Lag0004282 (gene) Sponge gourd (AG‐4) v1

Overview
NameLag0004282
Typegene
OrganismLuffa acutangula (Sponge gourd (AG‐4) v1)
Descriptionglutamate receptor 2.7-like
Locationchr6: 2555910 .. 2558838 (+)
RNA-Seq ExpressionLag0004282
SyntenyLag0004282
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonCDSpolypeptide
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGCATTTAATTCCTAAGTTTGACAAATCCAATGAAGATATTGCCACCATATCACTACCAACTGCTGCTTTTGTTCCTCCTCTAATGTCGGATCAACTTCATCCGCCCTCCTTTATTCAAATGGCTCACGACATTAAATATCACATACAGTGCATAGCTTCCATCGTTGGCCATTTCCAGTGGCATAAAGTTACTATAATCTACGAGAACAAGAACGACATGTCGGTGAACAACTTGGAGACTTTGACTCTTCTCTCCAATGAACTTCAAGTTTTCAATGCAGAGATCGAGCAAATTTCAGAATTCTCTTCCTCACATTCAGAAGCTACGATTGAGGAGAAGCTAAAAAGTCTTGTGGGTCATGAGAGGAACAATGTCTTCATTGTGCTGCAATTTTCCATAGAGTTGGCCAAACTCCTTTTTCATAAAGCAAATCAAATGAACATGATGGAGAATGGATTTGTTTGGATTGTTGGAGATGAGATATCAAGCCTTGTCGACTCTTTAGATTCATCTACTTTGTATAACATGCAAGGTGTCATAGGATATCGAACTTACTTTGACCATTCCAAAGATTCAATCAAGCAATTCAAAAGCAAATTTCGTAGAATATATCTTTCAAAATATCAAGTACAAGAAGAAGATGAAGAGATCAAGAATGGCGAGCCCAGCATCTTTGCCCTTCGAGCTTATGATGCAACATTGGCTGTGACCATAGCCATGCGAAGGTTGCAAGGAATAATGTTCAGTAGTGAACAATTTTTGCAGGAAGTTTTAGCCATTGAATTTGAAGGCCTTAGTGGGAAGATAGGATTCGAGAATGGTGTATTGATGCAACCGCCAACTTTTGAAATCATTTACGTGGTGGGAAAGAGCTACAAAGAGATGGGATTTTGGAGACAAAAGGTTGGATTTTTATTCAACAACTTGATGGAAGATGAAGTGAGCATTATTATTAGTGGAAGAGGTAGAAAAGGCCTTTTGGATTTGTCAAATTGGGGAGCTCCAAAGCGACGGATTAATCTTGATTATTCTAATTCTAAATTAGACAGAGCCTTAAAAATTGGGGTTCCAGCCAACAGTACTTTTCGAGAATTTGTGAAAGTATCTTACCATCACATTAATGGGATTTACATCTCTGGATTTTCTATTTCTGTGTTTGAAGCTGTTGTAAAAAACTTACCTTATCCTTTGTCTTACCAGTTGGTCCCCTTCAATGGCTCCTATGATGGATTGGTAAAGCAAGTCTACGAAAAGGTTTGTTCTCTTTAAGATACATTTTACTTCTTTGTTTCCAACATGAACAATACTTTTACCTACACATCATAATTAAATTCAGTAGAACTCATACTTTCTAGAGTGTAGTGTAGACAAGGATTTAAGACTATCATAAATTTATAGGAAATTGCATTAGGTGGCCATTAGGGTTTTTTTAATAAGTAATAGCAAATATGGCAATTTCTTAGAATAAAATTTTATAATTTCCGTTTCACTCCTATTTAATTTTGAATTGTGTGTGAATTCTACCAATTTCCATACCATTCATTTTTATCAGTGTCTATCACCAATAGACTTTGATAATCGGAGTCTATTAGTGTTGATAGACTCTGTATAATTCTTTCTATCCGTTCTATCATTGATAGATTCTGATAAACGAAGTCTATCGATGTTTATCATCGATAGATTTTGATTGACTATCAGTGATAGACACTGATAAAATATCAATTAGATTTAGTATGATCTTTTTTGTCAGTGATAGACTCTGATTAATTCAGTGTATTAGTGACTATCAGTGATAAATTACTGATAAACTATCATGTTGATAGACTTCGTATAATCCTTTCTATCATTGTTGGTCATTGATAGACTCCTATTAATCAAAGTTTATCAATGATAGACACTAATAATTTTTTCTATTAGATAGTTGCTAATAAAAAAGAGTATTTTTGTAACTTAATGAGTCTATCAATGACTATCAATGATAGACACTAATAATTTTTTCTATTAGATAGTTGCTAATAAAAAAGAGTATTTTTGTAACTTAACGAGTCTATCAATGACTATCAATGATAGACACTGATAATTTTTTCTATTAGATAGTTGCTAATTAAAAATAGTATTTTTGTAACTTAATGTCACTTTGTGCTATTTCTGTAAATAGTTTTGGTTTTTGCTATTTTTGCAATATATTTAGTACTTTTTGCTATATGTGGAAATGCCCCTAAATTTATTGATGTATATATACTGCCTTTTTATTAGGGTTTGGATGCTGCGGTGGGAGACATAGGAATATATGCAGATCGATTTCAATACGTTGATTTTTCAGAGCCGTATTTGGTATCTGGCCTTATGATGATAGTGAAAGAGGAGAAAGAGAATTGGAAAGAAATATGGATATTCTTGAAAACATTTACGATGAAAATGTGGATAATATTGCCATTGTCACATATATTTATAATCTCTATCGTTTGGTTCGTTAAAGAAGACAGCGAAGAGTTAAGATCTGGATTCGGAAATATGCTCTGGTTTGGCATATCTGTCATCTTTTATGCACAAAGTAATTAAAAGATATAAGTGGCATGGTTTCAACAATAGTCACAAGTACTTCTTGTGTCATGAAGCTAACAACAATTTTCATAAACCCCACGCAGGAGAACAAGTGAATGGTGGGTTGGCTCGGTTGGTGTTAGGACCATGGTTGTTTGTAATTCTTGTGGTAACCTCAAGTTTCACTGCAAGTCTCACCTCTATGATGACAGTTTCAAGATTTTCCCCATCAGTTGTAGATATTGAAACATTAAGGCAAACAAATGCAACTGTGGGCTGCAACTTTCATTCTTTCATCATGCGATATCTAACCAATGTGTTGCACATTCCTCTTGATAATATTAAGACCCCTGTTGGCATTGATGATTATCCAAAGTGTTGA

mRNA sequence

ATGCATTTAATTCCTAAGTTTGACAAATCCAATGAAGATATTGCCACCATATCACTACCAACTGCTGCTTTTGTTCCTCCTCTAATGTCGGATCAACTTCATCCGCCCTCCTTTATTCAAATGGCTCACGACATTAAATATCACATACAGTGCATAGCTTCCATCGTTGGCCATTTCCAGTGGCATAAAGTTACTATAATCTACGAGAACAAGAACGACATGTCGGTGAACAACTTGGAGACTTTGACTCTTCTCTCCAATGAACTTCAAGTTTTCAATGCAGAGATCGAGCAAATTTCAGAATTCTCTTCCTCACATTCAGAAGCTACGATTGAGGAGAAGCTAAAAAGTCTTGTGGGTCATGAGAGGAACAATGTCTTCATTGTGCTGCAATTTTCCATAGAGTTGGCCAAACTCCTTTTTCATAAAGCAAATCAAATGAACATGATGGAGAATGGATTTGTTTGGATTGTTGGAGATGAGATATCAAGCCTTGTCGACTCTTTAGATTCATCTACTTTGTATAACATGCAAGGTGTCATAGGATATCGAACTTACTTTGACCATTCCAAAGATTCAATCAAGCAATTCAAAAGCAAATTTCGTAGAATATATCTTTCAAAATATCAAGTACAAGAAGAAGATGAAGAGATCAAGAATGGCGAGCCCAGCATCTTTGCCCTTCGAGCTTATGATGCAACATTGGCTGTGACCATAGCCATGCGAAGGTTGCAAGGAATAATGTTCAGTAGTGAACAATTTTTGCAGGAAGTTTTAGCCATTGAATTTGAAGGCCTTAGTGGGAAGATAGGATTCGAGAATGGTGTATTGATGCAACCGCCAACTTTTGAAATCATTTACGTGGTGGGAAAGAGCTACAAAGAGATGGGATTTTGGAGACAAAAGGTTGGATTTTTATTCAACAACTTGATGGAAGATGAAGTGAGCATTATTATTAGTGGAAGAGGTAGAAAAGGCCTTTTGGATTTGTCAAATTGGGGAGCTCCAAAGCGACGGATTAATCTTGATTATTCTAATTCTAAATTAGACAGAGCCTTAAAAATTGGGGTTCCAGCCAACAGTACTTTTCGAGAATTTGTGAAAGTATCTTACCATCACATTAATGGGATTTACATCTCTGGATTTTCTATTTCTGTGTTTGAAGCTGTTGTAAAAAACTTACCTTATCCTTTGTCTTACCAGTTGGTCCCCTTCAATGGCTCCTATGATGGATTGGTAAAGCAAGTCTACGAAAAGGGTTTGGATGCTGCGGTGGGAGACATAGGAATATATGCAGATCGATTTCAATACGTTGATTTTTCAGAGCCGTATTTGGTATCTGGCCTTATGATGATAGTGAAAGAGGAGAAAGAGAATTGGAAAGAAATATGGATATTCTTGAAAACATTTACGATGAAAATGTGGATAATATTGCCATTGTCACATATATTTATAATCTCTATCGTTTGGTTCGTTAAAGAAGACAGCGAAGAGTTAAGATCTGGATTCGGAAATATGCTCTGGTTTGGCATATCTGTCATCTTTTATGCACAAAGAGAACAAGTGAATGGTGGGTTGGCTCGGTTGGTGTTAGGACCATGGTTGTTTGTAATTCTTGTGGTAACCTCAAGTTTCACTGCAAGTCTCACCTCTATGATGACAGTTTCAAGATTTTCCCCATCAGTTGTAGATATTGAAACATTAAGGCAAACAAATGCAACTGTGGGCTGCAACTTTCATTCTTTCATCATGCGATATCTAACCAATGTGTTGCACATTCCTCTTGATAATATTAAGACCCCTGTTGGCATTGATGATTATCCAAAGTGTTGA

Coding sequence (CDS)

ATGCATTTAATTCCTAAGTTTGACAAATCCAATGAAGATATTGCCACCATATCACTACCAACTGCTGCTTTTGTTCCTCCTCTAATGTCGGATCAACTTCATCCGCCCTCCTTTATTCAAATGGCTCACGACATTAAATATCACATACAGTGCATAGCTTCCATCGTTGGCCATTTCCAGTGGCATAAAGTTACTATAATCTACGAGAACAAGAACGACATGTCGGTGAACAACTTGGAGACTTTGACTCTTCTCTCCAATGAACTTCAAGTTTTCAATGCAGAGATCGAGCAAATTTCAGAATTCTCTTCCTCACATTCAGAAGCTACGATTGAGGAGAAGCTAAAAAGTCTTGTGGGTCATGAGAGGAACAATGTCTTCATTGTGCTGCAATTTTCCATAGAGTTGGCCAAACTCCTTTTTCATAAAGCAAATCAAATGAACATGATGGAGAATGGATTTGTTTGGATTGTTGGAGATGAGATATCAAGCCTTGTCGACTCTTTAGATTCATCTACTTTGTATAACATGCAAGGTGTCATAGGATATCGAACTTACTTTGACCATTCCAAAGATTCAATCAAGCAATTCAAAAGCAAATTTCGTAGAATATATCTTTCAAAATATCAAGTACAAGAAGAAGATGAAGAGATCAAGAATGGCGAGCCCAGCATCTTTGCCCTTCGAGCTTATGATGCAACATTGGCTGTGACCATAGCCATGCGAAGGTTGCAAGGAATAATGTTCAGTAGTGAACAATTTTTGCAGGAAGTTTTAGCCATTGAATTTGAAGGCCTTAGTGGGAAGATAGGATTCGAGAATGGTGTATTGATGCAACCGCCAACTTTTGAAATCATTTACGTGGTGGGAAAGAGCTACAAAGAGATGGGATTTTGGAGACAAAAGGTTGGATTTTTATTCAACAACTTGATGGAAGATGAAGTGAGCATTATTATTAGTGGAAGAGGTAGAAAAGGCCTTTTGGATTTGTCAAATTGGGGAGCTCCAAAGCGACGGATTAATCTTGATTATTCTAATTCTAAATTAGACAGAGCCTTAAAAATTGGGGTTCCAGCCAACAGTACTTTTCGAGAATTTGTGAAAGTATCTTACCATCACATTAATGGGATTTACATCTCTGGATTTTCTATTTCTGTGTTTGAAGCTGTTGTAAAAAACTTACCTTATCCTTTGTCTTACCAGTTGGTCCCCTTCAATGGCTCCTATGATGGATTGGTAAAGCAAGTCTACGAAAAGGGTTTGGATGCTGCGGTGGGAGACATAGGAATATATGCAGATCGATTTCAATACGTTGATTTTTCAGAGCCGTATTTGGTATCTGGCCTTATGATGATAGTGAAAGAGGAGAAAGAGAATTGGAAAGAAATATGGATATTCTTGAAAACATTTACGATGAAAATGTGGATAATATTGCCATTGTCACATATATTTATAATCTCTATCGTTTGGTTCGTTAAAGAAGACAGCGAAGAGTTAAGATCTGGATTCGGAAATATGCTCTGGTTTGGCATATCTGTCATCTTTTATGCACAAAGAGAACAAGTGAATGGTGGGTTGGCTCGGTTGGTGTTAGGACCATGGTTGTTTGTAATTCTTGTGGTAACCTCAAGTTTCACTGCAAGTCTCACCTCTATGATGACAGTTTCAAGATTTTCCCCATCAGTTGTAGATATTGAAACATTAAGGCAAACAAATGCAACTGTGGGCTGCAACTTTCATTCTTTCATCATGCGATATCTAACCAATGTGTTGCACATTCCTCTTGATAATATTAAGACCCCTGTTGGCATTGATGATTATCCAAAGTGTTGA

Protein sequence

MHLIPKFDKSNEDIATISLPTAAFVPPLMSDQLHPPSFIQMAHDIKYHIQCIASIVGHFQWHKVTIIYENKNDMSVNNLETLTLLSNELQVFNAEIEQISEFSSSHSEATIEEKLKSLVGHERNNVFIVLQFSIELAKLLFHKANQMNMMENGFVWIVGDEISSLVDSLDSSTLYNMQGVIGYRTYFDHSKDSIKQFKSKFRRIYLSKYQVQEEDEEIKNGEPSIFALRAYDATLAVTIAMRRLQGIMFSSEQFLQEVLAIEFEGLSGKIGFENGVLMQPPTFEIIYVVGKSYKEMGFWRQKVGFLFNNLMEDEVSIIISGRGRKGLLDLSNWGAPKRRINLDYSNSKLDRALKIGVPANSTFREFVKVSYHHINGIYISGFSISVFEAVVKNLPYPLSYQLVPFNGSYDGLVKQVYEKGLDAAVGDIGIYADRFQYVDFSEPYLVSGLMMIVKEEKENWKEIWIFLKTFTMKMWIILPLSHIFIISIVWFVKEDSEELRSGFGNMLWFGISVIFYAQREQVNGGLARLVLGPWLFVILVVTSSFTASLTSMMTVSRFSPSVVDIETLRQTNATVGCNFHSFIMRYLTNVLHIPLDNIKTPVGIDDYPKC
Homology
BLAST of Lag0004282 vs. NCBI nr
Match: XP_023546340.1 (glutamate receptor 2.5-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 891.7 bits (2303), Expect = 3.5e-255
Identity = 461/619 (74.47%), Postives = 523/619 (84.49%), Query Frame = 0

Query: 1   MHLIPKFDKSNEDIATISLPTAAFVPPLMSDQLHPPSFIQMAHDIKYHIQCIASIVGHFQ 60
           M LI + +K++ DIATISLP AA VPPL    L PPSFIQMAH I +H+QC A++VGHFQ
Sbjct: 106 MQLISEINKTSVDIATISLPVAASVPPL----LPPPSFIQMAHHITFHMQCAAALVGHFQ 165

Query: 61  WHKVTIIYENKNDMSVNNLETLTLLSNELQVFNAEIEQISEFSSSHSEATIEEKLKSLVG 120
           WHKVT+IYEN+NDMS+ N+E LTLLSNEL+VFNAEIEQIS FSSSH+EA IEEKLKSL G
Sbjct: 166 WHKVTVIYENRNDMSI-NMEALTLLSNELRVFNAEIEQISAFSSSHTEAMIEEKLKSLTG 225

Query: 121 HERNNVFIVLQFSIELAKLLFHKANQMNMMENGFVWIVGDEISSLVDSLDSSTLYNMQGV 180
            +RN VFIV+QFSIELAKLLFH+A +MNMM+NGFVWIVGDE+SSL+DSLDSS  YNMQGV
Sbjct: 226 RDRNRVFIVVQFSIELAKLLFHRAKRMNMMDNGFVWIVGDEMSSLMDSLDSSNFYNMQGV 285

Query: 181 IGYRTYFDHSKDSIKQFKSKFRRIYLSKYQ--VQEEDEEIKNGEPSIFALRAYDATLAVT 240
           IG+RTY DH+KDS K+F+SKFRR+Y  +YQ   +EE+EE K+ EPSIFALRAYDA+ AV 
Sbjct: 286 IGFRTYVDHTKDSFKKFRSKFRRMYRLEYQHHDEEEEEEEKSAEPSIFALRAYDASWAVA 345

Query: 241 IAMRRLQGIMFSSEQFLQEVLAIEFEGLSGKIGFENGVLMQPPTFEIIYVVGKSYKEMGF 300
            AM +LQG  FS++Q L+++LA EFEGLSG IGFENG L QPPTFEIIYVVGKSYKEMGF
Sbjct: 346 AAMNKLQG-NFSNKQLLKQILATEFEGLSGSIGFENGTLKQPPTFEIIYVVGKSYKEMGF 405

Query: 301 WRQKVGFLFNNLMEDEVSIIISGRGRKGLLDLSNW--------GAPKRRINLDYSNSKLD 360
           WRQKVGF FNNLMEDE         R G+L+   +           KRR+NLD SNS++ 
Sbjct: 406 WRQKVGF-FNNLMEDE-------EIRNGVLEFPRFVFWEGNEQTGLKRRMNLD-SNSEVP 465

Query: 361 RALKIGVPANSTFREFVKVSYHHINGIYISGFSISVFEAVVKNLPYPLSYQLVPFNGSYD 420
           R LKIGVPAN+TF EFVKVSY HING+YISG+SISVFEAVVKNLPYPL Y LVPFNGSYD
Sbjct: 466 RILKIGVPANNTFHEFVKVSYDHINGVYISGYSISVFEAVVKNLPYPLHYHLVPFNGSYD 525

Query: 421 GLVKQVYEKGLDAAVGDIGIYADRFQYVDFSEPYLVSGLMMIVKEEKENWKEIWIFLKTF 480
            LVKQV+ KGLDAAVGDIGI+ADRFQYVDF+E Y+VSGL+MIVKEEK NWKEIW+F+KTF
Sbjct: 526 ELVKQVHAKGLDAAVGDIGIFADRFQYVDFTESYMVSGLLMIVKEEKRNWKEIWVFMKTF 585

Query: 481 TMKMWIILPLSHIFIISIVWFVKEDSEELRSGFGNMLWFGISVIFYAQREQVNGGLARLV 540
           T  MWIILPLSH+FIIS+VWFV+ +SE L+SGFG+MLWF ISV+F A RE+V GGLARLV
Sbjct: 586 TTTMWIILPLSHMFIISVVWFVRPESERLKSGFGDMLWFAISVVFNAHREEVGGGLARLV 645

Query: 541 LGPWLFVILVVTSSFTASLTSMMTVSRFSPSVVDIETLRQTNATVGCNFHSFIMRYLTNV 600
           L PWLFVILVVTSSFTASLTSMMTVSRF+PSVVDIETLRQTNATVGCNFHSFIMRYL NV
Sbjct: 646 LAPWLFVILVVTSSFTASLTSMMTVSRFAPSVVDIETLRQTNATVGCNFHSFIMRYLNNV 705

Query: 601 LHIPLDNIKTPVGIDDYPK 610
           L I  DNIKT   IDDYPK
Sbjct: 706 LQISPDNIKTLSSIDDYPK 709

BLAST of Lag0004282 vs. NCBI nr
Match: KAG6598371.1 (Glutamate receptor 2.5, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 884.4 bits (2284), Expect = 5.6e-253
Identity = 461/619 (74.47%), Postives = 520/619 (84.01%), Query Frame = 0

Query: 3    LIPKFDKSNEDIATISLPTAAFVPPLMSDQLHPPSFIQMAHDIKYHIQCIASIVGHFQWH 62
            LI + +K++ DIATISLP AA VPPL    L PPSFIQMAH I +H+QC A+IVGHFQWH
Sbjct: 1002 LISEINKTSVDIATISLPVAASVPPL----LPPPSFIQMAHHITFHMQCAAAIVGHFQWH 1061

Query: 63   KVTIIYENKNDMSVNNLETLTLLSNELQVFNAEIEQISEFSSSHSEATIEEKLKSLVGHE 122
            KVT+IYEN+NDMS+ N+E LTLLSNEL+VFNAEIEQIS FSSSH+EA IEEKLKSL G E
Sbjct: 1062 KVTVIYENRNDMSI-NMEALTLLSNELRVFNAEIEQISAFSSSHTEAMIEEKLKSLKGRE 1121

Query: 123  RNNVFIVLQFSIELAKLLFHKANQMNMMENGFVWIVGDEISSLVDSLDSSTLYNMQGVIG 182
            RN VFIV+QFSIELAKLLFH+A +MNMM+NGFVWIVGDEISSL+DSLDSS  YNMQGVIG
Sbjct: 1122 RNRVFIVVQFSIELAKLLFHRAKRMNMMDNGFVWIVGDEISSLMDSLDSSNFYNMQGVIG 1181

Query: 183  YRTYFDHSKDSIKQFKSKFRRIYLSKYQ----VQEEDEEIKNGEPSIFALRAYDATLAVT 242
            +RTYF+HSKDS K+F+ KFRR+Y  +YQ     +EE+EE K+ EPSIFALRAYDA+ AV 
Sbjct: 1182 FRTYFNHSKDSFKKFRRKFRRMYRLEYQHHDEEEEEEEEEKSAEPSIFALRAYDASWAVA 1241

Query: 243  IAMRRLQGIMFSSEQFLQEVLAIEFEGLSGKIGFENGVLMQPPTFEIIYVVGKSYKEMGF 302
             AM  LQG  FS++Q L+++LA EFEGLSG IGFENG L QPPTFEIIYVVGKSYKEMGF
Sbjct: 1242 AAMDILQG-NFSNKQLLKQILATEFEGLSGSIGFENGTLKQPPTFEIIYVVGKSYKEMGF 1301

Query: 303  WRQKVGFLFNNLMEDEVSIIISGRGRKGLLDLSNW--------GAPKRRINLDYSNSKLD 362
            WRQKVGF FN+LMEDE         R G+L+   +           KRR+NLD SNS+  
Sbjct: 1302 WRQKVGF-FNSLMEDE-------EIRNGVLEFPRFVFWEGNEQTGLKRRMNLD-SNSEEH 1361

Query: 363  RALKIGVPANSTFREFVKVSYHHINGIYISGFSISVFEAVVKNLPYPLSYQLVPFNGSYD 422
            R LKIGVPAN+TF EFVKVSY HING+YISG+SISVFEAVVKNLPYPL Y LVPFNGSYD
Sbjct: 1362 RILKIGVPANNTFHEFVKVSYDHINGVYISGYSISVFEAVVKNLPYPLHYHLVPFNGSYD 1421

Query: 423  GLVKQVYEKGLDAAVGDIGIYADRFQYVDFSEPYLVSGLMMIVKEEKENWKEIWIFLKTF 482
             LVKQV+ KGLDAAVGDIGI+ADRFQYVDF+E Y+VSGL+MIVKEEK NWKEIW+F+KTF
Sbjct: 1422 ELVKQVHAKGLDAAVGDIGIFADRFQYVDFTESYMVSGLLMIVKEEKRNWKEIWVFMKTF 1481

Query: 483  TMKMWIILPLSHIFIISIVWFVKEDSEELRSGFGNMLWFGISVIFYAQREQVNGGLARLV 542
            T  MWIILPLSH+FIIS+VWFV+ +SE L+SGFG+MLWF ISV+F + RE+V GGLARLV
Sbjct: 1482 TTTMWIILPLSHMFIISVVWFVRPESERLKSGFGDMLWFAISVVFNSHREEVGGGLARLV 1541

Query: 543  LGPWLFVILVVTSSFTASLTSMMTVSRFSPSVVDIETLRQTNATVGCNFHSFIMRYLTNV 602
            L PWLFVILVVTSSFTASLTSMMTVSRF+PSVVDIETLRQTNATVGCNFHSFIMRYL NV
Sbjct: 1542 LAPWLFVILVVTSSFTASLTSMMTVSRFAPSVVDIETLRQTNATVGCNFHSFIMRYLNNV 1601

Query: 603  LHIPLDNIKTPVGIDDYPK 610
            L I  DNIKT   IDDYPK
Sbjct: 1602 LQISPDNIKTLSSIDDYPK 1605

BLAST of Lag0004282 vs. NCBI nr
Match: XP_022997324.1 (glutamate receptor 2.5-like isoform X3 [Cucurbita maxima])

HSP 1 Score: 879.8 bits (2272), Expect = 1.4e-251
Identity = 456/619 (73.67%), Postives = 520/619 (84.01%), Query Frame = 0

Query: 1   MHLIPKFDKSNEDIATISLPTAAFVPPLMSDQLHPPSFIQMAHDIKYHIQCIASIVGHFQ 60
           M  I + +K++ DIATISLP AA VPPL    L  PSFIQMAH I +H+QC A+IVGHFQ
Sbjct: 106 MQFISEINKTSVDIATISLPVAASVPPL----LPLPSFIQMAHHITFHMQCAAAIVGHFQ 165

Query: 61  WHKVTIIYENKNDMSVNNLETLTLLSNELQVFNAEIEQISEFSSSHSEATIEEKLKSLVG 120
           WHKVT+IYEN+NDMS+ N+E  TLLSNEL+VFNAEIEQIS FSSSH+EA IEEKLKSL+G
Sbjct: 166 WHKVTVIYENRNDMSI-NMEAFTLLSNELRVFNAEIEQISAFSSSHTEAMIEEKLKSLMG 225

Query: 121 HERNNVFIVLQFSIELAKLLFHKANQMNMMENGFVWIVGDEISSLVDSLDSSTLYNMQGV 180
            +RN VFIV+QFSIELAKLLFH+A +MNMM+NGFVWIVGDEISSL+DSLDSS  YNMQ V
Sbjct: 226 RDRNRVFIVVQFSIELAKLLFHRAKRMNMMDNGFVWIVGDEISSLMDSLDSSNFYNMQDV 285

Query: 181 IGYRTYFDHSKDSIKQFKSKFRRIYLSKYQ--VQEEDEEIKNGEPSIFALRAYDATLAVT 240
           IG+RTYF+HSKDS K+F+ KFRR+Y  +YQ   +EE+EE K+ EPSIFALRAYDA+ AV 
Sbjct: 286 IGFRTYFNHSKDSFKKFRRKFRRMYRLEYQHHDEEEEEEEKSAEPSIFALRAYDASWAVA 345

Query: 241 IAMRRLQGIMFSSEQFLQEVLAIEFEGLSGKIGFENGVLMQPPTFEIIYVVGKSYKEMGF 300
            AM +LQG  F+++Q L+++LA EFEGLSG IGFENG L QPPTFEIIYVVGKSYKEMGF
Sbjct: 346 AAMNKLQG-NFTNKQLLKQILATEFEGLSGSIGFENGTLKQPPTFEIIYVVGKSYKEMGF 405

Query: 301 WRQKVGFLFNNLMEDEVSIIISGRGRKGLLDLSNW--------GAPKRRINLDYSNSKLD 360
           WRQKVGF FN+LMEDE         + G+L+   +           KRR+NLD SNS+  
Sbjct: 406 WRQKVGF-FNSLMEDE-------EIKNGVLEFPRFVFWEGNEQTGLKRRMNLD-SNSEEH 465

Query: 361 RALKIGVPANSTFREFVKVSYHHINGIYISGFSISVFEAVVKNLPYPLSYQLVPFNGSYD 420
           R LKIGVPAN+TF EFVKVSY HING+YISG+SISVFEAVVKNLPYPL YQLVPFNGSYD
Sbjct: 466 RILKIGVPANNTFHEFVKVSYDHINGVYISGYSISVFEAVVKNLPYPLHYQLVPFNGSYD 525

Query: 421 GLVKQVYEKGLDAAVGDIGIYADRFQYVDFSEPYLVSGLMMIVKEEKENWKEIWIFLKTF 480
            LVKQV+ KGLDAAVGDIGI+ADRFQYVDF+E Y+VSGL+MIVKEEK NWKEIW+F+KTF
Sbjct: 526 ELVKQVHAKGLDAAVGDIGIFADRFQYVDFTESYMVSGLLMIVKEEKRNWKEIWVFMKTF 585

Query: 481 TMKMWIILPLSHIFIISIVWFVKEDSEELRSGFGNMLWFGISVIFYAQREQVNGGLARLV 540
           T  MWIILP+SH+FIIS+VWFV+ +SE L+SGFG+MLWF ISV+F A RE+V GGLARLV
Sbjct: 586 TTTMWIILPMSHMFIISVVWFVRPESERLKSGFGDMLWFAISVVFNAHREEVGGGLARLV 645

Query: 541 LGPWLFVILVVTSSFTASLTSMMTVSRFSPSVVDIETLRQTNATVGCNFHSFIMRYLTNV 600
           L PWLFVILVVTSSFTASLTSMMTVSRF+PSVVDIETLRQTNATVGCNFHSFIMRYL NV
Sbjct: 646 LAPWLFVILVVTSSFTASLTSMMTVSRFAPSVVDIETLRQTNATVGCNFHSFIMRYLNNV 705

Query: 601 LHIPLDNIKTPVGIDDYPK 610
           L I  DNIKT   IDDYPK
Sbjct: 706 LQISPDNIKTLSSIDDYPK 709

BLAST of Lag0004282 vs. NCBI nr
Match: XP_022997321.1 (glutamate receptor 2.5-like isoform X1 [Cucurbita maxima])

HSP 1 Score: 879.8 bits (2272), Expect = 1.4e-251
Identity = 456/619 (73.67%), Postives = 520/619 (84.01%), Query Frame = 0

Query: 1   MHLIPKFDKSNEDIATISLPTAAFVPPLMSDQLHPPSFIQMAHDIKYHIQCIASIVGHFQ 60
           M  I + +K++ DIATISLP AA VPPL    L  PSFIQMAH I +H+QC A+IVGHFQ
Sbjct: 106 MQFISEINKTSVDIATISLPVAASVPPL----LPLPSFIQMAHHITFHMQCAAAIVGHFQ 165

Query: 61  WHKVTIIYENKNDMSVNNLETLTLLSNELQVFNAEIEQISEFSSSHSEATIEEKLKSLVG 120
           WHKVT+IYEN+NDMS+ N+E  TLLSNEL+VFNAEIEQIS FSSSH+EA IEEKLKSL+G
Sbjct: 166 WHKVTVIYENRNDMSI-NMEAFTLLSNELRVFNAEIEQISAFSSSHTEAMIEEKLKSLMG 225

Query: 121 HERNNVFIVLQFSIELAKLLFHKANQMNMMENGFVWIVGDEISSLVDSLDSSTLYNMQGV 180
            +RN VFIV+QFSIELAKLLFH+A +MNMM+NGFVWIVGDEISSL+DSLDSS  YNMQ V
Sbjct: 226 RDRNRVFIVVQFSIELAKLLFHRAKRMNMMDNGFVWIVGDEISSLMDSLDSSNFYNMQDV 285

Query: 181 IGYRTYFDHSKDSIKQFKSKFRRIYLSKYQ--VQEEDEEIKNGEPSIFALRAYDATLAVT 240
           IG+RTYF+HSKDS K+F+ KFRR+Y  +YQ   +EE+EE K+ EPSIFALRAYDA+ AV 
Sbjct: 286 IGFRTYFNHSKDSFKKFRRKFRRMYRLEYQHHDEEEEEEEKSAEPSIFALRAYDASWAVA 345

Query: 241 IAMRRLQGIMFSSEQFLQEVLAIEFEGLSGKIGFENGVLMQPPTFEIIYVVGKSYKEMGF 300
            AM +LQG  F+++Q L+++LA EFEGLSG IGFENG L QPPTFEIIYVVGKSYKEMGF
Sbjct: 346 AAMNKLQG-NFTNKQLLKQILATEFEGLSGSIGFENGTLKQPPTFEIIYVVGKSYKEMGF 405

Query: 301 WRQKVGFLFNNLMEDEVSIIISGRGRKGLLDLSNW--------GAPKRRINLDYSNSKLD 360
           WRQKVGF FN+LMEDE         + G+L+   +           KRR+NLD SNS+  
Sbjct: 406 WRQKVGF-FNSLMEDE-------EIKNGVLEFPRFVFWEGNEQTGLKRRMNLD-SNSEEH 465

Query: 361 RALKIGVPANSTFREFVKVSYHHINGIYISGFSISVFEAVVKNLPYPLSYQLVPFNGSYD 420
           R LKIGVPAN+TF EFVKVSY HING+YISG+SISVFEAVVKNLPYPL YQLVPFNGSYD
Sbjct: 466 RILKIGVPANNTFHEFVKVSYDHINGVYISGYSISVFEAVVKNLPYPLHYQLVPFNGSYD 525

Query: 421 GLVKQVYEKGLDAAVGDIGIYADRFQYVDFSEPYLVSGLMMIVKEEKENWKEIWIFLKTF 480
            LVKQV+ KGLDAAVGDIGI+ADRFQYVDF+E Y+VSGL+MIVKEEK NWKEIW+F+KTF
Sbjct: 526 ELVKQVHAKGLDAAVGDIGIFADRFQYVDFTESYMVSGLLMIVKEEKRNWKEIWVFMKTF 585

Query: 481 TMKMWIILPLSHIFIISIVWFVKEDSEELRSGFGNMLWFGISVIFYAQREQVNGGLARLV 540
           T  MWIILP+SH+FIIS+VWFV+ +SE L+SGFG+MLWF ISV+F A RE+V GGLARLV
Sbjct: 586 TTTMWIILPMSHMFIISVVWFVRPESERLKSGFGDMLWFAISVVFNAHREEVGGGLARLV 645

Query: 541 LGPWLFVILVVTSSFTASLTSMMTVSRFSPSVVDIETLRQTNATVGCNFHSFIMRYLTNV 600
           L PWLFVILVVTSSFTASLTSMMTVSRF+PSVVDIETLRQTNATVGCNFHSFIMRYL NV
Sbjct: 646 LAPWLFVILVVTSSFTASLTSMMTVSRFAPSVVDIETLRQTNATVGCNFHSFIMRYLNNV 705

Query: 601 LHIPLDNIKTPVGIDDYPK 610
           L I  DNIKT   IDDYPK
Sbjct: 706 LQISPDNIKTLSSIDDYPK 709

BLAST of Lag0004282 vs. NCBI nr
Match: XP_022997323.1 (glutamate receptor 2.5-like isoform X2 [Cucurbita maxima])

HSP 1 Score: 879.8 bits (2272), Expect = 1.4e-251
Identity = 456/619 (73.67%), Postives = 520/619 (84.01%), Query Frame = 0

Query: 1   MHLIPKFDKSNEDIATISLPTAAFVPPLMSDQLHPPSFIQMAHDIKYHIQCIASIVGHFQ 60
           M  I + +K++ DIATISLP AA VPPL    L  PSFIQMAH I +H+QC A+IVGHFQ
Sbjct: 106 MQFISEINKTSVDIATISLPVAASVPPL----LPLPSFIQMAHHITFHMQCAAAIVGHFQ 165

Query: 61  WHKVTIIYENKNDMSVNNLETLTLLSNELQVFNAEIEQISEFSSSHSEATIEEKLKSLVG 120
           WHKVT+IYEN+NDMS+ N+E  TLLSNEL+VFNAEIEQIS FSSSH+EA IEEKLKSL+G
Sbjct: 166 WHKVTVIYENRNDMSI-NMEAFTLLSNELRVFNAEIEQISAFSSSHTEAMIEEKLKSLMG 225

Query: 121 HERNNVFIVLQFSIELAKLLFHKANQMNMMENGFVWIVGDEISSLVDSLDSSTLYNMQGV 180
            +RN VFIV+QFSIELAKLLFH+A +MNMM+NGFVWIVGDEISSL+DSLDSS  YNMQ V
Sbjct: 226 RDRNRVFIVVQFSIELAKLLFHRAKRMNMMDNGFVWIVGDEISSLMDSLDSSNFYNMQDV 285

Query: 181 IGYRTYFDHSKDSIKQFKSKFRRIYLSKYQ--VQEEDEEIKNGEPSIFALRAYDATLAVT 240
           IG+RTYF+HSKDS K+F+ KFRR+Y  +YQ   +EE+EE K+ EPSIFALRAYDA+ AV 
Sbjct: 286 IGFRTYFNHSKDSFKKFRRKFRRMYRLEYQHHDEEEEEEEKSAEPSIFALRAYDASWAVA 345

Query: 241 IAMRRLQGIMFSSEQFLQEVLAIEFEGLSGKIGFENGVLMQPPTFEIIYVVGKSYKEMGF 300
            AM +LQG  F+++Q L+++LA EFEGLSG IGFENG L QPPTFEIIYVVGKSYKEMGF
Sbjct: 346 AAMNKLQG-NFTNKQLLKQILATEFEGLSGSIGFENGTLKQPPTFEIIYVVGKSYKEMGF 405

Query: 301 WRQKVGFLFNNLMEDEVSIIISGRGRKGLLDLSNW--------GAPKRRINLDYSNSKLD 360
           WRQKVGF FN+LMEDE         + G+L+   +           KRR+NLD SNS+  
Sbjct: 406 WRQKVGF-FNSLMEDE-------EIKNGVLEFPRFVFWEGNEQTGLKRRMNLD-SNSEEH 465

Query: 361 RALKIGVPANSTFREFVKVSYHHINGIYISGFSISVFEAVVKNLPYPLSYQLVPFNGSYD 420
           R LKIGVPAN+TF EFVKVSY HING+YISG+SISVFEAVVKNLPYPL YQLVPFNGSYD
Sbjct: 466 RILKIGVPANNTFHEFVKVSYDHINGVYISGYSISVFEAVVKNLPYPLHYQLVPFNGSYD 525

Query: 421 GLVKQVYEKGLDAAVGDIGIYADRFQYVDFSEPYLVSGLMMIVKEEKENWKEIWIFLKTF 480
            LVKQV+ KGLDAAVGDIGI+ADRFQYVDF+E Y+VSGL+MIVKEEK NWKEIW+F+KTF
Sbjct: 526 ELVKQVHAKGLDAAVGDIGIFADRFQYVDFTESYMVSGLLMIVKEEKRNWKEIWVFMKTF 585

Query: 481 TMKMWIILPLSHIFIISIVWFVKEDSEELRSGFGNMLWFGISVIFYAQREQVNGGLARLV 540
           T  MWIILP+SH+FIIS+VWFV+ +SE L+SGFG+MLWF ISV+F A RE+V GGLARLV
Sbjct: 586 TTTMWIILPMSHMFIISVVWFVRPESERLKSGFGDMLWFAISVVFNAHREEVGGGLARLV 645

Query: 541 LGPWLFVILVVTSSFTASLTSMMTVSRFSPSVVDIETLRQTNATVGCNFHSFIMRYLTNV 600
           L PWLFVILVVTSSFTASLTSMMTVSRF+PSVVDIETLRQTNATVGCNFHSFIMRYL NV
Sbjct: 646 LAPWLFVILVVTSSFTASLTSMMTVSRFAPSVVDIETLRQTNATVGCNFHSFIMRYLNNV 705

Query: 601 LHIPLDNIKTPVGIDDYPK 610
           L I  DNIKT   IDDYPK
Sbjct: 706 LQISPDNIKTLSSIDDYPK 709

BLAST of Lag0004282 vs. ExPASy Swiss-Prot
Match: Q9LFN5 (Glutamate receptor 2.5 OS=Arabidopsis thaliana OX=3702 GN=GLR2.5 PE=2 SV=2)

HSP 1 Score: 251.5 bits (641), Expect = 2.4e-65
Identity = 181/600 (30.17%), Postives = 297/600 (49.50%), Query Frame = 0

Query: 27  PLMSDQLHPPSFIQMAHDIKYHIQCIASIVGHFQWHKVTIIYENKNDMSVNNLETLTLLS 86
           PL+ D L  P FI+  HD    +Q I++I+  F+W +V  IY + N+     L  L    
Sbjct: 136 PLL-DSLRSPYFIRATHDDSSQVQAISAIIESFRWREVVPIYVD-NEFGEGILPNLV--- 195

Query: 87  NELQVFNAEIEQISEFSSSHSEATIEEKLKSLVGHERNNVFIVLQFSIELAKLLFHKANQ 146
           +  Q  N  I   S  S  +S+  I+++L  L+      VFIV     +L   LF  A +
Sbjct: 196 DAFQEINVRIRYRSAISLHYSDDQIKKELYKLMTMP-TRVFIVHMLP-DLGSRLFSIAKE 255

Query: 147 MNMMENGFVWIVGDEISSLVDSLDSSTLYNMQGVIGYRTYFDHSKDSIKQFKSKFRRIYL 206
           ++M+  G+VWIV + I+ L+  +  S+L NM GV+G +TYF  SK+ +            
Sbjct: 256 IDMLSKGYVWIVTNGIADLMSIMGESSLVNMHGVLGVKTYFAKSKELLH---------LE 315

Query: 207 SKYQVQEEDEEIKNGEPSIFALRAYDATLAVTIAMRRLQ--------------------- 266
           +++Q +   EE+ N     FA  AYDA  A+ +++  ++                     
Sbjct: 316 ARWQKRFGGEELNN-----FACWAYDAATALAMSVEEIRHVNMSFNTTKEDTSRDDIGTD 375

Query: 267 ----GIMFSSEQFLQEVLAIEFEGLSGKIGFENGVLMQPPTFEIIYVVGKSYKEMGFWRQ 326
               G+  S  + L  +  + F+G++G+   +NG L +  TF+II +     + +GFW+ 
Sbjct: 376 LDELGVALSGPKLLDALSTVSFKGVAGRFQLKNGKL-EATTFKIINIEESGERTVGFWKS 435

Query: 327 KVGFLFNNLMEDEVSIIISGRGRKGLLDLSNWGAPKRRINLDYSNSKLDRALKIGVPANS 386
           KVG L  +L  D+VS   S R  + ++    W      +   +      + L+I VP   
Sbjct: 436 KVG-LVKSLRVDKVS--HSSRRLRPII----WPGDTIFVPKGWEFPTNAKKLRIAVPKKD 495

Query: 387 TFREFVKVSY-HHINGIYISGFSISVFEAVVKNLPYPLSYQLVPFN-------GSYDGLV 446
            F  FV+V+   + N   ++GF I VF  V+  +PY +SY+ +PF+       GSYD +V
Sbjct: 496 GFNNFVEVTKDENTNVPTVTGFCIDVFNTVMSQMPYAVSYEYIPFDTPDGKPRGSYDEMV 555

Query: 447 KQVYEKGLDAAVGDIGIYADRFQYVDFSEPYLVSGLMMIVKEEKENWKEIWIFLKTFTMK 506
             V+    D AVGD  I A+R  YVDF+ PY  +G++ +V  +    K  W+FLK  T +
Sbjct: 556 YNVFLGEFDGAVGDTTILANRSHYVDFALPYSETGIVFLVPVKDGKEKGEWVFLKPLTKE 615

Query: 507 MWIILPLSHIFIISIVW-FVKEDSEELR-----SGFGNMLWFGISVIFYAQREQVNGGLA 566
           +W++   S ++I  +VW F  +  EE R         ++ +F  S +F+A R        
Sbjct: 616 LWLVTAASFLYIGIMVWIFEYQADEEFREQMIIDKISSVFYFSFSTLFFAHRRPSESFFT 675

Query: 567 RLVLGPWLFVILVVTSSFTASLTSMMTVSRFSPSVVDIETLRQTNATVGCNFHSFIMRYL 588
           R+++  W FV+L++T S+TA+LTSM+TV    P+V  ++ LR++   +G    SF    L
Sbjct: 676 RVLVVVWCFVLLILTQSYTATLTSMLTVQELRPTVRHMDDLRKSGVNIGYQTGSFTFERL 706

BLAST of Lag0004282 vs. ExPASy Swiss-Prot
Match: O04660 (Glutamate receptor 2.1 OS=Arabidopsis thaliana OX=3702 GN=GLR2.1 PE=2 SV=2)

HSP 1 Score: 242.3 bits (617), Expect = 1.5e-62
Identity = 186/610 (30.49%), Postives = 298/610 (48.85%), Query Frame = 0

Query: 3   LIPKFDKSNEDIATISLPTAAFVPPLMSDQLHPPSFIQMAHDIKYHIQCIASIVGHFQWH 62
           +I    KS   I T S    A  P L S  +    F +  +D    +  I  I+  F W 
Sbjct: 112 MIEMGQKSQVPIVTYS----ATSPSLAS--IRSQYFFRATYDDSSQVHAIKEIIKLFGWR 171

Query: 63  KVTIIYENKNDMSVNNLETLTLLSNELQVFNAEIEQISEFSSSHSEATIEEKLKSLVGHE 122
           +V  +Y +           +  L++ LQ  N  I   +  S + ++  I  +L  ++   
Sbjct: 172 EVAPVYVD----DTFGEGIMPRLTDVLQEINVRIPYRTVISPNATDDEISVELLRMMTLP 231

Query: 123 RNNVFIVLQFSIELAKLLFHKANQMNMMENGFVWIVGDEISSLVDSLDSSTLYNMQGVIG 182
              VF+V    + LA   F KA ++ +M+ G+VWI+ + I+ ++  ++ + +  MQGV+G
Sbjct: 232 -TRVFVVHLVEL-LASRFFAKATEIGLMKQGYVWILTNTITDVLSIMNETEIETMQGVLG 291

Query: 183 YRTYFDHSKDSIKQFKSKFRRIYLSKYQVQEEDEEIKNGEPSIFALRAYDATLAVTIAMR 242
            +TY   SK+ ++ F+S++ +                  + +++ L AYDAT A+ +A+ 
Sbjct: 292 VKTYVPRSKE-LENFRSRWTK-------------RFPISDLNVYGLWAYDATTALALAIE 351

Query: 243 -------------------RLQGIMFS--SEQFLQEVLAIEFEGLSGKIGFENGVLMQPP 302
                               LQG+  S    + LQ +  + F+GL+G   F NG L QP 
Sbjct: 352 EAGTSNLTFVKMDAKRNVSELQGLGVSQYGPKLLQTLSRVRFQGLAGDFQFINGEL-QPS 411

Query: 303 TFEIIYVVGKSYKEMGFWRQKVGFLFNNLMEDEVSIIISGRGRKGLLDLSNWGAPKRRIN 362
            FEI+ V G+  + +GFW ++ G LF N+ +   S       +  L  +  W      + 
Sbjct: 412 VFEIVNVNGQGGRTIGFWMKEYG-LFKNVDQKPASKTTFSSWQDRLRPII-WPGDTTSVP 471

Query: 363 LDYSNSKLDRALKIGVPANSTFREFVKVSYHHI-NGIYISGFSISVFEAVVKNLPYPLSY 422
             +      + L+IGVP N+TF++FVK +   I N    SGFSI  FEAV++ +PY +SY
Sbjct: 472 KGWEIPTNGKRLQIGVPVNNTFQQFVKATRDPITNSTIFSGFSIDYFEAVIQAIPYDISY 531

Query: 423 QLVPF-NGSYDGLVKQVYEKGLDAAVGDIGIYADRFQYVDFSEPYLVSGLMMIVKEEKEN 482
             +PF +G YD LV QVY    DA V D  I ++R  YVDFS PY  SG+ ++V  +   
Sbjct: 532 DFIPFQDGGYDALVYQVYLGKYDAVVADTTISSNRSMYVDFSLPYTPSGVGLVVPVKDSV 591

Query: 483 WKEIWIFLKTFTMKMWIILPLSHIFIISIVWFVKEDSEELRSGFG-----NMLWFGISVI 542
            +   IFL   T+ +W+I  LS   I  +VW ++        G G      + WF  S++
Sbjct: 592 RRSSTIFLMPLTLALWLISLLSFFIIGLVVWVLEHRVNPDFDGPGQYQLSTIFWFSFSIM 651

Query: 543 FYAQREQVNGGLARLVLGPWLFVILVVTSSFTASLTSMMTVSRFSPSVVDIETLRQTNAT 585
            +A RE+V    AR+V+  W F++LV+T S+TASL S++T     P+V +I +L     +
Sbjct: 652 VFAPRERVLSFWARVVVIIWYFLVLVLTQSYTASLASLLTTQHLHPTVTNINSLLAKGES 691

BLAST of Lag0004282 vs. ExPASy Swiss-Prot
Match: Q9SHV2 (Glutamate receptor 2.3 OS=Arabidopsis thaliana OX=3702 GN=GLR2.3 PE=2 SV=1)

HSP 1 Score: 235.7 bits (600), Expect = 1.4e-60
Identity = 166/587 (28.28%), Postives = 292/587 (49.74%), Query Frame = 0

Query: 33  LHPPSFIQMAHDIKYHIQCIASIVGHFQWHKVTIIYENKNDMSVNNLETLTLLSNELQVF 92
           L  P F++  ++  + +Q I +I+  F W +V  +Y + N      +  LT   + LQ  
Sbjct: 135 LRSPYFLRATYEDSFQVQPIKAIIKLFGWREVVPVYID-NTFGEGIMPRLT---DALQDI 194

Query: 93  NAEIEQISEFSSSHSEATIEEKLKSLVGHERNNVFIVLQFSIELAKLLFHKANQMNMMEN 152
           N  I   S  + + ++  I  +L  ++ +    VF+V  +  +LA   F KA ++ +ME 
Sbjct: 195 NVRIPYRSVIAINATDHEISVELLKMM-NMPTRVFLVHMY-YDLASRFFIKAKELGLMEP 254

Query: 153 GFVWIVGDEISSLVDSLDSSTLYNMQGVIGYRTYFDHSKDSIKQFKSKFRRIYLSKYQVQ 212
           G+VWI+ + +   +  ++ + +  M+GV+G +TY   S D +++F+S++R ++       
Sbjct: 255 GYVWILTNGVIDDLSLINETAVEAMEGVLGIKTYIPKSPD-LEKFRSRWRSLF------- 314

Query: 213 EEDEEIKNGEPSIFALRAYDATLAVTIAMR----------------------RLQGIMFS 272
                    E S++ L AYDAT A+ +A+                          G+   
Sbjct: 315 ------PRVELSVYGLWAYDATTALAVAIEEAGTNNMTFSKVVDTGRNVSELEALGLSQF 374

Query: 273 SEQFLQEVLAIEFEGLSGKIGFENGVLMQPPTFEIIYVVGKSYKEMGFWRQKVGFLFNNL 332
             + LQ +L ++F GL+G+  F  G L QP  FEI+ ++    K +GFW++  G +    
Sbjct: 375 GPKLLQTLLTVQFRGLAGEFRFFRGQL-QPSVFEIVNIINTGEKSIGFWKEGNGLV--KK 434

Query: 333 MEDEVSIIISGRGRKGLLDLSNWGAPKRRINLDYSNSKLDRALKIGVPANSTFREFVKVS 392
           ++ + S I +    K  L    W      +   +      + L+IGVP  + + + VKV+
Sbjct: 435 LDQQASSISALSTWKDHLKHIVWPGEADSVPKGWQIPTKGKKLRIGVPKRTGYTDLVKVT 494

Query: 393 YHHI-NGIYISGFSISVFEAVVKNLPYPLSYQLVPFN-------GSYDGLVKQVYEKGLD 452
              I N   ++GF I  FEAV++ LPY +SY+ +PF        G+Y+ LV QVY    D
Sbjct: 495 RDPITNSTVVTGFCIDFFEAVIRELPYDVSYEFIPFEKPDGKTAGNYNDLVYQVYLGRYD 554

Query: 453 AAVGDIGIYADRFQYVDFSEPYLVSGLMMIVKEEKENWKEIWIFLKTFTMKMWIILPLSH 512
           A VGD  I  +R  YVDF+ P++ SG+ +IV+      ++  +F+K  + K+W+   +S 
Sbjct: 555 AVVGDTTILVNRSSYVDFTFPFIKSGVGLIVEMTDPVKRDYILFMKPLSWKLWLTSFISF 614

Query: 513 IFIISIVWFVK-----EDSEELRSGFGNMLWFGISVIFYAQREQVNGGLARLVLGPWLFV 572
             +   VW ++     + S   R     + WF  S + +A RE+V    AR ++  W F+
Sbjct: 615 FLVGCTVWVLEYKRNPDFSGPPRFQASTICWFAFSTMVFAPRERVFSFWARALVIAWYFL 674

Query: 573 ILVVTSSFTASLTSMMTVSRFSPSVVDIETLRQTNATVGCNFHSFIM 585
           +LV+T S+TASL S++T  + +P++  + +L +   TVG    SFI+
Sbjct: 675 VLVLTQSYTASLASLLTSQKLNPTITSMSSLLEKGETVGYQRTSFIL 698

BLAST of Lag0004282 vs. ExPASy Swiss-Prot
Match: Q9LFN8 (Glutamate receptor 2.6 OS=Arabidopsis thaliana OX=3702 GN=GLR2.6 PE=2 SV=2)

HSP 1 Score: 235.7 bits (600), Expect = 1.4e-60
Identity = 167/583 (28.64%), Postives = 282/583 (48.37%), Query Frame = 0

Query: 31  DQLHPPSFIQMAHDIKYHIQCIASIVGHFQWHKVTIIYENKNDMSVNNLETLTLLSNELQ 90
           D L  P FI+  HD    +  I++I+  F+W +V  IY + N+        L  L +  Q
Sbjct: 138 DSLRSPYFIRATHDDSSQVHAISAIIESFRWREVVPIYAD-NEFGEG---ILPYLVDAFQ 197

Query: 91  VFNAEIEQISEFSSSHSEATIEEKLKSLVGHERNNVFIVLQFSIELAKLLFHKANQMNMM 150
             N  I   S  S   ++  ++++L  L+      VFIV     +L   LF  A ++ MM
Sbjct: 198 EINVRIRYRSAISVHSTDDLVKKELYKLMTMP-TRVFIVHMLP-DLGSRLFSIAKEIGMM 257

Query: 151 ENGFVWIVGDEISSLVDSLDSSTLYNMQGVIGYRTYFDHSKDSI---KQFKSKFRRIYLS 210
             G+VWIV + I+  +  +  S+L NM GV+G +TYF  SK+ +    +++ +F    L+
Sbjct: 258 TKGYVWIVTNGIADQMSVMGESSLENMHGVLGVKTYFSRSKELMYLETRWRKRFGGEELN 317

Query: 211 KYQVQEED---------EEIKNGEPSIFALRAYDATLAVTIAMRRLQGIMFSSEQFLQEV 270
            ++    D         EEI +     F+    + +   T           S  + LQ +
Sbjct: 318 NFECWGYDTATALAMSIEEISSNVNMSFSQTKRNTSRDDTGTDLDDLSFALSGPKLLQAL 377

Query: 271 LAIEFEGLSGKIGFENGVLMQPPTFEIIYVVGKSYKEMGFWRQKVGFLFNNLMEDEVSII 330
             + F+G++G+   +NG L +  TF+I+ +     + +GFW+ KVG L  +L  ++  I 
Sbjct: 378 ATVSFKGVAGRFQLKNGKL-EATTFKIVNIEESGERTVGFWKSKVG-LVKSLRVNQTGIK 437

Query: 331 ISGRGRKGLLDLSNWGAPKRRINLDYSNSKLDRALKIGVPANSTFREFVKVSYH-HINGI 390
           IS    +  L    W      +   +      + L+I VP    F  FV+V+   + N  
Sbjct: 438 ISHSSHR--LRPIIWPGDTIFVPKGWEFPTNAKKLRIAVPKKDGFNNFVEVTKDANTNAP 497

Query: 391 YISGFSISVFEAVVKNLPYPLSYQLVPF-------NGSYDGLVKQVYEKGLDAAVGDIGI 450
            I+GF I VF+  ++ +PY + Y+ +PF        GSYD +V  V+    D AVGD  I
Sbjct: 498 TITGFCIDVFDTAMRQMPYAVPYEYIPFETPDGKPRGSYDEMVYHVFLGEFDGAVGDTTI 557

Query: 451 YADRFQYVDFSEPYLVSGLMMIVKEEKENWKEIWIFLKTFTMKMWIILPLSHIFIISIVW 510
            A+R  YVDF+ PY  +G++++V  + E  K  W+FLK  T ++W +   S ++I  +VW
Sbjct: 558 LANRSTYVDFALPYSETGIVVVVPVKDEREKGKWVFLKPLTRELWFLTAASFLYIGIMVW 617

Query: 511 FVKEDS------EELRSGFGNMLWFGISVIFYAQREQVNGGLARLVLGPWLFVILVVTSS 570
             +  +      + + +   N+ +F  S +F+A          R+++  W FV+L++T S
Sbjct: 618 IFEYQASGDFRKQSIINKISNVFYFSFSTLFFAHMRPSESIFTRVLVVVWCFVLLILTQS 677

Query: 571 FTASLTSMMTVSRFSPSVVDIETLRQTNATVGCNFHSFIMRYL 588
           +TA+LTSM+TV    P+V  ++ LR +   +G    SF    L
Sbjct: 678 YTATLTSMLTVQELRPTVRHMDDLRNSGVNIGYQTGSFTFERL 710

BLAST of Lag0004282 vs. ExPASy Swiss-Prot
Match: Q8LGN0 (Glutamate receptor 2.7 OS=Arabidopsis thaliana OX=3702 GN=GLR2.7 PE=2 SV=3)

HSP 1 Score: 234.2 bits (596), Expect = 4.0e-60
Identity = 179/603 (29.68%), Postives = 294/603 (48.76%), Query Frame = 0

Query: 19  LPTAAF--VPPLMSDQLHPPSFIQMAHDIKYHIQCIASIVGHFQWHKVTIIYENKNDMSV 78
           +PT  F    PL++  ++ P F++   D    ++ IA+IV  F W  V  IY + N+   
Sbjct: 128 VPTITFSATCPLLT-SINSPYFVRATLDDSSQVKAIAAIVKSFGWRNVVAIYVD-NEFGE 187

Query: 79  NNLETLTLLSNELQVFNAEIEQISEFSSSHSEATIEEKLKSLVGHERNNVFIVLQFSIEL 138
             L  LT    ++Q F      I +   ++ +  ++E  K +    R  VF+V      L
Sbjct: 188 GILPLLTDALQDVQAFVVNRCLIPQ--EANDDQILKELYKLMTMQTR--VFVV-HMPPTL 247

Query: 139 AKLLFHKANQMNMMENGFVWIVGDEISSLVDSLD-SSTLYNMQGVIGYRTYFDHSKDSIK 198
               F KA ++ MME G+VW++ D + +L+ S +  S+L NMQGV+G R++   SK  +K
Sbjct: 248 GFRFFQKAREIGMMEEGYVWLLTDGVMNLLKSNERGSSLENMQGVLGVRSHIPKSK-KLK 307

Query: 199 QFKSKFRRIYLSKYQVQEEDEEIKNGEPSIFALRAYDATLAVTIAMRRLQ---------- 258
            F+ ++ +++  K      DEE+     +IFALRAYD+  A+ +A+ +            
Sbjct: 308 NFRLRWEKMFPKK----GNDEEM-----NIFALRAYDSITALAMAVEKTNIKSLRYDHPI 367

Query: 259 ------------GIMFSSEQFLQEVLAIEFEGLSGKIGFENGVLMQPPTFEIIYVVGKSY 318
                       G+       L+ +  + F GL+G+    NG L +   F++I ++G   
Sbjct: 368 ASGNNKTNLGTLGVSRYGPSLLKALSNVRFNGLAGEFELINGQL-ESSVFDVINIIGSEE 427

Query: 319 KEMGFWRQKVGFLFNNLMEDEVSIIISGRGRKGLLDLSNWGAPKRRINLDYSNSKLDRAL 378
           + +G WR   G +  N      + ++  R     L    W    + +   +      + L
Sbjct: 428 RIIGLWRPSNGIV--NAKSKNTTSVLGER-----LGPVIWPGKSKDVPKGWQIPTNGKML 487

Query: 379 KIGVPANSTFREFVKVSYHHI-NGIYISGFSISVFEAVVKNLPYPLSYQLVPF---NGSY 438
           ++G+P    F EFV      I N +  +G+ I +FEAV+K LPY +  + + F   + +Y
Sbjct: 488 RVGIPVKKGFLEFVDAKIDPISNAMTPTGYCIEIFEAVLKKLPYSVIPKYIAFLSPDENY 547

Query: 439 DGLVKQVYEKGLDAAVGDIGIYADRFQYVDFSEPYLVSGLMMIVKEEKENWKEIWIFLKT 498
           D +V QVY    DA VGD+ I A+R  YVDF+ PY  SG+ M+V   K+N K  W+FL+ 
Sbjct: 548 DEMVYQVYTGAYDAVVGDVTIVANRSLYVDFTLPYTESGVSMMV-PLKDN-KNTWVFLRP 607

Query: 499 FTMKMWIILPLSHIFIISIVWFVKEDSEELRSG-----FGNMLWFGISVIFYAQREQVNG 558
           +++ +W+      +FI  IVW ++        G      G   WF  S + +A RE+V  
Sbjct: 608 WSLDLWVTTACFFVFIGFIVWILEHRVNTDFRGPPHHQIGTSFWFAFSTMNFAHREKVVS 667

Query: 559 GLARLVLGPWLFVILVVTSSFTASLTSMMTVSRFSPSVVDIETLRQTNATVGCNFHSFIM 588
            LAR V+  W FV+LV+  S+TA+LTS  TV    P+V + + L + N  +G    +F+ 
Sbjct: 668 NLARFVVLVWCFVVLVLIQSYTANLTSFFTVKLLQPTVTNWKDLIKFNKNIGYQRGTFVR 703

BLAST of Lag0004282 vs. ExPASy TrEMBL
Match: A0A6J1K765 (glutamate receptor 2.5-like isoform X1 OS=Cucurbita maxima OX=3661 GN=LOC111492269 PE=3 SV=1)

HSP 1 Score: 879.8 bits (2272), Expect = 6.6e-252
Identity = 456/619 (73.67%), Postives = 520/619 (84.01%), Query Frame = 0

Query: 1   MHLIPKFDKSNEDIATISLPTAAFVPPLMSDQLHPPSFIQMAHDIKYHIQCIASIVGHFQ 60
           M  I + +K++ DIATISLP AA VPPL    L  PSFIQMAH I +H+QC A+IVGHFQ
Sbjct: 106 MQFISEINKTSVDIATISLPVAASVPPL----LPLPSFIQMAHHITFHMQCAAAIVGHFQ 165

Query: 61  WHKVTIIYENKNDMSVNNLETLTLLSNELQVFNAEIEQISEFSSSHSEATIEEKLKSLVG 120
           WHKVT+IYEN+NDMS+ N+E  TLLSNEL+VFNAEIEQIS FSSSH+EA IEEKLKSL+G
Sbjct: 166 WHKVTVIYENRNDMSI-NMEAFTLLSNELRVFNAEIEQISAFSSSHTEAMIEEKLKSLMG 225

Query: 121 HERNNVFIVLQFSIELAKLLFHKANQMNMMENGFVWIVGDEISSLVDSLDSSTLYNMQGV 180
            +RN VFIV+QFSIELAKLLFH+A +MNMM+NGFVWIVGDEISSL+DSLDSS  YNMQ V
Sbjct: 226 RDRNRVFIVVQFSIELAKLLFHRAKRMNMMDNGFVWIVGDEISSLMDSLDSSNFYNMQDV 285

Query: 181 IGYRTYFDHSKDSIKQFKSKFRRIYLSKYQ--VQEEDEEIKNGEPSIFALRAYDATLAVT 240
           IG+RTYF+HSKDS K+F+ KFRR+Y  +YQ   +EE+EE K+ EPSIFALRAYDA+ AV 
Sbjct: 286 IGFRTYFNHSKDSFKKFRRKFRRMYRLEYQHHDEEEEEEEKSAEPSIFALRAYDASWAVA 345

Query: 241 IAMRRLQGIMFSSEQFLQEVLAIEFEGLSGKIGFENGVLMQPPTFEIIYVVGKSYKEMGF 300
            AM +LQG  F+++Q L+++LA EFEGLSG IGFENG L QPPTFEIIYVVGKSYKEMGF
Sbjct: 346 AAMNKLQG-NFTNKQLLKQILATEFEGLSGSIGFENGTLKQPPTFEIIYVVGKSYKEMGF 405

Query: 301 WRQKVGFLFNNLMEDEVSIIISGRGRKGLLDLSNW--------GAPKRRINLDYSNSKLD 360
           WRQKVGF FN+LMEDE         + G+L+   +           KRR+NLD SNS+  
Sbjct: 406 WRQKVGF-FNSLMEDE-------EIKNGVLEFPRFVFWEGNEQTGLKRRMNLD-SNSEEH 465

Query: 361 RALKIGVPANSTFREFVKVSYHHINGIYISGFSISVFEAVVKNLPYPLSYQLVPFNGSYD 420
           R LKIGVPAN+TF EFVKVSY HING+YISG+SISVFEAVVKNLPYPL YQLVPFNGSYD
Sbjct: 466 RILKIGVPANNTFHEFVKVSYDHINGVYISGYSISVFEAVVKNLPYPLHYQLVPFNGSYD 525

Query: 421 GLVKQVYEKGLDAAVGDIGIYADRFQYVDFSEPYLVSGLMMIVKEEKENWKEIWIFLKTF 480
            LVKQV+ KGLDAAVGDIGI+ADRFQYVDF+E Y+VSGL+MIVKEEK NWKEIW+F+KTF
Sbjct: 526 ELVKQVHAKGLDAAVGDIGIFADRFQYVDFTESYMVSGLLMIVKEEKRNWKEIWVFMKTF 585

Query: 481 TMKMWIILPLSHIFIISIVWFVKEDSEELRSGFGNMLWFGISVIFYAQREQVNGGLARLV 540
           T  MWIILP+SH+FIIS+VWFV+ +SE L+SGFG+MLWF ISV+F A RE+V GGLARLV
Sbjct: 586 TTTMWIILPMSHMFIISVVWFVRPESERLKSGFGDMLWFAISVVFNAHREEVGGGLARLV 645

Query: 541 LGPWLFVILVVTSSFTASLTSMMTVSRFSPSVVDIETLRQTNATVGCNFHSFIMRYLTNV 600
           L PWLFVILVVTSSFTASLTSMMTVSRF+PSVVDIETLRQTNATVGCNFHSFIMRYL NV
Sbjct: 646 LAPWLFVILVVTSSFTASLTSMMTVSRFAPSVVDIETLRQTNATVGCNFHSFIMRYLNNV 705

Query: 601 LHIPLDNIKTPVGIDDYPK 610
           L I  DNIKT   IDDYPK
Sbjct: 706 LQISPDNIKTLSSIDDYPK 709

BLAST of Lag0004282 vs. ExPASy TrEMBL
Match: A0A6J1KB40 (glutamate receptor 2.5-like isoform X3 OS=Cucurbita maxima OX=3661 GN=LOC111492269 PE=3 SV=1)

HSP 1 Score: 879.8 bits (2272), Expect = 6.6e-252
Identity = 456/619 (73.67%), Postives = 520/619 (84.01%), Query Frame = 0

Query: 1   MHLIPKFDKSNEDIATISLPTAAFVPPLMSDQLHPPSFIQMAHDIKYHIQCIASIVGHFQ 60
           M  I + +K++ DIATISLP AA VPPL    L  PSFIQMAH I +H+QC A+IVGHFQ
Sbjct: 106 MQFISEINKTSVDIATISLPVAASVPPL----LPLPSFIQMAHHITFHMQCAAAIVGHFQ 165

Query: 61  WHKVTIIYENKNDMSVNNLETLTLLSNELQVFNAEIEQISEFSSSHSEATIEEKLKSLVG 120
           WHKVT+IYEN+NDMS+ N+E  TLLSNEL+VFNAEIEQIS FSSSH+EA IEEKLKSL+G
Sbjct: 166 WHKVTVIYENRNDMSI-NMEAFTLLSNELRVFNAEIEQISAFSSSHTEAMIEEKLKSLMG 225

Query: 121 HERNNVFIVLQFSIELAKLLFHKANQMNMMENGFVWIVGDEISSLVDSLDSSTLYNMQGV 180
            +RN VFIV+QFSIELAKLLFH+A +MNMM+NGFVWIVGDEISSL+DSLDSS  YNMQ V
Sbjct: 226 RDRNRVFIVVQFSIELAKLLFHRAKRMNMMDNGFVWIVGDEISSLMDSLDSSNFYNMQDV 285

Query: 181 IGYRTYFDHSKDSIKQFKSKFRRIYLSKYQ--VQEEDEEIKNGEPSIFALRAYDATLAVT 240
           IG+RTYF+HSKDS K+F+ KFRR+Y  +YQ   +EE+EE K+ EPSIFALRAYDA+ AV 
Sbjct: 286 IGFRTYFNHSKDSFKKFRRKFRRMYRLEYQHHDEEEEEEEKSAEPSIFALRAYDASWAVA 345

Query: 241 IAMRRLQGIMFSSEQFLQEVLAIEFEGLSGKIGFENGVLMQPPTFEIIYVVGKSYKEMGF 300
            AM +LQG  F+++Q L+++LA EFEGLSG IGFENG L QPPTFEIIYVVGKSYKEMGF
Sbjct: 346 AAMNKLQG-NFTNKQLLKQILATEFEGLSGSIGFENGTLKQPPTFEIIYVVGKSYKEMGF 405

Query: 301 WRQKVGFLFNNLMEDEVSIIISGRGRKGLLDLSNW--------GAPKRRINLDYSNSKLD 360
           WRQKVGF FN+LMEDE         + G+L+   +           KRR+NLD SNS+  
Sbjct: 406 WRQKVGF-FNSLMEDE-------EIKNGVLEFPRFVFWEGNEQTGLKRRMNLD-SNSEEH 465

Query: 361 RALKIGVPANSTFREFVKVSYHHINGIYISGFSISVFEAVVKNLPYPLSYQLVPFNGSYD 420
           R LKIGVPAN+TF EFVKVSY HING+YISG+SISVFEAVVKNLPYPL YQLVPFNGSYD
Sbjct: 466 RILKIGVPANNTFHEFVKVSYDHINGVYISGYSISVFEAVVKNLPYPLHYQLVPFNGSYD 525

Query: 421 GLVKQVYEKGLDAAVGDIGIYADRFQYVDFSEPYLVSGLMMIVKEEKENWKEIWIFLKTF 480
            LVKQV+ KGLDAAVGDIGI+ADRFQYVDF+E Y+VSGL+MIVKEEK NWKEIW+F+KTF
Sbjct: 526 ELVKQVHAKGLDAAVGDIGIFADRFQYVDFTESYMVSGLLMIVKEEKRNWKEIWVFMKTF 585

Query: 481 TMKMWIILPLSHIFIISIVWFVKEDSEELRSGFGNMLWFGISVIFYAQREQVNGGLARLV 540
           T  MWIILP+SH+FIIS+VWFV+ +SE L+SGFG+MLWF ISV+F A RE+V GGLARLV
Sbjct: 586 TTTMWIILPMSHMFIISVVWFVRPESERLKSGFGDMLWFAISVVFNAHREEVGGGLARLV 645

Query: 541 LGPWLFVILVVTSSFTASLTSMMTVSRFSPSVVDIETLRQTNATVGCNFHSFIMRYLTNV 600
           L PWLFVILVVTSSFTASLTSMMTVSRF+PSVVDIETLRQTNATVGCNFHSFIMRYL NV
Sbjct: 646 LAPWLFVILVVTSSFTASLTSMMTVSRFAPSVVDIETLRQTNATVGCNFHSFIMRYLNNV 705

Query: 601 LHIPLDNIKTPVGIDDYPK 610
           L I  DNIKT   IDDYPK
Sbjct: 706 LQISPDNIKTLSSIDDYPK 709

BLAST of Lag0004282 vs. ExPASy TrEMBL
Match: A0A6J1KDI5 (glutamate receptor 2.5-like isoform X2 OS=Cucurbita maxima OX=3661 GN=LOC111492269 PE=3 SV=1)

HSP 1 Score: 879.8 bits (2272), Expect = 6.6e-252
Identity = 456/619 (73.67%), Postives = 520/619 (84.01%), Query Frame = 0

Query: 1   MHLIPKFDKSNEDIATISLPTAAFVPPLMSDQLHPPSFIQMAHDIKYHIQCIASIVGHFQ 60
           M  I + +K++ DIATISLP AA VPPL    L  PSFIQMAH I +H+QC A+IVGHFQ
Sbjct: 106 MQFISEINKTSVDIATISLPVAASVPPL----LPLPSFIQMAHHITFHMQCAAAIVGHFQ 165

Query: 61  WHKVTIIYENKNDMSVNNLETLTLLSNELQVFNAEIEQISEFSSSHSEATIEEKLKSLVG 120
           WHKVT+IYEN+NDMS+ N+E  TLLSNEL+VFNAEIEQIS FSSSH+EA IEEKLKSL+G
Sbjct: 166 WHKVTVIYENRNDMSI-NMEAFTLLSNELRVFNAEIEQISAFSSSHTEAMIEEKLKSLMG 225

Query: 121 HERNNVFIVLQFSIELAKLLFHKANQMNMMENGFVWIVGDEISSLVDSLDSSTLYNMQGV 180
            +RN VFIV+QFSIELAKLLFH+A +MNMM+NGFVWIVGDEISSL+DSLDSS  YNMQ V
Sbjct: 226 RDRNRVFIVVQFSIELAKLLFHRAKRMNMMDNGFVWIVGDEISSLMDSLDSSNFYNMQDV 285

Query: 181 IGYRTYFDHSKDSIKQFKSKFRRIYLSKYQ--VQEEDEEIKNGEPSIFALRAYDATLAVT 240
           IG+RTYF+HSKDS K+F+ KFRR+Y  +YQ   +EE+EE K+ EPSIFALRAYDA+ AV 
Sbjct: 286 IGFRTYFNHSKDSFKKFRRKFRRMYRLEYQHHDEEEEEEEKSAEPSIFALRAYDASWAVA 345

Query: 241 IAMRRLQGIMFSSEQFLQEVLAIEFEGLSGKIGFENGVLMQPPTFEIIYVVGKSYKEMGF 300
            AM +LQG  F+++Q L+++LA EFEGLSG IGFENG L QPPTFEIIYVVGKSYKEMGF
Sbjct: 346 AAMNKLQG-NFTNKQLLKQILATEFEGLSGSIGFENGTLKQPPTFEIIYVVGKSYKEMGF 405

Query: 301 WRQKVGFLFNNLMEDEVSIIISGRGRKGLLDLSNW--------GAPKRRINLDYSNSKLD 360
           WRQKVGF FN+LMEDE         + G+L+   +           KRR+NLD SNS+  
Sbjct: 406 WRQKVGF-FNSLMEDE-------EIKNGVLEFPRFVFWEGNEQTGLKRRMNLD-SNSEEH 465

Query: 361 RALKIGVPANSTFREFVKVSYHHINGIYISGFSISVFEAVVKNLPYPLSYQLVPFNGSYD 420
           R LKIGVPAN+TF EFVKVSY HING+YISG+SISVFEAVVKNLPYPL YQLVPFNGSYD
Sbjct: 466 RILKIGVPANNTFHEFVKVSYDHINGVYISGYSISVFEAVVKNLPYPLHYQLVPFNGSYD 525

Query: 421 GLVKQVYEKGLDAAVGDIGIYADRFQYVDFSEPYLVSGLMMIVKEEKENWKEIWIFLKTF 480
            LVKQV+ KGLDAAVGDIGI+ADRFQYVDF+E Y+VSGL+MIVKEEK NWKEIW+F+KTF
Sbjct: 526 ELVKQVHAKGLDAAVGDIGIFADRFQYVDFTESYMVSGLLMIVKEEKRNWKEIWVFMKTF 585

Query: 481 TMKMWIILPLSHIFIISIVWFVKEDSEELRSGFGNMLWFGISVIFYAQREQVNGGLARLV 540
           T  MWIILP+SH+FIIS+VWFV+ +SE L+SGFG+MLWF ISV+F A RE+V GGLARLV
Sbjct: 586 TTTMWIILPMSHMFIISVVWFVRPESERLKSGFGDMLWFAISVVFNAHREEVGGGLARLV 645

Query: 541 LGPWLFVILVVTSSFTASLTSMMTVSRFSPSVVDIETLRQTNATVGCNFHSFIMRYLTNV 600
           L PWLFVILVVTSSFTASLTSMMTVSRF+PSVVDIETLRQTNATVGCNFHSFIMRYL NV
Sbjct: 646 LAPWLFVILVVTSSFTASLTSMMTVSRFAPSVVDIETLRQTNATVGCNFHSFIMRYLNNV 705

Query: 601 LHIPLDNIKTPVGIDDYPK 610
           L I  DNIKT   IDDYPK
Sbjct: 706 LQISPDNIKTLSSIDDYPK 709

BLAST of Lag0004282 vs. ExPASy TrEMBL
Match: A0A6J1HCI9 (glutamate receptor 2.5-like isoform X1 OS=Cucurbita moschata OX=3662 GN=LOC111462745 PE=3 SV=1)

HSP 1 Score: 878.6 bits (2269), Expect = 1.5e-251
Identity = 456/619 (73.67%), Postives = 519/619 (83.84%), Query Frame = 0

Query: 1   MHLIPKFDKSNEDIATISLPTAAFVPPLMSDQLHPPSFIQMAHDIKYHIQCIASIVGHFQ 60
           M LI + +K++ DIA ISLP AA VPPL    L PPSFIQMAH I +H+QC A++VGHFQ
Sbjct: 106 MQLISEINKTSVDIAIISLPVAASVPPL----LPPPSFIQMAHHITFHMQCAAALVGHFQ 165

Query: 61  WHKVTIIYENKNDMSVNNLETLTLLSNELQVFNAEIEQISEFSSSHSEATIEEKLKSLVG 120
           WHKVT+IYEN+NDM + N+E LTLLSNEL+VFNAEIEQIS FSSSH+EA IEEKLKSL+G
Sbjct: 166 WHKVTVIYENRNDMWI-NMEALTLLSNELRVFNAEIEQISAFSSSHTEAVIEEKLKSLMG 225

Query: 121 HERNNVFIVLQFSIELAKLLFHKANQMNMMENGFVWIVGDEISSLVDSLDSSTLYNMQGV 180
            +RN VFIV+QFSIELAKLLFH+A +MNMM+NGFVWIVGDEISSL+DS DSS  YNMQGV
Sbjct: 226 RDRNRVFIVVQFSIELAKLLFHRAKRMNMMDNGFVWIVGDEISSLMDSSDSSHFYNMQGV 285

Query: 181 IGYRTYFDHSKDSIKQFKSKFRRIYLSKY--QVQEEDEEIKNGEPSIFALRAYDATLAVT 240
           IG+RTYF+HSKDS K+F+ KFRR+Y  +Y    +EE+EE K+ EPSIFALRAYDA+ AV 
Sbjct: 286 IGFRTYFNHSKDSFKKFRRKFRRMYRLEYHHHDEEEEEEEKSAEPSIFALRAYDASWAVA 345

Query: 241 IAMRRLQGIMFSSEQFLQEVLAIEFEGLSGKIGFENGVLMQPPTFEIIYVVGKSYKEMGF 300
            AM +LQG  FS++Q L+++LA EFEGLSG IGFENG L QPPTFEIIYVVGKSYKEMGF
Sbjct: 346 AAMDKLQG-NFSNKQLLKQILATEFEGLSGSIGFENGTLKQPPTFEIIYVVGKSYKEMGF 405

Query: 301 WRQKVGFLFNNLMEDEVSIIISGRGRKGLLDLSNW--------GAPKRRINLDYSNSKLD 360
           WRQKVGF FN+LMEDE         R G+L+   +           KRR+NLD SNS++ 
Sbjct: 406 WRQKVGF-FNSLMEDE-------EIRNGVLEFPRFVFWEGNEQTGLKRRMNLD-SNSEVP 465

Query: 361 RALKIGVPANSTFREFVKVSYHHINGIYISGFSISVFEAVVKNLPYPLSYQLVPFNGSYD 420
           R LKIGVPAN+TF EFVKVSY HING+YISG+SISVFEAVVKNLPYPL Y LVPFNGSYD
Sbjct: 466 RILKIGVPANNTFHEFVKVSYDHINGVYISGYSISVFEAVVKNLPYPLHYHLVPFNGSYD 525

Query: 421 GLVKQVYEKGLDAAVGDIGIYADRFQYVDFSEPYLVSGLMMIVKEEKENWKEIWIFLKTF 480
            LVKQV+ KGLDAAVGDIGI+ADRFQYVDF+E Y+VSGL+MIVKEEK NWKEIW+F+KTF
Sbjct: 526 ELVKQVHPKGLDAAVGDIGIFADRFQYVDFTESYMVSGLLMIVKEEKRNWKEIWVFMKTF 585

Query: 481 TMKMWIILPLSHIFIISIVWFVKEDSEELRSGFGNMLWFGISVIFYAQREQVNGGLARLV 540
           T  MWIILPLSH+FIIS+VWFV+ +SE L+SG G+MLWF ISV+F A RE+V GGLARLV
Sbjct: 586 TTTMWIILPLSHMFIISVVWFVRPESERLKSGIGDMLWFAISVVFNAHREEVGGGLARLV 645

Query: 541 LGPWLFVILVVTSSFTASLTSMMTVSRFSPSVVDIETLRQTNATVGCNFHSFIMRYLTNV 600
           L PWLFVILVVTSSFTASLTSMMTVSRF+PSVVDIETLRQTNATVGCNFHSFIMRYL NV
Sbjct: 646 LAPWLFVILVVTSSFTASLTSMMTVSRFAPSVVDIETLRQTNATVGCNFHSFIMRYLNNV 705

Query: 601 LHIPLDNIKTPVGIDDYPK 610
           L I  DNIKT   IDDYPK
Sbjct: 706 LQISPDNIKTLSSIDDYPK 709

BLAST of Lag0004282 vs. ExPASy TrEMBL
Match: A0A6J1HE83 (glutamate receptor 2.5-like isoform X3 OS=Cucurbita moschata OX=3662 GN=LOC111462745 PE=3 SV=1)

HSP 1 Score: 878.6 bits (2269), Expect = 1.5e-251
Identity = 456/619 (73.67%), Postives = 519/619 (83.84%), Query Frame = 0

Query: 1   MHLIPKFDKSNEDIATISLPTAAFVPPLMSDQLHPPSFIQMAHDIKYHIQCIASIVGHFQ 60
           M LI + +K++ DIA ISLP AA VPPL    L PPSFIQMAH I +H+QC A++VGHFQ
Sbjct: 106 MQLISEINKTSVDIAIISLPVAASVPPL----LPPPSFIQMAHHITFHMQCAAALVGHFQ 165

Query: 61  WHKVTIIYENKNDMSVNNLETLTLLSNELQVFNAEIEQISEFSSSHSEATIEEKLKSLVG 120
           WHKVT+IYEN+NDM + N+E LTLLSNEL+VFNAEIEQIS FSSSH+EA IEEKLKSL+G
Sbjct: 166 WHKVTVIYENRNDMWI-NMEALTLLSNELRVFNAEIEQISAFSSSHTEAVIEEKLKSLMG 225

Query: 121 HERNNVFIVLQFSIELAKLLFHKANQMNMMENGFVWIVGDEISSLVDSLDSSTLYNMQGV 180
            +RN VFIV+QFSIELAKLLFH+A +MNMM+NGFVWIVGDEISSL+DS DSS  YNMQGV
Sbjct: 226 RDRNRVFIVVQFSIELAKLLFHRAKRMNMMDNGFVWIVGDEISSLMDSSDSSHFYNMQGV 285

Query: 181 IGYRTYFDHSKDSIKQFKSKFRRIYLSKY--QVQEEDEEIKNGEPSIFALRAYDATLAVT 240
           IG+RTYF+HSKDS K+F+ KFRR+Y  +Y    +EE+EE K+ EPSIFALRAYDA+ AV 
Sbjct: 286 IGFRTYFNHSKDSFKKFRRKFRRMYRLEYHHHDEEEEEEEKSAEPSIFALRAYDASWAVA 345

Query: 241 IAMRRLQGIMFSSEQFLQEVLAIEFEGLSGKIGFENGVLMQPPTFEIIYVVGKSYKEMGF 300
            AM +LQG  FS++Q L+++LA EFEGLSG IGFENG L QPPTFEIIYVVGKSYKEMGF
Sbjct: 346 AAMDKLQG-NFSNKQLLKQILATEFEGLSGSIGFENGTLKQPPTFEIIYVVGKSYKEMGF 405

Query: 301 WRQKVGFLFNNLMEDEVSIIISGRGRKGLLDLSNW--------GAPKRRINLDYSNSKLD 360
           WRQKVGF FN+LMEDE         R G+L+   +           KRR+NLD SNS++ 
Sbjct: 406 WRQKVGF-FNSLMEDE-------EIRNGVLEFPRFVFWEGNEQTGLKRRMNLD-SNSEVP 465

Query: 361 RALKIGVPANSTFREFVKVSYHHINGIYISGFSISVFEAVVKNLPYPLSYQLVPFNGSYD 420
           R LKIGVPAN+TF EFVKVSY HING+YISG+SISVFEAVVKNLPYPL Y LVPFNGSYD
Sbjct: 466 RILKIGVPANNTFHEFVKVSYDHINGVYISGYSISVFEAVVKNLPYPLHYHLVPFNGSYD 525

Query: 421 GLVKQVYEKGLDAAVGDIGIYADRFQYVDFSEPYLVSGLMMIVKEEKENWKEIWIFLKTF 480
            LVKQV+ KGLDAAVGDIGI+ADRFQYVDF+E Y+VSGL+MIVKEEK NWKEIW+F+KTF
Sbjct: 526 ELVKQVHPKGLDAAVGDIGIFADRFQYVDFTESYMVSGLLMIVKEEKRNWKEIWVFMKTF 585

Query: 481 TMKMWIILPLSHIFIISIVWFVKEDSEELRSGFGNMLWFGISVIFYAQREQVNGGLARLV 540
           T  MWIILPLSH+FIIS+VWFV+ +SE L+SG G+MLWF ISV+F A RE+V GGLARLV
Sbjct: 586 TTTMWIILPLSHMFIISVVWFVRPESERLKSGIGDMLWFAISVVFNAHREEVGGGLARLV 645

Query: 541 LGPWLFVILVVTSSFTASLTSMMTVSRFSPSVVDIETLRQTNATVGCNFHSFIMRYLTNV 600
           L PWLFVILVVTSSFTASLTSMMTVSRF+PSVVDIETLRQTNATVGCNFHSFIMRYL NV
Sbjct: 646 LAPWLFVILVVTSSFTASLTSMMTVSRFAPSVVDIETLRQTNATVGCNFHSFIMRYLNNV 705

Query: 601 LHIPLDNIKTPVGIDDYPK 610
           L I  DNIKT   IDDYPK
Sbjct: 706 LQISPDNIKTLSSIDDYPK 709

BLAST of Lag0004282 vs. TAIR 10
Match: AT5G11210.1 (glutamate receptor 2.5 )

HSP 1 Score: 251.5 bits (641), Expect = 1.7e-66
Identity = 181/600 (30.17%), Postives = 297/600 (49.50%), Query Frame = 0

Query: 27  PLMSDQLHPPSFIQMAHDIKYHIQCIASIVGHFQWHKVTIIYENKNDMSVNNLETLTLLS 86
           PL+ D L  P FI+  HD    +Q I++I+  F+W +V  IY + N+     L  L    
Sbjct: 62  PLL-DSLRSPYFIRATHDDSSQVQAISAIIESFRWREVVPIYVD-NEFGEGILPNLV--- 121

Query: 87  NELQVFNAEIEQISEFSSSHSEATIEEKLKSLVGHERNNVFIVLQFSIELAKLLFHKANQ 146
           +  Q  N  I   S  S  +S+  I+++L  L+      VFIV     +L   LF  A +
Sbjct: 122 DAFQEINVRIRYRSAISLHYSDDQIKKELYKLMTMP-TRVFIVHMLP-DLGSRLFSIAKE 181

Query: 147 MNMMENGFVWIVGDEISSLVDSLDSSTLYNMQGVIGYRTYFDHSKDSIKQFKSKFRRIYL 206
           ++M+  G+VWIV + I+ L+  +  S+L NM GV+G +TYF  SK+ +            
Sbjct: 182 IDMLSKGYVWIVTNGIADLMSIMGESSLVNMHGVLGVKTYFAKSKELLH---------LE 241

Query: 207 SKYQVQEEDEEIKNGEPSIFALRAYDATLAVTIAMRRLQ--------------------- 266
           +++Q +   EE+ N     FA  AYDA  A+ +++  ++                     
Sbjct: 242 ARWQKRFGGEELNN-----FACWAYDAATALAMSVEEIRHVNMSFNTTKEDTSRDDIGTD 301

Query: 267 ----GIMFSSEQFLQEVLAIEFEGLSGKIGFENGVLMQPPTFEIIYVVGKSYKEMGFWRQ 326
               G+  S  + L  +  + F+G++G+   +NG L +  TF+II +     + +GFW+ 
Sbjct: 302 LDELGVALSGPKLLDALSTVSFKGVAGRFQLKNGKL-EATTFKIINIEESGERTVGFWKS 361

Query: 327 KVGFLFNNLMEDEVSIIISGRGRKGLLDLSNWGAPKRRINLDYSNSKLDRALKIGVPANS 386
           KVG L  +L  D+VS   S R  + ++    W      +   +      + L+I VP   
Sbjct: 362 KVG-LVKSLRVDKVS--HSSRRLRPII----WPGDTIFVPKGWEFPTNAKKLRIAVPKKD 421

Query: 387 TFREFVKVSY-HHINGIYISGFSISVFEAVVKNLPYPLSYQLVPFN-------GSYDGLV 446
            F  FV+V+   + N   ++GF I VF  V+  +PY +SY+ +PF+       GSYD +V
Sbjct: 422 GFNNFVEVTKDENTNVPTVTGFCIDVFNTVMSQMPYAVSYEYIPFDTPDGKPRGSYDEMV 481

Query: 447 KQVYEKGLDAAVGDIGIYADRFQYVDFSEPYLVSGLMMIVKEEKENWKEIWIFLKTFTMK 506
             V+    D AVGD  I A+R  YVDF+ PY  +G++ +V  +    K  W+FLK  T +
Sbjct: 482 YNVFLGEFDGAVGDTTILANRSHYVDFALPYSETGIVFLVPVKDGKEKGEWVFLKPLTKE 541

Query: 507 MWIILPLSHIFIISIVW-FVKEDSEELR-----SGFGNMLWFGISVIFYAQREQVNGGLA 566
           +W++   S ++I  +VW F  +  EE R         ++ +F  S +F+A R        
Sbjct: 542 LWLVTAASFLYIGIMVWIFEYQADEEFREQMIIDKISSVFYFSFSTLFFAHRRPSESFFT 601

Query: 567 RLVLGPWLFVILVVTSSFTASLTSMMTVSRFSPSVVDIETLRQTNATVGCNFHSFIMRYL 588
           R+++  W FV+L++T S+TA+LTSM+TV    P+V  ++ LR++   +G    SF    L
Sbjct: 602 RVLVVVWCFVLLILTQSYTATLTSMLTVQELRPTVRHMDDLRKSGVNIGYQTGSFTFERL 632

BLAST of Lag0004282 vs. TAIR 10
Match: AT5G27100.1 (glutamate receptor 2.1 )

HSP 1 Score: 242.3 bits (617), Expect = 1.0e-63
Identity = 186/610 (30.49%), Postives = 298/610 (48.85%), Query Frame = 0

Query: 3   LIPKFDKSNEDIATISLPTAAFVPPLMSDQLHPPSFIQMAHDIKYHIQCIASIVGHFQWH 62
           +I    KS   I T S    A  P L S  +    F +  +D    +  I  I+  F W 
Sbjct: 112 MIEMGQKSQVPIVTYS----ATSPSLAS--IRSQYFFRATYDDSSQVHAIKEIIKLFGWR 171

Query: 63  KVTIIYENKNDMSVNNLETLTLLSNELQVFNAEIEQISEFSSSHSEATIEEKLKSLVGHE 122
           +V  +Y +           +  L++ LQ  N  I   +  S + ++  I  +L  ++   
Sbjct: 172 EVAPVYVD----DTFGEGIMPRLTDVLQEINVRIPYRTVISPNATDDEISVELLRMMTLP 231

Query: 123 RNNVFIVLQFSIELAKLLFHKANQMNMMENGFVWIVGDEISSLVDSLDSSTLYNMQGVIG 182
              VF+V    + LA   F KA ++ +M+ G+VWI+ + I+ ++  ++ + +  MQGV+G
Sbjct: 232 -TRVFVVHLVEL-LASRFFAKATEIGLMKQGYVWILTNTITDVLSIMNETEIETMQGVLG 291

Query: 183 YRTYFDHSKDSIKQFKSKFRRIYLSKYQVQEEDEEIKNGEPSIFALRAYDATLAVTIAMR 242
            +TY   SK+ ++ F+S++ +                  + +++ L AYDAT A+ +A+ 
Sbjct: 292 VKTYVPRSKE-LENFRSRWTK-------------RFPISDLNVYGLWAYDATTALALAIE 351

Query: 243 -------------------RLQGIMFS--SEQFLQEVLAIEFEGLSGKIGFENGVLMQPP 302
                               LQG+  S    + LQ +  + F+GL+G   F NG L QP 
Sbjct: 352 EAGTSNLTFVKMDAKRNVSELQGLGVSQYGPKLLQTLSRVRFQGLAGDFQFINGEL-QPS 411

Query: 303 TFEIIYVVGKSYKEMGFWRQKVGFLFNNLMEDEVSIIISGRGRKGLLDLSNWGAPKRRIN 362
            FEI+ V G+  + +GFW ++ G LF N+ +   S       +  L  +  W      + 
Sbjct: 412 VFEIVNVNGQGGRTIGFWMKEYG-LFKNVDQKPASKTTFSSWQDRLRPII-WPGDTTSVP 471

Query: 363 LDYSNSKLDRALKIGVPANSTFREFVKVSYHHI-NGIYISGFSISVFEAVVKNLPYPLSY 422
             +      + L+IGVP N+TF++FVK +   I N    SGFSI  FEAV++ +PY +SY
Sbjct: 472 KGWEIPTNGKRLQIGVPVNNTFQQFVKATRDPITNSTIFSGFSIDYFEAVIQAIPYDISY 531

Query: 423 QLVPF-NGSYDGLVKQVYEKGLDAAVGDIGIYADRFQYVDFSEPYLVSGLMMIVKEEKEN 482
             +PF +G YD LV QVY    DA V D  I ++R  YVDFS PY  SG+ ++V  +   
Sbjct: 532 DFIPFQDGGYDALVYQVYLGKYDAVVADTTISSNRSMYVDFSLPYTPSGVGLVVPVKDSV 591

Query: 483 WKEIWIFLKTFTMKMWIILPLSHIFIISIVWFVKEDSEELRSGFG-----NMLWFGISVI 542
            +   IFL   T+ +W+I  LS   I  +VW ++        G G      + WF  S++
Sbjct: 592 RRSSTIFLMPLTLALWLISLLSFFIIGLVVWVLEHRVNPDFDGPGQYQLSTIFWFSFSIM 651

Query: 543 FYAQREQVNGGLARLVLGPWLFVILVVTSSFTASLTSMMTVSRFSPSVVDIETLRQTNAT 585
            +A RE+V    AR+V+  W F++LV+T S+TASL S++T     P+V +I +L     +
Sbjct: 652 VFAPRERVLSFWARVVVIIWYFLVLVLTQSYTASLASLLTTQHLHPTVTNINSLLAKGES 691

BLAST of Lag0004282 vs. TAIR 10
Match: AT2G24710.1 (glutamate receptor 2.3 )

HSP 1 Score: 235.7 bits (600), Expect = 9.7e-62
Identity = 166/587 (28.28%), Postives = 292/587 (49.74%), Query Frame = 0

Query: 33  LHPPSFIQMAHDIKYHIQCIASIVGHFQWHKVTIIYENKNDMSVNNLETLTLLSNELQVF 92
           L  P F++  ++  + +Q I +I+  F W +V  +Y + N      +  LT   + LQ  
Sbjct: 135 LRSPYFLRATYEDSFQVQPIKAIIKLFGWREVVPVYID-NTFGEGIMPRLT---DALQDI 194

Query: 93  NAEIEQISEFSSSHSEATIEEKLKSLVGHERNNVFIVLQFSIELAKLLFHKANQMNMMEN 152
           N  I   S  + + ++  I  +L  ++ +    VF+V  +  +LA   F KA ++ +ME 
Sbjct: 195 NVRIPYRSVIAINATDHEISVELLKMM-NMPTRVFLVHMY-YDLASRFFIKAKELGLMEP 254

Query: 153 GFVWIVGDEISSLVDSLDSSTLYNMQGVIGYRTYFDHSKDSIKQFKSKFRRIYLSKYQVQ 212
           G+VWI+ + +   +  ++ + +  M+GV+G +TY   S D +++F+S++R ++       
Sbjct: 255 GYVWILTNGVIDDLSLINETAVEAMEGVLGIKTYIPKSPD-LEKFRSRWRSLF------- 314

Query: 213 EEDEEIKNGEPSIFALRAYDATLAVTIAMR----------------------RLQGIMFS 272
                    E S++ L AYDAT A+ +A+                          G+   
Sbjct: 315 ------PRVELSVYGLWAYDATTALAVAIEEAGTNNMTFSKVVDTGRNVSELEALGLSQF 374

Query: 273 SEQFLQEVLAIEFEGLSGKIGFENGVLMQPPTFEIIYVVGKSYKEMGFWRQKVGFLFNNL 332
             + LQ +L ++F GL+G+  F  G L QP  FEI+ ++    K +GFW++  G +    
Sbjct: 375 GPKLLQTLLTVQFRGLAGEFRFFRGQL-QPSVFEIVNIINTGEKSIGFWKEGNGLV--KK 434

Query: 333 MEDEVSIIISGRGRKGLLDLSNWGAPKRRINLDYSNSKLDRALKIGVPANSTFREFVKVS 392
           ++ + S I +    K  L    W      +   +      + L+IGVP  + + + VKV+
Sbjct: 435 LDQQASSISALSTWKDHLKHIVWPGEADSVPKGWQIPTKGKKLRIGVPKRTGYTDLVKVT 494

Query: 393 YHHI-NGIYISGFSISVFEAVVKNLPYPLSYQLVPFN-------GSYDGLVKQVYEKGLD 452
              I N   ++GF I  FEAV++ LPY +SY+ +PF        G+Y+ LV QVY    D
Sbjct: 495 RDPITNSTVVTGFCIDFFEAVIRELPYDVSYEFIPFEKPDGKTAGNYNDLVYQVYLGRYD 554

Query: 453 AAVGDIGIYADRFQYVDFSEPYLVSGLMMIVKEEKENWKEIWIFLKTFTMKMWIILPLSH 512
           A VGD  I  +R  YVDF+ P++ SG+ +IV+      ++  +F+K  + K+W+   +S 
Sbjct: 555 AVVGDTTILVNRSSYVDFTFPFIKSGVGLIVEMTDPVKRDYILFMKPLSWKLWLTSFISF 614

Query: 513 IFIISIVWFVK-----EDSEELRSGFGNMLWFGISVIFYAQREQVNGGLARLVLGPWLFV 572
             +   VW ++     + S   R     + WF  S + +A RE+V    AR ++  W F+
Sbjct: 615 FLVGCTVWVLEYKRNPDFSGPPRFQASTICWFAFSTMVFAPRERVFSFWARALVIAWYFL 674

Query: 573 ILVVTSSFTASLTSMMTVSRFSPSVVDIETLRQTNATVGCNFHSFIM 585
           +LV+T S+TASL S++T  + +P++  + +L +   TVG    SFI+
Sbjct: 675 VLVLTQSYTASLASLLTSQKLNPTITSMSSLLEKGETVGYQRTSFIL 698

BLAST of Lag0004282 vs. TAIR 10
Match: AT2G29120.1 (glutamate receptor 2.7 )

HSP 1 Score: 234.2 bits (596), Expect = 2.8e-61
Identity = 179/603 (29.68%), Postives = 294/603 (48.76%), Query Frame = 0

Query: 19  LPTAAF--VPPLMSDQLHPPSFIQMAHDIKYHIQCIASIVGHFQWHKVTIIYENKNDMSV 78
           +PT  F    PL++  ++ P F++   D    ++ IA+IV  F W  V  IY + N+   
Sbjct: 128 VPTITFSATCPLLT-SINSPYFVRATLDDSSQVKAIAAIVKSFGWRNVVAIYVD-NEFGE 187

Query: 79  NNLETLTLLSNELQVFNAEIEQISEFSSSHSEATIEEKLKSLVGHERNNVFIVLQFSIEL 138
             L  LT    ++Q F      I +   ++ +  ++E  K +    R  VF+V      L
Sbjct: 188 GILPLLTDALQDVQAFVVNRCLIPQ--EANDDQILKELYKLMTMQTR--VFVV-HMPPTL 247

Query: 139 AKLLFHKANQMNMMENGFVWIVGDEISSLVDSLD-SSTLYNMQGVIGYRTYFDHSKDSIK 198
               F KA ++ MME G+VW++ D + +L+ S +  S+L NMQGV+G R++   SK  +K
Sbjct: 248 GFRFFQKAREIGMMEEGYVWLLTDGVMNLLKSNERGSSLENMQGVLGVRSHIPKSK-KLK 307

Query: 199 QFKSKFRRIYLSKYQVQEEDEEIKNGEPSIFALRAYDATLAVTIAMRRLQ---------- 258
            F+ ++ +++  K      DEE+     +IFALRAYD+  A+ +A+ +            
Sbjct: 308 NFRLRWEKMFPKK----GNDEEM-----NIFALRAYDSITALAMAVEKTNIKSLRYDHPI 367

Query: 259 ------------GIMFSSEQFLQEVLAIEFEGLSGKIGFENGVLMQPPTFEIIYVVGKSY 318
                       G+       L+ +  + F GL+G+    NG L +   F++I ++G   
Sbjct: 368 ASGNNKTNLGTLGVSRYGPSLLKALSNVRFNGLAGEFELINGQL-ESSVFDVINIIGSEE 427

Query: 319 KEMGFWRQKVGFLFNNLMEDEVSIIISGRGRKGLLDLSNWGAPKRRINLDYSNSKLDRAL 378
           + +G WR   G +  N      + ++  R     L    W    + +   +      + L
Sbjct: 428 RIIGLWRPSNGIV--NAKSKNTTSVLGER-----LGPVIWPGKSKDVPKGWQIPTNGKML 487

Query: 379 KIGVPANSTFREFVKVSYHHI-NGIYISGFSISVFEAVVKNLPYPLSYQLVPF---NGSY 438
           ++G+P    F EFV      I N +  +G+ I +FEAV+K LPY +  + + F   + +Y
Sbjct: 488 RVGIPVKKGFLEFVDAKIDPISNAMTPTGYCIEIFEAVLKKLPYSVIPKYIAFLSPDENY 547

Query: 439 DGLVKQVYEKGLDAAVGDIGIYADRFQYVDFSEPYLVSGLMMIVKEEKENWKEIWIFLKT 498
           D +V QVY    DA VGD+ I A+R  YVDF+ PY  SG+ M+V   K+N K  W+FL+ 
Sbjct: 548 DEMVYQVYTGAYDAVVGDVTIVANRSLYVDFTLPYTESGVSMMV-PLKDN-KNTWVFLRP 607

Query: 499 FTMKMWIILPLSHIFIISIVWFVKEDSEELRSG-----FGNMLWFGISVIFYAQREQVNG 558
           +++ +W+      +FI  IVW ++        G      G   WF  S + +A RE+V  
Sbjct: 608 WSLDLWVTTACFFVFIGFIVWILEHRVNTDFRGPPHHQIGTSFWFAFSTMNFAHREKVVS 667

Query: 559 GLARLVLGPWLFVILVVTSSFTASLTSMMTVSRFSPSVVDIETLRQTNATVGCNFHSFIM 588
            LAR V+  W FV+LV+  S+TA+LTS  TV    P+V + + L + N  +G    +F+ 
Sbjct: 668 NLARFVVLVWCFVVLVLIQSYTANLTSFFTVKLLQPTVTNWKDLIKFNKNIGYQRGTFVR 703

BLAST of Lag0004282 vs. TAIR 10
Match: AT2G24720.1 (glutamate receptor 2.2 )

HSP 1 Score: 223.0 bits (567), Expect = 6.5e-58
Identity = 177/598 (29.60%), Postives = 294/598 (49.16%), Query Frame = 0

Query: 22  AAFVPPLMSDQLHPPSFIQMAHDIKYHIQCIASIVGHFQWHKVTIIYENKNDMSVNNLET 81
           +A  P L S  L  P F +  ++    +  I +I+  F W +V  +Y + N      +  
Sbjct: 127 SATSPSLTS--LRSPYFFRATYEDSSQVHAIKAIIKLFGWREVVPVYID-NTFGEGIMPR 186

Query: 82  LTLLSNELQVFNAEIEQISEFSSSHSEATIEEKLKSLVGHERNNVFIVLQFSIELAKLLF 141
           LT   + LQ  N  I   S    + ++  I  +L  ++ +    VFIV   S  LA  +F
Sbjct: 187 LT---DSLQDINVRIPYRSVIPLNATDQDISVELLKMM-NMPTRVFIV-HMSSSLASTVF 246

Query: 142 HKANQMNMMENGFVWIVGDEISSLVDSLDSSTLYNMQGVIGYRTYFDHSKDSIKQFKSKF 201
            KA ++ +M+ G+VWI+ + +   + S++ + +  M+GV+G +TY   SKD ++ F+S++
Sbjct: 247 IKAKELGLMKPGYVWILTNGVMDGLRSINETGIEAMEGVLGIKTYIPKSKD-LETFRSRW 306

Query: 202 RRIYLSKYQVQEEDEEIKNGEPSIFALRAYDATLAVTIAMR------------------- 261
           +R                  E +++ L AYDAT A+ +A+                    
Sbjct: 307 KR-------------RFPQMELNVYGLWAYDATTALAMAIEDAGINNMTFSNVDTGKNVS 366

Query: 262 RLQGIMFS--SEQFLQEVLAIEFEGLSGKIGFENGVLMQPPTFEIIYVVGKSYKEMGFWR 321
            L G+  S    + LQ V  ++F+GL+G   F +G L QP  FEI+ ++G   + +GFW 
Sbjct: 367 ELDGLGLSQFGPKLLQTVSTVQFKGLAGDFHFVSGQL-QPSVFEIVNMIGTGERSIGFWT 426

Query: 322 QKVGFLFNNLMEDEVSIIISGRGRKGLLDLSNWGAPKRRINLDYSNSKLDRALKIGVPAN 381
           +  G L   L ++  SI         L  +  W      +   +      + L+IGVP  
Sbjct: 427 EGNG-LVKKLDQEPRSIGTLSTWPDHLKHII-WPGEAVSVPKGWEIPTNGKKLRIGVPKR 486

Query: 382 STFREFVKVSYHHI-NGIYISGFSISVFEAVVKNLPYPLSYQLVPFN-------GSYDGL 441
             F + VKV+   I N   + GF I  FEAV++ +PY +SY+  PF        G+++ L
Sbjct: 487 IGFTDLVKVTRDPITNSTVVKGFCIDFFEAVIQAMPYDVSYEFFPFEKPNGEPAGNHNDL 546

Query: 442 VKQVYEKGLDAAVGDIGIYADRFQYVDFSEPYLVSGLMMIVKEEKENWKEIWIFLKTFTM 501
           V QVY    DA VGD  I A+R  +VDF+ P++ SG+ +IV  + E  ++ + FLK  ++
Sbjct: 547 VHQVYLGQFDAVVGDTTILANRSSFVDFTLPFMKSGVGLIVPLKDEVKRDKFSFLKPLSI 606

Query: 502 KMWIILPLSHIFIISI-VWFVKEDSEELRSGFGN-----MLWFGISVIFYAQREQVNGGL 561
           ++W +  L   F++ I VW ++        G  N     + WF  S + +A RE+V    
Sbjct: 607 ELW-LTTLVFFFLVGISVWTLEHRVNSDFRGPANYQASTIFWFAFSTMVFAPRERVLSFG 666

Query: 562 ARLVLGPWLFVILVVTSSFTASLTSMMTVSRFSPSVVDIETLRQTNATVGCNFHSFIM 585
           AR ++  W FV+LV+T S+TASL S++T  + +P++  + +L     TVG    SFI+
Sbjct: 667 ARSLVVTWYFVLLVLTQSYTASLASLLTSQQLNPTITSMSSLLHRGETVGYQRTSFIL 698

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
XP_023546340.13.5e-25574.47glutamate receptor 2.5-like [Cucurbita pepo subsp. pepo][more]
KAG6598371.15.6e-25374.47Glutamate receptor 2.5, partial [Cucurbita argyrosperma subsp. sororia][more]
XP_022997324.11.4e-25173.67glutamate receptor 2.5-like isoform X3 [Cucurbita maxima][more]
XP_022997321.11.4e-25173.67glutamate receptor 2.5-like isoform X1 [Cucurbita maxima][more]
XP_022997323.11.4e-25173.67glutamate receptor 2.5-like isoform X2 [Cucurbita maxima][more]
Match NameE-valueIdentityDescription
Q9LFN52.4e-6530.17Glutamate receptor 2.5 OS=Arabidopsis thaliana OX=3702 GN=GLR2.5 PE=2 SV=2[more]
O046601.5e-6230.49Glutamate receptor 2.1 OS=Arabidopsis thaliana OX=3702 GN=GLR2.1 PE=2 SV=2[more]
Q9SHV21.4e-6028.28Glutamate receptor 2.3 OS=Arabidopsis thaliana OX=3702 GN=GLR2.3 PE=2 SV=1[more]
Q9LFN81.4e-6028.64Glutamate receptor 2.6 OS=Arabidopsis thaliana OX=3702 GN=GLR2.6 PE=2 SV=2[more]
Q8LGN04.0e-6029.68Glutamate receptor 2.7 OS=Arabidopsis thaliana OX=3702 GN=GLR2.7 PE=2 SV=3[more]
Match NameE-valueIdentityDescription
A0A6J1K7656.6e-25273.67glutamate receptor 2.5-like isoform X1 OS=Cucurbita maxima OX=3661 GN=LOC1114922... [more]
A0A6J1KB406.6e-25273.67glutamate receptor 2.5-like isoform X3 OS=Cucurbita maxima OX=3661 GN=LOC1114922... [more]
A0A6J1KDI56.6e-25273.67glutamate receptor 2.5-like isoform X2 OS=Cucurbita maxima OX=3661 GN=LOC1114922... [more]
A0A6J1HCI91.5e-25173.67glutamate receptor 2.5-like isoform X1 OS=Cucurbita moschata OX=3662 GN=LOC11146... [more]
A0A6J1HE831.5e-25173.67glutamate receptor 2.5-like isoform X3 OS=Cucurbita moschata OX=3662 GN=LOC11146... [more]
Match NameE-valueIdentityDescription
AT5G11210.11.7e-6630.17glutamate receptor 2.5 [more]
AT5G27100.11.0e-6330.49glutamate receptor 2.1 [more]
AT2G24710.19.7e-6228.28glutamate receptor 2.3 [more]
AT2G29120.12.8e-6129.68glutamate receptor 2.7 [more]
AT2G24720.16.5e-5829.60glutamate receptor 2.2 [more]
InterPro
Analysis Name: InterPro Annotations of Sponge gourd (AG-4) v1
Date Performed: 2022-08-01
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR001320Ionotropic glutamate receptorSMARTSM00079GluR_14coord: 352..560
e-value: 0.0038
score: -66.9
IPR001320Ionotropic glutamate receptorPFAMPF00060Lig_chancoord: 463..572
e-value: 2.1E-13
score: 50.4
NoneNo IPR availableGENE3D1.10.287.70coord: 465..593
e-value: 2.5E-15
score: 58.4
NoneNo IPR availableGENE3D3.40.50.2300coord: 45..221
e-value: 1.8E-29
score: 104.6
NoneNo IPR availableGENE3D3.40.190.10coord: 350..464
e-value: 3.0E-15
score: 58.2
NoneNo IPR availablePANTHERPTHR18966IONOTROPIC GLUTAMATE RECEPTORcoord: 4..609
NoneNo IPR availablePANTHERPTHR18966:SF401GLUTAMATE RECEPTORcoord: 4..609
NoneNo IPR availableSUPERFAMILY53850Periplasmic binding protein-like IIcoord: 351..592
IPR001638Solute-binding protein family 3/N-terminal domain of MltFPFAMPF00497SBP_bac_3coord: 379..458
e-value: 2.7E-11
score: 43.3
IPR001828Receptor, ligand binding regionPFAMPF01094ANF_receptorcoord: 21..288
e-value: 1.1E-27
score: 97.1
IPR028082Periplasmic binding protein-like ISUPERFAMILY53822Periplasmic binding protein-like Icoord: 22..300

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Lag0004282.1Lag0004282.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0007623 circadian rhythm
biological_process GO:0034220 ion transmembrane transport
biological_process GO:0006355 regulation of transcription, DNA-templated
cellular_component GO:0016021 integral component of membrane
cellular_component GO:0005634 nucleus
cellular_component GO:0016020 membrane
molecular_function GO:0015276 ligand-gated ion channel activity
molecular_function GO:0000976 transcription cis-regulatory region binding
molecular_function GO:0008270 zinc ion binding