL 300

Query: 302 KYLHDNQKVLEAVKAEQKAIEECIDEGSRPMSWTQTRNMSFTNKVVLESLRMASIISFTF 361
           KYLHDN+KVL+AVKAEQKAIEE I+ GSRPMSW+ TRNM FTNKVVLESLRMASII+FTF
Sbjct: 301 KYLHDNRKVLQAVKAEQKAIEEAINGGSRPMSWSHTRNMPFTNKVVLESLRMASIITFTF 360

Query: 362 REAVTDVEYNGYLIPKGWKVMPLFRNIHHNPDYFVDPHKFDPSRFEVAPRPNTFMPFGGG 421
           REAV DVEYNGYLIPKGWKVMPLF NIHHN +YFVDPHKFDPSRFEVAPRPNTFMPFG G
Sbjct: 361 REAVADVEYNGYLIPKGWKVMPLFSNIHHNSEYFVDPHKFDPSRFEVAPRPNTFMPFGSG 420

Query: 422 VHACPGNELAKLEILIMIHHLVTKFRWELAGERDGIQHGPFPVPMDGLPIRVWRLS 474
           VHACPGNELAKLEILIMIHHLVTKFRWE AG  DGI HGPFPVP+DGLPIRVWRLS
Sbjct: 421 VHACPGNELAKLEILIMIHHLVTKFRWEFAGRGDGIHHGPFPVPLDGLPIRVWRLS 473

[Close]
BLAST of Lag0004287 vs. NCBI nr
Match: KAA0060904.1 (abscisic acid 8'-hydroxylase 4-like [Cucumis melo var. makuwa])

HSP 1 Score: 803.1 bits (2073), Expect = 1.3e-228
Identity = 387/434 (89.17%), Postives = 407/434 (93.78%), Query Frame = 0 

Query: 42  MGWPLIGETFRLYSQHPSSFFLGKQKRYGDIFKTHILGCPCVMLATPEAARFVLVTQAHL 101
           MGWPLIGETF  YSQHP+SFFLGKQKRYG+IFKTHI GCPCVMLATPEAARFVLVTQAHL
Sbjct: 1   MGWPLIGETFHFYSQHPTSFFLGKQKRYGEIFKTHIHGCPCVMLATPEAARFVLVTQAHL 60

Query: 102 FKPTYPQSKERLIGPSALFFHQSETHLRLRKLIQSSLSPDPVRALVPDIHALALSTLPAW 161
           FKPTYPQSKERLIGPSALFFHQSETHLRLRKLIQSSLSPDPVRALVP IHALA+S LP+ 
Sbjct: 61  FKPTYPQSKERLIGPSALFFHQSETHLRLRKLIQSSLSPDPVRALVPHIHALAISALPSS 120

Query: 162 LNGHLINTFHQMKKFSFEVGILAIFGHLKDANYRDELLRNYSIVNKGYNSFPTNIPGTLY 221
           L+GH+I+T+HQMKKFSFEVGILAIFGHL+D NYR+EL  NYSIVNKGYNSFPTNIPGTLY
Sbjct: 121 LSGHVIDTYHQMKKFSFEVGILAIFGHLEDTNYREELRTNYSIVNKGYNSFPTNIPGTLY 180

Query: 222 KKALKARKRLGEIISCIISEKKEKR-KELVEKNLLGCLLKWRDENGESLSDEQIADNIIG 281
           KKA+KARKRL EIISCII EKKEKR K L EKNLL CL+KWRDE GESLSDEQIADNIIG
Sbjct: 181 KKAIKARKRLEEIISCIILEKKEKRSKVLGEKNLLSCLVKWRDEKGESLSDEQIADNIIG 240

Query: 282 VLFAAQDTTASVLTWVLKYLHDNQKVLEAVKAEQKAIEECIDEGSRPMSWTQTRNMSFTN 341
           VLFAAQDTTASVLTWV+KYLHDN KVLEAVK EQK IEE I+EGSRPM W+ TRNM FTN
Sbjct: 241 VLFAAQDTTASVLTWVVKYLHDNPKVLEAVKVEQKVIEEDINEGSRPMRWSHTRNMPFTN 300

Query: 342 KVVLESLRMASIISFTFREAVTDVEYNGYLIPKGWKVMPLFRNIHHNPDYFVDPHKFDPS 401
           KVVLESLRMASIISFTFREAV DVEY GYLIPKGWKVMPLFRNIHHNPDYFVDPHKFDPS
Sbjct: 301 KVVLESLRMASIISFTFREAVADVEYKGYLIPKGWKVMPLFRNIHHNPDYFVDPHKFDPS 360

Query: 402 RFEVAPRPNTFMPFGGGVHACPGNELAKLEILIMIHHLVTKFRWELAGERDGIQHGPFPV 461
           RFEVAPRPNTFMPFG GVH+CPGNELAKLEI IMIHHLVTKFRWELAG+RDGI+HGPFPV
Sbjct: 361 RFEVAPRPNTFMPFGSGVHSCPGNELAKLEIFIMIHHLVTKFRWELAGQRDGIEHGPFPV 420

Query: 462 PMDGLPIRVWRLST 475
           P++GLPIRVWRLST
Sbjct: 421 PINGLPIRVWRLST 434

[Close]
BLAST of Lag0004287 vs. ExPASy Swiss-Prot
Match: A9QNE7 (Abscisic acid 8'-hydroxylase CYP707A1 OS=Solanum lycopersicum OX=4081 GN=CYP707A1 PE=2 SV=1)

HSP 1 Score: 644.8 bits (1662), Expect = 7.6e-184
Identity = 313/471 (66.45%), Postives = 382/471 (81.10%), Query Frame = 0 

Query: 6   FLLISIFPLALLLLPLIF-FHVQKINKNIAKKLPPGSMGWPLIGETFRLYSQHPSSFFLG 65
           FL IS+F L  L     F  +    +K  A KLPPGSMGWP IGET +LYSQ P++FF+ 
Sbjct: 8   FLYISMFVLGYLSYYFCFGKNNNSSSKKNAYKLPPGSMGWPYIGETLQLYSQDPNAFFIN 67

Query: 66  KQKRYGDIFKTHILGCPCVMLATPEAARFVLVTQAHLFKPTYPQSKERLIGPSALFFHQS 125
           +Q+R+G+IFKT ILGCPCVMLA+PEAARFVLV QA+LFKPTYP+SKE LIG SA+FFHQ 
Sbjct: 68  RQRRFGEIFKTKILGCPCVMLASPEAARFVLVNQANLFKPTYPKSKENLIGQSAIFFHQG 127

Query: 126 ETHLRLRKLIQSSLSPDPVRALVPDIHALALSTLPAWLNGHLINTFHQMKKFSFEVGILA 185
           + H  LRKL+Q+ L+P+ +R  +P I  L++S L +W+ GH++NT+H+MKKFSFEVGILA
Sbjct: 128 DYHNHLRKLVQAPLNPESIRNQIPYIEELSISALNSWVGGHVVNTYHEMKKFSFEVGILA 187

Query: 186 IFGHLKDANYRDELLRNYSIVNKGYNSFPTNIPGTLYKKALKARKRLGEIISCIISEKKE 245
           IFGHL D + ++EL +NYSIV+KGYNSFP N+PGTLY+KAL+ARK+LG+I+S II E KE
Sbjct: 188 IFGHL-DGHVKEELKKNYSIVDKGYNSFPINLPGTLYRKALQARKKLGKILSEIIREMKE 247

Query: 246 KRKELVEKNLLGCLLKWRDENG-ESLSDEQIADNIIGVLFAAQDTTASVLTWVLKYLHDN 305
           K+   +EK LL C L  ++E G   L+++QIADNIIGVLFAAQDTTASVLTW++KYLHDN
Sbjct: 248 KK--TLEKGLLSCFLNAKEEKGFLVLNEDQIADNIIGVLFAAQDTTASVLTWIIKYLHDN 307

Query: 306 QKVLEAVKAEQKAIEECIDEGSRPMSWTQTRNMSFTNKVVLESLRMASIISFTFREAVTD 365
            K+LE VKAEQK I +  ++ +  ++WTQTR M  T++VVLE+LRMASIISF FREAV D
Sbjct: 308 PKLLECVKAEQKVIWQSNEQENHGLTWTQTRKMPITSRVVLETLRMASIISFAFREAVAD 367

Query: 366 VEYNGYLIPKGWKVMPLFRNIHHNPDYFVDPHKFDPSRFEVAPRPNTFMPFGGGVHACPG 425
           VEY GYLIPKGWKVMPLFRNIHHNP++F DP KFDPSRFE AP+PNTFMPFG GVHACPG
Sbjct: 368 VEYKGYLIPKGWKVMPLFRNIHHNPEFFPDPQKFDPSRFENAPKPNTFMPFGSGVHACPG 427

Query: 426 NELAKLEILIMIHHLVTKFRWELAGERDGIQHGPFPVPMDGLPIRVWRLST 475
           NELAKLEILIM HHLVTKFRWE+ G   GIQ+GPFPVP+ GL  R W+ ++
Sbjct: 428 NELAKLEILIMTHHLVTKFRWEVVGSGSGIQYGPFPVPLGGLAARFWKTTS 475

[Close]
BLAST of Lag0004287 vs. ExPASy Swiss-Prot
Match: Q9LJK2 (Abscisic acid 8'-hydroxylase 4 OS=Arabidopsis thaliana OX=3702 GN=CYP707A4 PE=2 SV=2)

HSP 1 Score: 630.9 bits (1626), Expect = 1.1e-179
Identity = 307/461 (66.59%), Postives = 374/461 (81.13%), Query Frame = 0 

Query: 6   FLLISIFPLALLLLPLIFFHVQKINKNIAKKLPPGSMGWPLIGETFRLYSQHPSSFFLGK 65
           FL++ I  L LLL+ +I   V K  KN   KLPPGSMGWP +GET +LYSQ+P+ FF  K
Sbjct: 6   FLVVPILILCLLLVRVI---VSKKKKNSRGKLPPGSMGWPYLGETLQLYSQNPNVFFTSK 65

Query: 66  QKRYGDIFKTHILGCPCVMLATPEAARFVLVTQAHLFKPTYPQSKERLIGPSALFFHQSE 125
           QKRYG+IFKT ILG PCVMLA+PEAARFVLVT AH+FKPTYP+SKE+LIGPSALFFHQ +
Sbjct: 66  QKRYGEIFKTRILGYPCVMLASPEAARFVLVTHAHMFKPTYPRSKEKLIGPSALFFHQGD 125

Query: 126 THLRLRKLIQSSLSPDPVRALVPDIHALALSTLPAWLNGHLINTFHQMKKFSFEVGILAI 185
            H  +RKL+QSS  P+ +R L+PDI  +ALS+L +W N  +++T+ +MKKF+F+VGILAI
Sbjct: 126 YHSHIRKLVQSSFYPETIRKLIPDIEHIALSSLQSWANMPIVSTYQEMKKFAFDVGILAI 185

Query: 186 FGHLKDANYRDELLRNYSIVNKGYNSFPTNIPGTLYKKALKARKRLGEIISCIISEKKEK 245
           FGHL +++Y++ L  NY+IV+KGYNSFP ++PGT Y KAL ARK+L  I+S II E++EK
Sbjct: 186 FGHL-ESSYKEILKHNYNIVDKGYNSFPMSLPGTSYHKALMARKQLKTIVSEIICERREK 245

Query: 246 RKELVEKNLLGCLLKWRDENGESLSDEQIADNIIGVLFAAQDTTASVLTWVLKYLHDNQK 305
           R   ++ + LG LL +++E G  L+ EQIADNIIGVLFAAQDTTAS LTW+LKYLHD+QK
Sbjct: 246 R--ALQTDFLGHLLNFKNEKGRVLTQEQIADNIIGVLFAAQDTTASCLTWILKYLHDDQK 305

Query: 306 VLEAVKAEQKAIEECIDEGSRPMSWTQTRNMSFTNKVVLESLRMASIISFTFREAVTDVE 365
           +LEAVKAEQKAI E      +P++W QTRNM  T+KV++ESLRMASIISFTFREAV DVE
Sbjct: 306 LLEAVKAEQKAIYEENSREKKPLTWRQTRNMPLTHKVIVESLRMASIISFTFREAVVDVE 365

Query: 366 YNGYLIPKGWKVMPLFRNIHHNPDYFVDPHKFDPSRFEVAPRPNTFMPFGGGVHACPGNE 425
           Y GYLIPKGWKVMPLFRNIHHNP YF +P  FDPSRFEV P+PNTFMPFG GVHACPGNE
Sbjct: 366 YKGYLIPKGWKVMPLFRNIHHNPKYFSNPEVFDPSRFEVNPKPNTFMPFGSGVHACPGNE 425

Query: 426 LAKLEILIMIHHLVTKFRWELAGERDGIQHGPFPVPMDGLP 467
           LAKL+ILI +HHLV+ FRWE+ G   GIQ+ PFP+P +GLP
Sbjct: 426 LAKLQILIFLHHLVSNFRWEVKGGEKGIQYSPFPIPQNGLP 460

[Close]
BLAST of Lag0004287 vs. ExPASy Swiss-Prot
Match: Q0J185 (Abscisic acid 8'-hydroxylase 3 OS=Oryza sativa subsp. japonica OX=39947 GN=CYP707A7 PE=2 SV=1)

HSP 1 Score: 630.2 bits (1624), Expect = 1.9e-179
Identity = 298/439 (67.88%), Postives = 362/439 (82.46%), Query Frame = 0 

Query: 34  AKKLPPGSMGWPLIGETFRLYSQHPSSFFLGKQKRYGDIFKTHILGCPCVMLATPEAARF 93
           A +LPPGSMGWP IGET +LYSQ P+ FF  KQKRYG+IFKTHILGCPCVMLA+PEAARF
Sbjct: 41  AVRLPPGSMGWPYIGETLQLYSQDPNVFFASKQKRYGEIFKTHILGCPCVMLASPEAARF 100

Query: 94  VLVTQAHLFKPTYPQSKERLIGPSALFFHQSETHLRLRKLIQSSLSPDPVRALVPDIHAL 153
           VLVTQAHLFKPTYP+SKER+IGPSALFFHQ + HLRLRKL+Q  L PD +RALVPD+ A 
Sbjct: 101 VLVTQAHLFKPTYPRSKERMIGPSALFFHQGDYHLRLRKLVQGPLGPDALRALVPDVEAA 160

Query: 154 ALSTLPAWLNGHLINTFHQMKKFSFEVGILAIFGHLKDANYRDELLRNYSIVNKGYNSFP 213
             STL +W +G++ +TFH MK+ SF+VGI+ IFG   D   + EL +NY+IV KGYNSFP
Sbjct: 161 VRSTLASW-DGNVSSTFHAMKRLSFDVGIVTIFGGRLDERRKAELRQNYAIVEKGYNSFP 220

Query: 214 TNIPGTLYKKALKARKRLGEIISCIISEKKEKRKELVEKNLLGCLLKWR-DENGESLSDE 273
            + PGTLY KA++AR+RL  ++S I+ E++ + +     +LLGCL++ R  ++G  L+DE
Sbjct: 221 NSFPGTLYYKAIQARRRLHGVLSDIMRERRARGEP--GSDLLGCLMQSRAGDDGALLTDE 280

Query: 274 QIADNIIGVLFAAQDTTASVLTWVLKYLHDNQKVLEAVKAEQKAIEECIDEGSRPMSWTQ 333
           Q+ADNIIGVLFAAQDTTASVLTW++KYLHD+ K+LEAV+AEQ AI    D G  P++W Q
Sbjct: 281 QVADNIIGVLFAAQDTTASVLTWIVKYLHDHPKLLEAVRAEQAAIRAANDGGRLPLTWAQ 340

Query: 334 TRNMSFTNKVVLESLRMASIISFTFREAVTDVEYNGYLIPKGWKVMPLFRNIHHNPDYFV 393
           TR+M+ T+KV+LESLRMASIISFTFREAV DVEY G+LIPKGWKVMPLFRNIHHNPDYF 
Sbjct: 341 TRSMALTHKVILESLRMASIISFTFREAVADVEYKGFLIPKGWKVMPLFRNIHHNPDYFQ 400

Query: 394 DPHKFDPSRFEVAPRPNTFMPFGGGVHACPGNELAKLEILIMIHHLVTKFRWELAGERDG 453
           DP KFDPSRF+V+PRPNTFMPFG GVHACPGNELAKLE+L++IHHLVT +RWE+ G  D 
Sbjct: 401 DPQKFDPSRFKVSPRPNTFMPFGNGVHACPGNELAKLEMLVLIHHLVTGYRWEIVGSSDE 460

Query: 454 IQHGPFPVPMDGLPIRVWR 472
           +++ PFPVP  GL  ++WR
Sbjct: 461 VEYSPFPVPKHGLLAKLWR 476

[Close]
BLAST of Lag0004287 vs. ExPASy Swiss-Prot
Match: A2Z212 (Abscisic acid 8'-hydroxylase 3 OS=Oryza sativa subsp. indica OX=39946 GN=CYP707A7 PE=3 SV=1)

HSP 1 Score: 628.2 bits (1619), Expect = 7.3e-179
Identity = 298/442 (67.42%), Postives = 363/442 (82.13%), Query Frame = 0 

Query: 34  AKKLPPGSMGWPLIGETFRLYSQHPSSFFLGKQKRYGDIFKTHILGCPCVMLATPEAARF 93
           A +LPPGSMGWP IGET +LYSQ P+ FF  KQKRYG+IFKTHILGCPCVMLA+PEAARF
Sbjct: 41  AVRLPPGSMGWPYIGETLQLYSQDPNVFFASKQKRYGEIFKTHILGCPCVMLASPEAARF 100

Query: 94  VLVTQAHLFKPTYPQSKERLIGPSALFFHQSETHLRLRKLIQSSLSPDPVRALVPDIHAL 153
           VLVTQAHLFKPTYP+SKER+IGPSALFF+Q + HLRLRKL+Q  L PD +RALVPD+ A 
Sbjct: 101 VLVTQAHLFKPTYPRSKERMIGPSALFFNQGDYHLRLRKLVQGPLGPDALRALVPDVEAA 160

Query: 154 ALSTLPAWLNGHLINTFHQMKKFSFEVGILAIFGHLKDANYRDELLRNYSIVNKGYNSFP 213
             STL +W +G++ +TFH MK+ SF+VGI+ IFG   D   + EL +NY+IV KGYNSFP
Sbjct: 161 VRSTLASW-DGNVSSTFHAMKRLSFDVGIVTIFGGRLDERRKAELRQNYAIVEKGYNSFP 220

Query: 214 TNIPGTLYKKALKARKRLGEIISCIISEKKEKRKELVEKNLLGCLLKWR-DENGESLSDE 273
            + PGTLY KA++AR+RL  ++S I+ E++ + +     +LLGCL++ R  ++G  L+DE
Sbjct: 221 NSFPGTLYYKAIQARRRLHGVLSDIMRERRARGEP--GSDLLGCLMQSRAGDDGALLTDE 280

Query: 274 QIADNIIGVLFAAQDTTASVLTWVLKYLHDNQKVLEAVKAEQKAIEECIDEGSRPMSWTQ 333
           Q+ADNIIGVLFAAQDTTASVLTW++KYLHD+ K+LEAV+AEQ AI    D G  P++W Q
Sbjct: 281 QVADNIIGVLFAAQDTTASVLTWIVKYLHDHPKLLEAVRAEQAAIRAANDGGRLPLTWAQ 340

Query: 334 TRNMSFTNKVVLESLRMASIISFTFREAVTDVEYNGYLIPKGWKVMPLFRNIHHNPDYFV 393
           TR+M+ T+KV+LESLRMASIISFTFREAV DVEY G+LIPKGWKVMPLFRNIHHNPDYF 
Sbjct: 341 TRSMALTHKVILESLRMASIISFTFREAVADVEYKGFLIPKGWKVMPLFRNIHHNPDYFQ 400

Query: 394 DPHKFDPSRFEVAPRPNTFMPFGGGVHACPGNELAKLEILIMIHHLVTKFRWELAGERDG 453
           DP KFDPSRF+V+PRPNTFMPFG GVHACPGNELAKLE+L++IHHLVT +RWE+ G  D 
Sbjct: 401 DPQKFDPSRFKVSPRPNTFMPFGNGVHACPGNELAKLEMLVLIHHLVTGYRWEIVGSSDE 460

Query: 454 IQHGPFPVPMDGLPIRVWRLST 475
           +++ PFPVP  GL  ++WR  T
Sbjct: 461 VEYSPFPVPKHGLLAKLWRDDT 479

[Close]
BLAST of Lag0004287 vs. ExPASy Swiss-Prot
Match: Q09J78 (Abscisic acid 8'-hydroxylase 2 OS=Oryza sativa subsp. indica OX=39946 GN=CYP707A6 PE=2 SV=1)

HSP 1 Score: 617.8 bits (1592), Expect = 9.9e-176
Identity = 288/440 (65.45%), Postives = 358/440 (81.36%), Query Frame = 0 

Query: 36  KLPPGSMGWPLIGETFRLYSQHPSSFFLGKQKRYGDIFKTHILGCPCVMLATPEAARFVL 95
           +LPPGSMGWP +GET +LYSQ P+ FF  KQKRYG+IFKT++LGCPCVMLA+PEAARFVL
Sbjct: 50  RLPPGSMGWPYVGETLQLYSQDPNVFFASKQKRYGEIFKTNLLGCPCVMLASPEAARFVL 109

Query: 96  VTQAHLFKPTYPQSKERLIGPSALFFHQSETHLRLRKLIQSSLSPDPVRALVPDIHALAL 155
           V+QA LFKPTYP SKER+IGPSALFFHQ E HLRLR+L+Q++L+PD +RALVPD+ A   
Sbjct: 110 VSQARLFKPTYPPSKERMIGPSALFFHQGEYHLRLRRLVQAALAPDSLRALVPDVDAAVA 169

Query: 156 STLPAWLNGHLINTFHQMKKFSFEVGILAIFGHLKDANYRDELLRNYSIVNKGYNSFPTN 215
           +TL AW  GH+ +TFH MKK SF+VG++ IFG      +R+EL  NYS+V +GYN FP  
Sbjct: 170 ATLAAWSGGHVASTFHAMKKLSFDVGVVTIFGGRLGRRHREELRTNYSVVERGYNCFPNR 229

Query: 216 IPGTLYKKALKARKRLGEIISCIISEKKEKRKELVEKNLLGCLLKWRDENGES----LSD 275
            PGTLY KA++ARKRL  I+S I++E++ +       +LLG L++ RD+        L+D
Sbjct: 230 FPGTLYHKAIQARKRLRAILSEIVAERRARGGG--GDDLLGGLMRSRDDGTAGAVALLTD 289

Query: 276 EQIADNIIGVLFAAQDTTASVLTWVLKYLHDNQKVLEAVKAEQKAIEECIDEGSRPMSWT 335
           +QIADN++GVLFAAQDTTASVLTW+LKYLHD+ K+LEAVKAEQ AI    + G RP++WT
Sbjct: 290 DQIADNVVGVLFAAQDTTASVLTWILKYLHDSPKLLEAVKAEQMAIYVANEGGKRPLTWT 349

Query: 336 QTRNMSFTNKVVLESLRMASIISFTFREAVTDVEYNGYLIPKGWKVMPLFRNIHHNPDYF 395
           QTR+M+ T++V+LESLRMASIISFTFREAV DVEY G+LIPKGWKVMPLFRNIHHNPDYF
Sbjct: 350 QTRSMTLTHQVILESLRMASIISFTFREAVADVEYKGFLIPKGWKVMPLFRNIHHNPDYF 409

Query: 396 VDPHKFDPSRFEVAPRPNTFMPFGGGVHACPGNELAKLEILIMIHHLVTKFRWELAGERD 455
            DP KFDPSRF+VAPRP+TF+PFG GVHACPGNELAKLE+L+++H LVT +RWE+ G  D
Sbjct: 410 QDPQKFDPSRFKVAPRPSTFLPFGSGVHACPGNELAKLEMLVLVHRLVTAYRWEIVGASD 469

Query: 456 GIQHGPFPVPMDGLPIRVWR 472
            +++ PFPVP  GL  ++W+
Sbjct: 470 EVEYSPFPVPRGGLNAKLWK 487

[Close]
BLAST of Lag0004287 vs. ExPASy TrEMBL
Match: A0A6J1HCL5 (abscisic acid 8'-hydroxylase 3-like OS=Cucurbita moschata OX=3662 GN=LOC111462760 PE=3 SV=1)

HSP 1 Score: 817.8 bits (2111), Expect = 2.4e-233
Identity = 396/472 (83.90%), Postives = 428/472 (90.68%), Query Frame = 0 

Query: 2   MPQNFLLISIFPLALLLLPLIFFHVQKINKNIAKKLPPGSMGWPLIGETFRLYSQHPSSF 61
           M   F+LISIF     LL   F+ + K N+   KKLPPGSMGWPLIGETF  YSQ   +F
Sbjct: 1   MAATFVLISIF---FPLLLCFFWKIHKNNRIPNKKLPPGSMGWPLIGETFHFYSQ-DLTF 60

Query: 62  FLGKQKRYGDIFKTHILGCPCVMLATPEAARFVLVTQAHLFKPTYPQSKERLIGPSALFF 121
           F  KQKRYG+IFKTHI GCPCVML TPEAARFVLVTQAHLFKPTYPQSKERLIGPSALFF
Sbjct: 61  FFRKQKRYGEIFKTHIHGCPCVMLTTPEAARFVLVTQAHLFKPTYPQSKERLIGPSALFF 120

Query: 122 HQSETHLRLRKLIQSSLSPDPVRALVPDIHALALSTLPAWLNGHLINTFHQMKKFSFEVG 181
           HQSETHLRLRKLIQSSLSPDPVR LVP IHALA+STLP+WLNGHLINT HQMKKFSFEVG
Sbjct: 121 HQSETHLRLRKLIQSSLSPDPVRTLVPHIHALAISTLPSWLNGHLINTLHQMKKFSFEVG 180

Query: 182 ILAIFGHLKDANYRDELLRNYSIVNKGYNSFPTNIPGTLYKKALKARKRLGEIISCIISE 241
           IL IFGHL++ANYRDELL+NYSIVNKGYNSFPTN PG++YKKALKAR RLGEII+CIISE
Sbjct: 181 ILVIFGHLENANYRDELLKNYSIVNKGYNSFPTNFPGSIYKKALKARNRLGEIINCIISE 240

Query: 242 KKEKRKELVEKNLLGCLLKWRDENGESLSDEQIADNIIGVLFAAQDTTASVLTWVLKYLH 301
           K+E+RKEL EKNLL CLLKWRDE GESLSD+QIADNIIGVLFAAQDTTASVLTWV+KYLH
Sbjct: 241 KREQRKELEEKNLLACLLKWRDEKGESLSDDQIADNIIGVLFAAQDTTASVLTWVVKYLH 300

Query: 302 DNQKVLEAVKAEQKAIEECIDEGSRPMSWTQTRNMSFTNKVVLESLRMASIISFTFREAV 361
           DN K+LEAVKAEQ+AI+E I+EGSR MSW  TRNM FTNKVVLESLRMA++ISF+FREAV
Sbjct: 301 DNDKILEAVKAEQRAIQEAINEGSRAMSWADTRNMPFTNKVVLESLRMATVISFSFREAV 360

Query: 362 TDVEYNGYLIPKGWKVMPLFRNIHHNPDYFVDPHKFDPSRFEVAPRPNTFMPFGGGVHAC 421
           TDVEYNGYLIPKGWKVMPLFRNIHHNPDYF+ PHKFDPSRFEVAPRPNTF+PFG GVHAC
Sbjct: 361 TDVEYNGYLIPKGWKVMPLFRNIHHNPDYFLHPHKFDPSRFEVAPRPNTFIPFGSGVHAC 420

Query: 422 PGNELAKLEILIMIHHLVTKFRWELAGERDGIQHGPFPVPMDGLPIRVWRLS 474
           PGN+LAKLEILIMIHHLVTKFRWELAG++DGI+HGPFPVP DGLPIR+WRLS
Sbjct: 421 PGNDLAKLEILIMIHHLVTKFRWELAGQKDGIEHGPFPVPRDGLPIRLWRLS 468

[Close]
BLAST of Lag0004287 vs. ExPASy TrEMBL
Match: A0A6J1CQS3 (abscisic acid 8'-hydroxylase 4-like OS=Momordica charantia OX=3673 GN=LOC111013891 PE=3 SV=1)

HSP 1 Score: 805.1 bits (2078), Expect = 1.6e-229
Identity = 397/476 (83.40%), Postives = 425/476 (89.29%), Query Frame = 0 

Query: 2   MPQNFLLISIF---PLALLLLPLIFFHVQKINKNIAKKLPPGSMGWPLIGETFRLYSQHP 61
           M + F+L SIF    L LLLLPLIFF   K   N  KKLPPGSMGWPLIGETF LYS+ P
Sbjct: 1   MGEIFVLFSIFLPTLLLLLLLPLIFF---KKENNKGKKLPPGSMGWPLIGETFHLYSRDP 60

Query: 62  SSFFLGKQKRYGDIFKTHILGCPCVMLATPEAARFVLVTQAHLFKPTYPQSKERLIGPSA 121
           + FFL KQKRYG+IFKTHI GCPCVMLATPEAARFVLVTQAHLFKPTYP  KERLIGPSA
Sbjct: 61  TIFFLDKQKRYGEIFKTHIHGCPCVMLATPEAARFVLVTQAHLFKPTYPHGKERLIGPSA 120

Query: 122 LFFHQSETHLRLRKLIQSSLSPDPVRALVPDIHALALSTLPAWLNGHLINTFHQMKKFSF 181
           LFFHQS+ HLRLRKL+QSSLSPDPVRALVP I ALA S L  WL+GH +NTF +MKKFSF
Sbjct: 121 LFFHQSDAHLRLRKLVQSSLSPDPVRALVPYIDALASSALAFWLHGHTVNTFEEMKKFSF 180

Query: 182 EVGILAIFGHLKDANYRDELLRNYSIVNKGYNSFPTNIPGTLYKKALKARKRLGEIISCI 241
           EVGILAIFGHL+D NYRDEL++NYS+VN+GYNSF TNIPGT+YKKALKARKRLGEIISCI
Sbjct: 181 EVGILAIFGHLEDDNYRDELMKNYSMVNRGYNSFLTNIPGTIYKKALKARKRLGEIISCI 240

Query: 242 ISEKKEK-RKELVEKNLLGCLLKWRDENGESLSDEQIADNIIGVLFAAQDTTASVLTWVL 301
           ISEK++   K+L EKNLLGCLLKWRDE GE+LSDEQIADN+IGVLFAAQDTTASVLTWVL
Sbjct: 241 ISEKRQNTTKKLAEKNLLGCLLKWRDEKGETLSDEQIADNLIGVLFAAQDTTASVLTWVL 300

Query: 302 KYLHDNQKVLEAVKAEQKAIEECIDEGSRPMSWTQTRNMSFTNKVVLESLRMASIISFTF 361
           KYLHDN+KVL+AVKAEQKAIEE I+ GSRPMSW+ TRNM FTNKVVLESLRMASII+FTF
Sbjct: 301 KYLHDNRKVLQAVKAEQKAIEEAINGGSRPMSWSHTRNMPFTNKVVLESLRMASIITFTF 360

Query: 362 REAVTDVEYNGYLIPKGWKVMPLFRNIHHNPDYFVDPHKFDPSRFEVAPRPNTFMPFGGG 421
           REAV DVEYNGYLIPKGWKVMPLF NIHHN +YFVDPHKFDPSRFEVAPRPNTFMPFG G
Sbjct: 361 REAVADVEYNGYLIPKGWKVMPLFSNIHHNSEYFVDPHKFDPSRFEVAPRPNTFMPFGSG 420

Query: 422 VHACPGNELAKLEILIMIHHLVTKFRWELAGERDGIQHGPFPVPMDGLPIRVWRLS 474
           VHACPGNELAKLEILIMIHHLVTKFRWE AG  DGI HGPFPVP+DGLPIRVWRLS
Sbjct: 421 VHACPGNELAKLEILIMIHHLVTKFRWEFAGRGDGIHHGPFPVPLDGLPIRVWRLS 473

[Close]
BLAST of Lag0004287 vs. ExPASy TrEMBL
Match: A0A5A7V0Q4 (Abscisic acid 8'-hydroxylase 4-like OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold501G00370 PE=3 SV=1)

HSP 1 Score: 803.1 bits (2073), Expect = 6.1e-229
Identity = 387/434 (89.17%), Postives = 407/434 (93.78%), Query Frame = 0 

Query: 42  MGWPLIGETFRLYSQHPSSFFLGKQKRYGDIFKTHILGCPCVMLATPEAARFVLVTQAHL 101
           MGWPLIGETF  YSQHP+SFFLGKQKRYG+IFKTHI GCPCVMLATPEAARFVLVTQAHL
Sbjct: 1   MGWPLIGETFHFYSQHPTSFFLGKQKRYGEIFKTHIHGCPCVMLATPEAARFVLVTQAHL 60

Query: 102 FKPTYPQSKERLIGPSALFFHQSETHLRLRKLIQSSLSPDPVRALVPDIHALALSTLPAW 161
           FKPTYPQSKERLIGPSALFFHQSETHLRLRKLIQSSLSPDPVRALVP IHALA+S LP+ 
Sbjct: 61  FKPTYPQSKERLIGPSALFFHQSETHLRLRKLIQSSLSPDPVRALVPHIHALAISALPSS 120

Query: 162 LNGHLINTFHQMKKFSFEVGILAIFGHLKDANYRDELLRNYSIVNKGYNSFPTNIPGTLY 221
           L+GH+I+T+HQMKKFSFEVGILAIFGHL+D NYR+EL  NYSIVNKGYNSFPTNIPGTLY
Sbjct: 121 LSGHVIDTYHQMKKFSFEVGILAIFGHLEDTNYREELRTNYSIVNKGYNSFPTNIPGTLY 180

Query: 222 KKALKARKRLGEIISCIISEKKEKR-KELVEKNLLGCLLKWRDENGESLSDEQIADNIIG 281
           KKA+KARKRL EIISCII EKKEKR K L EKNLL CL+KWRDE GESLSDEQIADNIIG
Sbjct: 181 KKAIKARKRLEEIISCIILEKKEKRSKVLGEKNLLSCLVKWRDEKGESLSDEQIADNIIG 240

Query: 282 VLFAAQDTTASVLTWVLKYLHDNQKVLEAVKAEQKAIEECIDEGSRPMSWTQTRNMSFTN 341
           VLFAAQDTTASVLTWV+KYLHDN KVLEAVK EQK IEE I+EGSRPM W+ TRNM FTN
Sbjct: 241 VLFAAQDTTASVLTWVVKYLHDNPKVLEAVKVEQKVIEEDINEGSRPMRWSHTRNMPFTN 300

Query: 342 KVVLESLRMASIISFTFREAVTDVEYNGYLIPKGWKVMPLFRNIHHNPDYFVDPHKFDPS 401
           KVVLESLRMASIISFTFREAV DVEY GYLIPKGWKVMPLFRNIHHNPDYFVDPHKFDPS
Sbjct: 301 KVVLESLRMASIISFTFREAVADVEYKGYLIPKGWKVMPLFRNIHHNPDYFVDPHKFDPS 360

Query: 402 RFEVAPRPNTFMPFGGGVHACPGNELAKLEILIMIHHLVTKFRWELAGERDGIQHGPFPV 461
           RFEVAPRPNTFMPFG GVH+CPGNELAKLEI IMIHHLVTKFRWELAG+RDGI+HGPFPV
Sbjct: 361 RFEVAPRPNTFMPFGSGVHSCPGNELAKLEIFIMIHHLVTKFRWELAGQRDGIEHGPFPV 420

Query: 462 PMDGLPIRVWRLST 475
           P++GLPIRVWRLST
Sbjct: 421 PINGLPIRVWRLST 434

[Close]
BLAST of Lag0004287 vs. ExPASy TrEMBL
Match: A0A0A0LKV6 (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_2G357280 PE=3 SV=1)

HSP 1 Score: 797.3 bits (2058), Expect = 3.4e-227
Identity = 385/436 (88.30%), Postives = 406/436 (93.12%), Query Frame = 0 

Query: 42  MGWPLIGETFRLYSQHPSSFFLGKQKRYGDIFKTHILGCPCVMLATPEAARFVLVTQAHL 101
           MGWPLIGETF  YSQHP+SFFLGKQKRYG+IFKTHI GCPCVMLATPEAARF+LVTQAHL
Sbjct: 1   MGWPLIGETFHFYSQHPTSFFLGKQKRYGEIFKTHIHGCPCVMLATPEAARFILVTQAHL 60

Query: 102 FKPTYPQSKERLIGPSALFFHQSETHLRLRKLIQSSLSPDPVRALVPDIHALALSTLPAW 161
           FKPTYPQSKERLIGPSALFFHQSETHLRLRKLIQSSLSPDPVRALVP IHALA+S LP+ 
Sbjct: 61  FKPTYPQSKERLIGPSALFFHQSETHLRLRKLIQSSLSPDPVRALVPHIHALAISALPSS 120

Query: 162 L-NGHLINTFHQMKKFSFEVGILAIFGHLKDANYRDELLRNYSIVNKGYNSFPTNIPGTL 221
           L NGHLI+T+HQMKKFSFEVGILAIFGHL++ NYR+EL  NYSIVNKGYNSFPTNIPGTL
Sbjct: 121 LNNGHLIDTYHQMKKFSFEVGILAIFGHLENTNYREELRTNYSIVNKGYNSFPTNIPGTL 180

Query: 222 YKKALKARKRLGEIISCIISEKKEKRKELV--EKNLLGCLLKWRDENGESLSDEQIADNI 281
           YKKA+KARKRL EIISCIISEKKEKR E+V  EKNLL CL+KWRDE GESLSDEQIADNI
Sbjct: 181 YKKAIKARKRLEEIISCIISEKKEKRSEVVLGEKNLLSCLVKWRDEKGESLSDEQIADNI 240

Query: 282 IGVLFAAQDTTASVLTWVLKYLHDNQKVLEAVKAEQKAIEECIDEGSRPMSWTQTRNMSF 341
           IGVLFAAQDTTASVLTWV+KYLHDN K+LEAVK EQK IEE I+EGS PM W  TR M F
Sbjct: 241 IGVLFAAQDTTASVLTWVVKYLHDNPKILEAVKVEQKVIEEDINEGSGPMRWAHTRTMPF 300

Query: 342 TNKVVLESLRMASIISFTFREAVTDVEYNGYLIPKGWKVMPLFRNIHHNPDYFVDPHKFD 401
           TNKVVLESLR+ASIISFTFREAV DVEY GYLIPKGWKVMPLFRNIHHNPDYFVDPHKFD
Sbjct: 301 TNKVVLESLRLASIISFTFREAVADVEYKGYLIPKGWKVMPLFRNIHHNPDYFVDPHKFD 360

Query: 402 PSRFEVAPRPNTFMPFGGGVHACPGNELAKLEILIMIHHLVTKFRWELAGERDGIQHGPF 461
           PSRFEVAPRPNTFMPFG GVHACPGNELAKLEILIMIHHLVT FRWELAG+RDGI+HGPF
Sbjct: 361 PSRFEVAPRPNTFMPFGSGVHACPGNELAKLEILIMIHHLVTNFRWELAGQRDGIEHGPF 420

Query: 462 PVPMDGLPIRVWRLST 475
           PVP++GLPIRVWRLST
Sbjct: 421 PVPINGLPIRVWRLST 436

[Close]
BLAST of Lag0004287 vs. ExPASy TrEMBL
Match: A0A6J1K9Y7 (abscisic acid 8'-hydroxylase 3-like OS=Cucurbita maxima OX=3661 GN=LOC111492434 PE=3 SV=1)

HSP 1 Score: 789.6 bits (2038), Expect = 7.0e-225
Identity = 375/432 (86.81%), Postives = 402/432 (93.06%), Query Frame = 0 

Query: 42  MGWPLIGETFRLYSQHPSSFFLGKQKRYGDIFKTHILGCPCVMLATPEAARFVLVTQAHL 101
           MGWPLIGETF  YSQ   +FF  KQKRYG+IFKTHI GCPCVML TPEAARFVLVTQAHL
Sbjct: 1   MGWPLIGETFHFYSQ-DLTFFFRKQKRYGEIFKTHIHGCPCVMLTTPEAARFVLVTQAHL 60

Query: 102 FKPTYPQSKERLIGPSALFFHQSETHLRLRKLIQSSLSPDPVRALVPDIHALALSTLPAW 161
           FKPTYPQSKERLIGPSALFFHQSETHLRLRKLIQSSLSPDPVR LVP IHALA+STLP+W
Sbjct: 61  FKPTYPQSKERLIGPSALFFHQSETHLRLRKLIQSSLSPDPVRTLVPHIHALAISTLPSW 120

Query: 162 LNGHLINTFHQMKKFSFEVGILAIFGHLKDANYRDELLRNYSIVNKGYNSFPTNIPGTLY 221
           LNGHLINT HQMKKFSFEVGIL IFGHL++ANYRDELL+NYSIVNKGYNSFPTN PG++Y
Sbjct: 121 LNGHLINTLHQMKKFSFEVGILVIFGHLENANYRDELLKNYSIVNKGYNSFPTNFPGSIY 180

Query: 222 KKALKARKRLGEIISCIISEKKEKRKELVEKNLLGCLLKWRDENGESLSDEQIADNIIGV 281
           KKALKAR RLGEII+CIISEK+E+RKEL EKNLL CLLKWRD  GESLSD+QIADNIIGV
Sbjct: 181 KKALKARNRLGEIINCIISEKREQRKELEEKNLLACLLKWRDAKGESLSDDQIADNIIGV 240

Query: 282 LFAAQDTTASVLTWVLKYLHDNQKVLEAVKAEQKAIEECIDEGSRPMSWTQTRNMSFTNK 341
           LFAAQDTTASVLTWV+KYLHDN K+LEAVK EQ+AIEE I+EGSR MSW  TRNM FTNK
Sbjct: 241 LFAAQDTTASVLTWVVKYLHDNDKILEAVKVEQRAIEEAINEGSRAMSWADTRNMPFTNK 300

Query: 342 VVLESLRMASIISFTFREAVTDVEYNGYLIPKGWKVMPLFRNIHHNPDYFVDPHKFDPSR 401
           VVLESLRMA++ISF+FREAVTDVEYNGYLIPKGWKVMPLFRNIHHNPDYF+ PHKFDPSR
Sbjct: 301 VVLESLRMATVISFSFREAVTDVEYNGYLIPKGWKVMPLFRNIHHNPDYFLHPHKFDPSR 360

Query: 402 FEVAPRPNTFMPFGGGVHACPGNELAKLEILIMIHHLVTKFRWELAGERDGIQHGPFPVP 461
           FEVAPRPNTF+PFG GVHACPGN+LAKLEILIMIHHLVTKFRWELAG++DGI+HGPFPVP
Sbjct: 361 FEVAPRPNTFIPFGSGVHACPGNDLAKLEILIMIHHLVTKFRWELAGQKDGIEHGPFPVP 420

Query: 462 MDGLPIRVWRLS 474
            DGLPI +WRLS
Sbjct: 421 RDGLPIILWRLS 431

[Close]
BLAST of Lag0004287 vs. TAIR 10
Match: AT3G19270.1 (cytochrome P450, family 707, subfamily A, polypeptide 4 )

HSP 1 Score: 630.9 bits (1626), Expect = 8.0e-181
Identity = 307/461 (66.59%), Postives = 374/461 (81.13%), Query Frame = 0 

Query: 6   FLLISIFPLALLLLPLIFFHVQKINKNIAKKLPPGSMGWPLIGETFRLYSQHPSSFFLGK 65
           FL++ I  L LLL+ +I   V K  KN   KLPPGSMGWP +GET +LYSQ+P+ FF  K
Sbjct: 6   FLVVPILILCLLLVRVI---VSKKKKNSRGKLPPGSMGWPYLGETLQLYSQNPNVFFTSK 65

Query: 66  QKRYGDIFKTHILGCPCVMLATPEAARFVLVTQAHLFKPTYPQSKERLIGPSALFFHQSE 125
           QKRYG+IFKT ILG PCVMLA+PEAARFVLVT AH+FKPTYP+SKE+LIGPSALFFHQ +
Sbjct: 66  QKRYGEIFKTRILGYPCVMLASPEAARFVLVTHAHMFKPTYPRSKEKLIGPSALFFHQGD 125

Query: 126 THLRLRKLIQSSLSPDPVRALVPDIHALALSTLPAWLNGHLINTFHQMKKFSFEVGILAI 185
            H  +RKL+QSS  P+ +R L+PDI  +ALS+L +W N  +++T+ +MKKF+F+VGILAI
Sbjct: 126 YHSHIRKLVQSSFYPETIRKLIPDIEHIALSSLQSWANMPIVSTYQEMKKFAFDVGILAI 185

Query: 186 FGHLKDANYRDELLRNYSIVNKGYNSFPTNIPGTLYKKALKARKRLGEIISCIISEKKEK 245
           FGHL +++Y++ L  NY+IV+KGYNSFP ++PGT Y KAL ARK+L  I+S II E++EK
Sbjct: 186 FGHL-ESSYKEILKHNYNIVDKGYNSFPMSLPGTSYHKALMARKQLKTIVSEIICERREK 245

Query: 246 RKELVEKNLLGCLLKWRDENGESLSDEQIADNIIGVLFAAQDTTASVLTWVLKYLHDNQK 305
           R   ++ + LG LL +++E G  L+ EQIADNIIGVLFAAQDTTAS LTW+LKYLHD+QK
Sbjct: 246 R--ALQTDFLGHLLNFKNEKGRVLTQEQIADNIIGVLFAAQDTTASCLTWILKYLHDDQK 305

Query: 306 VLEAVKAEQKAIEECIDEGSRPMSWTQTRNMSFTNKVVLESLRMASIISFTFREAVTDVE 365
           +LEAVKAEQKAI E      +P++W QTRNM  T+KV++ESLRMASIISFTFREAV DVE
Sbjct: 306 LLEAVKAEQKAIYEENSREKKPLTWRQTRNMPLTHKVIVESLRMASIISFTFREAVVDVE 365

Query: 366 YNGYLIPKGWKVMPLFRNIHHNPDYFVDPHKFDPSRFEVAPRPNTFMPFGGGVHACPGNE 425
           Y GYLIPKGWKVMPLFRNIHHNP YF +P  FDPSRFEV P+PNTFMPFG GVHACPGNE
Sbjct: 366 YKGYLIPKGWKVMPLFRNIHHNPKYFSNPEVFDPSRFEVNPKPNTFMPFGSGVHACPGNE 425

Query: 426 LAKLEILIMIHHLVTKFRWELAGERDGIQHGPFPVPMDGLP 467
           LAKL+ILI +HHLV+ FRWE+ G   GIQ+ PFP+P +GLP
Sbjct: 426 LAKLQILIFLHHLVSNFRWEVKGGEKGIQYSPFPIPQNGLP 460

[Close]
BLAST of Lag0004287 vs. TAIR 10
Match: AT2G29090.1 (cytochrome P450, family 707, subfamily A, polypeptide 2 )

HSP 1 Score: 541.2 bits (1393), Expect = 8.4e-154
Identity = 254/434 (58.53%), Postives = 328/434 (75.58%), Query Frame = 0 

Query: 36  KLPPGSMGWPLIGETFRLYSQHPSSFFLGKQKRYGDIFKTHILGCPCVMLATPEAARFVL 95
           +LPPGSMG P IGET RLY+++P+SFF  +Q +YGDIFKTHILGCPCVM+++PEAAR VL
Sbjct: 48  RLPPGSMGLPYIGETLRLYTENPNSFFATRQNKYGDIFKTHILGCPCVMISSPEAARMVL 107

Query: 96  VTQAHLFKPTYPQSKERLIGPSALFFHQSETHLRLRKLIQSSLSPDPVRALVPDIHALAL 155
           V++AHLFKPTYP SKER+IGP ALFFHQ   H  L++L+QSS  P  +R  V  I  L L
Sbjct: 108 VSKAHLFKPTYPPSKERMIGPEALFFHQGPYHSTLKRLVQSSFMPSALRPTVSHIELLVL 167

Query: 156 STLPAWLNGHLINTFHQMKKFSFEVGILAIFGHLKDANYRDELLRNYSIVNKGYNSFPTN 215
            TL +W +   INT   MK+++F+V I++ FG  ++    D +   Y  + +GYNS P +
Sbjct: 168 QTLSSWTSQKSINTLEYMKRYAFDVAIMSAFGDKEEPTTIDVIKLLYQRLERGYNSMPLD 227

Query: 216 IPGTLYKKALKARKRLGEIISCIISEKKEKRKELVEKNLLGCLLKWRDENGESLSDEQIA 275
           +PGTL+ K++KAR  L E +  +I +++E  +E  E  LLG LL  +D+    LSD QIA
Sbjct: 228 LPGTLFHKSMKARIELSEELRKVIEKRRENGRE--EGGLLGVLLGAKDQKRNGLSDSQIA 287

Query: 276 DNIIGVLFAAQDTTASVLTWVLKYLHDNQKVLEAVKAEQKAIEECIDEGSRPMSWTQTRN 335
           DNIIGV+FAA DTTASVLTW+LKYLHD+  +L+ V  EQ +I + I + +R +SW  TR 
Sbjct: 288 DNIIGVIFAATDTTASVLTWLLKYLHDHPNLLQEVSREQFSIRQKIKKENRRISWEDTRK 347

Query: 336 MSFTNKVVLESLRMASIISFTFREAVTDVEYNGYLIPKGWKVMPLFRNIHHNPDYFVDPH 395
           M  T +V+ E+LR AS++SFTFREAV DVEY+GYLIPKGWKV+PLFR IHH+ ++F DP 
Sbjct: 348 MPLTTRVIQETLRAASVLSFTFREAVQDVEYDGYLIPKGWKVLPLFRRIHHSSEFFPDPE 407

Query: 396 KFDPSRFEVAPRPNTFMPFGGGVHACPGNELAKLEILIMIHHLVTKFRWELAGERDGIQH 455
           KFDPSRFEVAP+P T+MPFG GVH+CPG+ELAKLE+LI++HHL T FRWE+ G+ +GIQ+
Sbjct: 408 KFDPSRFEVAPKPYTYMPFGNGVHSCPGSELAKLEMLILLHHLTTSFRWEVIGDEEGIQY 467

Query: 456 GPFPVPMDGLPIRV 470
           GPFPVP  GLPIRV
Sbjct: 468 GPFPVPKKGLPIRV 479

[Close]
BLAST of Lag0004287 vs. TAIR 10
Match: AT2G29090.2 (cytochrome P450, family 707, subfamily A, polypeptide 2 )

HSP 1 Score: 541.2 bits (1393), Expect = 8.4e-154
Identity = 254/434 (58.53%), Postives = 328/434 (75.58%), Query Frame = 0 

Query: 36  KLPPGSMGWPLIGETFRLYSQHPSSFFLGKQKRYGDIFKTHILGCPCVMLATPEAARFVL 95
           +LPPGSMG P IGET RLY+++P+SFF  +Q +YGDIFKTHILGCPCVM+++PEAAR VL
Sbjct: 48  RLPPGSMGLPYIGETLRLYTENPNSFFATRQNKYGDIFKTHILGCPCVMISSPEAARMVL 107

Query: 96  VTQAHLFKPTYPQSKERLIGPSALFFHQSETHLRLRKLIQSSLSPDPVRALVPDIHALAL 155
           V++AHLFKPTYP SKER+IGP ALFFHQ   H  L++L+QSS  P  +R  V  I  L L
Sbjct: 108 VSKAHLFKPTYPPSKERMIGPEALFFHQGPYHSTLKRLVQSSFMPSALRPTVSHIELLVL 167

Query: 156 STLPAWLNGHLINTFHQMKKFSFEVGILAIFGHLKDANYRDELLRNYSIVNKGYNSFPTN 215
            TL +W +   INT   MK+++F+V I++ FG  ++    D +   Y  + +GYNS P +
Sbjct: 168 QTLSSWTSQKSINTLEYMKRYAFDVAIMSAFGDKEEPTTIDVIKLLYQRLERGYNSMPLD 227

Query: 216 IPGTLYKKALKARKRLGEIISCIISEKKEKRKELVEKNLLGCLLKWRDENGESLSDEQIA 275
           +PGTL+ K++KAR  L E +  +I +++E  +E  E  LLG LL  +D+    LSD QIA
Sbjct: 228 LPGTLFHKSMKARIELSEELRKVIEKRRENGRE--EGGLLGVLLGAKDQKRNGLSDSQIA 287

Query: 276 DNIIGVLFAAQDTTASVLTWVLKYLHDNQKVLEAVKAEQKAIEECIDEGSRPMSWTQTRN 335
           DNIIGV+FAA DTTASVLTW+LKYLHD+  +L+ V  EQ +I + I + +R +SW  TR 
Sbjct: 288 DNIIGVIFAATDTTASVLTWLLKYLHDHPNLLQEVSREQFSIRQKIKKENRRISWEDTRK 347

Query: 336 MSFTNKVVLESLRMASIISFTFREAVTDVEYNGYLIPKGWKVMPLFRNIHHNPDYFVDPH 395
           M  T +V+ E+LR AS++SFTFREAV DVEY+GYLIPKGWKV+PLFR IHH+ ++F DP 
Sbjct: 348 MPLTTRVIQETLRAASVLSFTFREAVQDVEYDGYLIPKGWKVLPLFRRIHHSSEFFPDPE 407

Query: 396 KFDPSRFEVAPRPNTFMPFGGGVHACPGNELAKLEILIMIHHLVTKFRWELAGERDGIQH 455
           KFDPSRFEVAP+P T+MPFG GVH+CPG+ELAKLE+LI++HHL T FRWE+ G+ +GIQ+
Sbjct: 408 KFDPSRFEVAPKPYTYMPFGNGVHSCPGSELAKLEMLILLHHLTTSFRWEVIGDEEGIQY 467

Query: 456 GPFPVPMDGLPIRV 470
           GPFPVP  GLPIRV
Sbjct: 468 GPFPVPKKGLPIRV 479

[Close]
BLAST of Lag0004287 vs. TAIR 10
Match: AT4G19230.1 (cytochrome P450, family 707, subfamily A, polypeptide 1 )

HSP 1 Score: 541.2 bits (1393), Expect = 8.4e-154
Identity = 260/461 (56.40%), Postives = 348/461 (75.49%), Query Frame = 0 

Query: 7   LLISIFPLALLLLPLIFFHVQKINKNIAKKLPPGSMGWPLIGETFRLYSQHPSSFFLGKQ 66
           L +++F  +L L  L     Q+   +    LPPG+MGWP +GETF+LYSQ P+ FF  KQ
Sbjct: 6   LFLTLFAGSLFLYFLRCLISQRRFGSSKLPLPPGTMGWPYVGETFQLYSQDPNVFFQSKQ 65

Query: 67  KRYGDIFKTHILGCPCVMLATPEAARFVLVTQAHLFKPTYPQSKERLIGPSALFFHQSET 126
           KRYG +FKTH+LGCPCVM+++PEAA+FVLVT++HLFKPT+P SKER++G  A+FFHQ + 
Sbjct: 66  KRYGSVFKTHVLGCPCVMISSPEAAKFVLVTKSHLFKPTFPASKERMLGKQAIFFHQGDY 125

Query: 127 HLRLRKLIQSSLSPDPVRALVPDIHALALSTLPAWLNGHLINTFHQMKKFSFEVGILAIF 186
           H +LRKL+  +  P+ +R +VPDI ++A  +L +W  G +INT+ +MK ++F V +L+IF
Sbjct: 126 HAKLRKLVLRAFMPESIRNMVPDIESIAQDSLRSW-EGTMINTYQEMKTYTFNVALLSIF 185

Query: 187 GHLKDANYRDELLRNYSIVNKGYNSFPTNIPGTLYKKALKARKRLGEIISCIISEKKEKR 246
           G   +  YR++L R Y I+ KGYNS P N+PGTL+ K++KARK L +I++ I+SE+++  
Sbjct: 186 GK-DEVLYREDLKRCYYILEKGYNSMPVNLPGTLFHKSMKARKELSQILARILSERRQNG 245

Query: 247 KELVEKNLLGCLLKWRDENGESLSDEQIADNIIGVLFAAQDTTASVLTWVLKYLHDNQKV 306
                 +LLG  +  ++E    L+DEQIADNIIGV+FAA+DTTASV++W+LKYL +N  V
Sbjct: 246 SS--HNDLLGSFMGDKEE----LTDEQIADNIIGVIFAARDTTASVMSWILKYLAENPNV 305

Query: 307 LEAVKAEQKAIEECIDEGSRPMSWTQTRNMSFTNKVVLESLRMASIISFTFREAVTDVEY 366
           LEAV  EQ AI +  +EG   ++W  T+ M  T++V+ E+LR+ASI+SFTFREAV DVEY
Sbjct: 306 LEAVTEEQMAIRKDKEEG-ESLTWGDTKKMPLTSRVIQETLRVASILSFTFREAVEDVEY 365

Query: 367 NGYLIPKGWKVMPLFRNIHHNPDYFVDPHKFDPSRFEVAPRPNTFMPFGGGVHACPGNEL 426
            GYLIPKGWKV+PLFRNIHH+ D F +P KFDPSRFEVAP+PNTFMPFG G H+CPGNEL
Sbjct: 366 EGYLIPKGWKVLPLFRNIHHSADIFSNPGKFDPSRFEVAPKPNTFMPFGNGTHSCPGNEL 425

Query: 427 AKLEILIMIHHLVTKFRWELAGERDGIQHGPFPVPMDGLPI 468
           AKLE+ IMIHHL TK+ W + G  DGIQ+GPF +P +GLPI
Sbjct: 426 AKLEMSIMIHHLTTKYSWSIVGASDGIQYGPFALPQNGLPI 457

[Close]
BLAST of Lag0004287 vs. TAIR 10
Match: AT5G45340.1 (cytochrome P450, family 707, subfamily A, polypeptide 3 )

HSP 1 Score: 540.8 bits (1392), Expect = 1.1e-153
Identity = 260/465 (55.91%), Postives = 346/465 (74.41%), Query Frame = 0 

Query: 7   LLISIFPLALLLLPLIFFHVQKINKNIAKKLPPGSMGWPLIGETFRLYSQHPSSFFLGKQ 66
           L +++   AL L  L F    + + +    LPPG+MG+P +GETF+LYSQ P+ FF  KQ
Sbjct: 6   LFLTLSAAALFLCLLRFIAGVRRSSSTKLPLPPGTMGYPYVGETFQLYSQDPNVFFAAKQ 65

Query: 67  KRYGDIFKTHILGCPCVMLATPEAARFVLVTQAHLFKPTYPQSKERLIGPSALFFHQSET 126
           +RYG +FKTH+LGCPCVM+++PEAA+FVLVT++HLFKPT+P SKER++G  A+FFHQ + 
Sbjct: 66  RRYGSVFKTHVLGCPCVMISSPEAAKFVLVTKSHLFKPTFPASKERMLGKQAIFFHQGDY 125

Query: 127 HLRLRKLIQSSLSPDPVRALVPDIHALALSTLPAWLNGHLINTFHQMKKFSFEVGILAIF 186
           H +LRKL+  +  PD +R +VP I ++A  +L +W +G  +NT+ +MK ++F V +++I 
Sbjct: 126 HSKLRKLVLRAFMPDAIRNMVPHIESIAQESLNSW-DGTQLNTYQEMKTYTFNVALISIL 185

Query: 187 GHLKDANYRDELLRNYSIVNKGYNSFPTNIPGTLYKKALKARKRLGEIISCIISEKKEKR 246
           G   +  YR++L R Y I+ KGYNS P N+PGTL+ KA+KARK L +I++ I+S++++  
Sbjct: 186 GK-DEVYYREDLKRCYYILEKGYNSMPINLPGTLFHKAMKARKELAQILANILSKRRQNP 245

Query: 247 KELVEKNLLGCLLKWRDENGESLSDEQIADNIIGVLFAAQDTTASVLTWVLKYLHDNQKV 306
                 +LLG  +    E+   L+DEQIADNIIGV+FAA+DTTASVLTW+LKYL DN  V
Sbjct: 246 SS--HTDLLGSFM----EDKAGLTDEQIADNIIGVIFAARDTTASVLTWILKYLADNPTV 305

Query: 307 LEAVKAEQKAIEECIDEGSRPMSWTQTRNMSFTNKVVLESLRMASIISFTFREAVTDVEY 366
           LEAV  EQ AI +   EG   ++W  T+ M  T +V+ E+LR A+I+SFTFREAV DVEY
Sbjct: 306 LEAVTEEQMAIRKDKKEG-ESLTWEDTKKMPLTYRVIQETLRAATILSFTFREAVEDVEY 365

Query: 367 NGYLIPKGWKVMPLFRNIHHNPDYFVDPHKFDPSRFEVAPRPNTFMPFGGGVHACPGNEL 426
            GYLIPKGWKV+PLFRNIHHN D F DP KFDPSRFEVAP+PNTFMPFG G+H+CPGNEL
Sbjct: 366 EGYLIPKGWKVLPLFRNIHHNADIFSDPGKFDPSRFEVAPKPNTFMPFGSGIHSCPGNEL 425

Query: 427 AKLEILIMIHHLVTKFRWELAGERDGIQHGPFPVPMDGLPIRVWR 472
           AKLEI ++IHHL TK+RW + G  DGIQ+GPF +P +GLPI + R
Sbjct: 426 AKLEISVLIHHLTTKYRWSIVGPSDGIQYGPFALPQNGLPIALER 461

The following BLAST results are available for this feature:
BLAST of Lag0004287 vs. NCBI nr
Match NameE-valueIdentityDescription
XP_038884199.12.0e-24288.49abscisic acid 8'-hydroxylase CYP707A1-like [Benincasa hispida][more]
XP_022962256.15.0e-23383.90abscisic acid 8'-hydroxylase 3-like [Cucurbita moschata][more]
XP_023546528.16.5e-23383.72abscisic acid 8'-hydroxylase 3-like [Cucurbita pepo subsp. pepo][more]
XP_022144125.13.3e-22983.40abscisic acid 8'-hydroxylase 4-like [Momordica charantia][more]
KAA0060904.11.3e-22889.17abscisic acid 8'-hydroxylase 4-like [Cucumis melo var. makuwa][more]
BLAST of Lag0004287 vs. ExPASy Swiss-Prot
Match NameE-valueIdentityDescription
A9QNE77.6e-18466.45Abscisic acid 8'-hydroxylase CYP707A1 OS=Solanum lycopersicum OX=4081 GN=CYP707A... [more]
Q9LJK21.1e-17966.59Abscisic acid 8'-hydroxylase 4 OS=Arabidopsis thaliana OX=3702 GN=CYP707A4 PE=2 ... [more]
Q0J1851.9e-17967.88Abscisic acid 8'-hydroxylase 3 OS=Oryza sativa subsp. japonica OX=39947 GN=CYP70... [more]
A2Z2127.3e-17967.42Abscisic acid 8'-hydroxylase 3 OS=Oryza sativa subsp. indica OX=39946 GN=CYP707A... [more]
Q09J789.9e-17665.45Abscisic acid 8'-hydroxylase 2 OS=Oryza sativa subsp. indica OX=39946 GN=CYP707A... [more]
BLAST of Lag0004287 vs. ExPASy TrEMBL
Match NameE-valueIdentityDescription
A0A6J1HCL52.4e-23383.90abscisic acid 8'-hydroxylase 3-like OS=Cucurbita moschata OX=3662 GN=LOC11146276... [more]
A0A6J1CQS31.6e-22983.40abscisic acid 8'-hydroxylase 4-like OS=Momordica charantia OX=3673 GN=LOC1110138... [more]
A0A5A7V0Q46.1e-22989.17Abscisic acid 8'-hydroxylase 4-like OS=Cucumis melo var. makuwa OX=1194695 GN=E6... [more]
A0A0A0LKV63.4e-22788.30Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_2G357280 PE=3 SV=1[more]
A0A6J1K9Y77.0e-22586.81abscisic acid 8'-hydroxylase 3-like OS=Cucurbita maxima OX=3661 GN=LOC111492434 ... [more]
BLAST of Lag0004287 vs. TAIR 10
Match NameE-valueIdentityDescription
AT3G19270.18.0e-18166.59cytochrome P450, family 707, subfamily A, polypeptide 4 [more]
AT2G29090.18.4e-15458.53cytochrome P450, family 707, subfamily A, polypeptide 2 [more]
AT2G29090.28.4e-15458.53cytochrome P450, family 707, subfamily A, polypeptide 2 [more]
AT4G19230.18.4e-15456.40cytochrome P450, family 707, subfamily A, polypeptide 1 [more]
AT5G45340.11.1e-15355.91cytochrome P450, family 707, subfamily A, polypeptide 3 [more]
InterPro
Analysis Name: InterPro Annotations of Sponge gourd (AG-4) v1
Date Performed: 2022-08-01
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR001128Cytochrome P450PRINTSPR00385P450coord: 421..432
score: 39.37
coord: 341..352
score: 33.62
coord: 284..301
score: 35.09
coord: 412..421
score: 47.83
IPR001128Cytochrome P450PFAMPF00067p450coord: 38..450
e-value: 1.9E-70
score: 237.9
IPR002401Cytochrome P450, E-class, group IPRINTSPR00463EP450Icoord: 380..404
score: 28.31
coord: 273..290
score: 21.19
coord: 293..319
score: 20.6
coord: 411..421
score: 46.77
coord: 421..444
score: 28.29
coord: 66..85
score: 31.32
coord: 340..358
score: 23.92
IPR036396Cytochrome P450 superfamilyGENE3D1.10.630.10Cytochrome P450coord: 26..472
e-value: 2.2E-121
score: 407.7
IPR036396Cytochrome P450 superfamilySUPERFAMILY48264Cytochrome P450coord: 35..470
NoneNo IPR availablePANTHERPTHR24286:SF103CYTOCHROME P450 FAMILY ABA 8'-HYDROXYLASEcoord: 17..470
NoneNo IPR availablePANTHERPTHR24286CYTOCHROME P450 26coord: 17..470
IPR017972Cytochrome P450, conserved sitePROSITEPS00086CYTOCHROME_P450coord: 414..423

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Lag0004287.1Lag0004287.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0016125 sterol metabolic process
cellular_component GO:0016021 integral component of membrane
molecular_function GO:0010295 (+)-abscisic acid 8'-hydroxylase activity
molecular_function GO:0020037 heme binding
molecular_function GO:0005506 iron ion binding
molecular_function GO:0004497 monooxygenase activity
molecular_function GO:0016705 oxidoreductase activity, acting on paired donors, with incorporation or reduction of molecular oxygen