Lag0004220 (gene) Sponge gourd (AG‐4) v1

Overview
NameLag0004220
Typegene
OrganismLuffa acutangula (Sponge gourd (AG‐4) v1)
DescriptionAB hydrolase-1 domain-containing protein
Locationchr6: 2023644 .. 2024528 (-)
RNA-Seq ExpressionLag0004220
SyntenyLag0004220
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: polypeptideexonCDS
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGGTTAACAACGCCCTTCTGGAGGCCCTGAATGTCCGTGTCCTCGGCACCGGCGACCGCTTCCTTGTCCTCGCCCATGGCTTCGGCACCGACCAGTCCGCTTGGCAACTCGTTTACCCTTCCTTCACCCCTTACTACCGCGTCATCCTCTACGACCTTGTCTGCGCCGGCAGCGTCAACCCCGACTTCTTCGATTTCTCCCGCTACACCACCCTCGACGCCTTCGTCGACGACCTCATCTCCATCCTCGACTCCCTCCACGTCCACCGCTGCGCCTTCGTCGGCCACTCCGTCTCCGCCATGGTCGGCATCCTCGCCTCCATCCGCCGCCCCGACCTCTTCTCCAAGCTCGTCTTAATCGGCGCCTCCCCAAGGTCCTCCCTCTACCTACTACTACTACTCTGTTTTTAGTTTTCTCCCCCTAGGCCCTAGCTAATTTCTTTTTTTTTTTTCAGGTTCCTCAACGACGGCGACTACCACGGCGGCTTCGAGCAGAGCGAGATTGACAGGGTCTTCGCCGCAATGAAGGCCAATTACCAAGCCTGGGTCAACGGATTCGCCCCTCTCGCCGTCGGTGCCGATGTTCCCGCCGCCGTACAGGAATTCAGCCGGACTCTCTTCAACATGAGGCCCGACATTTCCCTCTTCGTCTCCAAGGTCATCTTCAGCAGCGATCTCCGGGGAGTCCTCGGCCTCGTCAAAGTCCCCTGCTGCATAATTCAAACCGCCCAAGACGTCTCCGTTCCGGCCTCCGTCGCCATCTACCTCAGAGACCACCTCGGCGGCCGGAACACCGTCGAGATGCTCGACACCGAGGGCCACCTACCCCATCTCAGCGCCCCTCAGCTACTCGTCCGCAAGCTCCGCCGCGCCCTTGCTCGCTGA

mRNA sequence

ATGGTTAACAACGCCCTTCTGGAGGCCCTGAATGTCCGTGTCCTCGGCACCGGCGACCGCTTCCTTGTCCTCGCCCATGGCTTCGGCACCGACCAGTCCGCTTGGCAACTCGTTTACCCTTCCTTCACCCCTTACTACCGCGTCATCCTCTACGACCTTGTCTGCGCCGGCAGCGTCAACCCCGACTTCTTCGATTTCTCCCGCTACACCACCCTCGACGCCTTCGTCGACGACCTCATCTCCATCCTCGACTCCCTCCACGTCCACCGCTGCGCCTTCGTCGGCCACTCCGTCTCCGCCATGGTCGGCATCCTCGCCTCCATCCGCCGCCCCGACCTCTTCTCCAAGCTCGTCTTAATCGGCGCCTCCCCAAGGTTCCTCAACGACGGCGACTACCACGGCGGCTTCGAGCAGAGCGAGATTGACAGGGTCTTCGCCGCAATGAAGGCCAATTACCAAGCCTGGGTCAACGGATTCGCCCCTCTCGCCGTCGGTGCCGATGTTCCCGCCGCCGTACAGGAATTCAGCCGGACTCTCTTCAACATGAGGCCCGACATTTCCCTCTTCGTCTCCAAGGTCATCTTCAGCAGCGATCTCCGGGGAGTCCTCGGCCTCGTCAAAGTCCCCTGCTGCATAATTCAAACCGCCCAAGACGTCTCCGTTCCGGCCTCCGTCGCCATCTACCTCAGAGACCACCTCGGCGGCCGGAACACCGTCGAGATGCTCGACACCGAGGGCCACCTACCCCATCTCAGCGCCCCTCAGCTACTCGTCCGCAAGCTCCGCCGCGCCCTTGCTCGCTGA

Coding sequence (CDS)

ATGGTTAACAACGCCCTTCTGGAGGCCCTGAATGTCCGTGTCCTCGGCACCGGCGACCGCTTCCTTGTCCTCGCCCATGGCTTCGGCACCGACCAGTCCGCTTGGCAACTCGTTTACCCTTCCTTCACCCCTTACTACCGCGTCATCCTCTACGACCTTGTCTGCGCCGGCAGCGTCAACCCCGACTTCTTCGATTTCTCCCGCTACACCACCCTCGACGCCTTCGTCGACGACCTCATCTCCATCCTCGACTCCCTCCACGTCCACCGCTGCGCCTTCGTCGGCCACTCCGTCTCCGCCATGGTCGGCATCCTCGCCTCCATCCGCCGCCCCGACCTCTTCTCCAAGCTCGTCTTAATCGGCGCCTCCCCAAGGTTCCTCAACGACGGCGACTACCACGGCGGCTTCGAGCAGAGCGAGATTGACAGGGTCTTCGCCGCAATGAAGGCCAATTACCAAGCCTGGGTCAACGGATTCGCCCCTCTCGCCGTCGGTGCCGATGTTCCCGCCGCCGTACAGGAATTCAGCCGGACTCTCTTCAACATGAGGCCCGACATTTCCCTCTTCGTCTCCAAGGTCATCTTCAGCAGCGATCTCCGGGGAGTCCTCGGCCTCGTCAAAGTCCCCTGCTGCATAATTCAAACCGCCCAAGACGTCTCCGTTCCGGCCTCCGTCGCCATCTACCTCAGAGACCACCTCGGCGGCCGGAACACCGTCGAGATGCTCGACACCGAGGGCCACCTACCCCATCTCAGCGCCCCTCAGCTACTCGTCCGCAAGCTCCGCCGCGCCCTTGCTCGCTGA

Protein sequence

MVNNALLEALNVRVLGTGDRFLVLAHGFGTDQSAWQLVYPSFTPYYRVILYDLVCAGSVNPDFFDFSRYTTLDAFVDDLISILDSLHVHRCAFVGHSVSAMVGILASIRRPDLFSKLVLIGASPRFLNDGDYHGGFEQSEIDRVFAAMKANYQAWVNGFAPLAVGADVPAAVQEFSRTLFNMRPDISLFVSKVIFSSDLRGVLGLVKVPCCIIQTAQDVSVPASVAIYLRDHLGGRNTVEMLDTEGHLPHLSAPQLLVRKLRRALAR
Homology
BLAST of Lag0004220 vs. NCBI nr
Match: XP_008444562.1 (PREDICTED: strigolactone esterase D14 [Cucumis melo] >KAA0060949.1 strigolactone esterase D14 [Cucumis melo var. makuwa])

HSP 1 Score: 528.1 bits (1359), Expect = 4.4e-146
Identity = 261/267 (97.75%), Postives = 266/267 (99.63%), Query Frame = 0

Query: 1   MVNNALLEALNVRVLGTGDRFLVLAHGFGTDQSAWQLVYPSFTPYYRVILYDLVCAGSVN 60
           MVNNALLEALNVRVLGTGDRFLVLAHGFGTDQSAWQLVYPSFTPYYRVILYDLVCAGSVN
Sbjct: 1   MVNNALLEALNVRVLGTGDRFLVLAHGFGTDQSAWQLVYPSFTPYYRVILYDLVCAGSVN 60

Query: 61  PDFFDFSRYTTLDAFVDDLISILDSLHVHRCAFVGHSVSAMVGILASIRRPDLFSKLVLI 120
           PDFFDFSRYTTLDAFVDDLISILDSLHVHRCAFVGHSVSAMVGILASIRRP+LFSKL+LI
Sbjct: 61  PDFFDFSRYTTLDAFVDDLISILDSLHVHRCAFVGHSVSAMVGILASIRRPELFSKLILI 120

Query: 121 GASPRFLNDGDYHGGFEQSEIDRVFAAMKANYQAWVNGFAPLAVGADVPAAVQEFSRTLF 180
           GASPRFLNDGDYHGGFEQSEIDRVFAAMKANYQ+WVNGFAPLAVGADVPAAVQEFSRTLF
Sbjct: 121 GASPRFLNDGDYHGGFEQSEIDRVFAAMKANYQSWVNGFAPLAVGADVPAAVQEFSRTLF 180

Query: 181 NMRPDISLFVSKVIFSSDLRGVLGLVKVPCCIIQTAQDVSVPASVAIYLRDHLGGRNTVE 240
           NMRPDISLFVSKVIFSSDLRGVLGLVKVPCCIIQTAQDVSVP SVAIYLRDHLGGRNT+E
Sbjct: 181 NMRPDISLFVSKVIFSSDLRGVLGLVKVPCCIIQTAQDVSVPTSVAIYLRDHLGGRNTIE 240

Query: 241 MLDTEGHLPHLSAPQLLVRKLRRALAR 268
           MLDTEGHLPHLSAPQLLVRKLRRAL+R
Sbjct: 241 MLDTEGHLPHLSAPQLLVRKLRRALSR 267

BLAST of Lag0004220 vs. NCBI nr
Match: XP_004142890.1 (strigolactone esterase RMS3 [Cucumis sativus] >KGN62452.1 hypothetical protein Csa_018792 [Cucumis sativus])

HSP 1 Score: 526.9 bits (1356), Expect = 9.9e-146
Identity = 260/267 (97.38%), Postives = 266/267 (99.63%), Query Frame = 0

Query: 1   MVNNALLEALNVRVLGTGDRFLVLAHGFGTDQSAWQLVYPSFTPYYRVILYDLVCAGSVN 60
           MVNNALLEALNVRVLGTGDRFLVLAHGFGTDQSAWQLVYPSFTPYYRVILYDLVCAGSVN
Sbjct: 1   MVNNALLEALNVRVLGTGDRFLVLAHGFGTDQSAWQLVYPSFTPYYRVILYDLVCAGSVN 60

Query: 61  PDFFDFSRYTTLDAFVDDLISILDSLHVHRCAFVGHSVSAMVGILASIRRPDLFSKLVLI 120
           PDFFDFSRYTTLDAFVDDLISILDSLHVHRCAFVGHSVSAMVGILASIRRP+LFSKL+LI
Sbjct: 61  PDFFDFSRYTTLDAFVDDLISILDSLHVHRCAFVGHSVSAMVGILASIRRPELFSKLILI 120

Query: 121 GASPRFLNDGDYHGGFEQSEIDRVFAAMKANYQAWVNGFAPLAVGADVPAAVQEFSRTLF 180
           GASPRFLNDGDYHGGFEQ+EIDRVFAAMKANYQ+WVNGFAPLAVGADVPAAVQEFSRTLF
Sbjct: 121 GASPRFLNDGDYHGGFEQNEIDRVFAAMKANYQSWVNGFAPLAVGADVPAAVQEFSRTLF 180

Query: 181 NMRPDISLFVSKVIFSSDLRGVLGLVKVPCCIIQTAQDVSVPASVAIYLRDHLGGRNTVE 240
           NMRPDISLFVSKVIFSSDLRGVLGLVKVPCCIIQTAQDVSVP SVAIYLRDHLGGRNT+E
Sbjct: 181 NMRPDISLFVSKVIFSSDLRGVLGLVKVPCCIIQTAQDVSVPTSVAIYLRDHLGGRNTIE 240

Query: 241 MLDTEGHLPHLSAPQLLVRKLRRALAR 268
           MLDTEGHLPHLSAPQLLVRKLRRAL+R
Sbjct: 241 MLDTEGHLPHLSAPQLLVRKLRRALSR 267

BLAST of Lag0004220 vs. NCBI nr
Match: XP_038886523.1 (strigolactone esterase D14 [Benincasa hispida])

HSP 1 Score: 523.9 bits (1348), Expect = 8.3e-145
Identity = 260/267 (97.38%), Postives = 264/267 (98.88%), Query Frame = 0

Query: 1   MVNNALLEALNVRVLGTGDRFLVLAHGFGTDQSAWQLVYPSFTPYYRVILYDLVCAGSVN 60
           M NNALLEALNVRVLGTGDR LVLAHGFGTDQSAWQLVYPSFTPYYRVILYDLVCAGSVN
Sbjct: 1   MANNALLEALNVRVLGTGDRCLVLAHGFGTDQSAWQLVYPSFTPYYRVILYDLVCAGSVN 60

Query: 61  PDFFDFSRYTTLDAFVDDLISILDSLHVHRCAFVGHSVSAMVGILASIRRPDLFSKLVLI 120
           PDFFDFSRYTTLDAFVDDLISILDSLHVHRCAFVGHSVSAMVGILASIRRP+LFSKL+LI
Sbjct: 61  PDFFDFSRYTTLDAFVDDLISILDSLHVHRCAFVGHSVSAMVGILASIRRPELFSKLILI 120

Query: 121 GASPRFLNDGDYHGGFEQSEIDRVFAAMKANYQAWVNGFAPLAVGADVPAAVQEFSRTLF 180
           GASPRFLNDGDYHGGFEQSEIDRVFAAM+ANYQAWVNGFAPLAVGADVPAAVQEFSRTLF
Sbjct: 121 GASPRFLNDGDYHGGFEQSEIDRVFAAMRANYQAWVNGFAPLAVGADVPAAVQEFSRTLF 180

Query: 181 NMRPDISLFVSKVIFSSDLRGVLGLVKVPCCIIQTAQDVSVPASVAIYLRDHLGGRNTVE 240
           NMRPDISLFVSKVIFSSDLRGVLGLVKVPCCIIQTAQDVSVP SVAIYLRDHLGGRNTVE
Sbjct: 181 NMRPDISLFVSKVIFSSDLRGVLGLVKVPCCIIQTAQDVSVPTSVAIYLRDHLGGRNTVE 240

Query: 241 MLDTEGHLPHLSAPQLLVRKLRRALAR 268
           MLDTEGHLPHLSAPQLLVRKLRRAL+R
Sbjct: 241 MLDTEGHLPHLSAPQLLVRKLRRALSR 267

BLAST of Lag0004220 vs. NCBI nr
Match: XP_023546980.1 (strigolactone esterase RMS3 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 516.2 bits (1328), Expect = 1.7e-142
Identity = 258/267 (96.63%), Postives = 262/267 (98.13%), Query Frame = 0

Query: 1   MVNNALLEALNVRVLGTGDRFLVLAHGFGTDQSAWQLVYPSFTPYYRVILYDLVCAGSVN 60
           MVNNALL+A NVRVLGTGDR LVLAHGFGTDQSAWQLVYPSFTPYYRVILYDLVCAGSVN
Sbjct: 1   MVNNALLDAFNVRVLGTGDRVLVLAHGFGTDQSAWQLVYPSFTPYYRVILYDLVCAGSVN 60

Query: 61  PDFFDFSRYTTLDAFVDDLISILDSLHVHRCAFVGHSVSAMVGILASIRRPDLFSKLVLI 120
           PD FDFSRYTTLDAFVDDLISILDSL V RCAFVGHSVSAMVGILASIRRP+LF+KLVLI
Sbjct: 61  PDHFDFSRYTTLDAFVDDLISILDSLDVQRCAFVGHSVSAMVGILASIRRPELFTKLVLI 120

Query: 121 GASPRFLNDGDYHGGFEQSEIDRVFAAMKANYQAWVNGFAPLAVGADVPAAVQEFSRTLF 180
           GASPRFLNDGDYHGGFEQSEIDRVFAAMKANYQAWVNGFAPLAVGADVPAAVQEFSRTLF
Sbjct: 121 GASPRFLNDGDYHGGFEQSEIDRVFAAMKANYQAWVNGFAPLAVGADVPAAVQEFSRTLF 180

Query: 181 NMRPDISLFVSKVIFSSDLRGVLGLVKVPCCIIQTAQDVSVPASVAIYLRDHLGGRNTVE 240
           NMRPDISLFVSKVIFSSDLRGVLGLVKVPCCI+QTAQDVSVPASVAIYLRDHLGGRNTVE
Sbjct: 181 NMRPDISLFVSKVIFSSDLRGVLGLVKVPCCILQTAQDVSVPASVAIYLRDHLGGRNTVE 240

Query: 241 MLDTEGHLPHLSAPQLLVRKLRRALAR 268
           MLDTEGHLPHLSAPQLLVRKLRRALAR
Sbjct: 241 MLDTEGHLPHLSAPQLLVRKLRRALAR 267

BLAST of Lag0004220 vs. NCBI nr
Match: XP_022996864.1 (strigolactone esterase RMS3 [Cucurbita maxima])

HSP 1 Score: 514.2 bits (1323), Expect = 6.6e-142
Identity = 257/267 (96.25%), Postives = 261/267 (97.75%), Query Frame = 0

Query: 1   MVNNALLEALNVRVLGTGDRFLVLAHGFGTDQSAWQLVYPSFTPYYRVILYDLVCAGSVN 60
           MVNNALL+A NVRVLGTGDR LVLAHGFGTDQSAWQLVYPSFTPYYRVILYDLVCAGSVN
Sbjct: 1   MVNNALLDAFNVRVLGTGDRVLVLAHGFGTDQSAWQLVYPSFTPYYRVILYDLVCAGSVN 60

Query: 61  PDFFDFSRYTTLDAFVDDLISILDSLHVHRCAFVGHSVSAMVGILASIRRPDLFSKLVLI 120
           PD FDFSRYTTLDAFVDDLI ILDSL V RCAFVGHSVSAMVGILASIRRP+LFSKLVLI
Sbjct: 61  PDHFDFSRYTTLDAFVDDLIFILDSLEVQRCAFVGHSVSAMVGILASIRRPELFSKLVLI 120

Query: 121 GASPRFLNDGDYHGGFEQSEIDRVFAAMKANYQAWVNGFAPLAVGADVPAAVQEFSRTLF 180
           GASPRFLNDGDYHGGFEQSEIDRVFAAMKANYQAWVNGFAPLAVGADVPAAVQEFSRTLF
Sbjct: 121 GASPRFLNDGDYHGGFEQSEIDRVFAAMKANYQAWVNGFAPLAVGADVPAAVQEFSRTLF 180

Query: 181 NMRPDISLFVSKVIFSSDLRGVLGLVKVPCCIIQTAQDVSVPASVAIYLRDHLGGRNTVE 240
           NMRPDISLFVSKVIFSSDLRGVLGLVKVPCCI+QTAQDVSVPASVAIYLRDHLGGRNTVE
Sbjct: 181 NMRPDISLFVSKVIFSSDLRGVLGLVKVPCCILQTAQDVSVPASVAIYLRDHLGGRNTVE 240

Query: 241 MLDTEGHLPHLSAPQLLVRKLRRALAR 268
           MLDTEGHLPHLSAPQLLVRKLRRAL+R
Sbjct: 241 MLDTEGHLPHLSAPQLLVRKLRRALSR 267

BLAST of Lag0004220 vs. ExPASy Swiss-Prot
Match: Q9SQR3 (Strigolactone esterase D14 OS=Arabidopsis thaliana OX=3702 GN=D14 PE=1 SV=1)

HSP 1 Score: 422.5 bits (1085), Expect = 3.4e-117
Identity = 204/267 (76.40%), Postives = 236/267 (88.39%), Query Frame = 0

Query: 1   MVNNALLEALNVRVLGTGDRFLVLAHGFGTDQSAWQLVYPSFTPYYRVILYDLVCAGSVN 60
           M  + +LEALNVRV+GTGDR L LAHGFGTDQSAW L+ P FT  YRV+LYDLVCAGSVN
Sbjct: 1   MSQHNILEALNVRVVGTGDRILFLAHGFGTDQSAWHLILPYFTQNYRVVLYDLVCAGSVN 60

Query: 61  PDFFDFSRYTTLDAFVDDLISILDSLHVHRCAFVGHSVSAMVGILASIRRPDLFSKLVLI 120
           PD+FDF+RYTTLD +VDDL++I+DSL +  CA+VGHSVSAM+GI+ASIRRP+LFSKL+LI
Sbjct: 61  PDYFDFNRYTTLDPYVDDLLNIVDSLGIQNCAYVGHSVSAMIGIIASIRRPELFSKLILI 120

Query: 121 GASPRFLNDGDYHGGFEQSEIDRVFAAMKANYQAWVNGFAPLAVGADVPAAVQEFSRTLF 180
           G SPRFLND DYHGGFE+ EI++VF+AM+ANY+AWV+GFAPLAVGADVPAAV+EFSRTLF
Sbjct: 121 GFSPRFLNDEDYHGGFEEGEIEKVFSAMEANYEAWVHGFAPLAVGADVPAAVREFSRTLF 180

Query: 181 NMRPDISLFVSKVIFSSDLRGVLGLVKVPCCIIQTAQDVSVPASVAIYLRDHLGGRNTVE 240
           NMRPDISLFVS+ +F+SDLRGVLGLV+VP C+IQTA+DVSVPASVA YLR HLGG  TVE
Sbjct: 181 NMRPDISLFVSRTVFNSDLRGVLGLVRVPTCVIQTAKDVSVPASVAEYLRSHLGGDTTVE 240

Query: 241 MLDTEGHLPHLSAPQLLVRKLRRALAR 268
            L TEGHLP LSAP  L + LRRAL R
Sbjct: 241 TLKTEGHLPQLSAPAQLAQFLRRALPR 267

BLAST of Lag0004220 vs. ExPASy Swiss-Prot
Match: A0A109QYD3 (Strigolactone esterase RMS3 OS=Pisum sativum OX=3888 GN=RMS3 PE=1 SV=1)

HSP 1 Score: 421.8 bits (1083), Expect = 5.9e-117
Identity = 200/263 (76.05%), Postives = 237/263 (90.11%), Query Frame = 0

Query: 6   LLEALNVRVLGTGDRFLVLAHGFGTDQSAWQLVYPSFTPYYRVILYDLVCAGSVNPDFFD 65
           +L+A NVRV G+GD++LV AHGFGTDQSAWQ V P FT  Y+VILYDLVCAGSVNPD FD
Sbjct: 5   ILDAFNVRVEGSGDKYLVFAHGFGTDQSAWQRVLPYFTRSYKVILYDLVCAGSVNPDHFD 64

Query: 66  FSRYTTLDAFVDDLISILDSLHVHRCAFVGHSVSAMVGILASIRRPDLFSKLVLIGASPR 125
           F RYTTLDA+VDDL++ILDSLHV RCA+VGHS+SAM G+LASIRRP+LFSKL+LIGASPR
Sbjct: 65  FRRYTTLDAYVDDLLNILDSLHVTRCAYVGHSISAMTGMLASIRRPELFSKLILIGASPR 124

Query: 126 FLNDGD-YHGGFEQSEIDRVFAAMKANYQAWVNGFAPLAVGADVPAAVQEFSRTLFNMRP 185
           FLNDG+ YHGGFEQ EI+ VF+AM+ANY+AWVNGFAPLAVGADVP AV+EFSRTLFNMRP
Sbjct: 125 FLNDGENYHGGFEQGEIEHVFSAMEANYEAWVNGFAPLAVGADVPTAVREFSRTLFNMRP 184

Query: 186 DISLFVSKVIFSSDLRGVLGLVKVPCCIIQTAQDVSVPASVAIYLRDHLGGRNTVEMLDT 245
           DISLFVS+ +F+SDLRG+LGLV VPCCI+QTA+D+SVPASVA Y+++H+GG++TV+ LDT
Sbjct: 185 DISLFVSRTVFNSDLRGILGLVNVPCCIMQTARDMSVPASVATYMKEHIGGKSTVQWLDT 244

Query: 246 EGHLPHLSAPQLLVRKLRRALAR 268
           EGHLPHLSAP  L  +L  AL++
Sbjct: 245 EGHLPHLSAPSYLAHQLEIALSQ 267

BLAST of Lag0004220 vs. ExPASy Swiss-Prot
Match: J9U5U9 (Probable strigolactone esterase DAD2 OS=Petunia hybrida OX=4102 GN=DAD2 PE=1 SV=1)

HSP 1 Score: 420.2 bits (1079), Expect = 1.7e-116
Identity = 202/265 (76.23%), Postives = 236/265 (89.06%), Query Frame = 0

Query: 2   VNNALLEALNVRVLGTGDRFLVLAHGFGTDQSAWQLVYPSFTPYYRVILYDLVCAGSVNP 61
           +   LL+ALNVRV+G+G+R LVLAHGFGTDQSAW  + P F   YRV+LYDLVCAGSVNP
Sbjct: 1   MGQTLLDALNVRVVGSGERVLVLAHGFGTDQSAWNRILPFFLRDYRVVLYDLVCAGSVNP 60

Query: 62  DFFDFSRYTTLDAFVDDLISILDSLHVHRCAFVGHSVSAMVGILASIRRPDLFSKLVLIG 121
           DFFDF RYTTLD +VDDL+ ILD+L +  CA+VGHSVSAM+GILASIRRP+LFSKL+LIG
Sbjct: 61  DFFDFRRYTTLDPYVDDLLHILDALGIDCCAYVGHSVSAMIGILASIRRPELFSKLILIG 120

Query: 122 ASPRFLNDGDYHGGFEQSEIDRVFAAMKANYQAWVNGFAPLAVGADVPAAVQEFSRTLFN 181
           ASPRFLND DYHGGFEQ EI++VF+AM+ANY+AWVNGFAPLAVGADVPAAV+EFSRTLFN
Sbjct: 121 ASPRFLNDEDYHGGFEQGEIEKVFSAMEANYEAWVNGFAPLAVGADVPAAVREFSRTLFN 180

Query: 182 MRPDISLFVSKVIFSSDLRGVLGLVKVPCCIIQTAQDVSVPASVAIYLRDHLGGRNTVEM 241
           MRPDI+LFVS+ +F+SD+RGVLGLVKVPC I QTA+D SVPASVA YL++HLGG+NTV  
Sbjct: 181 MRPDITLFVSRTVFNSDMRGVLGLVKVPCHIFQTARDHSVPASVATYLKNHLGGKNTVHW 240

Query: 242 LDTEGHLPHLSAPQLLVRKLRRALA 267
           L+ EGHLPHLSAP LL ++LRRAL+
Sbjct: 241 LNIEGHLPHLSAPTLLAQELRRALS 265

BLAST of Lag0004220 vs. ExPASy Swiss-Prot
Match: Q10QA5 (Strigolactone esterase D14 OS=Oryza sativa subsp. japonica OX=39947 GN=D14 PE=1 SV=1)

HSP 1 Score: 404.1 bits (1037), Expect = 1.3e-111
Identity = 196/262 (74.81%), Postives = 223/262 (85.11%), Query Frame = 0

Query: 6   LLEALNVRVLGTGDRFLVLAHGFGTDQSAWQLVYPSFTPYYRVILYDLVCAGSVNPDFFD 65
           LL+ LNVRV+G+G+R +VL+HGFGTDQSAW  V P  T  +RV+LYDLVCAGSVNPD FD
Sbjct: 56  LLQILNVRVVGSGERVVVLSHGFGTDQSAWSRVLPYLTRDHRVVLYDLVCAGSVNPDHFD 115

Query: 66  FSRYTTLDAFVDDLISILDSLHVHRCAFVGHSVSAMVGILASIRRPDLFSKLVLIGASPR 125
           F RY  LDA+VDDL++ILD+L + RCAFVGHSVSAM+GILASIRRPDLF+KLVLIGASPR
Sbjct: 116 FRRYDNLDAYVDDLLAILDALRIPRCAFVGHSVSAMIGILASIRRPDLFAKLVLIGASPR 175

Query: 126 FLNDGDYHGGFEQSEIDRVFAAMKANYQAWVNGFAPLAVGADVPAAVQEFSRTLFNMRPD 185
           FLND DYHGGFE  EI +VF AM ANY AW  G+APLAVGADVPAAVQEFSRTLFNMRPD
Sbjct: 176 FLNDSDYHGGFELEEIQQVFDAMGANYSAWATGYAPLAVGADVPAAVQEFSRTLFNMRPD 235

Query: 186 ISLFVSKVIFSSDLRGVLGLVKVPCCIIQTAQDVSVPASVAIYLRDHLGGRNTVEMLDTE 245
           ISL V + +F +DLRGVLG+V+ PC ++QT +DVSVPASVA YL+ HLGGR TVE L TE
Sbjct: 236 ISLHVCQTVFKTDLRGVLGMVRAPCVVVQTTRDVSVPASVAAYLKAHLGGRTTVEFLQTE 295

Query: 246 GHLPHLSAPQLLVRKLRRALAR 268
           GHLPHLSAP LL + LRRALAR
Sbjct: 296 GHLPHLSAPSLLAQVLRRALAR 317

BLAST of Lag0004220 vs. ExPASy Swiss-Prot
Match: Q10J20 (Probable esterase D14L OS=Oryza sativa subsp. japonica OX=39947 GN=D14L PE=1 SV=1)

HSP 1 Score: 307.0 bits (785), Expect = 2.1e-82
Identity = 149/258 (57.75%), Postives = 197/258 (76.36%), Query Frame = 0

Query: 8   EALNVRVLGTGDR-FLVLAHGFGTDQSAWQLVYPSFTPYYRVILYDLVCAGSVNPDFFDF 67
           EA N+RV+G G R  +VLAHGFGTDQS W+ + P     YRV+L+D + AG  NPD+FDF
Sbjct: 6   EAHNLRVVGEGKRGVIVLAHGFGTDQSVWKHLVPHLVADYRVVLFDTMGAGPTNPDYFDF 65

Query: 68  SRYTTLDAFVDDLISILDSLHVHRCAFVGHSVSAMVGILASIRRPDLFSKLVLIGASPRF 127
           SRY TL+ +  DL++IL  L V  C +VGHSVSA++G +ASI RPDLFSKLVL+ ASPR+
Sbjct: 66  SRYATLEGYALDLLAILQELRVASCIYVGHSVSAVIGAIASISRPDLFSKLVLLSASPRY 125

Query: 128 LNDGDYHGGFEQSEIDRVFAAMKANYQAWVNGFAPLAVGADVPA-AVQEFSRTLFNMRPD 187
           LND DY+GGFEQ ++D +F AM +NY+AW +GFAPL VG D+ + AVQEFSRTLFN+RPD
Sbjct: 126 LNDVDYYGGFEQEDLDELFEAMGSNYKAWCSGFAPLCVGGDMESVAVQEFSRTLFNIRPD 185

Query: 188 ISLFVSKVIFSSDLRGVLGLVKVPCCIIQTAQDVSVPASVAIYLRDHLGGRNTVEMLDTE 247
           I+L V++ IF SD+R +L LV VPC I+Q+ +D++VP  V+ YL  HLGG + VE++ +E
Sbjct: 186 IALSVAQTIFQSDVRSLLPLVTVPCHIVQSTKDLAVPVVVSEYLHKHLGGDSIVEVMPSE 245

Query: 248 GHLPHLSAPQLLVRKLRR 264
           GHLP LS+P +++  L R
Sbjct: 246 GHLPQLSSPDIVIPVLLR 263

BLAST of Lag0004220 vs. ExPASy TrEMBL
Match: A0A5A7V5G5 (Strigolactone esterase D14 OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold501G00820 PE=4 SV=1)

HSP 1 Score: 528.1 bits (1359), Expect = 2.1e-146
Identity = 261/267 (97.75%), Postives = 266/267 (99.63%), Query Frame = 0

Query: 1   MVNNALLEALNVRVLGTGDRFLVLAHGFGTDQSAWQLVYPSFTPYYRVILYDLVCAGSVN 60
           MVNNALLEALNVRVLGTGDRFLVLAHGFGTDQSAWQLVYPSFTPYYRVILYDLVCAGSVN
Sbjct: 1   MVNNALLEALNVRVLGTGDRFLVLAHGFGTDQSAWQLVYPSFTPYYRVILYDLVCAGSVN 60

Query: 61  PDFFDFSRYTTLDAFVDDLISILDSLHVHRCAFVGHSVSAMVGILASIRRPDLFSKLVLI 120
           PDFFDFSRYTTLDAFVDDLISILDSLHVHRCAFVGHSVSAMVGILASIRRP+LFSKL+LI
Sbjct: 61  PDFFDFSRYTTLDAFVDDLISILDSLHVHRCAFVGHSVSAMVGILASIRRPELFSKLILI 120

Query: 121 GASPRFLNDGDYHGGFEQSEIDRVFAAMKANYQAWVNGFAPLAVGADVPAAVQEFSRTLF 180
           GASPRFLNDGDYHGGFEQSEIDRVFAAMKANYQ+WVNGFAPLAVGADVPAAVQEFSRTLF
Sbjct: 121 GASPRFLNDGDYHGGFEQSEIDRVFAAMKANYQSWVNGFAPLAVGADVPAAVQEFSRTLF 180

Query: 181 NMRPDISLFVSKVIFSSDLRGVLGLVKVPCCIIQTAQDVSVPASVAIYLRDHLGGRNTVE 240
           NMRPDISLFVSKVIFSSDLRGVLGLVKVPCCIIQTAQDVSVP SVAIYLRDHLGGRNT+E
Sbjct: 181 NMRPDISLFVSKVIFSSDLRGVLGLVKVPCCIIQTAQDVSVPTSVAIYLRDHLGGRNTIE 240

Query: 241 MLDTEGHLPHLSAPQLLVRKLRRALAR 268
           MLDTEGHLPHLSAPQLLVRKLRRAL+R
Sbjct: 241 MLDTEGHLPHLSAPQLLVRKLRRALSR 267

BLAST of Lag0004220 vs. ExPASy TrEMBL
Match: A0A1S3BA45 (strigolactone esterase D14 OS=Cucumis melo OX=3656 GN=LOC103487842 PE=4 SV=1)

HSP 1 Score: 528.1 bits (1359), Expect = 2.1e-146
Identity = 261/267 (97.75%), Postives = 266/267 (99.63%), Query Frame = 0

Query: 1   MVNNALLEALNVRVLGTGDRFLVLAHGFGTDQSAWQLVYPSFTPYYRVILYDLVCAGSVN 60
           MVNNALLEALNVRVLGTGDRFLVLAHGFGTDQSAWQLVYPSFTPYYRVILYDLVCAGSVN
Sbjct: 1   MVNNALLEALNVRVLGTGDRFLVLAHGFGTDQSAWQLVYPSFTPYYRVILYDLVCAGSVN 60

Query: 61  PDFFDFSRYTTLDAFVDDLISILDSLHVHRCAFVGHSVSAMVGILASIRRPDLFSKLVLI 120
           PDFFDFSRYTTLDAFVDDLISILDSLHVHRCAFVGHSVSAMVGILASIRRP+LFSKL+LI
Sbjct: 61  PDFFDFSRYTTLDAFVDDLISILDSLHVHRCAFVGHSVSAMVGILASIRRPELFSKLILI 120

Query: 121 GASPRFLNDGDYHGGFEQSEIDRVFAAMKANYQAWVNGFAPLAVGADVPAAVQEFSRTLF 180
           GASPRFLNDGDYHGGFEQSEIDRVFAAMKANYQ+WVNGFAPLAVGADVPAAVQEFSRTLF
Sbjct: 121 GASPRFLNDGDYHGGFEQSEIDRVFAAMKANYQSWVNGFAPLAVGADVPAAVQEFSRTLF 180

Query: 181 NMRPDISLFVSKVIFSSDLRGVLGLVKVPCCIIQTAQDVSVPASVAIYLRDHLGGRNTVE 240
           NMRPDISLFVSKVIFSSDLRGVLGLVKVPCCIIQTAQDVSVP SVAIYLRDHLGGRNT+E
Sbjct: 181 NMRPDISLFVSKVIFSSDLRGVLGLVKVPCCIIQTAQDVSVPTSVAIYLRDHLGGRNTIE 240

Query: 241 MLDTEGHLPHLSAPQLLVRKLRRALAR 268
           MLDTEGHLPHLSAPQLLVRKLRRAL+R
Sbjct: 241 MLDTEGHLPHLSAPQLLVRKLRRALSR 267

BLAST of Lag0004220 vs. ExPASy TrEMBL
Match: A0A0A0LNU2 (Sigma factor sigb regulation protein rsbq OS=Cucumis sativus OX=3659 GN=Csa_2G354780 PE=4 SV=1)

HSP 1 Score: 526.9 bits (1356), Expect = 4.8e-146
Identity = 260/267 (97.38%), Postives = 266/267 (99.63%), Query Frame = 0

Query: 1   MVNNALLEALNVRVLGTGDRFLVLAHGFGTDQSAWQLVYPSFTPYYRVILYDLVCAGSVN 60
           MVNNALLEALNVRVLGTGDRFLVLAHGFGTDQSAWQLVYPSFTPYYRVILYDLVCAGSVN
Sbjct: 1   MVNNALLEALNVRVLGTGDRFLVLAHGFGTDQSAWQLVYPSFTPYYRVILYDLVCAGSVN 60

Query: 61  PDFFDFSRYTTLDAFVDDLISILDSLHVHRCAFVGHSVSAMVGILASIRRPDLFSKLVLI 120
           PDFFDFSRYTTLDAFVDDLISILDSLHVHRCAFVGHSVSAMVGILASIRRP+LFSKL+LI
Sbjct: 61  PDFFDFSRYTTLDAFVDDLISILDSLHVHRCAFVGHSVSAMVGILASIRRPELFSKLILI 120

Query: 121 GASPRFLNDGDYHGGFEQSEIDRVFAAMKANYQAWVNGFAPLAVGADVPAAVQEFSRTLF 180
           GASPRFLNDGDYHGGFEQ+EIDRVFAAMKANYQ+WVNGFAPLAVGADVPAAVQEFSRTLF
Sbjct: 121 GASPRFLNDGDYHGGFEQNEIDRVFAAMKANYQSWVNGFAPLAVGADVPAAVQEFSRTLF 180

Query: 181 NMRPDISLFVSKVIFSSDLRGVLGLVKVPCCIIQTAQDVSVPASVAIYLRDHLGGRNTVE 240
           NMRPDISLFVSKVIFSSDLRGVLGLVKVPCCIIQTAQDVSVP SVAIYLRDHLGGRNT+E
Sbjct: 181 NMRPDISLFVSKVIFSSDLRGVLGLVKVPCCIIQTAQDVSVPTSVAIYLRDHLGGRNTIE 240

Query: 241 MLDTEGHLPHLSAPQLLVRKLRRALAR 268
           MLDTEGHLPHLSAPQLLVRKLRRAL+R
Sbjct: 241 MLDTEGHLPHLSAPQLLVRKLRRALSR 267

BLAST of Lag0004220 vs. ExPASy TrEMBL
Match: A0A6J1K9V8 (strigolactone esterase RMS3 OS=Cucurbita maxima OX=3661 GN=LOC111491982 PE=4 SV=1)

HSP 1 Score: 514.2 bits (1323), Expect = 3.2e-142
Identity = 257/267 (96.25%), Postives = 261/267 (97.75%), Query Frame = 0

Query: 1   MVNNALLEALNVRVLGTGDRFLVLAHGFGTDQSAWQLVYPSFTPYYRVILYDLVCAGSVN 60
           MVNNALL+A NVRVLGTGDR LVLAHGFGTDQSAWQLVYPSFTPYYRVILYDLVCAGSVN
Sbjct: 1   MVNNALLDAFNVRVLGTGDRVLVLAHGFGTDQSAWQLVYPSFTPYYRVILYDLVCAGSVN 60

Query: 61  PDFFDFSRYTTLDAFVDDLISILDSLHVHRCAFVGHSVSAMVGILASIRRPDLFSKLVLI 120
           PD FDFSRYTTLDAFVDDLI ILDSL V RCAFVGHSVSAMVGILASIRRP+LFSKLVLI
Sbjct: 61  PDHFDFSRYTTLDAFVDDLIFILDSLEVQRCAFVGHSVSAMVGILASIRRPELFSKLVLI 120

Query: 121 GASPRFLNDGDYHGGFEQSEIDRVFAAMKANYQAWVNGFAPLAVGADVPAAVQEFSRTLF 180
           GASPRFLNDGDYHGGFEQSEIDRVFAAMKANYQAWVNGFAPLAVGADVPAAVQEFSRTLF
Sbjct: 121 GASPRFLNDGDYHGGFEQSEIDRVFAAMKANYQAWVNGFAPLAVGADVPAAVQEFSRTLF 180

Query: 181 NMRPDISLFVSKVIFSSDLRGVLGLVKVPCCIIQTAQDVSVPASVAIYLRDHLGGRNTVE 240
           NMRPDISLFVSKVIFSSDLRGVLGLVKVPCCI+QTAQDVSVPASVAIYLRDHLGGRNTVE
Sbjct: 181 NMRPDISLFVSKVIFSSDLRGVLGLVKVPCCILQTAQDVSVPASVAIYLRDHLGGRNTVE 240

Query: 241 MLDTEGHLPHLSAPQLLVRKLRRALAR 268
           MLDTEGHLPHLSAPQLLVRKLRRAL+R
Sbjct: 241 MLDTEGHLPHLSAPQLLVRKLRRALSR 267

BLAST of Lag0004220 vs. ExPASy TrEMBL
Match: A0A6J1HEB7 (strigolactone esterase RMS3 OS=Cucurbita moschata OX=3662 GN=LOC111462766 PE=4 SV=1)

HSP 1 Score: 513.5 bits (1321), Expect = 5.5e-142
Identity = 257/267 (96.25%), Postives = 261/267 (97.75%), Query Frame = 0

Query: 1   MVNNALLEALNVRVLGTGDRFLVLAHGFGTDQSAWQLVYPSFTPYYRVILYDLVCAGSVN 60
           MVNNA L+ALNVRVLGTGDR LVLAHGFGTDQSAWQLVYPSFTPYYRVILYDLVCAGSVN
Sbjct: 1   MVNNARLDALNVRVLGTGDRVLVLAHGFGTDQSAWQLVYPSFTPYYRVILYDLVCAGSVN 60

Query: 61  PDFFDFSRYTTLDAFVDDLISILDSLHVHRCAFVGHSVSAMVGILASIRRPDLFSKLVLI 120
           PD FDFSRYTTLDAFVDDLI ILDSL V RCAFVGHSVSAMVGILASIRRP+LF+KLVLI
Sbjct: 61  PDHFDFSRYTTLDAFVDDLIFILDSLEVQRCAFVGHSVSAMVGILASIRRPELFTKLVLI 120

Query: 121 GASPRFLNDGDYHGGFEQSEIDRVFAAMKANYQAWVNGFAPLAVGADVPAAVQEFSRTLF 180
           GASPRFLNDGDYHGGFEQSEIDRVFAAMKANYQAWVNGFAPLAVGADVPAAVQEFSRTLF
Sbjct: 121 GASPRFLNDGDYHGGFEQSEIDRVFAAMKANYQAWVNGFAPLAVGADVPAAVQEFSRTLF 180

Query: 181 NMRPDISLFVSKVIFSSDLRGVLGLVKVPCCIIQTAQDVSVPASVAIYLRDHLGGRNTVE 240
           NMRPDISLFVSKVIFSSDLRGVLGLVKVPCCI+QTAQDVSVPASVAIYLRDHLGGRNTVE
Sbjct: 181 NMRPDISLFVSKVIFSSDLRGVLGLVKVPCCILQTAQDVSVPASVAIYLRDHLGGRNTVE 240

Query: 241 MLDTEGHLPHLSAPQLLVRKLRRALAR 268
           MLDTEGHLPHLSAPQLLVRKLRRALAR
Sbjct: 241 MLDTEGHLPHLSAPQLLVRKLRRALAR 267

BLAST of Lag0004220 vs. TAIR 10
Match: AT3G03990.1 (alpha/beta-Hydrolases superfamily protein )

HSP 1 Score: 422.5 bits (1085), Expect = 2.4e-118
Identity = 204/267 (76.40%), Postives = 236/267 (88.39%), Query Frame = 0

Query: 1   MVNNALLEALNVRVLGTGDRFLVLAHGFGTDQSAWQLVYPSFTPYYRVILYDLVCAGSVN 60
           M  + +LEALNVRV+GTGDR L LAHGFGTDQSAW L+ P FT  YRV+LYDLVCAGSVN
Sbjct: 1   MSQHNILEALNVRVVGTGDRILFLAHGFGTDQSAWHLILPYFTQNYRVVLYDLVCAGSVN 60

Query: 61  PDFFDFSRYTTLDAFVDDLISILDSLHVHRCAFVGHSVSAMVGILASIRRPDLFSKLVLI 120
           PD+FDF+RYTTLD +VDDL++I+DSL +  CA+VGHSVSAM+GI+ASIRRP+LFSKL+LI
Sbjct: 61  PDYFDFNRYTTLDPYVDDLLNIVDSLGIQNCAYVGHSVSAMIGIIASIRRPELFSKLILI 120

Query: 121 GASPRFLNDGDYHGGFEQSEIDRVFAAMKANYQAWVNGFAPLAVGADVPAAVQEFSRTLF 180
           G SPRFLND DYHGGFE+ EI++VF+AM+ANY+AWV+GFAPLAVGADVPAAV+EFSRTLF
Sbjct: 121 GFSPRFLNDEDYHGGFEEGEIEKVFSAMEANYEAWVHGFAPLAVGADVPAAVREFSRTLF 180

Query: 181 NMRPDISLFVSKVIFSSDLRGVLGLVKVPCCIIQTAQDVSVPASVAIYLRDHLGGRNTVE 240
           NMRPDISLFVS+ +F+SDLRGVLGLV+VP C+IQTA+DVSVPASVA YLR HLGG  TVE
Sbjct: 181 NMRPDISLFVSRTVFNSDLRGVLGLVRVPTCVIQTAKDVSVPASVAEYLRSHLGGDTTVE 240

Query: 241 MLDTEGHLPHLSAPQLLVRKLRRALAR 268
            L TEGHLP LSAP  L + LRRAL R
Sbjct: 241 TLKTEGHLPQLSAPAQLAQFLRRALPR 267

BLAST of Lag0004220 vs. TAIR 10
Match: AT4G37470.1 (alpha/beta-Hydrolases superfamily protein )

HSP 1 Score: 300.8 bits (769), Expect = 1.1e-81
Identity = 143/264 (54.17%), Postives = 200/264 (75.76%), Query Frame = 0

Query: 8   EALNVRVLGTGDRFLVLAHGFGTDQSAWQLVYPSFTPYYRVILYDLVCAGSVNPDFFDFS 67
           EA NV+V+G+G+  +VL HGFGTDQS W+ + P     YRV+LYD + AG+ NPD+FDF 
Sbjct: 6   EAHNVKVIGSGEATIVLGHGFGTDQSVWKHLVPHLVDDYRVVLYDNMGAGTTNPDYFDFD 65

Query: 68  RYTTLDAFVDDLISILDSLHVHRCAFVGHSVSAMVGILASIRRPDLFSKLVLIGASPRFL 127
           RY+ L+ +  DLI+IL+ L +  C FVGHSVSAM+G+LAS+ RPDLFSK+V+I ASPR++
Sbjct: 66  RYSNLEGYSFDLIAILEDLKIESCIFVGHSVSAMIGVLASLNRPDLFSKIVMISASPRYV 125

Query: 128 NDGDYHGGFEQSEIDRVFAAMKANYQAWVNGFAPLAVGADVPA-AVQEFSRTLFNMRPDI 187
           ND DY GGFEQ +++++F A+++NY+AW  GFAPLAVG D+ + AVQEFSRTLFNMRPDI
Sbjct: 126 NDVDYQGGFEQEDLNQLFEAIRSNYKAWCLGFAPLAVGGDMDSIAVQEFSRTLFNMRPDI 185

Query: 188 SLFVSKVIFSSDLRGVLGLVKVPCCIIQTAQDVSVPASVAIYLRDHLGGRNTVEMLDTEG 247
           +L V + IF SD+R +L  V VPC I+Q+ +D++VP  V+ YL  +LG  + VE++ ++G
Sbjct: 186 ALSVGQTIFQSDMRQILPFVTVPCHILQSVKDLAVPVVVSEYLHANLGCESVVEVIPSDG 245

Query: 248 HLPHLSAPQ----LLVRKLRRALA 267
           HLP LS+P     +++R +R  +A
Sbjct: 246 HLPQLSSPDSVIPVILRHIRNDIA 269

BLAST of Lag0004220 vs. TAIR 10
Match: AT3G24420.1 (alpha/beta-Hydrolases superfamily protein )

HSP 1 Score: 231.5 bits (589), Expect = 8.0e-61
Identity = 108/264 (40.91%), Postives = 177/264 (67.05%), Query Frame = 0

Query: 4   NALLEALNVRVLGTGDRFLVLAHGFGTDQSAWQLVYPSFTPYYRVILYDLVCAGSV-NPD 63
           + L  A+N +++G+G+R +VLAHGFG DQS W  + P  +  ++V+++D + +G++ +  
Sbjct: 8   SGLASAMNAKIIGSGERSMVLAHGFGGDQSVWDKIIPVLSQSFKVLVFDWLFSGAIKDQT 67

Query: 64  FFDFSRYTTLDAFVDDLISILDSLHVHRCAFVGHSVSAMVGILASIRRPDLFSKLVLIGA 123
            +D S+Y +LD F DDLI++++ L      FVGHS+S ++G  ASI+RPDLF+ L+LI A
Sbjct: 68  LYDPSKYNSLDVFSDDLIALMEELKFGPVVFVGHSMSGVIGCAASIKRPDLFTNLLLIAA 127

Query: 124 SPRFLNDGDYHGGFEQSEIDRVFAAMKANYQAWVNGFAPLAVGADVPAAVQEFSRTLFNM 183
           SPR++N  DY GGFE  +ID +  ++ +NY+AW   F+   V +    +VQ F ++L  M
Sbjct: 128 SPRYINSEDYKGGFESKDIDTIITSIGSNYEAWAVDFSSFVVDSRDSLSVQRFEKSLKKM 187

Query: 184 RPDISLFVSKVIFSSDLRGVLGLVKVPCCIIQTAQDVSVPASVAIYLRDHLGGRNTVEML 243
           +P+ +L ++K++F SD R +LG V VPC +IQ   DV VP SVA ++++ + G++TVE++
Sbjct: 188 KPETALALAKIVFGSDEREILGQVSVPCHVIQPGNDVVVPVSVAYFMQEKIKGKSTVEII 247

Query: 244 -DTEGHLPHLSAPQLLVRKLRRAL 266
            D  GH P +++   L+  +RR L
Sbjct: 248 EDAIGHFPQMTSHLELLGVMRRLL 271

BLAST of Lag0004220 vs. TAIR 10
Match: AT5G19850.1 (alpha/beta-Hydrolases superfamily protein )

HSP 1 Score: 53.9 bits (128), Expect = 2.3e-07
Identity = 33/123 (26.83%), Postives = 57/123 (46.34%), Query Frame = 0

Query: 9   ALNVRVLGTGDRFLVLAHGFGTDQSAWQLVYPSFTPYYRVILYDLVCAG---SVNPDFFD 68
           ++  +  GT    LVL HGFG +   W+   P     +RV   DL+  G     NP  F 
Sbjct: 84  SIRYQCAGTSGPALVLVHGFGANSDHWRKNTPILGKTHRVYSIDLIGYGYSDKPNPREFG 143

Query: 69  FSRYTTLDAFVDDLISILDSLHVHRCAFVGHSVSAMVGILASIRRPDLFSKLVLIGASPR 128
              + T + + + L      +      F+ +S+  +VG+ A++ +P++   L+LI  S R
Sbjct: 144 GEPFYTFETWGEQLNDFCLDVVKDEAFFICNSIGGLVGLQAAVSKPEICRGLMLINISLR 203

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
XP_008444562.14.4e-14697.75PREDICTED: strigolactone esterase D14 [Cucumis melo] >KAA0060949.1 strigolactone... [more]
XP_004142890.19.9e-14697.38strigolactone esterase RMS3 [Cucumis sativus] >KGN62452.1 hypothetical protein C... [more]
XP_038886523.18.3e-14597.38strigolactone esterase D14 [Benincasa hispida][more]
XP_023546980.11.7e-14296.63strigolactone esterase RMS3 [Cucurbita pepo subsp. pepo][more]
XP_022996864.16.6e-14296.25strigolactone esterase RMS3 [Cucurbita maxima][more]
Match NameE-valueIdentityDescription
Q9SQR33.4e-11776.40Strigolactone esterase D14 OS=Arabidopsis thaliana OX=3702 GN=D14 PE=1 SV=1[more]
A0A109QYD35.9e-11776.05Strigolactone esterase RMS3 OS=Pisum sativum OX=3888 GN=RMS3 PE=1 SV=1[more]
J9U5U91.7e-11676.23Probable strigolactone esterase DAD2 OS=Petunia hybrida OX=4102 GN=DAD2 PE=1 SV=... [more]
Q10QA51.3e-11174.81Strigolactone esterase D14 OS=Oryza sativa subsp. japonica OX=39947 GN=D14 PE=1 ... [more]
Q10J202.1e-8257.75Probable esterase D14L OS=Oryza sativa subsp. japonica OX=39947 GN=D14L PE=1 SV=... [more]
Match NameE-valueIdentityDescription
A0A5A7V5G52.1e-14697.75Strigolactone esterase D14 OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaff... [more]
A0A1S3BA452.1e-14697.75strigolactone esterase D14 OS=Cucumis melo OX=3656 GN=LOC103487842 PE=4 SV=1[more]
A0A0A0LNU24.8e-14697.38Sigma factor sigb regulation protein rsbq OS=Cucumis sativus OX=3659 GN=Csa_2G35... [more]
A0A6J1K9V83.2e-14296.25strigolactone esterase RMS3 OS=Cucurbita maxima OX=3661 GN=LOC111491982 PE=4 SV=... [more]
A0A6J1HEB75.5e-14296.25strigolactone esterase RMS3 OS=Cucurbita moschata OX=3662 GN=LOC111462766 PE=4 S... [more]
Match NameE-valueIdentityDescription
AT3G03990.12.4e-11876.40alpha/beta-Hydrolases superfamily protein [more]
AT4G37470.11.1e-8154.17alpha/beta-Hydrolases superfamily protein [more]
AT3G24420.18.0e-6140.91alpha/beta-Hydrolases superfamily protein [more]
AT5G19850.12.3e-0726.83alpha/beta-Hydrolases superfamily protein [more]
InterPro
Analysis Name: InterPro Annotations of Sponge gourd (AG-4) v1
Date Performed: 2022-08-01
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR029058Alpha/Beta hydrolase foldGENE3D3.40.50.1820alpha/beta hydrolasecoord: 2..266
e-value: 5.1E-73
score: 248.0
IPR029058Alpha/Beta hydrolase foldSUPERFAMILY53474alpha/beta-Hydrolasescoord: 10..266
IPR000073Alpha/beta hydrolase fold-1PFAMPF12697Abhydrolase_6coord: 22..259
e-value: 3.1E-14
score: 54.1
NoneNo IPR availablePANTHERPTHR43039ESTERASE-RELATEDcoord: 3..266
IPR031143Strigolactone esterase D14 familyPANTHERPTHR43039:SF4STRIGOLACTONE ESTERASE D14coord: 3..266

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Lag0004220.1Lag0004220.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:1902348 cellular response to strigolactone
biological_process GO:0010223 secondary shoot formation
biological_process GO:1901601 strigolactone biosynthetic process
molecular_function GO:0016787 hydrolase activity