Lag0004255 (gene) Sponge gourd (AG‐4) v1

BLAST of Lag0004255 vs. NCBI nr
Match: XP_038885464.1 (probable E3 ubiquitin-protein ligase RHG1A [Benincasa hispida] >XP_038885465.1 probable E3 ubiquitin-protein ligase RHG1A [Benincasa hispida] >XP_038885466.1 probable E3 ubiquitin-protein ligase RHG1A [Benincasa hispida] >XP_038885467.1 probable E3 ubiquitin-protein ligase RHG1A [Benincasa hispida])

HSP 1 Score: 1298.9 bits (3360), Expect = 0.0e+00
Identity = 662/713 (92.85%), Postives = 689/713 (96.63%), Query Frame = 0

Query: 1   MQGQRSSVGSLSETINFEHGSSSNNPGNQPVYWNNMWNPAENRIPDYLLPTSDINTGYVS 60
           MQGQRSSVGSLSETINFEHGSSSNNPGNQPVYWNNMWNPAENRI DYLLPTSDIN+GYVS
Sbjct: 1   MQGQRSSVGSLSETINFEHGSSSNNPGNQPVYWNNMWNPAENRIQDYLLPTSDINSGYVS 60

Query: 61  SVSHEQRSLSRWSLGEPSSCDMQTEALPDEQKAELGWSSVTRDADGPVTDNRLCEPSNNP 120
           SVSHEQRSLSRWSLGEPSSCDMQTE LPDEQKAE+GWSS+TRDADGPVT+NRLCE SNNP
Sbjct: 61  SVSHEQRSLSRWSLGEPSSCDMQTEVLPDEQKAEIGWSSLTRDADGPVTENRLCESSNNP 120

Query: 121 SLGHVNLSPLIIQ--NSNSNAIPHNLNLNASFASHGGDNSRVNEGSNVYKSSGSEEGRIL 180
           SLGHVNLSPLIIQ  NSNSNAIPHNLNLNASFASHGGDNSRVNEG+NVYKSSGSEEGRIL
Sbjct: 121 SLGHVNLSPLIIQNSNSNSNAIPHNLNLNASFASHGGDNSRVNEGTNVYKSSGSEEGRIL 180

Query: 181 CSSASDPLLLPSGNSGLPVVANDGRAGSSLDGRRAPCKRKAIEGSVAQSSISGSSNYSQH 240
           CSS SDPLLLPSGNSGLPVVAND RAGSSLDGRRAPCKRK+IEGSVAQSS+SGSSNYSQH
Sbjct: 181 CSSTSDPLLLPSGNSGLPVVANDSRAGSSLDGRRAPCKRKSIEGSVAQSSLSGSSNYSQH 240

Query: 241 IESGAETPLPVFPGRYNTGNRLTIPAPPARMNQASLRGAGELTSNSFSESIVAESSDSSQ 300
           IESG+E  LPVFPGRYNTG+RL+IP  PARMNQA +RGAGELTSN+FSESIVAESSDSSQ
Sbjct: 241 IESGSEPHLPVFPGRYNTGSRLSIPPAPARMNQAPVRGAGELTSNNFSESIVAESSDSSQ 300

Query: 301 RNYRIRISSSNAQDSFSSAGTTVRHPGASSSQLPARLLPAEHVLDLRPAPAVDNIPAQGQ 360
           RNYRIRISSSNAQDSF+SAGT VRHPG  SSQLPARLLP EHVLDLRPAPAVDN+ AQGQ
Sbjct: 301 RNYRIRISSSNAQDSFASAGTAVRHPGPPSSQLPARLLPTEHVLDLRPAPAVDNVSAQGQ 360

Query: 361 PIMIHVPALPRNLQPYRWNGSSTSRSGSSSSSAGERQVVQREEVRPSNMGRNVSEHPMFV 420
           PIMIHVPALPR+LQPYRWNGSSTSRSGSSS+S GERQVVQREEVRPSNMGRNVSEHPMFV
Sbjct: 361 PIMIHVPALPRSLQPYRWNGSSTSRSGSSSTSTGERQVVQREEVRPSNMGRNVSEHPMFV 420

Query: 421 PANELRNLVRNPSSRGITSSNLSIPGNVASTSRVGSSSGVHPSAGPWMPPENSPTQFPRR 480
           PANELRNLVRNPS+RG+ SSNL+IPGNVASTSR+GSSSGVHPS+GPW+PPE+SPTQFPRR
Sbjct: 421 PANELRNLVRNPSNRGLASSNLAIPGNVASTSRIGSSSGVHPSSGPWIPPESSPTQFPRR 480

Query: 481 LTEYVRRQLFSSATNESGGRTSNYSQRSGSTSAQDMVLSSGSGRRGHGLLQPRSALWMER 540
           LTEYVRRQLF+SATNESGGRTSNYSQRSGSTSAQDMVLSSGSGRRGH LLQPRSALWMER
Sbjct: 481 LTEYVRRQLFASATNESGGRTSNYSQRSGSTSAQDMVLSSGSGRRGHHLLQPRSALWMER 540

Query: 541 RGDGGLGLPYSLRTLAASGEGSDNNRLVSEQIRNVLGLVRRGESLRVEDVMILDQSLFFG 600
           RGDGGLGLPYSLRTLA SGEGSDNNRLVSE IRNVLGLVRRGESLRVEDVMILDQSLFFG
Sbjct: 541 RGDGGLGLPYSLRTLATSGEGSDNNRLVSEHIRNVLGLVRRGESLRVEDVMILDQSLFFG 600

Query: 601 MADIYDRHRDMRLDVDNMSYEELLALEERIGNVNTGLNEETIVARLKQKKHINATESQVE 660
           MADIYDRHRDMRLDVDNMSYEELLALEERIGNVNTGLNEETIVARLKQKKH+NA +SQVE
Sbjct: 601 MADIYDRHRDMRLDVDNMSYEELLALEERIGNVNTGLNEETIVARLKQKKHVNAVDSQVE 660

Query: 661 EEPCCVCQEEYIEGEDIGTLECGHDFHTHCIKQWLMQKNLCPICKTTGLASRE 712
           EEPCCVCQEEY+EGEDIGTLECGHDFHT CIKQWLMQKNLCPICKTTGLASRE
Sbjct: 661 EEPCCVCQEEYVEGEDIGTLECGHDFHTACIKQWLMQKNLCPICKTTGLASRE 713

BLAST of Lag0004255 vs. NCBI nr
Match: XP_016900001.1 (PREDICTED: probable E3 ubiquitin-protein ligase HIP1 [Cucumis melo] >XP_016900002.1 PREDICTED: probable E3 ubiquitin-protein ligase HIP1 [Cucumis melo] >ADN33722.1 zinc finger protein binding protein [Cucumis melo subsp. melo])

HSP 1 Score: 1286.6 bits (3328), Expect = 0.0e+00
Identity = 652/713 (91.44%), Postives = 687/713 (96.35%), Query Frame = 0

Query: 1   MQGQRSSVGSLSETINFEHGSSSNNPGNQPVYWNNMWNPAENRIPDYLLPTSDINTGYVS 60
           MQGQRSSVGSLSETINFEHGSSSNNPGNQPVYWNN+WNPAENRIPDYLLPTSD+N+GYV+
Sbjct: 1   MQGQRSSVGSLSETINFEHGSSSNNPGNQPVYWNNVWNPAENRIPDYLLPTSDVNSGYVN 60

Query: 61  SVSHEQRSLSRWSLGEPSSCDMQTEALPDEQKAELGWSSVTRDADGPVTDNRLCEPSNNP 120
           SVSHEQRSLSRWSLGEPSSCDMQTE LPDEQKAE+GWSS+  DADGPVT+NRLCEPSNNP
Sbjct: 61  SVSHEQRSLSRWSLGEPSSCDMQTEMLPDEQKAEIGWSSMASDADGPVTENRLCEPSNNP 120

Query: 121 SLGHVNLSPLIIQ--NSNSNAIPHNLNLNASFASHGGDNSRVNEGSNVYKSSGSEEGRIL 180
           SLGHVNLSPLIIQ  NSNSNAIPHNLNLN+SF SHGGDNSRVNEG+NVYKSSG EEGRIL
Sbjct: 121 SLGHVNLSPLIIQNSNSNSNAIPHNLNLNSSFVSHGGDNSRVNEGTNVYKSSGPEEGRIL 180

Query: 181 CSSASDPLLLPSGNSGLPVVANDGRAGSSLDGRRAPCKRKAIEGSVAQSSISGSSNYSQH 240
           CS+ASDPLLLPSGNSGLPVV NDGR GSSLDGRRAPCKRK+IEG+VAQSS+SGSSNYSQH
Sbjct: 181 CSNASDPLLLPSGNSGLPVVGNDGRPGSSLDGRRAPCKRKSIEGNVAQSSLSGSSNYSQH 240

Query: 241 IESGAETPLPVFPGRYNTGNRLTIPAPPARMNQASLRGAGELTSNSFSESIVAESSDSSQ 300
           IESG+E  LPVFPGRYNTG+R++IP PPARMNQA +RGAGELTSN+FSESIVAESSDSSQ
Sbjct: 241 IESGSEAHLPVFPGRYNTGSRISIPPPPARMNQAPVRGAGELTSNNFSESIVAESSDSSQ 300

Query: 301 RNYRIRISSSNAQDSFSSAGTTVRHPGASSSQLPARLLPAEHVLDLRPAPAVDNIPAQGQ 360
           RNYRIRISSSNAQDSF+SAGT VRHPG  SSQLPARLLPAEH+LDLRPAPAVDN+ AQGQ
Sbjct: 301 RNYRIRISSSNAQDSFASAGTAVRHPGPPSSQLPARLLPAEHILDLRPAPAVDNVSAQGQ 360

Query: 361 PIMIHVPALPRNLQPYRWNGSSTSRSGSSSSSAGERQVVQREEVRPSNMGRNVSEHPMFV 420
           PIMIHVPALPRNLQPYRWNGSSTSRSGSSSSSAGERQVVQREEVRPS MGRNVSEHPMF+
Sbjct: 361 PIMIHVPALPRNLQPYRWNGSSTSRSGSSSSSAGERQVVQREEVRPSIMGRNVSEHPMFL 420

Query: 421 PANELRNLVRNPSSRGITSSNLSIPGNVASTSRVGSSSGVHPSAGPWMPPENSPTQFPRR 480
           PANELR LVR+PSSR + SSNL+IPGNVASTSRVGSSSGVHPS+GPW+PPE+SPTQFPRR
Sbjct: 421 PANELRGLVRSPSSRALASSNLAIPGNVASTSRVGSSSGVHPSSGPWIPPESSPTQFPRR 480

Query: 481 LTEYVRRQLFSSATNESGGRTSNYSQRSGSTSAQDMVLSSGSGRRGHGLLQPRSALWMER 540
           LTEYVRRQLF+SATNESGGRTSNYSQRSGSTSAQ+MVLSSGSGRRGH LLQPRSALWMER
Sbjct: 481 LTEYVRRQLFASATNESGGRTSNYSQRSGSTSAQEMVLSSGSGRRGHHLLQPRSALWMER 540

Query: 541 RGDGGLGLPYSLRTLAASGEGSDNNRLVSEQIRNVLGLVRRGESLRVEDVMILDQSLFFG 600
           RGDGGLGLPYSLRTLA+S EGSDNNRLVSE +RNVLGLVRRGESLRVEDVMILDQSLFFG
Sbjct: 541 RGDGGLGLPYSLRTLASSAEGSDNNRLVSEHLRNVLGLVRRGESLRVEDVMILDQSLFFG 600

Query: 601 MADIYDRHRDMRLDVDNMSYEELLALEERIGNVNTGLNEETIVARLKQKKHINATESQVE 660
           MADIYDRHRDMRLDVDNMSYEELLALEERIGNVNTGLNEETIVARLKQKKH+NA ESQVE
Sbjct: 601 MADIYDRHRDMRLDVDNMSYEELLALEERIGNVNTGLNEETIVARLKQKKHVNAVESQVE 660

Query: 661 EEPCCVCQEEYIEGEDIGTLECGHDFHTHCIKQWLMQKNLCPICKTTGLASRE 712
           EEPCCVCQEEY+EGEDIGTLECGHDFHT CIKQWLMQKNLCPICKTTGLASRE
Sbjct: 661 EEPCCVCQEEYVEGEDIGTLECGHDFHTACIKQWLMQKNLCPICKTTGLASRE 713

BLAST of Lag0004255 vs. NCBI nr
Match: XP_022951413.1 (probable E3 ubiquitin-protein ligase RHG1A isoform X1 [Cucurbita moschata] >XP_022951414.1 probable E3 ubiquitin-protein ligase RHG1A isoform X1 [Cucurbita moschata] >XP_022951415.1 probable E3 ubiquitin-protein ligase RHG1A isoform X1 [Cucurbita moschata] >XP_022951416.1 probable E3 ubiquitin-protein ligase RHG1A isoform X1 [Cucurbita moschata] >KAG7020582.1 putative E3 ubiquitin-protein ligase RHG1A, partial [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 1284.2 bits (3322), Expect = 0.0e+00
Identity = 653/711 (91.84%), Postives = 681/711 (95.78%), Query Frame = 0

Query: 1   MQGQRSSVGSLSETINFEHGSSSNNPGNQPVYWNNMWNPAENRIPDYLLPTSDINTGYVS 60
           MQGQRSSVGSLSETINFEHGSSSNNPG+QPVYWNNMWNPAENRIPDYLLPT+DINTGYVS
Sbjct: 1   MQGQRSSVGSLSETINFEHGSSSNNPGSQPVYWNNMWNPAENRIPDYLLPTTDINTGYVS 60

Query: 61  SVSHEQRSLSRWSLGEPSSCDMQTEALPDEQKAELGWSSVTRDADGPVTDNRLCEPSNNP 120
           SVSHEQR+LSRWSLGEPSSCDMQTEAL DEQK E+GWSSV RDADGPVT+ RLCEPSNNP
Sbjct: 61  SVSHEQRNLSRWSLGEPSSCDMQTEALSDEQKGEVGWSSVARDADGPVTETRLCEPSNNP 120

Query: 121 SLGHVNLSPLIIQNSNSNAIPHNLNLNASFASHGGDNSRVNEGSNVYKSSGSEEGRILCS 180
           +LGHVN SPLIIQNSNSNA+PH+LNLNASFASHGG++SRVNEGSN+YKSSGSEEGR LCS
Sbjct: 121 TLGHVNFSPLIIQNSNSNALPHSLNLNASFASHGGESSRVNEGSNLYKSSGSEEGRALCS 180

Query: 181 SASDPLLLPSGNSGLPVVANDGRAGSSLDGRRAPCKRKAIEGSVAQSSISGSSNYSQHIE 240
           SASDPLLLPSGNSGLPVVANDGRAGSSLDGRRAPCKRKAIEGSV Q S+SGSSNY+QHIE
Sbjct: 181 SASDPLLLPSGNSGLPVVANDGRAGSSLDGRRAPCKRKAIEGSVTQPSLSGSSNYAQHIE 240

Query: 241 SGAETPLPVFPGRYNTGNRLTIPAPPARMNQASLRGAGELTSNSFSESIVAESSDSSQRN 300
           SGAE  LPVFPGRYNTG+RLTIPAPPARMNQA +RGAGE+TSNSFSES+VAESSDSSQRN
Sbjct: 241 SGAEPTLPVFPGRYNTGSRLTIPAPPARMNQAPVRGAGEMTSNSFSESMVAESSDSSQRN 300

Query: 301 YRIRISSSNAQDSFSSAGTTVRHPGASSSQLPARLLPAEHVLDLRPAPAVDNIPAQGQPI 360
           YRIRISSSNAQDSF+  G TVRHPGASSSQLPAR+LPAEHVLDLRPAPAVDNI AQGQP+
Sbjct: 301 YRIRISSSNAQDSFAPTGMTVRHPGASSSQLPARILPAEHVLDLRPAPAVDNISAQGQPL 360

Query: 361 MIHVPALPRNLQPYRWNGSSTSRSGSSSSSAGERQVVQREEVRPSNMGRNVSEHPMFVPA 420
           MIHVPALPR+LQPYRWNGSSTSRSG SSSSAGERQVVQREEVRPSNMGRNVSEHPMFVPA
Sbjct: 361 MIHVPALPRSLQPYRWNGSSTSRSGGSSSSAGERQVVQREEVRPSNMGRNVSEHPMFVPA 420

Query: 421 NELRNLVRNPSSRGITSSNLSIPGNVASTSRVGSSSGVHPSAGPWMPPENSPTQFPRRLT 480
           NELRNLVRNPSSRG+ SSNLSIPG VASTSRVGSSSGVHP +GPWMPPENSPTQFPRRLT
Sbjct: 421 NELRNLVRNPSSRGLASSNLSIPGTVASTSRVGSSSGVHPPSGPWMPPENSPTQFPRRLT 480

Query: 481 EYVRRQLFSSATNESGGRTSNYSQRSGSTSAQDMVLSSGSGRRGHGLLQPRSALWMERRG 540
           EYVRRQLFSSATNESGGRTSNYSQRSGSTSAQDMVLSSGSGRRGH LLQPRSALWMERRG
Sbjct: 481 EYVRRQLFSSATNESGGRTSNYSQRSGSTSAQDMVLSSGSGRRGHHLLQPRSALWMERRG 540

Query: 541 DGGLGLPYSLRTLAASGEGSDNNRLVSEQIRNVLGLVRRGESLRVEDVMILDQSLFFGMA 600
           D GLGLPYSLRTLA SGEGSDNNRLVSEQIRNVLGLVRRGESLRVEDVMILDQSLFFGMA
Sbjct: 541 DTGLGLPYSLRTLATSGEGSDNNRLVSEQIRNVLGLVRRGESLRVEDVMILDQSLFFGMA 600

Query: 601 DIYDRHRDMRLDVDNMSYEELLALEERIGNVNTGLNEETIVARLKQKKHINATESQVEEE 660
           DIYDRHRDMRLDVDNMSYEELLALEERIGNVNTGL+EETIVARLK+ KH+NA E QVEEE
Sbjct: 601 DIYDRHRDMRLDVDNMSYEELLALEERIGNVNTGLSEETIVARLKRTKHVNAPEPQVEEE 660

Query: 661 PCCVCQEEYIEGEDIGTLECGHDFHTHCIKQWLMQKNLCPICKTTGLASRE 712
           PCCVCQEEY EGEDIG L+CGH FHT CIKQWLMQKN+CPICKTTG ASRE
Sbjct: 661 PCCVCQEEYKEGEDIGMLDCGHKFHTQCIKQWLMQKNICPICKTTGFASRE 711

BLAST of Lag0004255 vs. NCBI nr
Match: XP_023002037.1 (probable E3 ubiquitin-protein ligase RHG1A isoform X1 [Cucurbita maxima] >XP_023002038.1 probable E3 ubiquitin-protein ligase RHG1A isoform X1 [Cucurbita maxima] >XP_023002039.1 probable E3 ubiquitin-protein ligase RHG1A isoform X1 [Cucurbita maxima])

HSP 1 Score: 1282.7 bits (3318), Expect = 0.0e+00
Identity = 650/711 (91.42%), Postives = 683/711 (96.06%), Query Frame = 0

Query: 1   MQGQRSSVGSLSETINFEHGSSSNNPGNQPVYWNNMWNPAENRIPDYLLPTSDINTGYVS 60
           MQGQRSSVGSLSETINFEHGSSSNNPG+QPVYWNNMWNPAENRIPDYLLPT+DINTGYV+
Sbjct: 1   MQGQRSSVGSLSETINFEHGSSSNNPGSQPVYWNNMWNPAENRIPDYLLPTTDINTGYVT 60

Query: 61  SVSHEQRSLSRWSLGEPSSCDMQTEALPDEQKAELGWSSVTRDADGPVTDNRLCEPSNNP 120
           +VSHEQR+LSRWSLGEPSSCDMQTEAL DEQK E+GWSSV+RDADGPVT+ RLCEPSNNP
Sbjct: 61  TVSHEQRNLSRWSLGEPSSCDMQTEALSDEQKGEVGWSSVSRDADGPVTETRLCEPSNNP 120

Query: 121 SLGHVNLSPLIIQNSNSNAIPHNLNLNASFASHGGDNSRVNEGSNVYKSSGSEEGRILCS 180
           +LGHVN SP+IIQNSNSNA+PH+LNLNASFASHGG++SRVNEGSN+YKS+GSEEGR LCS
Sbjct: 121 TLGHVNFSPMIIQNSNSNALPHSLNLNASFASHGGESSRVNEGSNLYKSNGSEEGRALCS 180

Query: 181 SASDPLLLPSGNSGLPVVANDGRAGSSLDGRRAPCKRKAIEGSVAQSSISGSSNYSQHIE 240
           SASDPLLLPSGNSGLPVVANDGRAGSSLDGRRAPCKRKAIEGSV Q S+SGSSNY+QHIE
Sbjct: 181 SASDPLLLPSGNSGLPVVANDGRAGSSLDGRRAPCKRKAIEGSVTQPSLSGSSNYAQHIE 240

Query: 241 SGAETPLPVFPGRYNTGNRLTIPAPPARMNQASLRGAGELTSNSFSESIVAESSDSSQRN 300
           SGAE  LPVFPGRYNTG+RLTIPAPPARMNQA +RGAGE+TSNSFSES+VAESSDSSQRN
Sbjct: 241 SGAEPTLPVFPGRYNTGSRLTIPAPPARMNQAPVRGAGEMTSNSFSESMVAESSDSSQRN 300

Query: 301 YRIRISSSNAQDSFSSAGTTVRHPGASSSQLPARLLPAEHVLDLRPAPAVDNIPAQGQPI 360
           YRIRISSSNAQDSF+  G TVRHPGASSSQLPAR+LPAEHVLDLRPAPAVDNI AQGQP+
Sbjct: 301 YRIRISSSNAQDSFAPTGMTVRHPGASSSQLPARILPAEHVLDLRPAPAVDNISAQGQPL 360

Query: 361 MIHVPALPRNLQPYRWNGSSTSRSGSSSSSAGERQVVQREEVRPSNMGRNVSEHPMFVPA 420
           MIHVPALPR+LQPYRWNGSSTSRSGSSSSSAGERQVVQREEVRPSNMGRNVSEHPMFVPA
Sbjct: 361 MIHVPALPRSLQPYRWNGSSTSRSGSSSSSAGERQVVQREEVRPSNMGRNVSEHPMFVPA 420

Query: 421 NELRNLVRNPSSRGITSSNLSIPGNVASTSRVGSSSGVHPSAGPWMPPENSPTQFPRRLT 480
           NELRNLVRNPSSRG+ SSNLSIPG VASTSRVGSSSGVHP +GPWMPPENSPTQFPRRLT
Sbjct: 421 NELRNLVRNPSSRGLASSNLSIPGTVASTSRVGSSSGVHPPSGPWMPPENSPTQFPRRLT 480

Query: 481 EYVRRQLFSSATNESGGRTSNYSQRSGSTSAQDMVLSSGSGRRGHGLLQPRSALWMERRG 540
           EYVRRQLFSSATNESGGRTSNYSQRSGSTSAQDMVLSSGSGRRGH LLQPRSALWMERRG
Sbjct: 481 EYVRRQLFSSATNESGGRTSNYSQRSGSTSAQDMVLSSGSGRRGHHLLQPRSALWMERRG 540

Query: 541 DGGLGLPYSLRTLAASGEGSDNNRLVSEQIRNVLGLVRRGESLRVEDVMILDQSLFFGMA 600
           D GLGLPYSLRTLA SGEGSDNNRLVSEQIRNVLGLVRRGESLRVEDVMILDQSLFFGMA
Sbjct: 541 DSGLGLPYSLRTLATSGEGSDNNRLVSEQIRNVLGLVRRGESLRVEDVMILDQSLFFGMA 600

Query: 601 DIYDRHRDMRLDVDNMSYEELLALEERIGNVNTGLNEETIVARLKQKKHINATESQVEEE 660
           DIYDRHRDMRLDVDNMSYEELLALEERIGNVNTGL+EETIVARLK+ KH+NA E QVEEE
Sbjct: 601 DIYDRHRDMRLDVDNMSYEELLALEERIGNVNTGLSEETIVARLKRTKHVNAPEPQVEEE 660

Query: 661 PCCVCQEEYIEGEDIGTLECGHDFHTHCIKQWLMQKNLCPICKTTGLASRE 712
           PCCVCQEEY EGEDIG L+CGH FHT CIKQWLMQKN+CPICKTTG ASRE
Sbjct: 661 PCCVCQEEYKEGEDIGMLDCGHKFHTQCIKQWLMQKNICPICKTTGFASRE 711

BLAST of Lag0004255 vs. NCBI nr
Match: XP_023536901.1 (probable E3 ubiquitin-protein ligase RHG1A isoform X1 [Cucurbita pepo subsp. pepo] >XP_023536903.1 probable E3 ubiquitin-protein ligase RHG1A isoform X1 [Cucurbita pepo subsp. pepo] >XP_023536904.1 probable E3 ubiquitin-protein ligase RHG1A isoform X1 [Cucurbita pepo subsp. pepo] >XP_023536905.1 probable E3 ubiquitin-protein ligase RHG1A isoform X1 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 1281.9 bits (3316), Expect = 0.0e+00
Identity = 651/711 (91.56%), Postives = 680/711 (95.64%), Query Frame = 0

Query: 1   MQGQRSSVGSLSETINFEHGSSSNNPGNQPVYWNNMWNPAENRIPDYLLPTSDINTGYVS 60
           MQGQRSSVGSLSETINFEHGSSSNNPG+QP YWNNMWNPAENRIPDYLLPT+DINTGYVS
Sbjct: 1   MQGQRSSVGSLSETINFEHGSSSNNPGSQPAYWNNMWNPAENRIPDYLLPTTDINTGYVS 60

Query: 61  SVSHEQRSLSRWSLGEPSSCDMQTEALPDEQKAELGWSSVTRDADGPVTDNRLCEPSNNP 120
           SVSHEQR+LSRWSLGEPSSCDMQTEAL DEQK E+GWSSV RDADGPVT+ RLCEPSNNP
Sbjct: 61  SVSHEQRNLSRWSLGEPSSCDMQTEALSDEQKGEVGWSSVARDADGPVTETRLCEPSNNP 120

Query: 121 SLGHVNLSPLIIQNSNSNAIPHNLNLNASFASHGGDNSRVNEGSNVYKSSGSEEGRILCS 180
           SLGHVN SPLIIQNSNSNA+PH+LNLNASFASHGG++SRVNEGSN+YKSSGSEEGR LCS
Sbjct: 121 SLGHVNFSPLIIQNSNSNALPHSLNLNASFASHGGESSRVNEGSNLYKSSGSEEGRALCS 180

Query: 181 SASDPLLLPSGNSGLPVVANDGRAGSSLDGRRAPCKRKAIEGSVAQSSISGSSNYSQHIE 240
           SASDPLLLPSGNSGLPVVANDGRAGSS+DGRRAPCKRKAIEGSV Q S+SGSSNY+QHIE
Sbjct: 181 SASDPLLLPSGNSGLPVVANDGRAGSSMDGRRAPCKRKAIEGSVTQPSLSGSSNYAQHIE 240

Query: 241 SGAETPLPVFPGRYNTGNRLTIPAPPARMNQASLRGAGELTSNSFSESIVAESSDSSQRN 300
           SGAE  LPVFPGRYNTG+RLTIPAPPARMNQA +RGAGE+TSNSFSES+VAESSDSSQRN
Sbjct: 241 SGAEPTLPVFPGRYNTGSRLTIPAPPARMNQAPVRGAGEMTSNSFSESMVAESSDSSQRN 300

Query: 301 YRIRISSSNAQDSFSSAGTTVRHPGASSSQLPARLLPAEHVLDLRPAPAVDNIPAQGQPI 360
           YRIRISSSNAQDSF+  G TVRHPGASSSQLPAR+LPAEHVLDLRPAPA+DNI AQGQP+
Sbjct: 301 YRIRISSSNAQDSFAPTGMTVRHPGASSSQLPARILPAEHVLDLRPAPALDNISAQGQPL 360

Query: 361 MIHVPALPRNLQPYRWNGSSTSRSGSSSSSAGERQVVQREEVRPSNMGRNVSEHPMFVPA 420
           MIHVPALPR+LQPYRWNGSSTSRSG SSSSAGERQVVQREEVRPSNMGRNVSEHPMFVPA
Sbjct: 361 MIHVPALPRSLQPYRWNGSSTSRSGGSSSSAGERQVVQREEVRPSNMGRNVSEHPMFVPA 420

Query: 421 NELRNLVRNPSSRGITSSNLSIPGNVASTSRVGSSSGVHPSAGPWMPPENSPTQFPRRLT 480
           NELRNLVRNPSSRG+ SSNLSIPG VASTSRVGSSSGVHP +GPWMPPENSPTQFPRRLT
Sbjct: 421 NELRNLVRNPSSRGLASSNLSIPGTVASTSRVGSSSGVHPPSGPWMPPENSPTQFPRRLT 480

Query: 481 EYVRRQLFSSATNESGGRTSNYSQRSGSTSAQDMVLSSGSGRRGHGLLQPRSALWMERRG 540
           EYVRRQLFSSATNESGGRTSNYSQRSGSTSAQDMVLSSGSGRRGH LLQPRSALWMERRG
Sbjct: 481 EYVRRQLFSSATNESGGRTSNYSQRSGSTSAQDMVLSSGSGRRGHHLLQPRSALWMERRG 540

Query: 541 DGGLGLPYSLRTLAASGEGSDNNRLVSEQIRNVLGLVRRGESLRVEDVMILDQSLFFGMA 600
           D GLGLPYSLRTLA SGEGSDNNRLVSEQIRNVLGLVRRGESLRVEDVMILDQSLFFGMA
Sbjct: 541 DTGLGLPYSLRTLATSGEGSDNNRLVSEQIRNVLGLVRRGESLRVEDVMILDQSLFFGMA 600

Query: 601 DIYDRHRDMRLDVDNMSYEELLALEERIGNVNTGLNEETIVARLKQKKHINATESQVEEE 660
           DIYDRHRDMRLDVDNMSYEELLALEERIGNVNTGL+EETIVARLK+ KH+NA E QVEEE
Sbjct: 601 DIYDRHRDMRLDVDNMSYEELLALEERIGNVNTGLSEETIVARLKRTKHVNAPEPQVEEE 660

Query: 661 PCCVCQEEYIEGEDIGTLECGHDFHTHCIKQWLMQKNLCPICKTTGLASRE 712
           PCCVCQEEY EGEDIG L+CGH FHT CIKQWLMQKN+CPICKTTG ASRE
Sbjct: 661 PCCVCQEEYKEGEDIGMLDCGHKFHTQCIKQWLMQKNICPICKTTGFASRE 711

BLAST of Lag0004255 vs. ExPASy Swiss-Prot
Match: Q9FMM4 (Probable E3 ubiquitin-protein ligase RHG1A OS=Arabidopsis thaliana OX=3702 GN=RHG1A PE=2 SV=1)

HSP 1 Score: 386.7 bits (992), Expect = 5.6e-106
Identity = 296/730 (40.55%), Postives = 408/730 (55.89%), Query Frame = 0

Query: 1   MQGQRSSVGSLSETINFEHGSSSNNPGNQPVYWNNMWNPAENRIPDYLLPTSDINTGYVS 60
           MQG+R+S+G LSE +NFEHGSSS+N      +W N+ +  +N + DY++  S+ NT   +
Sbjct: 1   MQGERASLGYLSEALNFEHGSSSSN--GVIDHWENIHSLGDNDLQDYMIANSESNTSLAN 60

Query: 61  SVSHEQRSLSRWSLGEPSSCDMQTEALP-DEQKAELGWSSVTRDADGPVTDNRLCEPSNN 120
           SV HEQ+ L R+SLGE SS   + EA   +EQ+ E      TR  DG    N +      
Sbjct: 61  SVYHEQQGLRRFSLGEASSSGTKDEASSHNEQRME------TRCFDG--RGNEI------ 120

Query: 121 PSLGHVNLSPLIIQNSNSNAIPHNLNLNASFASHGGDNSRVNEGSNVYKS-SGSEEGRIL 180
                ++L P+  Q S +N    N+NLNA +         ++E  N Y+  SG  E    
Sbjct: 121 -----IDLDPVFAQPSGTNQPVQNVNLNAEYI-------EIHEDINPYRGRSGFIEANGP 180

Query: 181 CSSASDPLLLPSGNSGLPVVANDGRAGSSLDGRRAPCKRKAIEGSVAQSSISGSSNYSQH 240
            +  S P        G     N    GSS++GRRA CKRKA+EGS++QSS  G  ++ Q 
Sbjct: 181 GTRVSQP--------GRSFEENGVGTGSSVEGRRASCKRKALEGSISQSSSGGYHDF-QR 240

Query: 241 IESGAETPLPVFPGRYNTGNRLTIPAPPARMNQASLRGAGELTSNSFSESIVAESSDSSQ 300
            ES + TP       +  GN L I        +  + G       S     V+  ++SS 
Sbjct: 241 GESSSWTPGSTV---FRPGNGLNISGSLDNGPRGMVSGTVPNFPVSAPNFPVSAIAESSS 300

Query: 301 RNYRIRISSSNAQDSFS----SAGTTVRHPGASSSQLPARLLPA-EHVLDLRPAPA-VDN 360
           RN  +R + S+ Q++ +    +AGT VR P   S    +R LPA +H LDLRP  + V +
Sbjct: 301 RNICVRSNPSDHQETVNPSTFAAGTVVRRPVPPSQLNLSRHLPADQHSLDLRPGQSFVVS 360

Query: 361 IPAQGQPIMIHVPALPRNLQPYRWNGSSTSRSGSSSSSAGERQVVQREEVRPSNMGRNVS 420
                 P+ I  P     L P+RW GSS    G+S+S+A   + +  +E R  ++  N  
Sbjct: 361 RNPNSTPVSI-PPGSRTMLPPFRWTGSSLV-GGTSNSTAPVERNLHLDETRSRSIPGNTL 420

Query: 421 EHPMFVPANELRNLVRNPSSRGITSSNLSIPGNVASTSRVGSSSGVHPSAGP-----WMP 480
           E PMF  A E+ N  R+ SSR +T+ NL+   + +S SR GS++ V P   P     W  
Sbjct: 421 EIPMFA-APEVGNFARSQSSRNVTNGNLN---SASSVSRTGSTTSVPPPPPPSSNLAWTS 480

Query: 481 PENSPTQFPRRL--TEYVRRQLFSS----ATNESGGRTSNYSQRSGSTSAQDMVLS-SGS 540
            +NSP    RR   +E  RR L SS    ATN+  G        +   S+  +VL   G 
Sbjct: 481 YQNSPHYQRRRTERSELARRSLLSSLAADATNQRSGDHPTLRSLAPPASSDGLVLQPGGD 540

Query: 541 GRRGHGLLQPRSALWMERRGDGGLGLPYSLRTLAASGEGSDNNRLVSEQIRNVLGLVRR- 600
             + H     R+    +R+GD  +G+P+ LR LAA+  G   +RL+  Q++NVL ++RR 
Sbjct: 541 NSQMHNRAYSRAGPLFDRQGDSVVGIPHPLRALAAASRG--RSRLMVSQMQNVLDVMRRD 600

Query: 601 --GESLRVEDVMILDQSLFFGMADIYDRHRDMRLDVDNMSYEELLALEERIGNVNTGLNE 660
               +LR+EDVM+L+ S+ F  A  +DR+RDMRLDVDNMSYEELLALEERIG+V TG+NE
Sbjct: 601 ANNNNLRLEDVMLLNHSVLFDGATGHDRYRDMRLDVDNMSYEELLALEERIGDVCTGVNE 660

Query: 661 ETIVARLKQKKHINATESQVEEEPCCVCQEEYIEGEDIGTLECGHDFHTHCIKQWLMQKN 708
           ETI  RLKQ+K+ + T+S  + EPCCVCQEEY EGED+GTLECGH+FH+ CIK+WL QKN
Sbjct: 661 ETISNRLKQRKYKSNTKSPQDAEPCCVCQEEYTEGEDMGTLECGHEFHSQCIKEWLKQKN 682

BLAST of Lag0004255 vs. ExPASy Swiss-Prot
Match: O49500 (E3 ubiquitin-protein ligase MBR2 OS=Arabidopsis thaliana OX=3702 GN=MBR2 PE=1 SV=1)

HSP 1 Score: 302.8 bits (774), Expect = 1.1e-80
Identity = 265/727 (36.45%), Postives = 360/727 (49.52%), Query Frame = 0

Query: 1   MQGQRSSVGSLSETINFEHGSSSNNPGNQPVYWNNMWNPAENRIPDYLLPTSDINTGYVS 60
           MQG RS+ G  S  IN+  G    +  N     NN+ NP + + P+    T      Y S
Sbjct: 1   MQGPRST-GDSSTGINYADGEPICST-NSETTSNNILNPVDVQFPNN--TTGSGRPTYAS 60

Query: 61  SVSHEQRSLSRWSLGEPSSCDMQTEALPDEQKAELGWSSVTRDADGPVTDNRLCEPSNNP 120
           S SH  ++ + WS GE SS               LG  S   +++G  TD +L       
Sbjct: 61  SSSHVVQNHNWWSFGESSS--------------RLG-PSDHLNSNGSKTDRQLLSDGYGF 120

Query: 121 SLGHVNLSPLIIQNSNSNAIPHNLNLNASFASHGGDNSRVNEGSNVYKSSGSEEGRILCS 180
             G   +            +P    L  S +SH    S VN G ++   SG +   ++  
Sbjct: 121 EEGQSGM-----------LLPGESFLRGSSSSH--MLSHVNLGKDMDIGSGLQTSGVVIR 180

Query: 181 SASDPLLLPSGNSGLPVVANDGRAGSSLDGRRAPCKRKAIEGSVAQSSISGSSNYSQHIE 240
             +    L  G+S           GSSL G  + CKRKA+EG+ + S    S       E
Sbjct: 181 HNNCETSL--GSSSQTAEERSSGPGSSLGGLGSSCKRKALEGAPSHSFPGESHGCFFQTE 240

Query: 241 SGAETPLPVFPGRYNTGNRLTIPAP----PARMNQA-------SLRGAGELTSNSFSESI 300
           +GA         +Y+  + L++  P    P   NQ+        L G   +T+++F  + 
Sbjct: 241 NGAWNE---GLAQYDASSSLSLSMPSQNSPNVNNQSGLPEPRFGLGGGRAVTASAFPSTR 300

Query: 301 VAESSDSSQRNYRIRISSSNAQDSFSSAGTTVRHPGASSSQLPARLLPAEHVLDLRPAPA 360
             E+     R         +   SF+ +G++VR       QLPA   P    LD R  P 
Sbjct: 301 STETISRPGRRLNPGQPPESVAFSFTQSGSSVR----QQQQLPA-TSPFVDPLDARAIPV 360

Query: 361 V-DNIPAQGQPIMIHVPALPRNLQPYRWNGSSTSRSGSSSSSAGERQVVQREEVRPSNMG 420
              +    GQP MIH+PAL RN+  + W+ SS+SR+ S          +  E + P +  
Sbjct: 361 TGSSSSGDGQPSMIHLPALTRNIHQFAWSASSSSRANS----------MPEEGLSPWDAP 420

Query: 421 RNVSEHPMF-VPANELRNLVRNPSSRGITSSNLSIPGNVASTSRVGSSSGVH--PSAGPW 480
           R  SE P+F  PANE RN V++      T  N S  G+     R GSSSG+H       W
Sbjct: 421 RINSEQPVFTTPANETRNPVQDQFCWSFTRGNPSTSGDSPFVPRAGSSSGIHGLQPNPTW 480

Query: 481 MPPENSPTQFPRRLTEYVRRQLFSSATNESG--GRTSNYSQRSGSTSAQDMVLSSGSGRR 540
           + P N       R++E     LF S  +ES   G +        S S+ +    SGS  R
Sbjct: 481 VTPHNQ-----SRISEVAPWSLFPSIESESATHGASLPLLPTGPSVSSNEAAAPSGSSSR 540

Query: 541 GHGLLQPRSALWMERRGDGGLGLPYSLRTLAASGEGSDNNRLVSEQIRNVLGLVRRGESL 600
            H   Q RS L +ER+ D  L L +  R+LAA  +G   NRL+SE IR VL  +RRGE+L
Sbjct: 541 SHRSRQRRSGLLLERQND-HLHLRHLGRSLAADNDG--RNRLISE-IRQVLSAMRRGENL 600

Query: 601 RVEDVMILDQSLFFGMADIYDRHRDMRLDVDNMSYEELLALEERIGNVNTGLNEETIVAR 660
           R ED M+ D  ++ GMA+++DRHRDMRLDVDNMSYEELLAL ERIG+V+TGL+EE I+  
Sbjct: 601 RFEDYMVFDPLIYQGMAEMHDRHRDMRLDVDNMSYEELLALGERIGDVSTGLSEEVILKV 660

Query: 661 LKQKKHI-NATESQVEEEPCCVCQEEYIEGEDIGTLECGHDFHTHCIKQWLMQKNLCPIC 710
           +KQ KH  +A  S  + EPCCVCQEEY EG+D+GTL CGH+FHT C+KQWLM KNLCPIC
Sbjct: 661 MKQHKHTSSAAGSHQDMEPCCVCQEEYAEGDDLGTLGCGHEFHTACVKQWLMLKNLCPIC 666

BLAST of Lag0004255 vs. ExPASy Swiss-Prot
Match: Q7XTV7 (Probable E3 ubiquitin-protein ligase HIP1 OS=Oryza sativa subsp. japonica OX=39947 GN=HIP1 PE=1 SV=2)

HSP 1 Score: 298.9 bits (764), Expect = 1.5e-79
Identity = 259/741 (34.95%), Postives = 377/741 (50.88%), Query Frame = 0

Query: 1   MQGQRSSVGSLSETINFEHGSSSNNP-GNQPVYWNNMWNPAE-NRIPDYLLPTSDINTGY 60
           MQGQ++SV  L++   F+H SSS NP  +Q  YWNN+    E + +  Y +  SD    Y
Sbjct: 1   MQGQKNSVEQLADVFGFDHASSSGNPVMDQQGYWNNILGSVESHNLQGYQVNRSDGTIPY 60

Query: 61  VSSVSHEQRSLSRWSLGEPSSCDMQTE-ALPDEQKAELGWSSVTRDADG--PVTDNRLCE 120
            + V      L  W  GE S+          +E KAE       R+  G   + + RL  
Sbjct: 61  GNGVHQNGTFLGFWESGEASASGSSLHFGGSNEIKAE------QRNIGGGLRIGERRLVA 120

Query: 121 PSN----NPSLG-HVNLSPLIIQNSNSNAIPHNLNLNASFASHGGDNSRVNE------GS 180
             N    N  +G ++N + L  +NSN N       L+    SH G N + +E       +
Sbjct: 121 ERNLSLDNVDIGLNINGNDLSGENSNVNGASQGSELHGG-CSHTGSNGQASELRLHPYRT 180

Query: 181 NVYKSSGSEEGRILCSSASDPLLLPSGNSGLPVVANDGRAGSSLDGRRAPCKRKAIEGSV 240
            +  +   E    L  S +     P G+  L     D R GSSLDGRR  CKRK IEG  
Sbjct: 181 FILGADQPEPFNSLNGSEN-----PLGDFSLMPEGIDQRPGSSLDGRRLACKRKNIEGVN 240

Query: 241 AQSSISGSSNYSQHIESGAETPLPVFPGRYNTGNRLTIPAP-----PARMNQASLR-GA- 300
            Q S   S+++S   +S       +    +N      +P+P     P+ +++   R GA 
Sbjct: 241 GQCSAGASTSFSHRNDSIFHN---IASSSHNPSPSTNLPSPNCLLVPSTLDEQLPRYGAT 300

Query: 301 -GELTSNSFSESIVAESSDSSQRNYRIRISSSNAQDSFS--SAGTTVRHPGASSSQLPAR 360
              L+S+S+  S    +S  SQR++R R S +     +    + +T+RH  + + Q P  
Sbjct: 301 TAGLSSSSYDPSGGNNNSGGSQRSFRPRTSLAQHIGPYGVWPSSSTIRHSNSWNHQPP-- 360

Query: 361 LLPAEHVLDLRP--APAVDNIPAQGQPIMIHVPALPRNLQPYRWNGSSTSRSGSSSSSAG 420
             P +   D  P   P V ++  Q Q  M  VP +P+    +   G+S+SR+G+      
Sbjct: 361 --PFQSSFDEPPEVIPVVSSLNFQYQHPMNVVPGIPQMSHRFTGPGASSSRTGNL----- 420

Query: 421 ERQVVQREEVRPSNMGRNVSEHPMFVP--ANELRNLVRNPSSRGITSSNLSIPGNVASTS 480
           E +++  EE    N+    +  P  VP  A ++R+L+  PSS  +     +IPGNV S+S
Sbjct: 421 ENRIIGSEEFSARNV--VATSFPDAVPPAALDMRHLIPEPSSWNVDGRATTIPGNVPSSS 480

Query: 481 RVGSSSGVHPSAG-PWMPPENSPTQFPRRLTEYVRRQLFSSATNESGGRTSNYSQRSGST 540
           R  ++S V+P AG P++  +N   + PR L+E +                   S+ SG+ 
Sbjct: 481 RANTNSMVNPPAGSPFIAHQNLHRRNPRNLSEEI-------------------SRLSGAL 540

Query: 541 SAQDMVLSSGSGRRGHGLLQPRSALWMERRGDGGLGLPYSLRTLAASGEGSDNNRLVSEQ 600
                        RGH   + RS   +ER+GDG  G+P S R+        +  RL+  +
Sbjct: 541 -------------RGHQHPRLRSGFLLERQGDGVWGVPLSTRS-------REGRRLI--E 600

Query: 601 IRNVLGLVRRGESLRVEDVMILDQSLFFGMADIYDRHRDMRLDVDNMSYEELLALEERIG 660
           IRN L ++ RGE++R E       S+F+G  DI+DRHRDMRLD+DNMSYEELLALEERIG
Sbjct: 601 IRNALEMIHRGENVRFE-------SIFYGGVDIHDRHRDMRLDIDNMSYEELLALEERIG 660

Query: 661 NVNTGLNEETIVARLKQKKHIN-ATESQVEEEPCCVCQEEYIEGEDIGTLECGHDFHTHC 710
           NV+TGL+EE +   LKQ+K  +   E+ VEEEPCC+CQEEY++G+D+GTL+CGHDFH  C
Sbjct: 661 NVSTGLSEEEVTKLLKQRKFSSWRLEASVEEEPCCICQEEYVDGDDLGTLDCGHDFHVGC 667

BLAST of Lag0004255 vs. ExPASy Swiss-Prot
Match: Q9ZQF9 (E3 ubiquitin-protein ligase MBR1 OS=Arabidopsis thaliana OX=3702 GN=MBR1 PE=1 SV=1)

HSP 1 Score: 275.0 bits (702), Expect = 2.4e-72
Identity = 247/754 (32.76%), Postives = 360/754 (47.75%), Query Frame = 0

Query: 1   MQGQRSSVGSLSETINFEHGSSSNNPGNQPVY-----WNNMWNPAENRIPDYLLPTSDIN 60
           MQG RS  GS +E          N    + +Y      N M NPA+   P+   P+    
Sbjct: 4   MQGPRSIGGSSTEV---------NQVDGESIYCTETSLNTMLNPADTGFPNNSTPSG--R 63

Query: 61  TGYVSSVSHEQRSLSRWSLGEPSSCDMQTEAL------------PDEQKAELGWSSVTRD 120
             Y SS SH  +  + W  GE SS    ++ +             D     +G+ S  R 
Sbjct: 64  PTYASSSSHAAQDHTWWRFGESSSIPGPSDQVNSIGIKTSHQLPQDGTHHFVGYGSEGRQ 123

Query: 121 A--DGPVTDNRLCEPSNNPSLGHVNLSPLIIQNSNSNAIPHNLNLNASFASH--GGDNSR 180
              +G + D  +   S      H+   P  ++ S+SN +P +++++    S       S 
Sbjct: 124 TGLNGMMVDGGVHAGS------HIRNVPSFLRGSSSNPMPQHVDMSMDMDSDNCNAQTSG 183

Query: 181 VNEGSNVYKSSGSEEGRILCSSASDPLLLPSGNSGLPVVANDGRAGSSLDGRRAPCKRKA 240
           V    N Y SS     +    S+S P                    S   G  + CKRKA
Sbjct: 184 VVIRHNSYGSSLGSSVQAAGESSSGP-------------------ASPFGGWGSSCKRKA 243

Query: 241 IEGSVAQSSISGSSNYSQHIESGAETPLPVFPGRYNTGNRLTIPAPP----------ARM 300
           +EGS +      + N     E+ A         +Y   + L++  P            R 
Sbjct: 244 LEGSPSHYFSGETPNRIVQTENSAS---HASLSQYGASSSLSLATPSQSSPNVTNHFGRT 303

Query: 301 NQASLRGAGE-LTSNSFSESIVAESSDSSQRNYRIRISSSNAQDSFSSAGTTVRHPGASS 360
            Q    G G  + +++F  +   ++   + R    R    +   S S  GT+VR  G+  
Sbjct: 304 EQMFGSGGGRAVAASAFHSTRNTDTLSRAGRRLNPRQPQESVAFSVSHGGTSVRPTGSLQ 363

Query: 361 SQLPARLLPAEHVLDLRPAPAVD-NIPAQGQPIMIHVPALPRNLQPYRWNGSSTSRSGSS 420
             LP    P     D+R +     +   + Q  ++H+PAL RN+  Y W+ S +SR+ S+
Sbjct: 364 QNLPLN-SPFVDPPDVRSSSITSGSNTGENQTNIVHLPALTRNIHQYAWDASFSSRA-SN 423

Query: 421 SSSAGERQVVQREEVRPS-NMGRNVSEHPMFVPANELRNLVRN-----PSSRGITSSNLS 480
            S  G    +  E + P     R+  E P+F PA ++R  V +       S G +  +L 
Sbjct: 424 PSGIG----MPAERLGPQWETPRSNQEQPLFAPATDMRQPVHDLWNFARGSPGSSVDSLF 483

Query: 481 IPGNVASTSRVGSSSGVH-PSAGP-WMPPENSPTQFPRRLTEYVRRQLFSS--ATNESGG 540
           +P       R G SS +H P   P W+PP+N+P   P R +E     LF S  + + S G
Sbjct: 484 VP-------RAGPSSAIHTPQPNPTWIPPQNAPPHNPSRTSELSPWSLFPSIESPSASHG 543

Query: 541 RTSNYSQRSGSTSAQDMVLSSGSGRRGHGLLQPRSALWMERRGDGGLGLPYSLRTLAASG 600
                     S S+ ++ + S S  R H     RS L +ER+ +  L L +  R+LAA G
Sbjct: 544 GPLPLLPAGPSVSSNEVTMPSSSNSRSHRSRHRRSGLLLERQNE-LLHLRHIGRSLAADG 603

Query: 601 EGSDNNRLVSEQIRNVLGLVRRGESLRVEDVMILDQSLFFGMADIYDRHRDMRLDVDNMS 660
            G   N+++SE IR VL  +RRGE+LRVED M+ D  ++ GM D++DRHR+MRLDVDNMS
Sbjct: 604 NG--RNQIISE-IRQVLHAMRRGENLRVEDYMVFDPLIYQGMTDMHDRHREMRLDVDNMS 663

Query: 661 YEELLALEERIGNVNTGLNEETIVARLKQKKHINATESQVE----EEPCCVCQEEYIEGE 708
           YEELLAL ERIG+V+TGL+EE I+  +KQ KH +++ S VE     EPCC+CQEEY+EG+
Sbjct: 664 YEELLALGERIGDVSTGLSEEVILKAMKQHKHTSSSPSSVELHQNIEPCCICQEEYVEGD 701

BLAST of Lag0004255 vs. ExPASy Swiss-Prot
Match: Q5QLR5 (Probable E3 ubiquitin-protein ligase ZFP1 OS=Oryza sativa subsp. japonica OX=39947 GN=ZFP1 PE=1 SV=1)

HSP 1 Score: 145.6 bits (366), Expect = 2.2e-33
Identity = 70/132 (53.03%), Postives = 93/132 (70.45%), Query Frame = 0

Query: 586 EDVMILDQSLFFGMADIYDRHRDMRLDVDNMSYEELLALEERIGNVNTGLNEETIVARLK 645
           E   I+D S  + ++++ D HRDMRLD+D+M+YEELLALEE+IG+VNTGL +  IV +LK
Sbjct: 387 EHATIMDFSRLYEVSNVVDEHRDMRLDIDSMTYEELLALEEQIGDVNTGLAKSYIVEKLK 446

Query: 646 QKKHI--------NATESQVEEEPCCVCQEEYIEGEDIGTLECGHDFHTHCIKQWLMQKN 705
               +         ++ES +E + C +CQEEY   E IGTL+CGH +H  CIKQWLM KN
Sbjct: 447 TSLFVPGSSCMSNKSSESSMENDACIICQEEYQVKECIGTLDCGHRYHEDCIKQWLMVKN 506

Query: 706 LCPICKTTGLAS 710
           LCPICKTT L++
Sbjct: 507 LCPICKTTALST 518

BLAST of Lag0004255 vs. ExPASy TrEMBL
Match: A0A1S4DVI9 (probable E3 ubiquitin-protein ligase HIP1 OS=Cucumis melo OX=3656 GN=LOC103487873 PE=4 SV=1)

HSP 1 Score: 1286.6 bits (3328), Expect = 0.0e+00
Identity = 652/713 (91.44%), Postives = 687/713 (96.35%), Query Frame = 0

Query: 1   MQGQRSSVGSLSETINFEHGSSSNNPGNQPVYWNNMWNPAENRIPDYLLPTSDINTGYVS 60
           MQGQRSSVGSLSETINFEHGSSSNNPGNQPVYWNN+WNPAENRIPDYLLPTSD+N+GYV+
Sbjct: 1   MQGQRSSVGSLSETINFEHGSSSNNPGNQPVYWNNVWNPAENRIPDYLLPTSDVNSGYVN 60

Query: 61  SVSHEQRSLSRWSLGEPSSCDMQTEALPDEQKAELGWSSVTRDADGPVTDNRLCEPSNNP 120
           SVSHEQRSLSRWSLGEPSSCDMQTE LPDEQKAE+GWSS+  DADGPVT+NRLCEPSNNP
Sbjct: 61  SVSHEQRSLSRWSLGEPSSCDMQTEMLPDEQKAEIGWSSMASDADGPVTENRLCEPSNNP 120

Query: 121 SLGHVNLSPLIIQ--NSNSNAIPHNLNLNASFASHGGDNSRVNEGSNVYKSSGSEEGRIL 180
           SLGHVNLSPLIIQ  NSNSNAIPHNLNLN+SF SHGGDNSRVNEG+NVYKSSG EEGRIL
Sbjct: 121 SLGHVNLSPLIIQNSNSNSNAIPHNLNLNSSFVSHGGDNSRVNEGTNVYKSSGPEEGRIL 180

Query: 181 CSSASDPLLLPSGNSGLPVVANDGRAGSSLDGRRAPCKRKAIEGSVAQSSISGSSNYSQH 240
           CS+ASDPLLLPSGNSGLPVV NDGR GSSLDGRRAPCKRK+IEG+VAQSS+SGSSNYSQH
Sbjct: 181 CSNASDPLLLPSGNSGLPVVGNDGRPGSSLDGRRAPCKRKSIEGNVAQSSLSGSSNYSQH 240

Query: 241 IESGAETPLPVFPGRYNTGNRLTIPAPPARMNQASLRGAGELTSNSFSESIVAESSDSSQ 300
           IESG+E  LPVFPGRYNTG+R++IP PPARMNQA +RGAGELTSN+FSESIVAESSDSSQ
Sbjct: 241 IESGSEAHLPVFPGRYNTGSRISIPPPPARMNQAPVRGAGELTSNNFSESIVAESSDSSQ 300

Query: 301 RNYRIRISSSNAQDSFSSAGTTVRHPGASSSQLPARLLPAEHVLDLRPAPAVDNIPAQGQ 360
           RNYRIRISSSNAQDSF+SAGT VRHPG  SSQLPARLLPAEH+LDLRPAPAVDN+ AQGQ
Sbjct: 301 RNYRIRISSSNAQDSFASAGTAVRHPGPPSSQLPARLLPAEHILDLRPAPAVDNVSAQGQ 360

Query: 361 PIMIHVPALPRNLQPYRWNGSSTSRSGSSSSSAGERQVVQREEVRPSNMGRNVSEHPMFV 420
           PIMIHVPALPRNLQPYRWNGSSTSRSGSSSSSAGERQVVQREEVRPS MGRNVSEHPMF+
Sbjct: 361 PIMIHVPALPRNLQPYRWNGSSTSRSGSSSSSAGERQVVQREEVRPSIMGRNVSEHPMFL 420

Query: 421 PANELRNLVRNPSSRGITSSNLSIPGNVASTSRVGSSSGVHPSAGPWMPPENSPTQFPRR 480
           PANELR LVR+PSSR + SSNL+IPGNVASTSRVGSSSGVHPS+GPW+PPE+SPTQFPRR
Sbjct: 421 PANELRGLVRSPSSRALASSNLAIPGNVASTSRVGSSSGVHPSSGPWIPPESSPTQFPRR 480

Query: 481 LTEYVRRQLFSSATNESGGRTSNYSQRSGSTSAQDMVLSSGSGRRGHGLLQPRSALWMER 540
           LTEYVRRQLF+SATNESGGRTSNYSQRSGSTSAQ+MVLSSGSGRRGH LLQPRSALWMER
Sbjct: 481 LTEYVRRQLFASATNESGGRTSNYSQRSGSTSAQEMVLSSGSGRRGHHLLQPRSALWMER 540

Query: 541 RGDGGLGLPYSLRTLAASGEGSDNNRLVSEQIRNVLGLVRRGESLRVEDVMILDQSLFFG 600
           RGDGGLGLPYSLRTLA+S EGSDNNRLVSE +RNVLGLVRRGESLRVEDVMILDQSLFFG
Sbjct: 541 RGDGGLGLPYSLRTLASSAEGSDNNRLVSEHLRNVLGLVRRGESLRVEDVMILDQSLFFG 600

Query: 601 MADIYDRHRDMRLDVDNMSYEELLALEERIGNVNTGLNEETIVARLKQKKHINATESQVE 660
           MADIYDRHRDMRLDVDNMSYEELLALEERIGNVNTGLNEETIVARLKQKKH+NA ESQVE
Sbjct: 601 MADIYDRHRDMRLDVDNMSYEELLALEERIGNVNTGLNEETIVARLKQKKHVNAVESQVE 660

Query: 661 EEPCCVCQEEYIEGEDIGTLECGHDFHTHCIKQWLMQKNLCPICKTTGLASRE 712
           EEPCCVCQEEY+EGEDIGTLECGHDFHT CIKQWLMQKNLCPICKTTGLASRE
Sbjct: 661 EEPCCVCQEEYVEGEDIGTLECGHDFHTACIKQWLMQKNLCPICKTTGLASRE 713

BLAST of Lag0004255 vs. ExPASy TrEMBL
Match: E5GB80 (Zinc finger protein binding protein OS=Cucumis melo subsp. melo OX=412675 PE=4 SV=1)

HSP 1 Score: 1286.6 bits (3328), Expect = 0.0e+00
Identity = 652/713 (91.44%), Postives = 687/713 (96.35%), Query Frame = 0

Query: 1   MQGQRSSVGSLSETINFEHGSSSNNPGNQPVYWNNMWNPAENRIPDYLLPTSDINTGYVS 60
           MQGQRSSVGSLSETINFEHGSSSNNPGNQPVYWNN+WNPAENRIPDYLLPTSD+N+GYV+
Sbjct: 1   MQGQRSSVGSLSETINFEHGSSSNNPGNQPVYWNNVWNPAENRIPDYLLPTSDVNSGYVN 60

Query: 61  SVSHEQRSLSRWSLGEPSSCDMQTEALPDEQKAELGWSSVTRDADGPVTDNRLCEPSNNP 120
           SVSHEQRSLSRWSLGEPSSCDMQTE LPDEQKAE+GWSS+  DADGPVT+NRLCEPSNNP
Sbjct: 61  SVSHEQRSLSRWSLGEPSSCDMQTEMLPDEQKAEIGWSSMASDADGPVTENRLCEPSNNP 120

Query: 121 SLGHVNLSPLIIQ--NSNSNAIPHNLNLNASFASHGGDNSRVNEGSNVYKSSGSEEGRIL 180
           SLGHVNLSPLIIQ  NSNSNAIPHNLNLN+SF SHGGDNSRVNEG+NVYKSSG EEGRIL
Sbjct: 121 SLGHVNLSPLIIQNSNSNSNAIPHNLNLNSSFVSHGGDNSRVNEGTNVYKSSGPEEGRIL 180

Query: 181 CSSASDPLLLPSGNSGLPVVANDGRAGSSLDGRRAPCKRKAIEGSVAQSSISGSSNYSQH 240
           CS+ASDPLLLPSGNSGLPVV NDGR GSSLDGRRAPCKRK+IEG+VAQSS+SGSSNYSQH
Sbjct: 181 CSNASDPLLLPSGNSGLPVVGNDGRPGSSLDGRRAPCKRKSIEGNVAQSSLSGSSNYSQH 240

Query: 241 IESGAETPLPVFPGRYNTGNRLTIPAPPARMNQASLRGAGELTSNSFSESIVAESSDSSQ 300
           IESG+E  LPVFPGRYNTG+R++IP PPARMNQA +RGAGELTSN+FSESIVAESSDSSQ
Sbjct: 241 IESGSEAHLPVFPGRYNTGSRISIPPPPARMNQAPVRGAGELTSNNFSESIVAESSDSSQ 300

Query: 301 RNYRIRISSSNAQDSFSSAGTTVRHPGASSSQLPARLLPAEHVLDLRPAPAVDNIPAQGQ 360
           RNYRIRISSSNAQDSF+SAGT VRHPG  SSQLPARLLPAEH+LDLRPAPAVDN+ AQGQ
Sbjct: 301 RNYRIRISSSNAQDSFASAGTAVRHPGPPSSQLPARLLPAEHILDLRPAPAVDNVSAQGQ 360

Query: 361 PIMIHVPALPRNLQPYRWNGSSTSRSGSSSSSAGERQVVQREEVRPSNMGRNVSEHPMFV 420
           PIMIHVPALPRNLQPYRWNGSSTSRSGSSSSSAGERQVVQREEVRPS MGRNVSEHPMF+
Sbjct: 361 PIMIHVPALPRNLQPYRWNGSSTSRSGSSSSSAGERQVVQREEVRPSIMGRNVSEHPMFL 420

Query: 421 PANELRNLVRNPSSRGITSSNLSIPGNVASTSRVGSSSGVHPSAGPWMPPENSPTQFPRR 480
           PANELR LVR+PSSR + SSNL+IPGNVASTSRVGSSSGVHPS+GPW+PPE+SPTQFPRR
Sbjct: 421 PANELRGLVRSPSSRALASSNLAIPGNVASTSRVGSSSGVHPSSGPWIPPESSPTQFPRR 480

Query: 481 LTEYVRRQLFSSATNESGGRTSNYSQRSGSTSAQDMVLSSGSGRRGHGLLQPRSALWMER 540
           LTEYVRRQLF+SATNESGGRTSNYSQRSGSTSAQ+MVLSSGSGRRGH LLQPRSALWMER
Sbjct: 481 LTEYVRRQLFASATNESGGRTSNYSQRSGSTSAQEMVLSSGSGRRGHHLLQPRSALWMER 540

Query: 541 RGDGGLGLPYSLRTLAASGEGSDNNRLVSEQIRNVLGLVRRGESLRVEDVMILDQSLFFG 600
           RGDGGLGLPYSLRTLA+S EGSDNNRLVSE +RNVLGLVRRGESLRVEDVMILDQSLFFG
Sbjct: 541 RGDGGLGLPYSLRTLASSAEGSDNNRLVSEHLRNVLGLVRRGESLRVEDVMILDQSLFFG 600

Query: 601 MADIYDRHRDMRLDVDNMSYEELLALEERIGNVNTGLNEETIVARLKQKKHINATESQVE 660
           MADIYDRHRDMRLDVDNMSYEELLALEERIGNVNTGLNEETIVARLKQKKH+NA ESQVE
Sbjct: 601 MADIYDRHRDMRLDVDNMSYEELLALEERIGNVNTGLNEETIVARLKQKKHVNAVESQVE 660

Query: 661 EEPCCVCQEEYIEGEDIGTLECGHDFHTHCIKQWLMQKNLCPICKTTGLASRE 712
           EEPCCVCQEEY+EGEDIGTLECGHDFHT CIKQWLMQKNLCPICKTTGLASRE
Sbjct: 661 EEPCCVCQEEYVEGEDIGTLECGHDFHTACIKQWLMQKNLCPICKTTGLASRE 713

BLAST of Lag0004255 vs. ExPASy TrEMBL
Match: A0A6J1GIQ0 (probable E3 ubiquitin-protein ligase RHG1A isoform X1 OS=Cucurbita moschata OX=3662 GN=LOC111454242 PE=4 SV=1)

HSP 1 Score: 1284.2 bits (3322), Expect = 0.0e+00
Identity = 653/711 (91.84%), Postives = 681/711 (95.78%), Query Frame = 0

Query: 1   MQGQRSSVGSLSETINFEHGSSSNNPGNQPVYWNNMWNPAENRIPDYLLPTSDINTGYVS 60
           MQGQRSSVGSLSETINFEHGSSSNNPG+QPVYWNNMWNPAENRIPDYLLPT+DINTGYVS
Sbjct: 1   MQGQRSSVGSLSETINFEHGSSSNNPGSQPVYWNNMWNPAENRIPDYLLPTTDINTGYVS 60

Query: 61  SVSHEQRSLSRWSLGEPSSCDMQTEALPDEQKAELGWSSVTRDADGPVTDNRLCEPSNNP 120
           SVSHEQR+LSRWSLGEPSSCDMQTEAL DEQK E+GWSSV RDADGPVT+ RLCEPSNNP
Sbjct: 61  SVSHEQRNLSRWSLGEPSSCDMQTEALSDEQKGEVGWSSVARDADGPVTETRLCEPSNNP 120

Query: 121 SLGHVNLSPLIIQNSNSNAIPHNLNLNASFASHGGDNSRVNEGSNVYKSSGSEEGRILCS 180
           +LGHVN SPLIIQNSNSNA+PH+LNLNASFASHGG++SRVNEGSN+YKSSGSEEGR LCS
Sbjct: 121 TLGHVNFSPLIIQNSNSNALPHSLNLNASFASHGGESSRVNEGSNLYKSSGSEEGRALCS 180

Query: 181 SASDPLLLPSGNSGLPVVANDGRAGSSLDGRRAPCKRKAIEGSVAQSSISGSSNYSQHIE 240
           SASDPLLLPSGNSGLPVVANDGRAGSSLDGRRAPCKRKAIEGSV Q S+SGSSNY+QHIE
Sbjct: 181 SASDPLLLPSGNSGLPVVANDGRAGSSLDGRRAPCKRKAIEGSVTQPSLSGSSNYAQHIE 240

Query: 241 SGAETPLPVFPGRYNTGNRLTIPAPPARMNQASLRGAGELTSNSFSESIVAESSDSSQRN 300
           SGAE  LPVFPGRYNTG+RLTIPAPPARMNQA +RGAGE+TSNSFSES+VAESSDSSQRN
Sbjct: 241 SGAEPTLPVFPGRYNTGSRLTIPAPPARMNQAPVRGAGEMTSNSFSESMVAESSDSSQRN 300

Query: 301 YRIRISSSNAQDSFSSAGTTVRHPGASSSQLPARLLPAEHVLDLRPAPAVDNIPAQGQPI 360
           YRIRISSSNAQDSF+  G TVRHPGASSSQLPAR+LPAEHVLDLRPAPAVDNI AQGQP+
Sbjct: 301 YRIRISSSNAQDSFAPTGMTVRHPGASSSQLPARILPAEHVLDLRPAPAVDNISAQGQPL 360

Query: 361 MIHVPALPRNLQPYRWNGSSTSRSGSSSSSAGERQVVQREEVRPSNMGRNVSEHPMFVPA 420
           MIHVPALPR+LQPYRWNGSSTSRSG SSSSAGERQVVQREEVRPSNMGRNVSEHPMFVPA
Sbjct: 361 MIHVPALPRSLQPYRWNGSSTSRSGGSSSSAGERQVVQREEVRPSNMGRNVSEHPMFVPA 420

Query: 421 NELRNLVRNPSSRGITSSNLSIPGNVASTSRVGSSSGVHPSAGPWMPPENSPTQFPRRLT 480
           NELRNLVRNPSSRG+ SSNLSIPG VASTSRVGSSSGVHP +GPWMPPENSPTQFPRRLT
Sbjct: 421 NELRNLVRNPSSRGLASSNLSIPGTVASTSRVGSSSGVHPPSGPWMPPENSPTQFPRRLT 480

Query: 481 EYVRRQLFSSATNESGGRTSNYSQRSGSTSAQDMVLSSGSGRRGHGLLQPRSALWMERRG 540
           EYVRRQLFSSATNESGGRTSNYSQRSGSTSAQDMVLSSGSGRRGH LLQPRSALWMERRG
Sbjct: 481 EYVRRQLFSSATNESGGRTSNYSQRSGSTSAQDMVLSSGSGRRGHHLLQPRSALWMERRG 540

Query: 541 DGGLGLPYSLRTLAASGEGSDNNRLVSEQIRNVLGLVRRGESLRVEDVMILDQSLFFGMA 600
           D GLGLPYSLRTLA SGEGSDNNRLVSEQIRNVLGLVRRGESLRVEDVMILDQSLFFGMA
Sbjct: 541 DTGLGLPYSLRTLATSGEGSDNNRLVSEQIRNVLGLVRRGESLRVEDVMILDQSLFFGMA 600

Query: 601 DIYDRHRDMRLDVDNMSYEELLALEERIGNVNTGLNEETIVARLKQKKHINATESQVEEE 660
           DIYDRHRDMRLDVDNMSYEELLALEERIGNVNTGL+EETIVARLK+ KH+NA E QVEEE
Sbjct: 601 DIYDRHRDMRLDVDNMSYEELLALEERIGNVNTGLSEETIVARLKRTKHVNAPEPQVEEE 660

Query: 661 PCCVCQEEYIEGEDIGTLECGHDFHTHCIKQWLMQKNLCPICKTTGLASRE 712
           PCCVCQEEY EGEDIG L+CGH FHT CIKQWLMQKN+CPICKTTG ASRE
Sbjct: 661 PCCVCQEEYKEGEDIGMLDCGHKFHTQCIKQWLMQKNICPICKTTGFASRE 711

BLAST of Lag0004255 vs. ExPASy TrEMBL
Match: A0A6J1KSD9 (probable E3 ubiquitin-protein ligase RHG1A isoform X1 OS=Cucurbita maxima OX=3661 GN=LOC111496039 PE=4 SV=1)

HSP 1 Score: 1282.7 bits (3318), Expect = 0.0e+00
Identity = 650/711 (91.42%), Postives = 683/711 (96.06%), Query Frame = 0

Query: 1   MQGQRSSVGSLSETINFEHGSSSNNPGNQPVYWNNMWNPAENRIPDYLLPTSDINTGYVS 60
           MQGQRSSVGSLSETINFEHGSSSNNPG+QPVYWNNMWNPAENRIPDYLLPT+DINTGYV+
Sbjct: 1   MQGQRSSVGSLSETINFEHGSSSNNPGSQPVYWNNMWNPAENRIPDYLLPTTDINTGYVT 60

Query: 61  SVSHEQRSLSRWSLGEPSSCDMQTEALPDEQKAELGWSSVTRDADGPVTDNRLCEPSNNP 120
           +VSHEQR+LSRWSLGEPSSCDMQTEAL DEQK E+GWSSV+RDADGPVT+ RLCEPSNNP
Sbjct: 61  TVSHEQRNLSRWSLGEPSSCDMQTEALSDEQKGEVGWSSVSRDADGPVTETRLCEPSNNP 120

Query: 121 SLGHVNLSPLIIQNSNSNAIPHNLNLNASFASHGGDNSRVNEGSNVYKSSGSEEGRILCS 180
           +LGHVN SP+IIQNSNSNA+PH+LNLNASFASHGG++SRVNEGSN+YKS+GSEEGR LCS
Sbjct: 121 TLGHVNFSPMIIQNSNSNALPHSLNLNASFASHGGESSRVNEGSNLYKSNGSEEGRALCS 180

Query: 181 SASDPLLLPSGNSGLPVVANDGRAGSSLDGRRAPCKRKAIEGSVAQSSISGSSNYSQHIE 240
           SASDPLLLPSGNSGLPVVANDGRAGSSLDGRRAPCKRKAIEGSV Q S+SGSSNY+QHIE
Sbjct: 181 SASDPLLLPSGNSGLPVVANDGRAGSSLDGRRAPCKRKAIEGSVTQPSLSGSSNYAQHIE 240

Query: 241 SGAETPLPVFPGRYNTGNRLTIPAPPARMNQASLRGAGELTSNSFSESIVAESSDSSQRN 300
           SGAE  LPVFPGRYNTG+RLTIPAPPARMNQA +RGAGE+TSNSFSES+VAESSDSSQRN
Sbjct: 241 SGAEPTLPVFPGRYNTGSRLTIPAPPARMNQAPVRGAGEMTSNSFSESMVAESSDSSQRN 300

Query: 301 YRIRISSSNAQDSFSSAGTTVRHPGASSSQLPARLLPAEHVLDLRPAPAVDNIPAQGQPI 360
           YRIRISSSNAQDSF+  G TVRHPGASSSQLPAR+LPAEHVLDLRPAPAVDNI AQGQP+
Sbjct: 301 YRIRISSSNAQDSFAPTGMTVRHPGASSSQLPARILPAEHVLDLRPAPAVDNISAQGQPL 360

Query: 361 MIHVPALPRNLQPYRWNGSSTSRSGSSSSSAGERQVVQREEVRPSNMGRNVSEHPMFVPA 420
           MIHVPALPR+LQPYRWNGSSTSRSGSSSSSAGERQVVQREEVRPSNMGRNVSEHPMFVPA
Sbjct: 361 MIHVPALPRSLQPYRWNGSSTSRSGSSSSSAGERQVVQREEVRPSNMGRNVSEHPMFVPA 420

Query: 421 NELRNLVRNPSSRGITSSNLSIPGNVASTSRVGSSSGVHPSAGPWMPPENSPTQFPRRLT 480
           NELRNLVRNPSSRG+ SSNLSIPG VASTSRVGSSSGVHP +GPWMPPENSPTQFPRRLT
Sbjct: 421 NELRNLVRNPSSRGLASSNLSIPGTVASTSRVGSSSGVHPPSGPWMPPENSPTQFPRRLT 480

Query: 481 EYVRRQLFSSATNESGGRTSNYSQRSGSTSAQDMVLSSGSGRRGHGLLQPRSALWMERRG 540
           EYVRRQLFSSATNESGGRTSNYSQRSGSTSAQDMVLSSGSGRRGH LLQPRSALWMERRG
Sbjct: 481 EYVRRQLFSSATNESGGRTSNYSQRSGSTSAQDMVLSSGSGRRGHHLLQPRSALWMERRG 540

Query: 541 DGGLGLPYSLRTLAASGEGSDNNRLVSEQIRNVLGLVRRGESLRVEDVMILDQSLFFGMA 600
           D GLGLPYSLRTLA SGEGSDNNRLVSEQIRNVLGLVRRGESLRVEDVMILDQSLFFGMA
Sbjct: 541 DSGLGLPYSLRTLATSGEGSDNNRLVSEQIRNVLGLVRRGESLRVEDVMILDQSLFFGMA 600

Query: 601 DIYDRHRDMRLDVDNMSYEELLALEERIGNVNTGLNEETIVARLKQKKHINATESQVEEE 660
           DIYDRHRDMRLDVDNMSYEELLALEERIGNVNTGL+EETIVARLK+ KH+NA E QVEEE
Sbjct: 601 DIYDRHRDMRLDVDNMSYEELLALEERIGNVNTGLSEETIVARLKRTKHVNAPEPQVEEE 660

Query: 661 PCCVCQEEYIEGEDIGTLECGHDFHTHCIKQWLMQKNLCPICKTTGLASRE 712
           PCCVCQEEY EGEDIG L+CGH FHT CIKQWLMQKN+CPICKTTG ASRE
Sbjct: 661 PCCVCQEEYKEGEDIGMLDCGHKFHTQCIKQWLMQKNICPICKTTGFASRE 711

BLAST of Lag0004255 vs. ExPASy TrEMBL
Match: A0A0A0LND7 (RING-type domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_2G356630 PE=4 SV=1)

HSP 1 Score: 1280.0 bits (3311), Expect = 0.0e+00
Identity = 649/713 (91.02%), Postives = 684/713 (95.93%), Query Frame = 0

Query: 1   MQGQRSSVGSLSETINFEHGSSSNNPGNQPVYWNNMWNPAENRIPDYLLPTSDINTGYVS 60
           MQGQRSS+GSLSETINFEHGSSSNNPGNQPVYWNN+WNPAENRIPDYLLPTSD+N+GYVS
Sbjct: 1   MQGQRSSIGSLSETINFEHGSSSNNPGNQPVYWNNVWNPAENRIPDYLLPTSDVNSGYVS 60

Query: 61  SVSHEQRSLSRWSLGEPSSCDMQTEALPDEQKAELGWSSVTRDADGPVTDNRLCEPSNNP 120
           SVSHEQRSLSRWSLGEPSSCDMQTE LPDEQKAE+GWSS+ RDADGPVT+NRLCEPSNNP
Sbjct: 61  SVSHEQRSLSRWSLGEPSSCDMQTEMLPDEQKAEIGWSSMARDADGPVTENRLCEPSNNP 120

Query: 121 SLGHVNLSPLIIQ--NSNSNAIPHNLNLNASFASHGGDNSRVNEGSNVYKSSGSEEGRIL 180
           SLGHVNLSPLIIQ  NSNSNAIPHNLNLN+SF SHGGDNSRVNEG+NVYKSSG EEGRIL
Sbjct: 121 SLGHVNLSPLIIQNSNSNSNAIPHNLNLNSSFVSHGGDNSRVNEGTNVYKSSGPEEGRIL 180

Query: 181 CSSASDPLLLPSGNSGLPVVANDGRAGSSLDGRRAPCKRKAIEGSVAQSSISGSSNYSQH 240
           CS+ASDPLLLPSGNSGLPVV NDGR GSSLDGRRAPCKRK+IEG+VAQSS+SGSSNYSQH
Sbjct: 181 CSNASDPLLLPSGNSGLPVVGNDGRPGSSLDGRRAPCKRKSIEGNVAQSSLSGSSNYSQH 240

Query: 241 IESGAETPLPVFPGRYNTGNRLTIPAPPARMNQASLRGAGELTSNSFSESIVAESSDSSQ 300
           IESG+E  LPVFPGRYNTG+RL+IP PPARMNQA +RGAGELTSNSFSESIVAESSDSSQ
Sbjct: 241 IESGSEAHLPVFPGRYNTGSRLSIPPPPARMNQAPVRGAGELTSNSFSESIVAESSDSSQ 300

Query: 301 RNYRIRISSSNAQDSFSSAGTTVRHPGASSSQLPARLLPAEHVLDLRPAPAVDNIPAQGQ 360
           RNYRIRISSSNA DSF+SAGT VRHPG  SSQLP RLLPAEH+LDLRPAPAVDN+ AQGQ
Sbjct: 301 RNYRIRISSSNAPDSFASAGTAVRHPGPPSSQLPGRLLPAEHILDLRPAPAVDNVSAQGQ 360

Query: 361 PIMIHVPALPRNLQPYRWNGSSTSRSGSSSSSAGERQVVQREEVRPSNMGRNVSEHPMFV 420
           PIMIHVPALPR+LQPYRWNGSS+SRSGSSSSSAGERQVVQREEVRPS+MGRNVSEHPMFV
Sbjct: 361 PIMIHVPALPRSLQPYRWNGSSSSRSGSSSSSAGERQVVQREEVRPSSMGRNVSEHPMFV 420

Query: 421 PANELRNLVRNPSSRGITSSNLSIPGNVASTSRVGSSSGVHPSAGPWMPPENSPTQFPRR 480
           PANELR LVR+PSSR + SSNLSIPGNVASTSRVGSSSGVHPS+GPW+PPE+SPTQFPRR
Sbjct: 421 PANELRGLVRSPSSRALASSNLSIPGNVASTSRVGSSSGVHPSSGPWIPPESSPTQFPRR 480

Query: 481 LTEYVRRQLFSSATNESGGRTSNYSQRSGSTSAQDMVLSSGSGRRGHGLLQPRSALWMER 540
           LTEYVRRQLF+SATNESGGRTSNYSQRSGSTSAQ+MVLSSGSGRRGH LLQPRSALWMER
Sbjct: 481 LTEYVRRQLFASATNESGGRTSNYSQRSGSTSAQEMVLSSGSGRRGHHLLQPRSALWMER 540

Query: 541 RGDGGLGLPYSLRTLAASGEGSDNNRLVSEQIRNVLGLVRRGESLRVEDVMILDQSLFFG 600
           RGD GLGLPYSLRTLA S EGSDNNRLVSE +RNVLGLVRRGESLRVEDVMILDQSLFFG
Sbjct: 541 RGDSGLGLPYSLRTLATSAEGSDNNRLVSEHLRNVLGLVRRGESLRVEDVMILDQSLFFG 600

Query: 601 MADIYDRHRDMRLDVDNMSYEELLALEERIGNVNTGLNEETIVARLKQKKHINATESQVE 660
           MADIYDRHRDMRLDVDNMSYEELLALEERIGNVNTGLNEETIVARLKQKK +NA +SQVE
Sbjct: 601 MADIYDRHRDMRLDVDNMSYEELLALEERIGNVNTGLNEETIVARLKQKKRVNAVDSQVE 660

Query: 661 EEPCCVCQEEYIEGEDIGTLECGHDFHTHCIKQWLMQKNLCPICKTTGLASRE 712
           EEPCCVCQEEY++GEDIGTLECGHDFHT CIKQWLMQKNLCPICKTTGLASRE
Sbjct: 661 EEPCCVCQEEYVDGEDIGTLECGHDFHTACIKQWLMQKNLCPICKTTGLASRE 713

BLAST of Lag0004255 vs. TAIR 10
Match: AT5G42940.1 (RING/U-box superfamily protein )

HSP 1 Score: 386.7 bits (992), Expect = 3.9e-107
Identity = 296/730 (40.55%), Postives = 408/730 (55.89%), Query Frame = 0

Query: 1   MQGQRSSVGSLSETINFEHGSSSNNPGNQPVYWNNMWNPAENRIPDYLLPTSDINTGYVS 60
           MQG+R+S+G LSE +NFEHGSSS+N      +W N+ +  +N + DY++  S+ NT   +
Sbjct: 1   MQGERASLGYLSEALNFEHGSSSSN--GVIDHWENIHSLGDNDLQDYMIANSESNTSLAN 60

Query: 61  SVSHEQRSLSRWSLGEPSSCDMQTEALP-DEQKAELGWSSVTRDADGPVTDNRLCEPSNN 120
           SV HEQ+ L R+SLGE SS   + EA   +EQ+ E      TR  DG    N +      
Sbjct: 61  SVYHEQQGLRRFSLGEASSSGTKDEASSHNEQRME------TRCFDG--RGNEI------ 120

Query: 121 PSLGHVNLSPLIIQNSNSNAIPHNLNLNASFASHGGDNSRVNEGSNVYKS-SGSEEGRIL 180
                ++L P+  Q S +N    N+NLNA +         ++E  N Y+  SG  E    
Sbjct: 121 -----IDLDPVFAQPSGTNQPVQNVNLNAEYI-------EIHEDINPYRGRSGFIEANGP 180

Query: 181 CSSASDPLLLPSGNSGLPVVANDGRAGSSLDGRRAPCKRKAIEGSVAQSSISGSSNYSQH 240
            +  S P        G     N    GSS++GRRA CKRKA+EGS++QSS  G  ++ Q 
Sbjct: 181 GTRVSQP--------GRSFEENGVGTGSSVEGRRASCKRKALEGSISQSSSGGYHDF-QR 240

Query: 241 IESGAETPLPVFPGRYNTGNRLTIPAPPARMNQASLRGAGELTSNSFSESIVAESSDSSQ 300
            ES + TP       +  GN L I        +  + G       S     V+  ++SS 
Sbjct: 241 GESSSWTPGSTV---FRPGNGLNISGSLDNGPRGMVSGTVPNFPVSAPNFPVSAIAESSS 300

Query: 301 RNYRIRISSSNAQDSFS----SAGTTVRHPGASSSQLPARLLPA-EHVLDLRPAPA-VDN 360
           RN  +R + S+ Q++ +    +AGT VR P   S    +R LPA +H LDLRP  + V +
Sbjct: 301 RNICVRSNPSDHQETVNPSTFAAGTVVRRPVPPSQLNLSRHLPADQHSLDLRPGQSFVVS 360

Query: 361 IPAQGQPIMIHVPALPRNLQPYRWNGSSTSRSGSSSSSAGERQVVQREEVRPSNMGRNVS 420
                 P+ I  P     L P+RW GSS    G+S+S+A   + +  +E R  ++  N  
Sbjct: 361 RNPNSTPVSI-PPGSRTMLPPFRWTGSSLV-GGTSNSTAPVERNLHLDETRSRSIPGNTL 420

Query: 421 EHPMFVPANELRNLVRNPSSRGITSSNLSIPGNVASTSRVGSSSGVHPSAGP-----WMP 480
           E PMF  A E+ N  R+ SSR +T+ NL+   + +S SR GS++ V P   P     W  
Sbjct: 421 EIPMFA-APEVGNFARSQSSRNVTNGNLN---SASSVSRTGSTTSVPPPPPPSSNLAWTS 480

Query: 481 PENSPTQFPRRL--TEYVRRQLFSS----ATNESGGRTSNYSQRSGSTSAQDMVLS-SGS 540
            +NSP    RR   +E  RR L SS    ATN+  G        +   S+  +VL   G 
Sbjct: 481 YQNSPHYQRRRTERSELARRSLLSSLAADATNQRSGDHPTLRSLAPPASSDGLVLQPGGD 540

Query: 541 GRRGHGLLQPRSALWMERRGDGGLGLPYSLRTLAASGEGSDNNRLVSEQIRNVLGLVRR- 600
             + H     R+    +R+GD  +G+P+ LR LAA+  G   +RL+  Q++NVL ++RR 
Sbjct: 541 NSQMHNRAYSRAGPLFDRQGDSVVGIPHPLRALAAASRG--RSRLMVSQMQNVLDVMRRD 600

Query: 601 --GESLRVEDVMILDQSLFFGMADIYDRHRDMRLDVDNMSYEELLALEERIGNVNTGLNE 660
               +LR+EDVM+L+ S+ F  A  +DR+RDMRLDVDNMSYEELLALEERIG+V TG+NE
Sbjct: 601 ANNNNLRLEDVMLLNHSVLFDGATGHDRYRDMRLDVDNMSYEELLALEERIGDVCTGVNE 660

Query: 661 ETIVARLKQKKHINATESQVEEEPCCVCQEEYIEGEDIGTLECGHDFHTHCIKQWLMQKN 708
           ETI  RLKQ+K+ + T+S  + EPCCVCQEEY EGED+GTLECGH+FH+ CIK+WL QKN
Sbjct: 661 ETISNRLKQRKYKSNTKSPQDAEPCCVCQEEYTEGEDMGTLECGHEFHSQCIKEWLKQKN 682

BLAST of Lag0004255 vs. TAIR 10
Match: AT1G45180.1 (RING/U-box superfamily protein )

HSP 1 Score: 360.1 bits (923), Expect = 4.0e-99
Identity = 275/715 (38.46%), Postives = 387/715 (54.13%), Query Frame = 0

Query: 1   MQGQRSSVGSLSETINFEHGSSSNNP-GNQPVYWNNMWNPAENRIPDYLLPTSDINTGYV 60
           MQG+R+S+GSLS+ +NFE GS+S+N   +Q + W N+ N  +N + DY+   +D N  + 
Sbjct: 1   MQGERASLGSLSKALNFERGSTSSNAVVDQQIRWENLHNYGDNDLQDYMSSAADTNPTFA 60

Query: 61  SSVSHEQRSLSRWSLGEPSSCDMQTEALPDEQKAELGWSSVTRDADGPVTDNRLCEPSNN 120
           +SV HE+R L R+++GE SS   + E     ++    W  + R            E   N
Sbjct: 61  NSVYHEKRGLQRFNIGEASSSGTKNEGASLTEQ----WKGIGR-----------FEEQRN 120

Query: 121 PSLGHVNLSPLIIQNSNSNAIPHNLNLNASFASHGGDNSRVNEGSNVYKSSGSEEGRILC 180
             L    LSPL +Q SN + +  ++NLNA +  H  D + V      ++ +G     +L 
Sbjct: 121 DKL---ELSPLFVQPSNGSRVVRDVNLNAEYNEHLEDMNPVTVHPGHFEVNGLRSEFLLD 180

Query: 181 SSASDPLLLPSGNSGLPVVANDGRAGSSLDGRRAPCKRKAIEGSVAQSSISGSSNYSQHI 240
           +S                     RAGSS+DGRRA CKRKA++ S  QSS +G     Q  
Sbjct: 181 NSV--------------------RAGSSVDGRRASCKRKALDASGGQSSSTGGFREFQRG 240

Query: 241 ESGAETPLPVFPGRYNTGNRLTIPAPPARMNQASLRGAGELTSNSFSESIVAESSDSSQR 300
            S +    P +     T N L I     R      RG   L S++         ++SS R
Sbjct: 241 VSSSWISGPTYYSPAMTANDLNISLDHGR------RG---LVSSAVPNLSPPTITESSSR 300

Query: 301 NYRIRISSSNAQDSFSSAGTTVRHPGASSSQLPARLLPAEH-VLDLRPAPAVDNIPAQG- 360
           NY + ++ +  Q++ S+   ++  PG         L+PA+H  + +R   A+ N  +Q  
Sbjct: 301 NYPVWVNPTYQQETVSNFAPSLNSPG---------LIPADHQQISMRYGHALGNFASQNP 360

Query: 361 QPIMIHVPALPRNLQPYRWNGSSTSRSGSSSSSAG-ERQVVQREEVRPSNMGRNVSEHPM 420
                H+P + RN   ++WN S  +   SSSS+   +R V+ R   R  +   N  E P+
Sbjct: 361 NAPATHMPPVSRN--TFQWNTSPVAAVISSSSATPVDRNVIHRNATRQRS---NTLEIPL 420

Query: 421 FVPANELRNLVRNPSSRGITSSNLSIPGNVASTSRVGSSSGVHPS-AGPWMPP-ENSPTQ 480
           FVPA ELRN+     + G  S N S   +VAS+S   S + V PS + P + P +N+   
Sbjct: 421 FVPAPELRNV-----AHGHISRNASGARHVASSS---SRTSVQPSPSSPALTPYQNNSLH 480

Query: 481 FPRRLTEYVRRQLFSSATNESGGRTSNYSQRSGSTSAQDMVLSSGSGRRG--HGLLQPRS 540
             RRL+E  RR L SS   +     S     +   S  + VL SG       H     R+
Sbjct: 481 NQRRLSENFRRSLLSSLVTQQRAARS----LAHPASPNEHVLQSGGDNTSQVHNRASSRA 540

Query: 541 ALWMERRGDGGLGLPYSLRTLAASGEGSDNNRLVSEQIRNVLGLVRRGESLRVEDVMILD 600
                R+G    G+ +SLR LA++  G    R+ + +IRN+L  +RR  +LR+EDVM+L+
Sbjct: 541 G---PRQGQDATGISHSLRGLASTSRG--RTRMGASEIRNILEHMRRAGNLRLEDVMLLN 600

Query: 601 QSLFFGMADIYDRHRDMRLDVDNMSYEELLALEERIGNVNTGLNEETIVARLKQKKHINA 660
           QS+  G ADI+DR+RDMRLDVDNM+YEELL+LEERIG+V TGLNEETI  RLKQ+K+ ++
Sbjct: 601 QSIMLGAADIHDRYRDMRLDVDNMTYEELLSLEERIGDVCTGLNEETISNRLKQQKYKSS 637

Query: 661 TESQVEEEPCCVCQEEYIEGEDIGTLECGHDFHTHCIKQWLMQKNLCPICKTTGL 708
           T S  E EPCCVCQEEY E E+IG LECGHDFH+ CIK+WL QKNLCPICKTTGL
Sbjct: 661 TRSSQEVEPCCVCQEEYKEEEEIGRLECGHDFHSQCIKEWLKQKNLCPICKTTGL 637

BLAST of Lag0004255 vs. TAIR 10
Match: AT4G34040.1 (RING/U-box superfamily protein )

HSP 1 Score: 302.8 bits (774), Expect = 7.5e-82
Identity = 265/727 (36.45%), Postives = 360/727 (49.52%), Query Frame = 0

Query: 1   MQGQRSSVGSLSETINFEHGSSSNNPGNQPVYWNNMWNPAENRIPDYLLPTSDINTGYVS 60
           MQG RS+ G  S  IN+  G    +  N     NN+ NP + + P+    T      Y S
Sbjct: 1   MQGPRST-GDSSTGINYADGEPICST-NSETTSNNILNPVDVQFPNN--TTGSGRPTYAS 60

Query: 61  SVSHEQRSLSRWSLGEPSSCDMQTEALPDEQKAELGWSSVTRDADGPVTDNRLCEPSNNP 120
           S SH  ++ + WS GE SS               LG  S   +++G  TD +L       
Sbjct: 61  SSSHVVQNHNWWSFGESSS--------------RLG-PSDHLNSNGSKTDRQLLSDGYGF 120

Query: 121 SLGHVNLSPLIIQNSNSNAIPHNLNLNASFASHGGDNSRVNEGSNVYKSSGSEEGRILCS 180
             G   +            +P    L  S +SH    S VN G ++   SG +   ++  
Sbjct: 121 EEGQSGM-----------LLPGESFLRGSSSSH--MLSHVNLGKDMDIGSGLQTSGVVIR 180

Query: 181 SASDPLLLPSGNSGLPVVANDGRAGSSLDGRRAPCKRKAIEGSVAQSSISGSSNYSQHIE 240
             +    L  G+S           GSSL G  + CKRKA+EG+ + S    S       E
Sbjct: 181 HNNCETSL--GSSSQTAEERSSGPGSSLGGLGSSCKRKALEGAPSHSFPGESHGCFFQTE 240

Query: 241 SGAETPLPVFPGRYNTGNRLTIPAP----PARMNQA-------SLRGAGELTSNSFSESI 300
           +GA         +Y+  + L++  P    P   NQ+        L G   +T+++F  + 
Sbjct: 241 NGAWNE---GLAQYDASSSLSLSMPSQNSPNVNNQSGLPEPRFGLGGGRAVTASAFPSTR 300

Query: 301 VAESSDSSQRNYRIRISSSNAQDSFSSAGTTVRHPGASSSQLPARLLPAEHVLDLRPAPA 360
             E+     R         +   SF+ +G++VR       QLPA   P    LD R  P 
Sbjct: 301 STETISRPGRRLNPGQPPESVAFSFTQSGSSVR----QQQQLPA-TSPFVDPLDARAIPV 360

Query: 361 V-DNIPAQGQPIMIHVPALPRNLQPYRWNGSSTSRSGSSSSSAGERQVVQREEVRPSNMG 420
              +    GQP MIH+PAL RN+  + W+ SS+SR+ S          +  E + P +  
Sbjct: 361 TGSSSSGDGQPSMIHLPALTRNIHQFAWSASSSSRANS----------MPEEGLSPWDAP 420

Query: 421 RNVSEHPMF-VPANELRNLVRNPSSRGITSSNLSIPGNVASTSRVGSSSGVH--PSAGPW 480
           R  SE P+F  PANE RN V++      T  N S  G+     R GSSSG+H       W
Sbjct: 421 RINSEQPVFTTPANETRNPVQDQFCWSFTRGNPSTSGDSPFVPRAGSSSGIHGLQPNPTW 480

Query: 481 MPPENSPTQFPRRLTEYVRRQLFSSATNESG--GRTSNYSQRSGSTSAQDMVLSSGSGRR 540
           + P N       R++E     LF S  +ES   G +        S S+ +    SGS  R
Sbjct: 481 VTPHNQ-----SRISEVAPWSLFPSIESESATHGASLPLLPTGPSVSSNEAAAPSGSSSR 540

Query: 541 GHGLLQPRSALWMERRGDGGLGLPYSLRTLAASGEGSDNNRLVSEQIRNVLGLVRRGESL 600
            H   Q RS L +ER+ D  L L +  R+LAA  +G   NRL+SE IR VL  +RRGE+L
Sbjct: 541 SHRSRQRRSGLLLERQND-HLHLRHLGRSLAADNDG--RNRLISE-IRQVLSAMRRGENL 600

Query: 601 RVEDVMILDQSLFFGMADIYDRHRDMRLDVDNMSYEELLALEERIGNVNTGLNEETIVAR 660
           R ED M+ D  ++ GMA+++DRHRDMRLDVDNMSYEELLAL ERIG+V+TGL+EE I+  
Sbjct: 601 RFEDYMVFDPLIYQGMAEMHDRHRDMRLDVDNMSYEELLALGERIGDVSTGLSEEVILKV 660

Query: 661 LKQKKHI-NATESQVEEEPCCVCQEEYIEGEDIGTLECGHDFHTHCIKQWLMQKNLCPIC 710
           +KQ KH  +A  S  + EPCCVCQEEY EG+D+GTL CGH+FHT C+KQWLM KNLCPIC
Sbjct: 661 MKQHKHTSSAAGSHQDMEPCCVCQEEYAEGDDLGTLGCGHEFHTACVKQWLMLKNLCPIC 666

BLAST of Lag0004255 vs. TAIR 10
Match: AT2G15530.1 (RING/U-box superfamily protein )

HSP 1 Score: 275.0 bits (702), Expect = 1.7e-73
Identity = 247/754 (32.76%), Postives = 360/754 (47.75%), Query Frame = 0

Query: 1   MQGQRSSVGSLSETINFEHGSSSNNPGNQPVY-----WNNMWNPAENRIPDYLLPTSDIN 60
           MQG RS  GS +E          N    + +Y      N M NPA+   P+   P+    
Sbjct: 4   MQGPRSIGGSSTEV---------NQVDGESIYCTETSLNTMLNPADTGFPNNSTPSG--R 63

Query: 61  TGYVSSVSHEQRSLSRWSLGEPSSCDMQTEAL------------PDEQKAELGWSSVTRD 120
             Y SS SH  +  + W  GE SS    ++ +             D     +G+ S  R 
Sbjct: 64  PTYASSSSHAAQDHTWWRFGESSSIPGPSDQVNSIGIKTSHQLPQDGTHHFVGYGSEGRQ 123

Query: 121 A--DGPVTDNRLCEPSNNPSLGHVNLSPLIIQNSNSNAIPHNLNLNASFASH--GGDNSR 180
              +G + D  +   S      H+   P  ++ S+SN +P +++++    S       S 
Sbjct: 124 TGLNGMMVDGGVHAGS------HIRNVPSFLRGSSSNPMPQHVDMSMDMDSDNCNAQTSG 183

Query: 181 VNEGSNVYKSSGSEEGRILCSSASDPLLLPSGNSGLPVVANDGRAGSSLDGRRAPCKRKA 240
           V    N Y SS     +    S+S P                    S   G  + CKRKA
Sbjct: 184 VVIRHNSYGSSLGSSVQAAGESSSGP-------------------ASPFGGWGSSCKRKA 243

Query: 241 IEGSVAQSSISGSSNYSQHIESGAETPLPVFPGRYNTGNRLTIPAPP----------ARM 300
           +EGS +      + N     E+ A         +Y   + L++  P            R 
Sbjct: 244 LEGSPSHYFSGETPNRIVQTENSAS---HASLSQYGASSSLSLATPSQSSPNVTNHFGRT 303

Query: 301 NQASLRGAGE-LTSNSFSESIVAESSDSSQRNYRIRISSSNAQDSFSSAGTTVRHPGASS 360
            Q    G G  + +++F  +   ++   + R    R    +   S S  GT+VR  G+  
Sbjct: 304 EQMFGSGGGRAVAASAFHSTRNTDTLSRAGRRLNPRQPQESVAFSVSHGGTSVRPTGSLQ 363

Query: 361 SQLPARLLPAEHVLDLRPAPAVD-NIPAQGQPIMIHVPALPRNLQPYRWNGSSTSRSGSS 420
             LP    P     D+R +     +   + Q  ++H+PAL RN+  Y W+ S +SR+ S+
Sbjct: 364 QNLPLN-SPFVDPPDVRSSSITSGSNTGENQTNIVHLPALTRNIHQYAWDASFSSRA-SN 423

Query: 421 SSSAGERQVVQREEVRPS-NMGRNVSEHPMFVPANELRNLVRN-----PSSRGITSSNLS 480
            S  G    +  E + P     R+  E P+F PA ++R  V +       S G +  +L 
Sbjct: 424 PSGIG----MPAERLGPQWETPRSNQEQPLFAPATDMRQPVHDLWNFARGSPGSSVDSLF 483

Query: 481 IPGNVASTSRVGSSSGVH-PSAGP-WMPPENSPTQFPRRLTEYVRRQLFSS--ATNESGG 540
           +P       R G SS +H P   P W+PP+N+P   P R +E     LF S  + + S G
Sbjct: 484 VP-------RAGPSSAIHTPQPNPTWIPPQNAPPHNPSRTSELSPWSLFPSIESPSASHG 543

Query: 541 RTSNYSQRSGSTSAQDMVLSSGSGRRGHGLLQPRSALWMERRGDGGLGLPYSLRTLAASG 600
                     S S+ ++ + S S  R H     RS L +ER+ +  L L +  R+LAA G
Sbjct: 544 GPLPLLPAGPSVSSNEVTMPSSSNSRSHRSRHRRSGLLLERQNE-LLHLRHIGRSLAADG 603

Query: 601 EGSDNNRLVSEQIRNVLGLVRRGESLRVEDVMILDQSLFFGMADIYDRHRDMRLDVDNMS 660
            G   N+++SE IR VL  +RRGE+LRVED M+ D  ++ GM D++DRHR+MRLDVDNMS
Sbjct: 604 NG--RNQIISE-IRQVLHAMRRGENLRVEDYMVFDPLIYQGMTDMHDRHREMRLDVDNMS 663

Query: 661 YEELLALEERIGNVNTGLNEETIVARLKQKKHINATESQVE----EEPCCVCQEEYIEGE 708
           YEELLAL ERIG+V+TGL+EE I+  +KQ KH +++ S VE     EPCC+CQEEY+EG+
Sbjct: 664 YEELLALGERIGDVSTGLSEEVILKAMKQHKHTSSSPSSVELHQNIEPCCICQEEYVEGD 701

BLAST of Lag0004255 vs. TAIR 10
Match: AT2G15530.2 (RING/U-box superfamily protein )

HSP 1 Score: 275.0 bits (702), Expect = 1.7e-73
Identity = 247/754 (32.76%), Postives = 360/754 (47.75%), Query Frame = 0

Query: 1   MQGQRSSVGSLSETINFEHGSSSNNPGNQPVY-----WNNMWNPAENRIPDYLLPTSDIN 60
           MQG RS  GS +E          N    + +Y      N M NPA+   P+   P+    
Sbjct: 4   MQGPRSIGGSSTEV---------NQVDGESIYCTETSLNTMLNPADTGFPNNSTPSG--R 63

Query: 61  TGYVSSVSHEQRSLSRWSLGEPSSCDMQTEAL------------PDEQKAELGWSSVTRD 120
             Y SS SH  +  + W  GE SS    ++ +             D     +G+ S  R 
Sbjct: 64  PTYASSSSHAAQDHTWWRFGESSSIPGPSDQVNSIGIKTSHQLPQDGTHHFVGYGSEGRQ 123

Query: 121 A--DGPVTDNRLCEPSNNPSLGHVNLSPLIIQNSNSNAIPHNLNLNASFASH--GGDNSR 180
              +G + D  +   S      H+   P  ++ S+SN +P +++++    S       S 
Sbjct: 124 TGLNGMMVDGGVHAGS------HIRNVPSFLRGSSSNPMPQHVDMSMDMDSDNCNAQTSG 183

Query: 181 VNEGSNVYKSSGSEEGRILCSSASDPLLLPSGNSGLPVVANDGRAGSSLDGRRAPCKRKA 240
           V    N Y SS     +    S+S P                    S   G  + CKRKA
Sbjct: 184 VVIRHNSYGSSLGSSVQAAGESSSGP-------------------ASPFGGWGSSCKRKA 243

Query: 241 IEGSVAQSSISGSSNYSQHIESGAETPLPVFPGRYNTGNRLTIPAPP----------ARM 300
           +EGS +      + N     E+ A         +Y   + L++  P            R 
Sbjct: 244 LEGSPSHYFSGETPNRIVQTENSAS---HASLSQYGASSSLSLATPSQSSPNVTNHFGRT 303

Query: 301 NQASLRGAGE-LTSNSFSESIVAESSDSSQRNYRIRISSSNAQDSFSSAGTTVRHPGASS 360
            Q    G G  + +++F  +   ++   + R    R    +   S S  GT+VR  G+  
Sbjct: 304 EQMFGSGGGRAVAASAFHSTRNTDTLSRAGRRLNPRQPQESVAFSVSHGGTSVRPTGSLQ 363

Query: 361 SQLPARLLPAEHVLDLRPAPAVD-NIPAQGQPIMIHVPALPRNLQPYRWNGSSTSRSGSS 420
             LP    P     D+R +     +   + Q  ++H+PAL RN+  Y W+ S +SR+ S+
Sbjct: 364 QNLPLN-SPFVDPPDVRSSSITSGSNTGENQTNIVHLPALTRNIHQYAWDASFSSRA-SN 423

Query: 421 SSSAGERQVVQREEVRPS-NMGRNVSEHPMFVPANELRNLVRN-----PSSRGITSSNLS 480
            S  G    +  E + P     R+  E P+F PA ++R  V +       S G +  +L 
Sbjct: 424 PSGIG----MPAERLGPQWETPRSNQEQPLFAPATDMRQPVHDLWNFARGSPGSSVDSLF 483

Query: 481 IPGNVASTSRVGSSSGVH-PSAGP-WMPPENSPTQFPRRLTEYVRRQLFSS--ATNESGG 540
           +P       R G SS +H P   P W+PP+N+P   P R +E     LF S  + + S G
Sbjct: 484 VP-------RAGPSSAIHTPQPNPTWIPPQNAPPHNPSRTSELSPWSLFPSIESPSASHG 543

Query: 541 RTSNYSQRSGSTSAQDMVLSSGSGRRGHGLLQPRSALWMERRGDGGLGLPYSLRTLAASG 600
                     S S+ ++ + S S  R H     RS L +ER+ +  L L +  R+LAA G
Sbjct: 544 GPLPLLPAGPSVSSNEVTMPSSSNSRSHRSRHRRSGLLLERQNE-LLHLRHIGRSLAADG 603

Query: 601 EGSDNNRLVSEQIRNVLGLVRRGESLRVEDVMILDQSLFFGMADIYDRHRDMRLDVDNMS 660
            G   N+++SE IR VL  +RRGE+LRVED M+ D  ++ GM D++DRHR+MRLDVDNMS
Sbjct: 604 NG--RNQIISE-IRQVLHAMRRGENLRVEDYMVFDPLIYQGMTDMHDRHREMRLDVDNMS 663

Query: 661 YEELLALEERIGNVNTGLNEETIVARLKQKKHINATESQVE----EEPCCVCQEEYIEGE 708
           YEELLAL ERIG+V+TGL+EE I+  +KQ KH +++ S VE     EPCC+CQEEY+EG+
Sbjct: 664 YEELLALGERIGDVSTGLSEEVILKAMKQHKHTSSSPSSVELHQNIEPCCICQEEYVEGD 701