Lag0004094 (gene) Sponge gourd (AG‐4) v1

BLAST of Lag0004094 vs. NCBI nr
Match: XP_023522626.1 (probable carboxylesterase 18 isoform X1 [Cucurbita pepo subsp. pepo] >XP_023547293.1 probable carboxylesterase 18 isoform X1 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 634.4 bits (1635), Expect = 5.5e-178
Identity = 301/336 (89.58%), Postives = 316/336 (94.05%), Query Frame = 0

Query: 1   MSEALSALRLPWKVKMMLTAIAVGTDICCRSDGSVNRYLSNLLDFKSPPLKKPKNGLKSF 60
           MS+ALSALRLPWKVKMMLTAIAVGTDICCRSD SVNR+L+NL+DFKSPPLKKPKNG+KSF
Sbjct: 1   MSDALSALRLPWKVKMMLTAIAVGTDICCRSDVSVNRFLANLMDFKSPPLKKPKNGVKSF 60

Query: 61  DVTVDASRNLWFRLYTSTSDSSSGSLPLIVYFHGGGFVYMAPDSKLLDDFCQSLAREISA 120
           DVTVDASRNLWFRLY  TS+S+S SLPLIVYFHGGGFVYMAPDSKLLDDFCQSLAREI A
Sbjct: 61  DVTVDASRNLWFRLYAPTSESTSESLPLIVYFHGGGFVYMAPDSKLLDDFCQSLAREIHA 120

Query: 121 VVISVNYRLAPEYRYPCQYEDGFDVLKFIDGGGSAFEGFPANVDLKRCFLAGDSAGGNIA 180
           VVISVNYRLAPE+R PCQYEDGFDVLKFIDG GS+FEG PANVDLKRCF+AGDSAGGNIA
Sbjct: 121 VVISVNYRLAPEHRCPCQYEDGFDVLKFIDGDGSSFEGLPANVDLKRCFVAGDSAGGNIA 180

Query: 181 HHTILKSAEHEFRELEIIGLIAIQPFFGGEERTESEKELIKAPLSTMDRTDWYWKAFLPE 240
           HH ILKS +HEFRELEIIGLIAIQPFFGGEERTES+  LIKAPLST+DRTDWYWKAFLPE
Sbjct: 181 HHMILKSGDHEFRELEIIGLIAIQPFFGGEERTESDIRLIKAPLSTIDRTDWYWKAFLPE 240

Query: 241 GSDRDHPAVNIFGPNSVDISKVRFPAIKVFVGGLDPLIDWQKRYYEGLKKSGKEAYLTEY 300
           G DRDHP+VNIFGP SVDISKVRFPAIKV VGGLDPLIDWQKRYYEGLKKSGKEAYLTEY
Sbjct: 241 GFDRDHPSVNIFGPKSVDISKVRFPAIKVLVGGLDPLIDWQKRYYEGLKKSGKEAYLTEY 300

Query: 301 PNAFHSFYGFPELPEWPLFIKDAADFVRTQCSKKSS 337
           PNAFHSFYGFPELPEW  FIKD ADF+  QCSKKSS
Sbjct: 301 PNAFHSFYGFPELPEWSEFIKDVADFIAAQCSKKSS 336

BLAST of Lag0004094 vs. NCBI nr
Match: XP_022999791.1 (probable carboxylesterase 18 [Cucurbita maxima])

HSP 1 Score: 625.9 bits (1613), Expect = 2.0e-175
Identity = 297/336 (88.39%), Postives = 310/336 (92.26%), Query Frame = 0

Query: 1   MSEALSALRLPWKVKMMLTAIAVGTDICCRSDGSVNRYLSNLLDFKSPPLKKPKNGLKSF 60
           MS+ALSALRLPWKVKMMLTAIAVGTDICCR D SVNR+L+NL+DFKSPPLKKPKNG+KSF
Sbjct: 1   MSDALSALRLPWKVKMMLTAIAVGTDICCRRDVSVNRFLANLMDFKSPPLKKPKNGVKSF 60

Query: 61  DVTVDASRNLWFRLYTSTSDSSSGSLPLIVYFHGGGFVYMAPDSKLLDDFCQSLAREISA 120
           DVTVD SRNLWFRLY  TS+S S SLPLI YFHGGGFVYMAPDSKLLDDFCQSLAREI A
Sbjct: 61  DVTVDVSRNLWFRLYAPTSESMSESLPLIFYFHGGGFVYMAPDSKLLDDFCQSLAREIHA 120

Query: 121 VVISVNYRLAPEYRYPCQYEDGFDVLKFIDGGGSAFEGFPANVDLKRCFLAGDSAGGNIA 180
           VVISVNYRLAPE+  PCQYED FDVLKFIDG GS+FEG PANVDLKRCF+AGDSAGGNIA
Sbjct: 121 VVISVNYRLAPEHHCPCQYEDSFDVLKFIDGDGSSFEGLPANVDLKRCFVAGDSAGGNIA 180

Query: 181 HHTILKSAEHEFRELEIIGLIAIQPFFGGEERTESEKELIKAPLSTMDRTDWYWKAFLPE 240
           HH ILKS EH FRELEIIGLIAIQPFFGGEERTESE  L+KAPLST+DRTDWYWKAFLPE
Sbjct: 181 HHMILKSGEHGFRELEIIGLIAIQPFFGGEERTESEIRLLKAPLSTIDRTDWYWKAFLPE 240

Query: 241 GSDRDHPAVNIFGPNSVDISKVRFPAIKVFVGGLDPLIDWQKRYYEGLKKSGKEAYLTEY 300
           G DRDHP+VNIFGP SVDISKVRFPAIKV VGGLDPLIDWQKRYYEGLKKSGKEAYLTEY
Sbjct: 241 GFDRDHPSVNIFGPKSVDISKVRFPAIKVLVGGLDPLIDWQKRYYEGLKKSGKEAYLTEY 300

Query: 301 PNAFHSFYGFPELPEWPLFIKDAADFVRTQCSKKSS 337
           PNAFHSFYGFPELPEWP FIKD ADF+  QCSKKSS
Sbjct: 301 PNAFHSFYGFPELPEWPEFIKDVADFIAAQCSKKSS 336

BLAST of Lag0004094 vs. NCBI nr
Match: XP_038885745.1 (probable carboxylesterase 18 [Benincasa hispida])

HSP 1 Score: 624.0 bits (1608), Expect = 7.5e-175
Identity = 296/336 (88.10%), Postives = 311/336 (92.56%), Query Frame = 0

Query: 1   MSEALSALRLPWKVKMMLTAIAVGTDICCRSDGSVNRYLSNLLDFKSPPLKKPKNGLKSF 60
           MS+ALS LRLPWKVKMML AIAVGTDICCRSD SVNR+LSNLLDFKSPPLKKPKNGLKSF
Sbjct: 1   MSDALSTLRLPWKVKMMLIAIAVGTDICCRSDVSVNRFLSNLLDFKSPPLKKPKNGLKSF 60

Query: 61  DVTVDASRNLWFRLYTSTSDSSSGSLPLIVYFHGGGFVYMAPDSKLLDDFCQSLAREISA 120
           DV VDASR+LWFRLY  TS+S S SLPLIVYFHGGGFVYMAPDSKLLDD CQ LAREI A
Sbjct: 61  DVIVDASRDLWFRLYAPTSESMSESLPLIVYFHGGGFVYMAPDSKLLDDLCQRLAREIPA 120

Query: 121 VVISVNYRLAPEYRYPCQYEDGFDVLKFIDGGGSAFEGFPANVDLKRCFLAGDSAGGNIA 180
           VVISVNYRLAPE+R+PCQYED FDVLKFIDG  SA EGFPANV+LKRCFLAGDSAGGNIA
Sbjct: 121 VVISVNYRLAPEHRHPCQYEDAFDVLKFIDGDSSAIEGFPANVNLKRCFLAGDSAGGNIA 180

Query: 181 HHTILKSAEHEFRELEIIGLIAIQPFFGGEERTESEKELIKAPLSTMDRTDWYWKAFLPE 240
           HHTILKS +HE+RELEIIGLI+IQPFFGGE+R ESEK LIKAPLSTMDRTDWYWKAFLPE
Sbjct: 181 HHTILKSGDHEYRELEIIGLISIQPFFGGEKRMESEKRLIKAPLSTMDRTDWYWKAFLPE 240

Query: 241 GSDRDHPAVNIFGPNSVDISKVRFPAIKVFVGGLDPLIDWQKRYYEGLKKSGKEAYLTEY 300
           GSDRDHP+VNIFGPNSVDISKVRFPAIKVFVGGLDPL+DWQK YYEGLKKSGKEAYL+EY
Sbjct: 241 GSDRDHPSVNIFGPNSVDISKVRFPAIKVFVGGLDPLMDWQKEYYEGLKKSGKEAYLSEY 300

Query: 301 PNAFHSFYGFPELPEWPLFIKDAADFVRTQCSKKSS 337
           PNAFHSFYGFPELPEW LFIKD  DF+  QC KKSS
Sbjct: 301 PNAFHSFYGFPELPEWSLFIKDVRDFITEQCLKKSS 336

BLAST of Lag0004094 vs. NCBI nr
Match: KAG6599120.1 (putative carboxylesterase 18, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 618.6 bits (1594), Expect = 3.1e-173
Identity = 294/336 (87.50%), Postives = 312/336 (92.86%), Query Frame = 0

Query: 1   MSEALSALRLPWKVKMMLTAIAVGTDICCRSDGSVNRYLSNLLDFKSPPLKKPKNGLKSF 60
           MS+ALSALRLPWKVKMMLTAIAVGTDIC R D SVNR+L+NL+DFKSPPLKKPKNG+KSF
Sbjct: 1   MSDALSALRLPWKVKMMLTAIAVGTDICGRPDVSVNRFLANLMDFKSPPLKKPKNGVKSF 60

Query: 61  DVTVDASRNLWFRLYTSTSDSSSGSLPLIVYFHGGGFVYMAPDSKLLDDFCQSLAREISA 120
           DVTVDASR+LWFR+Y  TS+S+S SLPLIVYFHGGGFVYMAPDSKL DDFCQSLAREI A
Sbjct: 61  DVTVDASRSLWFRVYFPTSESTSESLPLIVYFHGGGFVYMAPDSKLYDDFCQSLAREIHA 120

Query: 121 VVISVNYRLAPEYRYPCQYEDGFDVLKFIDGGGSAFEGFPANVDLKRCFLAGDSAGGNIA 180
           VVISVNYRLAPE ++PCQYED FDVLKFIDG GS+FEG PANVDLKRCF+AGDSAGGNIA
Sbjct: 121 VVISVNYRLAPENKWPCQYEDSFDVLKFIDGDGSSFEGLPANVDLKRCFVAGDSAGGNIA 180

Query: 181 HHTILKSAEHEFRELEIIGLIAIQPFFGGEERTESEKELIKAPLSTMDRTDWYWKAFLPE 240
           HH ILKS +HEFRELEIIGLIAIQPFFGGEERTES+  LIKAPLST+DRTDWYWKAFLPE
Sbjct: 181 HHMILKSGDHEFRELEIIGLIAIQPFFGGEERTESDIRLIKAPLSTIDRTDWYWKAFLPE 240

Query: 241 GSDRDHPAVNIFGPNSVDISKVRFPAIKVFVGGLDPLIDWQKRYYEGLKKSGKEAYLTEY 300
           G DRDHP+VNIFGP SVDISKVRFPAIKV VGGLDPLIDWQKRYYEGLKKSGKEAYLTEY
Sbjct: 241 GFDRDHPSVNIFGPKSVDISKVRFPAIKVLVGGLDPLIDWQKRYYEGLKKSGKEAYLTEY 300

Query: 301 PNAFHSFYGFPELPEWPLFIKDAADFVRTQCSKKSS 337
           PNAFHSFYGFPELPEW  FIKD ADF+  QCSKKSS
Sbjct: 301 PNAFHSFYGFPELPEWSEFIKDVADFIAAQCSKKSS 336

BLAST of Lag0004094 vs. NCBI nr
Match: KAG7030059.1 (putative carboxylesterase 18, partial [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 617.5 bits (1591), Expect = 7.0e-173
Identity = 293/336 (87.20%), Postives = 312/336 (92.86%), Query Frame = 0

Query: 1   MSEALSALRLPWKVKMMLTAIAVGTDICCRSDGSVNRYLSNLLDFKSPPLKKPKNGLKSF 60
           MS+ALSALRLPWKVKMMLTAIAVGTDIC R D SVNR+L+NL+DFKSPPLKKPKNG+KSF
Sbjct: 1   MSDALSALRLPWKVKMMLTAIAVGTDICGRPDVSVNRFLANLMDFKSPPLKKPKNGVKSF 60

Query: 61  DVTVDASRNLWFRLYTSTSDSSSGSLPLIVYFHGGGFVYMAPDSKLLDDFCQSLAREISA 120
           DVTVDASR+LWFR+Y  TS+S+S SLPLIVYFHGGGFVYMAPDSKL DDFCQSLAREI A
Sbjct: 61  DVTVDASRSLWFRVYFPTSESTSESLPLIVYFHGGGFVYMAPDSKLYDDFCQSLAREIHA 120

Query: 121 VVISVNYRLAPEYRYPCQYEDGFDVLKFIDGGGSAFEGFPANVDLKRCFLAGDSAGGNIA 180
           VVISVNYRLAPE ++PCQYED FDVLKFIDG GS+FEG PANVDLKRCF+AGDSAGGNIA
Sbjct: 121 VVISVNYRLAPENKWPCQYEDSFDVLKFIDGDGSSFEGLPANVDLKRCFVAGDSAGGNIA 180

Query: 181 HHTILKSAEHEFRELEIIGLIAIQPFFGGEERTESEKELIKAPLSTMDRTDWYWKAFLPE 240
           HH ILKS +HEFRELEIIGLIAIQPFFGGEERTES+  LIKAPLST+DRTDWYWKAFLPE
Sbjct: 181 HHMILKSGDHEFRELEIIGLIAIQPFFGGEERTESDIRLIKAPLSTIDRTDWYWKAFLPE 240

Query: 241 GSDRDHPAVNIFGPNSVDISKVRFPAIKVFVGGLDPLIDWQKRYYEGLKKSGKEAYLTEY 300
           G DRDHP+VNIFGP SVDISKVRFPAIKV VGGLDPLIDWQKRYYEGLKKSGKEAYLTEY
Sbjct: 241 GFDRDHPSVNIFGPKSVDISKVRFPAIKVLVGGLDPLIDWQKRYYEGLKKSGKEAYLTEY 300

Query: 301 PNAFHSFYGFPELPEWPLFIKDAADFVRTQCSKKSS 337
           PNAFHSFYGFPELPEW  FIKD ADF+  QCSKKS+
Sbjct: 301 PNAFHSFYGFPELPEWSEFIKDVADFIAAQCSKKST 336

BLAST of Lag0004094 vs. ExPASy Swiss-Prot
Match: Q9LT10 (Probable carboxylesterase 18 OS=Arabidopsis thaliana OX=3702 GN=CXE18 PE=1 SV=1)

HSP 1 Score: 353.2 bits (905), Expect = 3.2e-96
Identity = 176/338 (52.07%), Postives = 226/338 (66.86%), Query Frame = 0

Query: 2   SEALSALRLPWKVKMMLTAIAVGTDICCRSDGSVNRYLSNLLDFKSPPLKKPKNGLKSFD 61
           S+    L LP K ++ LT I+  TD   R DG++NR    L DF++PP  KP N + + D
Sbjct: 5   SQPNQKLTLPLKTRIALTVISTMTDNAQRPDGTINRRFLRLFDFRAPPNPKPVNIVSTSD 64

Query: 62  VTVDASRNLWFRLYTSTSDSSSGSLPLIVYFHGGGFVYMAPDSKLLDDFCQSLAREISAV 121
             VD SR+LWFRLY  T   S   +P++V+FHGGGF +++P++   D+ C+  AR++ A 
Sbjct: 65  FVVDQSRDLWFRLY--TPHVSGDKIPVVVFFHGGGFAFLSPNAYPYDNVCRRFARKLPAY 124

Query: 122 VISVNYRLAPEYRYPCQYEDGFDVLKFIDGGGSAFEGFPANVDLKRCFLAGDSAGGNIAH 181
           VISVNYRLAPE+RYP QY+DGFD LK+I+    +    PAN DL RCF AGDSAGGNIAH
Sbjct: 125 VISVNYRLAPEHRYPAQYDDGFDALKYIEENHGSI--LPANADLSRCFFAGDSAGGNIAH 184

Query: 182 HT---ILKSAEHEFRELEIIGLIAIQPFFGGEERTESEKELIKAPLSTMDRTDWYWKAFL 241
           +    I +     F  +++IGLI+IQPFFGGEERTE+EK+L+ APL + DRTDW WKA  
Sbjct: 185 NVAIRICREPRSSFTAVKLIGLISIQPFFGGEERTEAEKQLVGAPLVSPDRTDWCWKAM- 244

Query: 242 PEGSDRDHPAVNIFGPNSVDISKVRFPAIKVFVGGLDPLIDWQKRYYEGLKKSGKEAYLT 301
             G +RDH AVN+ GPN+VDIS + +P   V V G DPL DWQ+ YYE LK  GK+A L 
Sbjct: 245 --GLNRDHEAVNVGGPNAVDISGLDYPETMVVVAGFDPLKDWQRSYYEWLKLCGKKATLI 304

Query: 302 EYPNAFHSFYGFPELPEWPLFIKDAADFVRTQCSKKSS 337
           EYPN FH+FY FPELPE    I    DFV  + +  S+
Sbjct: 305 EYPNMFHAFYIFPELPEAGQLIMRIKDFVDERVASLSA 335

BLAST of Lag0004094 vs. ExPASy Swiss-Prot
Match: Q940G6 (Gibberellin receptor GID1C OS=Arabidopsis thaliana OX=3702 GN=GID1C PE=1 SV=1)

HSP 1 Score: 254.2 bits (648), Expect = 2.0e-66
Identity = 133/334 (39.82%), Postives = 187/334 (55.99%), Query Frame = 0

Query: 10  LPWKVKMMLTAIAVGTDICCRSDGSVNRYLSNLLDFKSPPLKKPKNGLKSFDVTVDASRN 69
           +P    ++++   +  ++  R DG+ NR+L+  LD K P    P NG+ SFDV +D   N
Sbjct: 16  VPLNTWVLISNFKLAYNLLRRPDGTFNRHLAEFLDRKVPANANPVNGVFSFDVIIDRQTN 75

Query: 70  LWFRLYTSTSDSSSGSL------------PLIVYFHGGGFVYMAPDSKLLDDFCQSLARE 129
           L  R+Y      +S S+            P+IV+FHGG F + + +S + D  C+ L   
Sbjct: 76  LLSRVYRPADAGTSPSITDLQNPVDGEIVPVIVFFHGGSFAHSSANSAIYDTLCRRLVGL 135

Query: 130 ISAVVISVNYRLAPEYRYPCQYEDGFDVLKFIDGGGSAFEGFPANVDLKRCFLAGDSAGG 189
             AVV+SVNYR APE RYPC Y+DG+ VLK+++          + V   R FLAGDS+GG
Sbjct: 136 CGAVVVSVNYRRAPENRYPCAYDDGWAVLKWVNSSSWLRSKKDSKV---RIFLAGDSSGG 195

Query: 190 NIAHHTILKSAEHEFRELEIIGLIAIQPFFGGEERTESEKELIKAPLSTMDRTDWYWKAF 249
           NI H+  +++ E     ++++G I + P FGG ERTESEK L      T+   DWYW+AF
Sbjct: 196 NIVHNVAVRAVE---SRIDVLGNILLNPMFGGTERTESEKRLDGKYFVTVRDRDWYWRAF 255

Query: 250 LPEGSDRDHPAVNIFGPNSVDISKVRFPAIKVFVGGLDPLIDWQKRYYEGLKKSGKEAYL 309
           LPEG DR+HPA + FGP S  +  + FP   V V GLD + DWQ +Y EGLKK+G+E  L
Sbjct: 256 LPEGEDREHPACSPFGPRSKSLEGLSFPKSLVVVAGLDLIQDWQLKYAEGLKKAGQEVKL 315

Query: 310 TEYPNAFHSFYGFPELPEWPLFIKDAADFVRTQC 332
                A   FY  P    +   + + A FV  +C
Sbjct: 316 LYLEQATIGFYLLPNNNHFHTVMDEIAAFVNAEC 343

BLAST of Lag0004094 vs. ExPASy Swiss-Prot
Match: Q9MAA7 (Gibberellin receptor GID1A OS=Arabidopsis thaliana OX=3702 GN=GID1A PE=1 SV=1)

HSP 1 Score: 233.4 bits (594), Expect = 3.7e-60
Identity = 127/336 (37.80%), Postives = 177/336 (52.68%), Query Frame = 0

Query: 10  LPWKVKMMLTAIAVGTDICCRSDGSVNRYLSNLLDFKSPPLKKPKNGLKSFDVTVDASRN 69
           +P    ++++   V  +I  R DG+ NR+L+  LD K      P +G+ SFDV +D   N
Sbjct: 16  VPLNTWVLISNFKVAYNILRRPDGTFNRHLAEYLDRKVTANANPVDGVFSFDVLIDRRIN 75

Query: 70  LWFRLYTST--------------SDSSSGSLPLIVYFHGGGFVYMAPDSKLLDDFCQSLA 129
           L  R+Y                        +P+I++FHGG F + + +S + D  C+ L 
Sbjct: 76  LLSRVYRPAYADQEQPPSILDLEKPVDGDIVPVILFFHGGSFAHSSANSAIYDTLCRRLV 135

Query: 130 REISAVVISVNYRLAPEYRYPCQYEDGFDVLKFIDGGGSAFEGFPANVDLKRCFLAGDSA 189
                VV+SVNYR APE  YPC Y+DG+  L +++          + V +   FLAGDS+
Sbjct: 136 GLCKCVVVSVNYRRAPENPYPCAYDDGWIALNWVNSRSWLKSKKDSKVHI---FLAGDSS 195

Query: 190 GGNIAHHTILKSAEHEFRELEIIGLIAIQPFFGGEERTESEKELIKAPLSTMDRTDWYWK 249
           GGNIAH+  L++ E     ++++G I + P FGG ERTESEK L      T+   DWYWK
Sbjct: 196 GGNIAHNVALRAGE---SGIDVLGNILLNPMFGGNERTESEKSLDGKYFVTVRDRDWYWK 255

Query: 250 AFLPEGSDRDHPAVNIFGPNSVDISKVRFPAIKVFVGGLDPLIDWQKRYYEGLKKSGKEA 309
           AFLPEG DR+HPA N F P    +  V FP   V V GLD + DWQ  Y EGLKK+G+E 
Sbjct: 256 AFLPEGEDREHPACNPFSPRGKSLEGVSFPKSLVVVAGLDLIRDWQLAYAEGLKKAGQEV 315

Query: 310 YLTEYPNAFHSFYGFPELPEWPLFIKDAADFVRTQC 332
            L     A   FY  P    +   + + + FV  +C
Sbjct: 316 KLMHLEKATVGFYLLPNNNHFHNVMDEISAFVNAEC 345

BLAST of Lag0004094 vs. ExPASy Swiss-Prot
Match: Q9LYC1 (Gibberellin receptor GID1B OS=Arabidopsis thaliana OX=3702 GN=GID1B PE=1 SV=1)

HSP 1 Score: 231.9 bits (590), Expect = 1.1e-59
Identity = 124/335 (37.01%), Postives = 185/335 (55.22%), Query Frame = 0

Query: 10  LPWKVKMMLTAIAVGTDICCRSDGSVNRYLSNLLDFKSPPLKKPKNGLKSFDVTVDASRN 69
           +P    ++++   +   +  R DGS NR L+  LD K P    P +G+ SFD  VD++ N
Sbjct: 16  VPLNTWVLISNFKLAYKVLRRPDGSFNRDLAEFLDRKVPANSFPLDGVFSFD-HVDSTTN 75

Query: 70  LWFRLYTSTS---------------DSSSGSLPLIVYFHGGGFVYMAPDSKLLDDFCQSL 129
           L  R+Y   S                S++  +P++++FHGG F + + +S + D FC+ L
Sbjct: 76  LLTRIYQPASLLHQTRHGTLELTKPLSTTEIVPVLIFFHGGSFTHSSANSAIYDTFCRRL 135

Query: 130 AREISAVVISVNYRLAPEYRYPCQYEDGFDVLKFIDGGGSAFEGFPANVDLKRCFLAGDS 189
                 VV+SV+YR +PE+RYPC Y+DG++ L ++        G  +NV +   +LAGDS
Sbjct: 136 VTICGVVVVSVDYRRSPEHRYPCAYDDGWNALNWVKSRVWLQSGKDSNVYV---YLAGDS 195

Query: 190 AGGNIAHHTILKSAEHEFRELEIIGLIAIQPFFGGEERTESEKELIKAPLSTMDRTDWYW 249
           +GGNIAH+  +++       ++++G I + P FGG+ERT+SEK L      T+   DWYW
Sbjct: 196 SGGNIAHNVAVRATN---EGVKVLGNILLHPMFGGQERTQSEKTLDGKYFVTIQDRDWYW 255

Query: 250 KAFLPEGSDRDHPAVNIFGPNSVDISKVRFPAIKVFVGGLDPLIDWQKRYYEGLKKSGKE 309
           +A+LPEG DRDHPA N FGP    +  V FP   V V GLD + DWQ  Y +GLKK+G E
Sbjct: 256 RAYLPEGEDRDHPACNPFGPRGQSLKGVNFPKSLVVVAGLDLVQDWQLAYVDGLKKTGLE 315

Query: 310 AYLTEYPNAFHSFYGFPELPEWPLFIKDAADFVRT 330
             L     A   FY  P    +   +++   FV +
Sbjct: 316 VNLLYLKQATIGFYFLPNNDHFHCLMEELNKFVHS 343

BLAST of Lag0004094 vs. ExPASy Swiss-Prot
Match: Q6L545 (Gibberellin receptor GID1 OS=Oryza sativa subsp. japonica OX=39947 GN=GID1 PE=1 SV=1)

HSP 1 Score: 219.9 bits (559), Expect = 4.3e-56
Identity = 118/339 (34.81%), Postives = 178/339 (52.51%), Query Frame = 0

Query: 10  LPWKVKMMLTAIAVGTDICCRSDGSVNRYLSNLLDFKSPPLKKPKNGLKSFDVTVDASRN 69
           +P    ++++   +  +I  R+DG+  R L   LD + P   +P  G+ SFD  +D S  
Sbjct: 16  VPLHTWVLISNFKLSYNILRRADGTFERDLGEYLDRRVPANARPLEGVSSFDHIIDQSVG 75

Query: 70  LWFRLYTSTS--DSSSGS-------------------LPLIVYFHGGGFVYMAPDSKLLD 129
           L  R+Y + +  D+  G+                    P+I++FHGG FV+ +  S + D
Sbjct: 76  LEVRIYRAAAEGDAEEGAAAVTRPILEFLTDAPAAEPFPVIIFFHGGSFVHSSASSTIYD 135

Query: 130 DFCQSLAREISAVVISVNYRLAPEYRYPCQYEDGFDVLKFIDGGGSAFEGFPANVDLKRC 189
             C+   +    VV+SVNYR APE+RYPC Y+DG+  LK++        G  A     R 
Sbjct: 136 SLCRRFVKLSKGVVVSVNYRRAPEHRYPCAYDDGWTALKWVMSQPFMRSGGDAQA---RV 195

Query: 190 FLAGDSAGGNIAHHTILKSAEHEFRELEIIGLIAIQPFFGGEERTESEKELIKAPLSTMD 249
           FL+GDS+GGNIAHH  +++A+     +++ G I +   FGG ERTESE+ L      T+ 
Sbjct: 196 FLSGDSSGGNIAHHVAVRAAD---EGVKVCGNILLNAMFGGTERTESERRLDGKYFVTLQ 255

Query: 250 RTDWYWKAFLPEGSDRDHPAVNIFGPNSVDISKVRFPAIKVFVGGLDPLIDWQKRYYEGL 309
             DWYWKA+LPE +DRDHPA N FGPN   +  + F    + V GLD   D Q  Y + L
Sbjct: 256 DRDWYWKAYLPEDADRDHPACNPFGPNGRRLGGLPFAKSLIIVSGLDLTCDRQLAYADAL 315

Query: 310 KKSGKEAYLTEYPNAFHSFYGFPELPEWPLFIKDAADFV 328
           ++ G    + +  NA   FY  P    +   +++ +DF+
Sbjct: 316 REDGHHVKVVQCENATVGFYLLPNTVHYHEVMEEISDFL 348

BLAST of Lag0004094 vs. ExPASy TrEMBL
Match: A0A6J1KE39 (probable carboxylesterase 18 OS=Cucurbita maxima OX=3661 GN=LOC111494035 PE=4 SV=1)

HSP 1 Score: 625.9 bits (1613), Expect = 9.5e-176
Identity = 297/336 (88.39%), Postives = 310/336 (92.26%), Query Frame = 0

Query: 1   MSEALSALRLPWKVKMMLTAIAVGTDICCRSDGSVNRYLSNLLDFKSPPLKKPKNGLKSF 60
           MS+ALSALRLPWKVKMMLTAIAVGTDICCR D SVNR+L+NL+DFKSPPLKKPKNG+KSF
Sbjct: 1   MSDALSALRLPWKVKMMLTAIAVGTDICCRRDVSVNRFLANLMDFKSPPLKKPKNGVKSF 60

Query: 61  DVTVDASRNLWFRLYTSTSDSSSGSLPLIVYFHGGGFVYMAPDSKLLDDFCQSLAREISA 120
           DVTVD SRNLWFRLY  TS+S S SLPLI YFHGGGFVYMAPDSKLLDDFCQSLAREI A
Sbjct: 61  DVTVDVSRNLWFRLYAPTSESMSESLPLIFYFHGGGFVYMAPDSKLLDDFCQSLAREIHA 120

Query: 121 VVISVNYRLAPEYRYPCQYEDGFDVLKFIDGGGSAFEGFPANVDLKRCFLAGDSAGGNIA 180
           VVISVNYRLAPE+  PCQYED FDVLKFIDG GS+FEG PANVDLKRCF+AGDSAGGNIA
Sbjct: 121 VVISVNYRLAPEHHCPCQYEDSFDVLKFIDGDGSSFEGLPANVDLKRCFVAGDSAGGNIA 180

Query: 181 HHTILKSAEHEFRELEIIGLIAIQPFFGGEERTESEKELIKAPLSTMDRTDWYWKAFLPE 240
           HH ILKS EH FRELEIIGLIAIQPFFGGEERTESE  L+KAPLST+DRTDWYWKAFLPE
Sbjct: 181 HHMILKSGEHGFRELEIIGLIAIQPFFGGEERTESEIRLLKAPLSTIDRTDWYWKAFLPE 240

Query: 241 GSDRDHPAVNIFGPNSVDISKVRFPAIKVFVGGLDPLIDWQKRYYEGLKKSGKEAYLTEY 300
           G DRDHP+VNIFGP SVDISKVRFPAIKV VGGLDPLIDWQKRYYEGLKKSGKEAYLTEY
Sbjct: 241 GFDRDHPSVNIFGPKSVDISKVRFPAIKVLVGGLDPLIDWQKRYYEGLKKSGKEAYLTEY 300

Query: 301 PNAFHSFYGFPELPEWPLFIKDAADFVRTQCSKKSS 337
           PNAFHSFYGFPELPEWP FIKD ADF+  QCSKKSS
Sbjct: 301 PNAFHSFYGFPELPEWPEFIKDVADFIAAQCSKKSS 336

BLAST of Lag0004094 vs. ExPASy TrEMBL
Match: A0A6J1G4S5 (probable carboxylesterase 18 isoform X1 OS=Cucurbita moschata OX=3662 GN=LOC111450746 PE=4 SV=1)

HSP 1 Score: 611.3 bits (1575), Expect = 2.4e-171
Identity = 291/336 (86.61%), Postives = 309/336 (91.96%), Query Frame = 0

Query: 1   MSEALSALRLPWKVKMMLTAIAVGTDICCRSDGSVNRYLSNLLDFKSPPLKKPKNGLKSF 60
           MS+ALSALRLPWKVKMMLTAIAVGTDIC R D SVNR+L+NL+DFKSPPLKKPKNG+KSF
Sbjct: 1   MSDALSALRLPWKVKMMLTAIAVGTDICGRPDVSVNRFLANLMDFKSPPLKKPKNGVKSF 60

Query: 61  DVTVDASRNLWFRLYTSTSDSSSGSLPLIVYFHGGGFVYMAPDSKLLDDFCQSLAREISA 120
           DVTVDASRNLWFRLY  +S+S+S SLPLI YFHGGGFVYMAPDSKLLDDFCQSLAREI A
Sbjct: 61  DVTVDASRNLWFRLYFPSSESTSESLPLIFYFHGGGFVYMAPDSKLLDDFCQSLAREIHA 120

Query: 121 VVISVNYRLAPEYRYPCQYEDGFDVLKFIDGGGSAFEGFPANVDLKRCFLAGDSAGGNIA 180
           VVISVNYRLAPE + PCQYED FDVLKFIDG GS+FEG PANVDLKRCF+AGDSAGGNIA
Sbjct: 121 VVISVNYRLAPENKCPCQYEDSFDVLKFIDGEGSSFEGLPANVDLKRCFVAGDSAGGNIA 180

Query: 181 HHTILKSAEHEFRELEIIGLIAIQPFFGGEERTESEKELIKAPLSTMDRTDWYWKAFLPE 240
           HH ILKS +HEFR+LEIIGLIAIQPFFGGEERTES+  LIKAPLST+DRTDWYWKAFLPE
Sbjct: 181 HHMILKSGDHEFRKLEIIGLIAIQPFFGGEERTESDIRLIKAPLSTIDRTDWYWKAFLPE 240

Query: 241 GSDRDHPAVNIFGPNSVDISKVRFPAIKVFVGGLDPLIDWQKRYYEGLKKSGKEAYLTEY 300
           G DRDHP+VNIFGP SVDIS VRFPAIKV VGGLDPLIDWQKRYYEGLKKSGKEAYLTEY
Sbjct: 241 GFDRDHPSVNIFGPKSVDISNVRFPAIKVVVGGLDPLIDWQKRYYEGLKKSGKEAYLTEY 300

Query: 301 PNAFHSFYGFPELPEWPLFIKDAADFVRTQCSKKSS 337
           PNAFHSFYGFPELPEW  FIKD ADF+  QC K+SS
Sbjct: 301 PNAFHSFYGFPELPEWSEFIKDVADFIAAQCKKQSS 336

BLAST of Lag0004094 vs. ExPASy TrEMBL
Match: A0A5A7T6B5 (Putative carboxylesterase 18 OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold119G00400 PE=4 SV=1)

HSP 1 Score: 591.7 bits (1524), Expect = 2.0e-165
Identity = 277/336 (82.44%), Postives = 303/336 (90.18%), Query Frame = 0

Query: 1   MSEALSALRLPWKVKMMLTAIAVGTDICCRSDGSVNRYLSNLLDFKSPPLKKPKNGLKSF 60
           MS+ALS+LRLPWK+KM+L+AI++GTDICCRSD SVNR+L+N LDFKSPPLKKPKNG+KSF
Sbjct: 1   MSDALSSLRLPWKIKMILSAISLGTDICCRSDVSVNRFLANFLDFKSPPLKKPKNGVKSF 60

Query: 61  DVTVDASRNLWFRLYTSTSDSSSGSLPLIVYFHGGGFVYMAPDSKLLDDFCQSLAREISA 120
           D TVD+SRNLWFRLYT T++S+S SLPLIVY HGGGFVYMAPDSKLLD+ CQ LAREI A
Sbjct: 61  DTTVDSSRNLWFRLYTPTTESTSESLPLIVYCHGGGFVYMAPDSKLLDELCQRLAREIPA 120

Query: 121 VVISVNYRLAPEYRYPCQYEDGFDVLKFIDGGGSAFEGFPANVDLKRCFLAGDSAGGNIA 180
           VVIS+NYRLAPE+RYPCQYED FD+LKFID   SA EGFP NVDLKRCFLAGDSAGGNIA
Sbjct: 121 VVISLNYRLAPEHRYPCQYEDAFDILKFIDNNASAIEGFPPNVDLKRCFLAGDSAGGNIA 180

Query: 181 HHTILKSAEHEFRELEIIGLIAIQPFFGGEERTESEKELIKAPLSTMDRTDWYWKAFLPE 240
           HH ILKS +HE RELEIIGLI+IQPFFGGEER ESE +LIKAPLST+DRTDWYWKAFLPE
Sbjct: 181 HHVILKSGDHEHRELEIIGLISIQPFFGGEERLESEIKLIKAPLSTIDRTDWYWKAFLPE 240

Query: 241 GSDRDHPAVNIFGPNSVDISKVRFPAIKVFVGGLDPLIDWQKRYYEGLKKSGKEAYLTEY 300
           G DRDHP+VN+FGPNS DIS VRFPA KV VGGLDPLIDWQKRYYEGLKKSGKEAYL+EY
Sbjct: 241 GCDRDHPSVNVFGPNSTDISNVRFPATKVLVGGLDPLIDWQKRYYEGLKKSGKEAYLSEY 300

Query: 301 PNAFHSFYGFPELPEWPLFIKDAADFVRTQCSKKSS 337
           PN FHSFYGFPEL E  LFIKD  DFV+ QC KKSS
Sbjct: 301 PNGFHSFYGFPELEESNLFIKDIRDFVKEQCLKKSS 336

BLAST of Lag0004094 vs. ExPASy TrEMBL
Match: A0A1S3CP40 (probable carboxylesterase 18 OS=Cucumis melo OX=3656 GN=LOC103502644 PE=4 SV=1)

HSP 1 Score: 590.9 bits (1522), Expect = 3.4e-165
Identity = 276/336 (82.14%), Postives = 303/336 (90.18%), Query Frame = 0

Query: 1   MSEALSALRLPWKVKMMLTAIAVGTDICCRSDGSVNRYLSNLLDFKSPPLKKPKNGLKSF 60
           MS+ALS+LRLPWK+KM+L+AI++GTDICCRSD SVNR+L+N LDFKSPPLKKPKNG+KSF
Sbjct: 1   MSDALSSLRLPWKIKMILSAISLGTDICCRSDVSVNRFLANFLDFKSPPLKKPKNGVKSF 60

Query: 61  DVTVDASRNLWFRLYTSTSDSSSGSLPLIVYFHGGGFVYMAPDSKLLDDFCQSLAREISA 120
           D TVD+SRNLWFRLYT T++S+S SLPLIVY HGGGFVYMAPDSKLLD+ CQ LAREI A
Sbjct: 61  DTTVDSSRNLWFRLYTPTTESTSQSLPLIVYCHGGGFVYMAPDSKLLDELCQRLAREIPA 120

Query: 121 VVISVNYRLAPEYRYPCQYEDGFDVLKFIDGGGSAFEGFPANVDLKRCFLAGDSAGGNIA 180
           VVIS+NYRLAPE+RYPCQYED FD+LKFID   SA EGFP NVDLKRCFLAGDSAGGNIA
Sbjct: 121 VVISLNYRLAPEHRYPCQYEDAFDILKFIDNNASAIEGFPPNVDLKRCFLAGDSAGGNIA 180

Query: 181 HHTILKSAEHEFRELEIIGLIAIQPFFGGEERTESEKELIKAPLSTMDRTDWYWKAFLPE 240
           HH ILKS +HE RELEIIGLI+IQPFFGGEER ESE +LIKAPLST+DRTDWYWKAFLPE
Sbjct: 181 HHVILKSGDHEHRELEIIGLISIQPFFGGEERLESEIKLIKAPLSTIDRTDWYWKAFLPE 240

Query: 241 GSDRDHPAVNIFGPNSVDISKVRFPAIKVFVGGLDPLIDWQKRYYEGLKKSGKEAYLTEY 300
           G DRDHP+VN+FGPNS DIS VRFPA KV VGGLDPLIDWQKRYYEGLKKSGKEAYL+EY
Sbjct: 241 GCDRDHPSVNVFGPNSTDISNVRFPATKVLVGGLDPLIDWQKRYYEGLKKSGKEAYLSEY 300

Query: 301 PNAFHSFYGFPELPEWPLFIKDAADFVRTQCSKKSS 337
           PN FHSFYGFPEL E  +FIKD  DFV+ QC KKSS
Sbjct: 301 PNGFHSFYGFPELEESNMFIKDIRDFVKEQCLKKSS 336

BLAST of Lag0004094 vs. ExPASy TrEMBL
Match: A0A6J1KHY2 (probable carboxylesterase 18 OS=Cucurbita maxima OX=3661 GN=LOC111493993 PE=4 SV=1)

HSP 1 Score: 590.1 bits (1520), Expect = 5.8e-165
Identity = 278/336 (82.74%), Postives = 304/336 (90.48%), Query Frame = 0

Query: 1   MSEALSALRLPWKVKMMLTAIAVGTDICCRSDGSVNRYLSNLLDFKSPPLKKPKNGLKSF 60
           MS+ALSALRLPWKVK++LT+I +GTDIC R DGSVNR++ NL+DFKS PLKKPKNG+KSF
Sbjct: 1   MSDALSALRLPWKVKIILTSITIGTDICSRPDGSVNRFIFNLMDFKSSPLKKPKNGVKSF 60

Query: 61  DVTVDASRNLWFRLYTSTSDSSSGSLPLIVYFHGGGFVYMAPDSKLLDDFCQSLAREISA 120
           DVTVDASRNLWFRLY  TS+S+S SLPLIVYFHGGGFVYMAPDSKL DDFCQSL+REI A
Sbjct: 61  DVTVDASRNLWFRLYFPTSESTSESLPLIVYFHGGGFVYMAPDSKLYDDFCQSLSREIHA 120

Query: 121 VVISVNYRLAPEYRYPCQYEDGFDVLKFIDGGGSAFEGFPANVDLKRCFLAGDSAGGNIA 180
           VVISVNYRLAPE ++PCQYED FDVLKFIDG GS+FEG  ANVDLKRCF+AGDSAGGNIA
Sbjct: 121 VVISVNYRLAPENKWPCQYEDSFDVLKFIDGDGSSFEGLAANVDLKRCFVAGDSAGGNIA 180

Query: 181 HHTILKSAEHEFRELEIIGLIAIQPFFGGEERTESEKELIKAPLSTMDRTDWYWKAFLPE 240
           HH ILKS +HEFR+LEIIGLIAIQPFFGGEERTESE  L+KAPL T+DR DW+WKAFLPE
Sbjct: 181 HHMILKSGDHEFRKLEIIGLIAIQPFFGGEERTESEIRLLKAPLLTIDRADWFWKAFLPE 240

Query: 241 GSDRDHPAVNIFGPNSVDISKVRFPAIKVFVGGLDPLIDWQKRYYEGLKKSGKEAYLTEY 300
           G DRDH +VNIFGP S DISKVRFPAIKVFVGGLDPLID QKRYYEGLKKSGKEAYLTEY
Sbjct: 241 GFDRDHKSVNIFGPKSEDISKVRFPAIKVFVGGLDPLIDRQKRYYEGLKKSGKEAYLTEY 300

Query: 301 PNAFHSFYGFPELPEWPLFIKDAADFVRTQCSKKSS 337
           PNAFH+FYGFPELPEW  FIKD ADF+  +C K+SS
Sbjct: 301 PNAFHTFYGFPELPEWSKFIKDVADFIAAECKKQSS 336

BLAST of Lag0004094 vs. TAIR 10
Match: AT5G23530.1 (carboxyesterase 18 )

HSP 1 Score: 353.2 bits (905), Expect = 2.3e-97
Identity = 176/338 (52.07%), Postives = 226/338 (66.86%), Query Frame = 0

Query: 2   SEALSALRLPWKVKMMLTAIAVGTDICCRSDGSVNRYLSNLLDFKSPPLKKPKNGLKSFD 61
           S+    L LP K ++ LT I+  TD   R DG++NR    L DF++PP  KP N + + D
Sbjct: 5   SQPNQKLTLPLKTRIALTVISTMTDNAQRPDGTINRRFLRLFDFRAPPNPKPVNIVSTSD 64

Query: 62  VTVDASRNLWFRLYTSTSDSSSGSLPLIVYFHGGGFVYMAPDSKLLDDFCQSLAREISAV 121
             VD SR+LWFRLY  T   S   +P++V+FHGGGF +++P++   D+ C+  AR++ A 
Sbjct: 65  FVVDQSRDLWFRLY--TPHVSGDKIPVVVFFHGGGFAFLSPNAYPYDNVCRRFARKLPAY 124

Query: 122 VISVNYRLAPEYRYPCQYEDGFDVLKFIDGGGSAFEGFPANVDLKRCFLAGDSAGGNIAH 181
           VISVNYRLAPE+RYP QY+DGFD LK+I+    +    PAN DL RCF AGDSAGGNIAH
Sbjct: 125 VISVNYRLAPEHRYPAQYDDGFDALKYIEENHGSI--LPANADLSRCFFAGDSAGGNIAH 184

Query: 182 HT---ILKSAEHEFRELEIIGLIAIQPFFGGEERTESEKELIKAPLSTMDRTDWYWKAFL 241
           +    I +     F  +++IGLI+IQPFFGGEERTE+EK+L+ APL + DRTDW WKA  
Sbjct: 185 NVAIRICREPRSSFTAVKLIGLISIQPFFGGEERTEAEKQLVGAPLVSPDRTDWCWKAM- 244

Query: 242 PEGSDRDHPAVNIFGPNSVDISKVRFPAIKVFVGGLDPLIDWQKRYYEGLKKSGKEAYLT 301
             G +RDH AVN+ GPN+VDIS + +P   V V G DPL DWQ+ YYE LK  GK+A L 
Sbjct: 245 --GLNRDHEAVNVGGPNAVDISGLDYPETMVVVAGFDPLKDWQRSYYEWLKLCGKKATLI 304

Query: 302 EYPNAFHSFYGFPELPEWPLFIKDAADFVRTQCSKKSS 337
           EYPN FH+FY FPELPE    I    DFV  + +  S+
Sbjct: 305 EYPNMFHAFYIFPELPEAGQLIMRIKDFVDERVASLSA 335

BLAST of Lag0004094 vs. TAIR 10
Match: AT5G27320.1 (alpha/beta-Hydrolases superfamily protein )

HSP 1 Score: 254.2 bits (648), Expect = 1.4e-67
Identity = 133/334 (39.82%), Postives = 187/334 (55.99%), Query Frame = 0

Query: 10  LPWKVKMMLTAIAVGTDICCRSDGSVNRYLSNLLDFKSPPLKKPKNGLKSFDVTVDASRN 69
           +P    ++++   +  ++  R DG+ NR+L+  LD K P    P NG+ SFDV +D   N
Sbjct: 16  VPLNTWVLISNFKLAYNLLRRPDGTFNRHLAEFLDRKVPANANPVNGVFSFDVIIDRQTN 75

Query: 70  LWFRLYTSTSDSSSGSL------------PLIVYFHGGGFVYMAPDSKLLDDFCQSLARE 129
           L  R+Y      +S S+            P+IV+FHGG F + + +S + D  C+ L   
Sbjct: 76  LLSRVYRPADAGTSPSITDLQNPVDGEIVPVIVFFHGGSFAHSSANSAIYDTLCRRLVGL 135

Query: 130 ISAVVISVNYRLAPEYRYPCQYEDGFDVLKFIDGGGSAFEGFPANVDLKRCFLAGDSAGG 189
             AVV+SVNYR APE RYPC Y+DG+ VLK+++          + V   R FLAGDS+GG
Sbjct: 136 CGAVVVSVNYRRAPENRYPCAYDDGWAVLKWVNSSSWLRSKKDSKV---RIFLAGDSSGG 195

Query: 190 NIAHHTILKSAEHEFRELEIIGLIAIQPFFGGEERTESEKELIKAPLSTMDRTDWYWKAF 249
           NI H+  +++ E     ++++G I + P FGG ERTESEK L      T+   DWYW+AF
Sbjct: 196 NIVHNVAVRAVE---SRIDVLGNILLNPMFGGTERTESEKRLDGKYFVTVRDRDWYWRAF 255

Query: 250 LPEGSDRDHPAVNIFGPNSVDISKVRFPAIKVFVGGLDPLIDWQKRYYEGLKKSGKEAYL 309
           LPEG DR+HPA + FGP S  +  + FP   V V GLD + DWQ +Y EGLKK+G+E  L
Sbjct: 256 LPEGEDREHPACSPFGPRSKSLEGLSFPKSLVVVAGLDLIQDWQLKYAEGLKKAGQEVKL 315

Query: 310 TEYPNAFHSFYGFPELPEWPLFIKDAADFVRTQC 332
                A   FY  P    +   + + A FV  +C
Sbjct: 316 LYLEQATIGFYLLPNNNHFHTVMDEIAAFVNAEC 343

BLAST of Lag0004094 vs. TAIR 10
Match: AT3G05120.1 (alpha/beta-Hydrolases superfamily protein )

HSP 1 Score: 233.4 bits (594), Expect = 2.6e-61
Identity = 127/336 (37.80%), Postives = 177/336 (52.68%), Query Frame = 0

Query: 10  LPWKVKMMLTAIAVGTDICCRSDGSVNRYLSNLLDFKSPPLKKPKNGLKSFDVTVDASRN 69
           +P    ++++   V  +I  R DG+ NR+L+  LD K      P +G+ SFDV +D   N
Sbjct: 16  VPLNTWVLISNFKVAYNILRRPDGTFNRHLAEYLDRKVTANANPVDGVFSFDVLIDRRIN 75

Query: 70  LWFRLYTST--------------SDSSSGSLPLIVYFHGGGFVYMAPDSKLLDDFCQSLA 129
           L  R+Y                        +P+I++FHGG F + + +S + D  C+ L 
Sbjct: 76  LLSRVYRPAYADQEQPPSILDLEKPVDGDIVPVILFFHGGSFAHSSANSAIYDTLCRRLV 135

Query: 130 REISAVVISVNYRLAPEYRYPCQYEDGFDVLKFIDGGGSAFEGFPANVDLKRCFLAGDSA 189
                VV+SVNYR APE  YPC Y+DG+  L +++          + V +   FLAGDS+
Sbjct: 136 GLCKCVVVSVNYRRAPENPYPCAYDDGWIALNWVNSRSWLKSKKDSKVHI---FLAGDSS 195

Query: 190 GGNIAHHTILKSAEHEFRELEIIGLIAIQPFFGGEERTESEKELIKAPLSTMDRTDWYWK 249
           GGNIAH+  L++ E     ++++G I + P FGG ERTESEK L      T+   DWYWK
Sbjct: 196 GGNIAHNVALRAGE---SGIDVLGNILLNPMFGGNERTESEKSLDGKYFVTVRDRDWYWK 255

Query: 250 AFLPEGSDRDHPAVNIFGPNSVDISKVRFPAIKVFVGGLDPLIDWQKRYYEGLKKSGKEA 309
           AFLPEG DR+HPA N F P    +  V FP   V V GLD + DWQ  Y EGLKK+G+E 
Sbjct: 256 AFLPEGEDREHPACNPFSPRGKSLEGVSFPKSLVVVAGLDLIRDWQLAYAEGLKKAGQEV 315

Query: 310 YLTEYPNAFHSFYGFPELPEWPLFIKDAADFVRTQC 332
            L     A   FY  P    +   + + + FV  +C
Sbjct: 316 KLMHLEKATVGFYLLPNNNHFHNVMDEISAFVNAEC 345

BLAST of Lag0004094 vs. TAIR 10
Match: AT3G63010.1 (alpha/beta-Hydrolases superfamily protein )

HSP 1 Score: 231.9 bits (590), Expect = 7.7e-61
Identity = 124/335 (37.01%), Postives = 185/335 (55.22%), Query Frame = 0

Query: 10  LPWKVKMMLTAIAVGTDICCRSDGSVNRYLSNLLDFKSPPLKKPKNGLKSFDVTVDASRN 69
           +P    ++++   +   +  R DGS NR L+  LD K P    P +G+ SFD  VD++ N
Sbjct: 16  VPLNTWVLISNFKLAYKVLRRPDGSFNRDLAEFLDRKVPANSFPLDGVFSFD-HVDSTTN 75

Query: 70  LWFRLYTSTS---------------DSSSGSLPLIVYFHGGGFVYMAPDSKLLDDFCQSL 129
           L  R+Y   S                S++  +P++++FHGG F + + +S + D FC+ L
Sbjct: 76  LLTRIYQPASLLHQTRHGTLELTKPLSTTEIVPVLIFFHGGSFTHSSANSAIYDTFCRRL 135

Query: 130 AREISAVVISVNYRLAPEYRYPCQYEDGFDVLKFIDGGGSAFEGFPANVDLKRCFLAGDS 189
                 VV+SV+YR +PE+RYPC Y+DG++ L ++        G  +NV +   +LAGDS
Sbjct: 136 VTICGVVVVSVDYRRSPEHRYPCAYDDGWNALNWVKSRVWLQSGKDSNVYV---YLAGDS 195

Query: 190 AGGNIAHHTILKSAEHEFRELEIIGLIAIQPFFGGEERTESEKELIKAPLSTMDRTDWYW 249
           +GGNIAH+  +++       ++++G I + P FGG+ERT+SEK L      T+   DWYW
Sbjct: 196 SGGNIAHNVAVRATN---EGVKVLGNILLHPMFGGQERTQSEKTLDGKYFVTIQDRDWYW 255

Query: 250 KAFLPEGSDRDHPAVNIFGPNSVDISKVRFPAIKVFVGGLDPLIDWQKRYYEGLKKSGKE 309
           +A+LPEG DRDHPA N FGP    +  V FP   V V GLD + DWQ  Y +GLKK+G E
Sbjct: 256 RAYLPEGEDRDHPACNPFGPRGQSLKGVNFPKSLVVVAGLDLVQDWQLAYVDGLKKTGLE 315

Query: 310 AYLTEYPNAFHSFYGFPELPEWPLFIKDAADFVRT 330
             L     A   FY  P    +   +++   FV +
Sbjct: 316 VNLLYLKQATIGFYFLPNNDHFHCLMEELNKFVHS 343

BLAST of Lag0004094 vs. TAIR 10
Match: AT5G62180.1 (carboxyesterase 20 )

HSP 1 Score: 187.2 bits (474), Expect = 2.2e-47
Identity = 110/273 (40.29%), Postives = 153/273 (56.04%), Query Frame = 0

Query: 32  DGSVNRYLSNLLDFKSPPLKKPKNGLKSFDVTVDASRNLWFRLYTSTS-----DSSSGSL 91
           DGS+ R LSN     + P   P N   S D+ V+  ++ W RLY  +S     + SS  L
Sbjct: 21  DGSITRDLSNFPCTAATPDPSPLNPAVSKDLPVNQLKSTWLRLYLPSSAVNEGNVSSQKL 80

Query: 92  PLIVYFHGGGFVYMAPDSKLLDDFCQSLAREISAVVISVNYRLAPEYRYPCQYEDGFDVL 151
           P++VY+HGGGF+  + D +L  DFC  +AR+++A+V+S +YRLAPE+R P  Y+DG + L
Sbjct: 81  PIVVYYHGGGFILCSVDMQLFHDFCSEVARDLNAIVVSPSYRLAPEHRLPAAYDDGVEAL 140

Query: 152 KFIDGGGSAFEGFPANVDLKRCFLAGDSAGGNIAHHTILKSAE--HEFRELEIIGLIAIQ 211
            +I    S  E   ++ D    FL G SAGGN+A++  L+S +   +   L+I GLI   
Sbjct: 141 DWIK--TSDDEWIKSHADFSNVFLMGTSAGGNLAYNVGLRSVDSVSDLSPLQIRGLILHH 200

Query: 212 PFFGGEERTESEKELIKAPLSTMDRTDWYWKAFLPEGSDRDHPAVN-IFGPNSVDISKVR 271
           PFFGGEER+ESE  L+   +     TD  W   LP G DRDH   N   G  S  + K+ 
Sbjct: 201 PFFGGEERSESEIRLMNDQVCPPIVTDVMWDLSLPVGVDRDHEYSNPTVGDGSEKLEKIG 260

Query: 272 FPAIKVFV--GGLDPLIDWQKRYYEGLKKSGKE 295
               KV +  G  DP+ID QK   + +KK G E
Sbjct: 261 RLRWKVMMIGGEDDPMIDLQKDVAKLMKKKGVE 291