Lag0004272 (gene) Sponge gourd (AG‐4) v1

BLAST of Lag0004272 vs. NCBI nr
Match: XP_022962252.1 (glutamate receptor 2.5-like [Cucurbita moschata])

HSP 1 Score: 948.0 bits (2449), Expect = 5.3e-272
Identity = 536/845 (63.43%), Postives = 629/845 (74.44%), Query Frame = 0

Query: 1   MIRNIF----CFLGLLFLLLLLSSEAHTSKKEVNCQKTHRNESTRIGVVFDSGSQIGKQQ 60
           ++RN F    CFLGLL LLLL S   H  + + N      N++TRIG VFDSGSQIGKQQ
Sbjct: 2   ILRNGFCRPSCFLGLLLLLLLSSEGLHCQRNQPN------NKTTRIGAVFDSGSQIGKQQ 61

Query: 61  MIAMKMALRRFRL-SSCVKLELRLHDSHPNFSSPTSSASQN------------------- 120
           M+AMKM LR F L SSC KLEL LHDSHPNF+S       N                   
Sbjct: 62  MVAMKMGLRHFHLSSSCHKLELLLHDSHPNFTSSALDLITNGGVKAVVIGSVRAQDLIAI 121

Query: 121 ---------PIFDSNFQQHHP-------------PNAMHCFNS--DPFSMAAKVTVFHEI 180
                    PI   +  Q HP              + M C  S    F +  KVTVF+EI
Sbjct: 122 SDHEIPVGVPILSISATQLHPKKIPSLIQMANNITHRMQCIVSILTHFQLPPKVTVFYEI 181

Query: 181 N--------SFVSIRRLFDSFRSANIEIEHFLALSYSSSNQAEILIEKELEKFMNSQRNN 240
                    S +S+ RLFDSFR  N+EI+H LALS SSSNQAEILIE EL+  M SQRN 
Sbjct: 182 TNIDHPSEYSSISVDRLFDSFRLVNVEIDHRLALS-SSSNQAEILIENELKSAMKSQRNG 241

Query: 241 RVFIVTQLSLKLVRLLFTKAKNMNMVGNGYAWIVSSDVFDHVVSLD-SPSLLNKMEGVIG 300
            VF+VTQLSL+L  LLFTKAK +NMVGNGY WIVS DV D + SLD S SLL KMEGVIG
Sbjct: 242 -VFVVTQLSLELADLLFTKAKKLNMVGNGYTWIVSDDVLDLIPSLDSSSSLLYKMEGVIG 301

Query: 301 FRTYFDDTKKSFKSFETKLEKMYSLEYPQDKEPIKASIFTVRAYDASWAISKAIEKLGEK 360
           FRTYF+DTKKSFKSFETK +KMY+LEYP+DKEPIKASIF VRAYD   +I++A++ LG+ 
Sbjct: 302 FRTYFNDTKKSFKSFETKFKKMYNLEYPEDKEPIKASIFAVRAYDVVRSIARAMKTLGDN 361

Query: 361 --FSSHQLMEKILESNFEGLSGMVRFKNGMLISQAPTFQILKVVDGSLKDVAFWTPKLGF 420
              SS QL+E ILESNFEGLSGMVRFKNGMLIS++P F+I+KVVD S K VAFWTPK GF
Sbjct: 362 NLSSSDQLLETILESNFEGLSGMVRFKNGMLISRSPNFEIIKVVDQSYKPVAFWTPKSGF 421

Query: 421 SESLVANNKELEF----MENVAAVRHLSQTFSRELDGYGEIRRLKFAVPNQGACREFVNV 480
           +ES V NNK+       M N+  VRHLS++F+RE DG GE ++L FAVP QGAC+EFVNV
Sbjct: 422 AESFVENNKKSALRSNNMGNMVGVRHLSESFTRE-DGDGE-KQLTFAVPGQGACQEFVNV 481

Query: 481 NYHLNGTINNFTGFSIDLFKAVMHNI--TMSYHFVPFHKTYNEMIDAVYNKTYDGAVGDI 540
           +   NGT  +F+GFSI +F  +M+NI    SY F  F+ +YN+MIDAVY K YDGAVGDI
Sbjct: 482 HTFENGT-EHFSGFSIAVFNEIMNNIKNMPSYKFARFNHSYNDMIDAVYVKEYDGAVGDI 541

Query: 541 TILAERFRRVDFTVAYLKTDIVMVVTEKEEKWKKWWAFMEAFDFEVWLLIPTMHLFVSSI 600
           TILA+RF++VDFTVAYLKTDIVMVV EK E+W+K WAFM+AF  +VW+LIPTMHLF+SS+
Sbjct: 542 TILAKRFQQVDFTVAYLKTDIVMVVREKHERWRKLWAFMDAFKPQVWVLIPTMHLFISSL 601

Query: 601 IWLIERQNNDELKGFGNMLWFSISIIFYIHREPVKNGLARLVLGPWLFAILVMTTSFSAS 660
           IWLIER+NN+ELKGFGNMLWFS+S+IFY+ REPVKNGLARLVLGPWLFAI V+T SFSAS
Sbjct: 602 IWLIERENNEELKGFGNMLWFSVSLIFYMQREPVKNGLARLVLGPWLFAIFVVTASFSAS 661

Query: 661 LTSMMTISWSQPSVLDVETLKKMNATVGCNAESFIYNFLKNTLKFEPSKIVKMNSIDDYP 720
           LTSM+TISWSQPSV  VE LK+MNATVGCNAESFI N+LK+TL+FE S I +M S+D+YP
Sbjct: 662 LTSMLTISWSQPSVTTVEMLKEMNATVGCNAESFICNYLKDTLQFESSNIKRMESLDNYP 721

Query: 721 EAFKNDTIKAAFFISPHAEVFLAKNCRGYTKAVSSFKLGGIGFAFQKGSALAAMVSESIA 780
           +AF+++TIKAAFFISPHA+VFLAKNCRGYTK VSSFKLGGIGFAF KGS  AA VS+SIA
Sbjct: 722 KAFEDNTIKAAFFISPHADVFLAKNCRGYTKGVSSFKLGGIGFAFPKGSDFAAKVSKSIA 781

BLAST of Lag0004272 vs. NCBI nr
Match: XP_023546339.1 (glutamate receptor 2.5-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 938.3 bits (2424), Expect = 4.2e-269
Identity = 538/851 (63.22%), Postives = 631/851 (74.15%), Query Frame = 0

Query: 1   MIRNIF----CFLGLLFLLLLLSSEAHTSKKEVNCQKTHRNESTRIGVVFDSGSQIGKQQ 60
           ++RN F    CFLGLL LLLL S   H    + N      N++TRIG VFDSGSQIGKQQ
Sbjct: 2   ILRNGFCRPSCFLGLLLLLLLRSEGLHCQIHQPN------NKTTRIGAVFDSGSQIGKQQ 61

Query: 61  MIAMKMALRRFRL-SSCVKLELRLHDSHPNFSS-----PT----------------SSAS 120
           M+AMKM LR F L SSC KLEL LHDSHPNF+S     PT                S  +
Sbjct: 62  MVAMKMGLRHFHLSSSCHKLELLLHDSHPNFTSSVTVFPTALDLITNGGVKAVVIGSVRA 121

Query: 121 QN-------------PIFDSNFQQHHP-------------PNAMHCFNS--DPFSMAAKV 180
           Q+             PI   +  Q HP              + M C  S    F +  KV
Sbjct: 122 QDLIAISDHEIPVGVPILSISATQLHPKKIPSLIQMANNITHRMQCIVSILTHFQLPPKV 181

Query: 181 TVFHEIN--------SFVSIRRLFDSFRSANIEIEHFLALSYSSSNQAEILIEKELEKFM 240
           TVF+EI         S +S  RLFDSFRS N+EI+H LALS SSSNQAEILIE EL++ M
Sbjct: 182 TVFYEITNIDHPSEYSSISADRLFDSFRSVNVEIDHRLALS-SSSNQAEILIENELKRAM 241

Query: 241 NSQRNNRVFIVTQLSLKLVRLLFTKAKNMNMVGNGYAWIVSSDVFDHVVSLD-SPSLLNK 300
            SQRN  VF+VTQLSL+L  LLFTKAK +NMVGNGY WIVS DV D + SLD S SLL K
Sbjct: 242 KSQRNG-VFVVTQLSLELADLLFTKAKKLNMVGNGYTWIVSDDVLDLIPSLDSSSSLLYK 301

Query: 301 MEGVIGFRTYFDDTKKSFKSFETKLEKMYSLEYPQDKEPIKASIFTVRAYDASWAISKAI 360
           MEGVIGFRTYF DTKKSFKSFETK +KMY+LEYP+DKEPIKASIF VRAYD   +I++A+
Sbjct: 302 MEGVIGFRTYFSDTKKSFKSFETKFKKMYNLEYPEDKEPIKASIFAVRAYDVVRSIARAM 361

Query: 361 EKLGEK--FSSHQLMEKILESNFEGLSGMVRFKNGMLISQAPTFQILKVVDGSLKDVAFW 420
           + LG+    SS QL+E ILESNFEGLSGMVRFKNGMLISQ+P F+I+KVVD S K VAFW
Sbjct: 362 KTLGDNNLSSSDQLLETILESNFEGLSGMVRFKNGMLISQSPNFEIIKVVDQSYKPVAFW 421

Query: 421 TPKLGFSESLVANNKELEF----MENVAAVRHLSQTFSRELDGYGEIRRLKFAVPNQGAC 480
           TPK GF+ES V NNK+       M N+  VRHLS++F+RE DG GE ++L FAVP QGAC
Sbjct: 422 TPKSGFAESFVENNKKSALRSNNMGNMVGVRHLSESFTRE-DGDGE-KQLTFAVPGQGAC 481

Query: 481 REFVNVNYHLNGTINNFTGFSIDLFKAVMHNI--TMSYHFVPFHKTYNEMIDAVYNKTYD 540
           +EFVNV+   NGT  +F+GFSI +F  +M+NI    SY F  F+ +YN+MIDAVY K YD
Sbjct: 482 QEFVNVHRFENGT-EHFSGFSIAVFNEIMNNIKNMPSYKFARFNHSYNDMIDAVYMKEYD 541

Query: 541 GAVGDITILAERFRRVDFTVAYLKTDIVMVVTEKEEKWKKWWAFMEAFDFEVWLLIPTMH 600
           GAVGDITILA+RF++VDFTVAYLKTDIVMVV EK E+W+K WAFM+AF  +VW+LIPTMH
Sbjct: 542 GAVGDITILAKRFQQVDFTVAYLKTDIVMVVREKHERWRKLWAFMDAFKPQVWVLIPTMH 601

Query: 601 LFVSSIIWLIERQNNDELKGFGNMLWFSISIIFYIHREPVKNGLARLVLGPWLFAILVMT 660
           LF+SS+IWLIER+NN+ELKGFGNMLWFS+S+IFY+ REPVKN LARLVLGPWLFAI V+T
Sbjct: 602 LFISSLIWLIERENNEELKGFGNMLWFSVSLIFYMQREPVKNVLARLVLGPWLFAIFVVT 661

Query: 661 TSFSASLTSMMTISWSQPSVLDVETLKKMNATVGCNAESFIYNFLKNTLKFEPSKIVKMN 720
            SFSASLTSM+TISWSQPSV  VE LK+MNATVGCNAESFI N+LK+TL+FE S I +M 
Sbjct: 662 ASFSASLTSMLTISWSQPSVTTVEMLKEMNATVGCNAESFICNYLKDTLQFESSNIKRME 721

Query: 721 SIDDYPEAFKNDTIKAAFFISPHAEVFLAKNCRGYTKAVSSFKLGGIGFAFQKGSALAAM 780
           S+D+YP+AF++D+IKAAFFISPHA+VFL KNC+ YTK VSSFKLGGIGFAF KGS LAA 
Sbjct: 722 SLDNYPKAFEDDSIKAAFFISPHADVFLLKNCKRYTKGVSSFKLGGIGFAFPKGSDLAAK 781

BLAST of Lag0004272 vs. NCBI nr
Match: XP_038884568.1 (glutamate receptor 2.5-like [Benincasa hispida])

HSP 1 Score: 935.6 bits (2417), Expect = 2.7e-268
Identity = 537/850 (63.18%), Postives = 632/850 (74.35%), Query Frame = 0

Query: 1   MIRNIFC----FLGLLFLLLLLSSEAHTSKKEVNCQKTHRNESTRIGVVFDSGSQIGKQQ 60
           M+ N FC    FLGL+ L LLL SEAHTSKKE+ CQK +R  + R+GV+FDSGSQIGKQQ
Sbjct: 1   MLGNGFCCSSFFLGLVLLFLLLGSEAHTSKKELTCQKDNRT-NPRVGVIFDSGSQIGKQQ 60

Query: 61  MIAMKMALRRFRL-SSCV--KLELRLHD-SHPNFSSPTSSA------------------- 120
           M+AMKM LR F L SSCV  KLEL LHD SHPNF+SP SSA                   
Sbjct: 61  MVAMKMGLRDFHLASSCVKYKLELLLHDYSHPNFTSPPSSALDLITKGGVKAVIIGLSRK 120

Query: 121 -SQNPIFD------------SNFQQHHP---PNAMHCFNSD-----------PFSMAAKV 180
                IFD            S  Q  HP   P+ +   N++            F ++ KV
Sbjct: 121 QDSIVIFDHKILVGIPILSISTTQLLHPLKIPSLIQMANNNITHHIIASILTHFQLSPKV 180

Query: 181 TVFHEIN-----SFVSIRRLFDSFRSANIEIEHFLALSYSSSN--QAEILIEKELEKFMN 240
           T+F EI      S +S+ R FDSFR  NIEI   +ALS SSSN  QAEILIE EL+K MN
Sbjct: 181 TIFCEITNIHHPSNISVHRHFDSFRPVNIEINRLVALS-SSSNIIQAEILIENELKKLMN 240

Query: 241 SQRNNRVFIVTQLSLKLVRLLFTKAKNMNMVGNGYAWIVSSDVFDHVVSLD-SPSLLNKM 300
           +QRN  VFIVTQLSL+LV LLFTKAK MNM+GNGY WIVS +VFD + SLD S SLLNKM
Sbjct: 241 NQRNG-VFIVTQLSLELVDLLFTKAKKMNMIGNGYTWIVSHEVFDLISSLDSSSSLLNKM 300

Query: 301 EGVIGFRTYFDDTKKSFKSFETKLEKMYSLEYPQDKEPIKASIFTVRAYDASWAISKAIE 360
           EGVIGF+TYF+DTK S KSFETK +K+Y LEYPQ++EP KASIF ++AYDA+ AI+ A+E
Sbjct: 301 EGVIGFQTYFNDTKMSLKSFETKFKKIYKLEYPQEEEPTKASIFAIQAYDAAHAITSAME 360

Query: 361 KLGEK---FSSHQLMEKILESNFEGLSGMVRF---KNGMLISQAPTFQILKVVDGSLKDV 420
            +G +    SS +L EKILESNFEG+SGMVRF    NGMLI ++P F+I+KVVD + K+V
Sbjct: 361 NMGVENLSSSSEELKEKILESNFEGVSGMVRFSKNNNGMLIYESPNFKIIKVVDHAYKEV 420

Query: 421 AFWTPKLGFSESLVANNKELEFMEN---VAAVRHLSQTFSRELDGYGEIRRLKFAVPNQG 480
           AFWT K GF E  V  NK+     N      VR LS+    + +     R+L+FAVP QG
Sbjct: 421 AFWTGKFGFVEKSVEINKKEALRSNKMGKMGVRELSEMRVSKANSNNGERKLRFAVPGQG 480

Query: 481 ACREFVNVNYHLNGTINNFTGFSIDLFKAVMHNI--TMSYHFVPFHKTYNEMIDAVYNKT 540
           AC+EFVNV+Y+ NGT  NF+GFSID+ +AVM+NI    +Y   PF+ +Y++MI AVY+K 
Sbjct: 481 ACQEFVNVSYYSNGTAKNFSGFSIDVSRAVMNNIKNISAYDLFPFNHSYDDMIKAVYHKE 540

Query: 541 YDGAVGDITILAERFRRVDFTVAYLKTDIVMVVTEKEEKWKKWWAFMEAFDFEVWLLIPT 600
           YDGAVGDITILA+RF  VDFTVAYL TDIVMVVTEK+EKWK+ WAFMEAF+  VWLLIPT
Sbjct: 541 YDGAVGDITILAKRFEHVDFTVAYLNTDIVMVVTEKQEKWKRRWAFMEAFELPVWLLIPT 600

Query: 601 MHLFVSSIIWLIERQNNDELKGFGNMLWFSISIIFYIHREPVKNGLARLVLGPWLFAILV 660
           MH+FVS +IWLIE QNNDELKGFGNM+WFS+S IFY+HREPVKNGLARLV+GPWLFAILV
Sbjct: 601 MHIFVSFVIWLIEHQNNDELKGFGNMVWFSVSFIFYMHREPVKNGLARLVMGPWLFAILV 660

Query: 661 MTTSFSASLTSMMTISWSQPSVLDVETLKKM-NATVGCNAESFIYNFLKNTLKFEPSKIV 720
           +TTSFSASLTS+MT SWSQPSVLDVETLK+M +ATVGCN+ESFIYN+L +TL+FEPSKI 
Sbjct: 661 VTTSFSASLTSLMTNSWSQPSVLDVETLKQMPDATVGCNSESFIYNYLNSTLEFEPSKIK 720

Query: 721 KMNSIDDYPEAFKNDTIKAAFFISPHAEVFLAKNCRGYTKAVSSFKLGGIGFAFQKGSAL 775
           KMNSIDDYP+A KN +IKAAFFISPHA++FLAKNC+GYTKAVSSFKLGGIGFAF+KGS L
Sbjct: 721 KMNSIDDYPKALKNGSIKAAFFISPHADIFLAKNCKGYTKAVSSFKLGGIGFAFRKGSEL 780

BLAST of Lag0004272 vs. NCBI nr
Match: XP_022997325.1 (glutamate receptor 2.5-like [Cucurbita maxima])

HSP 1 Score: 913.3 bits (2359), Expect = 1.5e-261
Identity = 528/845 (62.49%), Postives = 620/845 (73.37%), Query Frame = 0

Query: 1   MIRNIF----CFLGLLFLLLLLSSEAHTSKKEVNCQKTHRNESTRIGVVFDSGSQIGKQQ 60
           ++RN F    CFLGLL LLLL S   H  + + N      N+ TRIG VFDSGSQIGKQQ
Sbjct: 2   ILRNGFCRPSCFLGLLLLLLLSSEGLHCQRNQPN------NKPTRIGAVFDSGSQIGKQQ 61

Query: 61  MIAMKMALRRFRL-SSCVKLELRLHDSHPNFSSPT---------------SSASQN---- 120
           M+AMKMALR F L SSC KL+L LHDSHPNF+S                 S  +Q+    
Sbjct: 62  MVAMKMALRHFHLSSSCHKLKLLLHDSHPNFTSSALDLITDGGVKAVVIGSVRTQDLVAF 121

Query: 121 ---------PIFDSNFQQHHP-------------PNAMHCFNS--DPFSMAAKVTVFHEI 180
                    PI   +  Q  P              + M C  S    F +  KVT F+EI
Sbjct: 122 SDHEIPVGVPILSISATQLQPKKIPSLIQMANNITHRMQCIASILTHFQLPPKVTFFYEI 181

Query: 181 N--------SFVSIRRLFDSFRSANIEIEHFLALSYSSSNQAEILIEKELEKFMNSQRNN 240
                    S +S  RLFDSFRS N+EI+H LALS SSSNQAEILIE EL++ +NSQRN 
Sbjct: 182 TNVDRPSEYSSISPDRLFDSFRSVNVEIDHRLALS-SSSNQAEILIENELKRVLNSQRNG 241

Query: 241 RVFIVTQLSLKLVRLLFTKAKNMNMVGNGYAWIVSSDVFDHVVSLD-SPSLLNKMEGVIG 300
            VF+VTQ+SL+L  LLFTKAK MNMVGNGY WIVS DV D + +LD S SLL KMEGVIG
Sbjct: 242 -VFVVTQISLELADLLFTKAKKMNMVGNGYTWIVSDDVLDLIPTLDSSSSLLYKMEGVIG 301

Query: 301 FRTYFDDTKKSFKSFETKLEKMYSLEYPQDKEPIKASIFTVRAYDASWAISKAIEKLGEK 360
           FRTYF+DTKKSFKSFET+ +KMY+LEYP+DK+PIKASIF VRAYDA  +I++A+E LG+ 
Sbjct: 302 FRTYFNDTKKSFKSFETRFKKMYNLEYPEDKDPIKASIFAVRAYDAVRSIARAMETLGDN 361

Query: 361 --FSSHQLMEKILESNFEGLSGMVRFKNGMLISQAPTFQILKVVDGSLKDVAFWTPKLGF 420
              SS QL+E ILESNFEGLSGMVRFKNGMLISQ+P F+I+KVVD S K VAFWTPK GF
Sbjct: 362 NLSSSDQLLETILESNFEGLSGMVRFKNGMLISQSPNFEIIKVVDQSYKPVAFWTPKSGF 421

Query: 421 SESLVANNKELEFMEN----VAAVRHLSQTFSRELDGYGEIRRLKFAVPNQGACREFVNV 480
           +ES   NNK+     N    +  VRHLS++F+RE DG GE + L FAVP QGAC EFVN+
Sbjct: 422 AESFEENNKKSALRSNNMGSMVGVRHLSESFTRE-DGDGE-KHLTFAVPGQGACHEFVNM 481

Query: 481 NYHLNGTINNFTGFSIDLFKAVMHNI--TMSYHFVPFHKTYNEMIDAVYNKTYDGAVGDI 540
           +   NGT  +F+GFSI +F  VM NI    S+ F  F+ +YN+MIDAVY K YDGAVGDI
Sbjct: 482 HTFENGT-EHFSGFSIAVFNEVMKNIKNMPSFKFARFNHSYNDMIDAVYRKEYDGAVGDI 541

Query: 541 TILAERFRRVDFTVAYLKTDIVMVVTEKEEKWKKWWAFMEAFDFEVWLLIPTMHLFVSSI 600
           TILA+RF++VDFTVAYLKTDIVMVV EK    +K WAFM+AF  EVW+LIPTMHLF+SS+
Sbjct: 542 TILAKRFQQVDFTVAYLKTDIVMVVREK--YGRKLWAFMDAFKPEVWVLIPTMHLFISSL 601

Query: 601 IWLIERQNNDELKGFGNMLWFSISIIFYIHREPVKNGLARLVLGPWLFAILVMTTSFSAS 660
           IWLIER+NN+ELKGFGNMLWFS+S+IFY  REPVKNGLARLVLGPWLFAI ++T SFSAS
Sbjct: 602 IWLIERKNNEELKGFGNMLWFSVSLIFYTQREPVKNGLARLVLGPWLFAIFMVTASFSAS 661

Query: 661 LTSMMTISWSQPSVLDVETLKKMNATVGCNAESFIYNFLKNTLKFEPSKIVKMNSIDDYP 720
           LTSM+TISWSQPSV  VE LK+MNATVGCNAESFI ++L  +LKFE ++I KMNSIDDYP
Sbjct: 662 LTSMLTISWSQPSVTTVEMLKEMNATVGCNAESFICDYLNGSLKFENARIKKMNSIDDYP 721

Query: 721 EAFKNDTIKAAFFISPHAEVFLAKNCRGYTKAVSSFKLGGIGFAFQKGSALAAMVSESIA 780
           +AF+N +I AAFFISPHA+VFL KNC+ YTK VSSFKLGGIGFAF KGS LAA VS+SIA
Sbjct: 722 KAFENGSITAAFFISPHADVFLVKNCKRYTKGVSSFKLGGIGFAFPKGSDLAAKVSKSIA 781

BLAST of Lag0004272 vs. NCBI nr
Match: XP_038884567.1 (glutamate receptor 2.5-like [Benincasa hispida])

HSP 1 Score: 912.5 bits (2357), Expect = 2.5e-261
Identity = 510/853 (59.79%), Postives = 622/853 (72.92%), Query Frame = 0

Query: 4   NIFCFLGLLFLLLLLSSEAHTS-KKEVNCQKTHRNESTRIGVVFDSGSQIGKQQMIAMKM 63
           ++F FLG    LLLL SEA+TS K E+NCQK   N++TRIGV FDSGSQIGKQQ++AMKM
Sbjct: 12  SLFFFLG----LLLLGSEAYTSIKLELNCQKNLPNKTTRIGVFFDSGSQIGKQQIVAMKM 71

Query: 64  ALRRFRLSSCVKLELRLHDSHPNFSSPTSSAS---------------------------- 123
           ALRRF  SSC KLEL LHDSH N+ + +S++S                            
Sbjct: 72  ALRRFHFSSCAKLELLLHDSHANYVNSSSASSVLDLITKGRVKAVVGSVRKQDLIVISDH 131

Query: 124 ----QNPIFDSNFQQHHP---------------PNAMHCFNS--DPFSMAAKVTVFHEIN 183
               + PI  ++ +Q  P                + +HC  S    F    KVT+F++  
Sbjct: 132 KIPVEIPIISTSAEQLQPLKIPSLIQMVDNNNITHPIHCIASILTYFECLPKVTIFYQFT 191

Query: 184 SFVSIRRLFDSFRSANIEIEHFLALSYSSSNQAEILIEKELEKFMNSQRNNRVFIVTQLS 243
           +  S   LFDSF  A IE+EH LALS +S+   EILIE+EL+K M+SQR NRVFIVTQLS
Sbjct: 192 N-TSAHCLFDSFLLAGIEVEHHLALSLASNQ--EILIEEELKKIMSSQR-NRVFIVTQLS 251

Query: 244 LKLVRLLFTKAKNMNMVGNGYAWIVSSDVFDHVVSLD-SPSLLNKMEGVIGFRTYFDDTK 303
           L+LV LL TKAK MNMVGNGY WIVS ++FD + SLD S SLLNKMEGVIGF+TYF+DTK
Sbjct: 252 LELVDLLLTKAKKMNMVGNGYTWIVSHEIFDLISSLDSSSSLLNKMEGVIGFQTYFNDTK 311

Query: 304 KSFKSFETKLEKMYSLEYPQDKEPIKASIFTVRAYDASWAISKAIEKLGE---KFSSHQL 363
           +SFK FETK +K+Y LEYPQ++EP KASI  +RAYDA+ AI++A+EKLG    + SS QL
Sbjct: 312 RSFKIFETKFKKIYKLEYPQEEEPTKASILAIRAYDAALAITRAMEKLGTENLRSSSEQL 371

Query: 364 MEKILESNFEGLSGMVRF--KNGMLISQAPTFQILKVVDGSLKDVAFWTPKLGFSESLVA 423
           M+KILESNFEG+ GMVRF  KNGMLISQ+P F+I+KVVD + K+V FWTP LGF E ++ 
Sbjct: 372 MKKILESNFEGVGGMVRFSKKNGMLISQSPKFKIIKVVDQTYKEVGFWTPILGFVERVIV 431

Query: 424 N--------NKELEFMENVAAVRHLSQ---TFSRELDGYGEIRRLKFAVPNQGACREFVN 483
                       +  + N   VR LS+   + S + D + E +RLKFAVP +GAC+EFV 
Sbjct: 432 EINKPTTNLKSNMRNLRNYVGVRDLSRLKASPSEKFDHHEE-KRLKFAVPEEGACKEFVK 491

Query: 484 VNYHLNGTINNFTGFSIDLFKAVMHNITM----SYHFVPFHKTYNEMIDAVYNKTYDGAV 543
           V++HL G  N  TG+SID+F+AVM+N+ M    SY  VPF  TY+EMI+AV NKTY GAV
Sbjct: 492 VSHHLYG--NYITGYSIDVFRAVMNNMNMSHPLSYDLVPFKGTYDEMIEAVSNKTYIGAV 551

Query: 544 GDITILAERFRRVDFTVAYLKTDIVMVVTEKEEKWKKWWAFMEAFDFEVWLLIPTMHLFV 603
           GDI ILA R++ VDFTV+YL+T+IVMVV EK EKWKK WAFMEAF F +WLLIPTMHLF+
Sbjct: 552 GDIGILARRYKYVDFTVSYLETEIVMVVKEKHEKWKKIWAFMEAFQFTMWLLIPTMHLFI 611

Query: 604 SSIIWLIERQNNDELKGFGNMLWFSISIIFYIHREPVKNGLARLVLGPWLFAILVMTTSF 663
           S +IW IERQNN+ELKG GNMLWFS+SIIFY+HREPVKNGLARLVLGPWLFAILV+T SF
Sbjct: 612 SFVIWFIERQNNEELKGLGNMLWFSVSIIFYMHREPVKNGLARLVLGPWLFAILVVTASF 671

Query: 664 SASLTSMMTISWSQPSVLDVETLKKMNATVGCNAESFIYNFLKNTLKFEPSKIVKMNSID 723
           +ASLTSMMTISWS+P V DV+TLK+M ATVGCN  SFI N+L  TL+F+P+KI K+NS++
Sbjct: 672 TASLTSMMTISWSRPLVPDVDTLKQMGATVGCNTNSFICNYLYETLQFDPTKIKKINSLN 731

Query: 724 DYPEAFKNDTIKAAFFISPHAEVFLAKNCRGYTKAVSSFKLGGIGFAFQKGSALAAMVSE 783
           +YP AF+N +IKAAFFISPHA+VFLAK C+GYT+ VSSFKL GIGFA  KGS L ++VS 
Sbjct: 732 EYPNAFENGSIKAAFFISPHAKVFLAKYCKGYTRGVSSFKLTGIGFALAKGSGLTSLVSA 791

Query: 784 SIANLAETNYTSEMEQKLLAS-HCPSTNKADGLGLGPGPFMGLFLICGCIALLVLLYMGL 785
           SI  L ET    + E  ++AS +C ST K +GLGLGPGPFMGLF+ICG IALLVL+YM  
Sbjct: 792 SIVELTETKEIPQFESNVIASFNCSSTGKGEGLGLGPGPFMGLFIICGSIALLVLIYMAQ 851

BLAST of Lag0004272 vs. ExPASy Swiss-Prot
Match: Q9LFN5 (Glutamate receptor 2.5 OS=Arabidopsis thaliana OX=3702 GN=GLR2.5 PE=2 SV=2)

HSP 1 Score: 280.8 bits (717), Expect = 4.8e-74
Identity = 211/690 (30.58%), Postives = 343/690 (49.71%), Query Frame = 0

Query: 141 LFDSFRSANIEIEHFLALSYSSSNQAEILIEKELEKFMNSQRNNRVFIVTQLSLKLVRLL 200
           L D+F+  N+ I +  A+S   S+     I+KEL K M      RVFIV  L   L   L
Sbjct: 189 LVDAFQEINVRIRYRSAISLHYSDDQ---IKKELYKLMTMP--TRVFIVHMLP-DLGSRL 248

Query: 201 FTKAKNMNMVGNGYAWIVSSDVFDHVVSLDSPSLLNKMEGVIGFRTYFDDTKKSFKSFET 260
           F+ AK ++M+  GY WIV++ + D +  +   SL+N M GV+G +TYF  +K+     E 
Sbjct: 249 FSIAKEIDMLSKGYVWIVTNGIADLMSIMGESSLVN-MHGVLGVKTYFAKSKE-LLHLEA 308

Query: 261 KLEKMYSLEYPQDKEPIKASIFTVRAYDASWAISKAIE---------------------- 320
           + +K +  E        + + F   AYDA+ A++ ++E                      
Sbjct: 309 RWQKRFGGE--------ELNNFACWAYDAATALAMSVEEIRHVNMSFNTTKEDTSRDDIG 368

Query: 321 ----KLGEKFSSHQLMEKILESNFEGLSGMVRFKNGMLISQAPTFQILKVVDGSLKDVAF 380
               +LG   S  +L++ +   +F+G++G  + KNG L  +A TF+I+ + +   + V F
Sbjct: 369 TDLDELGVALSGPKLLDALSTVSFKGVAGRFQLKNGKL--EATTFKIINIEESGERTVGF 428

Query: 381 WTPKLGFSESLVANNKELEFMENVAAVRHLSQTFSRELDGYGE-------------IRRL 440
           W  K+G  +SL            V  V H S+   R +   G+              ++L
Sbjct: 429 WKSKVGLVKSL-----------RVDKVSHSSRRL-RPIIWPGDTIFVPKGWEFPTNAKKL 488

Query: 441 KFAVPNQGACREFVNVNYHLNGTINNFTGFSIDLFKAVMHNI--TMSYHFVPF------- 500
           + AVP +     FV V    N  +   TGF ID+F  VM  +   +SY ++PF       
Sbjct: 489 RIAVPKKDGFNNFVEVTKDENTNVPTVTGFCIDVFNTVMSQMPYAVSYEYIPFDTPDGKP 548

Query: 501 HKTYNEMIDAVYNKTYDGAVGDITILAERFRRVDFTVAYLKTDIVMVVTEKEEKWKKWWA 560
             +Y+EM+  V+   +DGAVGD TILA R   VDF + Y +T IV +V  K+ K K  W 
Sbjct: 549 RGSYDEMVYNVFLGEFDGAVGDTTILANRSHYVDFALPYSETGIVFLVPVKDGKEKGEWV 608

Query: 561 FMEAFDFEVWLLIPTMHLFVSSIIWLIERQNNDELK------GFGNMLWFSISIIFYIHR 620
           F++    E+WL+     L++  ++W+ E Q ++E +         ++ +FS S +F+ HR
Sbjct: 609 FLKPLTKELWLVTAASFLYIGIMVWIFEYQADEEFREQMIIDKISSVFYFSFSTLFFAHR 668

Query: 621 EPVKNGLARLVLGPWLFAILVMTTSFSASLTSMMTISWSQPSVLDVETLKKMNATVGCNA 680
            P ++   R+++  W F +L++T S++A+LTSM+T+   +P+V  ++ L+K    +G   
Sbjct: 669 RPSESFFTRVLVVVWCFVLLILTQSYTATLTSMLTVQELRPTVRHMDDLRKSGVNIGYQT 728

Query: 681 ESFIYNFLKNTLKFEPSKIVKMNSIDDYPEAF----KNDTIKAAFFISPHAEVFLAKNCR 740
            SF +  LK  ++F+ S++   NS ++  E F     N  I AAF    + ++F+AK C 
Sbjct: 729 GSFTFERLKQ-MRFDESRLKTYNSPEEMRELFLHKSSNGGIDAAFDEVAYIKLFMAKYCS 788

Query: 741 GYTKAVSSFKLGGIGFAFQKGSALAAMVSESIANLAETNYTSEMEQK--LLASHC-PSTN 770
            Y+    +FK  G GFAF  GS L + +S  I N+ E +    +E K  L   HC  ST 
Sbjct: 789 EYSIIEPTFKADGFGFAFPLGSPLVSDISRQILNITEGDAMKAIENKWFLGEKHCLDSTT 847

BLAST of Lag0004272 vs. ExPASy Swiss-Prot
Match: Q9LFN8 (Glutamate receptor 2.6 OS=Arabidopsis thaliana OX=3702 GN=GLR2.6 PE=2 SV=2)

HSP 1 Score: 267.7 bits (683), Expect = 4.2e-70
Identity = 212/741 (28.61%), Postives = 352/741 (47.50%), Query Frame = 0

Query: 87  SSPTSSASQNPIFDSNFQQHHPPNAMHCFNS--DPFSMAAKVTVFHEINSFVS--IRRLF 146
           SSP   + ++P F      H   + +H  ++  + F     V ++ + N F    +  L 
Sbjct: 133 SSPVLDSLRSPYFIR--ATHDDSSQVHAISAIIESFRWREVVPIYAD-NEFGEGILPYLV 192

Query: 147 DSFRSANIEIEHFLALSYSSSNQAEILIEKELEKFMNSQRNNRVFIVTQLSLKLVRLLFT 206
           D+F+  N+ I +  A+S  S++    L++KEL K M      RVFIV  L   L   LF+
Sbjct: 193 DAFQEINVRIRYRSAISVHSTDD---LVKKELYKLMTMP--TRVFIVHMLP-DLGSRLFS 252

Query: 207 KAKNMNMVGNGYAWIVSSDVFDHVVSLDSPSLLNKMEGVIGFRTYFDDTKKSFKSFETKL 266
            AK + M+  GY WIV++ + D  +S+   S L  M GV+G +TYF  +K+     ET+ 
Sbjct: 253 IAKEIGMMTKGYVWIVTNGIADQ-MSVMGESSLENMHGVLGVKTYFSRSKE-LMYLETRW 312

Query: 267 EKMYSLEYPQDKEPIKASIFTVRAYDASWAISKAIEKLGEK------------------- 326
            K +  E        + + F    YD + A++ +IE++                      
Sbjct: 313 RKRFGGE--------ELNNFECWGYDTATALAMSIEEISSNVNMSFSQTKRNTSRDDTGT 372

Query: 327 --------FSSHQLMEKILESNFEGLSGMVRFKNGMLISQAPTFQILKVVDGSLKDVAFW 386
                    S  +L++ +   +F+G++G  + KNG L  +A TF+I+ + +   + V FW
Sbjct: 373 DLDDLSFALSGPKLLQALATVSFKGVAGRFQLKNGKL--EATTFKIVNIEESGERTVGFW 432

Query: 387 TPKLGFSESLVANNKELEFMENVAAVRHLSQTFSRELDGYG-----EIRRLKFAVPNQGA 446
             K+G  +SL  N   ++   +   +R +           G       ++L+ AVP +  
Sbjct: 433 KSKVGLVKSLRVNQTGIKISHSSHRLRPIIWPGDTIFVPKGWEFPTNAKKLRIAVPKKDG 492

Query: 447 CREFVNVNYHLNGTINNFTGFSIDLFKAVMHNI--TMSYHFVPFHK-------TYNEMID 506
              FV V    N      TGF ID+F   M  +   + Y ++PF         +Y+EM+ 
Sbjct: 493 FNNFVEVTKDANTNAPTITGFCIDVFDTAMRQMPYAVPYEYIPFETPDGKPRGSYDEMVY 552

Query: 507 AVYNKTYDGAVGDITILAERFRRVDFTVAYLKTDIVMVVTEKEEKWKKWWAFMEAFDFEV 566
            V+   +DGAVGD TILA R   VDF + Y +T IV+VV  K+E+ K  W F++    E+
Sbjct: 553 HVFLGEFDGAVGDTTILANRSTYVDFALPYSETGIVVVVPVKDEREKGKWVFLKPLTREL 612

Query: 567 WLLIPTMHLFVSSIIWLIERQNNDE------LKGFGNMLWFSISIIFYIHREPVKNGLAR 626
           W L     L++  ++W+ E Q + +      +    N+ +FS S +F+ H  P ++   R
Sbjct: 613 WFLTAASFLYIGIMVWIFEYQASGDFRKQSIINKISNVFYFSFSTLFFAHMRPSESIFTR 672

Query: 627 LVLGPWLFAILVMTTSFSASLTSMMTISWSQPSVLDVETLKKMNATVGCNAESFIYNFLK 686
           +++  W F +L++T S++A+LTSM+T+   +P+V  ++ L+     +G    SF +  LK
Sbjct: 673 VLVVVWCFVLLILTQSYTATLTSMLTVQELRPTVRHMDDLRNSGVNIGYQTGSFTFERLK 732

Query: 687 NTLKFEPSKIVKMNSIDDYPEAF----KNDTIKAAFFISPHAEVFLAKNCRGYTKAVSSF 746
             + ++ S++   ++  +  E F     N  I AAF    + ++F+AK C  YT    +F
Sbjct: 733 Q-MGYKESRLKTYDTPQEMHELFLKKSSNGGIDAAFDEVAYVKLFMAKYCSKYTIIEPTF 792

Query: 747 KLGGIGFAFQKGSALAAMVSESIANLAETNYTSEMEQKLL--ASHC-PSTNKADGLGLGP 770
           K  G GFAF  GS L   +S  I N+ E      +E K L    HC  ST     + L  
Sbjct: 793 KADGFGFAFPLGSPLVPDLSRQILNITEGETMKAIENKWLLGEKHCLDSTTSDSPIRLDH 851

BLAST of Lag0004272 vs. ExPASy Swiss-Prot
Match: O81078 (Glutamate receptor 2.9 OS=Arabidopsis thaliana OX=3702 GN=GLR2.9 PE=2 SV=1)

HSP 1 Score: 261.5 bits (667), Expect = 3.0e-68
Identity = 198/709 (27.93%), Postives = 344/709 (48.52%), Query Frame = 0

Query: 119 PFSMAAKVTVFHEINSFVSIRRLFDSFRSANIEI-----EHFLALSYSSSNQAEIL---- 178
           P+ + A +    ++ +  SI + F   R   I +     E F+   + +    E+     
Sbjct: 137 PYFVRATIDDSSQVRAIASIFKFFRWRRVVAIYVDNEFGEGFMPFLFDALQDVEVKRSVI 196

Query: 179 --------IEKELEKFMNSQRNNRVFIVTQLSLKLVRLLFTKAKNMNMVGNGYAWIVSSD 238
                   I+KEL K M  +R  RVF+V  +   L   +F  A+++ M+  GY W++++ 
Sbjct: 197 PPEAIDDEIQKELRKLM--ERQARVFVV-HMESSLALRVFQIARDIGMMEEGYVWLMTNG 256

Query: 239 VFDHVVSLDSPSLLNKMEGVIGFRTYFDDTKKSFKSFETKLEKMYSLEYPQDKEPIKASI 298
           +   +  +++   LN +EGV+G R++   +K+    F  + ++ +  E P  ++ +  ++
Sbjct: 257 MTHMMRHINNGRSLNTIEGVLGVRSHVPKSKE-LGDFRLRWKRTFEKENPSMRDDL--NV 316

Query: 299 FTVRAYDASWAISKAIEK-----------------------LGEKFSSHQLMEKILESNF 358
           F + AYD+  A++KA+EK                       +G       L +   E  F
Sbjct: 317 FALWAYDSITALAKAVEKANTKSLWYDNGSTLSKNRTDLGNVGVSLYGPSLQKAFSEVRF 376

Query: 359 EGLSGMVRFKNGMLISQAPTFQILKVVDGSLKDVAFWTPKLGFSESLVANNKELEFMENV 418
            GL+G  +  +G L  Q+P F+I+  V    + + FWTP+ G  ++  +N K L  +   
Sbjct: 377 NGLAGEFKLIDGQL--QSPKFEIINFVGNEERIIGFWTPRDGLMDATSSNKKTLGPVIWP 436

Query: 419 AAVRHLSQTFSRELDGYGEIRRLKFAVPNQGACREFVNVNYHLNGTINNFTGFSIDLFKA 478
              + + + +  E+ G    ++L+  VP +    +FV V  +        TG++I++F+A
Sbjct: 437 GKSKIVPKGW--EIPG----KKLRVGVPMKKGFFDFVKVTINPITNKKTPTGYAIEIFEA 496

Query: 479 VMHNI--TMSYHFVPFH--KTYNEMIDAVYNKTYDGAVGDITILAERFRRVDFTVAYLKT 538
            +  +   +   +V F     YN ++  VY+KT+D  VGDITI A R    DFT+ + ++
Sbjct: 497 ALKELPYLVIPEYVSFESPNNYNNLVYQVYDKTWDAVVGDITITANRSLYADFTLPFTES 556

Query: 539 DIVMVVTEKEEKWKKWWAFMEAFDFEVWLLIPTMHLFVSSIIWLIERQNNDELKG----- 598
            + M+V  ++ + K  W F+E +  E+W+      +F+  ++WL E + N + +G     
Sbjct: 557 GVSMMVPVRDNENKDTWVFLEPWSLELWVTTGCFFVFIGFVVWLFEHRVNTDFRGPPQYQ 616

Query: 599 FGNMLWFSISIIFYIHREPVKNGLARLVLGPWLFAILVMTTSFSASLTSMMTISWSQPSV 658
            G  LWFS S + + HRE V + LAR V+  W F +LV+T S++ASLTS +T+   QP+V
Sbjct: 617 IGTSLWFSFSTMVFAHRENVVSNLARFVVVVWCFVVLVLTQSYTASLTSFLTVQSLQPTV 676

Query: 659 LDVETLKKMNATVGCNAESFIYNFLKNTLKFEPSKIVKMNS---IDDYPEAFKNDTIKAA 718
            +V  L K    VG    +F+ + L   L F   ++   +S    DD     K+  I AA
Sbjct: 677 TNVNDLIKNRDCVGYQGGAFVKDILLG-LGFHEDQLKPFDSAKDADDLLSKGKSKGIAAA 736

Query: 719 FFISPHAEVFLAKNCRGYTKAVSSFKLGGIGFAFQKGSALAAMVSESIANLAETNYTSEM 772
           F    + +  L+++C  Y     +FK GG GFAF K S L    S +I NL + N T ++
Sbjct: 737 FDEVAYLKAILSQSCSKYVMVEPTFKTGGFGFAFPKNSPLTGEFSRAILNLTQNNVTQQI 796

BLAST of Lag0004272 vs. ExPASy Swiss-Prot
Match: Q8LGN0 (Glutamate receptor 2.7 OS=Arabidopsis thaliana OX=3702 GN=GLR2.7 PE=2 SV=3)

HSP 1 Score: 254.6 bits (649), Expect = 3.7e-66
Identity = 190/652 (29.14%), Postives = 311/652 (47.70%), Query Frame = 0

Query: 163 SNQAEILIEKELEKFMNSQRNNRVFIVTQLSLKLVRLLFTKAKNMNMVGNGYAWIVSSDV 222
           +N  +IL  KEL K M  Q   RVF+V  +   L    F KA+ + M+  GY W+++  V
Sbjct: 212 ANDDQIL--KELYKLMTMQ--TRVFVV-HMPPTLGFRFFQKAREIGMMEEGYVWLLTDGV 271

Query: 223 FDHVVSLDSPSLLNKMEGVIGFRTYFDDTKKSFKSFETKLEKMYSLEYPQDKEPIKASIF 282
            + + S +  S L  M+GV+G R++   +KK  K+F  + EKM    +P+     + +IF
Sbjct: 272 MNLLKSNERGSSLENMQGVLGVRSHIPKSKK-LKNFRLRWEKM----FPKKGNDEEMNIF 331

Query: 283 TVRAYDASWAISKAIEK-----------------------LGEKFSSHQLMEKILESNFE 342
            +RAYD+  A++ A+EK                       LG       L++ +    F 
Sbjct: 332 ALRAYDSITALAMAVEKTNIKSLRYDHPIASGNNKTNLGTLGVSRYGPSLLKALSNVRFN 391

Query: 343 GLSGMVRFKNGMLISQAPTFQILKVVDGSLKDVAFWTPKLGFSESLVANNKELEFMENVA 402
           GL+G     NG L  ++  F ++ ++    + +  W P  G   +   N   +   E + 
Sbjct: 392 GLAGEFELINGQL--ESSVFDVINIIGSEERIIGLWRPSNGIVNAKSKNTTSV-LGERLG 451

Query: 403 AVRHLSQTFSRELDGYGEI----RRLKFAVPNQGACREFVNVNYHLNGTINNFTGFSIDL 462
            V  +    S+++    +I    + L+  +P +    EFV+            TG+ I++
Sbjct: 452 PV--IWPGKSKDVPKGWQIPTNGKMLRVGIPVKKGFLEFVDAKIDPISNAMTPTGYCIEI 511

Query: 463 FKAVMHNITMS-----YHFVPFHKTYNEMIDAVYNKTYDGAVGDITILAERFRRVDFTVA 522
           F+AV+  +  S       F+   + Y+EM+  VY   YD  VGD+TI+A R   VDFT+ 
Sbjct: 512 FEAVLKKLPYSVIPKYIAFLSPDENYDEMVYQVYTGAYDAVVGDVTIVANRSLYVDFTLP 571

Query: 523 YLKTDIVMVVTEKEEKWKKWWAFMEAFDFEVWLLIPTMHLFVSSIIWLIERQNNDELKG- 582
           Y ++ + M+V  K+   K  W F+  +  ++W+      +F+  I+W++E + N + +G 
Sbjct: 572 YTESGVSMMVPLKDN--KNTWVFLRPWSLDLWVTTACFFVFIGFIVWILEHRVNTDFRGP 631

Query: 583 ----FGNMLWFSISIIFYIHREPVKNGLARLVLGPWLFAILVMTTSFSASLTSMMTISWS 642
                G   WF+ S + + HRE V + LAR V+  W F +LV+  S++A+LTS  T+   
Sbjct: 632 PHHQIGTSFWFAFSTMNFAHREKVVSNLARFVVLVWCFVVLVLIQSYTANLTSFFTVKLL 691

Query: 643 QPSVLDVETLKKMNATVGCNAESFIYNFLKNTLKFEPSKIVKMNSIDDYPEAFKNDTIKA 702
           QP+V + + L K N  +G    +F+   LK+   F+ S++    S  +  E F N TI A
Sbjct: 692 QPTVTNWKDLIKFNKNIGYQRGTFVRELLKSQ-GFDESQLKPFGSAVECDELFSNGTITA 751

Query: 703 AFFISPHAEVFLAKNCRGYTKAVSSFKLGGIGFAFQKGSALAAMVSESIANLAETNYTSE 762
           +F    + +V L++N   YT    SFK  G GF F K S L   VS +I N+ +      
Sbjct: 752 SFDEVAYIKVILSQNSSKYTMVEPSFKTAGFGFVFPKKSPLTDDVSRAILNVTQGEEMQH 811

Query: 763 MEQKLL--ASHCPSTNKA-DGLGLGPGPFMGLFLICGCIALLVLLYMGLQFM 775
           +E K     ++CP  N +     L    F GLFLI G  + L LL     F+
Sbjct: 812 IENKWFKKPNNCPDLNTSLSSNHLSLSSFWGLFLIAGIASFLALLIFVANFL 845

BLAST of Lag0004272 vs. ExPASy Swiss-Prot
Match: Q9C5V5 (Glutamate receptor 2.8 OS=Arabidopsis thaliana OX=3702 GN=GLR2.8 PE=2 SV=2)

HSP 1 Score: 240.0 bits (611), Expect = 9.4e-62
Identity = 192/678 (28.32%), Postives = 321/678 (47.35%), Query Frame = 0

Query: 141 LFDSFRSANIEIEHFLALSYSSSNQAEILIEKELEKFMNSQRNNRVFIVTQLSLKLVRLL 200
           LFD+ +   ++     ++  S +N  +IL  KEL K M   R  RVF+V  ++ +L   +
Sbjct: 185 LFDALQDVQVD----RSVIPSEANDDQIL--KELYKLMT--RQTRVFVV-HMASRLASRI 244

Query: 201 FTKAKNMNMVGNGYAWIVSSDVFDHVVSLDSPSLLNKMEGVIGFRTYFDDTKKSFKSFET 260
           F KA  + M+  GY W++++ +   +  +     LN ++GV+G R++   + K  + F  
Sbjct: 245 FEKATEIGMMEEGYVWLMTNGMTHMMRHIHHGRSLNTIDGVLGVRSHVPKS-KGLEDFRL 304

Query: 261 KLEKMYSLEYPQDKEPIKASIFTVRAYDASWAISKAIEK--------------------L 320
           + ++ +  E P  ++ +  SIF + AYD++ A++ A+EK                    L
Sbjct: 305 RWKRNFKKENPWLRDDL--SIFGLWAYDSTTALAMAVEKTNISSFPYNNASGSSNNMTDL 364

Query: 321 GEKFSSH---QLMEKILESNFEGLSGMVRFKNGMLISQAPTFQILKVVDGSLKDVAFWTP 380
           G    S     L+E + E  F GL+G     +  L  ++P F+I+  V    + V FWTP
Sbjct: 365 GTLHVSRYGPSLLEALSEIRFNGLAGRFNLIDRQL--ESPKFEIINFVGNEERIVGFWTP 424

Query: 381 KLGFSESLVANNKELEFMEN-----VAAVRHLSQTFSRELDGYGEIRRLKFAVPNQGACR 440
             G     V +NK   F        +   +        E+   G  +++K  VP +    
Sbjct: 425 SNGLVN--VNSNKTTSFTGERFGPLIWPGKSTIVPKGWEIPTNG--KKIKVGVPVKKGFF 484

Query: 441 EFVNVNYHLNGTINNFT---GFSIDLFKAVMHNITMS-----YHFVPFHKTYNEMIDAVY 500
            FV V   +   I N T   G++ID+F+A +  +  S     Y F      Y++++  V 
Sbjct: 485 NFVEV---ITDPITNITTPKGYAIDIFEAALKKLPYSVIPQYYRFESPDDDYDDLVYKVD 544

Query: 501 NKTYDGAVGDITILAERFRRVDFTVAYLKTDIVMVVTEKEEKWKKWWAFMEAFDFEVWLL 560
           N T D  VGD+TI A R    DFT+ Y ++ + M+V  ++ + K  W F++ +  ++W+ 
Sbjct: 545 NGTLDAVVGDVTITAYRSLYADFTLPYTESGVSMMVPVRDNENKNTWVFLKPWGLDLWVT 604

Query: 561 IPTMHLFVSSIIWLIERQNNDELKG-----FGNMLWFSISIIFYIHREPVKNGLARLVLG 620
                + +  ++WL E + N + +G      G   WFS S + + HRE V + LAR V+ 
Sbjct: 605 TACFFVLIGFVVWLFEHRVNTDFRGPPHHQIGTSFWFSFSTMVFAHREKVVSNLARFVVV 664

Query: 621 PWLFAILVMTTSFSASLTSMMTISWSQPSVLDVETLKKMNATVGCNAESFIYNFLKNTLK 680
            W F +LV+T S++A+LTS +T+   QP+ ++V+ L K    VG    +F+ +FL     
Sbjct: 665 VWCFVVLVLTQSYTANLTSFLTVQRFQPAAINVKDLIKNGDYVGYQHGAFVKDFLIKE-G 724

Query: 681 FEPSKIVKMNSIDDYPEAFKNDTIKAAFFISPHAEVFLAKNCRGYTKAVSSFKLGGIGFA 740
           F  SK+    S ++      N +I AAF    +    L++ C  Y     +FK  G GFA
Sbjct: 725 FNVSKLKPFGSSEECHALLSNGSISAAFDEVAYLRAILSQYCSKYAIVEPTFKTAGFGFA 784

Query: 741 FQKGSALAAMVSESIANLAETNYTSEMEQK--LLASHCPSTNKA-DGLGLGPGPFMGLFL 775
           F + S L   VS++I N+ + +    +E K  +  + CP    A     L    F GLFL
Sbjct: 785 FPRNSPLTGDVSKAILNVTQGDEMQHIENKWFMKQNDCPDPKTALSSNRLSLRSFWGLFL 840

BLAST of Lag0004272 vs. ExPASy TrEMBL
Match: A0A6J1HC77 (glutamate receptor 2.5-like OS=Cucurbita moschata OX=3662 GN=LOC111462756 PE=3 SV=1)

HSP 1 Score: 948.0 bits (2449), Expect = 2.6e-272
Identity = 536/845 (63.43%), Postives = 629/845 (74.44%), Query Frame = 0

Query: 1   MIRNIF----CFLGLLFLLLLLSSEAHTSKKEVNCQKTHRNESTRIGVVFDSGSQIGKQQ 60
           ++RN F    CFLGLL LLLL S   H  + + N      N++TRIG VFDSGSQIGKQQ
Sbjct: 2   ILRNGFCRPSCFLGLLLLLLLSSEGLHCQRNQPN------NKTTRIGAVFDSGSQIGKQQ 61

Query: 61  MIAMKMALRRFRL-SSCVKLELRLHDSHPNFSSPTSSASQN------------------- 120
           M+AMKM LR F L SSC KLEL LHDSHPNF+S       N                   
Sbjct: 62  MVAMKMGLRHFHLSSSCHKLELLLHDSHPNFTSSALDLITNGGVKAVVIGSVRAQDLIAI 121

Query: 121 ---------PIFDSNFQQHHP-------------PNAMHCFNS--DPFSMAAKVTVFHEI 180
                    PI   +  Q HP              + M C  S    F +  KVTVF+EI
Sbjct: 122 SDHEIPVGVPILSISATQLHPKKIPSLIQMANNITHRMQCIVSILTHFQLPPKVTVFYEI 181

Query: 181 N--------SFVSIRRLFDSFRSANIEIEHFLALSYSSSNQAEILIEKELEKFMNSQRNN 240
                    S +S+ RLFDSFR  N+EI+H LALS SSSNQAEILIE EL+  M SQRN 
Sbjct: 182 TNIDHPSEYSSISVDRLFDSFRLVNVEIDHRLALS-SSSNQAEILIENELKSAMKSQRNG 241

Query: 241 RVFIVTQLSLKLVRLLFTKAKNMNMVGNGYAWIVSSDVFDHVVSLD-SPSLLNKMEGVIG 300
            VF+VTQLSL+L  LLFTKAK +NMVGNGY WIVS DV D + SLD S SLL KMEGVIG
Sbjct: 242 -VFVVTQLSLELADLLFTKAKKLNMVGNGYTWIVSDDVLDLIPSLDSSSSLLYKMEGVIG 301

Query: 301 FRTYFDDTKKSFKSFETKLEKMYSLEYPQDKEPIKASIFTVRAYDASWAISKAIEKLGEK 360
           FRTYF+DTKKSFKSFETK +KMY+LEYP+DKEPIKASIF VRAYD   +I++A++ LG+ 
Sbjct: 302 FRTYFNDTKKSFKSFETKFKKMYNLEYPEDKEPIKASIFAVRAYDVVRSIARAMKTLGDN 361

Query: 361 --FSSHQLMEKILESNFEGLSGMVRFKNGMLISQAPTFQILKVVDGSLKDVAFWTPKLGF 420
              SS QL+E ILESNFEGLSGMVRFKNGMLIS++P F+I+KVVD S K VAFWTPK GF
Sbjct: 362 NLSSSDQLLETILESNFEGLSGMVRFKNGMLISRSPNFEIIKVVDQSYKPVAFWTPKSGF 421

Query: 421 SESLVANNKELEF----MENVAAVRHLSQTFSRELDGYGEIRRLKFAVPNQGACREFVNV 480
           +ES V NNK+       M N+  VRHLS++F+RE DG GE ++L FAVP QGAC+EFVNV
Sbjct: 422 AESFVENNKKSALRSNNMGNMVGVRHLSESFTRE-DGDGE-KQLTFAVPGQGACQEFVNV 481

Query: 481 NYHLNGTINNFTGFSIDLFKAVMHNI--TMSYHFVPFHKTYNEMIDAVYNKTYDGAVGDI 540
           +   NGT  +F+GFSI +F  +M+NI    SY F  F+ +YN+MIDAVY K YDGAVGDI
Sbjct: 482 HTFENGT-EHFSGFSIAVFNEIMNNIKNMPSYKFARFNHSYNDMIDAVYVKEYDGAVGDI 541

Query: 541 TILAERFRRVDFTVAYLKTDIVMVVTEKEEKWKKWWAFMEAFDFEVWLLIPTMHLFVSSI 600
           TILA+RF++VDFTVAYLKTDIVMVV EK E+W+K WAFM+AF  +VW+LIPTMHLF+SS+
Sbjct: 542 TILAKRFQQVDFTVAYLKTDIVMVVREKHERWRKLWAFMDAFKPQVWVLIPTMHLFISSL 601

Query: 601 IWLIERQNNDELKGFGNMLWFSISIIFYIHREPVKNGLARLVLGPWLFAILVMTTSFSAS 660
           IWLIER+NN+ELKGFGNMLWFS+S+IFY+ REPVKNGLARLVLGPWLFAI V+T SFSAS
Sbjct: 602 IWLIERENNEELKGFGNMLWFSVSLIFYMQREPVKNGLARLVLGPWLFAIFVVTASFSAS 661

Query: 661 LTSMMTISWSQPSVLDVETLKKMNATVGCNAESFIYNFLKNTLKFEPSKIVKMNSIDDYP 720
           LTSM+TISWSQPSV  VE LK+MNATVGCNAESFI N+LK+TL+FE S I +M S+D+YP
Sbjct: 662 LTSMLTISWSQPSVTTVEMLKEMNATVGCNAESFICNYLKDTLQFESSNIKRMESLDNYP 721

Query: 721 EAFKNDTIKAAFFISPHAEVFLAKNCRGYTKAVSSFKLGGIGFAFQKGSALAAMVSESIA 780
           +AF+++TIKAAFFISPHA+VFLAKNCRGYTK VSSFKLGGIGFAF KGS  AA VS+SIA
Sbjct: 722 KAFEDNTIKAAFFISPHADVFLAKNCRGYTKGVSSFKLGGIGFAFPKGSDFAAKVSKSIA 781

BLAST of Lag0004272 vs. ExPASy TrEMBL
Match: A0A6J1K9A2 (glutamate receptor 2.5-like OS=Cucurbita maxima OX=3661 GN=LOC111492270 PE=3 SV=1)

HSP 1 Score: 913.3 bits (2359), Expect = 7.0e-262
Identity = 528/845 (62.49%), Postives = 620/845 (73.37%), Query Frame = 0

Query: 1   MIRNIF----CFLGLLFLLLLLSSEAHTSKKEVNCQKTHRNESTRIGVVFDSGSQIGKQQ 60
           ++RN F    CFLGLL LLLL S   H  + + N      N+ TRIG VFDSGSQIGKQQ
Sbjct: 2   ILRNGFCRPSCFLGLLLLLLLSSEGLHCQRNQPN------NKPTRIGAVFDSGSQIGKQQ 61

Query: 61  MIAMKMALRRFRL-SSCVKLELRLHDSHPNFSSPT---------------SSASQN---- 120
           M+AMKMALR F L SSC KL+L LHDSHPNF+S                 S  +Q+    
Sbjct: 62  MVAMKMALRHFHLSSSCHKLKLLLHDSHPNFTSSALDLITDGGVKAVVIGSVRTQDLVAF 121

Query: 121 ---------PIFDSNFQQHHP-------------PNAMHCFNS--DPFSMAAKVTVFHEI 180
                    PI   +  Q  P              + M C  S    F +  KVT F+EI
Sbjct: 122 SDHEIPVGVPILSISATQLQPKKIPSLIQMANNITHRMQCIASILTHFQLPPKVTFFYEI 181

Query: 181 N--------SFVSIRRLFDSFRSANIEIEHFLALSYSSSNQAEILIEKELEKFMNSQRNN 240
                    S +S  RLFDSFRS N+EI+H LALS SSSNQAEILIE EL++ +NSQRN 
Sbjct: 182 TNVDRPSEYSSISPDRLFDSFRSVNVEIDHRLALS-SSSNQAEILIENELKRVLNSQRNG 241

Query: 241 RVFIVTQLSLKLVRLLFTKAKNMNMVGNGYAWIVSSDVFDHVVSLD-SPSLLNKMEGVIG 300
            VF+VTQ+SL+L  LLFTKAK MNMVGNGY WIVS DV D + +LD S SLL KMEGVIG
Sbjct: 242 -VFVVTQISLELADLLFTKAKKMNMVGNGYTWIVSDDVLDLIPTLDSSSSLLYKMEGVIG 301

Query: 301 FRTYFDDTKKSFKSFETKLEKMYSLEYPQDKEPIKASIFTVRAYDASWAISKAIEKLGEK 360
           FRTYF+DTKKSFKSFET+ +KMY+LEYP+DK+PIKASIF VRAYDA  +I++A+E LG+ 
Sbjct: 302 FRTYFNDTKKSFKSFETRFKKMYNLEYPEDKDPIKASIFAVRAYDAVRSIARAMETLGDN 361

Query: 361 --FSSHQLMEKILESNFEGLSGMVRFKNGMLISQAPTFQILKVVDGSLKDVAFWTPKLGF 420
              SS QL+E ILESNFEGLSGMVRFKNGMLISQ+P F+I+KVVD S K VAFWTPK GF
Sbjct: 362 NLSSSDQLLETILESNFEGLSGMVRFKNGMLISQSPNFEIIKVVDQSYKPVAFWTPKSGF 421

Query: 421 SESLVANNKELEFMEN----VAAVRHLSQTFSRELDGYGEIRRLKFAVPNQGACREFVNV 480
           +ES   NNK+     N    +  VRHLS++F+RE DG GE + L FAVP QGAC EFVN+
Sbjct: 422 AESFEENNKKSALRSNNMGSMVGVRHLSESFTRE-DGDGE-KHLTFAVPGQGACHEFVNM 481

Query: 481 NYHLNGTINNFTGFSIDLFKAVMHNI--TMSYHFVPFHKTYNEMIDAVYNKTYDGAVGDI 540
           +   NGT  +F+GFSI +F  VM NI    S+ F  F+ +YN+MIDAVY K YDGAVGDI
Sbjct: 482 HTFENGT-EHFSGFSIAVFNEVMKNIKNMPSFKFARFNHSYNDMIDAVYRKEYDGAVGDI 541

Query: 541 TILAERFRRVDFTVAYLKTDIVMVVTEKEEKWKKWWAFMEAFDFEVWLLIPTMHLFVSSI 600
           TILA+RF++VDFTVAYLKTDIVMVV EK    +K WAFM+AF  EVW+LIPTMHLF+SS+
Sbjct: 542 TILAKRFQQVDFTVAYLKTDIVMVVREK--YGRKLWAFMDAFKPEVWVLIPTMHLFISSL 601

Query: 601 IWLIERQNNDELKGFGNMLWFSISIIFYIHREPVKNGLARLVLGPWLFAILVMTTSFSAS 660
           IWLIER+NN+ELKGFGNMLWFS+S+IFY  REPVKNGLARLVLGPWLFAI ++T SFSAS
Sbjct: 602 IWLIERKNNEELKGFGNMLWFSVSLIFYTQREPVKNGLARLVLGPWLFAIFMVTASFSAS 661

Query: 661 LTSMMTISWSQPSVLDVETLKKMNATVGCNAESFIYNFLKNTLKFEPSKIVKMNSIDDYP 720
           LTSM+TISWSQPSV  VE LK+MNATVGCNAESFI ++L  +LKFE ++I KMNSIDDYP
Sbjct: 662 LTSMLTISWSQPSVTTVEMLKEMNATVGCNAESFICDYLNGSLKFENARIKKMNSIDDYP 721

Query: 721 EAFKNDTIKAAFFISPHAEVFLAKNCRGYTKAVSSFKLGGIGFAFQKGSALAAMVSESIA 780
           +AF+N +I AAFFISPHA+VFL KNC+ YTK VSSFKLGGIGFAF KGS LAA VS+SIA
Sbjct: 722 KAFENGSITAAFFISPHADVFLVKNCKRYTKGVSSFKLGGIGFAFPKGSDLAAKVSKSIA 781

BLAST of Lag0004272 vs. ExPASy TrEMBL
Match: A0A6J1K7S1 (glutamate receptor 2.5-like OS=Cucurbita maxima OX=3661 GN=LOC111492435 PE=3 SV=1)

HSP 1 Score: 877.1 bits (2265), Expect = 5.6e-251
Identity = 507/855 (59.30%), Postives = 605/855 (70.76%), Query Frame = 0

Query: 1   MIRNIF----CFLGLLFLLLLLSSEAHTSKKEVNCQKT-HRNESTRIGVVFDSGSQIGKQ 60
           ++RN F    CFLGLL LLLL SSEAHT+KK +  QK    N++TRIGVVFDSGSQIGKQ
Sbjct: 2   VLRNAFRRPPCFLGLLLLLLLPSSEAHTTKKGLKGQKNPTNNKTTRIGVVFDSGSQIGKQ 61

Query: 61  QMIAMKMALRRFRLSSCV-KLELRLHDSHPNFSSPTSSA--------------------- 120
           Q +AMKM LR F LSS   KLEL LHDSHPNF+SPTSSA                     
Sbjct: 62  QKVAMKMGLRLFHLSSSSHKLELLLHDSHPNFTSPTSSAVDLMITKGGVKAIVGAVKRQD 121

Query: 121 ----------SQNPIFDSNFQQHHP----------PNAMH---CFNS--DPFSMAAKVTV 180
                      + PI  ++ ++  P           N  H   C  S    F    KV++
Sbjct: 122 LIVISDKEILGRIPIVSTSAEKLPPLKIPFLIQMANNITHHIKCIASILKYFQKPPKVSI 181

Query: 181 FHEINSFVSIRRLFDSFRSANIEIEHFLALSYSSSNQAEILIEKELEKFMNSQRNNRVFI 240
           F++  +F S+ RLFDSF+ ANIE+E+  ALS SSSNQ EI IE EL++ M SQRN  VF+
Sbjct: 182 FYD-PTFNSVHRLFDSFQLANIEVENHFALS-SSSNQDEISIENELKRVMKSQRNG-VFV 241

Query: 241 VTQLSLKLVRLLFTKAKNMNMVGNGYAWIVSSDVFDHVVSL---DSPSLLNKMEGVIGFR 300
           VTQLSL+LV LLF KAK MNMVGNGY WIVS+DVFD + S    D   LLNKMEGVIGFR
Sbjct: 242 VTQLSLELVNLLFIKAKKMNMVGNGYTWIVSNDVFDLIDSSLINDHSPLLNKMEGVIGFR 301

Query: 301 TYFDDTKKSFKSFETKLEKMYSLEYPQDKEPIKASIFTVRAYDASWAISKAIEKLGEK-- 360
           TYFDDTK  FK FETK +KMY+LEYP+D+EPIKAS F VRAYDA   I++A+E LG+   
Sbjct: 302 TYFDDTKMPFKRFETKFKKMYTLEYPEDEEPIKASSFAVRAYDAVRCIARAMETLGDNNL 361

Query: 361 FSSHQLMEKILESNFEGLSGMVRFKNGMLISQAPTFQILKVVDGSLKDVAFWTPKLGFSE 420
            SS Q+++ ILESNFEG+SGMVRFK+GMLI Q+P F+I+KVVD   KDVAFW+PKLGFSE
Sbjct: 362 RSSDQVLKTILESNFEGVSGMVRFKDGMLIPQSPNFKIIKVVDQRYKDVAFWSPKLGFSE 421

Query: 421 SLVANNKELEFMENVAAVRHLSQTFSRELDG--------------YGEIRRLKFAVPNQG 480
           S       LE  +N AA++ +     R +DG               GE  +LKFAVP +G
Sbjct: 422 SF------LEVEDNKAAIK-VKPNMGRHVDGGDLSRKAITLWSQSSGEEGKLKFAVPRKG 481

Query: 481 ACREFVNVNYHLNGTINNFTGFSIDLFKAVMHNITM----SYHFVPFHKTYNEMIDAVYN 540
           AC+E V V+ HLNG  N FTG SID+FKA M NI M    SY   PF  TY EM+ AV+N
Sbjct: 482 ACQELVKVSQHLNG--NYFTGLSIDVFKAAMTNINMPYSSSYDLYPFDGTYEEMMKAVHN 541

Query: 541 KTYDGAVGDITILAERFRRVDFTVAYLKTDIVMVVTEKEEKWKKWWAFMEAFDFEVWLLI 600
           KTYDGAVGDI+I+A+RF  VDF+VAYL+ DIVMVV E+++KWK  W F EAF+  VWLLI
Sbjct: 542 KTYDGAVGDISIMAQRFESVDFSVAYLEADIVMVVKEEQDKWKSLWVFFEAFEVTVWLLI 601

Query: 601 PTMHLFVSSIIWLIERQNNDELKGFGNMLWFSISIIFYIHREPVKNGLARLVLGPWLFAI 660
           PT+HLF+SS+IW+IER+NN+ELKG G+MLWFS+S+I Y  REPVKNGL+RLVLGPWLF I
Sbjct: 602 PTIHLFISSVIWVIERRNNEELKGLGSMLWFSVSLISYTQREPVKNGLSRLVLGPWLFGI 661

Query: 661 LVMTTSFSASLTSMMTISWSQPSVLDVETLKKMNATVGCNAESFIYNFLKNTLKFEPSKI 720
           LV+T SFSASLTSMMT+SWSQP + DV+TLKKM+A+VGCN ESFI N+L  +LKFE +KI
Sbjct: 662 LVVTESFSASLTSMMTVSWSQPLIPDVKTLKKMDASVGCNTESFICNYLNGSLKFENAKI 721

Query: 721 VKMNSIDDYPEAFKNDTIKAAFFISPHAEVFLAKNCRGYTKAVSSFKLGGIGFAFQKGSA 780
            KMN+IDDYP+A  N +IKAAF I PHA+VFLAK C GYTK   S KLGGIGFAF+KGSA
Sbjct: 722 KKMNTIDDYPKALDNGSIKAAFLIRPHADVFLAKYCIGYTKTNFSIKLGGIGFAFRKGSA 781

BLAST of Lag0004272 vs. ExPASy TrEMBL
Match: A0A1S3BAP7 (glutamate receptor 2.5-like OS=Cucumis melo OX=3656 GN=LOC103487885 PE=3 SV=1)

HSP 1 Score: 851.7 bits (2199), Expect = 2.5e-243
Identity = 494/847 (58.32%), Postives = 604/847 (71.31%), Query Frame = 0

Query: 8   FLGLLFLLLLLSSEAHTSKKEVN-CQK--THRNESTR-IGVVFDSGSQIGKQQMIAMKMA 67
           FLGL+FL+L L SEAHT++KE+N CQK     N + R IG+V DSGSQIGKQQ++A+KM 
Sbjct: 12  FLGLVFLVLFLGSEAHTNQKELNYCQKNLAINNVTARIIGLVSDSGSQIGKQQIVAIKMG 71

Query: 68  LRRFRL--SSCVKLELRLHDSHPNFSSPTSSA---------------------------- 127
           LR F L  SSCVKLEL LHDSHPN +S  SSA                            
Sbjct: 72  LRDFSLASSSCVKLELLLHDSHPNLTSSPSSALELITKGGVKAVIIIGSSRKQDLIVNIS 131

Query: 128 ---------SQNP--------------IFDSNFQQHHPPNAMHCFNS--DPFSMAAKVTV 187
                    S NP              I ++N   HH    + C +S         KV+V
Sbjct: 132 DHEIPIISISNNPQLLVNPLKIPSLIQISNTNPNHHH----IQCISSILTHLHFHQKVSV 191

Query: 188 FHEINSF--VSIRRLFDSFRSANIEIEHFLALSYSSS-NQAEILIEKELEKFMNSQRNNR 247
           F+E+NS   VS  RLF SF+S NIEI+H LAL  SS+  QAEILIEKEL++ +NSQ +NR
Sbjct: 192 FYELNSSTDVSPHRLFHSFQSVNIEIDHLLALPPSSNIIQAEILIEKELKRLLNSQ-SNR 251

Query: 248 VFIVTQLSLKLVRLLFTKAKNMNMVGNGYAWIVSSDVFDHVVSLD---SPSLLNKMEGVI 307
           VFI+TQLSL+LV LL TKAK MNMVGNGY WI+S +VFD + SLD   S SLLNKMEGVI
Sbjct: 252 VFIITQLSLQLVDLLLTKAKKMNMVGNGYTWIISHEVFDLISSLDSSSSSSLLNKMEGVI 311

Query: 308 GFRTYFDDTKKSFKSFETKLEKMYSLEYPQDKEPIKASIFTVRAYDASWAISKAIEKLGE 367
           G +TYF+DTKKSFKSFETK +K Y LEYPQ++EP K SIF ++AYDA+ AI++A+E L  
Sbjct: 312 GLQTYFNDTKKSFKSFETKFKKFYKLEYPQEEEPTKVSIFAIQAYDAAHAITRAMENL-- 371

Query: 368 KFSSHQLMEKILESNFEGLSG-MVRF------KNGMLIS--QAPTFQILKVVDGSLKDVA 427
           + S H+LMEKIL+ NF+G+SG MVRF       N MLIS   +P+F+I+KVVD + K+VA
Sbjct: 372 RSSDHELMEKILKINFKGVSGIMVRFSKNHNNNNEMLISSQSSPSFKIIKVVDHTYKEVA 431

Query: 428 FWTPKLGFSESLVANNKELEFMENVAAVRHLSQTFSRELDGYGEIRRLKFAVPNQGACRE 487
           FWTPKLGF E  V  +K   +  +++ +R        E    GE R+L F VP QGAC+E
Sbjct: 432 FWTPKLGFVEKYVRVSKNY-YKRSLSEMR----VSKSEKSSNGE-RKLTFVVPRQGACQE 491

Query: 488 FVNVNYHLNGTINNFTGFSIDLFKAVMHNI--TMSYHFVPF-HKTYNEMIDAVYNKTYDG 547
           FVNV+Y+ NGT+ N TGFS+D+F+AVM+NI    SY   PF H++Y+ MIDAV NK +DG
Sbjct: 492 FVNVSYYSNGTVQNITGFSVDVFRAVMNNIKDISSYELHPFHHQSYDRMIDAVSNKKFDG 551

Query: 548 AVGDITILAERFRRVDFTVAYLKTDIVMVVTEKEEKWKKWWAFMEAFDFEVWLLIPTMHL 607
           AVGDITILA RF+ VDFTVAYLKTDIVMVVTEK+EKWK+ WAFM+AF + VW+++PTMH+
Sbjct: 552 AVGDITILARRFKSVDFTVAYLKTDIVMVVTEKQEKWKRLWAFMDAFQYPVWIILPTMHI 611

Query: 608 FVSSIIWLIERQNNDELKGFGNMLWFSISIIFYIHREPVKNGLARLVLGPWLFAILVMTT 667
           F+S +IWL E  NN +L+ FGNMLWFS+S+IF++HRE V++GL RL+LGPWLF +LV+TT
Sbjct: 612 FISFVIWLTEFPNNQDLRSFGNMLWFSVSVIFHVHREQVRSGLTRLMLGPWLFTMLVVTT 671

Query: 668 SFSASLTSMMTISWSQPSVLDVETLKKM--NATVGCNAESFIYNFLKNTLKFEPSKIVKM 727
           SFSASLTS+MT SWSQPSV DVETLK+   NATVGCNAESFIY++L  TL+F+ S++  M
Sbjct: 672 SFSASLTSLMTNSWSQPSVPDVETLKQTMPNATVGCNAESFIYDYLTTTLEFDKSRVKTM 731

Query: 728 NSIDDYPEAFKNDTIKAAFFISPHAEVFLAKNCRGYTKAVSSFKLGGIGFAFQKGSALAA 775
            SIDDYPEA KN +I AAFFISPHA +FLAKN +GYTKAVSSFKLGG+GFAF KGS LA 
Sbjct: 732 KSIDDYPEALKNGSISAAFFISPHANIFLAKNRKGYTKAVSSFKLGGMGFAFPKGSELAM 791

BLAST of Lag0004272 vs. ExPASy TrEMBL
Match: A0A6J1K353 (glutamate receptor 2.5-like OS=Cucurbita maxima OX=3661 GN=LOC111491966 PE=3 SV=1)

HSP 1 Score: 836.6 bits (2160), Expect = 8.4e-239
Identity = 489/852 (57.39%), Postives = 597/852 (70.07%), Query Frame = 0

Query: 1   MIRNIFC----FLGLLFLLLLLSSEAHTSKKEVNCQKTHR--NESTRIGVVFDSGSQIGK 60
           ++R  FC     LGLL L   L SEA TSK  +NCQ   +  +++TRIGVVFDSGSQ+GK
Sbjct: 4   VLRICFCRLSFILGLLLLSSSLGSEALTSKSILNCQTNPKPNDKTTRIGVVFDSGSQLGK 63

Query: 61  QQMIAMKMALRRFRL-SSCVKLELRLHDSHPNFSSPTSSA-----------------SQN 120
           QQ++AMKM L  F L SSC+KLEL LHDSH N +SP SSA                  Q+
Sbjct: 64  QQLVAMKMGLSHFHLSSSCLKLELLLHDSHQNLTSPPSSALDLITKGGVKAVVGSVRMQD 123

Query: 121 PIFDSN----------------FQQHHPPNAMHCFNSDPFS-------------MAAKVT 180
            I  SN                 +Q   P+ +   N    +                KVT
Sbjct: 124 LIVISNNNFPVPIPIVSTSAEQLEQLKIPSLIQMANHITITHRIQCIASILTHFQWRKVT 183

Query: 181 VFHEIN--------SFVSIRRLFDSFRSANIEIEHFLALSYSSSNQAEILIEKELEKFMN 240
           +F++I+        S VS  RLFDS R A++E+EH LALS SSSNQ EILIE+EL+K MN
Sbjct: 184 IFYDISNTDHSPIYSAVSANRLFDSLRLADVEVEHRLALS-SSSNQ-EILIEQELKKLMN 243

Query: 241 SQRNNRVFIVTQLSLKLVRLLFTKAKNMNMVGNGYAWIVSSDVFDHVVSLDSPSLLNKME 300
           SQR NRVF+VTQL ++L  L+  +AK +NMVGNGY WIVS+DVFD + SLDS S   KME
Sbjct: 244 SQR-NRVFVVTQLEVELAVLVLEQAKKLNMVGNGYVWIVSNDVFDAIDSLDS-SFWYKME 303

Query: 301 GVIGFRTYFDDTKKSFKSFETKLEKMYSLEYPQDKEPIKASIFTVRAYDASWAISKAIEK 360
           GVIGFRTYFDDTK SFKSFETK +KMY LEYPQ++EP +ASI  VRAYDA+ AI++A   
Sbjct: 304 GVIGFRTYFDDTKNSFKSFETKFKKMYRLEYPQEEEPTRASISVVRAYDAARAITRA--- 363

Query: 361 LGEKFSSHQLMEKILESNFEGLSGMVRFKNGMLISQAPTFQILKVVDGSLKDVAFWTPKL 420
           +GE  S  +++EKI ESNFEGLSGMVRFKNGMLISQ+P F+I KVV  S K+V FWTPKL
Sbjct: 364 MGENLSWSEVLEKIGESNFEGLSGMVRFKNGMLISQSPNFKISKVVGQSFKEVGFWTPKL 423

Query: 421 GFSESLVANNKELEFME------NVAAVRHLSQ--TFSRELDGYGEIRRLKFAVPNQGAC 480
           GF E  V  NK    ++         AV  L +  T S   DG    +RL+FAVP +GAC
Sbjct: 424 GFVERFVEVNKTTTKLKPKFGNLGNVAVGDLPRLTTSSENCDGE---KRLRFAVPEEGAC 483

Query: 481 REFVNVNYHLNGTINNFTGFSIDLFKAVMHNIT----MSYHFVPFHKTYNEMIDAVYNKT 540
           +E V V+ HL G     TGFSI++F+AVM NI     +SY  +PF   Y +M++AV NKT
Sbjct: 484 QEIVKVSKHLEGYY--VTGFSINVFRAVMSNINISHPLSYDLLPFKGKYEDMLEAVRNKT 543

Query: 541 YDGAVGDITILAERFRRVDFTVAYLKTDIVMVVTEKEEKWKKWWAFMEAFDFEVWLLIPT 600
           YDGAVG+I IL  RF  VDFTV+YL+T+IVMVV EK  +WK+ WAF +AF+  +WLLIPT
Sbjct: 544 YDGAVGEIGILPNRFDIVDFTVSYLETEIVMVVKEKRSRWKQLWAFTDAFEVSMWLLIPT 603

Query: 601 MHLFVSSIIWLIERQNNDELKGFGNMLWFSISIIFYIHREPVKNGLARLVLGPWLFAILV 660
           MHLF+S  +WLIERQN++ELKGFGNMLWFS+SIIFY+HREPVKNGLARLVLGPWLF ILV
Sbjct: 604 MHLFISFAVWLIERQNSEELKGFGNMLWFSVSIIFYMHREPVKNGLARLVLGPWLFGILV 663

Query: 661 MTTSFSASLTSMMTISWSQPSVLDVETLKKMNATVGCNAESFIYNFLKNTLKFEPSKIVK 720
           +T SF++SLTSMMT+SW +PSVLDV  LK++NA VGCNA SFI ++LK TLKFEPSKI +
Sbjct: 664 VTASFTSSLTSMMTVSWYRPSVLDVVKLKEINAVVGCNARSFICDYLKKTLKFEPSKIKR 723

Query: 721 MNSIDDYPEAFKNDTIKAAFFISPHAEVFLAKNCRGYTKAVSSFKLGGIGFAFQKGSALA 779
           ++S+++YP+AF+++TIKAAFFISPHA+VFLAKNCRGYTK VSS+KL G+GFAF KGS LA
Sbjct: 724 IDSLNNYPKAFEDNTIKAAFFISPHADVFLAKNCRGYTKGVSSYKLSGVGFAFAKGSPLA 783

BLAST of Lag0004272 vs. TAIR 10
Match: AT5G11210.1 (glutamate receptor 2.5 )

HSP 1 Score: 280.8 bits (717), Expect = 3.4e-75
Identity = 211/690 (30.58%), Postives = 343/690 (49.71%), Query Frame = 0

Query: 141 LFDSFRSANIEIEHFLALSYSSSNQAEILIEKELEKFMNSQRNNRVFIVTQLSLKLVRLL 200
           L D+F+  N+ I +  A+S   S+     I+KEL K M      RVFIV  L   L   L
Sbjct: 115 LVDAFQEINVRIRYRSAISLHYSDDQ---IKKELYKLMTMP--TRVFIVHMLP-DLGSRL 174

Query: 201 FTKAKNMNMVGNGYAWIVSSDVFDHVVSLDSPSLLNKMEGVIGFRTYFDDTKKSFKSFET 260
           F+ AK ++M+  GY WIV++ + D +  +   SL+N M GV+G +TYF  +K+     E 
Sbjct: 175 FSIAKEIDMLSKGYVWIVTNGIADLMSIMGESSLVN-MHGVLGVKTYFAKSKE-LLHLEA 234

Query: 261 KLEKMYSLEYPQDKEPIKASIFTVRAYDASWAISKAIE---------------------- 320
           + +K +  E        + + F   AYDA+ A++ ++E                      
Sbjct: 235 RWQKRFGGE--------ELNNFACWAYDAATALAMSVEEIRHVNMSFNTTKEDTSRDDIG 294

Query: 321 ----KLGEKFSSHQLMEKILESNFEGLSGMVRFKNGMLISQAPTFQILKVVDGSLKDVAF 380
               +LG   S  +L++ +   +F+G++G  + KNG L  +A TF+I+ + +   + V F
Sbjct: 295 TDLDELGVALSGPKLLDALSTVSFKGVAGRFQLKNGKL--EATTFKIINIEESGERTVGF 354

Query: 381 WTPKLGFSESLVANNKELEFMENVAAVRHLSQTFSRELDGYGE-------------IRRL 440
           W  K+G  +SL            V  V H S+   R +   G+              ++L
Sbjct: 355 WKSKVGLVKSL-----------RVDKVSHSSRRL-RPIIWPGDTIFVPKGWEFPTNAKKL 414

Query: 441 KFAVPNQGACREFVNVNYHLNGTINNFTGFSIDLFKAVMHNI--TMSYHFVPF------- 500
           + AVP +     FV V    N  +   TGF ID+F  VM  +   +SY ++PF       
Sbjct: 415 RIAVPKKDGFNNFVEVTKDENTNVPTVTGFCIDVFNTVMSQMPYAVSYEYIPFDTPDGKP 474

Query: 501 HKTYNEMIDAVYNKTYDGAVGDITILAERFRRVDFTVAYLKTDIVMVVTEKEEKWKKWWA 560
             +Y+EM+  V+   +DGAVGD TILA R   VDF + Y +T IV +V  K+ K K  W 
Sbjct: 475 RGSYDEMVYNVFLGEFDGAVGDTTILANRSHYVDFALPYSETGIVFLVPVKDGKEKGEWV 534

Query: 561 FMEAFDFEVWLLIPTMHLFVSSIIWLIERQNNDELK------GFGNMLWFSISIIFYIHR 620
           F++    E+WL+     L++  ++W+ E Q ++E +         ++ +FS S +F+ HR
Sbjct: 535 FLKPLTKELWLVTAASFLYIGIMVWIFEYQADEEFREQMIIDKISSVFYFSFSTLFFAHR 594

Query: 621 EPVKNGLARLVLGPWLFAILVMTTSFSASLTSMMTISWSQPSVLDVETLKKMNATVGCNA 680
            P ++   R+++  W F +L++T S++A+LTSM+T+   +P+V  ++ L+K    +G   
Sbjct: 595 RPSESFFTRVLVVVWCFVLLILTQSYTATLTSMLTVQELRPTVRHMDDLRKSGVNIGYQT 654

Query: 681 ESFIYNFLKNTLKFEPSKIVKMNSIDDYPEAF----KNDTIKAAFFISPHAEVFLAKNCR 740
            SF +  LK  ++F+ S++   NS ++  E F     N  I AAF    + ++F+AK C 
Sbjct: 655 GSFTFERLKQ-MRFDESRLKTYNSPEEMRELFLHKSSNGGIDAAFDEVAYIKLFMAKYCS 714

Query: 741 GYTKAVSSFKLGGIGFAFQKGSALAAMVSESIANLAETNYTSEMEQK--LLASHC-PSTN 770
            Y+    +FK  G GFAF  GS L + +S  I N+ E +    +E K  L   HC  ST 
Sbjct: 715 EYSIIEPTFKADGFGFAFPLGSPLVSDISRQILNITEGDAMKAIENKWFLGEKHCLDSTT 773

BLAST of Lag0004272 vs. TAIR 10
Match: AT2G29100.1 (glutamate receptor 2.9 )

HSP 1 Score: 261.5 bits (667), Expect = 2.1e-69
Identity = 198/709 (27.93%), Postives = 344/709 (48.52%), Query Frame = 0

Query: 119 PFSMAAKVTVFHEINSFVSIRRLFDSFRSANIEI-----EHFLALSYSSSNQAEIL---- 178
           P+ + A +    ++ +  SI + F   R   I +     E F+   + +    E+     
Sbjct: 137 PYFVRATIDDSSQVRAIASIFKFFRWRRVVAIYVDNEFGEGFMPFLFDALQDVEVKRSVI 196

Query: 179 --------IEKELEKFMNSQRNNRVFIVTQLSLKLVRLLFTKAKNMNMVGNGYAWIVSSD 238
                   I+KEL K M  +R  RVF+V  +   L   +F  A+++ M+  GY W++++ 
Sbjct: 197 PPEAIDDEIQKELRKLM--ERQARVFVV-HMESSLALRVFQIARDIGMMEEGYVWLMTNG 256

Query: 239 VFDHVVSLDSPSLLNKMEGVIGFRTYFDDTKKSFKSFETKLEKMYSLEYPQDKEPIKASI 298
           +   +  +++   LN +EGV+G R++   +K+    F  + ++ +  E P  ++ +  ++
Sbjct: 257 MTHMMRHINNGRSLNTIEGVLGVRSHVPKSKE-LGDFRLRWKRTFEKENPSMRDDL--NV 316

Query: 299 FTVRAYDASWAISKAIEK-----------------------LGEKFSSHQLMEKILESNF 358
           F + AYD+  A++KA+EK                       +G       L +   E  F
Sbjct: 317 FALWAYDSITALAKAVEKANTKSLWYDNGSTLSKNRTDLGNVGVSLYGPSLQKAFSEVRF 376

Query: 359 EGLSGMVRFKNGMLISQAPTFQILKVVDGSLKDVAFWTPKLGFSESLVANNKELEFMENV 418
            GL+G  +  +G L  Q+P F+I+  V    + + FWTP+ G  ++  +N K L  +   
Sbjct: 377 NGLAGEFKLIDGQL--QSPKFEIINFVGNEERIIGFWTPRDGLMDATSSNKKTLGPVIWP 436

Query: 419 AAVRHLSQTFSRELDGYGEIRRLKFAVPNQGACREFVNVNYHLNGTINNFTGFSIDLFKA 478
              + + + +  E+ G    ++L+  VP +    +FV V  +        TG++I++F+A
Sbjct: 437 GKSKIVPKGW--EIPG----KKLRVGVPMKKGFFDFVKVTINPITNKKTPTGYAIEIFEA 496

Query: 479 VMHNI--TMSYHFVPFH--KTYNEMIDAVYNKTYDGAVGDITILAERFRRVDFTVAYLKT 538
            +  +   +   +V F     YN ++  VY+KT+D  VGDITI A R    DFT+ + ++
Sbjct: 497 ALKELPYLVIPEYVSFESPNNYNNLVYQVYDKTWDAVVGDITITANRSLYADFTLPFTES 556

Query: 539 DIVMVVTEKEEKWKKWWAFMEAFDFEVWLLIPTMHLFVSSIIWLIERQNNDELKG----- 598
            + M+V  ++ + K  W F+E +  E+W+      +F+  ++WL E + N + +G     
Sbjct: 557 GVSMMVPVRDNENKDTWVFLEPWSLELWVTTGCFFVFIGFVVWLFEHRVNTDFRGPPQYQ 616

Query: 599 FGNMLWFSISIIFYIHREPVKNGLARLVLGPWLFAILVMTTSFSASLTSMMTISWSQPSV 658
            G  LWFS S + + HRE V + LAR V+  W F +LV+T S++ASLTS +T+   QP+V
Sbjct: 617 IGTSLWFSFSTMVFAHRENVVSNLARFVVVVWCFVVLVLTQSYTASLTSFLTVQSLQPTV 676

Query: 659 LDVETLKKMNATVGCNAESFIYNFLKNTLKFEPSKIVKMNS---IDDYPEAFKNDTIKAA 718
            +V  L K    VG    +F+ + L   L F   ++   +S    DD     K+  I AA
Sbjct: 677 TNVNDLIKNRDCVGYQGGAFVKDILLG-LGFHEDQLKPFDSAKDADDLLSKGKSKGIAAA 736

Query: 719 FFISPHAEVFLAKNCRGYTKAVSSFKLGGIGFAFQKGSALAAMVSESIANLAETNYTSEM 772
           F    + +  L+++C  Y     +FK GG GFAF K S L    S +I NL + N T ++
Sbjct: 737 FDEVAYLKAILSQSCSKYVMVEPTFKTGGFGFAFPKNSPLTGEFSRAILNLTQNNVTQQI 796

BLAST of Lag0004272 vs. TAIR 10
Match: AT2G29120.1 (glutamate receptor 2.7 )

HSP 1 Score: 254.6 bits (649), Expect = 2.6e-67
Identity = 190/652 (29.14%), Postives = 311/652 (47.70%), Query Frame = 0

Query: 163 SNQAEILIEKELEKFMNSQRNNRVFIVTQLSLKLVRLLFTKAKNMNMVGNGYAWIVSSDV 222
           +N  +IL  KEL K M  Q   RVF+V  +   L    F KA+ + M+  GY W+++  V
Sbjct: 212 ANDDQIL--KELYKLMTMQ--TRVFVV-HMPPTLGFRFFQKAREIGMMEEGYVWLLTDGV 271

Query: 223 FDHVVSLDSPSLLNKMEGVIGFRTYFDDTKKSFKSFETKLEKMYSLEYPQDKEPIKASIF 282
            + + S +  S L  M+GV+G R++   +KK  K+F  + EKM    +P+     + +IF
Sbjct: 272 MNLLKSNERGSSLENMQGVLGVRSHIPKSKK-LKNFRLRWEKM----FPKKGNDEEMNIF 331

Query: 283 TVRAYDASWAISKAIEK-----------------------LGEKFSSHQLMEKILESNFE 342
            +RAYD+  A++ A+EK                       LG       L++ +    F 
Sbjct: 332 ALRAYDSITALAMAVEKTNIKSLRYDHPIASGNNKTNLGTLGVSRYGPSLLKALSNVRFN 391

Query: 343 GLSGMVRFKNGMLISQAPTFQILKVVDGSLKDVAFWTPKLGFSESLVANNKELEFMENVA 402
           GL+G     NG L  ++  F ++ ++    + +  W P  G   +   N   +   E + 
Sbjct: 392 GLAGEFELINGQL--ESSVFDVINIIGSEERIIGLWRPSNGIVNAKSKNTTSV-LGERLG 451

Query: 403 AVRHLSQTFSRELDGYGEI----RRLKFAVPNQGACREFVNVNYHLNGTINNFTGFSIDL 462
            V  +    S+++    +I    + L+  +P +    EFV+            TG+ I++
Sbjct: 452 PV--IWPGKSKDVPKGWQIPTNGKMLRVGIPVKKGFLEFVDAKIDPISNAMTPTGYCIEI 511

Query: 463 FKAVMHNITMS-----YHFVPFHKTYNEMIDAVYNKTYDGAVGDITILAERFRRVDFTVA 522
           F+AV+  +  S       F+   + Y+EM+  VY   YD  VGD+TI+A R   VDFT+ 
Sbjct: 512 FEAVLKKLPYSVIPKYIAFLSPDENYDEMVYQVYTGAYDAVVGDVTIVANRSLYVDFTLP 571

Query: 523 YLKTDIVMVVTEKEEKWKKWWAFMEAFDFEVWLLIPTMHLFVSSIIWLIERQNNDELKG- 582
           Y ++ + M+V  K+   K  W F+  +  ++W+      +F+  I+W++E + N + +G 
Sbjct: 572 YTESGVSMMVPLKDN--KNTWVFLRPWSLDLWVTTACFFVFIGFIVWILEHRVNTDFRGP 631

Query: 583 ----FGNMLWFSISIIFYIHREPVKNGLARLVLGPWLFAILVMTTSFSASLTSMMTISWS 642
                G   WF+ S + + HRE V + LAR V+  W F +LV+  S++A+LTS  T+   
Sbjct: 632 PHHQIGTSFWFAFSTMNFAHREKVVSNLARFVVLVWCFVVLVLIQSYTANLTSFFTVKLL 691

Query: 643 QPSVLDVETLKKMNATVGCNAESFIYNFLKNTLKFEPSKIVKMNSIDDYPEAFKNDTIKA 702
           QP+V + + L K N  +G    +F+   LK+   F+ S++    S  +  E F N TI A
Sbjct: 692 QPTVTNWKDLIKFNKNIGYQRGTFVRELLKSQ-GFDESQLKPFGSAVECDELFSNGTITA 751

Query: 703 AFFISPHAEVFLAKNCRGYTKAVSSFKLGGIGFAFQKGSALAAMVSESIANLAETNYTSE 762
           +F    + +V L++N   YT    SFK  G GF F K S L   VS +I N+ +      
Sbjct: 752 SFDEVAYIKVILSQNSSKYTMVEPSFKTAGFGFVFPKKSPLTDDVSRAILNVTQGEEMQH 811

Query: 763 MEQKLL--ASHCPSTNKA-DGLGLGPGPFMGLFLICGCIALLVLLYMGLQFM 775
           +E K     ++CP  N +     L    F GLFLI G  + L LL     F+
Sbjct: 812 IENKWFKKPNNCPDLNTSLSSNHLSLSSFWGLFLIAGIASFLALLIFVANFL 845

BLAST of Lag0004272 vs. TAIR 10
Match: AT2G29110.1 (glutamate receptor 2.8 )

HSP 1 Score: 240.0 bits (611), Expect = 6.7e-63
Identity = 192/678 (28.32%), Postives = 321/678 (47.35%), Query Frame = 0

Query: 141 LFDSFRSANIEIEHFLALSYSSSNQAEILIEKELEKFMNSQRNNRVFIVTQLSLKLVRLL 200
           LFD+ +   ++     ++  S +N  +IL  KEL K M   R  RVF+V  ++ +L   +
Sbjct: 185 LFDALQDVQVD----RSVIPSEANDDQIL--KELYKLMT--RQTRVFVV-HMASRLASRI 244

Query: 201 FTKAKNMNMVGNGYAWIVSSDVFDHVVSLDSPSLLNKMEGVIGFRTYFDDTKKSFKSFET 260
           F KA  + M+  GY W++++ +   +  +     LN ++GV+G R++   + K  + F  
Sbjct: 245 FEKATEIGMMEEGYVWLMTNGMTHMMRHIHHGRSLNTIDGVLGVRSHVPKS-KGLEDFRL 304

Query: 261 KLEKMYSLEYPQDKEPIKASIFTVRAYDASWAISKAIEK--------------------L 320
           + ++ +  E P  ++ +  SIF + AYD++ A++ A+EK                    L
Sbjct: 305 RWKRNFKKENPWLRDDL--SIFGLWAYDSTTALAMAVEKTNISSFPYNNASGSSNNMTDL 364

Query: 321 GEKFSSH---QLMEKILESNFEGLSGMVRFKNGMLISQAPTFQILKVVDGSLKDVAFWTP 380
           G    S     L+E + E  F GL+G     +  L  ++P F+I+  V    + V FWTP
Sbjct: 365 GTLHVSRYGPSLLEALSEIRFNGLAGRFNLIDRQL--ESPKFEIINFVGNEERIVGFWTP 424

Query: 381 KLGFSESLVANNKELEFMEN-----VAAVRHLSQTFSRELDGYGEIRRLKFAVPNQGACR 440
             G     V +NK   F        +   +        E+   G  +++K  VP +    
Sbjct: 425 SNGLVN--VNSNKTTSFTGERFGPLIWPGKSTIVPKGWEIPTNG--KKIKVGVPVKKGFF 484

Query: 441 EFVNVNYHLNGTINNFT---GFSIDLFKAVMHNITMS-----YHFVPFHKTYNEMIDAVY 500
            FV V   +   I N T   G++ID+F+A +  +  S     Y F      Y++++  V 
Sbjct: 485 NFVEV---ITDPITNITTPKGYAIDIFEAALKKLPYSVIPQYYRFESPDDDYDDLVYKVD 544

Query: 501 NKTYDGAVGDITILAERFRRVDFTVAYLKTDIVMVVTEKEEKWKKWWAFMEAFDFEVWLL 560
           N T D  VGD+TI A R    DFT+ Y ++ + M+V  ++ + K  W F++ +  ++W+ 
Sbjct: 545 NGTLDAVVGDVTITAYRSLYADFTLPYTESGVSMMVPVRDNENKNTWVFLKPWGLDLWVT 604

Query: 561 IPTMHLFVSSIIWLIERQNNDELKG-----FGNMLWFSISIIFYIHREPVKNGLARLVLG 620
                + +  ++WL E + N + +G      G   WFS S + + HRE V + LAR V+ 
Sbjct: 605 TACFFVLIGFVVWLFEHRVNTDFRGPPHHQIGTSFWFSFSTMVFAHREKVVSNLARFVVV 664

Query: 621 PWLFAILVMTTSFSASLTSMMTISWSQPSVLDVETLKKMNATVGCNAESFIYNFLKNTLK 680
            W F +LV+T S++A+LTS +T+   QP+ ++V+ L K    VG    +F+ +FL     
Sbjct: 665 VWCFVVLVLTQSYTANLTSFLTVQRFQPAAINVKDLIKNGDYVGYQHGAFVKDFLIKE-G 724

Query: 681 FEPSKIVKMNSIDDYPEAFKNDTIKAAFFISPHAEVFLAKNCRGYTKAVSSFKLGGIGFA 740
           F  SK+    S ++      N +I AAF    +    L++ C  Y     +FK  G GFA
Sbjct: 725 FNVSKLKPFGSSEECHALLSNGSISAAFDEVAYLRAILSQYCSKYAIVEPTFKTAGFGFA 784

Query: 741 FQKGSALAAMVSESIANLAETNYTSEMEQK--LLASHCPSTNKA-DGLGLGPGPFMGLFL 775
           F + S L   VS++I N+ + +    +E K  +  + CP    A     L    F GLFL
Sbjct: 785 FPRNSPLTGDVSKAILNVTQGDEMQHIENKWFMKQNDCPDPKTALSSNRLSLRSFWGLFL 840

BLAST of Lag0004272 vs. TAIR 10
Match: AT4G35290.2 (glutamate receptor 2 )

HSP 1 Score: 236.9 bits (603), Expect = 5.6e-62
Identity = 198/707 (28.01%), Postives = 341/707 (48.23%), Query Frame = 0

Query: 146 RSANIEIEHFLALSYSSSNQAEILIEKELEKFMNSQRNNRVFIVTQLSLKLVRLLFTKAK 205
           R   I  +  L L    ++  EI+   EL K    +  +RV IV     K  + +F +A+
Sbjct: 189 RRCKISYKAVLPLDVVITSPREII--NELVKIQGME--SRVIIVNTFP-KTGKKIFEEAQ 248

Query: 206 NMNMVGNGYAWIVS---SDVFDHVVSLDSPSLLNKMEGVIGFRTYFDDTKKSFKSFETKL 265
            + M+  GY WI +   + + D V  L + +    + GV+  R +  ++KK  K F  + 
Sbjct: 249 KLGMMEKGYVWIATTWLTSLLDSVNPLPAKT-AESLRGVLTLRIHTPNSKKK-KDFVARW 308

Query: 266 EKMYSLEYPQDKEPIKASIFTVRAYDASWAISKAIEKLGE-----KFSS----------- 325
            K+           +  +++ + AYD  W I++A+++L +      FSS           
Sbjct: 309 NKL-------SNGTVGLNVYGLYAYDTVWIIARAVKRLLDSRANISFSSDPKLTSMKGGG 368

Query: 326 -------------HQLMEKILESNFEGLSGMVRFKNGMLISQAPTFQILKVVDGSLKDVA 385
                         Q ++ I+ +N  G++G ++F     + Q P++ I+ VVD   + + 
Sbjct: 369 SLNLGALSIFDQGSQFLDYIVNTNMTGVTGQIQFLPDRSMIQ-PSYDIINVVDDGFRQIG 428

Query: 386 FWTPKLGFS--------ESLVANNKELEFMENVAAVRHLSQTFSRELDGYGEIRRLKFAV 445
           +W+   G S        + L   +   + + NV      S+T  R        RRL+  V
Sbjct: 429 YWSNHSGLSIIPPESLYKKLSNRSSSNQHLNNVTWPGGTSET-PRGWVFPNNGRRLRIGV 488

Query: 446 PNQGACREFVNVNYHLNGTINNFTGFSIDLFKAVMHNIT--MSYHFVPF-----HKTYNE 505
           P++ + +EFV+    L+G+ N   G++ID+F+A +  I+  + + FV F     +  +NE
Sbjct: 489 PDRASFKEFVS---RLDGS-NKVQGYAIDVFEAAVKLISYPVPHEFVLFGDGLKNPNFNE 548

Query: 506 MIDAVYNKTYDGAVGDITILAERFRRVDFTVAYLKTDIVMV--VTEKEEKWKKWWAFMEA 565
            ++ V    +D  VGDI I+ +R R VDFT  Y+++ +V+V  VT+  +     WAF+  
Sbjct: 549 FVNNVTIGVFDAVVGDIAIVTKRTRIVDFTQPYIESGLVVVAPVTKLND---TPWAFLRP 608

Query: 566 FDFEVWLLIPTMHLFVSSIIWLIERQNNDELKG-----FGNMLWFSISIIFYIHREPVKN 625
           F   +W +     L V S+IW++E + NDE +G        +LWFS S +F+ HRE   +
Sbjct: 609 FTPPMWAVTAAFFLIVGSVIWILEHRINDEFRGPPRKQIVTILWFSFSTMFFSHRENTVS 668

Query: 626 GLARLVLGPWLFAILVMTTSFSASLTSMMTISWSQPSVLDVETLKKMNATVGCNAESFIY 685
            L R VL  WLF +L++T+S++ASLTS++T+      +  V+TL   +  VG    S+  
Sbjct: 669 TLGRAVLLIWLFVVLIITSSYTASLTSILTVQQLNSPIRGVDTLISSSGRVGFQVGSYAE 728

Query: 686 NFLKNTLKFEPSKIVKMNSIDDYPEAFKNDTIKAAFFISPHAEVFLAKNCRGYTKAVSSF 745
           N++ + L    S++V + S  +Y  A +N T+ A     P+ ++FL++ C G+      F
Sbjct: 729 NYMIDELNIARSRLVPLGSPKEYAAALQNGTVAAIVDERPYVDLFLSEFC-GFAIRGQEF 788

Query: 746 KLGGIGFAFQKGSALAAMVSESIANLAETNYTSEMEQKLLA-SHCPSTNKA----DGLGL 794
              G GFAF + S LA  +S +I  L+ET    ++  K L+ S+C + N +    D   L
Sbjct: 789 TRSGWGFAFPRDSPLAIDMSTAILGLSETGQLQKIHDKWLSRSNCSNLNGSVSDEDSEQL 848