Lag0004087 (gene) Sponge gourd (AG‐4) v1

BLAST of Lag0004087 vs. NCBI nr
Match: XP_038885883.1 (neutral ceramidase 2-like [Benincasa hispida])

HSP 1 Score: 1337.4 bits (3460), Expect = 0.0e+00
Identity = 673/825 (81.58%), Postives = 703/825 (85.21%), Query Frame = 0

Query: 10  MGTVIAAASWCCVNGDWLVGVGSFDMTGPAAQVNMMGYANMDQNTAGVHFRLRARTFVVA 69
           M TVIAA SW CVNGDWLVGVGSFDMTGPAA VNM+GYANMDQNTAG+HFRLRARTFVVA
Sbjct: 1   MATVIAAVSW-CVNGDWLVGVGSFDMTGPAAGVNMIGYANMDQNTAGIHFRLRARTFVVA 60

Query: 70  KTVDGPRFAFVNLDAGMASQLVTIKVLERLKSRFGDLYTEENVAISGIHTHAGPGGYLQY 129
           KTVDGPR AF+NLDAGMASQLVTIKVLERLKSRFGDLYTEENVAISGIHTHAGPGGYLQY
Sbjct: 61  KTVDGPRIAFINLDAGMASQLVTIKVLERLKSRFGDLYTEENVAISGIHTHAGPGGYLQY 120

Query: 130 LVYSITSLGFVQQSFDAIVNAIEQSIIQAHQSLKPGSIFINKGDVVNAGINRSPSAYLLN 189
           LVYSITSLGFVQQSFDAIVNAIEQSIIQAH+SLKPG I  NKGDV N GINRSPSAYL+N
Sbjct: 121 LVYSITSLGFVQQSFDAIVNAIEQSIIQAHESLKPGFILFNKGDVENGGINRSPSAYLMN 180

Query: 190 PQEERARYSTNVDTEMTVLKFVDGASGKSVGAFSWFPTHGTSMSRDNKLISGDNKGAAAR 249
           P+EERARY+ NVD EM+VLKFVDG SG S+GAFSWFPTHGTSMSRDNKLISGDNKGAAAR
Sbjct: 181 PEEERARYANNVDKEMSVLKFVDGESGNSIGAFSWFPTHGTSMSRDNKLISGDNKGAAAR 240

Query: 250 FFEDWIASKRTKRSSNNNASDFNEMGREIFLSCQDFNEMGREILLKLLSVGVGEKSASEL 309
           FFEDW+ASKRTK +S+NN S                                        
Sbjct: 241 FFEDWVASKRTKITSSNNTS---------------------------------------- 300

Query: 310 GRNGGIGGSELIGVRSEGERNDGIGGSELVGVRSGRETTASAATALVDIVELMKKAETIK 369
                                                           IVELM+KAETIK
Sbjct: 301 -----------------------------------------------GIVELMRKAETIK 360

Query: 370 ATGGKRCSKASSQASKVRNNDGSLFVAAFCQSNVGDVTPNVLGAFCTDSGNPCDFNRSSC 429
           ATGGKRCSK SSQASKVR NDGSLFVAAFCQSNVGDVTPNVLGAFC DSG PCDFNRSSC
Sbjct: 361 ATGGKRCSKTSSQASKVRKNDGSLFVAAFCQSNVGDVTPNVLGAFCIDSGKPCDFNRSSC 420

Query: 430 HGNDLLCVGRGPGFPDEILSTKIIGERQFLKAADLFTTATEKLTGEIDFRHVYLNFTDIE 489
           HG+DLLCVGRGPGFPDEILSTKIIGERQF KAADLFTTATEKLTGEIDFRHVYLNFTDIE
Sbjct: 421 HGSDLLCVGRGPGFPDEILSTKIIGERQFQKAADLFTTATEKLTGEIDFRHVYLNFTDIE 480

Query: 490 VELDGDNVVRTCPAALGPGFAAGTTDGPGVFGFQQGDTKINELWRKLRDSLRKPSEFQVG 549
           VELDG+NVV+TCPAALGPGFAAGTTDGPGVFGFQQGDT+IN+LW+KLRDSL+KPSEFQVG
Sbjct: 481 VELDGNNVVKTCPAALGPGFAAGTTDGPGVFGFQQGDTEINKLWKKLRDSLKKPSEFQVG 540

Query: 550 CQKPKTVLLDTGEMFEPYAWAPAILPIQILRLGKLIILSVPGEFTTMAGRRLREAVKETL 609
           CQKPKTVLLDTGEMFEPYAWAPAILPIQILRLGKLIILSVPGEFTTMAGR+LREAVKETL
Sbjct: 541 CQKPKTVLLDTGEMFEPYAWAPAILPIQILRLGKLIILSVPGEFTTMAGRQLREAVKETL 600

Query: 610 ISNGNGEFDDDTHVVIAGLTNTYSQYVATFEEYEQQRYEAASTLYGPHTLSAYIQEFKKL 669
           I NGNGEFDDDTHVVIAGLTNTYSQYVATFEEYEQQRYEAASTLYGPHTLSAYIQEFKKL
Sbjct: 601 IRNGNGEFDDDTHVVIAGLTNTYSQYVATFEEYEQQRYEAASTLYGPHTLSAYIQEFKKL 660

Query: 670 ATAIAKGENIVKPIASPPDLSSVQLRFVLEPFGESPPNGINFGDIQQDIKLPKTGWFKRG 729
           A AIAK ENI KPIASPPDLSSVQLRFV++PFGESPPNGINFGDIQQDIKLP  GWFKRG
Sbjct: 661 AIAIAKHENIAKPIASPPDLSSVQLRFVIDPFGESPPNGINFGDIQQDIKLPTNGWFKRG 720

Query: 730 DKQKPTATFWTANPRFDLLTEGTYAVVERLEKQRWKPAYDDDDFSLLFKWTLDNNSFINS 789
            KQKP ATFW+ANPRFDLLTEGTYAVVERLEKQRW P YDDDDFSL FKWTLDNN+FINS
Sbjct: 721 GKQKPAATFWSANPRFDLLTEGTYAVVERLEKQRWTPVYDDDDFSLFFKWTLDNNTFINS 737

Query: 790 LATIEWDIPTDADPGVYRLRHFGSSKTTTNSSNIYFSGASRAFAV 835
           +ATIEWDIP DADPGVYRLRHFGSSK+T NS+NIYF+GAS+AFAV
Sbjct: 781 IATIEWDIPIDADPGVYRLRHFGSSKSTINSTNIYFTGASQAFAV 737

BLAST of Lag0004087 vs. NCBI nr
Match: KAG7030054.1 (Neutral ceramidase 2, partial [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 1294.3 bits (3348), Expect = 0.0e+00
Identity = 658/851 (77.32%), Postives = 701/851 (82.37%), Query Frame = 0

Query: 1   MRRPLLKLIMGTVIAAASWCC-VNGDWLVGVGSFDMTGPAAQVNMMGYANMDQNTAGVHF 60
           MRR LLK++   V+AA S CC  +GDWLVGVGSFDMTGPAA+VNMMGYANMDQ+ AG+HF
Sbjct: 1   MRRQLLKVVATAVLAAVSCCCAADGDWLVGVGSFDMTGPAAEVNMMGYANMDQSAAGIHF 60

Query: 61  RLRARTFVVAKTVDGPRFAFVNLDAGMASQLVTIKVLERLKS---------------RFG 120
           RLRARTF+VAK+VDGPR AFVNLDAGMASQLVTIK+LERLK+               RFG
Sbjct: 61  RLRARTFIVAKSVDGPRIAFVNLDAGMASQLVTIKLLERLKASLTFLSKLKPRHFIQRFG 120

Query: 121 DLYTEENVAISGIHTHAGPGGYLQYLVYSITSLGFVQQSFDAIVNAIEQSIIQAHQSLKP 180
           +LYTEENVAISGIHTHAGPGGYLQYLVYSITSLGFVQQSFDAIVNAIEQSI+QAH SLKP
Sbjct: 121 NLYTEENVAISGIHTHAGPGGYLQYLVYSITSLGFVQQSFDAIVNAIEQSIVQAHGSLKP 180

Query: 181 GSIFINKGDVVNAGINRSPSAYLLNPQEERARYSTNVDTEMTVLKFVDGASGKSVGAFSW 240
           G I INKG+V NAGINRSPSAYL+NP EERARY TNVDTEM+++K V+G SG S+GAFSW
Sbjct: 181 GFILINKGEVENAGINRSPSAYLMNPPEERARYPTNVDTEMSLVKVVEGGSGVSLGAFSW 240

Query: 241 FPTHGTSMSRDNKLISGDNKGAAARFFEDWI-ASKRTKRSSNNNASDFNEMGREIFLSCQ 300
           FPTHGTSMSRDNKLISGDNKGAAARFFEDW+ AS R+ R++++N S              
Sbjct: 241 FPTHGTSMSRDNKLISGDNKGAAARFFEDWVAASNRSTRATSSNKS-------------- 300

Query: 301 DFNEMGREILLKLLSVGVGEKSASELGRNGGIGGSELIGVRSEGERNDGIGGSELVGVRS 360
                                                                       
Sbjct: 301 ------------------------------------------------------------ 360

Query: 361 GRETTASAATALVDIVELMKKAETIKATGGKRCSKASSQASKVRNNDGSLFVAAFCQSNV 420
                        DIVELMKKAETIKATGGKRCSK SSQ+SKVR NDGSLFV AFCQSNV
Sbjct: 361 -------------DIVELMKKAETIKATGGKRCSKTSSQSSKVRKNDGSLFVGAFCQSNV 420

Query: 421 GDVTPNVLGAFCTDSGNPCDFNRSSCHGNDLLCVGRGPGFPDEILSTKIIGERQFLKAAD 480
           GDVTPNVLGAFCTDSG PCDFNRSSCHG+DLLCVGRGPGFPDEILSTKIIGERQFLKA D
Sbjct: 421 GDVTPNVLGAFCTDSGKPCDFNRSSCHGSDLLCVGRGPGFPDEILSTKIIGERQFLKAVD 480

Query: 481 LFTTATEKLTGEIDFRHVYLNFTDIEVELDGDNVVRTCPAALGPGFAAGTTDGPGVFGFQ 540
           LFTTATE+LTGEIDFRHVYLNFTDIEVEL GDNVV+TCPAALGPGFAAGTTDGPGVFGFQ
Sbjct: 481 LFTTATERLTGEIDFRHVYLNFTDIEVELGGDNVVKTCPAALGPGFAAGTTDGPGVFGFQ 540

Query: 541 QGDTKINELWRKLRDSLRKPSEFQVGCQKPKTVLLDTGEMFEPYAWAPAILPIQILRLGK 600
           QGDT+IN+LW+KLRDSLRKPSEFQVGCQKPKTVLLDTGEMFEPYAWAPAILPIQILRLGK
Sbjct: 541 QGDTQINKLWKKLRDSLRKPSEFQVGCQKPKTVLLDTGEMFEPYAWAPAILPIQILRLGK 600

Query: 601 LIILSVPGEFTTMAGRRLREAVKETLISNGNGEFDDDTHVVIAGLTNTYSQYVATFEEYE 660
           LIILSVPGEFTTMAGRRLREAVKETLISNGNGEFDDDTHVVIAGLTN YSQYVAT EEYE
Sbjct: 601 LIILSVPGEFTTMAGRRLREAVKETLISNGNGEFDDDTHVVIAGLTNAYSQYVATSEEYE 660

Query: 661 QQRYEAASTLYGPHTLSAYIQEFKKLATAIAKGENIVKPIASPPDLSSVQLRFVLEPFGE 720
           QQRYEAASTLYGPHTLSAYIQEFKKLA AIAKGE I KPI SPPDLSSVQLRFV +PF E
Sbjct: 661 QQRYEAASTLYGPHTLSAYIQEFKKLAIAIAKGEKIAKPIVSPPDLSSVQLRFVSDPFIE 720

Query: 721 SPPNGINFGDIQQDIKLPKTGWFKRGDKQKPTATFWTANPRFDLLTEGTYAVVERLEKQR 780
           SPPNG+NFGDIQQDIKLPK GWFKRG KQKPTA FW+ANPRFDLLTEGTYAVVERLEK+R
Sbjct: 721 SPPNGVNFGDIQQDIKLPKVGWFKRGSKQKPTARFWSANPRFDLLTEGTYAVVERLEKRR 764

Query: 781 WKPAYDDDDFSLLFKWTLDNNSFINSLATIEWDIPTDADPGVYRLRHFGSSKTTTNSSNI 835
           W P YDDDDFSL+FKW LDNN+F NSLATIEWDIP DA+PGVYRLRHFGSSK+  NS+NI
Sbjct: 781 WTPVYDDDDFSLVFKWALDNNTFTNSLATIEWDIPIDANPGVYRLRHFGSSKSAINSTNI 764

BLAST of Lag0004087 vs. NCBI nr
Match: KAG6599114.1 (Neutral ceramidase 2, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 1293.1 bits (3345), Expect = 0.0e+00
Identity = 657/851 (77.20%), Postives = 701/851 (82.37%), Query Frame = 0

Query: 1   MRRPLLKLIMGTVIAAASWCC-VNGDWLVGVGSFDMTGPAAQVNMMGYANMDQNTAGVHF 60
           MRR LLK++   V+AA S CC  +GDWLVGVGSFDMTGPAA+VNMMGYANMDQ+ AG+HF
Sbjct: 1   MRRQLLKVVATAVLAAVSCCCAADGDWLVGVGSFDMTGPAAEVNMMGYANMDQSAAGIHF 60

Query: 61  RLRARTFVVAKTVDGPRFAFVNLDAGMASQLVTIKVLERLKS---------------RFG 120
           RLRARTF+VAK+VDGPR AFVNLDAGMASQLVTIK+LERLK+               RFG
Sbjct: 61  RLRARTFIVAKSVDGPRIAFVNLDAGMASQLVTIKLLERLKASLTYLSKLKPRHFIQRFG 120

Query: 121 DLYTEENVAISGIHTHAGPGGYLQYLVYSITSLGFVQQSFDAIVNAIEQSIIQAHQSLKP 180
           +LYTEENVAISGIHTHAGPGGYLQYLVYSITSLGFVQQSFDAIVNAIEQSI+QAH SLKP
Sbjct: 121 NLYTEENVAISGIHTHAGPGGYLQYLVYSITSLGFVQQSFDAIVNAIEQSIVQAHGSLKP 180

Query: 181 GSIFINKGDVVNAGINRSPSAYLLNPQEERARYSTNVDTEMTVLKFVDGASGKSVGAFSW 240
           G I INKG+V NAGINRSPSAYL+NP EERARY TNVDTEM+++K V+G SG S+GAFSW
Sbjct: 181 GFILINKGEVENAGINRSPSAYLMNPPEERARYPTNVDTEMSLVKVVEGGSGVSLGAFSW 240

Query: 241 FPTHGTSMSRDNKLISGDNKGAAARFFEDWI-ASKRTKRSSNNNASDFNEMGREIFLSCQ 300
           FPTHGTSMSRDNKLISGDNKGAAARFFEDW+ AS R+ R++++N S              
Sbjct: 241 FPTHGTSMSRDNKLISGDNKGAAARFFEDWVAASNRSTRATSSNKS-------------- 300

Query: 301 DFNEMGREILLKLLSVGVGEKSASELGRNGGIGGSELIGVRSEGERNDGIGGSELVGVRS 360
                                                                       
Sbjct: 301 ------------------------------------------------------------ 360

Query: 361 GRETTASAATALVDIVELMKKAETIKATGGKRCSKASSQASKVRNNDGSLFVAAFCQSNV 420
                        DI+ELMKKAETIKATGGKRCSK SSQ+SKVR NDGSLFV AFCQSNV
Sbjct: 361 -------------DILELMKKAETIKATGGKRCSKTSSQSSKVRKNDGSLFVGAFCQSNV 420

Query: 421 GDVTPNVLGAFCTDSGNPCDFNRSSCHGNDLLCVGRGPGFPDEILSTKIIGERQFLKAAD 480
           GDVTPNVLGAFCTDSG PCDFNRSSCHG+DLLCVGRGPGFPDEILSTKIIGERQFLKA D
Sbjct: 421 GDVTPNVLGAFCTDSGKPCDFNRSSCHGSDLLCVGRGPGFPDEILSTKIIGERQFLKAVD 480

Query: 481 LFTTATEKLTGEIDFRHVYLNFTDIEVELDGDNVVRTCPAALGPGFAAGTTDGPGVFGFQ 540
           LFTTATE+LTGEIDFRHVYLNFTDIEVEL GDNVV+TCPAALGPGFAAGTTDGPGVFGFQ
Sbjct: 481 LFTTATERLTGEIDFRHVYLNFTDIEVELGGDNVVKTCPAALGPGFAAGTTDGPGVFGFQ 540

Query: 541 QGDTKINELWRKLRDSLRKPSEFQVGCQKPKTVLLDTGEMFEPYAWAPAILPIQILRLGK 600
           QGDT+IN+LW+KLRDSLRKPSEFQVGCQKPKTVLLDTGEMFEPYAWAPAILPIQILRLGK
Sbjct: 541 QGDTQINKLWKKLRDSLRKPSEFQVGCQKPKTVLLDTGEMFEPYAWAPAILPIQILRLGK 600

Query: 601 LIILSVPGEFTTMAGRRLREAVKETLISNGNGEFDDDTHVVIAGLTNTYSQYVATFEEYE 660
           LIILSVPGEFTTMAGRRLREAVKETLISNGNGEFDDDTHVVIAGLTN YSQYVAT EEYE
Sbjct: 601 LIILSVPGEFTTMAGRRLREAVKETLISNGNGEFDDDTHVVIAGLTNAYSQYVATSEEYE 660

Query: 661 QQRYEAASTLYGPHTLSAYIQEFKKLATAIAKGENIVKPIASPPDLSSVQLRFVLEPFGE 720
           QQRYEAASTLYGPHTLSAYIQEFKKLA AIAKGE I KPI SPPDLSSVQLRFV +PF E
Sbjct: 661 QQRYEAASTLYGPHTLSAYIQEFKKLAIAIAKGEKIAKPIVSPPDLSSVQLRFVSDPFIE 720

Query: 721 SPPNGINFGDIQQDIKLPKTGWFKRGDKQKPTATFWTANPRFDLLTEGTYAVVERLEKQR 780
           SPPNG+NFGDIQQDIKLPK GWFKRG KQKPTA FW+ANPRFDLLTEGTYAVVERLEK+R
Sbjct: 721 SPPNGVNFGDIQQDIKLPKVGWFKRGSKQKPTARFWSANPRFDLLTEGTYAVVERLEKRR 764

Query: 781 WKPAYDDDDFSLLFKWTLDNNSFINSLATIEWDIPTDADPGVYRLRHFGSSKTTTNSSNI 835
           W P YDDDDFSL+FKW LDNN+F NSLATIEWDIP DA+PGVYRLRHFGSSK+  NS+NI
Sbjct: 781 WTPVYDDDDFSLVFKWALDNNTFTNSLATIEWDIPIDANPGVYRLRHFGSSKSAINSTNI 764

BLAST of Lag0004087 vs. NCBI nr
Match: XP_008464893.1 (PREDICTED: neutral ceramidase-like [Cucumis melo])

HSP 1 Score: 1291.6 bits (3341), Expect = 0.0e+00
Identity = 652/834 (78.18%), Postives = 697/834 (83.57%), Query Frame = 0

Query: 10  MGTVIAAASWCCVNGDWLVGVGSFDMTGPAAQVNMMGYANMDQNTAGVHFRLRARTFVVA 69
           M  V+   SW CVNGDWLVGVGSFDMTGPAA+VNMMGYANMDQ TAG+HFRLRARTFVVA
Sbjct: 1   MAIVVLDVSW-CVNGDWLVGVGSFDMTGPAAEVNMMGYANMDQVTAGIHFRLRARTFVVA 60

Query: 70  KTVDGPRFAFVNLDAGMASQLVTIKVLERLKS---------RFGDLYTEENVAISGIHTH 129
           +++DGPR AFVNLDAGMASQLVTIKVLERL+S         RFGDLYTEENVAISGIHTH
Sbjct: 61  RSIDGPRIAFVNLDAGMASQLVTIKVLERLQSRRNINISVQRFGDLYTEENVAISGIHTH 120

Query: 130 AGPGGYLQYLVYSITSLGFVQQSFDAIVNAIEQSIIQAHQSLKPGSIFINKGDVVNAGIN 189
           AGPGGYLQYLVYSITSLGFVQQSFDAIVNAIEQSIIQAH+SLKPG I  NKG+V NAGIN
Sbjct: 121 AGPGGYLQYLVYSITSLGFVQQSFDAIVNAIEQSIIQAHESLKPGFILFNKGEVDNAGIN 180

Query: 190 RSPSAYLLNPQEERARYSTNVDTEMTVLKFVDGASGKSVGAFSWFPTHGTSMSRDNKLIS 249
           RSPSAYL+NP+EERA+Y+ NVD EM++LKFVDG SG S+GAFSWFPTHGTSMSRDNKLIS
Sbjct: 181 RSPSAYLMNPEEERAKYANNVDKEMSILKFVDGESGNSIGAFSWFPTHGTSMSRDNKLIS 240

Query: 250 GDNKGAAARFFEDWIASKRTKRSSNNNASDFNEMGREIFLSCQDFNEMGREILLKLLSVG 309
           GDNKGAAARFFEDW AS                                           
Sbjct: 241 GDNKGAAARFFEDWAAS------------------------------------------- 300

Query: 310 VGEKSASELGRNGGIGGSELIGVRSEGERNDGIGGSELVGVRSGRETTASAATALVDIVE 369
                                                     +GR TT  +  A V I E
Sbjct: 301 ------------------------------------------NGRTTTTPSNNASV-IEE 360

Query: 370 LMKKAETIKATGGKRCSKASSQASKVRNNDGSLFVAAFCQSNVGDVTPNVLGAFCTDSGN 429
           LMKKAE IKATGGK+CSK SS++SKVR NDGSLFVAAFCQSNVGDVTPNVLGAFC DSG 
Sbjct: 361 LMKKAEKIKATGGKQCSKTSSKSSKVRKNDGSLFVAAFCQSNVGDVTPNVLGAFCIDSGK 420

Query: 430 PCDFNRSSCHGNDLLCVGRGPGFPDEILSTKIIGERQFLKAADLFTTATEKLTGEIDFRH 489
           PCDFNRSSCHG+DLLCVGRGPGFPDEILSTKIIGERQFLKAADLFTTATEKLTGEIDFRH
Sbjct: 421 PCDFNRSSCHGSDLLCVGRGPGFPDEILSTKIIGERQFLKAADLFTTATEKLTGEIDFRH 480

Query: 490 VYLNFTDIEVELDGDNVVRTCPAALGPGFAAGTTDGPGVFGFQQGDTKINELWRKLRDSL 549
           VYLNFTDIEVE+DG+NVVRTCPAALGPGFAAGTTDGPGVFGFQQGDT+IN+LW++LRDSL
Sbjct: 481 VYLNFTDIEVEIDGNNVVRTCPAALGPGFAAGTTDGPGVFGFQQGDTEINKLWKQLRDSL 540

Query: 550 RKPSEFQVGCQKPKTVLLDTGEMFEPYAWAPAILPIQILRLGKLIILSVPGEFTTMAGRR 609
           +KPSEFQVGCQKPKTVLLDTGEMFEPYAWAPAILPIQILRLGKLIILSVPGEFTTMAGRR
Sbjct: 541 KKPSEFQVGCQKPKTVLLDTGEMFEPYAWAPAILPIQILRLGKLIILSVPGEFTTMAGRR 600

Query: 610 LREAVKETLISNGNGEFDDDTHVVIAGLTNTYSQYVATFEEYEQQRYEAASTLYGPHTLS 669
           LREAVKETLISNGNGEFDDDTHVVIAGLTNTYSQYVATFEEYEQQRYEAASTLYGPHTLS
Sbjct: 601 LREAVKETLISNGNGEFDDDTHVVIAGLTNTYSQYVATFEEYEQQRYEAASTLYGPHTLS 660

Query: 670 AYIQEFKKLATAIAKGENIVKPIASPPDLSSVQLRFVLEPFGESPPNGINFGDIQQDIKL 729
           AYIQEFKKLA+AIAKGE + KPIASPPDLSSVQLR V++PFGESPP+G+NFGDIQQD++L
Sbjct: 661 AYIQEFKKLASAIAKGEKVAKPIASPPDLSSVQLRLVMDPFGESPPDGVNFGDIQQDVEL 720

Query: 730 PKTGWFKRGDKQKPTATFWTANPRFDLLTEGTYAVVERLEKQRWKPAYDDDDFSLLFKWT 789
           PK GWFK G KQKPTATFW+ANPRFDLLTEGTYA+VERLEKQRW PAYDDDDFS+ FKW 
Sbjct: 721 PKDGWFKTGSKQKPTATFWSANPRFDLLTEGTYALVERLEKQRWTPAYDDDDFSVSFKWK 747

Query: 790 LDNNSFINSLATIEWDIPTDADPGVYRLRHFGSSKTTTNSSNIYFSGASRAFAV 835
           LDN + INSLATIEWDIP DADPGVYRLRHFGSS++T NS+NIYF+GASRAFAV
Sbjct: 781 LDNTTLINSLATIEWDIPIDADPGVYRLRHFGSSRSTINSTNIYFTGASRAFAV 747

BLAST of Lag0004087 vs. NCBI nr
Match: XP_031742885.1 (neutral ceramidase 2 [Cucumis sativus])

HSP 1 Score: 1289.2 bits (3335), Expect = 0.0e+00
Identity = 650/831 (78.22%), Postives = 693/831 (83.39%), Query Frame = 0

Query: 10  MGTVIAAASWCCVNGDWLVGVGSFDMTGPAAQVNMMGYANMDQNTAGVHFRLRARTFVVA 69
           M TV+ A SW CVNGDWLVGVGSFDMTGPAA+VNMMGYANMDQNTAG+HFRLRARTFVVA
Sbjct: 1   MATVVVAVSW-CVNGDWLVGVGSFDMTGPAAEVNMMGYANMDQNTAGIHFRLRARTFVVA 60

Query: 70  KTVDGPRFAFVNLDAGMASQLVTIKVLERLKS-----RFGDLYTEENVAISGIHTHAGPG 129
            +VDGPR AFVNLDAGMASQLVTIKVLERLKS     RFGD+YTEENVAISGIHTHAGPG
Sbjct: 61  SSVDGPRIAFVNLDAGMASQLVTIKVLERLKSSILVQRFGDVYTEENVAISGIHTHAGPG 120

Query: 130 GYLQYLVYSITSLGFVQQSFDAIVNAIEQSIIQAHQSLKPGSIFINKGDVVNAGINRSPS 189
           GYLQYLVYSITSLGFVQQSFDAIVNAIEQSIIQAH+SLKPG I  NKG+V NAGINRSPS
Sbjct: 121 GYLQYLVYSITSLGFVQQSFDAIVNAIEQSIIQAHESLKPGFILFNKGEVENAGINRSPS 180

Query: 190 AYLLNPQEERARYSTNVDTEMTVLKFVDGASGKSVGAFSWFPTHGTSMSRDNKLISGDNK 249
           AYL+NP+EERA+Y+ NVD EM++LKFVDG +G S+GAFSWFPTHGTSMSRDNKLISGDNK
Sbjct: 181 AYLMNPEEERAKYANNVDKEMSILKFVDGENGNSIGAFSWFPTHGTSMSRDNKLISGDNK 240

Query: 250 GAAARFFEDWIAS-KRTKRSSNNNASDFNEMGREIFLSCQDFNEMGREILLKLLSVGVGE 309
           GAAARFFEDW AS +RT  + +NN S                                  
Sbjct: 241 GAAARFFEDWAASNRRTTTTPSNNTS---------------------------------- 300

Query: 310 KSASELGRNGGIGGSELIGVRSEGERNDGIGGSELVGVRSGRETTASAATALVDIVELMK 369
                                                                 I +LMK
Sbjct: 301 -----------------------------------------------------GIEDLMK 360

Query: 370 KAETIKATGGKRCSKASSQASKVRNNDGSLFVAAFCQSNVGDVTPNVLGAFCTDSGNPCD 429
           KA  IKATGGKRCSK SS++SKVR NDGSLFV AFCQSNVGDVTPNVLGAFCTDSGNPCD
Sbjct: 361 KAGKIKATGGKRCSKTSSKSSKVRKNDGSLFVGAFCQSNVGDVTPNVLGAFCTDSGNPCD 420

Query: 430 FNRSSCHGNDLLCVGRGPGFPDEILSTKIIGERQFLKAADLFTTATEKLTGEIDFRHVYL 489
           FN SSCHG+DLLCVGRGPGFPDEILSTKIIGERQFLKAADLFTTATEKLTGEIDFRHVYL
Sbjct: 421 FNHSSCHGSDLLCVGRGPGFPDEILSTKIIGERQFLKAADLFTTATEKLTGEIDFRHVYL 480

Query: 490 NFTDIEVELDGDNVVRTCPAALGPGFAAGTTDGPGVFGFQQGDTKINELWRKLRDSLRKP 549
           NFTDIEV +DG+NVVRTCPAALGPGFAAGTTDGPG FGFQQGDT+IN+LW++LRDSL+KP
Sbjct: 481 NFTDIEVAIDGNNVVRTCPAALGPGFAAGTTDGPGAFGFQQGDTEINKLWKQLRDSLKKP 540

Query: 550 SEFQVGCQKPKTVLLDTGEMFEPYAWAPAILPIQILRLGKLIILSVPGEFTTMAGRRLRE 609
           SEFQVGCQKPKTVLLDTGEMFEPYAWAPAILPIQILRLG LIILSVPGEFTTMAGRRLRE
Sbjct: 541 SEFQVGCQKPKTVLLDTGEMFEPYAWAPAILPIQILRLGNLIILSVPGEFTTMAGRRLRE 600

Query: 610 AVKETLISNGNGEFDDDTHVVIAGLTNTYSQYVATFEEYEQQRYEAASTLYGPHTLSAYI 669
           AVKETLISNGNG FDDDTHVVIAGLTNTYSQYVATFEEYEQQRYEAASTLYGPHTLSAYI
Sbjct: 601 AVKETLISNGNGVFDDDTHVVIAGLTNTYSQYVATFEEYEQQRYEAASTLYGPHTLSAYI 660

Query: 670 QEFKKLATAIAKGENIVKPIASPPDLSSVQLRFVLEPFGESPPNGINFGDIQQDIKLPKT 729
           QEFKKLA AIAKGE I KPIASPPDLSSVQLR V++PFGESPPNG+NFGDIQQD++LPK 
Sbjct: 661 QEFKKLAIAIAKGEKIAKPIASPPDLSSVQLRLVMDPFGESPPNGVNFGDIQQDVELPKD 720

Query: 730 GWFKRGDKQKPTATFWTANPRFDLLTEGTYAVVERLEKQRWKPAYDDDDFSLLFKWTLDN 789
           GWFK G KQKPTATFW+ANPRFDLLTEGTYA+VERLEKQRW PAYDDDDFSL FKW LD 
Sbjct: 721 GWFKTGSKQKPTATFWSANPRFDLLTEGTYALVERLEKQRWTPAYDDDDFSLFFKWKLD- 742

Query: 790 NSFINSLATIEWDIPTDADPGVYRLRHFGSSKTTTNSSNIYFSGASRAFAV 835
           N+FIN+L TIEWDIP DADPGVYRLRHFGSS++T NS+N+YF+GASRAFAV
Sbjct: 781 NTFINNLVTIEWDIPIDADPGVYRLRHFGSSRSTINSTNVYFTGASRAFAV 742

BLAST of Lag0004087 vs. ExPASy Swiss-Prot
Match: Q304B9 (Neutral ceramidase 2 OS=Arabidopsis thaliana OX=3702 GN=NCER2 PE=3 SV=1)

HSP 1 Score: 837.0 bits (2161), Expect = 1.8e-241
Identity = 443/815 (54.36%), Postives = 553/815 (67.85%), Query Frame = 0

Query: 26  WLVGVGSFDMTGPAAQVNMMGYANMDQNTAGVHFRLRARTFVVAKTVDGPRFAFVNLDAG 85
           +L+GVGS+D+TGPAA VNMMGYAN DQ  +G+HFRLRAR F+VA+   G R  FVNLDA 
Sbjct: 26  YLIGVGSYDITGPAADVNMMGYANSDQIASGIHFRLRARAFIVAEP-QGNRVVFVNLDAC 85

Query: 86  MASQLVTIKVLERLKSRFGDLYTEENVAISGIHTHAGPGGYLQYLVYSITSLGFVQQSFD 145
           MASQ+VTIKVLERLK+R+G+LYTE+NVAISGIHTHAGPGGYLQY+ Y +TSLGFV+QSFD
Sbjct: 86  MASQIVTIKVLERLKARYGELYTEKNVAISGIHTHAGPGGYLQYVTYIVTSLGFVRQSFD 145

Query: 146 AIVNAIEQSIIQAHQSLKPGSIFINKGDVVNAGINRSPSAYLLNPQEERARYSTNVDTEM 205
            +VN IEQSI+QAH+SL+PGS F+NKGD+++AG+NRSPS+YL NP  ER++Y  +VD EM
Sbjct: 146 VVVNGIEQSIVQAHESLRPGSAFVNKGDLLDAGVNRSPSSYLNNPAAERSKYKYDVDKEM 205

Query: 206 TVLKFVDGASGKSVGAFSWFPTHGTSMSRDNKLISGDNKGAAARFFEDWIASKRTKRSSN 265
           T++KFVD   G + G+F+WF THGTSMSR N LISGDNKGAAARF EDW  + +    S+
Sbjct: 206 TLVKFVDSQLGPT-GSFNWFATHGTSMSRTNSLISGDNKGAAARFMEDWFENGQKNSVSS 265

Query: 266 NNASDFNEMGREIFLSCQDFNEMGREILLKLLSVGVGEKSASELGRNGGIGGSELIGVRS 325
            N      + R +     DF+   R+ LL +                             
Sbjct: 266 RN------IPRRVSTIVSDFSR-NRDRLLDI----------------------------- 325

Query: 326 EGERNDGIGGSELVGVRSGRETTASAATALVDIVELMKKAETIKATGGKRCSKASSQASK 385
                                                  A T K++ G    K+    ++
Sbjct: 326 ---------------------------------------AATYKSSRGHSVDKSLDVKTR 385

Query: 386 VRNNDGSLFVAAFCQSNVGDVTPNVLGAFCTDSGNPCDFNRSSCHGNDLLCVGRGPGFPD 445
           VRN     FV+AFCQSN GDV+PN LG FC D+G PCDFN S+C+G + LC GRGPG+PD
Sbjct: 386 VRNGSKRKFVSAFCQSNCGDVSPNTLGTFCIDTGLPCDFNHSTCNGQNELCYGRGPGYPD 445

Query: 446 EILSTKIIGERQFLKAADLFTTATEKLTGEIDFRHVYLNFTDIEVEL----DGDNVVRTC 505
           E  ST+IIGE+QF  A +LF  ATEKL G+I ++H YL+F++++V +     G   V+TC
Sbjct: 446 EFESTRIIGEKQFKMAVELFNKATEKLQGKIGYQHAYLDFSNLDVTVPKAGGGSETVKTC 505

Query: 506 PAALGPGFAAGTTDGPGVFGFQQGDTKINELWRKLRDSLRKPSEFQVGCQKPKTVLLDTG 565
           PAA+G GFAAGTTDGPG F F+QGD + N  WR +R+ LR P   QV CQKPK +LLDTG
Sbjct: 506 PAAMGFGFAAGTTDGPGAFDFKQGDDQGNVFWRLVRNVLRTPGPEQVQCQKPKPILLDTG 565

Query: 566 EMFEPYAWAPAILPIQILRLGKLIILSVPGEFTTMAGRRLREAVKETLISNGNGEFDDDT 625
           EM EPY WAP+ILPIQILR+G+L+ILSVPGEFTTMAGRRLR+A+K  LIS+   EF ++ 
Sbjct: 566 EMKEPYDWAPSILPIQILRIGQLVILSVPGEFTTMAGRRLRDAIKSFLISSDPKEFSNNM 625

Query: 626 HVVIAGLTNTYSQYVATFEEYEQQRYEAASTLYGPHTLSAYIQEFKKLATAIAKGENIVK 685
           HVVIAGLTNTYSQY+ATFEEYE QRYE ASTLYG HTL+AYIQEFKKLATA+  G  + +
Sbjct: 626 HVVIAGLTNTYSQYIATFEEYEVQRYEGASTLYGRHTLTAYIQEFKKLATALVNGLTLPR 685

Query: 686 PIASPPDLSSVQLRFVLEPFGESPPNGINFGDIQQDIKLPKTGWFKRGDKQKPTATFWTA 745
               PPDL   Q+  +     +S P G+ FGD++ D+  P    F+RG  Q+  ATFW+ 
Sbjct: 686 G-PQPPDLLDKQISLLSPVVVDSTPLGVKFGDVKADV--PPKSTFRRG--QQVNATFWSG 745

Query: 746 NPRFDLLTEGTYAVVERL-EKQRWKPAYDDDDFSLLFKWTLDNNSFINSLATIEWDIPTD 805
            PR DL+TEG++AVVE L E  +W P YDDDDFSL FKW+        S ATIEW +P  
Sbjct: 746 CPRNDLMTEGSFAVVETLREGGKWAPVYDDDDFSLKFKWSRPAKLSSESQATIEWRVPES 757

Query: 806 ADPGVYRLRHFGSSKTTTNSSNIYFSGASRAFAVM 836
           A  GVYR+RH+G+SK+   S +  FSG+S AF V+
Sbjct: 806 AVAGVYRIRHYGASKSLFGSIS-SFSGSSSAFVVV 757

BLAST of Lag0004087 vs. ExPASy Swiss-Prot
Match: Q0JL46 (Neutral ceramidase OS=Oryza sativa subsp. japonica OX=39947 GN=Os01g0624000 PE=1 SV=1)

HSP 1 Score: 828.6 bits (2139), Expect = 6.5e-239
Identity = 440/840 (52.38%), Postives = 571/840 (67.98%), Query Frame = 0

Query: 3   RPLLKLIMGTVIAAASWCCVNGDWLVGVGSFDMTGPAAQVNMMGYANMDQNTAGVHFRLR 62
           R  L L++  V+   S       +LVG+GSFD+TGPAA VNMMGYAN +Q  +G+HFRL+
Sbjct: 18  RVWLWLLLALVLLNCSLVLSASPYLVGMGSFDITGPAADVNMMGYANTEQIASGIHFRLK 77

Query: 63  ARTFVVAKTVDGPRFAFVNLDAGMASQLVTIKVLERLKSRFGDLYTEENVAISGIHTHAG 122
           +R F+VA+  +G R  FVN+DA MASQ+VTIKVLERLK+R+GDLY E NVAISGIHTHAG
Sbjct: 78  SRAFIVAEP-NGKRVVFVNIDACMASQIVTIKVLERLKARYGDLYNENNVAISGIHTHAG 137

Query: 123 PGGYLQYLVYSITSLGFVQQSFDAIVNAIEQSIIQAHQSLKPGSIFINKGDVVNAGINRS 182
           PGGYLQY+VY +TSLGFV+QSFD IV+ IEQSI++AH +L+PG IF+NKGD+++AG+NRS
Sbjct: 138 PGGYLQYVVYIVTSLGFVRQSFDVIVDGIEQSIVEAHNNLRPGKIFVNKGDLLDAGVNRS 197

Query: 183 PSAYLLNPQEERARYSTNVDTEMTVLKFVDGASGKSVGAFSWFPTHGTSMSRDNKLISGD 242
           PSAYL NP EER++Y  NVD EMT++KFVD   G  VG+F+WF THGTSMSR N LISGD
Sbjct: 198 PSAYLNNPAEERSKYEYNVDKEMTLIKFVDDELG-PVGSFNWFATHGTSMSRTNSLISGD 257

Query: 243 NKGAAARFFEDWIASK-RTKRSSNNNASDFNEMGREIFLSCQDFNEMGREILLKLLSVGV 302
           NKGAAARF EDW       K+S++ N+ D   + +               +L + +S  +
Sbjct: 258 NKGAAARFMEDWAEQMGLPKQSAHANSDDLRSLHK-------------TSVLPRRVSTII 317

Query: 303 GEKSASELGRNGGIGGSELIGVRSEGERNDGIGGSELVGVRSGRETTASAATALVDIVEL 362
            E +                      E  D                            +L
Sbjct: 318 PEPN----------------------EITD----------------------------DL 377

Query: 363 MKKAETIKATGGKRCSKASSQASKVRNNDGS--LFVAAFCQSNVGDVTPNVLGAFCTDSG 422
           ++ A + +A+GG+R +  SS   ++R+   +   FV+AFCQSN GDV+PNVLG FC D+ 
Sbjct: 378 IQLASSYEASGGRRLA-GSSITRRIRSTQQNKPKFVSAFCQSNCGDVSPNVLGTFCIDTN 437

Query: 423 NPCDFNRSSCHGNDLLCVGRGPGFPDEILSTKIIGERQFLKAADLFTTATEKLTGEIDFR 482
            PCDFN S+C+G + LC GRGPG+PDE  ST++IG RQFLKA DLF +A+E++ G+ID+R
Sbjct: 438 LPCDFNHSTCNGKNELCYGRGPGYPDEFESTRVIGNRQFLKARDLFDSASEEIQGKIDYR 497

Query: 483 HVYLNFTDIEVELD----GDNVVRTCPAALGPGFAAGTTDGPGVFGFQQGDTKINELWRK 542
           H YL+F+ +EV++     G   V+TCPAA+G  FAAGTTDGPG F F+QGD K N  W+ 
Sbjct: 498 HTYLDFSKLEVKVSTSAGGQQTVKTCPAAMGFAFAAGTTDGPGAFDFRQGDVKGNPFWKL 557

Query: 543 LRDSLRKPSEFQVGCQKPKTVLLDTGEMFEPYAWAPAILPIQILRLGKLIILSVPGEFTT 602
           +R+ L+ P + QV C  PK +LLDTGEM EPY WAPAILP+Q++R+G+L+IL VPGEFTT
Sbjct: 558 VRNLLKTPGKDQVECHSPKPILLDTGEMKEPYDWAPAILPVQMIRIGQLVILCVPGEFTT 617

Query: 603 MAGRRLREAVKETLISNGNGEFDDDTHVVIAGLTNTYSQYVATFEEYEQQRYEAASTLYG 662
           MAGRRLR+AVK T++++GN EFD + HVV+AGLTN+YSQY+ TFEEY+ QRYE ASTLYG
Sbjct: 618 MAGRRLRDAVK-TVLTSGNSEFDKNIHVVLAGLTNSYSQYITTFEEYQIQRYEGASTLYG 677

Query: 663 PHTLSAYIQEFKKLATAIAKGENIVKPIASPPDLSSVQLRFVLEPFGESPPNGINFGDIQ 722
           PHTLSAYIQEF+KLA A+   + +      PPD+   Q+  +     +S P G+ FGD+ 
Sbjct: 678 PHTLSAYIQEFQKLAMAMIANKEVPTNF-QPPDMLDKQIGLLPGVVFDSTPLGVKFGDVN 737

Query: 723 QDIKLPKTGWFKRGDKQKPTATFWTANPRFDLLTEGTYAVVERLE-KQRWKPAYDDDDFS 782
            D+  P    F +G      ATF++A PR DLLT+GT+A+VE+L+    W P YDDDD+S
Sbjct: 738 SDV--PGNSTFNKG--STVNATFYSACPRNDLLTDGTFALVEKLDGNNNWVPVYDDDDWS 784

Query: 783 LLFKWTLDNNSFINSLATIEWDIPTDADPGVYRLRHFGSSKTTTNSSNIYFSGASRAFAV 835
           L FKW+        S AT+EW +P DA  GVYRLRHFG+SK    S   +F+G SRAFAV
Sbjct: 798 LRFKWSRPARLSSRSFATLEWTVPEDAAAGVYRLRHFGASKPMFGSVR-HFTGTSRAFAV 784

BLAST of Lag0004087 vs. ExPASy Swiss-Prot
Match: F4HQM3 (Neutral ceramidase 1 OS=Arabidopsis thaliana OX=3702 GN=NCER1 PE=2 SV=1)

HSP 1 Score: 806.6 bits (2082), Expect = 2.6e-232
Identity = 434/822 (52.80%), Postives = 555/822 (67.52%), Query Frame = 0

Query: 23  NGDWLVGVGSFDMTGPAAQVNMMGYANMDQNTAGVHFRLRARTFVVAKTVDGPRFAFVNL 82
           + ++L+G+GS+D+TGPAA VNMMGYANM+Q  +G+HFRLRARTF+V++   G R  FVNL
Sbjct: 34  HSEYLIGLGSYDITGPAADVNMMGYANMEQVASGIHFRLRARTFIVSEP-QGKRVVFVNL 93

Query: 83  DAGMASQLVTIKVLERLKSRFGDLYTEENVAISGIHTHAGPGGYLQYLVYSITSLGFVQQ 142
           DA MASQ+V +KV+ERLK+R+GDLYTE+NV ISGIHTHAGPGGYLQY+VY +TSLGFV+Q
Sbjct: 94  DACMASQIVKLKVIERLKARYGDLYTEQNVGISGIHTHAGPGGYLQYVVYIVTSLGFVRQ 153

Query: 143 SFDAIVNAIEQSIIQAHQSLKPGSIFINKGDVVNAGINRSPSAYLLNPQEERARYSTNVD 202
           SFDA+V+ IE SIIQAH++L+PGSIF+N G++++AG+NRSPSAYL NP +ER+++  NVD
Sbjct: 154 SFDALVDGIENSIIQAHENLRPGSIFLNNGELLDAGVNRSPSAYLNNPSKERSKHKYNVD 213

Query: 203 TEMTVLKFVDGASGKSVGAFSWFPTHGTSMSRDNKLISGDNKGAAARFFEDWIASKRTKR 262
            EMT+LKFVD   G  VG+F+WF THGTSMSR N LISGDNKGAA+RF EDW      +R
Sbjct: 214 KEMTLLKFVDDQWG-PVGSFNWFATHGTSMSRTNSLISGDNKGAASRFMEDWYEQNTAER 273

Query: 263 SSNNNASDFNEMGREIFLSCQDFNEMGREILLKLLSVGVGEKSASELGRNGGIGGSELIG 322
           S +           E F+S    +E+ R +                         S LI 
Sbjct: 274 SYS-----------EEFIS----DEIPRRV-------------------------SSLIE 333

Query: 323 VRSEGERNDGIGGSELVGVRSGRETTASAATALVDIVELMKKAETIKATGGKRCSKASSQ 382
              +                                 EL++ A   ++  GK  ++ SS 
Sbjct: 334 NHQDSHH------------------------------ELLELASYFESQPGKPVTRISSS 393

Query: 383 ASKVRN----NDGSLFVAAFCQSNVGDVTPNVLGAFCTDSGNPCDFNRSSCHGNDLLCVG 442
           A +VR+     D   FV+AFCQ+N GDV+PNVLGAFC D+G PCDFN S+C G + +C G
Sbjct: 394 ARRVRSALRKADKPGFVSAFCQTNCGDVSPNVLGAFCLDTGLPCDFNHSTCGGKNEMCYG 453

Query: 443 RGPGFPDEILSTKIIGERQFLKAADLFTTATEKLTGEIDFRHVYLNFTDIEVEL---DG- 502
           RGPG+PDE  ST+IIGERQF  A +LF  A+E+L G++D+RHVY++F+ + V L   DG 
Sbjct: 454 RGPGYPDEFESTRIIGERQFKMALELFNKASEQLQGKVDYRHVYVDFSQLNVTLPKKDGK 513

Query: 503 DNVVRTCPAALGPGFAAGTTDGPGVFGFQQGDTKINELWRKLRDSLRKPSEFQVGCQKPK 562
             VV+TCPAA+G  FAAGTTDGPG F F QGD K N  WR +R+ L+ P + Q+ C  PK
Sbjct: 514 SEVVKTCPAAMGFAFAAGTTDGPGAFDFTQGDDKGNPFWRLVRNVLKTPDKKQIDCHYPK 573

Query: 563 TVLLDTGEMFEPYAWAPAILPIQILRLGKLIILSVPGEFTTMAGRRLREAVKETLISNGN 622
            +LLDTGEM +PY WAP+IL +Q+LR+G+L ILSVPGEFTTMAGRRLR AVK  L ++GN
Sbjct: 574 PILLDTGEMTKPYDWAPSILSLQVLRIGQLFILSVPGEFTTMAGRRLRYAVKTQLKNSGN 633

Query: 623 GEFDDDTHVVIAGLTNTYSQYVATFEEYEQQRYEAASTLYGPHTLSAYIQEFKKLATAIA 682
            +   + HVVIAGL N YSQYV TFEEY+ QRYE ASTLYGPHTLS YIQEFKKL+ ++ 
Sbjct: 634 KDLSGEIHVVIAGLANGYSQYVTTFEEYQVQRYEGASTLYGPHTLSGYIQEFKKLSKSLV 693

Query: 683 KGENIVKPIASPPDLSSVQLRFVLEPFGESPPNGINFGDIQQDIKLPKTGWFKRGDKQKP 742
             +  V+P   PPDL   QL F+     ++ P+G +FGD+  D+  PK    KRG+ Q  
Sbjct: 694 L-DMPVQPGPQPPDLLDKQLSFLTPVMMDTTPSGDSFGDVISDV--PKNLSLKRGNGQ-V 753

Query: 743 TATFWTANPRFDLLTEGTYAVVERLEK--QRWKPAYDDDDFSLLFKWTLDNNSFINSLAT 802
           T  F +A PR DLLTEGT+ +VERLE+  + W P YDDDD  L FKW+        S AT
Sbjct: 754 TVVFRSACPRNDLLTEGTFTLVERLEQKDKTWTPVYDDDDLCLRFKWSRHKKLSSRSQAT 778

Query: 803 IEWDIPTDADPGVYRLRHFGSSKTTTNSSNIYFSGASRAFAV 835
           +EW IP  A PGVYR+ HFG++K    S + +F+G+S AF V
Sbjct: 814 VEWRIPESASPGVYRITHFGAAKKLFGSVH-HFTGSSSAFVV 778

BLAST of Lag0004087 vs. ExPASy Swiss-Prot
Match: F4KHQ8 (Neutral ceramidase 3 OS=Arabidopsis thaliana OX=3702 GN=NCER3 PE=2 SV=1)

HSP 1 Score: 765.0 bits (1974), Expect = 8.8e-220
Identity = 420/822 (51.09%), Postives = 531/822 (64.60%), Query Frame = 0

Query: 23  NGDWLVGVGSFDMTGPAAQVNMMGYANMDQNTAGVHFRLRARTFVVAKTVDGPRFAFVNL 82
           + D+L+G+GS+D+TGPAA VNMMGYANM+Q  +GVHFRLRAR F+VA+     R AFVNL
Sbjct: 26  DSDYLMGLGSYDITGPAADVNMMGYANMEQVASGVHFRLRARAFIVAEPYK-KRIAFVNL 85

Query: 83  DAGMASQLVTIKVLERLKSRFGDLYTEENVAISGIHTHAGPGGYLQYLVYSITSLGFVQQ 142
           DAGMASQLVTIKV+ERLK R+G+LYTEENVAISG HTHAGPGGYLQY++Y +TSLGFV Q
Sbjct: 86  DAGMASQLVTIKVIERLKQRYGELYTEENVAISGTHTHAGPGGYLQYILYLVTSLGFVHQ 145

Query: 143 SFDAIVNAIEQSIIQAHQSLKPGSIFINKGDVVNAGINRSPSAYLLNPQEERARYSTNVD 202
           SF+A+V+ IEQSIIQAH++L+PGSI INKG++++AG+NRSPSAYL NP  ER++Y  +VD
Sbjct: 146 SFNALVDGIEQSIIQAHENLRPGSILINKGELLDAGVNRSPSAYLNNPAHERSKYEYDVD 205

Query: 203 TEMTVLKFVDGASGKSVGAFSWFPTHGTSMSRDNKLISGDNKGAAARFFEDWIASKRTKR 262
            EMT++KFVD                             D  G  AR  EDW        
Sbjct: 206 KEMTLVKFVD-----------------------------DQWGPVARIMEDWF------- 265

Query: 263 SSNNNASDFNEMGREIFLSCQDFNEMGREILLKLLSVGVGEKSASELGRNGGIGGSELIG 322
                                                                       
Sbjct: 266 ------------------------------------------------------------ 325

Query: 323 VRSEGERNDGIGGSELVGVRSGRETTASAATALVDIVELMKKAETIKATGGKRCSKASSQ 382
                ER +   G   V V S R  ++  +       +LM+ A ++ +TGGK  ++ SS 
Sbjct: 326 -----EREN---GCRSVDVESPRRVSSIISDPYDQ--DLMEMASSLLSTGGKTVTRMSSV 385

Query: 383 ASKVRNN----DGSLFVAAFCQSNVGDVTPNVLGAFCTDSGNPCDFNRSSCHGNDLLCVG 442
           A +VR+     D   FV+AFCQ+N GDV+PNVLGAFC D+G PC+FN+S+C G +  C G
Sbjct: 386 ARRVRSRFRHADKPRFVSAFCQTNCGDVSPNVLGAFCIDTGLPCEFNQSTCGGKNEQCYG 445

Query: 443 RGPGFPDEILSTKIIGERQFLKAADLFTTATEKLTGEIDFRHVYLNFTDIEVELDGDN-- 502
           RGPG+PDE  ST+IIGERQF KAADLFT A+E++ G++D+RH Y++F+ +EV ++G N  
Sbjct: 446 RGPGYPDEFESTRIIGERQFKKAADLFTKASEEIQGKVDYRHAYVDFSQLEVTINGQNGG 505

Query: 503 --VVRTCPAALGPGFAAGTTDGPGVFGFQQGDTKINELWRKLRDSLRKPSEFQVGCQKPK 562
             VV+TCPAA+G GFAAGTTDGPG F F+QGD + N  WR +R+ L+ P+E QV CQ+PK
Sbjct: 506 SEVVKTCPAAMGFGFAAGTTDGPGAFDFKQGDDQGNPFWRLVRNLLKNPTEEQVRCQRPK 565

Query: 563 TVLLDTGEMFEPYAWAPAILPIQILRLGKLIILSVPGEFTTMAGRRLREAVKETLISNGN 622
            +LLDTGEM +PY WAP+ILP+QILR+G+L+IL VPGEFTTMAGRRLR+AVK  L    N
Sbjct: 566 PILLDTGEMKQPYDWAPSILPVQILRIGQLVILCVPGEFTTMAGRRLRDAVKTVLKEGSN 625

Query: 623 GEFDDDTHVVIAGLTNTYSQYVATFEEYEQQRYEAASTLYGPHTLSAYIQEFKKLATAIA 682
           G    +  VVIAGLTN+YSQY+ATFEEY+ QRYE ASTLYGPHTLS YIQEFKKLA  + 
Sbjct: 626 GR---EFSVVIAGLTNSYSQYIATFEEYQVQRYEGASTLYGPHTLSGYIQEFKKLANDLL 685

Query: 683 KGENIVKPIASPPDLSSVQLRFVLEPFGESPPNGINFGDIQQDIKLPKTGWFKRGDKQKP 742
             +    P   PPDL   Q+  +     +  P G  FGD+  D+  P+   F++G     
Sbjct: 686 SAQT-TDPGPQPPDLLHKQISLLTPVVADMTPIGTAFGDVTSDV--PRLSKFRKG-ADIV 732

Query: 743 TATFWTANPRFDLLTEGTYAVVER-LE-KQRWKPAYDDDDFSLLFKWTLDNNSFINSLAT 802
              F +ANPR DL+TEGT+A+VER LE ++ W P YDDDDF L FKW+        S AT
Sbjct: 746 RVQFRSANPRNDLMTEGTFALVERWLEGRETWVPVYDDDDFCLRFKWSRPFKLSTQSTAT 732

Query: 803 IEWDIPTDADPGVYRLRHFGSSKTTTNSSNIYFSGASRAFAV 835
           IEW IP  A PGVYR+ HFGS+KT  +S + +FSG+S AF V
Sbjct: 806 IEWRIPETASPGVYRITHFGSAKTPISSIH-HFSGSSSAFVV 732

BLAST of Lag0004087 vs. ExPASy Swiss-Prot
Match: Q55G11 (Neutral ceramidase B OS=Dictyostelium discoideum OX=44689 GN=dcd2B PE=3 SV=1)

HSP 1 Score: 504.2 bits (1297), Expect = 2.8e-141
Identity = 310/821 (37.76%), Postives = 435/821 (52.98%), Query Frame = 0

Query: 23  NGDWLVGVGSFDMTGPAAQVNMMGYANMDQNTAGVHFRLRARTFVVAKTVDGPRFAFVNL 82
           +  + +G G +D+TG +A+VN+MGYAN  Q  AG+HFR RAR FV   + +G R  +V+ 
Sbjct: 43  DSSYQIGAGIYDITGASAEVNLMGYANPLQVGAGIHFRQRARAFVFVDS-NGNRAVYVST 102

Query: 83  DAGMASQLVTIKVLERLKSRFG-DLYTEENVAISGIHTHAGPGGYLQYLVYSITSLGFVQ 142
           D+ M  Q V I V+E L+  FG ++YTE NV +SG HTH+GP G+ QY +Y ITSLGF +
Sbjct: 103 DSCMIFQEVKIHVVELLQDIFGPNVYTEANVLLSGTHTHSGPAGFSQYALYGITSLGFYK 162

Query: 143 QSFDAIVNAIEQSIIQAHQSLKPGSIFINKGDVVNAGINRSPSAYLLNPQEERARYSTNV 202
           ++FD I N I Q+I++AH+S++P ++F   G++ N  INRSP AY  NP+EE+A Y +NV
Sbjct: 163 KNFDTICNGIVQAIVKAHKSVQPANMFTETGELWNTNINRSPFAYDNNPEEEKAMYDSNV 222

Query: 203 DTEMTVLKFVDGASGKSVGAFSWFPTHGTSMSRDNKLISGDNKGAAARFFEDWIASKRTK 262
           D  MTVL+ ++  +G    A S+F  H TSM+  N LISGDNKG A+  +E  +      
Sbjct: 223 DKNMTVLR-IEDMNGNPFAAISFFAVHCTSMNNTNHLISGDNKGYASYLWEKQV------ 282

Query: 263 RSSNNNASDFNEMGREIFLSCQDFNEMGREILLKLLSVGVGEKSASELGRNGGIGGSELI 322
                                                             NG        
Sbjct: 283 --------------------------------------------------NG-------- 342

Query: 323 GVRSEGERNDGIGGSELVGVRSGRETTASAATALVDIVELMKKAETIKATGGKRCSKASS 382
                                                            T GK       
Sbjct: 343 -----------------------------------------------PGTAGK------- 402

Query: 383 QASKVRNNDGSLFVAAFCQSNVGDVTPNVLGAFCTDSGNPCDFNRSSCHGNDLLCVGRGP 442
                       FVAAF QSN GDV+PN  G  C D G+PCD+  S+C+G +  C   GP
Sbjct: 403 ----------GPFVAAFGQSNEGDVSPNTRGPTCRD-GSPCDYKTSTCNGRNEECWSLGP 462

Query: 443 GFPDEIL-STKIIGERQFLKAADLFTTATEKLTGEIDFRHVYLNFTDIEVE---LDGDNV 502
           G   ++  ST+IIG  QF KA +LF  A+ +++G + +RH ++ FT++ VE     G + 
Sbjct: 463 GKDGDMFESTQIIGGNQFNKALELFNNASIQVSGPVQYRHSWVQFTNVSVEPPYNSGVDN 522

Query: 503 VRTCPAALGPGFAAGTTDGPGVFGFQQGD--TKINELWRKLRDSLRKPSEFQVGCQKPKT 562
             TC  A+G  FAAGTTDGPG F F Q D  T  N  W  + D + KP+  Q+ CQ PK 
Sbjct: 523 ATTCRGAMGYSFAAGTTDGPGAFNFVQSDNNTSGNPFWNFIGDFIAKPTPDQIRCQSPKP 582

Query: 563 VLLDTGEMFEPYAWAPAILPIQILRLGKLIILSVPGEFTTMAGRRLREAVKETLISNGNG 622
           +LLD G M EP  W P ++PIQI+ +G++++++VPGEFTTM+GRRLR +V+E +     G
Sbjct: 583 ILLDVG-MVEPIPWVPDVMPIQIVTIGQIVLVAVPGEFTTMSGRRLRNSVREII-----G 642

Query: 623 EFDDDTHVVIAGLTNTYSQYVATFEEYEQQRYEAASTLYGPHTLSAYIQEFKKLATAIAK 682
           E  ++  V+IAGL+NTYS Y+ATFEEY+ QRYE AST++GPHTL +Y+QEF KLA +I  
Sbjct: 643 ESIENPIVLIAGLSNTYSGYIATFEEYQVQRYEGASTVFGPHTLGSYMQEFGKLAQSIVD 702

Query: 683 GENIVKPIASPPDLSSVQLRFVLEPFGESPPNGINFGDIQQDIKLPKTGWFKRGDKQKPT 742
           G   V    +P +L+   L F+     ++ P+  +FG++  D+ L           +  +
Sbjct: 703 G-TTVPAGPTPRNLTGHTLFFLPPVIVDAAPDFDDFGEVSIDVNL------NYSVNETVS 717

Query: 743 ATFWTANPRFDLLTEGTYAVVERLE-KQRWKPAYDDDDFSLLFKWTLDNNSFINSLATIE 802
             F+  NPR D + E ++  V+ L    +W    DD D+   FKW + +  F  SL TIE
Sbjct: 763 CVFYGGNPRNDFMIESSFLSVDLLTGTDQWTTVLDDGDWDTKFKWKMHDLGF--SLITIE 717

Query: 803 WDIPTDADPGVYRLRHFGSSKTTTNSSNIY-FSGASRAFAV 835
           W I  D  PG YR+ H G +K    SSN+  + G SR F V
Sbjct: 823 WVIAPDTTPGTYRITHSGFAKKNPFSSNLTPYQGISRNFVV 717

BLAST of Lag0004087 vs. ExPASy TrEMBL
Match: A0A1S3CMI6 (Neutral ceramidase OS=Cucumis melo OX=3656 GN=LOC103502652 PE=3 SV=1)

HSP 1 Score: 1291.6 bits (3341), Expect = 0.0e+00
Identity = 652/834 (78.18%), Postives = 697/834 (83.57%), Query Frame = 0

Query: 10  MGTVIAAASWCCVNGDWLVGVGSFDMTGPAAQVNMMGYANMDQNTAGVHFRLRARTFVVA 69
           M  V+   SW CVNGDWLVGVGSFDMTGPAA+VNMMGYANMDQ TAG+HFRLRARTFVVA
Sbjct: 1   MAIVVLDVSW-CVNGDWLVGVGSFDMTGPAAEVNMMGYANMDQVTAGIHFRLRARTFVVA 60

Query: 70  KTVDGPRFAFVNLDAGMASQLVTIKVLERLKS---------RFGDLYTEENVAISGIHTH 129
           +++DGPR AFVNLDAGMASQLVTIKVLERL+S         RFGDLYTEENVAISGIHTH
Sbjct: 61  RSIDGPRIAFVNLDAGMASQLVTIKVLERLQSRRNINISVQRFGDLYTEENVAISGIHTH 120

Query: 130 AGPGGYLQYLVYSITSLGFVQQSFDAIVNAIEQSIIQAHQSLKPGSIFINKGDVVNAGIN 189
           AGPGGYLQYLVYSITSLGFVQQSFDAIVNAIEQSIIQAH+SLKPG I  NKG+V NAGIN
Sbjct: 121 AGPGGYLQYLVYSITSLGFVQQSFDAIVNAIEQSIIQAHESLKPGFILFNKGEVDNAGIN 180

Query: 190 RSPSAYLLNPQEERARYSTNVDTEMTVLKFVDGASGKSVGAFSWFPTHGTSMSRDNKLIS 249
           RSPSAYL+NP+EERA+Y+ NVD EM++LKFVDG SG S+GAFSWFPTHGTSMSRDNKLIS
Sbjct: 181 RSPSAYLMNPEEERAKYANNVDKEMSILKFVDGESGNSIGAFSWFPTHGTSMSRDNKLIS 240

Query: 250 GDNKGAAARFFEDWIASKRTKRSSNNNASDFNEMGREIFLSCQDFNEMGREILLKLLSVG 309
           GDNKGAAARFFEDW AS                                           
Sbjct: 241 GDNKGAAARFFEDWAAS------------------------------------------- 300

Query: 310 VGEKSASELGRNGGIGGSELIGVRSEGERNDGIGGSELVGVRSGRETTASAATALVDIVE 369
                                                     +GR TT  +  A V I E
Sbjct: 301 ------------------------------------------NGRTTTTPSNNASV-IEE 360

Query: 370 LMKKAETIKATGGKRCSKASSQASKVRNNDGSLFVAAFCQSNVGDVTPNVLGAFCTDSGN 429
           LMKKAE IKATGGK+CSK SS++SKVR NDGSLFVAAFCQSNVGDVTPNVLGAFC DSG 
Sbjct: 361 LMKKAEKIKATGGKQCSKTSSKSSKVRKNDGSLFVAAFCQSNVGDVTPNVLGAFCIDSGK 420

Query: 430 PCDFNRSSCHGNDLLCVGRGPGFPDEILSTKIIGERQFLKAADLFTTATEKLTGEIDFRH 489
           PCDFNRSSCHG+DLLCVGRGPGFPDEILSTKIIGERQFLKAADLFTTATEKLTGEIDFRH
Sbjct: 421 PCDFNRSSCHGSDLLCVGRGPGFPDEILSTKIIGERQFLKAADLFTTATEKLTGEIDFRH 480

Query: 490 VYLNFTDIEVELDGDNVVRTCPAALGPGFAAGTTDGPGVFGFQQGDTKINELWRKLRDSL 549
           VYLNFTDIEVE+DG+NVVRTCPAALGPGFAAGTTDGPGVFGFQQGDT+IN+LW++LRDSL
Sbjct: 481 VYLNFTDIEVEIDGNNVVRTCPAALGPGFAAGTTDGPGVFGFQQGDTEINKLWKQLRDSL 540

Query: 550 RKPSEFQVGCQKPKTVLLDTGEMFEPYAWAPAILPIQILRLGKLIILSVPGEFTTMAGRR 609
           +KPSEFQVGCQKPKTVLLDTGEMFEPYAWAPAILPIQILRLGKLIILSVPGEFTTMAGRR
Sbjct: 541 KKPSEFQVGCQKPKTVLLDTGEMFEPYAWAPAILPIQILRLGKLIILSVPGEFTTMAGRR 600

Query: 610 LREAVKETLISNGNGEFDDDTHVVIAGLTNTYSQYVATFEEYEQQRYEAASTLYGPHTLS 669
           LREAVKETLISNGNGEFDDDTHVVIAGLTNTYSQYVATFEEYEQQRYEAASTLYGPHTLS
Sbjct: 601 LREAVKETLISNGNGEFDDDTHVVIAGLTNTYSQYVATFEEYEQQRYEAASTLYGPHTLS 660

Query: 670 AYIQEFKKLATAIAKGENIVKPIASPPDLSSVQLRFVLEPFGESPPNGINFGDIQQDIKL 729
           AYIQEFKKLA+AIAKGE + KPIASPPDLSSVQLR V++PFGESPP+G+NFGDIQQD++L
Sbjct: 661 AYIQEFKKLASAIAKGEKVAKPIASPPDLSSVQLRLVMDPFGESPPDGVNFGDIQQDVEL 720

Query: 730 PKTGWFKRGDKQKPTATFWTANPRFDLLTEGTYAVVERLEKQRWKPAYDDDDFSLLFKWT 789
           PK GWFK G KQKPTATFW+ANPRFDLLTEGTYA+VERLEKQRW PAYDDDDFS+ FKW 
Sbjct: 721 PKDGWFKTGSKQKPTATFWSANPRFDLLTEGTYALVERLEKQRWTPAYDDDDFSVSFKWK 747

Query: 790 LDNNSFINSLATIEWDIPTDADPGVYRLRHFGSSKTTTNSSNIYFSGASRAFAV 835
           LDN + INSLATIEWDIP DADPGVYRLRHFGSS++T NS+NIYF+GASRAFAV
Sbjct: 781 LDNTTLINSLATIEWDIPIDADPGVYRLRHFGSSRSTINSTNIYFTGASRAFAV 747

BLAST of Lag0004087 vs. ExPASy TrEMBL
Match: A0A6J5W870 (Neutral ceramidase OS=Prunus armeniaca OX=36596 GN=CURHAP_LOCUS7600 PE=3 SV=1)

HSP 1 Score: 1144.8 bits (2960), Expect = 0.0e+00
Identity = 585/815 (71.78%), Postives = 652/815 (80.00%), Query Frame = 0

Query: 21  CVNGDWLVGVGSFDMTGPAAQVNMMGYANMDQNTAGVHFRLRARTFVVAKTVDGPRFAFV 80
           C    +L+GVGS+DMTGPAA VNMMGYANMDQ+TAGVHFRLRARTF+VA++  GPRFAFV
Sbjct: 32  CNGEGYLIGVGSYDMTGPAAGVNMMGYANMDQSTAGVHFRLRARTFIVAESSQGPRFAFV 91

Query: 81  NLDAGMASQLVTIKVLERLKSRFGDLYTEENVAISGIHTHAGPGGYLQYLVYSITSLGFV 140
           NLDAGMASQLV IKVL++LKSRFGDLYTEENVAISGIHTHAGPGGYLQYLVYSITSLGFV
Sbjct: 92  NLDAGMASQLVNIKVLDKLKSRFGDLYTEENVAISGIHTHAGPGGYLQYLVYSITSLGFV 151

Query: 141 QQSFDAIVNAIEQSIIQAHQSLKPGSIFINKGDVVNAGINRSPSAYLLNPQEERARYSTN 200
           QQSFDAIVNAIEQSI+QAH +LKPGS+FINKGDVVNAGINRSPSAYLLNP EERARY +N
Sbjct: 152 QQSFDAIVNAIEQSIVQAHHNLKPGSVFINKGDVVNAGINRSPSAYLLNPAEERARYPSN 211

Query: 201 VDTEMTVLKFVDGASGKSVGAFSWFPTHGTSMSRDNKLISGDNKGAAARFFEDWIASKRT 260
           VDT MT+LKFVD ASGKSVGAFSWF THGTSMS++N LISGDNKGAAARFFEDW +S   
Sbjct: 212 VDTLMTLLKFVDDASGKSVGAFSWFATHGTSMSKNNLLISGDNKGAAARFFEDWFSS--- 271

Query: 261 KRSSNNNASDFNEMGREIFLSCQDFNEMGREILLKLLSVGVGEKSASELGRNGGIGGSEL 320
             SS+  +SD                                                  
Sbjct: 272 SSSSSTTSSD-------------------------------------------------- 331

Query: 321 IGVRSEGERNDGIGGSELVGVRSGRETTASAATALVDIVELMKKAETIKATGGKRCSKAS 380
                                     ++ + A +   ++ L+KKA+++KATGGK C K S
Sbjct: 332 -------------------------SSSTTPADSSASLLSLIKKAQSMKATGGKLCGKRS 391

Query: 381 SQASKVRNNDGSLFVAAFCQSNVGDVTPNVLGAFCTDSGNPCDFNRSSCHGNDLLCVGRG 440
           S+ SKVR N+GSLFV AFCQSNVGDVTPNVLGAFCTDSG PCDFN SSCHGNDLLC+GRG
Sbjct: 392 SRESKVRKNNGSLFVGAFCQSNVGDVTPNVLGAFCTDSGRPCDFNHSSCHGNDLLCLGRG 451

Query: 441 PGFPDEILSTKIIGERQFLKAADLFTTATEKLTGEIDFRHVYLNFTDIEVE-LDGDNVVR 500
           PG+PDEILSTKIIGERQF KAA LFTTAT++LTG ID+RHVYLNFTDIEVE LD +  V+
Sbjct: 452 PGYPDEILSTKIIGERQFQKAAHLFTTATQQLTGTIDYRHVYLNFTDIEVELLDRNEKVK 511

Query: 501 TCPAALGPGFAAGTTDGPGVFGFQQGDTKINELWRKLRDSLRKPSEFQVGCQKPKTVLLD 560
           TCPAALGPGFAAGTTDGPG FGFQQGDTKINELWRKLRD+L+KP+ +QV CQKPKTVLLD
Sbjct: 512 TCPAALGPGFAAGTTDGPGAFGFQQGDTKINELWRKLRDALKKPTPYQVDCQKPKTVLLD 571

Query: 561 TGEMFEPYAWAPAILPIQILRLGKLIILSVPGEFTTMAGRRLREAVKETLISNGNGEFDD 620
           TGEMF PYAWAPAILPIQ+LRLGKLIILSVPGEFTTMAGRRLREAVKETLISN NGEFDD
Sbjct: 572 TGEMFVPYAWAPAILPIQMLRLGKLIILSVPGEFTTMAGRRLREAVKETLISNSNGEFDD 631

Query: 621 DTHVVIAGLTNTYSQYVATFEEYEQQRYEAASTLYGPHTLSAYIQEFKKLATAIAKGENI 680
           +THVVIAGLTNTYSQY+ATFEEY QQRYEAASTLYGPHTLSAYIQEF+KLA A+AKGE I
Sbjct: 632 ETHVVIAGLTNTYSQYIATFEEYAQQRYEAASTLYGPHTLSAYIQEFEKLAKAMAKGEKI 691

Query: 681 VKPIASPPDLSSVQLRFVLEPFGESPPNGINFGDIQQDIKLPKTGWFKRGDKQKPTATFW 740
            K   SPPDLSSVQL+ +LEP G+ PP   NFGD++QDI LPK+G FK+GD  +P ATFW
Sbjct: 692 TKG-PSPPDLSSVQLKLLLEPTGDLPPPHTNFGDMEQDIILPKSGSFKKGD--RPIATFW 751

Query: 741 TANPRFDLLTEGTYAVVERLEKQRWKPAYDDDDFSLLFKWTLDNNSFINSLATIEWDIPT 800
           +ANPR+DLLTEGT+AVVE L+ + W P YDDDDF L FKW +DN++   + ATIEW+IP 
Sbjct: 752 SANPRYDLLTEGTFAVVELLQGKHWVPVYDDDDFCLFFKWKVDNSTLYGT-ATIEWEIPR 764

Query: 801 DADPGVYRLRHFGSSKTTTNSSNIYFSGASRAFAV 835
           DAD GVYRLRHFGSSK T +S NIYF+GAS  FAV
Sbjct: 812 DADSGVYRLRHFGSSKKTKDSPNIYFTGASSGFAV 764

BLAST of Lag0004087 vs. ExPASy TrEMBL
Match: A0A251R941 (Neutral ceramidase OS=Prunus persica OX=3760 GN=PRUPE_1G356500 PE=3 SV=1)

HSP 1 Score: 1138.6 bits (2944), Expect = 0.0e+00
Identity = 581/815 (71.29%), Postives = 649/815 (79.63%), Query Frame = 0

Query: 21  CVNGDWLVGVGSFDMTGPAAQVNMMGYANMDQNTAGVHFRLRARTFVVAKTVDGPRFAFV 80
           C    +L+GVGS+DMTGPAA+VNMMGYANMDQ+TAGVHFRLRARTF+VA++  GPRFAFV
Sbjct: 32  CNGEGYLIGVGSYDMTGPAAEVNMMGYANMDQSTAGVHFRLRARTFIVAESSQGPRFAFV 91

Query: 81  NLDAGMASQLVTIKVLERLKSRFGDLYTEENVAISGIHTHAGPGGYLQYLVYSITSLGFV 140
           NLDAGMASQLV IKVL++LKSRFGDLYTEENVAISGIHTHAGPGGYLQYLVYSITSLGFV
Sbjct: 92  NLDAGMASQLVNIKVLDKLKSRFGDLYTEENVAISGIHTHAGPGGYLQYLVYSITSLGFV 151

Query: 141 QQSFDAIVNAIEQSIIQAHQSLKPGSIFINKGDVVNAGINRSPSAYLLNPQEERARYSTN 200
           QQSFDAIVNAIEQSI+QAH +LKPGS+FIN+GDVVNAGINRSPSAYLLNP EERARY +N
Sbjct: 152 QQSFDAIVNAIEQSIVQAHHNLKPGSVFINEGDVVNAGINRSPSAYLLNPAEERARYPSN 211

Query: 201 VDTEMTVLKFVDGASGKSVGAFSWFPTHGTSMSRDNKLISGDNKGAAARFFEDWIASKRT 260
           VDT MT+LKFVD ASGKSVGAFSWF THGTSMS++N LISGDNKGAAARFFEDW +S   
Sbjct: 212 VDTLMTLLKFVDDASGKSVGAFSWFATHGTSMSKNNLLISGDNKGAAARFFEDWFSSS-- 271

Query: 261 KRSSNNNASDFNEMGREIFLSCQDFNEMGREILLKLLSVGVGEKSASELGRNGGIGGSEL 320
                                                                       
Sbjct: 272 ------------------------------------------------------------ 331

Query: 321 IGVRSEGERNDGIGGSELVGVRSGRETTASAATALVDIVELMKKAETIKATGGKRCSKAS 380
                                 S   TT++ ++A   ++ L+KKA+++KATGGK C K S
Sbjct: 332 ----------------------SSSSTTSADSSA--SLLSLIKKAQSMKATGGKLCGKTS 391

Query: 381 SQASKVRNNDGSLFVAAFCQSNVGDVTPNVLGAFCTDSGNPCDFNRSSCHGNDLLCVGRG 440
           S+ SKVR N+GSLFV AFCQSNVGDVTPNVLGAFC DSG PCDFN SSCHGNDLLC+GRG
Sbjct: 392 SRESKVRKNNGSLFVGAFCQSNVGDVTPNVLGAFCADSGRPCDFNHSSCHGNDLLCLGRG 451

Query: 441 PGFPDEILSTKIIGERQFLKAADLFTTATEKLTGEIDFRHVYLNFTDIEVE-LDGDNVVR 500
           PG+PDEILSTKIIGERQF KAA LFTTAT++LTG ID+RHVYLNFTDIEVE LDG+  V+
Sbjct: 452 PGYPDEILSTKIIGERQFQKAAHLFTTATQQLTGTIDYRHVYLNFTDIEVELLDGNKKVK 511

Query: 501 TCPAALGPGFAAGTTDGPGVFGFQQGDTKINELWRKLRDSLRKPSEFQVGCQKPKTVLLD 560
           TCPAALGPGFAAGTTDGPG FGFQQGDTKIN+LWRKLRD+L+KP+ +QV CQKPKTVLLD
Sbjct: 512 TCPAALGPGFAAGTTDGPGAFGFQQGDTKINKLWRKLRDALKKPTPYQVDCQKPKTVLLD 571

Query: 561 TGEMFEPYAWAPAILPIQILRLGKLIILSVPGEFTTMAGRRLREAVKETLISNGNGEFDD 620
           TGEMF PYAWAPAILPIQ+LRLGKLIILSVPGEFTTMAGRRLREAVKETLISN NGEFDD
Sbjct: 572 TGEMFVPYAWAPAILPIQMLRLGKLIILSVPGEFTTMAGRRLREAVKETLISNSNGEFDD 631

Query: 621 DTHVVIAGLTNTYSQYVATFEEYEQQRYEAASTLYGPHTLSAYIQEFKKLATAIAKGENI 680
           +TH+VIAGLTNTYSQY+ATFEEY QQRYEAASTLYGPHTLSAYIQEF+KLA A+AKGE I
Sbjct: 632 ETHIVIAGLTNTYSQYIATFEEYAQQRYEAASTLYGPHTLSAYIQEFEKLAKAMAKGEQI 691

Query: 681 VKPIASPPDLSSVQLRFVLEPFGESPPNGINFGDIQQDIKLPKTGWFKRGDKQKPTATFW 740
            K   SPPDLSSVQLR +LEP G+ PP   NFGD++QDI +PK+G FK+GD  +P ATFW
Sbjct: 692 TKG-PSPPDLSSVQLRLLLEPTGDLPPPHTNFGDMKQDIIIPKSGSFKKGD--RPIATFW 751

Query: 741 TANPRFDLLTEGTYAVVERLEKQRWKPAYDDDDFSLLFKWTLDNNSFINSLATIEWDIPT 800
           +ANPR+DLLTEGT+AVVE L+ + W P YDDDDF L FKW +DN +   + ATIEW+IP 
Sbjct: 752 SANPRYDLLTEGTFAVVELLQGKHWVPVYDDDDFCLFFKWKVDNITLYGT-ATIEWEIPR 756

Query: 801 DADPGVYRLRHFGSSKTTTNSSNIYFSGASRAFAV 835
           DAD GVYRLRHFGSSK T +S NIYF+GAS  FAV
Sbjct: 812 DADSGVYRLRHFGSSKKTKDSPNIYFTGASSGFAV 756

BLAST of Lag0004087 vs. ExPASy TrEMBL
Match: A0A5E4FCT8 (Neutral ceramidase OS=Prunus dulcis OX=3755 GN=ALMOND_2B003982 PE=3 SV=1)

HSP 1 Score: 1135.6 bits (2936), Expect = 0.0e+00
Identity = 581/815 (71.29%), Postives = 648/815 (79.51%), Query Frame = 0

Query: 21  CVNGDWLVGVGSFDMTGPAAQVNMMGYANMDQNTAGVHFRLRARTFVVAKTVDGPRFAFV 80
           C    +L+GVGS+DMTGPAA VNMMGYANMDQ+TAGVHFRLRARTF+VA++  GPRFAFV
Sbjct: 19  CNGEGYLIGVGSYDMTGPAAGVNMMGYANMDQSTAGVHFRLRARTFIVAESSQGPRFAFV 78

Query: 81  NLDAGMASQLVTIKVLERLKSRFGDLYTEENVAISGIHTHAGPGGYLQYLVYSITSLGFV 140
           NLDAGMASQLV IKVL++LKSRFGDLYTEENVAISGIHTHAGPGGYLQYLVYSITSLGFV
Sbjct: 79  NLDAGMASQLVNIKVLDKLKSRFGDLYTEENVAISGIHTHAGPGGYLQYLVYSITSLGFV 138

Query: 141 QQSFDAIVNAIEQSIIQAHQSLKPGSIFINKGDVVNAGINRSPSAYLLNPQEERARYSTN 200
           QQSFDAIVNAIEQSI+QAH +LKP S+FIN+GDVVNAGINRSPSAYLLNP EERARY +N
Sbjct: 139 QQSFDAIVNAIEQSIVQAHHNLKPASVFINEGDVVNAGINRSPSAYLLNPAEERARYPSN 198

Query: 201 VDTEMTVLKFVDGASGKSVGAFSWFPTHGTSMSRDNKLISGDNKGAAARFFEDWIASKRT 260
           VDT MT+LKFVD ASGKSVGAFSWF THGTSMS++N LISGDNKGAAARFFEDW +S   
Sbjct: 199 VDTLMTLLKFVDDASGKSVGAFSWFATHGTSMSKNNLLISGDNKGAAARFFEDWFSSS-- 258

Query: 261 KRSSNNNASDFNEMGREIFLSCQDFNEMGREILLKLLSVGVGEKSASELGRNGGIGGSEL 320
                                                                       
Sbjct: 259 ------------------------------------------------------------ 318

Query: 321 IGVRSEGERNDGIGGSELVGVRSGRETTASAATALVDIVELMKKAETIKATGGKRCSKAS 380
                                 S   TT++ ++A   ++ L+KKA+++KATGGK C K S
Sbjct: 319 ----------------------SSSSTTSADSSA--SLLSLIKKAQSMKATGGKLCGKTS 378

Query: 381 SQASKVRNNDGSLFVAAFCQSNVGDVTPNVLGAFCTDSGNPCDFNRSSCHGNDLLCVGRG 440
           S+ SKVR N+GSLFV AFCQSNVGDVTPNVLGAFCTDSG PCDFN SSCHGNDLLC+GRG
Sbjct: 379 SRESKVRKNNGSLFVGAFCQSNVGDVTPNVLGAFCTDSGRPCDFNHSSCHGNDLLCLGRG 438

Query: 441 PGFPDEILSTKIIGERQFLKAADLFTTATEKLTGEIDFRHVYLNFTDIEVE-LDGDNVVR 500
           PG+PDEILSTKIIGERQF KAA LFTTAT++LTG ID+RHVYLNFTDIEVE LDG+  V+
Sbjct: 439 PGYPDEILSTKIIGERQFQKAAHLFTTATQQLTGTIDYRHVYLNFTDIEVELLDGNKKVK 498

Query: 501 TCPAALGPGFAAGTTDGPGVFGFQQGDTKINELWRKLRDSLRKPSEFQVGCQKPKTVLLD 560
           TCPAALGPGFAAGTTDGPG FGFQQGDTKIN+LWRKLRD+L+KP+ +QV CQKPKTVLLD
Sbjct: 499 TCPAALGPGFAAGTTDGPGAFGFQQGDTKINKLWRKLRDALKKPTPYQVDCQKPKTVLLD 558

Query: 561 TGEMFEPYAWAPAILPIQILRLGKLIILSVPGEFTTMAGRRLREAVKETLISNGNGEFDD 620
           TGEMF PYAWAPAILPIQ+LRLGKLIILSVPGEFTTMAGRRLREAVKETLISN NGEFDD
Sbjct: 559 TGEMFVPYAWAPAILPIQMLRLGKLIILSVPGEFTTMAGRRLREAVKETLISNSNGEFDD 618

Query: 621 DTHVVIAGLTNTYSQYVATFEEYEQQRYEAASTLYGPHTLSAYIQEFKKLATAIAKGENI 680
           +TH+VIAGLTNTYSQY+ATFEEY QQRYEAASTLYGPHTLSAYIQEF+KLA A+AKGE I
Sbjct: 619 ETHIVIAGLTNTYSQYIATFEEYAQQRYEAASTLYGPHTLSAYIQEFEKLAKAMAKGEKI 678

Query: 681 VKPIASPPDLSSVQLRFVLEPFGESPPNGINFGDIQQDIKLPKTGWFKRGDKQKPTATFW 740
            K   SPPDLSSVQLR +LEP G+ PP   NFGD++QDI LPK+G FK+GD  +P ATFW
Sbjct: 679 TKG-PSPPDLSSVQLRLLLEPTGDLPPPHTNFGDMKQDIILPKSGSFKKGD--RPIATFW 738

Query: 741 TANPRFDLLTEGTYAVVERLEKQRWKPAYDDDDFSLLFKWTLDNNSFINSLATIEWDIPT 800
           +ANPR+DLLTEGT+AVVE L+ + W P YDDDDF L FKW +DN++   + ATIEW+IP 
Sbjct: 739 SANPRYDLLTEGTFAVVELLQGKHWVPVYDDDDFCLFFKWKVDNSTLYGT-ATIEWEIPR 743

Query: 801 DADPGVYRLRHFGSSKTTTNSSNIYFSGASRAFAV 835
           DA  GVYRLRHFGSSK T +S NIYF+GAS  FAV
Sbjct: 799 DAVSGVYRLRHFGSSKKTKDSPNIYFTGASSGFAV 743

BLAST of Lag0004087 vs. ExPASy TrEMBL
Match: A0A5E4FD18 (Neutral ceramidase OS=Prunus dulcis OX=3755 GN=ALMOND_2B003982 PE=3 SV=1)

HSP 1 Score: 1135.6 bits (2936), Expect = 0.0e+00
Identity = 581/815 (71.29%), Postives = 648/815 (79.51%), Query Frame = 0

Query: 21  CVNGDWLVGVGSFDMTGPAAQVNMMGYANMDQNTAGVHFRLRARTFVVAKTVDGPRFAFV 80
           C    +L+GVGS+DMTGPAA VNMMGYANMDQ+TAGVHFRLRARTF+VA++  GPRFAFV
Sbjct: 32  CNGEGYLIGVGSYDMTGPAAGVNMMGYANMDQSTAGVHFRLRARTFIVAESSQGPRFAFV 91

Query: 81  NLDAGMASQLVTIKVLERLKSRFGDLYTEENVAISGIHTHAGPGGYLQYLVYSITSLGFV 140
           NLDAGMASQLV IKVL++LKSRFGDLYTEENVAISGIHTHAGPGGYLQYLVYSITSLGFV
Sbjct: 92  NLDAGMASQLVNIKVLDKLKSRFGDLYTEENVAISGIHTHAGPGGYLQYLVYSITSLGFV 151

Query: 141 QQSFDAIVNAIEQSIIQAHQSLKPGSIFINKGDVVNAGINRSPSAYLLNPQEERARYSTN 200
           QQSFDAIVNAIEQSI+QAH +LKP S+FIN+GDVVNAGINRSPSAYLLNP EERARY +N
Sbjct: 152 QQSFDAIVNAIEQSIVQAHHNLKPASVFINEGDVVNAGINRSPSAYLLNPAEERARYPSN 211

Query: 201 VDTEMTVLKFVDGASGKSVGAFSWFPTHGTSMSRDNKLISGDNKGAAARFFEDWIASKRT 260
           VDT MT+LKFVD ASGKSVGAFSWF THGTSMS++N LISGDNKGAAARFFEDW +S   
Sbjct: 212 VDTLMTLLKFVDDASGKSVGAFSWFATHGTSMSKNNLLISGDNKGAAARFFEDWFSSS-- 271

Query: 261 KRSSNNNASDFNEMGREIFLSCQDFNEMGREILLKLLSVGVGEKSASELGRNGGIGGSEL 320
                                                                       
Sbjct: 272 ------------------------------------------------------------ 331

Query: 321 IGVRSEGERNDGIGGSELVGVRSGRETTASAATALVDIVELMKKAETIKATGGKRCSKAS 380
                                 S   TT++ ++A   ++ L+KKA+++KATGGK C K S
Sbjct: 332 ----------------------SSSSTTSADSSA--SLLSLIKKAQSMKATGGKLCGKTS 391

Query: 381 SQASKVRNNDGSLFVAAFCQSNVGDVTPNVLGAFCTDSGNPCDFNRSSCHGNDLLCVGRG 440
           S+ SKVR N+GSLFV AFCQSNVGDVTPNVLGAFCTDSG PCDFN SSCHGNDLLC+GRG
Sbjct: 392 SRESKVRKNNGSLFVGAFCQSNVGDVTPNVLGAFCTDSGRPCDFNHSSCHGNDLLCLGRG 451

Query: 441 PGFPDEILSTKIIGERQFLKAADLFTTATEKLTGEIDFRHVYLNFTDIEVE-LDGDNVVR 500
           PG+PDEILSTKIIGERQF KAA LFTTAT++LTG ID+RHVYLNFTDIEVE LDG+  V+
Sbjct: 452 PGYPDEILSTKIIGERQFQKAAHLFTTATQQLTGTIDYRHVYLNFTDIEVELLDGNKKVK 511

Query: 501 TCPAALGPGFAAGTTDGPGVFGFQQGDTKINELWRKLRDSLRKPSEFQVGCQKPKTVLLD 560
           TCPAALGPGFAAGTTDGPG FGFQQGDTKIN+LWRKLRD+L+KP+ +QV CQKPKTVLLD
Sbjct: 512 TCPAALGPGFAAGTTDGPGAFGFQQGDTKINKLWRKLRDALKKPTPYQVDCQKPKTVLLD 571

Query: 561 TGEMFEPYAWAPAILPIQILRLGKLIILSVPGEFTTMAGRRLREAVKETLISNGNGEFDD 620
           TGEMF PYAWAPAILPIQ+LRLGKLIILSVPGEFTTMAGRRLREAVKETLISN NGEFDD
Sbjct: 572 TGEMFVPYAWAPAILPIQMLRLGKLIILSVPGEFTTMAGRRLREAVKETLISNSNGEFDD 631

Query: 621 DTHVVIAGLTNTYSQYVATFEEYEQQRYEAASTLYGPHTLSAYIQEFKKLATAIAKGENI 680
           +TH+VIAGLTNTYSQY+ATFEEY QQRYEAASTLYGPHTLSAYIQEF+KLA A+AKGE I
Sbjct: 632 ETHIVIAGLTNTYSQYIATFEEYAQQRYEAASTLYGPHTLSAYIQEFEKLAKAMAKGEKI 691

Query: 681 VKPIASPPDLSSVQLRFVLEPFGESPPNGINFGDIQQDIKLPKTGWFKRGDKQKPTATFW 740
            K   SPPDLSSVQLR +LEP G+ PP   NFGD++QDI LPK+G FK+GD  +P ATFW
Sbjct: 692 TKG-PSPPDLSSVQLRLLLEPTGDLPPPHTNFGDMKQDIILPKSGSFKKGD--RPIATFW 751

Query: 741 TANPRFDLLTEGTYAVVERLEKQRWKPAYDDDDFSLLFKWTLDNNSFINSLATIEWDIPT 800
           +ANPR+DLLTEGT+AVVE L+ + W P YDDDDF L FKW +DN++   + ATIEW+IP 
Sbjct: 752 SANPRYDLLTEGTFAVVELLQGKHWVPVYDDDDFCLFFKWKVDNSTLYGT-ATIEWEIPR 756

Query: 801 DADPGVYRLRHFGSSKTTTNSSNIYFSGASRAFAV 835
           DA  GVYRLRHFGSSK T +S NIYF+GAS  FAV
Sbjct: 812 DAVSGVYRLRHFGSSKKTKDSPNIYFTGASSGFAV 756

BLAST of Lag0004087 vs. TAIR 10
Match: AT2G38010.2 (Neutral/alkaline non-lysosomal ceramidase )

HSP 1 Score: 842.0 bits (2174), Expect = 4.0e-244
Identity = 446/816 (54.66%), Postives = 559/816 (68.50%), Query Frame = 0

Query: 26  WLVGVGSFDMTGPAAQVNMMGYANMDQNTAGVHFRLRARTFVVAKTVDGPRFAFVNLDAG 85
           +L+GVGS+D+TGPAA VNMMGYAN DQ  +G+HFRLRAR F+VA+   G R  FVNLDA 
Sbjct: 26  YLIGVGSYDITGPAADVNMMGYANSDQIASGIHFRLRARAFIVAEP-QGNRVVFVNLDAC 85

Query: 86  MASQLVTIKVLERLKSRFGDLYTEENVAISGIHTHAGPGGYLQYLVYSITSLGFVQQSFD 145
           MASQ+VTIKVLERLK+R+G+LYTE+NVAISGIHTHAGPGGYLQY+ Y +TSLGFV+QSFD
Sbjct: 86  MASQIVTIKVLERLKARYGELYTEKNVAISGIHTHAGPGGYLQYVTYIVTSLGFVRQSFD 145

Query: 146 AIVNAIEQSIIQAHQSLKPGSIFINKGDVVNAGINRSPSAYLLNPQEERARYSTNVDTEM 205
            +VN IEQSI+QAH+SL+PGS F+NKGD+++AG+NRSPS+YL NP  ER++Y  +VD EM
Sbjct: 146 VVVNGIEQSIVQAHESLRPGSAFVNKGDLLDAGVNRSPSSYLNNPAAERSKYKYDVDKEM 205

Query: 206 TVLKFVDGASGKSVGAFSWFPTHGTSMSRDNKLISGDNKGAAARFFEDWIASKRTKRSSN 265
           T++KFVD   G + G+F+WF THGTSMSR N LISGDNKGAAARF EDW  + +    S+
Sbjct: 206 TLVKFVDSQLGPT-GSFNWFATHGTSMSRTNSLISGDNKGAAARFMEDWFENGQKNSVSS 265

Query: 266 NNASDFNEMGREIFLSCQDFNEMGREI-LLKLLSVGVGEKSASELGRNGGIGGSELIGVR 325
            N      + R +     DF+        L+L +V +                       
Sbjct: 266 RN------IPRRVSTIVSDFSRNRESYPFLELPNVAI----------------------- 325

Query: 326 SEGERNDGIGGSELVGVRSGRETTASAATALVDIVELMKKAETIKATGGKRCSKASSQAS 385
                         + V              V    L+  A T K++ G    K+    +
Sbjct: 326 ------------LYIAVAERLMWKVMVLFYFVSESRLLDIAATYKSSRGHSVDKSLDVKT 385

Query: 386 KVRNNDGSLFVAAFCQSNVGDVTPNVLGAFCTDSGNPCDFNRSSCHGNDLLCVGRGPGFP 445
           +VRN     FV+AFCQSN GDV+PN LG FC D+G PCDFN S+C+G + LC GRGPG+P
Sbjct: 386 RVRNGSKRKFVSAFCQSNCGDVSPNTLGTFCIDTGLPCDFNHSTCNGQNELCYGRGPGYP 445

Query: 446 DEILSTKIIGERQFLKAADLFTTATEKLTGEIDFRHVYLNFTDIEVEL----DGDNVVRT 505
           DE  ST+IIGE+QF  A +LF  ATEKL G+I ++H YL+F++++V +     G   V+T
Sbjct: 446 DEFESTRIIGEKQFKMAVELFNKATEKLQGKIGYQHAYLDFSNLDVTVPKAGGGSETVKT 505

Query: 506 CPAALGPGFAAGTTDGPGVFGFQQGDTKINELWRKLRDSLRKPSEFQVGCQKPKTVLLDT 565
           CPAA+G GFAAGTTDGPG F F+QGD + N  WR +R+ LR P   QV CQKPK +LLDT
Sbjct: 506 CPAAMGFGFAAGTTDGPGAFDFKQGDDQGNVFWRLVRNVLRTPGPEQVQCQKPKPILLDT 565

Query: 566 GEMFEPYAWAPAILPIQILRLGKLIILSVPGEFTTMAGRRLREAVKETLISNGNGEFDDD 625
           GEM EPY WAP+ILPIQILR+G+L+ILSVPGEFTTMAGRRLR+A+K  LIS+   EF ++
Sbjct: 566 GEMKEPYDWAPSILPIQILRIGQLVILSVPGEFTTMAGRRLRDAIKSFLISSDPKEFSNN 625

Query: 626 THVVIAGLTNTYSQYVATFEEYEQQRYEAASTLYGPHTLSAYIQEFKKLATAIAKGENIV 685
            HVVIAGLTNTYSQY+ATFEEYE QRYE ASTLYG HTL+AYIQEFKKLATA+  G  + 
Sbjct: 626 MHVVIAGLTNTYSQYIATFEEYEVQRYEGASTLYGRHTLTAYIQEFKKLATALVNGLTLP 685

Query: 686 KPIASPPDLSSVQLRFVLEPFGESPPNGINFGDIQQDIKLPKTGWFKRGDKQKPTATFWT 745
           +    PPDL   Q+  +     +S P G+ FGD++ D+  P    F+RG  Q+  ATFW+
Sbjct: 686 RG-PQPPDLLDKQISLLSPVVVDSTPLGVKFGDVKADV--PPKSTFRRG--QQVNATFWS 745

Query: 746 ANPRFDLLTEGTYAVVERL-EKQRWKPAYDDDDFSLLFKWTLDNNSFINSLATIEWDIPT 805
             PR DL+TEG++AVVE L E  +W P YDDDDFSL FKW+        S ATIEW +P 
Sbjct: 746 GCPRNDLMTEGSFAVVETLREGGKWAPVYDDDDFSLKFKWSRPAKLSSESQATIEWRVPE 792

Query: 806 DADPGVYRLRHFGSSKTTTNSSNIYFSGASRAFAVM 836
            A  GVYR+RH+G+SK+   S +  FSG+S AF V+
Sbjct: 806 SAVAGVYRIRHYGASKSLFGSIS-SFSGSSSAFVVV 792

BLAST of Lag0004087 vs. TAIR 10
Match: AT2G38010.1 (Neutral/alkaline non-lysosomal ceramidase )

HSP 1 Score: 837.0 bits (2161), Expect = 1.3e-242
Identity = 443/815 (54.36%), Postives = 553/815 (67.85%), Query Frame = 0

Query: 26  WLVGVGSFDMTGPAAQVNMMGYANMDQNTAGVHFRLRARTFVVAKTVDGPRFAFVNLDAG 85
           +L+GVGS+D+TGPAA VNMMGYAN DQ  +G+HFRLRAR F+VA+   G R  FVNLDA 
Sbjct: 26  YLIGVGSYDITGPAADVNMMGYANSDQIASGIHFRLRARAFIVAEP-QGNRVVFVNLDAC 85

Query: 86  MASQLVTIKVLERLKSRFGDLYTEENVAISGIHTHAGPGGYLQYLVYSITSLGFVQQSFD 145
           MASQ+VTIKVLERLK+R+G+LYTE+NVAISGIHTHAGPGGYLQY+ Y +TSLGFV+QSFD
Sbjct: 86  MASQIVTIKVLERLKARYGELYTEKNVAISGIHTHAGPGGYLQYVTYIVTSLGFVRQSFD 145

Query: 146 AIVNAIEQSIIQAHQSLKPGSIFINKGDVVNAGINRSPSAYLLNPQEERARYSTNVDTEM 205
            +VN IEQSI+QAH+SL+PGS F+NKGD+++AG+NRSPS+YL NP  ER++Y  +VD EM
Sbjct: 146 VVVNGIEQSIVQAHESLRPGSAFVNKGDLLDAGVNRSPSSYLNNPAAERSKYKYDVDKEM 205

Query: 206 TVLKFVDGASGKSVGAFSWFPTHGTSMSRDNKLISGDNKGAAARFFEDWIASKRTKRSSN 265
           T++KFVD   G + G+F+WF THGTSMSR N LISGDNKGAAARF EDW  + +    S+
Sbjct: 206 TLVKFVDSQLGPT-GSFNWFATHGTSMSRTNSLISGDNKGAAARFMEDWFENGQKNSVSS 265

Query: 266 NNASDFNEMGREIFLSCQDFNEMGREILLKLLSVGVGEKSASELGRNGGIGGSELIGVRS 325
            N      + R +     DF+   R+ LL +                             
Sbjct: 266 RN------IPRRVSTIVSDFSR-NRDRLLDI----------------------------- 325

Query: 326 EGERNDGIGGSELVGVRSGRETTASAATALVDIVELMKKAETIKATGGKRCSKASSQASK 385
                                                  A T K++ G    K+    ++
Sbjct: 326 ---------------------------------------AATYKSSRGHSVDKSLDVKTR 385

Query: 386 VRNNDGSLFVAAFCQSNVGDVTPNVLGAFCTDSGNPCDFNRSSCHGNDLLCVGRGPGFPD 445
           VRN     FV+AFCQSN GDV+PN LG FC D+G PCDFN S+C+G + LC GRGPG+PD
Sbjct: 386 VRNGSKRKFVSAFCQSNCGDVSPNTLGTFCIDTGLPCDFNHSTCNGQNELCYGRGPGYPD 445

Query: 446 EILSTKIIGERQFLKAADLFTTATEKLTGEIDFRHVYLNFTDIEVEL----DGDNVVRTC 505
           E  ST+IIGE+QF  A +LF  ATEKL G+I ++H YL+F++++V +     G   V+TC
Sbjct: 446 EFESTRIIGEKQFKMAVELFNKATEKLQGKIGYQHAYLDFSNLDVTVPKAGGGSETVKTC 505

Query: 506 PAALGPGFAAGTTDGPGVFGFQQGDTKINELWRKLRDSLRKPSEFQVGCQKPKTVLLDTG 565
           PAA+G GFAAGTTDGPG F F+QGD + N  WR +R+ LR P   QV CQKPK +LLDTG
Sbjct: 506 PAAMGFGFAAGTTDGPGAFDFKQGDDQGNVFWRLVRNVLRTPGPEQVQCQKPKPILLDTG 565

Query: 566 EMFEPYAWAPAILPIQILRLGKLIILSVPGEFTTMAGRRLREAVKETLISNGNGEFDDDT 625
           EM EPY WAP+ILPIQILR+G+L+ILSVPGEFTTMAGRRLR+A+K  LIS+   EF ++ 
Sbjct: 566 EMKEPYDWAPSILPIQILRIGQLVILSVPGEFTTMAGRRLRDAIKSFLISSDPKEFSNNM 625

Query: 626 HVVIAGLTNTYSQYVATFEEYEQQRYEAASTLYGPHTLSAYIQEFKKLATAIAKGENIVK 685
           HVVIAGLTNTYSQY+ATFEEYE QRYE ASTLYG HTL+AYIQEFKKLATA+  G  + +
Sbjct: 626 HVVIAGLTNTYSQYIATFEEYEVQRYEGASTLYGRHTLTAYIQEFKKLATALVNGLTLPR 685

Query: 686 PIASPPDLSSVQLRFVLEPFGESPPNGINFGDIQQDIKLPKTGWFKRGDKQKPTATFWTA 745
               PPDL   Q+  +     +S P G+ FGD++ D+  P    F+RG  Q+  ATFW+ 
Sbjct: 686 G-PQPPDLLDKQISLLSPVVVDSTPLGVKFGDVKADV--PPKSTFRRG--QQVNATFWSG 745

Query: 746 NPRFDLLTEGTYAVVERL-EKQRWKPAYDDDDFSLLFKWTLDNNSFINSLATIEWDIPTD 805
            PR DL+TEG++AVVE L E  +W P YDDDDFSL FKW+        S ATIEW +P  
Sbjct: 746 CPRNDLMTEGSFAVVETLREGGKWAPVYDDDDFSLKFKWSRPAKLSSESQATIEWRVPES 757

Query: 806 ADPGVYRLRHFGSSKTTTNSSNIYFSGASRAFAVM 836
           A  GVYR+RH+G+SK+   S +  FSG+S AF V+
Sbjct: 806 AVAGVYRIRHYGASKSLFGSIS-SFSGSSSAFVVV 757

BLAST of Lag0004087 vs. TAIR 10
Match: AT1G07380.1 (Neutral/alkaline non-lysosomal ceramidase )

HSP 1 Score: 806.6 bits (2082), Expect = 1.9e-233
Identity = 434/822 (52.80%), Postives = 555/822 (67.52%), Query Frame = 0

Query: 23  NGDWLVGVGSFDMTGPAAQVNMMGYANMDQNTAGVHFRLRARTFVVAKTVDGPRFAFVNL 82
           + ++L+G+GS+D+TGPAA VNMMGYANM+Q  +G+HFRLRARTF+V++   G R  FVNL
Sbjct: 34  HSEYLIGLGSYDITGPAADVNMMGYANMEQVASGIHFRLRARTFIVSEP-QGKRVVFVNL 93

Query: 83  DAGMASQLVTIKVLERLKSRFGDLYTEENVAISGIHTHAGPGGYLQYLVYSITSLGFVQQ 142
           DA MASQ+V +KV+ERLK+R+GDLYTE+NV ISGIHTHAGPGGYLQY+VY +TSLGFV+Q
Sbjct: 94  DACMASQIVKLKVIERLKARYGDLYTEQNVGISGIHTHAGPGGYLQYVVYIVTSLGFVRQ 153

Query: 143 SFDAIVNAIEQSIIQAHQSLKPGSIFINKGDVVNAGINRSPSAYLLNPQEERARYSTNVD 202
           SFDA+V+ IE SIIQAH++L+PGSIF+N G++++AG+NRSPSAYL NP +ER+++  NVD
Sbjct: 154 SFDALVDGIENSIIQAHENLRPGSIFLNNGELLDAGVNRSPSAYLNNPSKERSKHKYNVD 213

Query: 203 TEMTVLKFVDGASGKSVGAFSWFPTHGTSMSRDNKLISGDNKGAAARFFEDWIASKRTKR 262
            EMT+LKFVD   G  VG+F+WF THGTSMSR N LISGDNKGAA+RF EDW      +R
Sbjct: 214 KEMTLLKFVDDQWG-PVGSFNWFATHGTSMSRTNSLISGDNKGAASRFMEDWYEQNTAER 273

Query: 263 SSNNNASDFNEMGREIFLSCQDFNEMGREILLKLLSVGVGEKSASELGRNGGIGGSELIG 322
           S +           E F+S    +E+ R +                         S LI 
Sbjct: 274 SYS-----------EEFIS----DEIPRRV-------------------------SSLIE 333

Query: 323 VRSEGERNDGIGGSELVGVRSGRETTASAATALVDIVELMKKAETIKATGGKRCSKASSQ 382
              +                                 EL++ A   ++  GK  ++ SS 
Sbjct: 334 NHQDSHH------------------------------ELLELASYFESQPGKPVTRISSS 393

Query: 383 ASKVRN----NDGSLFVAAFCQSNVGDVTPNVLGAFCTDSGNPCDFNRSSCHGNDLLCVG 442
           A +VR+     D   FV+AFCQ+N GDV+PNVLGAFC D+G PCDFN S+C G + +C G
Sbjct: 394 ARRVRSALRKADKPGFVSAFCQTNCGDVSPNVLGAFCLDTGLPCDFNHSTCGGKNEMCYG 453

Query: 443 RGPGFPDEILSTKIIGERQFLKAADLFTTATEKLTGEIDFRHVYLNFTDIEVEL---DG- 502
           RGPG+PDE  ST+IIGERQF  A +LF  A+E+L G++D+RHVY++F+ + V L   DG 
Sbjct: 454 RGPGYPDEFESTRIIGERQFKMALELFNKASEQLQGKVDYRHVYVDFSQLNVTLPKKDGK 513

Query: 503 DNVVRTCPAALGPGFAAGTTDGPGVFGFQQGDTKINELWRKLRDSLRKPSEFQVGCQKPK 562
             VV+TCPAA+G  FAAGTTDGPG F F QGD K N  WR +R+ L+ P + Q+ C  PK
Sbjct: 514 SEVVKTCPAAMGFAFAAGTTDGPGAFDFTQGDDKGNPFWRLVRNVLKTPDKKQIDCHYPK 573

Query: 563 TVLLDTGEMFEPYAWAPAILPIQILRLGKLIILSVPGEFTTMAGRRLREAVKETLISNGN 622
            +LLDTGEM +PY WAP+IL +Q+LR+G+L ILSVPGEFTTMAGRRLR AVK  L ++GN
Sbjct: 574 PILLDTGEMTKPYDWAPSILSLQVLRIGQLFILSVPGEFTTMAGRRLRYAVKTQLKNSGN 633

Query: 623 GEFDDDTHVVIAGLTNTYSQYVATFEEYEQQRYEAASTLYGPHTLSAYIQEFKKLATAIA 682
            +   + HVVIAGL N YSQYV TFEEY+ QRYE ASTLYGPHTLS YIQEFKKL+ ++ 
Sbjct: 634 KDLSGEIHVVIAGLANGYSQYVTTFEEYQVQRYEGASTLYGPHTLSGYIQEFKKLSKSLV 693

Query: 683 KGENIVKPIASPPDLSSVQLRFVLEPFGESPPNGINFGDIQQDIKLPKTGWFKRGDKQKP 742
             +  V+P   PPDL   QL F+     ++ P+G +FGD+  D+  PK    KRG+ Q  
Sbjct: 694 L-DMPVQPGPQPPDLLDKQLSFLTPVMMDTTPSGDSFGDVISDV--PKNLSLKRGNGQ-V 753

Query: 743 TATFWTANPRFDLLTEGTYAVVERLEK--QRWKPAYDDDDFSLLFKWTLDNNSFINSLAT 802
           T  F +A PR DLLTEGT+ +VERLE+  + W P YDDDD  L FKW+        S AT
Sbjct: 754 TVVFRSACPRNDLLTEGTFTLVERLEQKDKTWTPVYDDDDLCLRFKWSRHKKLSSRSQAT 778

Query: 803 IEWDIPTDADPGVYRLRHFGSSKTTTNSSNIYFSGASRAFAV 835
           +EW IP  A PGVYR+ HFG++K    S + +F+G+S AF V
Sbjct: 814 VEWRIPESASPGVYRITHFGAAKKLFGSVH-HFTGSSSAFVV 778

BLAST of Lag0004087 vs. TAIR 10
Match: AT5G58980.1 (Neutral/alkaline non-lysosomal ceramidase )

HSP 1 Score: 765.0 bits (1974), Expect = 6.3e-221
Identity = 420/822 (51.09%), Postives = 531/822 (64.60%), Query Frame = 0

Query: 23  NGDWLVGVGSFDMTGPAAQVNMMGYANMDQNTAGVHFRLRARTFVVAKTVDGPRFAFVNL 82
           + D+L+G+GS+D+TGPAA VNMMGYANM+Q  +GVHFRLRAR F+VA+     R AFVNL
Sbjct: 26  DSDYLMGLGSYDITGPAADVNMMGYANMEQVASGVHFRLRARAFIVAEPYK-KRIAFVNL 85

Query: 83  DAGMASQLVTIKVLERLKSRFGDLYTEENVAISGIHTHAGPGGYLQYLVYSITSLGFVQQ 142
           DAGMASQLVTIKV+ERLK R+G+LYTEENVAISG HTHAGPGGYLQY++Y +TSLGFV Q
Sbjct: 86  DAGMASQLVTIKVIERLKQRYGELYTEENVAISGTHTHAGPGGYLQYILYLVTSLGFVHQ 145

Query: 143 SFDAIVNAIEQSIIQAHQSLKPGSIFINKGDVVNAGINRSPSAYLLNPQEERARYSTNVD 202
           SF+A+V+ IEQSIIQAH++L+PGSI INKG++++AG+NRSPSAYL NP  ER++Y  +VD
Sbjct: 146 SFNALVDGIEQSIIQAHENLRPGSILINKGELLDAGVNRSPSAYLNNPAHERSKYEYDVD 205

Query: 203 TEMTVLKFVDGASGKSVGAFSWFPTHGTSMSRDNKLISGDNKGAAARFFEDWIASKRTKR 262
            EMT++KFVD                             D  G  AR  EDW        
Sbjct: 206 KEMTLVKFVD-----------------------------DQWGPVARIMEDWF------- 265

Query: 263 SSNNNASDFNEMGREIFLSCQDFNEMGREILLKLLSVGVGEKSASELGRNGGIGGSELIG 322
                                                                       
Sbjct: 266 ------------------------------------------------------------ 325

Query: 323 VRSEGERNDGIGGSELVGVRSGRETTASAATALVDIVELMKKAETIKATGGKRCSKASSQ 382
                ER +   G   V V S R  ++  +       +LM+ A ++ +TGGK  ++ SS 
Sbjct: 326 -----EREN---GCRSVDVESPRRVSSIISDPYDQ--DLMEMASSLLSTGGKTVTRMSSV 385

Query: 383 ASKVRNN----DGSLFVAAFCQSNVGDVTPNVLGAFCTDSGNPCDFNRSSCHGNDLLCVG 442
           A +VR+     D   FV+AFCQ+N GDV+PNVLGAFC D+G PC+FN+S+C G +  C G
Sbjct: 386 ARRVRSRFRHADKPRFVSAFCQTNCGDVSPNVLGAFCIDTGLPCEFNQSTCGGKNEQCYG 445

Query: 443 RGPGFPDEILSTKIIGERQFLKAADLFTTATEKLTGEIDFRHVYLNFTDIEVELDGDN-- 502
           RGPG+PDE  ST+IIGERQF KAADLFT A+E++ G++D+RH Y++F+ +EV ++G N  
Sbjct: 446 RGPGYPDEFESTRIIGERQFKKAADLFTKASEEIQGKVDYRHAYVDFSQLEVTINGQNGG 505

Query: 503 --VVRTCPAALGPGFAAGTTDGPGVFGFQQGDTKINELWRKLRDSLRKPSEFQVGCQKPK 562
             VV+TCPAA+G GFAAGTTDGPG F F+QGD + N  WR +R+ L+ P+E QV CQ+PK
Sbjct: 506 SEVVKTCPAAMGFGFAAGTTDGPGAFDFKQGDDQGNPFWRLVRNLLKNPTEEQVRCQRPK 565

Query: 563 TVLLDTGEMFEPYAWAPAILPIQILRLGKLIILSVPGEFTTMAGRRLREAVKETLISNGN 622
            +LLDTGEM +PY WAP+ILP+QILR+G+L+IL VPGEFTTMAGRRLR+AVK  L    N
Sbjct: 566 PILLDTGEMKQPYDWAPSILPVQILRIGQLVILCVPGEFTTMAGRRLRDAVKTVLKEGSN 625

Query: 623 GEFDDDTHVVIAGLTNTYSQYVATFEEYEQQRYEAASTLYGPHTLSAYIQEFKKLATAIA 682
           G    +  VVIAGLTN+YSQY+ATFEEY+ QRYE ASTLYGPHTLS YIQEFKKLA  + 
Sbjct: 626 GR---EFSVVIAGLTNSYSQYIATFEEYQVQRYEGASTLYGPHTLSGYIQEFKKLANDLL 685

Query: 683 KGENIVKPIASPPDLSSVQLRFVLEPFGESPPNGINFGDIQQDIKLPKTGWFKRGDKQKP 742
             +    P   PPDL   Q+  +     +  P G  FGD+  D+  P+   F++G     
Sbjct: 686 SAQT-TDPGPQPPDLLHKQISLLTPVVADMTPIGTAFGDVTSDV--PRLSKFRKG-ADIV 732

Query: 743 TATFWTANPRFDLLTEGTYAVVER-LE-KQRWKPAYDDDDFSLLFKWTLDNNSFINSLAT 802
              F +ANPR DL+TEGT+A+VER LE ++ W P YDDDDF L FKW+        S AT
Sbjct: 746 RVQFRSANPRNDLMTEGTFALVERWLEGRETWVPVYDDDDFCLRFKWSRPFKLSTQSTAT 732

Query: 803 IEWDIPTDADPGVYRLRHFGSSKTTTNSSNIYFSGASRAFAV 835
           IEW IP  A PGVYR+ HFGS+KT  +S + +FSG+S AF V
Sbjct: 806 IEWRIPETASPGVYRITHFGSAKTPISSIH-HFSGSSSAFVV 732