Lag0004138 (gene) Sponge gourd (AG‐4) v1

Overview


Name	Lag0004138
Type	gene
Organism	Luffa acutangula (Sponge gourd (AG‐4) v1)
Description	WRKY domain-containing protein
Location	chr6: 1373820 .. 1374895 (+)
RNA-Seq Expression	Lag0004138
Synteny	Lag0004138

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonCDSpolypeptide

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGTCTTACAACAGCAACCAGAACCTCGCTTCGTCGGAGGGCGATCTTGCCGAGCAGCCCGGCTTCGAATTTACAGACTGGATGTTTGATGGATGGCTCAATGAAAACTCTTCGTCGCCTCTGGCCGACTCGGTGATGTACCCGGTTTATCAATCGGGCGAGGTTGCTGATGAGTTTGTTGGGAACACCATCCCCCAAGGAGAGCCTAGCAGTAGTAAGCACATAACTCTCATTACATTTTAGCATTTGTAAGATTTTGATGTTTTTGTTTTGTTTGAGGAACAAACTTCTTTCTCGCTAACCACGACTAAAAACCTTATTGCTATTCATTTTCTGAAAAGAAAAGAGTCAAAAGTATCTCTTTCCTATCTCCCTTTCCGTATTTCTCTCTTCTACCTGTCAAAAACTTAACAGTAGTGACCAATCTGTAGATAAGTATTATCTTTTTCTTAGAAAAGTAAATAGCGGTAATATTTTTAGTGGTGGTTAACGGGAATGATTCCTCCTGATCAACCAGGAGAGAAGTATCTCTTTTCTCTTAGTCTTTTTCTATATTCCTCTCTATTTTTATAGTGGTGACCAATCAGTTGCAAGTATTATCTTACTACTGAGGTTGTGTTCTCATGAATGATCTTTAATGATGTTAGTAGGAGACATTGGGAATAATGGGAGAGAGAGGGAAGTTAGAGAAAGATTTGCATTCAAGACAAAATCAGAAGTTGAGATTCTGGATGATGGGTTCAAGTGGAGGAAGTATGGGAAGAAGATGGTGAAAAACAGCCCAAATCCAAGGTAAGAAAATAAAAGAACCAGTAATAATGTTCCTAATTGAACATGTAATTCTGTCCTAAAATAACATGTTTCATTGTTAGAAACAGCAGGGATGAACTAGAACCCAATGAACAAGTTGTGTGTGTTGGTAATCTGAATTGGCTCAATCATCTATGAAATGCAGGAACTACTACAAATGCTCGGTCGAAGGCTGCCCAGTGAAGAAGAGAGTCGAAAGAGATCGAGAGGATCCGAAATATGTGATTACGACGTACGAGGGTGTTCATACCCATGAAAGTTCTTGA

mRNA sequence

ATGTCTTACAACAGCAACCAGAACCTCGCTTCGTCGGAGGGCGATCTTGCCGAGCAGCCCGGCTTCGAATTTACAGACTGGATGTTTGATGGATGGCTCAATGAAAACTCTTCGTCGCCTCTGGCCGACTCGGTGATGTACCCGGTTTATCAATCGGGCGAGGTTGCTGATGAGTTTGTTGGGAACACCATCCCCCAAGGAGAGCCTAGCAGTAGAGACATTGGGAATAATGGGAGAGAGAGGGAAGTTAGAGAAAGATTTGCATTCAAGACAAAATCAGAAGTTGAGATTCTGGATGATGGGTTCAAGTGGAGGAAGTATGGGAAGAAGATGGTGAAAAACAGCCCAAATCCAAGGAACTACTACAAATGCTCGGTCGAAGGCTGCCCAGTGAAGAAGAGAGTCGAAAGAGATCGAGAGGATCCGAAATATGTGATTACGACGTACGAGGGTGTTCATACCCATGAAAGTTCTTGA

Coding sequence (CDS)

ATGTCTTACAACAGCAACCAGAACCTCGCTTCGTCGGAGGGCGATCTTGCCGAGCAGCCCGGCTTCGAATTTACAGACTGGATGTTTGATGGATGGCTCAATGAAAACTCTTCGTCGCCTCTGGCCGACTCGGTGATGTACCCGGTTTATCAATCGGGCGAGGTTGCTGATGAGTTTGTTGGGAACACCATCCCCCAAGGAGAGCCTAGCAGTAGAGACATTGGGAATAATGGGAGAGAGAGGGAAGTTAGAGAAAGATTTGCATTCAAGACAAAATCAGAAGTTGAGATTCTGGATGATGGGTTCAAGTGGAGGAAGTATGGGAAGAAGATGGTGAAAAACAGCCCAAATCCAAGGAACTACTACAAATGCTCGGTCGAAGGCTGCCCAGTGAAGAAGAGAGTCGAAAGAGATCGAGAGGATCCGAAATATGTGATTACGACGTACGAGGGTGTTCATACCCATGAAAGTTCTTGA

Protein sequence

MSYNSNQNLASSEGDLAEQPGFEFTDWMFDGWLNENSSSPLADSVMYPVYQSGEVADEFVGNTIPQGEPSSRDIGNNGREREVRERFAFKTKSEVEILDDGFKWRKYGKKMVKNSPNPRNYYKCSVEGCPVKKRVERDREDPKYVITTYEGVHTHESS

Homology

[Close]

BLAST of Lag0004138 vs. NCBI nr
Match: XP_004144271.1 (probable WRKY transcription factor 50 [Cucumis sativus])

HSP 1 Score: 288.1 bits (736), Expect = 4.6e-74
Identity = 143/158 (90.51%), Postives = 147/158 (93.04%), Query Frame = 0


Query: 1   MSYNSNQNLASSEGDLAEQPGFEFTDWMFDGWLNENSSSPLADSVMYPVYQSGEVADEFV 60
           MSYNSNQ+L +SE DL+EQPGFEFTDWMFDGWLNENSSS L DSVMYPVYQ GEV DEFV
Sbjct: 1   MSYNSNQHLTTSESDLSEQPGFEFTDWMFDGWLNENSSS-LTDSVMYPVYQEGEV-DEFV 60

Query: 61  GNTIPQGEPSSRDIGNNGREREVRERFAFKTKSEVEILDDGFKWRKYGKKMVKNSPNPRN 120
           GNTI QGEPSSRD    GRERE+RERFAFKTKSEVEILDDGFKWRKYGKKMVKNSPNPRN
Sbjct: 61  GNTIQQGEPSSRDY---GREREIRERFAFKTKSEVEILDDGFKWRKYGKKMVKNSPNPRN 120

Query: 121 YYKCSVEGCPVKKRVERDREDPKYVITTYEGVHTHESS 159
           YYKCSVEGCPVKKRVERDREDPKYVITTYEGVHTHESS
Sbjct: 121 YYKCSVEGCPVKKRVERDREDPKYVITTYEGVHTHESS 153

[Close]

BLAST of Lag0004138 vs. NCBI nr
Match: XP_008445519.1 (PREDICTED: probable WRKY transcription factor 50 isoform X1 [Cucumis melo] >KAA0064721.1 putative WRKY transcription factor 50 isoform X1 [Cucumis melo var. makuwa])

HSP 1 Score: 285.4 bits (729), Expect = 3.0e-73
Identity = 142/158 (89.87%), Postives = 147/158 (93.04%), Query Frame = 0


Query: 1   MSYNSNQNLASSEGDLAEQPGFEFTDWMFDGWLNENSSSPLADSVMYPVYQSGEVADEFV 60
           MSYNSNQ+L +SE DL+EQPGFEFTDWMFDGWLNENSS+ L DSVMYPVYQ GEV DEFV
Sbjct: 1   MSYNSNQHLTTSESDLSEQPGFEFTDWMFDGWLNENSST-LTDSVMYPVYQEGEV-DEFV 60

Query: 61  GNTIPQGEPSSRDIGNNGREREVRERFAFKTKSEVEILDDGFKWRKYGKKMVKNSPNPRN 120
           GNTI Q EPSSRD   +GREREVRERFAFKTKSEVEILDDGFKWRKYGKKMVKNSPNPRN
Sbjct: 61  GNTIQQAEPSSRD---HGREREVRERFAFKTKSEVEILDDGFKWRKYGKKMVKNSPNPRN 120

Query: 121 YYKCSVEGCPVKKRVERDREDPKYVITTYEGVHTHESS 159
           YYKCSVEGCPVKKRVERDREDPKYVITTYEGVHTHESS
Sbjct: 121 YYKCSVEGCPVKKRVERDREDPKYVITTYEGVHTHESS 153

[Close]

BLAST of Lag0004138 vs. NCBI nr
Match: XP_022131756.1 (probable WRKY transcription factor 50 [Momordica charantia])

HSP 1 Score: 280.8 bits (717), Expect = 7.3e-72
Identity = 139/159 (87.42%), Postives = 147/159 (92.45%), Query Frame = 0


Query: 1   MSYNSNQNLASSEGDLAEQPGFEFTDWMFDGWLNENSSSPLADSVMYPVYQSGEVADEFV 60
           MSYNSNQ+LA+SE DLAEQPGFEF DWMFDGWLNENSSS LA+SVMYPVY++GE  DE V
Sbjct: 1   MSYNSNQHLATSESDLAEQPGFEFPDWMFDGWLNENSSS-LAESVMYPVYKAGEEVDEIV 60

Query: 61  G-NTIPQGEPSSRDIGNNGREREVRERFAFKTKSEVEILDDGFKWRKYGKKMVKNSPNPR 120
           G NTI QGEPSSRD    GREREVRERFAFKTKSE+EILDDGFKWRKYGKKMVKNSPNPR
Sbjct: 61  GINTIHQGEPSSRD---RGREREVRERFAFKTKSEIEILDDGFKWRKYGKKMVKNSPNPR 120

Query: 121 NYYKCSVEGCPVKKRVERDREDPKYVITTYEGVHTHESS 159
           NYYKCS+EGCPVKKRVERDREDP+YVITTYEGVHTHESS
Sbjct: 121 NYYKCSIEGCPVKKRVERDREDPRYVITTYEGVHTHESS 155

[Close]

BLAST of Lag0004138 vs. NCBI nr
Match: TYK00741.1 (putative WRKY transcription factor 50 isoform X1 [Cucumis melo var. makuwa])

HSP 1 Score: 279.3 bits (713), Expect = 2.1e-71
Identity = 142/176 (80.68%), Postives = 148/176 (84.09%), Query Frame = 0


Query: 1   MSYNSNQNLASSEGDLAEQPGFEFTDWMFDGWLNENSSSPLADSVMYPVYQSGEVADEFV 60
           MSYNSNQ+L +SE DL+EQPGFEFTDWMFDGWLNENSS+ L DSVMYPVYQ GEV DEFV
Sbjct: 1   MSYNSNQHLTTSESDLSEQPGFEFTDWMFDGWLNENSST-LTDSVMYPVYQEGEV-DEFV 60

Query: 61  GNTIPQGEPS------------------SRDIGNNGREREVRERFAFKTKSEVEILDDGF 120
           GNTI Q EPS                  SR  G++GREREVRERFAFKTKSEVEILDDGF
Sbjct: 61  GNTIQQAEPSSSGVFSPATIKDCTAFYISRSSGDHGREREVRERFAFKTKSEVEILDDGF 120

Query: 121 KWRKYGKKMVKNSPNPRNYYKCSVEGCPVKKRVERDREDPKYVITTYEGVHTHESS 159
           KWRKYGKKMVKNSPNPRNYYKCSVEGCPVKKRVERDREDPKYVITTYEGVHTHESS
Sbjct: 121 KWRKYGKKMVKNSPNPRNYYKCSVEGCPVKKRVERDREDPKYVITTYEGVHTHESS 174

[Close]

BLAST of Lag0004138 vs. NCBI nr
Match: XP_023546261.1 (probable WRKY transcription factor 50 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 277.3 bits (708), Expect = 8.0e-71
Identity = 137/158 (86.71%), Postives = 144/158 (91.14%), Query Frame = 0


Query: 1   MSYNSNQNLASSEGDLAEQPGFEFTDWMFDGWLNENSSSPLADSVMYPVYQSGEVADEFV 60
           MSYNSNQ+L  SE DL EQPGFEFTDWMFD WL+EN S PL DS MYPVYQSGE+ +EFV
Sbjct: 1   MSYNSNQHLVMSENDLPEQPGFEFTDWMFDRWLDEN-SPPLVDSAMYPVYQSGEI-EEFV 60

Query: 61  GNTIPQGEPSSRDIGNNGREREVRERFAFKTKSEVEILDDGFKWRKYGKKMVKNSPNPRN 120
           G+TI QGEPSSRD   +GREREVRERFAFKTKSEVE+LDDGFKWRKYGKKMVKNSPNPRN
Sbjct: 61  GSTIQQGEPSSRD---SGREREVRERFAFKTKSEVEVLDDGFKWRKYGKKMVKNSPNPRN 120

Query: 121 YYKCSVEGCPVKKRVERDREDPKYVITTYEGVHTHESS 159
           YYKCSVEGCPVKKRVERDREDPKYVITTYEGVHTHESS
Sbjct: 121 YYKCSVEGCPVKKRVERDREDPKYVITTYEGVHTHESS 153

[Close]

BLAST of Lag0004138 vs. ExPASy Swiss-Prot
Match: Q8VWQ5 (Probable WRKY transcription factor 50 OS=Arabidopsis thaliana OX=3702 GN=WRKY50 PE=2 SV=1)

HSP 1 Score: 147.1 bits (370), Expect = 1.6e-34
Identity = 82/170 (48.24%), Postives = 109/170 (64.12%), Query Frame = 0


Query: 4   NSNQNLASSEGDLAEQPGFEFTDW-MFDGWLNENSSSPLADSVMYPVYQSGEVADE-FVG 63
           +++ NL SS  D      FEF +  + D W++++  S ++       YQ+ +VA   F G
Sbjct: 3   DADTNLGSSFSDDTHSV-FEFPELDLSDEWMDDDLVSAVSGMNQSYGYQTSDVAGALFSG 62

Query: 64  NTIPQGEP--------------SSRDIGNNGREREVRERFAFKTKSEVEILDDGFKWRKY 123
           ++     P              +S D  N   +++++ R AFKT+SEVE+LDDGFKWRKY
Sbjct: 63  SSSCFSHPESPSTKTYVAATATASADNQNKKEKKKIKGRVAFKTRSEVEVLDDGFKWRKY 122

Query: 124 GKKMVKNSPNPRNYYKCSVEGCPVKKRVERDREDPKYVITTYEGVHTHES 158
           GKKMVKNSP+PRNYYKCSV+GCPVKKRVERDR+DP +VITTYEG H H S
Sbjct: 123 GKKMVKNSPHPRNYYKCSVDGCPVKKRVERDRDDPSFVITTYEGSHNHSS 171

[Close]

BLAST of Lag0004138 vs. ExPASy Swiss-Prot
Match: Q93WU9 (Probable WRKY transcription factor 51 OS=Arabidopsis thaliana OX=3702 GN=WRKY51 PE=1 SV=1)

HSP 1 Score: 130.6 bits (327), Expect = 1.6e-29
Identity = 70/159 (44.03%), Postives = 98/159 (61.64%), Query Frame = 0


Query: 2   SYNSNQNLAS---SEGDLAEQPGFEFTDWMFDGWLNENSSSPLADSVMYPVYQSGEVADE 61
           +Y S++N  +      D +    F+  +   +G + E  SSP   S++     +GE    
Sbjct: 12  TYFSDENFINPFMDNNDFSNLMFFDIDEGGNNGLIEEEISSP--TSIVSSETFTGESGGS 71

Query: 62  FVGNTIPQGEPSSRDIGNNGREREVRERFAFKTKSEVEILDDGFKWRKYGKKMVKNSPNP 121
               T+ + E ++R    + + +E   R AF+T+S+++++DDGFKWRKYGKK VKN+ N 
Sbjct: 72  GSATTLSKKESTNRGSKESDQTKETGHRVAFRTRSKIDVMDDGFKWRKYGKKSVKNNINK 131

Query: 122 RNYYKCSVEGCPVKKRVERDREDPKYVITTYEGVHTHES 158
           RNYYKCS EGC VKKRVERD +D  YVITTYEGVH HES
Sbjct: 132 RNYYKCSSEGCSVKKRVERDGDDAAYVITTYEGVHNHES 168

[Close]

BLAST of Lag0004138 vs. ExPASy Swiss-Prot
Match: Q9SVB7 (Probable WRKY transcription factor 13 OS=Arabidopsis thaliana OX=3702 GN=WRKY13 PE=1 SV=1)

HSP 1 Score: 109.8 bits (273), Expect = 2.9e-23
Identity = 55/98 (56.12%), Postives = 71/98 (72.45%), Query Frame = 0


Query: 62  NTIPQGEPSSRDIGNNGREREVRE-RFAFKTKSEVEILDDGFKWRKYGKKMVKNSPNPRN 121
           NT   G  SS  +      R+VRE RF FKT SEV++LDDG++WRKYG+K+VKN+ +PR+
Sbjct: 185 NTASLGVVSSLKMKKLKTRRKVREPRFCFKTLSEVDVLDDGYRWRKYGQKVVKNTQHPRS 244

Query: 122 YYKCSVEGCPVKKRVERDREDPKYVITTYEGVHTHESS 159
           YY+C+ + C VKKRVER  +DP+ VITTYEG H H  S
Sbjct: 245 YYRCTQDKCRVKKRVERLADDPRMVITTYEGRHLHSPS 282

[Close]

BLAST of Lag0004138 vs. ExPASy Swiss-Prot
Match: Q9FGZ4 (Probable WRKY transcription factor 48 OS=Arabidopsis thaliana OX=3702 GN=WRKY48 PE=2 SV=1)

HSP 1 Score: 109.8 bits (273), Expect = 2.9e-23
Identity = 49/77 (63.64%), Postives = 63/77 (81.82%), Query Frame = 0


Query: 80  EREVRE-RFAFKTKSEVEILDDGFKWRKYGKKMVKNSPNPRNYYKCSVEGCPVKKRVERD 139
           +++ RE RFAF TKS+++ LDDG++WRKYG+K VKNSP PR+YY+C+  GC VKKRVER 
Sbjct: 201 QKKAREARFAFLTKSDIDNLDDGYRWRKYGQKAVKNSPYPRSYYRCTTVGCGVKKRVERS 260

Query: 140 REDPKYVITTYEGVHTH 156
            +DP  V+TTYEG HTH
Sbjct: 261 SDDPSIVMTTYEGQHTH 277

[Close]

BLAST of Lag0004138 vs. ExPASy Swiss-Prot
Match: Q93WV4 (WRKY transcription factor 71 OS=Arabidopsis thaliana OX=3702 GN=WRKY71 PE=2 SV=1)

HSP 1 Score: 107.8 bits (268), Expect = 1.1e-22
Identity = 61/136 (44.85%), Postives = 86/136 (63.24%), Query Frame = 0


Query: 32  WLNENSSSPLADSVM---YPVYQSGEVADEFVGNTIPQGE-----PSSRDIGNNGR---E 91
           +LN  S+SP+  S      P   + + +D+   N   +G+      SS+ +   G+   E
Sbjct: 60  YLNLTSNSPVVSSSSNEGEPKENTNDKSDQMEDN---EGDLHGVGESSKQLTKQGKKKGE 119

Query: 92  REVRE-RFAFKTKSEVEILDDGFKWRKYGKKMVKNSPNPRNYYKCSVEGCPVKKRVERDR 151
           ++ RE R AF TKSE++ L+DG++WRKYG+K VKNSP PR+YY+C+ + C VKKRVER  
Sbjct: 120 KKEREVRVAFMTKSEIDHLEDGYRWRKYGQKAVKNSPYPRSYYRCTTQKCNVKKRVERSF 179

Query: 152 EDPKYVITTYEGVHTH 156
           +DP  VITTYEG H H
Sbjct: 180 QDPSIVITTYEGKHNH 192

The following BLAST results are available for this feature:

BLAST of Lag0004138 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_004144271.1	4.6e-74	90.51	probable WRKY transcription factor 50 [Cucumis sativus]	[more]
XP_008445519.1	3.0e-73	89.87	PREDICTED: probable WRKY transcription factor 50 isoform X1 [Cucumis melo] >KAA0...	[more]
XP_022131756.1	7.3e-72	87.42	probable WRKY transcription factor 50 [Momordica charantia]	[more]
TYK00741.1	2.1e-71	80.68	putative WRKY transcription factor 50 isoform X1 [Cucumis melo var. makuwa]	[more]
XP_023546261.1	8.0e-71	86.71	probable WRKY transcription factor 50 [Cucurbita pepo subsp. pepo]	[more]

BLAST of Lag0004138 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
Q8VWQ5	1.6e-34	48.24	Probable WRKY transcription factor 50 OS=Arabidopsis thaliana OX=3702 GN=WRKY50 ...	[more]
Q93WU9	1.6e-29	44.03	Probable WRKY transcription factor 51 OS=Arabidopsis thaliana OX=3702 GN=WRKY51 ...	[more]
Q9SVB7	2.9e-23	56.12	Probable WRKY transcription factor 13 OS=Arabidopsis thaliana OX=3702 GN=WRKY13 ...	[more]
Q9FGZ4	2.9e-23	63.64	Probable WRKY transcription factor 48 OS=Arabidopsis thaliana OX=3702 GN=WRKY48 ...	[more]
Q93WV4	1.1e-22	44.85	WRKY transcription factor 71 OS=Arabidopsis thaliana OX=3702 GN=WRKY71 PE=2 SV=1	[more]

InterPro

Analysis Name: InterPro Annotations of Sponge gourd (AG-4) v1
Date Performed: 2022-08-01

Position :

Zoom :

x 1


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR003657	WRKY domain	SMART	SM00774	WRKY_cls	coord: 98..157 e-value: 4.3E-37 score: 139.2
IPR003657	WRKY domain	PFAM	PF03106	WRKY	coord: 99..156 e-value: 4.3E-25 score: 87.7
IPR003657	WRKY domain	PROSITE	PS50811	WRKY	coord: 93..158 score: 30.503548
IPR036576	WRKY domain superfamily	GENE3D	2.20.25.80	WRKY domain	coord: 85..151 e-value: 9.6E-33 score: 113.7
IPR036576	WRKY domain superfamily	SUPERFAMILY	118290	WRKY DNA-binding domain	coord: 91..156
IPR044810	WRKY transcription factor, plant	PANTHER	PTHR31221	WRKY TRANSCRIPTION FACTOR PROTEIN 1-RELATED	coord: 68..157
None	No IPR available	PANTHER	PTHR31221:SF112	WRKY TRANSCRIPTION FACTOR 59-RELATED	coord: 68..157

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
Lag0004138.1	Lag0004138.1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0006468	protein phosphorylation
biological_process	GO:0006355	regulation of transcription, DNA-templated
cellular_component	GO:0005634	nucleus
molecular_function	GO:0005524	ATP binding
molecular_function	GO:0003700	DNA-binding transcription factor activity
molecular_function	GO:0004674	protein serine/threonine kinase activity
molecular_function	GO:0043565	sequence-specific DNA binding