Lag0004204 (gene) Sponge gourd (AG‐4) v1

BLAST of Lag0004204 vs. NCBI nr
Match: XP_038884660.1 (U-box domain-containing protein 31-like [Benincasa hispida])

HSP 1 Score: 828.2 bits (2138), Expect = 3.5e-236
Identity = 431/452 (95.35%), Postives = 440/452 (97.35%), Query Frame = 0

Query: 1   MPMYQPSAWRDGDPKLVVGCGAQILDLETVVKDGILGGGGGGGLISGGGAEKLDLKEMIE 60
           MPMYQP+ WR+GDPKLVVGCGAQILDLETVVKDGIL  GGGGG+I GGG EKLDLK+MIE
Sbjct: 1   MPMYQPATWREGDPKLVVGCGAQILDLETVVKDGIL--GGGGGIICGGG-EKLDLKDMIE 60

Query: 61  ELESSIEVPSVFICPISLEPMQDPVTLCSGQTYERSNILKWFSLGHLTCPTTMQELWDDS 120
           ELESSIEVPSVFICPISLEPMQDPVTLCSGQTYERSNILKWFSLGHLTCPTTMQELWDDS
Sbjct: 61  ELESSIEVPSVFICPISLEPMQDPVTLCSGQTYERSNILKWFSLGHLTCPTTMQELWDDS 120

Query: 121 VTPNRTLHQLIHTWFSQKYLALKKRSEDVQGRVLELLDSLKKVKGQARVQSLKDLRHVVA 180
           VTPNRTLHQLIHTWFSQKYLALKKRSEDVQGRVLELLDSLKKVKGQARVQSLKDLRHVV 
Sbjct: 121 VTPNRTLHQLIHTWFSQKYLALKKRSEDVQGRVLELLDSLKKVKGQARVQSLKDLRHVVT 180

Query: 181 THSSAKKMVVDNGGVALISSLLGSFTSHAVGSEAVGILVNLDLDSELKKNLIQPTRMSLI 240
           THSSAKKMVVDNGGVALISSLLGSFTSHAVGSEAVGILVNLDLDSELKKNLIQPTR+SLI
Sbjct: 181 THSSAKKMVVDNGGVALISSLLGSFTSHAVGSEAVGILVNLDLDSELKKNLIQPTRISLI 240

Query: 241 ADILNEGSIETKINCTKFIKMLIEDDDLRTENVSSLSLLVGLLRMVKDQRHPNGVTAGLS 300
           ADILNEGSIETKINCTKFIKMLI  +DLRTENVSSLSLLVGLLRMVKDQRHPNGVTAGLS
Sbjct: 241 ADILNEGSIETKINCTKFIKMLIVGEDLRTENVSSLSLLVGLLRMVKDQRHPNGVTAGLS 300

Query: 301 LLRTLCSNESIRKQLVSIGAVAKLGGALSGLNADCLESALYILDVLSSLPEGRLALKDCE 360
           LLRTLCSNE IRKQLVSIGAVAKLGGALS LN DCLESALYILDVLSSLPEGRLALK+CE
Sbjct: 301 LLRTLCSNEPIRKQLVSIGAVAKLGGALSNLNTDCLESALYILDVLSSLPEGRLALKNCE 360

Query: 361 DTIRNLVKLLMKVSEICTQFALSILWAVFKLAPEECAVLAVEAGLAAKLLLVIQSGCNKE 420
           DTIRNLVKLLMKVSEICTQFALSILWAVFKLAPEEC+VLAVEAGLAAKLLLVIQSGCN E
Sbjct: 361 DTIRNLVKLLMKVSEICTQFALSILWAVFKLAPEECSVLAVEAGLAAKLLLVIQSGCNPE 420

Query: 421 LKQRSSELLKLCSLNYPDSIFISKCKLTRTIQ 453
           LKQRS+ELLKLCSLNYPDSIFISKCKLTRTIQ
Sbjct: 421 LKQRSAELLKLCSLNYPDSIFISKCKLTRTIQ 449

BLAST of Lag0004204 vs. NCBI nr
Match: XP_031736908.1 (U-box domain-containing protein 30 isoform X2 [Cucumis sativus])

HSP 1 Score: 815.5 bits (2105), Expect = 2.3e-232
Identity = 426/457 (93.22%), Postives = 437/457 (95.62%), Query Frame = 0

Query: 1   MPMYQP-SAWRDGDPKLVVGCGAQILDLETVVKDGILGGGG----GGGLISGGGAEKLDL 60
           MPMYQP + WR+GDPKLVVGCG QILDLETVVKDGILGGGG    GGG   GGG EKLDL
Sbjct: 1   MPMYQPATTWREGDPKLVVGCGTQILDLETVVKDGILGGGGGIICGGG--GGGGGEKLDL 60

Query: 61  KEMIEELESSIEVPSVFICPISLEPMQDPVTLCSGQTYERSNILKWFSLGHLTCPTTMQE 120
           KEMIEELESS+EVPSVFICPISLEPMQDPVTLCSGQTYERSNILKWFSLGHLTCPTTMQE
Sbjct: 61  KEMIEELESSVEVPSVFICPISLEPMQDPVTLCSGQTYERSNILKWFSLGHLTCPTTMQE 120

Query: 121 LWDDSVTPNRTLHQLIHTWFSQKYLALKKRSEDVQGRVLELLDSLKKVKGQARVQSLKDL 180
           LWDDSVTPNRTLHQLIHTWFSQKYLALKKRSEDVQGRVLELLDSLKKVKGQARVQSLKDL
Sbjct: 121 LWDDSVTPNRTLHQLIHTWFSQKYLALKKRSEDVQGRVLELLDSLKKVKGQARVQSLKDL 180

Query: 181 RHVVATHSSAKKMVVDNGGVALISSLLGSFTSHAVGSEAVGILVNLDLDSELKKNLIQPT 240
           RH+V THSSAKKMVVDNGGVALISSLLGSFTSHAVGSEAVGILVNLDL+SELKKNLIQPT
Sbjct: 181 RHIVITHSSAKKMVVDNGGVALISSLLGSFTSHAVGSEAVGILVNLDLNSELKKNLIQPT 240

Query: 241 RMSLIADILNEGSIETKINCTKFIKMLIEDDDLRTENVSSLSLLVGLLRMVKDQRHPNGV 300
           R+SLIADILNEGSIETKINCTKF KMLI  +DL+TE+VSSLSLLVGLLRMVKDQRHPNGV
Sbjct: 241 RISLIADILNEGSIETKINCTKFFKMLIVGEDLKTEDVSSLSLLVGLLRMVKDQRHPNGV 300

Query: 301 TAGLSLLRTLCSNESIRKQLVSIGAVAKLGGALSGLNADCLESALYILDVLSSLPEGRLA 360
           TAGLSLLRTLCSNE IRKQLVSIGAVAKLGG LS LN DCLESALYILDVLSSLPEGRLA
Sbjct: 301 TAGLSLLRTLCSNEPIRKQLVSIGAVAKLGGTLSNLNTDCLESALYILDVLSSLPEGRLA 360

Query: 361 LKDCEDTIRNLVKLLMKVSEICTQFALSILWAVFKLAPEECAVLAVEAGLAAKLLLVIQS 420
           LK+CEDTIRNLVKLLMKVSEICTQFALSILWAVFKLAPEECAVLAVEAGLAAKLLLVIQS
Sbjct: 361 LKNCEDTIRNLVKLLMKVSEICTQFALSILWAVFKLAPEECAVLAVEAGLAAKLLLVIQS 420

Query: 421 GCNKELKQRSSELLKLCSLNYPDSIFISKCKLTRTIQ 453
           GCN ELKQRS+ELLKLCSLNYPDSIFISKCKLTRTIQ
Sbjct: 421 GCNPELKQRSAELLKLCSLNYPDSIFISKCKLTRTIQ 455

BLAST of Lag0004204 vs. NCBI nr
Match: XP_011649559.1 (U-box domain-containing protein 30 isoform X1 [Cucumis sativus] >KAE8652062.1 hypothetical protein Csa_018635 [Cucumis sativus])

HSP 1 Score: 815.5 bits (2105), Expect = 2.3e-232
Identity = 426/457 (93.22%), Postives = 437/457 (95.62%), Query Frame = 0

Query: 1   MPMYQP-SAWRDGDPKLVVGCGAQILDLETVVKDGILGGGG----GGGLISGGGAEKLDL 60
           MPMYQP + WR+GDPKLVVGCG QILDLETVVKDGILGGGG    GGG   GGG EKLDL
Sbjct: 24  MPMYQPATTWREGDPKLVVGCGTQILDLETVVKDGILGGGGGIICGGG--GGGGGEKLDL 83

Query: 61  KEMIEELESSIEVPSVFICPISLEPMQDPVTLCSGQTYERSNILKWFSLGHLTCPTTMQE 120
           KEMIEELESS+EVPSVFICPISLEPMQDPVTLCSGQTYERSNILKWFSLGHLTCPTTMQE
Sbjct: 84  KEMIEELESSVEVPSVFICPISLEPMQDPVTLCSGQTYERSNILKWFSLGHLTCPTTMQE 143

Query: 121 LWDDSVTPNRTLHQLIHTWFSQKYLALKKRSEDVQGRVLELLDSLKKVKGQARVQSLKDL 180
           LWDDSVTPNRTLHQLIHTWFSQKYLALKKRSEDVQGRVLELLDSLKKVKGQARVQSLKDL
Sbjct: 144 LWDDSVTPNRTLHQLIHTWFSQKYLALKKRSEDVQGRVLELLDSLKKVKGQARVQSLKDL 203

Query: 181 RHVVATHSSAKKMVVDNGGVALISSLLGSFTSHAVGSEAVGILVNLDLDSELKKNLIQPT 240
           RH+V THSSAKKMVVDNGGVALISSLLGSFTSHAVGSEAVGILVNLDL+SELKKNLIQPT
Sbjct: 204 RHIVITHSSAKKMVVDNGGVALISSLLGSFTSHAVGSEAVGILVNLDLNSELKKNLIQPT 263

Query: 241 RMSLIADILNEGSIETKINCTKFIKMLIEDDDLRTENVSSLSLLVGLLRMVKDQRHPNGV 300
           R+SLIADILNEGSIETKINCTKF KMLI  +DL+TE+VSSLSLLVGLLRMVKDQRHPNGV
Sbjct: 264 RISLIADILNEGSIETKINCTKFFKMLIVGEDLKTEDVSSLSLLVGLLRMVKDQRHPNGV 323

Query: 301 TAGLSLLRTLCSNESIRKQLVSIGAVAKLGGALSGLNADCLESALYILDVLSSLPEGRLA 360
           TAGLSLLRTLCSNE IRKQLVSIGAVAKLGG LS LN DCLESALYILDVLSSLPEGRLA
Sbjct: 324 TAGLSLLRTLCSNEPIRKQLVSIGAVAKLGGTLSNLNTDCLESALYILDVLSSLPEGRLA 383

Query: 361 LKDCEDTIRNLVKLLMKVSEICTQFALSILWAVFKLAPEECAVLAVEAGLAAKLLLVIQS 420
           LK+CEDTIRNLVKLLMKVSEICTQFALSILWAVFKLAPEECAVLAVEAGLAAKLLLVIQS
Sbjct: 384 LKNCEDTIRNLVKLLMKVSEICTQFALSILWAVFKLAPEECAVLAVEAGLAAKLLLVIQS 443

Query: 421 GCNKELKQRSSELLKLCSLNYPDSIFISKCKLTRTIQ 453
           GCN ELKQRS+ELLKLCSLNYPDSIFISKCKLTRTIQ
Sbjct: 444 GCNPELKQRSAELLKLCSLNYPDSIFISKCKLTRTIQ 478

BLAST of Lag0004204 vs. NCBI nr
Match: XP_008444531.1 (PREDICTED: U-box domain-containing protein 30-like [Cucumis melo] >KAA0060963.1 U-box domain-containing protein 30-like [Cucumis melo var. makuwa])

HSP 1 Score: 814.7 bits (2103), Expect = 4.0e-232
Identity = 428/457 (93.65%), Postives = 438/457 (95.84%), Query Frame = 0

Query: 1   MPMYQP-SAWRDGDPKLVVGCGAQILDLETVVKDGILGGGG----GGGLISGGGAEKLDL 60
           MPMYQP + WR+GDPKLVVGCG QILDLETVVKDGILGGGG    GGG   GGG EKLDL
Sbjct: 1   MPMYQPATTWREGDPKLVVGCGTQILDLETVVKDGILGGGGGIICGGG--GGGGGEKLDL 60

Query: 61  KEMIEELESSIEVPSVFICPISLEPMQDPVTLCSGQTYERSNILKWFSLGHLTCPTTMQE 120
           KEMIEELESSIEVPSVFICPISLEPMQDPVTLCSGQTYERSNILKWFSLGHLTCPTTMQE
Sbjct: 61  KEMIEELESSIEVPSVFICPISLEPMQDPVTLCSGQTYERSNILKWFSLGHLTCPTTMQE 120

Query: 121 LWDDSVTPNRTLHQLIHTWFSQKYLALKKRSEDVQGRVLELLDSLKKVKGQARVQSLKDL 180
           LWDDSVTPNRTLHQLIHTWFSQKYLALKKRSEDVQGRVLELLDSLKKVKGQARVQSLKDL
Sbjct: 121 LWDDSVTPNRTLHQLIHTWFSQKYLALKKRSEDVQGRVLELLDSLKKVKGQARVQSLKDL 180

Query: 181 RHVVATHSSAKKMVVDNGGVALISSLLGSFTSHAVGSEAVGILVNLDLDSELKKNLIQPT 240
           R +V THSSAKKMVVDNGGVALISSLLGSFTSHAVGSEAVGILVNLDL+SELKKNLIQPT
Sbjct: 181 RLIVITHSSAKKMVVDNGGVALISSLLGSFTSHAVGSEAVGILVNLDLNSELKKNLIQPT 240

Query: 241 RMSLIADILNEGSIETKINCTKFIKMLIEDDDLRTENVSSLSLLVGLLRMVKDQRHPNGV 300
           R+SLIADILNEGSIETKINCTKFIKMLI  +DL+TE+VSSLSLLVGLLRMVKDQRHPNGV
Sbjct: 241 RISLIADILNEGSIETKINCTKFIKMLIVGEDLKTEDVSSLSLLVGLLRMVKDQRHPNGV 300

Query: 301 TAGLSLLRTLCSNESIRKQLVSIGAVAKLGGALSGLNADCLESALYILDVLSSLPEGRLA 360
           TAGLSLLRTLCSNE IRKQLVSIGAVAKLGG LS LNADCLESALYILDVLSSLPEGRLA
Sbjct: 301 TAGLSLLRTLCSNEPIRKQLVSIGAVAKLGGTLSNLNADCLESALYILDVLSSLPEGRLA 360

Query: 361 LKDCEDTIRNLVKLLMKVSEICTQFALSILWAVFKLAPEECAVLAVEAGLAAKLLLVIQS 420
           LK+CEDTIRNLVKLLMKVSEICTQFALSILWAVFKLAPEECAVLAVEAGLAAKLLLVIQS
Sbjct: 361 LKNCEDTIRNLVKLLMKVSEICTQFALSILWAVFKLAPEECAVLAVEAGLAAKLLLVIQS 420

Query: 421 GCNKELKQRSSELLKLCSLNYPDSIFISKCKLTRTIQ 453
           GCN ELKQRS+ELLKLCSLNYPDSIFISKCKLTRTIQ
Sbjct: 421 GCNPELKQRSAELLKLCSLNYPDSIFISKCKLTRTIQ 455

BLAST of Lag0004204 vs. NCBI nr
Match: XP_022962495.1 (U-box domain-containing protein 31-like isoform X1 [Cucurbita moschata])

HSP 1 Score: 793.5 bits (2048), Expect = 9.6e-226
Identity = 413/452 (91.37%), Postives = 428/452 (94.69%), Query Frame = 0

Query: 1   MPMYQPSAWRDGDPKLVVGCGAQILDLETVVKDGILGGGGGGGLISGGGAEKLDLKEMIE 60
           MP+Y PS     DPKL+VGCGA ILDL+TVVKDGILGGGGGG + + GGA+KLDLKEMIE
Sbjct: 1   MPIYHPSICTQRDPKLLVGCGAHILDLQTVVKDGILGGGGGGVIAADGGAQKLDLKEMIE 60

Query: 61  ELESSIEVPSVFICPISLEPMQDPVTLCSGQTYERSNILKWFSLGHLTCPTTMQELWDDS 120
           ELESSIEVPSVFICPISLEPMQDPVTLCSGQTYERSNILKWFSLGHLTCPTTMQELWDDS
Sbjct: 61  ELESSIEVPSVFICPISLEPMQDPVTLCSGQTYERSNILKWFSLGHLTCPTTMQELWDDS 120

Query: 121 VTPNRTLHQLIHTWFSQKYLALKKRSEDVQGRVLELLDSLKKVKGQARVQSLKDLRHVVA 180
           VTPNRTLHQLIHTWFSQKYLALKKRSEDVQGRVLELLDSLKKVKGQARVQSLKDLRHVV 
Sbjct: 121 VTPNRTLHQLIHTWFSQKYLALKKRSEDVQGRVLELLDSLKKVKGQARVQSLKDLRHVVT 180

Query: 181 THSSAKKMVVDNGGVALISSLLGSFTSHAVGSEAVGILVNLDLDSELKKNLIQPTRMSLI 240
            HSSAKKMVVDNGGVALISSLLGSFTSHAVGSE VGILVNLDL+SELKKNLIQPTR+SLI
Sbjct: 181 AHSSAKKMVVDNGGVALISSLLGSFTSHAVGSEGVGILVNLDLNSELKKNLIQPTRISLI 240

Query: 241 ADILNEGSIETKINCTKFIKMLIEDDDLRTENVSSLSLLVGLLRMVKDQRHPNGVTAGLS 300
           ADILNEGSIETKINCTKFIKML E D    ENVSSLSLLVGLLRMVKDQRHPNGVTA L+
Sbjct: 241 ADILNEGSIETKINCTKFIKMLTEGDASTIENVSSLSLLVGLLRMVKDQRHPNGVTAALA 300

Query: 301 LLRTLCSNESIRKQLVSIGAVAKLGGALSGLNADCLESALYILDVLSSLPEGRLALKDCE 360
           LLRTLCSNESIRKQLV IGAVAKLGGALSGLNA+ LESALYILDVLSSLPEGRLALK+CE
Sbjct: 301 LLRTLCSNESIRKQLVGIGAVAKLGGALSGLNAESLESALYILDVLSSLPEGRLALKNCE 360

Query: 361 DTIRNLVKLLMKVSEICTQFALSILWAVFKLAPEECAVLAVEAGLAAKLLLVIQSGCNKE 420
           DT+RNLVKLLMKVSEICT+FALSILWAVFKLAPEEC+VLAVEAGLAAKLLLVIQSG N E
Sbjct: 361 DTVRNLVKLLMKVSEICTRFALSILWAVFKLAPEECSVLAVEAGLAAKLLLVIQSGFNPE 420

Query: 421 LKQRSSELLKLCSLNYPDSIFISKCKLTRTIQ 453
           LKQRS+ELLKLCSLNYPDSIFISKCKLTRTIQ
Sbjct: 421 LKQRSAELLKLCSLNYPDSIFISKCKLTRTIQ 452

BLAST of Lag0004204 vs. ExPASy Swiss-Prot
Match: Q058P4 (U-box domain-containing protein 30 OS=Arabidopsis thaliana OX=3702 GN=PUB30 PE=1 SV=1)

HSP 1 Score: 555.4 bits (1430), Expect = 5.7e-157
Identity = 291/452 (64.38%), Postives = 360/452 (79.65%), Query Frame = 0

Query: 1   MPMYQPSAWRDGDPKLVVGCGAQILDLETVVKDGILGGGGGGGLISGGGAEKLDLKEMIE 60
           MPM+QP   RDG      G   Q+LDL+T VKDG+LGG  GGG+  G   EKLDLK+MI+
Sbjct: 1   MPMFQPLK-RDGLIGFEGGGDGQVLDLDTAVKDGVLGGVNGGGV--GVVDEKLDLKKMIK 60

Query: 61  ELESSIEVPSVFICPISLEPMQDPVTLCSGQTYERSNILKWFSLGHLTCPTTMQELWDDS 120
           EL+   ++PSVFICPISLEPMQDPVTLC+GQTYER NI KWF+LGHLTCPTTMQELWDD+
Sbjct: 61  ELDLQ-DIPSVFICPISLEPMQDPVTLCTGQTYERLNIHKWFNLGHLTCPTTMQELWDDT 120

Query: 121 VTPNRTLHQLIHTWFSQKYLALKKRSEDVQGRVLELLDSLKKVKGQARVQSLKDLRHVVA 180
           VTPN+TLH LI+TWFSQKY+ +KKRSEDVQGR +E+L +LKK KGQARV +L +L+ +V 
Sbjct: 121 VTPNKTLHHLIYTWFSQKYVLMKKRSEDVQGRAIEILGTLKKAKGQARVHALSELKQIVI 180

Query: 181 THSSAKKMVVDNGGVALISSLLGSFTSHAVGSEAVGILVNLDLDSELKKNLIQPTRMSLI 240
            H  A+K VV+ GGV++ISSLLG FTSHAVGSE V ILV+LDLDS+ K  L+QP ++SLI
Sbjct: 181 AHLMARKTVVEEGGVSVISSLLGPFTSHAVGSEVVAILVSLDLDSDSKSGLMQPAKVSLI 240

Query: 241 ADILNEGSIETKINCTKFIKMLIEDDDLRTENVSSLSLLVGLLRMVKDQRHPNGVTAGLS 300
            D+LN+GS ETKINC + I+ L+E+   R E VSS SLLVGL+R+VKD+RH NGV+  L 
Sbjct: 241 VDMLNDGSNETKINCARLIRGLVEEKGFRAELVSSHSLLVGLMRLVKDKRHRNGVSPALR 300

Query: 301 LLRTLCSNESIRKQLVSIGAVAKLGGALSGLNADCLESALYILDVLSSLPEGRLALKDCE 360
           LL+ +  ++ +R  +VSIGAV +L   L  L+ +CLE AL++LD L +  EGR+A+KD  
Sbjct: 301 LLKPISVHKQVRSLMVSIGAVPQLVDILPSLDPECLELALFVLDALCTDVEGRVAVKDSA 360

Query: 361 DTIRNLVKLLMKVSEICTQFALSILWAVFKLAPEECAVLAVEAGLAAKLLLVIQSGCNKE 420
           +TI   V++LM+VSE CT +ALSILW+V KLAPEEC+ LAVE GLAAKLLLVIQSGC+  
Sbjct: 361 NTIPYTVRVLMRVSENCTNYALSILWSVCKLAPEECSPLAVEVGLAAKLLLVIQSGCDAA 420

Query: 421 LKQRSSELLKLCSLNYPDSIFISKCKLTRTIQ 453
           LKQRS+ELLKLCSL+Y D++FISKCKLTRTIQ
Sbjct: 421 LKQRSAELLKLCSLHYSDTMFISKCKLTRTIQ 448

BLAST of Lag0004204 vs. ExPASy Swiss-Prot
Match: Q9FHN9 (U-box domain-containing protein 31 OS=Arabidopsis thaliana OX=3702 GN=PUB31 PE=2 SV=1)

HSP 1 Score: 550.8 bits (1418), Expect = 1.4e-155
Identity = 293/453 (64.68%), Postives = 358/453 (79.03%), Query Frame = 0

Query: 1   MPMYQPSAWRDGDPKLVVGCGAQILDLETVVKDGILGGGGGGGL-ISGGGAEKLDLKEMI 60
           MPM+QPS  ++G      G    ILDL + VKDG+LGGG G  L +     +KLDLKEMI
Sbjct: 1   MPMFQPS--KNG------GFDGHILDLHSAVKDGVLGGGDGKFLVVVTDEKKKLDLKEMI 60

Query: 61  EELESSIEVPSVFICPISLEPMQDPVTLCSGQTYERSNILKWFSLGHLTCPTTMQELWDD 120
            ELE   E+PSVFICPISLEPMQDPVTLC+GQTYERSNILKWF++GH TCPTTMQELWDD
Sbjct: 61  SELELP-EIPSVFICPISLEPMQDPVTLCTGQTYERSNILKWFNIGHCTCPTTMQELWDD 120

Query: 121 SVTPNRTLHQLIHTWFSQKYLALKKRSEDVQGRVLELLDSLKKVKGQARVQSLKDLRHVV 180
            VTPN+TLHQLI+TWFSQKY+ +KKRSEDVQGR +E+L +L+K KG+A+V +L +L+ VV
Sbjct: 121 LVTPNKTLHQLIYTWFSQKYVLMKKRSEDVQGRAIEILGTLRKAKGKAKVHALSELKQVV 180

Query: 181 ATHSSAKKMVVDNGGVALISSLLGSFTSHAVGSEAVGILVNLDLDSELKKNLIQPTRMSL 240
             H+ AKK VVD GGV +ISSLL  FTSHAVGSEA+ ILVNL+LDS+ K  L+QP R+SL
Sbjct: 181 MAHAIAKKTVVDEGGVFVISSLLSPFTSHAVGSEAIAILVNLELDSDSKAGLMQPARVSL 240

Query: 241 IADILNEGSIETKINCTKFIKMLIEDDDLRTENVSSLSLLVGLLRMVKDQRHPNGVTAGL 300
           + D+LN+GSIETKINC + I  L+E+   R E VSS SLLVGL+R+VKD+R  NGV+  L
Sbjct: 241 MVDMLNDGSIETKINCARLIGRLVEEKGFRAELVSSHSLLVGLMRLVKDRRRRNGVSPAL 300

Query: 301 SLLRTLCSNESIRKQLVSIGAVAKLGGALSGLNADCLESALYILDVLSSLPEGRLALKDC 360
           +LL+++  ++ +R  LV IGAV +L   L  L+ +CLESAL++LD L    EGR+ALKD 
Sbjct: 301 TLLKSVSVHKQVRNLLVRIGAVPQLVDVLPCLDVECLESALFVLDSLCLESEGRIALKDS 360

Query: 361 EDTIRNLVKLLMKVSEICTQFALSILWAVFKLAPEECAVLAVEAGLAAKLLLVIQSGCNK 420
            +TI + V+LLMKVSE CT +A+SILW+V KLA EEC+ LAVE GLAAKLLLVIQSGC+ 
Sbjct: 361 VNTIPHTVRLLMKVSEKCTNYAISILWSVCKLASEECSSLAVEVGLAAKLLLVIQSGCDP 420

Query: 421 ELKQRSSELLKLCSLNYPDSIFISKCKLTRTIQ 453
            LKQRS+ELLKLCSL+Y DS+FISKCKLTRTIQ
Sbjct: 421 ALKQRSAELLKLCSLHYSDSMFISKCKLTRTIQ 444

BLAST of Lag0004204 vs. ExPASy Swiss-Prot
Match: Q10PI9 (U-box domain-containing protein 75 OS=Oryza sativa subsp. japonica OX=39947 GN=TUD1 PE=1 SV=1)

HSP 1 Score: 484.6 bits (1246), Expect = 1.2e-135
Identity = 251/451 (55.65%), Postives = 339/451 (75.17%), Query Frame = 0

Query: 20  CGAQILDLETVVKDGILGGGG--GGGLISGGGAEKLDLKEMIEELESSIE------VPSV 79
           CG Q+LD++T +KDGILGGG   G      GG + ++L++M++EL+++ +      VP+V
Sbjct: 9   CGGQVLDIDTALKDGILGGGPELGDAAAGDGGKQPVELRKMMDELDAAGDGGGDEAVPAV 68

Query: 80  FICPISLEPMQDPVTLCSGQTYERSNILKWFSLGHLTCPTTMQELWDDSVTPNRTLHQLI 139
           FICPISLEPM DPVTLC+GQTYE +NI +W +LGH TCPTTMQELWD +  PN TL QLI
Sbjct: 69  FICPISLEPMVDPVTLCTGQTYESANISRWLALGHRTCPTTMQELWDVTPIPNTTLRQLI 128

Query: 140 HTWFSQKYLALKKRSEDVQGRVLELLDSLK--------KVKGQARVQSLKDLRHVVATHS 199
             WFS++Y   KKRS D  GR  EL+ +L+         +KGQARV +L++LR + A H 
Sbjct: 129 AAWFSRRYTRFKKRSADFHGRAAELVHALRGTAVPKRQPLKGQARVAALRELRSLAAAHQ 188

Query: 200 SAKKMVVDNGGVALISSLLGSFTSHAVGSEAVGILVN-LDLDSELKKNLIQPTRMSLIAD 259
           S  K + + GGV L++SLLG FTSHAVGSEAV ILV+ + LD++ K  L+QP ++SL+ D
Sbjct: 189 SVTKAIAEAGGVGLLTSLLGPFTSHAVGSEAVAILVSGVPLDADAKAALMQPAKVSLLVD 248

Query: 260 ILNEGSIETKINCTKFIKMLIEDDDLRTENVSSLSLLVGLLRMVKDQRHPNGVTAGLSLL 319
           +LNEG+++TKINC + I++L+E+   R + V+SLSLLVG++R+V+D+RHP+GV AGL LL
Sbjct: 249 MLNEGAVDTKINCVRLIRILMEEKGFRPDTVASLSLLVGVMRLVRDKRHPDGVAAGLELL 308

Query: 320 RTLCS-NESIRKQLVSIGAVAKLGGALSGLNADCLESALYILDVLSSLPEGRLALKDCED 379
            ++C+ ++  R  +VSIGAV +L   L  L  +C+E AL ILD L+++PEGR+ALKDC  
Sbjct: 309 NSICAVHKPARSLIVSIGAVPQLVELLPELPTECVEPALDILDALAAVPEGRIALKDCPR 368

Query: 380 TIRNLVKLLMKVSEICTQFALSILWAVFKLAPEECAVLAVEAGLAAKLLLVIQSGCNKEL 439
           TI N V+LLM+VSE CT+ ALS+LW V ++APEECA  A++AGL AKLLLVIQSGC  EL
Sbjct: 369 TITNAVRLLMRVSEACTRRALSMLWVVCRMAPEECAPAALDAGLGAKLLLVIQSGCGPEL 428

Query: 440 KQRSSELLKLCSLNYPDSIFISKCKLTRTIQ 453
           KQ++SELLKLC++N   ++FISKCKLT+TIQ
Sbjct: 429 KQQASELLKLCTMNCTSTVFISKCKLTKTIQ 459

BLAST of Lag0004204 vs. ExPASy Swiss-Prot
Match: Q9LT79 (U-box domain-containing protein 25 OS=Arabidopsis thaliana OX=3702 GN=PUB25 PE=2 SV=1)

HSP 1 Score: 153.7 bits (387), Expect = 5.0e-36
Identity = 126/405 (31.11%), Postives = 203/405 (50.12%), Query Frame = 0

Query: 59  IEELESSIEVPSVFICPISLEPMQDPVTLCSGQTYERSNILKWFSLG-HLTCPTTMQELW 118
           IE L+  I++P  F CPISLE MQDPVT+C+GQTY+R++I  W S+G + TCP T   L 
Sbjct: 5   IEPLDLGIQIPYHFRCPISLELMQDPVTVCTGQTYDRASIESWVSIGNNTTCPVTRAPLS 64

Query: 119 DDSVTPNRTLHQLIHTWFSQKYLALKKRSEDVQ-----------GRVLELLDSLKKVKG- 178
           D ++ PN TL +LI  W       +  RS  V+             V  LL     + G 
Sbjct: 65  DFTLIPNHTLRRLIQEW------CVANRSNGVERIPTPKQPADPTSVRALLSQASAITGT 124

Query: 179 ----QARVQSLKDLRHVVATHSSAKKMV-VDNGGVALISSLLGSFTSHAVGSEAVGILVN 238
               ++R  +L+ LR         + ++   N    LI  L    TS  + SE++ +LV 
Sbjct: 125 HVSVRSRAAALRRLRGFARDSDKNRVLIAAHNATEILIKILFSETTSSELVSESLALLVM 184

Query: 239 LDL--DSELKKNLIQPTRMSLIADILNEGSIETKINCTKFIKML---IEDDDLRTENVSS 298
           L +   ++       P R+  +  +L + SIET++N    I+++    +  DL+    +S
Sbjct: 185 LPITEPNQFVSISSDPGRVEFLTRLLFDSSIETRVNAAALIEIVSTGTKSADLKGSISNS 244

Query: 299 LSLLVGLLRMVKDQ-RHPNGVTAGLSLLRTLCSNESIRKQLVSIGAVAKLGGALSGLNAD 358
            S+  G+L ++++       +  G+  L  LCS +S R   ++ GA   L   L+     
Sbjct: 245 ESVFEGVLDLLRNPISSRRALKIGIKTLFALCSVKSTRHIAITAGAPEILIDRLAADFDR 304

Query: 359 C-LESALYILDVLSSLPEGRLALKDCEDTIRNLVKLLMKVSEICTQFALSILWAVFKLAP 418
           C  E AL  +++L   PEG  A  +   T+  LVK +++VS+  T++A   L A+   A 
Sbjct: 305 CDTERALATVELLCRTPEGCAAFGEHALTVPLLVKTILRVSDRATEYAAGALLALC-TAE 364

Query: 419 EECAVLAVEAGLAAKLLLVIQSGCNKELKQRSSELLKLCSLNYPD 439
           E     A  AG+  +LLL++QS C +  K+++ +LLKL   ++PD
Sbjct: 365 ERWREEAAGAGVVVQLLLMVQSECTERAKKKAQKLLKLLRDSWPD 402

BLAST of Lag0004204 vs. ExPASy Swiss-Prot
Match: Q9FXA4 (U-box domain-containing protein 26 OS=Arabidopsis thaliana OX=3702 GN=PUB26 PE=2 SV=1)

HSP 1 Score: 149.1 bits (375), Expect = 1.2e-34
Identity = 119/408 (29.17%), Postives = 204/408 (50.00%), Query Frame = 0

Query: 59  IEELESSIEVPSVFICPISLEPMQDPVTLCSGQTYERSNILKWFSLGHLTCPTTMQELWD 118
           +E L+  I++P  F CPISL+ M DPVT+ +GQTY+R++I  W ++G+ TCP T   L D
Sbjct: 5   LEPLDLGIQIPYHFRCPISLDLMSDPVTISTGQTYDRTSIDSWIAMGNTTCPVTRVALSD 64

Query: 119 DSVTPNRTLHQLIHTWFSQKYLALKKRSEDVQG-----------RVLELLDSLKKVKG-- 178
            ++ PN TL +LI  W       +  RS  V+             V  LL     + G  
Sbjct: 65  FTLIPNHTLRRLIQEW------CVANRSNGVERIPTPKQPADPISVRSLLSQASAITGTH 124

Query: 179 ---QARVQSLKDLRHVVATHSSAKKMVVDNGGVALISSLL------GSFTSHAVGSEAVG 238
              ++R  +++ LR +       + ++  +    ++  +L       S +S  V SE++ 
Sbjct: 125 VSVRSRAAAIRRLRGLARDSEKNRVLIAGHNAREILVRILFADIETTSLSSELV-SESLA 184

Query: 239 ILVNLDL-DSELKKNLIQPTRMSLIADILNEGSIETKINCTKFIKMLI---EDDDLRTEN 298
           +LV L + ++E +     P+R+  +  +L + SIE ++N    I+M++   +  DL+   
Sbjct: 185 LLVLLHMTETECEAVASDPSRVGFMTRLLFDSSIEIRVNAAALIEMVLTGAKSMDLKLII 244

Query: 299 VSSLSLLVGLLRMVKDQ-RHPNGVTAGLSLLRTLCSNESIRKQLVSIGAVAKLGGALSGL 358
             S S+  G+L ++K+       +  G+  +  LC  +  R   +S GA   L   L+  
Sbjct: 245 SGSDSIFEGVLDLLKNPISSRRALKIGIKAIFALCLVKQTRHLAISAGAPGILIDRLAAD 304

Query: 359 NADC-LESALYILDVLSSLPEGRLALKDCEDTIRNLVKLLMKVSEICTQFALSILWAVFK 418
              C  E  L  +++L  LPEG  A  +   T+  +VK +++VS+  T++A   L A+  
Sbjct: 305 FDRCDTERGLATVELLCRLPEGCAAFGEHALTVPLMVKTILRVSDRATEYAAGALLALC- 364

Query: 419 LAPEECAVLAVEAGLAAKLLLVIQSGCNKELKQRSSELLKLCSLNYPD 439
            A E C   A  AGL  +LLL++QS C +  K+++  LLKL   ++PD
Sbjct: 365 TAEERCRDEAAAAGLVTQLLLLVQSDCTERAKRKAQMLLKLLRDSWPD 404

BLAST of Lag0004204 vs. ExPASy TrEMBL
Match: A0A0A0LKY4 (RING-type E3 ubiquitin transferase OS=Cucumis sativus OX=3659 GN=Csa_2G354100 PE=4 SV=1)

HSP 1 Score: 815.5 bits (2105), Expect = 1.1e-232
Identity = 426/457 (93.22%), Postives = 437/457 (95.62%), Query Frame = 0

Query: 1   MPMYQP-SAWRDGDPKLVVGCGAQILDLETVVKDGILGGGG----GGGLISGGGAEKLDL 60
           MPMYQP + WR+GDPKLVVGCG QILDLETVVKDGILGGGG    GGG   GGG EKLDL
Sbjct: 1   MPMYQPATTWREGDPKLVVGCGTQILDLETVVKDGILGGGGGIICGGG--GGGGGEKLDL 60

Query: 61  KEMIEELESSIEVPSVFICPISLEPMQDPVTLCSGQTYERSNILKWFSLGHLTCPTTMQE 120
           KEMIEELESS+EVPSVFICPISLEPMQDPVTLCSGQTYERSNILKWFSLGHLTCPTTMQE
Sbjct: 61  KEMIEELESSVEVPSVFICPISLEPMQDPVTLCSGQTYERSNILKWFSLGHLTCPTTMQE 120

Query: 121 LWDDSVTPNRTLHQLIHTWFSQKYLALKKRSEDVQGRVLELLDSLKKVKGQARVQSLKDL 180
           LWDDSVTPNRTLHQLIHTWFSQKYLALKKRSEDVQGRVLELLDSLKKVKGQARVQSLKDL
Sbjct: 121 LWDDSVTPNRTLHQLIHTWFSQKYLALKKRSEDVQGRVLELLDSLKKVKGQARVQSLKDL 180

Query: 181 RHVVATHSSAKKMVVDNGGVALISSLLGSFTSHAVGSEAVGILVNLDLDSELKKNLIQPT 240
           RH+V THSSAKKMVVDNGGVALISSLLGSFTSHAVGSEAVGILVNLDL+SELKKNLIQPT
Sbjct: 181 RHIVITHSSAKKMVVDNGGVALISSLLGSFTSHAVGSEAVGILVNLDLNSELKKNLIQPT 240

Query: 241 RMSLIADILNEGSIETKINCTKFIKMLIEDDDLRTENVSSLSLLVGLLRMVKDQRHPNGV 300
           R+SLIADILNEGSIETKINCTKF KMLI  +DL+TE+VSSLSLLVGLLRMVKDQRHPNGV
Sbjct: 241 RISLIADILNEGSIETKINCTKFFKMLIVGEDLKTEDVSSLSLLVGLLRMVKDQRHPNGV 300

Query: 301 TAGLSLLRTLCSNESIRKQLVSIGAVAKLGGALSGLNADCLESALYILDVLSSLPEGRLA 360
           TAGLSLLRTLCSNE IRKQLVSIGAVAKLGG LS LN DCLESALYILDVLSSLPEGRLA
Sbjct: 301 TAGLSLLRTLCSNEPIRKQLVSIGAVAKLGGTLSNLNTDCLESALYILDVLSSLPEGRLA 360

Query: 361 LKDCEDTIRNLVKLLMKVSEICTQFALSILWAVFKLAPEECAVLAVEAGLAAKLLLVIQS 420
           LK+CEDTIRNLVKLLMKVSEICTQFALSILWAVFKLAPEECAVLAVEAGLAAKLLLVIQS
Sbjct: 361 LKNCEDTIRNLVKLLMKVSEICTQFALSILWAVFKLAPEECAVLAVEAGLAAKLLLVIQS 420

Query: 421 GCNKELKQRSSELLKLCSLNYPDSIFISKCKLTRTIQ 453
           GCN ELKQRS+ELLKLCSLNYPDSIFISKCKLTRTIQ
Sbjct: 421 GCNPELKQRSAELLKLCSLNYPDSIFISKCKLTRTIQ 455

BLAST of Lag0004204 vs. ExPASy TrEMBL
Match: A0A5A7UYA8 (RING-type E3 ubiquitin transferase OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold501G00980 PE=4 SV=1)

HSP 1 Score: 814.7 bits (2103), Expect = 1.9e-232
Identity = 428/457 (93.65%), Postives = 438/457 (95.84%), Query Frame = 0

Query: 1   MPMYQP-SAWRDGDPKLVVGCGAQILDLETVVKDGILGGGG----GGGLISGGGAEKLDL 60
           MPMYQP + WR+GDPKLVVGCG QILDLETVVKDGILGGGG    GGG   GGG EKLDL
Sbjct: 1   MPMYQPATTWREGDPKLVVGCGTQILDLETVVKDGILGGGGGIICGGG--GGGGGEKLDL 60

Query: 61  KEMIEELESSIEVPSVFICPISLEPMQDPVTLCSGQTYERSNILKWFSLGHLTCPTTMQE 120
           KEMIEELESSIEVPSVFICPISLEPMQDPVTLCSGQTYERSNILKWFSLGHLTCPTTMQE
Sbjct: 61  KEMIEELESSIEVPSVFICPISLEPMQDPVTLCSGQTYERSNILKWFSLGHLTCPTTMQE 120

Query: 121 LWDDSVTPNRTLHQLIHTWFSQKYLALKKRSEDVQGRVLELLDSLKKVKGQARVQSLKDL 180
           LWDDSVTPNRTLHQLIHTWFSQKYLALKKRSEDVQGRVLELLDSLKKVKGQARVQSLKDL
Sbjct: 121 LWDDSVTPNRTLHQLIHTWFSQKYLALKKRSEDVQGRVLELLDSLKKVKGQARVQSLKDL 180

Query: 181 RHVVATHSSAKKMVVDNGGVALISSLLGSFTSHAVGSEAVGILVNLDLDSELKKNLIQPT 240
           R +V THSSAKKMVVDNGGVALISSLLGSFTSHAVGSEAVGILVNLDL+SELKKNLIQPT
Sbjct: 181 RLIVITHSSAKKMVVDNGGVALISSLLGSFTSHAVGSEAVGILVNLDLNSELKKNLIQPT 240

Query: 241 RMSLIADILNEGSIETKINCTKFIKMLIEDDDLRTENVSSLSLLVGLLRMVKDQRHPNGV 300
           R+SLIADILNEGSIETKINCTKFIKMLI  +DL+TE+VSSLSLLVGLLRMVKDQRHPNGV
Sbjct: 241 RISLIADILNEGSIETKINCTKFIKMLIVGEDLKTEDVSSLSLLVGLLRMVKDQRHPNGV 300

Query: 301 TAGLSLLRTLCSNESIRKQLVSIGAVAKLGGALSGLNADCLESALYILDVLSSLPEGRLA 360
           TAGLSLLRTLCSNE IRKQLVSIGAVAKLGG LS LNADCLESALYILDVLSSLPEGRLA
Sbjct: 301 TAGLSLLRTLCSNEPIRKQLVSIGAVAKLGGTLSNLNADCLESALYILDVLSSLPEGRLA 360

Query: 361 LKDCEDTIRNLVKLLMKVSEICTQFALSILWAVFKLAPEECAVLAVEAGLAAKLLLVIQS 420
           LK+CEDTIRNLVKLLMKVSEICTQFALSILWAVFKLAPEECAVLAVEAGLAAKLLLVIQS
Sbjct: 361 LKNCEDTIRNLVKLLMKVSEICTQFALSILWAVFKLAPEECAVLAVEAGLAAKLLLVIQS 420

Query: 421 GCNKELKQRSSELLKLCSLNYPDSIFISKCKLTRTIQ 453
           GCN ELKQRS+ELLKLCSLNYPDSIFISKCKLTRTIQ
Sbjct: 421 GCNPELKQRSAELLKLCSLNYPDSIFISKCKLTRTIQ 455

BLAST of Lag0004204 vs. ExPASy TrEMBL
Match: A0A1S3BA20 (RING-type E3 ubiquitin transferase OS=Cucumis melo OX=3656 GN=LOC103487822 PE=4 SV=1)

HSP 1 Score: 814.7 bits (2103), Expect = 1.9e-232
Identity = 428/457 (93.65%), Postives = 438/457 (95.84%), Query Frame = 0

Query: 1   MPMYQP-SAWRDGDPKLVVGCGAQILDLETVVKDGILGGGG----GGGLISGGGAEKLDL 60
           MPMYQP + WR+GDPKLVVGCG QILDLETVVKDGILGGGG    GGG   GGG EKLDL
Sbjct: 1   MPMYQPATTWREGDPKLVVGCGTQILDLETVVKDGILGGGGGIICGGG--GGGGGEKLDL 60

Query: 61  KEMIEELESSIEVPSVFICPISLEPMQDPVTLCSGQTYERSNILKWFSLGHLTCPTTMQE 120
           KEMIEELESSIEVPSVFICPISLEPMQDPVTLCSGQTYERSNILKWFSLGHLTCPTTMQE
Sbjct: 61  KEMIEELESSIEVPSVFICPISLEPMQDPVTLCSGQTYERSNILKWFSLGHLTCPTTMQE 120

Query: 121 LWDDSVTPNRTLHQLIHTWFSQKYLALKKRSEDVQGRVLELLDSLKKVKGQARVQSLKDL 180
           LWDDSVTPNRTLHQLIHTWFSQKYLALKKRSEDVQGRVLELLDSLKKVKGQARVQSLKDL
Sbjct: 121 LWDDSVTPNRTLHQLIHTWFSQKYLALKKRSEDVQGRVLELLDSLKKVKGQARVQSLKDL 180

Query: 181 RHVVATHSSAKKMVVDNGGVALISSLLGSFTSHAVGSEAVGILVNLDLDSELKKNLIQPT 240
           R +V THSSAKKMVVDNGGVALISSLLGSFTSHAVGSEAVGILVNLDL+SELKKNLIQPT
Sbjct: 181 RLIVITHSSAKKMVVDNGGVALISSLLGSFTSHAVGSEAVGILVNLDLNSELKKNLIQPT 240

Query: 241 RMSLIADILNEGSIETKINCTKFIKMLIEDDDLRTENVSSLSLLVGLLRMVKDQRHPNGV 300
           R+SLIADILNEGSIETKINCTKFIKMLI  +DL+TE+VSSLSLLVGLLRMVKDQRHPNGV
Sbjct: 241 RISLIADILNEGSIETKINCTKFIKMLIVGEDLKTEDVSSLSLLVGLLRMVKDQRHPNGV 300

Query: 301 TAGLSLLRTLCSNESIRKQLVSIGAVAKLGGALSGLNADCLESALYILDVLSSLPEGRLA 360
           TAGLSLLRTLCSNE IRKQLVSIGAVAKLGG LS LNADCLESALYILDVLSSLPEGRLA
Sbjct: 301 TAGLSLLRTLCSNEPIRKQLVSIGAVAKLGGTLSNLNADCLESALYILDVLSSLPEGRLA 360

Query: 361 LKDCEDTIRNLVKLLMKVSEICTQFALSILWAVFKLAPEECAVLAVEAGLAAKLLLVIQS 420
           LK+CEDTIRNLVKLLMKVSEICTQFALSILWAVFKLAPEECAVLAVEAGLAAKLLLVIQS
Sbjct: 361 LKNCEDTIRNLVKLLMKVSEICTQFALSILWAVFKLAPEECAVLAVEAGLAAKLLLVIQS 420

Query: 421 GCNKELKQRSSELLKLCSLNYPDSIFISKCKLTRTIQ 453
           GCN ELKQRS+ELLKLCSLNYPDSIFISKCKLTRTIQ
Sbjct: 421 GCNPELKQRSAELLKLCSLNYPDSIFISKCKLTRTIQ 455

BLAST of Lag0004204 vs. ExPASy TrEMBL
Match: A0A6J1HFA1 (RING-type E3 ubiquitin transferase OS=Cucurbita moschata OX=3662 GN=LOC111462914 PE=4 SV=1)

HSP 1 Score: 793.5 bits (2048), Expect = 4.6e-226
Identity = 413/452 (91.37%), Postives = 428/452 (94.69%), Query Frame = 0

Query: 1   MPMYQPSAWRDGDPKLVVGCGAQILDLETVVKDGILGGGGGGGLISGGGAEKLDLKEMIE 60
           MP+Y PS     DPKL+VGCGA ILDL+TVVKDGILGGGGGG + + GGA+KLDLKEMIE
Sbjct: 1   MPIYHPSICTQRDPKLLVGCGAHILDLQTVVKDGILGGGGGGVIAADGGAQKLDLKEMIE 60

Query: 61  ELESSIEVPSVFICPISLEPMQDPVTLCSGQTYERSNILKWFSLGHLTCPTTMQELWDDS 120
           ELESSIEVPSVFICPISLEPMQDPVTLCSGQTYERSNILKWFSLGHLTCPTTMQELWDDS
Sbjct: 61  ELESSIEVPSVFICPISLEPMQDPVTLCSGQTYERSNILKWFSLGHLTCPTTMQELWDDS 120

Query: 121 VTPNRTLHQLIHTWFSQKYLALKKRSEDVQGRVLELLDSLKKVKGQARVQSLKDLRHVVA 180
           VTPNRTLHQLIHTWFSQKYLALKKRSEDVQGRVLELLDSLKKVKGQARVQSLKDLRHVV 
Sbjct: 121 VTPNRTLHQLIHTWFSQKYLALKKRSEDVQGRVLELLDSLKKVKGQARVQSLKDLRHVVT 180

Query: 181 THSSAKKMVVDNGGVALISSLLGSFTSHAVGSEAVGILVNLDLDSELKKNLIQPTRMSLI 240
            HSSAKKMVVDNGGVALISSLLGSFTSHAVGSE VGILVNLDL+SELKKNLIQPTR+SLI
Sbjct: 181 AHSSAKKMVVDNGGVALISSLLGSFTSHAVGSEGVGILVNLDLNSELKKNLIQPTRISLI 240

Query: 241 ADILNEGSIETKINCTKFIKMLIEDDDLRTENVSSLSLLVGLLRMVKDQRHPNGVTAGLS 300
           ADILNEGSIETKINCTKFIKML E D    ENVSSLSLLVGLLRMVKDQRHPNGVTA L+
Sbjct: 241 ADILNEGSIETKINCTKFIKMLTEGDASTIENVSSLSLLVGLLRMVKDQRHPNGVTAALA 300

Query: 301 LLRTLCSNESIRKQLVSIGAVAKLGGALSGLNADCLESALYILDVLSSLPEGRLALKDCE 360
           LLRTLCSNESIRKQLV IGAVAKLGGALSGLNA+ LESALYILDVLSSLPEGRLALK+CE
Sbjct: 301 LLRTLCSNESIRKQLVGIGAVAKLGGALSGLNAESLESALYILDVLSSLPEGRLALKNCE 360

Query: 361 DTIRNLVKLLMKVSEICTQFALSILWAVFKLAPEECAVLAVEAGLAAKLLLVIQSGCNKE 420
           DT+RNLVKLLMKVSEICT+FALSILWAVFKLAPEEC+VLAVEAGLAAKLLLVIQSG N E
Sbjct: 361 DTVRNLVKLLMKVSEICTRFALSILWAVFKLAPEECSVLAVEAGLAAKLLLVIQSGFNPE 420

Query: 421 LKQRSSELLKLCSLNYPDSIFISKCKLTRTIQ 453
           LKQRS+ELLKLCSLNYPDSIFISKCKLTRTIQ
Sbjct: 421 LKQRSAELLKLCSLNYPDSIFISKCKLTRTIQ 452

BLAST of Lag0004204 vs. ExPASy TrEMBL
Match: A0A6J1K7W0 (RING-type E3 ubiquitin transferase OS=Cucurbita maxima OX=3661 GN=LOC111492478 PE=4 SV=1)

HSP 1 Score: 789.3 bits (2037), Expect = 8.7e-225
Identity = 411/452 (90.93%), Postives = 426/452 (94.25%), Query Frame = 0

Query: 1   MPMYQPSAWRDGDPKLVVGCGAQILDLETVVKDGILGGGGGGGLISGGGAEKLDLKEMIE 60
           MP+Y PS     DPKL+V CGA ILDL+TVVKDGILGGGGGG + + GGA+KLDLKEMIE
Sbjct: 1   MPIYHPSICTQRDPKLLVDCGAHILDLQTVVKDGILGGGGGGVIATDGGAQKLDLKEMIE 60

Query: 61  ELESSIEVPSVFICPISLEPMQDPVTLCSGQTYERSNILKWFSLGHLTCPTTMQELWDDS 120
           ELESSIEVPSVFICPISLEPMQDPVTLCSGQTYERSNILKWFSLGHLTCPTTMQELWDDS
Sbjct: 61  ELESSIEVPSVFICPISLEPMQDPVTLCSGQTYERSNILKWFSLGHLTCPTTMQELWDDS 120

Query: 121 VTPNRTLHQLIHTWFSQKYLALKKRSEDVQGRVLELLDSLKKVKGQARVQSLKDLRHVVA 180
           VTPNRTLHQLIHTWFSQKYLALKKRSEDVQGRVLELLDSLKKVKGQARVQSLKDLRHVV 
Sbjct: 121 VTPNRTLHQLIHTWFSQKYLALKKRSEDVQGRVLELLDSLKKVKGQARVQSLKDLRHVVT 180

Query: 181 THSSAKKMVVDNGGVALISSLLGSFTSHAVGSEAVGILVNLDLDSELKKNLIQPTRMSLI 240
            HSS KKMVVDNGGVALISSLLGSFTSHAVGSE VGILVNLDL+SELKKNLIQPTR+SLI
Sbjct: 181 AHSSVKKMVVDNGGVALISSLLGSFTSHAVGSEGVGILVNLDLNSELKKNLIQPTRISLI 240

Query: 241 ADILNEGSIETKINCTKFIKMLIEDDDLRTENVSSLSLLVGLLRMVKDQRHPNGVTAGLS 300
           ADILNEGSIETKINCTKFIKML E D    ENVSSLSLLVGLLRMVKDQRHPNGVTA L+
Sbjct: 241 ADILNEGSIETKINCTKFIKMLTEGDASTIENVSSLSLLVGLLRMVKDQRHPNGVTAALT 300

Query: 301 LLRTLCSNESIRKQLVSIGAVAKLGGALSGLNADCLESALYILDVLSSLPEGRLALKDCE 360
           LLRTLCSNESIRKQLV IGAVAKLGGALSGLNA+ LESALYILDVLSSLPEGRLALK+CE
Sbjct: 301 LLRTLCSNESIRKQLVGIGAVAKLGGALSGLNAESLESALYILDVLSSLPEGRLALKNCE 360

Query: 361 DTIRNLVKLLMKVSEICTQFALSILWAVFKLAPEECAVLAVEAGLAAKLLLVIQSGCNKE 420
           DT+RNLVKLLMKVSEICT+FALSILWAVFKLAPEEC+VLAVEAGLAAKLLLVIQSG N E
Sbjct: 361 DTVRNLVKLLMKVSEICTRFALSILWAVFKLAPEECSVLAVEAGLAAKLLLVIQSGFNPE 420

Query: 421 LKQRSSELLKLCSLNYPDSIFISKCKLTRTIQ 453
           LKQRS+ELLKLCSLNYPDSIFISKCKLTRTIQ
Sbjct: 421 LKQRSAELLKLCSLNYPDSIFISKCKLTRTIQ 452

BLAST of Lag0004204 vs. TAIR 10
Match: AT3G49810.1 (ARM repeat superfamily protein )

HSP 1 Score: 555.4 bits (1430), Expect = 4.1e-158
Identity = 291/452 (64.38%), Postives = 360/452 (79.65%), Query Frame = 0

Query: 1   MPMYQPSAWRDGDPKLVVGCGAQILDLETVVKDGILGGGGGGGLISGGGAEKLDLKEMIE 60
           MPM+QP   RDG      G   Q+LDL+T VKDG+LGG  GGG+  G   EKLDLK+MI+
Sbjct: 1   MPMFQPLK-RDGLIGFEGGGDGQVLDLDTAVKDGVLGGVNGGGV--GVVDEKLDLKKMIK 60

Query: 61  ELESSIEVPSVFICPISLEPMQDPVTLCSGQTYERSNILKWFSLGHLTCPTTMQELWDDS 120
           EL+   ++PSVFICPISLEPMQDPVTLC+GQTYER NI KWF+LGHLTCPTTMQELWDD+
Sbjct: 61  ELDLQ-DIPSVFICPISLEPMQDPVTLCTGQTYERLNIHKWFNLGHLTCPTTMQELWDDT 120

Query: 121 VTPNRTLHQLIHTWFSQKYLALKKRSEDVQGRVLELLDSLKKVKGQARVQSLKDLRHVVA 180
           VTPN+TLH LI+TWFSQKY+ +KKRSEDVQGR +E+L +LKK KGQARV +L +L+ +V 
Sbjct: 121 VTPNKTLHHLIYTWFSQKYVLMKKRSEDVQGRAIEILGTLKKAKGQARVHALSELKQIVI 180

Query: 181 THSSAKKMVVDNGGVALISSLLGSFTSHAVGSEAVGILVNLDLDSELKKNLIQPTRMSLI 240
            H  A+K VV+ GGV++ISSLLG FTSHAVGSE V ILV+LDLDS+ K  L+QP ++SLI
Sbjct: 181 AHLMARKTVVEEGGVSVISSLLGPFTSHAVGSEVVAILVSLDLDSDSKSGLMQPAKVSLI 240

Query: 241 ADILNEGSIETKINCTKFIKMLIEDDDLRTENVSSLSLLVGLLRMVKDQRHPNGVTAGLS 300
            D+LN+GS ETKINC + I+ L+E+   R E VSS SLLVGL+R+VKD+RH NGV+  L 
Sbjct: 241 VDMLNDGSNETKINCARLIRGLVEEKGFRAELVSSHSLLVGLMRLVKDKRHRNGVSPALR 300

Query: 301 LLRTLCSNESIRKQLVSIGAVAKLGGALSGLNADCLESALYILDVLSSLPEGRLALKDCE 360
           LL+ +  ++ +R  +VSIGAV +L   L  L+ +CLE AL++LD L +  EGR+A+KD  
Sbjct: 301 LLKPISVHKQVRSLMVSIGAVPQLVDILPSLDPECLELALFVLDALCTDVEGRVAVKDSA 360

Query: 361 DTIRNLVKLLMKVSEICTQFALSILWAVFKLAPEECAVLAVEAGLAAKLLLVIQSGCNKE 420
           +TI   V++LM+VSE CT +ALSILW+V KLAPEEC+ LAVE GLAAKLLLVIQSGC+  
Sbjct: 361 NTIPYTVRVLMRVSENCTNYALSILWSVCKLAPEECSPLAVEVGLAAKLLLVIQSGCDAA 420

Query: 421 LKQRSSELLKLCSLNYPDSIFISKCKLTRTIQ 453
           LKQRS+ELLKLCSL+Y D++FISKCKLTRTIQ
Sbjct: 421 LKQRSAELLKLCSLHYSDTMFISKCKLTRTIQ 448

BLAST of Lag0004204 vs. TAIR 10
Match: AT5G65920.1 (ARM repeat superfamily protein )

HSP 1 Score: 550.8 bits (1418), Expect = 1.0e-156
Identity = 293/453 (64.68%), Postives = 358/453 (79.03%), Query Frame = 0

Query: 1   MPMYQPSAWRDGDPKLVVGCGAQILDLETVVKDGILGGGGGGGL-ISGGGAEKLDLKEMI 60
           MPM+QPS  ++G      G    ILDL + VKDG+LGGG G  L +     +KLDLKEMI
Sbjct: 1   MPMFQPS--KNG------GFDGHILDLHSAVKDGVLGGGDGKFLVVVTDEKKKLDLKEMI 60

Query: 61  EELESSIEVPSVFICPISLEPMQDPVTLCSGQTYERSNILKWFSLGHLTCPTTMQELWDD 120
            ELE   E+PSVFICPISLEPMQDPVTLC+GQTYERSNILKWF++GH TCPTTMQELWDD
Sbjct: 61  SELELP-EIPSVFICPISLEPMQDPVTLCTGQTYERSNILKWFNIGHCTCPTTMQELWDD 120

Query: 121 SVTPNRTLHQLIHTWFSQKYLALKKRSEDVQGRVLELLDSLKKVKGQARVQSLKDLRHVV 180
            VTPN+TLHQLI+TWFSQKY+ +KKRSEDVQGR +E+L +L+K KG+A+V +L +L+ VV
Sbjct: 121 LVTPNKTLHQLIYTWFSQKYVLMKKRSEDVQGRAIEILGTLRKAKGKAKVHALSELKQVV 180

Query: 181 ATHSSAKKMVVDNGGVALISSLLGSFTSHAVGSEAVGILVNLDLDSELKKNLIQPTRMSL 240
             H+ AKK VVD GGV +ISSLL  FTSHAVGSEA+ ILVNL+LDS+ K  L+QP R+SL
Sbjct: 181 MAHAIAKKTVVDEGGVFVISSLLSPFTSHAVGSEAIAILVNLELDSDSKAGLMQPARVSL 240

Query: 241 IADILNEGSIETKINCTKFIKMLIEDDDLRTENVSSLSLLVGLLRMVKDQRHPNGVTAGL 300
           + D+LN+GSIETKINC + I  L+E+   R E VSS SLLVGL+R+VKD+R  NGV+  L
Sbjct: 241 MVDMLNDGSIETKINCARLIGRLVEEKGFRAELVSSHSLLVGLMRLVKDRRRRNGVSPAL 300

Query: 301 SLLRTLCSNESIRKQLVSIGAVAKLGGALSGLNADCLESALYILDVLSSLPEGRLALKDC 360
           +LL+++  ++ +R  LV IGAV +L   L  L+ +CLESAL++LD L    EGR+ALKD 
Sbjct: 301 TLLKSVSVHKQVRNLLVRIGAVPQLVDVLPCLDVECLESALFVLDSLCLESEGRIALKDS 360

Query: 361 EDTIRNLVKLLMKVSEICTQFALSILWAVFKLAPEECAVLAVEAGLAAKLLLVIQSGCNK 420
            +TI + V+LLMKVSE CT +A+SILW+V KLA EEC+ LAVE GLAAKLLLVIQSGC+ 
Sbjct: 361 VNTIPHTVRLLMKVSEKCTNYAISILWSVCKLASEECSSLAVEVGLAAKLLLVIQSGCDP 420

Query: 421 ELKQRSSELLKLCSLNYPDSIFISKCKLTRTIQ 453
            LKQRS+ELLKLCSL+Y DS+FISKCKLTRTIQ
Sbjct: 421 ALKQRSAELLKLCSLHYSDSMFISKCKLTRTIQ 444

BLAST of Lag0004204 vs. TAIR 10
Match: AT3G19380.1 (plant U-box 25 )

HSP 1 Score: 153.7 bits (387), Expect = 3.6e-37
Identity = 126/405 (31.11%), Postives = 203/405 (50.12%), Query Frame = 0

Query: 59  IEELESSIEVPSVFICPISLEPMQDPVTLCSGQTYERSNILKWFSLG-HLTCPTTMQELW 118
           IE L+  I++P  F CPISLE MQDPVT+C+GQTY+R++I  W S+G + TCP T   L 
Sbjct: 5   IEPLDLGIQIPYHFRCPISLELMQDPVTVCTGQTYDRASIESWVSIGNNTTCPVTRAPLS 64

Query: 119 DDSVTPNRTLHQLIHTWFSQKYLALKKRSEDVQ-----------GRVLELLDSLKKVKG- 178
           D ++ PN TL +LI  W       +  RS  V+             V  LL     + G 
Sbjct: 65  DFTLIPNHTLRRLIQEW------CVANRSNGVERIPTPKQPADPTSVRALLSQASAITGT 124

Query: 179 ----QARVQSLKDLRHVVATHSSAKKMV-VDNGGVALISSLLGSFTSHAVGSEAVGILVN 238
               ++R  +L+ LR         + ++   N    LI  L    TS  + SE++ +LV 
Sbjct: 125 HVSVRSRAAALRRLRGFARDSDKNRVLIAAHNATEILIKILFSETTSSELVSESLALLVM 184

Query: 239 LDL--DSELKKNLIQPTRMSLIADILNEGSIETKINCTKFIKML---IEDDDLRTENVSS 298
           L +   ++       P R+  +  +L + SIET++N    I+++    +  DL+    +S
Sbjct: 185 LPITEPNQFVSISSDPGRVEFLTRLLFDSSIETRVNAAALIEIVSTGTKSADLKGSISNS 244

Query: 299 LSLLVGLLRMVKDQ-RHPNGVTAGLSLLRTLCSNESIRKQLVSIGAVAKLGGALSGLNAD 358
            S+  G+L ++++       +  G+  L  LCS +S R   ++ GA   L   L+     
Sbjct: 245 ESVFEGVLDLLRNPISSRRALKIGIKTLFALCSVKSTRHIAITAGAPEILIDRLAADFDR 304

Query: 359 C-LESALYILDVLSSLPEGRLALKDCEDTIRNLVKLLMKVSEICTQFALSILWAVFKLAP 418
           C  E AL  +++L   PEG  A  +   T+  LVK +++VS+  T++A   L A+   A 
Sbjct: 305 CDTERALATVELLCRTPEGCAAFGEHALTVPLLVKTILRVSDRATEYAAGALLALC-TAE 364

Query: 419 EECAVLAVEAGLAAKLLLVIQSGCNKELKQRSSELLKLCSLNYPD 439
           E     A  AG+  +LLL++QS C +  K+++ +LLKL   ++PD
Sbjct: 365 ERWREEAAGAGVVVQLLLMVQSECTERAKKKAQKLLKLLRDSWPD 402

BLAST of Lag0004204 vs. TAIR 10
Match: AT1G49780.1 (plant U-box 26 )

HSP 1 Score: 149.1 bits (375), Expect = 8.8e-36
Identity = 119/408 (29.17%), Postives = 204/408 (50.00%), Query Frame = 0

Query: 59  IEELESSIEVPSVFICPISLEPMQDPVTLCSGQTYERSNILKWFSLGHLTCPTTMQELWD 118
           +E L+  I++P  F CPISL+ M DPVT+ +GQTY+R++I  W ++G+ TCP T   L D
Sbjct: 5   LEPLDLGIQIPYHFRCPISLDLMSDPVTISTGQTYDRTSIDSWIAMGNTTCPVTRVALSD 64

Query: 119 DSVTPNRTLHQLIHTWFSQKYLALKKRSEDVQG-----------RVLELLDSLKKVKG-- 178
            ++ PN TL +LI  W       +  RS  V+             V  LL     + G  
Sbjct: 65  FTLIPNHTLRRLIQEW------CVANRSNGVERIPTPKQPADPISVRSLLSQASAITGTH 124

Query: 179 ---QARVQSLKDLRHVVATHSSAKKMVVDNGGVALISSLL------GSFTSHAVGSEAVG 238
              ++R  +++ LR +       + ++  +    ++  +L       S +S  V SE++ 
Sbjct: 125 VSVRSRAAAIRRLRGLARDSEKNRVLIAGHNAREILVRILFADIETTSLSSELV-SESLA 184

Query: 239 ILVNLDL-DSELKKNLIQPTRMSLIADILNEGSIETKINCTKFIKMLI---EDDDLRTEN 298
           +LV L + ++E +     P+R+  +  +L + SIE ++N    I+M++   +  DL+   
Sbjct: 185 LLVLLHMTETECEAVASDPSRVGFMTRLLFDSSIEIRVNAAALIEMVLTGAKSMDLKLII 244

Query: 299 VSSLSLLVGLLRMVKDQ-RHPNGVTAGLSLLRTLCSNESIRKQLVSIGAVAKLGGALSGL 358
             S S+  G+L ++K+       +  G+  +  LC  +  R   +S GA   L   L+  
Sbjct: 245 SGSDSIFEGVLDLLKNPISSRRALKIGIKAIFALCLVKQTRHLAISAGAPGILIDRLAAD 304

Query: 359 NADC-LESALYILDVLSSLPEGRLALKDCEDTIRNLVKLLMKVSEICTQFALSILWAVFK 418
              C  E  L  +++L  LPEG  A  +   T+  +VK +++VS+  T++A   L A+  
Sbjct: 305 FDRCDTERGLATVELLCRLPEGCAAFGEHALTVPLMVKTILRVSDRATEYAAGALLALC- 364

Query: 419 LAPEECAVLAVEAGLAAKLLLVIQSGCNKELKQRSSELLKLCSLNYPD 439
            A E C   A  AGL  +LLL++QS C +  K+++  LLKL   ++PD
Sbjct: 365 TAEERCRDEAAAAGLVTQLLLLVQSDCTERAKRKAQMLLKLLRDSWPD 404

BLAST of Lag0004204 vs. TAIR 10
Match: AT5G64660.1 (CYS, MET, PRO, and GLY protein 2 )

HSP 1 Score: 143.7 bits (361), Expect = 3.7e-34
Identity = 122/398 (30.65%), Postives = 203/398 (51.01%), Query Frame = 0

Query: 66  IEVPSVFICPISLEPMQDPVTLCSGQTYERSNILKWFSLGHLTCPTTMQELWDDSVTPNR 125
           I VP+ F CPISL+ M+ PV+LC+G TY+R++I +W   G+ TCP TMQ L +    PNR
Sbjct: 8   ITVPTFFRCPISLDVMKSPVSLCTGVTYDRASIQRWLDGGNNTCPATMQILQNKDFIPNR 67

Query: 126 TLHQLIHTWF-SQKYLALKKRSEDVQGRVLELLDSLKKVKGQARVQSLKDLRHVVATHSS 185
           TL +LI  W  S +     + +E       E+ D++ +VK +   +  +D R V++    
Sbjct: 68  TLQRLIEIWSDSVRRRTCVESAELAAPTRDEIADAIDRVKIE---KEERDDREVLSKIVR 127

Query: 186 AKKMVVDNGG------------VALISSLLGSFTSHA---VGSEAVGILVNLD---LDSE 245
             +   DN G            V LI+ +    TS A   V  EAV IL  +     D  
Sbjct: 128 FGRESDDNRGFLAGKDDFVKLLVDLINQVDFETTSAAKSLVVQEAVKILSTIRSKVSDRR 187

Query: 246 LKKNLIQPT---RMSLIADILNEGSIETKINCTKFIKMLIEDDDLRTENVSSLSLLVGLL 305
              NLI      R+S+I  +   G++E KI+C   ++ +  D + +        L+  L+
Sbjct: 188 RFSNLILTNGRDRLSVIVYLFKTGNVELKIDCAGLLEFIAVDAESKLLIAERDGLITELM 247

Query: 306 RMVKDQRHPNGVTAGLSLLRTLCSNESIRKQLVS---IGAVAK-LGGALSGLNADCLESA 365
           + +      + + + LS L  + S + ++  L+    IG V K L  + S L+    E  
Sbjct: 248 KSISKDSDLSLIESSLSCLIAISSPKRVKLNLLREKLIGDVTKLLSDSTSSLSVSVTEKC 307

Query: 366 LYILDVLSSLPEGRLALKDCE-DTIRNLVKLLMKVSEICTQFALSILWAVFKLAPEECAV 425
           L +L++L+S  EGR  +   + + ++ +VK LMKVS   T+ A+++LW+V  L  E+ A+
Sbjct: 308 LKLLEILASTKEGRSEICGGDGECLKTVVKKLMKVSTAATEHAVTVLWSVSYLFKEDKAL 367

Query: 426 LAV-EAGLAAKLLLVIQSGCNKELKQRSSELLKLCSLN 436
            AV       K+LL++QS C+  +++  ++LLK+  +N
Sbjct: 368 EAVTSVNGVTKILLLLQSNCSPAVRRMLTDLLKVFKVN 402