Lag0004066 (gene) Sponge gourd (AG‐4) v1

BLAST of Lag0004066 vs. NCBI nr
Match: XP_022153149.1 (1,4-alpha-glucan-branching enzyme 1, chloroplastic/amyloplastic-like [Momordica charantia])

HSP 1 Score: 1513.0 bits (3916), Expect = 0.0e+00
Identity = 725/842 (86.10%), Postives = 772/842 (91.69%), Query Frame = 0

Query: 1   MSTSPCVLHSTALASLPLSAKKNSNLDSRDQWSSLRQQIELNFGSRVSHRRQKNPFVRPF 60
           M  S CVL  T+LASL LSAKKN +  SR++W SLR+QIEL F SR+SH R  NPF+ P 
Sbjct: 1   MLASSCVLPPTSLASLSLSAKKNFSAGSRNKWPSLRRQIELIFVSRMSHHRHMNPFMHPI 60

Query: 61  STYKRVHSSAISSVMTDDTSTVSSADESEENIGILSHDPGLQPFKDHFKYRVGRYADLLN 120
           STYKR HSSAIS VMTD+  T++SADE  EN+GILSHDPGL+PFKDHFKYR+GRYA+LL+
Sbjct: 61  STYKRGHSSAISCVMTDNAPTITSADEIMENMGILSHDPGLEPFKDHFKYRIGRYANLLS 120

Query: 121 LLEKYEGGLDEFARGYLKFGFNREEGGIVYREWAPAAQEAQIIGDFNGWDGSNHQMEKNE 180
           LLEK+EGGLDEFA+GYLKFGFNREEGGIVYREWAPAAQEAQIIGDFNGWDGSNHQMEKNE
Sbjct: 121 LLEKHEGGLDEFAQGYLKFGFNREEGGIVYREWAPAAQEAQIIGDFNGWDGSNHQMEKNE 180

Query: 181 FGTWSIKIPDLEGKPAIAHNSRVKFRFKHGNGVWIDRIPAWIKYATVDPKRFAAPYNGVY 240
           FG WSIK+PD  GKPAI HNSRVKFRFKHGNGVWIDRIPAWI+YATVDP RFAAPY+GVY
Sbjct: 181 FGIWSIKLPDNGGKPAITHNSRVKFRFKHGNGVWIDRIPAWIRYATVDPTRFAAPYDGVY 240

Query: 241 WDPPALERYEFKHPRPTKPEAPRVYEAHVGMSSSEPRVSSYREFADFVLPRIKENNYNTV 300
           WDPP LERYEF+HPRP KPEAPR+YEAHVGMSSSEPRV+SYREFADF+LPRIKENNYNTV
Sbjct: 241 WDPPPLERYEFEHPRPAKPEAPRIYEAHVGMSSSEPRVNSYREFADFILPRIKENNYNTV 300

Query: 301 QLMAIMEHSYYASFGYHVTNFFAVSSRSGTPEDLKYLIDKAHGLGIRVLMDVVHSHASNN 360
           QLMAIMEHSYYASFGYHVTNFFAVSSRSGTPEDLKYLIDKAHGLG+RVLMDV+HSHASNN
Sbjct: 301 QLMAIMEHSYYASFGYHVTNFFAVSSRSGTPEDLKYLIDKAHGLGLRVLMDVIHSHASNN 360

Query: 361 VTDGLNGFDVGQGTQDSYFHTGDRGYHKLWDSRIFNYANWEVLRFLLSNIRWWLEEYQFD 420
           VTDGLNGFDVGQ +Q SYFHTGDRGYHKLWDSR+FNYANWEVLRFLLSNIRWWLEEY+FD
Sbjct: 361 VTDGLNGFDVGQSSQGSYFHTGDRGYHKLWDSRLFNYANWEVLRFLLSNIRWWLEEYKFD 420

Query: 421 GFRFDGVTSMLYHHHGINMAFTGNYNEYFSEATDVDAVVYLMLANNLIHSIFPDATVIAE 480
           GFRFDGVTSMLYHHHGINM FTGNYNEYFSEATDVDAVVYLMLANNLIHSI PDATVIAE
Sbjct: 421 GFRFDGVTSMLYHHHGINMGFTGNYNEYFSEATDVDAVVYLMLANNLIHSILPDATVIAE 480

Query: 481 DVSGMPGLGRPVSEGGIGFDYRLQMAIPDKWIDYLKNKTDEEWSMGEISWNLTNRRYSEK 540
           DVSGMPGLGRPVSEGGIGFDYRLQMAIPDKWIDYLKNKTDEEWSMGEISWNLTNRRYSEK
Sbjct: 481 DVSGMPGLGRPVSEGGIGFDYRLQMAIPDKWIDYLKNKTDEEWSMGEISWNLTNRRYSEK 540

Query: 541 CISYAESHDQAIVGDKTISFLLMDKEMYSGMSCLTDASPIVERGIALHKMIHFITMALGG 600
           CISYAESHDQAIVGDKT++FLLMDKEMYSGMSCLT+ASPIVERGIALHKMIHFITMALGG
Sbjct: 541 CISYAESHDQAIVGDKTVAFLLMDKEMYSGMSCLTEASPIVERGIALHKMIHFITMALGG 600

Query: 601 DGYLNFMGNEFGHPEWIDFPREGNGWSYDKCRRQWNLPDTDHLRYKFLNAFDRAMNLLDD 660
           +GYLNFMGNEFGHPEWIDFPREGNGWSYDKCRRQWNLPDTDHLRYKFLNAFDRAMNLLDD
Sbjct: 601 EGYLNFMGNEFGHPEWIDFPREGNGWSYDKCRRQWNLPDTDHLRYKFLNAFDRAMNLLDD 660

Query: 661 KFSFLASSKQIVSWTGEEDKVIIFERGDLVFVFNFHPVNTYDGYKVGCDLPGKYRVALDS 720
           KFSFLASSKQI+S T EEDKVI+FERGDLVFVFNFHPVNTYDGYKVGCDLPGKYRVALDS
Sbjct: 661 KFSFLASSKQILSCTSEEDKVIVFERGDLVFVFNFHPVNTYDGYKVGCDLPGKYRVALDS 720

Query: 721 DARDFGGLGRVGHDVDHFTSPEGIPGVPETNFNNRPNSFKVLSPARTCVVYHRVDESKEE 780
           DA DFGG GR+GHD DHFTSPEGIPGVPETNFNNRPNSFKVLSPARTCVVY+RVDESKE+
Sbjct: 721 DAWDFGGHGRIGHDADHFTSPEGIPGVPETNFNNRPNSFKVLSPARTCVVYYRVDESKEK 780

Query: 781 IENGDLVGVNKPVVTEVSTRLGRSE------KENGD-SVGANKPIAIDVSTQLGHCEKEN 836
            ENGD VG NKP+ T+VS R    E       E GD +VG  +   ++    +   + E+
Sbjct: 781 -ENGDSVGTNKPLTTDVSARHTEEEDEELTHSEEGDIAVGETRTTNVE-DDNIDTSKSED 840

BLAST of Lag0004066 vs. NCBI nr
Match: XP_038884508.1 (1,4-alpha-glucan-branching enzyme 1, chloroplastic/amyloplastic-like [Benincasa hispida])

HSP 1 Score: 1513.0 bits (3916), Expect = 0.0e+00
Identity = 735/843 (87.19%), Postives = 770/843 (91.34%), Query Frame = 0

Query: 1   MSTSPCVLHSTALASLPLSAKKNSNLDSRDQWSSLRQQIELNFGSRVSHRRQKNPFVRPF 60
           M  SPCVL S ALASL LSAKK   LDSR+QW SLRQ  ELNFGS++S+ RQ NPF+ PF
Sbjct: 1   MLPSPCVLPSPALASLSLSAKKIFTLDSRNQWPSLRQ--ELNFGSKMSNHRQMNPFMLPF 60

Query: 61  STYKRVHSSAISSVMTDDTSTVSSADESEENIGILSHDPGLQPFKDHFKYRVGRYADLLN 120
           STYK+VHS AISSV+TDDT  VSS DES ENIGILSHDPGL+ FKDHFKYRVGRYADLLN
Sbjct: 61  STYKKVHSPAISSVVTDDTPKVSSPDESTENIGILSHDPGLESFKDHFKYRVGRYADLLN 120

Query: 121 LLEKYEGGLDEFARGYLKFGFNREEGGIVYREWAPAAQEAQIIGDFNGWDGSNHQMEKNE 180
           LL+K+EGGLDEFARGYLKFGFNREE GIVYREWAPAAQEAQIIGDFNGWDG+NH+MEKNE
Sbjct: 121 LLDKHEGGLDEFARGYLKFGFNREEDGIVYREWAPAAQEAQIIGDFNGWDGTNHRMEKNE 180

Query: 181 FGTWSIKIPDLEGKPAIAHNSRVKFRFKHGNGVWIDRIPAWIKYATVDPKRFAAPYNGVY 240
           FG WSIKI DL GKPAI+HNSRVKFRFKHGNGVWIDRIPAWIKYATVDP +FAAPY+GVY
Sbjct: 181 FGIWSIKIRDLSGKPAISHNSRVKFRFKHGNGVWIDRIPAWIKYATVDPTKFAAPYDGVY 240

Query: 241 WDPPALERYEFKHPRPTKPEAPRVYEAHVGMSSSEPRVSSYREFADFVLPRIKENNYNTV 300
           WDPP LERYEFKHPRP KP+APRVYEAHVGMSSSEPRVSSYREFADFVLPRIKENNYNTV
Sbjct: 241 WDPPPLERYEFKHPRPAKPKAPRVYEAHVGMSSSEPRVSSYREFADFVLPRIKENNYNTV 300

Query: 301 QLMAIMEHSYYASFGYHVTNFFAVSSRSGTPEDLKYLIDKAHGLGIRVLMDVVHSHASNN 360
           QLMAIMEHSYYASFGYHVTNFFAVSSRSGTPEDLKYLIDKAHGLG+RVLMDVVHSHASNN
Sbjct: 301 QLMAIMEHSYYASFGYHVTNFFAVSSRSGTPEDLKYLIDKAHGLGLRVLMDVVHSHASNN 360

Query: 361 VTDGLNGFDVGQGTQDSYFHTGDRGYHKLWDSRIFNYANWEVLRFLLSNIRWWLEEYQFD 420
           VTDGLNGFDVGQ TQDSYFHTGDRGYHKLWDSR+FNYANWEVLRFLLSNIRWWLEEYQFD
Sbjct: 361 VTDGLNGFDVGQSTQDSYFHTGDRGYHKLWDSRLFNYANWEVLRFLLSNIRWWLEEYQFD 420

Query: 421 GFRFDGVTSMLYHHHGINMAFTGNYNEYFSEATDVDAVVYLMLANNLIHSIFPDATVIAE 480
           GFRFDGVTSMLYHHHGINMAFTG YNEYFSEATDVDAVVYLMLANNL HSI PDATVIAE
Sbjct: 421 GFRFDGVTSMLYHHHGINMAFTGTYNEYFSEATDVDAVVYLMLANNLTHSILPDATVIAE 480

Query: 481 DVSGMPGLGRPVSEGGIGFDYRLQMAIPDKWIDYLKNKTDEEWSMGEISWNLTNRRYSEK 540
           DVSGMPGLGRPVSEGGIGFDYRLQMAIPDKWIDYLKNK+DEEWSMGEISWNLTNRRYSEK
Sbjct: 481 DVSGMPGLGRPVSEGGIGFDYRLQMAIPDKWIDYLKNKSDEEWSMGEISWNLTNRRYSEK 540

Query: 541 CISYAESHDQAIVGDKTISFLLMDKEMYSGMSCLTDASPIVERGIALHKMIHFITMALGG 600
           CISYAESHDQ+IVGDKTI+FLLMDKEMYSGMSCLTDASP+VERGI+LHKMIHFITMALGG
Sbjct: 541 CISYAESHDQSIVGDKTIAFLLMDKEMYSGMSCLTDASPVVERGISLHKMIHFITMALGG 600

Query: 601 DGYLNFMGNEFGHPEWIDFPREGNGWSYDKCRRQWNLPDTDHLRYKFLNAFDRAMNLLDD 660
           +GYLNFMGNEFGHPEWIDFPREGNGWSYDKCRRQWNLPDTDHLRYKFLNAFDRAMN LDD
Sbjct: 601 EGYLNFMGNEFGHPEWIDFPREGNGWSYDKCRRQWNLPDTDHLRYKFLNAFDRAMNSLDD 660

Query: 661 KFSFLASSKQIVSWTGEEDKVIIFERGDLVFVFNFHPVNTYDGYKVGCDLPGKYRVALDS 720
           KFSFLASSKQIVS T EEDKVI+FERGDLVFVFNFHP+NTYDGYKVGCDLPGKYRVALDS
Sbjct: 661 KFSFLASSKQIVSCTSEEDKVIVFERGDLVFVFNFHPINTYDGYKVGCDLPGKYRVALDS 720

Query: 721 DARDFGGLGRVGHDVDHFTSPEGIPGVPETNFNNRPNSFKVLSPARTCVVYHRVDESKEE 780
           DA DFGG GRVGHDVDHFTSPEGIPGV ETNFNNRPNSFKVLSPARTCVVYHRVDESKE+
Sbjct: 721 DAWDFGGHGRVGHDVDHFTSPEGIPGVSETNFNNRPNSFKVLSPARTCVVYHRVDESKEK 780

Query: 781 IENGDLVGVNKPVVTEVSTR--------LGRSEKENGDSVGANKPIAIDVSTQLGHCEKE 836
            EN +LVG N+   T+V  R            E+EN  + G    I  D  T+    + E
Sbjct: 781 -ENDNLVGANEARATDVFARHVEDDNEGSAGGEEENDIAAGEISKIEDDTDTR----KTE 836

BLAST of Lag0004066 vs. NCBI nr
Match: XP_008464849.1 (PREDICTED: 1,4-alpha-glucan-branching enzyme 1, chloroplastic/amyloplastic-like isoform X1 [Cucumis melo] >ADN33992.1 starch branching enzyme [Cucumis melo subsp. melo])

HSP 1 Score: 1508.4 bits (3904), Expect = 0.0e+00
Identity = 733/847 (86.54%), Postives = 771/847 (91.03%), Query Frame = 0

Query: 1   MSTSPCVLHSTALASLPLSAKKNSNLDSRDQWSSLRQQIELNFGSRVSHRRQKNPFVRPF 60
           M  SPCVL STA+ASL LSAKK    DSR+QW SLRQQ ELNF SR+SH RQ NPF+ P+
Sbjct: 1   MLLSPCVLPSTAIASLSLSAKKIFISDSRNQWPSLRQQTELNFWSRMSHHRQMNPFIFPY 60

Query: 61  STYKRVHSSAISSVMTDDTSTVSSADESEENIGILSHDPGLQPFKDHFKYRVGRYADLLN 120
           STYKRVHS AISSVMTDDTSTVSS DES ENIGILSHDPGL+PFKDHFKYRVGRY DLLN
Sbjct: 61  STYKRVHSPAISSVMTDDTSTVSSNDESMENIGILSHDPGLKPFKDHFKYRVGRYTDLLN 120

Query: 121 LLEKYEGGLDEFARGYLKFGFNREEGGIVYREWAPAAQEAQIIGDFNGWDGSNHQMEKNE 180
           LL+K+EGGLDEFA+GYLKFGFNREE GIVYREWAPAAQEAQI+GDFNGW+G+NH MEKNE
Sbjct: 121 LLDKHEGGLDEFAQGYLKFGFNREEDGIVYREWAPAAQEAQIVGDFNGWNGTNHCMEKNE 180

Query: 181 FGTWSIKIPDLEGKPAIAHNSRVKFRFKHGNGVWIDRIPAWIKYATVDPKRFAAPYNGVY 240
           FG WSIKI DL GKPAI+HNSRVKFRFKHGNGVWIDRIPAWIKYATVDP +FAAPY+GVY
Sbjct: 181 FGIWSIKIYDLGGKPAISHNSRVKFRFKHGNGVWIDRIPAWIKYATVDPTKFAAPYDGVY 240

Query: 241 WDPPALERYEFKHPRPTKPEAPRVYEAHVGMSSSEPRVSSYREFADFVLPRIKENNYNTV 300
           WDPP LERYEFKHPRP KP  PRVYEAHVGMSSSEPRVSSYREFADFVLPRIKENNYNTV
Sbjct: 241 WDPPPLERYEFKHPRPAKPNGPRVYEAHVGMSSSEPRVSSYREFADFVLPRIKENNYNTV 300

Query: 301 QLMAIMEHSYYASFGYHVTNFFAVSSRSGTPEDLKYLIDKAHGLGIRVLMDVVHSHASNN 360
           QLMAIMEHSYYASFGYHVTNFFAVSSRSGTPEDLKYLIDKAHGLG+RVLMDVVHSHASNN
Sbjct: 301 QLMAIMEHSYYASFGYHVTNFFAVSSRSGTPEDLKYLIDKAHGLGLRVLMDVVHSHASNN 360

Query: 361 VTDGLNGFDVGQGTQDSYFHTGDRGYHKLWDSRIFNYANWEVLRFLLSNIRWWLEEYQFD 420
           VTDGLNGFDVGQ TQDSYFHTGDRGYHKLWDSR+FNYANWEVLRFLLSNIRWWLEEYQFD
Sbjct: 361 VTDGLNGFDVGQSTQDSYFHTGDRGYHKLWDSRLFNYANWEVLRFLLSNIRWWLEEYQFD 420

Query: 421 GFRFDGVTSMLYHHHGINMAFTGNYNEYFSEATDVDAVVYLMLANNLIHSIFPDATVIAE 480
           GFRFDGVTSMLYHHHGI+MAFTGNYNEYFSEATDVDAVVYLMLANNL HSI PDATVIAE
Sbjct: 421 GFRFDGVTSMLYHHHGISMAFTGNYNEYFSEATDVDAVVYLMLANNLTHSILPDATVIAE 480

Query: 481 DVSGMPGLGRPVSEGGIGFDYRLQMAIPDKWIDYLKNKTDEEWSMGEISWNLTNRRYSEK 540
           DVSGMPGLGRPV EGGIGFDYRLQMAIPDKWIDYLKNK+DEEWSMGEISWNLTNRRYSEK
Sbjct: 481 DVSGMPGLGRPVFEGGIGFDYRLQMAIPDKWIDYLKNKSDEEWSMGEISWNLTNRRYSEK 540

Query: 541 CISYAESHDQAIVGDKTISFLLMDKEMYSGMSCLTDASPIVERGIALHKMIHFITMALGG 600
           CISYAESHDQ+IVGDKTI+FLLMDKEMYSGMSCL +ASP+VERGIALHKMIHFITMALGG
Sbjct: 541 CISYAESHDQSIVGDKTIAFLLMDKEMYSGMSCLENASPVVERGIALHKMIHFITMALGG 600

Query: 601 DGYLNFMGNEFGHPEWIDFPREGNGWSYDKCRRQWNLPDTDHLRYKFLNAFDRAMNLLDD 660
           +GYLNFMGNEFGHPEWIDFPREGNGWSYDKCRRQWNL DTDHLRYKFLNAFDRAMN LD+
Sbjct: 601 EGYLNFMGNEFGHPEWIDFPREGNGWSYDKCRRQWNLADTDHLRYKFLNAFDRAMNALDE 660

Query: 661 KFSFLASSKQIVSWTGEEDKVIIFERGDLVFVFNFHPVNTYDGYKVGCDLPGKYRVALDS 720
           KFSFLASSKQIVSWTGEEDKVI+FERGDLVFVFNFHP+NTYDGYKVGCDLPGKYRVALDS
Sbjct: 661 KFSFLASSKQIVSWTGEEDKVIVFERGDLVFVFNFHPINTYDGYKVGCDLPGKYRVALDS 720

Query: 721 DARDFGGLGRVGHDVDHFTSPEGIPGVPETNFNNRPNSFKVLSPARTCVVYHRVDESKEE 780
           DA DFGG GRVGHD+DHFTSPEGIPGVPETNFNNRPNSFKVLSPARTCVVY++VDESKE+
Sbjct: 721 DAGDFGGYGRVGHDIDHFTSPEGIPGVPETNFNNRPNSFKVLSPARTCVVYYKVDESKEK 780

Query: 781 IENGDLVG-----VNKPVVTEVSTRLGRSEKENGDSVG-ANKPIAIDVSTQLGHCEKENG 840
            E  DLV      V+   V E S  L   E+EN  +VG  +K    D+ T      K   
Sbjct: 781 -EKDDLVASVHEDVSARHVEEDSEGLAGREEENDIAVGKISKTEDDDIDT-----SKTEE 840

Query: 841 DSVGANE 842
           D V +N+
Sbjct: 841 DDVDSNK 841

BLAST of Lag0004066 vs. NCBI nr
Match: XP_004144236.1 (1,4-alpha-glucan-branching enzyme 1, chloroplastic/amyloplastic [Cucumis sativus] >KGN47537.1 hypothetical protein Csa_019021 [Cucumis sativus])

HSP 1 Score: 1506.5 bits (3899), Expect = 0.0e+00
Identity = 731/847 (86.30%), Postives = 772/847 (91.15%), Query Frame = 0

Query: 1   MSTSPCVLHSTALASLPLSAKKNSNLDSRDQWSSLRQQIELNFGSRVSHRRQKNPFVRPF 60
           M  S CVL STA+ASL LSAKK    DSR+QW SLRQQ ELNF SR+SH RQ NPFV P+
Sbjct: 1   MLLSSCVLPSTAVASLSLSAKKIIISDSRNQWPSLRQQTELNFWSRMSHHRQMNPFVFPY 60

Query: 61  STYKRVHSSAISSVMTDDTSTVSSADESEENIGILSHDPGLQPFKDHFKYRVGRYADLLN 120
           STYKRVHS AISSVMT+DTSTVSS DES ENIGILSHDPGL+PFKDHFKYRVGRY DLLN
Sbjct: 61  STYKRVHSPAISSVMTEDTSTVSSTDESMENIGILSHDPGLKPFKDHFKYRVGRYTDLLN 120

Query: 121 LLEKYEGGLDEFARGYLKFGFNREEGGIVYREWAPAAQEAQIIGDFNGWDGSNHQMEKNE 180
           LL+K+EGGLDEFARGYLKFGFNREE GIVYREWAPAAQEAQI+GDFNGWDG+NH MEKNE
Sbjct: 121 LLDKHEGGLDEFARGYLKFGFNREEDGIVYREWAPAAQEAQIVGDFNGWDGTNHCMEKNE 180

Query: 181 FGTWSIKIPDLEGKPAIAHNSRVKFRFKHGNGVWIDRIPAWIKYATVDPKRFAAPYNGVY 240
           FG WSIK+ DL GKPAI+HNSRVKFRFKHGNGVWIDRIPAWIKYATVDP +FAAPY+GVY
Sbjct: 181 FGIWSIKVYDLGGKPAISHNSRVKFRFKHGNGVWIDRIPAWIKYATVDPTKFAAPYDGVY 240

Query: 241 WDPPALERYEFKHPRPTKPEAPRVYEAHVGMSSSEPRVSSYREFADFVLPRIKENNYNTV 300
           WDPP LERYEFKHPRP KP APRVYEAHVGMSSSEPRV+SYREFADFVLPRIKENNYNTV
Sbjct: 241 WDPPPLERYEFKHPRPAKPNAPRVYEAHVGMSSSEPRVNSYREFADFVLPRIKENNYNTV 300

Query: 301 QLMAIMEHSYYASFGYHVTNFFAVSSRSGTPEDLKYLIDKAHGLGIRVLMDVVHSHASNN 360
           QLMAIMEHSYYASFGYH+TNFFAVSSRSGTPEDLKYLIDKAHGLG+RVLMDVVHSHASNN
Sbjct: 301 QLMAIMEHSYYASFGYHITNFFAVSSRSGTPEDLKYLIDKAHGLGLRVLMDVVHSHASNN 360

Query: 361 VTDGLNGFDVGQGTQDSYFHTGDRGYHKLWDSRIFNYANWEVLRFLLSNIRWWLEEYQFD 420
           VTDGLNGFDVGQ +QDSYFHTGDRGYHKLWDSR+FNYANWEVLRFLLSNIRWWLEEYQFD
Sbjct: 361 VTDGLNGFDVGQSSQDSYFHTGDRGYHKLWDSRLFNYANWEVLRFLLSNIRWWLEEYQFD 420

Query: 421 GFRFDGVTSMLYHHHGINMAFTGNYNEYFSEATDVDAVVYLMLANNLIHSIFPDATVIAE 480
           GFRFDGVTSMLYHHHGINM F+GNYNEYFSEATDVDAVVYLMLANNL HSI PDATVIAE
Sbjct: 421 GFRFDGVTSMLYHHHGINMGFSGNYNEYFSEATDVDAVVYLMLANNLTHSILPDATVIAE 480

Query: 481 DVSGMPGLGRPVSEGGIGFDYRLQMAIPDKWIDYLKNKTDEEWSMGEISWNLTNRRYSEK 540
           DVSGMPGLGRPV EGGIGFDYRLQMAIPDKWIDYLKNK+DEEWSMGEISWNLTNRRYSEK
Sbjct: 481 DVSGMPGLGRPVFEGGIGFDYRLQMAIPDKWIDYLKNKSDEEWSMGEISWNLTNRRYSEK 540

Query: 541 CISYAESHDQAIVGDKTISFLLMDKEMYSGMSCLTDASPIVERGIALHKMIHFITMALGG 600
           CISYAESHDQ+IVGDKTI+FLLMDKEMYSGMSCL +ASP+VERGIALHKMIHFITMALGG
Sbjct: 541 CISYAESHDQSIVGDKTIAFLLMDKEMYSGMSCLENASPVVERGIALHKMIHFITMALGG 600

Query: 601 DGYLNFMGNEFGHPEWIDFPREGNGWSYDKCRRQWNLPDTDHLRYKFLNAFDRAMNLLDD 660
           +GYLNFMGNEFGHPEWIDFPREGNGWSYDKCRRQWNLPDTDHLRYKFLNAFD AMN LD+
Sbjct: 601 EGYLNFMGNEFGHPEWIDFPREGNGWSYDKCRRQWNLPDTDHLRYKFLNAFDSAMNALDE 660

Query: 661 KFSFLASSKQIVSWTGEEDKVIIFERGDLVFVFNFHPVNTYDGYKVGCDLPGKYRVALDS 720
           KFSFLASSKQIVSWTGEEDKVI+FERGDLVFVFNFHPVNTYDGYKVGCDLPGKYRVALDS
Sbjct: 661 KFSFLASSKQIVSWTGEEDKVIVFERGDLVFVFNFHPVNTYDGYKVGCDLPGKYRVALDS 720

Query: 721 DARDFGGLGRVGHDVDHFTSPEGIPGVPETNFNNRPNSFKVLSPARTCVVYHRVDESKEE 780
           DA DFGG GRVGHD+DHFTSPEGIPGVPETNFNNRPNSFK+LSPARTCVVY++VDESKE+
Sbjct: 721 DASDFGGHGRVGHDIDHFTSPEGIPGVPETNFNNRPNSFKILSPARTCVVYYKVDESKEK 780

Query: 781 IENGDLVG-VNKPV----VTEVSTRLGRSEKENGDSVG-ANKPIAIDVSTQLGHCEKENG 840
            E  DLVG  N+ V    V E S  L   ++EN  +VG  +K    D+ T      K   
Sbjct: 781 -EKDDLVGSANEDVFARHVEEDSEGLAGCKEENDIAVGEISKTEDDDIDT-----SKPED 840

Query: 841 DSVGANE 842
           D V +N+
Sbjct: 841 DDVDSNK 841

BLAST of Lag0004066 vs. NCBI nr
Match: KAG6599100.1 (1,4-alpha-glucan-branching enzyme 1, chloroplastic/amyloplastic, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 1489.9 bits (3856), Expect = 0.0e+00
Identity = 711/814 (87.35%), Postives = 755/814 (92.75%), Query Frame = 0

Query: 1   MSTSPCVLHSTALASLPLSAKKNSNLDSRDQWSSLRQQIELNFGSRVSHRRQKNPFVRPF 60
           M  SPCVL STALASL LSAKKN   DSR+Q SSLRQQ  LNFGSR+SH RQ +PF+ PF
Sbjct: 1   MLASPCVLPSTALASLSLSAKKNFTSDSRNQCSSLRQQFVLNFGSRMSHHRQMHPFMAPF 60

Query: 61  STYKRVHSSA-ISSVMTDDTSTVSSADESEENIGILSHDPGLQPFKDHFKYRVGRYADLL 120
           STYKRVHS A ISSVMTDDTSTVSSADE+ ++IGIL HDP L+PFKDHFKYR+GRY+D+L
Sbjct: 61  STYKRVHSPAIISSVMTDDTSTVSSADENMKDIGILRHDPSLEPFKDHFKYRIGRYSDVL 120

Query: 121 NLLEKYEGGLDEFARGYLKFGFNREEGGIVYREWAPAAQEAQIIGDFNGWDGSNHQMEKN 180
           NL +K+EGGLDEFARGYLKFGFNRE  GIVYREWAP AQEAQIIGDFNGWDGSNH+MEKN
Sbjct: 121 NLFDKHEGGLDEFARGYLKFGFNREGDGIVYREWAPVAQEAQIIGDFNGWDGSNHRMEKN 180

Query: 181 EFGTWSIKIPDLEGKPAIAHNSRVKFRFKHGNGVWIDRIPAWIKYATVDPKRFAAPYNGV 240
           EFG WSI IPD++GKPAIAHNSRVKFRFKHGNG WIDRIPAWIKYAT DP RF APY+GV
Sbjct: 181 EFGIWSINIPDIDGKPAIAHNSRVKFRFKHGNGAWIDRIPAWIKYATADPTRFGAPYDGV 240

Query: 241 YWDPPALERYEFKHPRPTKPEAPRVYEAHVGMSSSEPRVSSYREFADFVLPRIKENNYNT 300
           YW+PP LERYEFKHPRP KPEAPR+YEAHVGMSSSEPRV+SYREFADFVLPRIKENNYNT
Sbjct: 241 YWEPPPLERYEFKHPRPAKPEAPRIYEAHVGMSSSEPRVNSYREFADFVLPRIKENNYNT 300

Query: 301 VQLMAIMEHSYYASFGYHVTNFFAVSSRSGTPEDLKYLIDKAHGLGIRVLMDVVHSHASN 360
           VQLMAIMEHSYYASFGYHVTNFFA+SSRSGTPEDLKYLIDKAHGLG+RVLMD+VHSHASN
Sbjct: 301 VQLMAIMEHSYYASFGYHVTNFFAISSRSGTPEDLKYLIDKAHGLGLRVLMDIVHSHASN 360

Query: 361 NVTDGLNGFDVGQGTQDSYFHTGDRGYHKLWDSRIFNYANWEVLRFLLSNIRWWLEEYQF 420
           NVTDGLNGFDVGQ TQDSYFHTGDRGYHKLWDSR+FNYANWEVLRFLLSNIRWWLEEYQF
Sbjct: 361 NVTDGLNGFDVGQSTQDSYFHTGDRGYHKLWDSRLFNYANWEVLRFLLSNIRWWLEEYQF 420

Query: 421 DGFRFDGVTSMLYHHHGINMAFTGNYNEYFSEATDVDAVVYLMLANNLIHSIFPDATVIA 480
           DGFRFDGVTSMLYHHHGIN AFTGNYNEYFSEATDVDAVVYLMLANNLIHSI P+ATVIA
Sbjct: 421 DGFRFDGVTSMLYHHHGINTAFTGNYNEYFSEATDVDAVVYLMLANNLIHSILPNATVIA 480

Query: 481 EDVSGMPGLGRPVSEGGIGFDYRLQMAIPDKWIDYLKNKTDEEWSMGEISWNLTNRRYSE 540
           EDVSGMPGLG PVSEGGIGFDYRLQMAIPDKWIDY+KNK DEEWSMGEISWNLTNRRYSE
Sbjct: 481 EDVSGMPGLGIPVSEGGIGFDYRLQMAIPDKWIDYVKNKNDEEWSMGEISWNLTNRRYSE 540

Query: 541 KCISYAESHDQAIVGDKTISFLLMDKEMYSGMSCLTDASPIVERGIALHKMIHFITMALG 600
           KCISYAESHDQ+IVGDKTI+FLLMDKEMYSGMSCLT+ASP+VERGIALHKMIHFITMALG
Sbjct: 541 KCISYAESHDQSIVGDKTIAFLLMDKEMYSGMSCLTEASPVVERGIALHKMIHFITMALG 600

Query: 601 GDGYLNFMGNEFGHPEWIDFPREGNGWSYDKCRRQWNLPDTDHLRYKFLNAFDRAMNLLD 660
           G+GYLNFMGNEFGHPEWIDFPREGNGWSYDKCRRQWNLPDTDHLRYKFLNAFDRAMN LD
Sbjct: 601 GEGYLNFMGNEFGHPEWIDFPREGNGWSYDKCRRQWNLPDTDHLRYKFLNAFDRAMNSLD 660

Query: 661 DKFSFLASSKQIVSWTGEEDKVIIFERGDLVFVFNFHPVNTYDGYKVGCDLPGKYRVALD 720
           DKFSFLASSKQIVS T EE+KVI+FERGDLVFVFNFHP+NTYDGYKVGCD+PGKYRVALD
Sbjct: 661 DKFSFLASSKQIVSCTSEEEKVIVFERGDLVFVFNFHPINTYDGYKVGCDIPGKYRVALD 720

Query: 721 SDARDFGGLGRVGHDVDHFTSPEGIPGVPETNFNNRPNSFKVLSPARTCVVYHRVDESKE 780
           SDA +FGG GRVGHD+DHFTSPEGIPGVPETNFNNRPNSFKVLSPA+TCVVY+RV ESKE
Sbjct: 721 SDASEFGGRGRVGHDIDHFTSPEGIPGVPETNFNNRPNSFKVLSPAQTCVVYYRVYESKE 780

Query: 781 EIENGDLVGVNKPVVTEV---STRLGRSEKENGD 811
           + ENG  VG ++   T+V     + G   K  GD
Sbjct: 781 K-ENGVSVGASEAHATDVVLARDKEGEISKIEGD 813

BLAST of Lag0004066 vs. ExPASy Swiss-Prot
Match: Q41059 (1,4-alpha-glucan-branching enzyme 1, chloroplastic/amyloplastic (Fragment) OS=Pisum sativum OX=3888 GN=SBEII PE=1 SV=1)

HSP 1 Score: 1259.2 bits (3257), Expect = 0.0e+00
Identity = 608/800 (76.00%), Postives = 676/800 (84.50%), Query Frame = 0

Query: 31  QWSSLRQQIELNFGSRVSHRRQKNPFVRPFSTYKRVHSSAIS-SVMTDDTSTVSSADESE 90
           Q+ + ++ +EL  G +  +  +   F    S Y++V S     SVMTDD ST+ S +E  
Sbjct: 14  QYLAKQKPVELTLGYQNPNGCKVCSFGSKGSIYQKVSSGFKGVSVMTDDKSTMPSVEEDF 73

Query: 91  ENIGILSHDPGLQPFKDHFKYRVGRYADLLNLLEKYEGGLDEFARGYLKFGFNREEGGIV 150
           ENIGIL+ D  L+PFKDHFKYR+ RY     L+E+YEGGL EFA+GYLKFGFNREE GI 
Sbjct: 74  ENIGILNVDSSLEPFKDHFKYRLKRYLHQKKLIEEYEGGLQEFAKGYLKFGFNREEDGIS 133

Query: 151 YREWAPAAQEAQIIGDFNGWDGSNHQMEKNEFGTWSIKIPDLEGKPAIAHNSRVKFRFKH 210
           YREWAPAAQEAQIIGDFNGW+GSN  MEK++FG WSI+IPD +G PAI HNSRVKFRFKH
Sbjct: 134 YREWAPAAQEAQIIGDFNGWNGSNLHMEKDQFGVWSIQIPDADGNPAIPHNSRVKFRFKH 193

Query: 211 GNGVWIDRIPAWIKYATVDPKRFAAPYNGVYWDPPALERYEFKHPRPTKPEAPRVYEAHV 270
            +GVW+DRIPAWIKYATVDP RFAAPY+GVYWDPP  ERY+FKHPRP KP+APR+YEAHV
Sbjct: 194 SDGVWVDRIPAWIKYATVDPTRFAAPYDGVYWDPPLSERYQFKHPRPPKPKAPRIYEAHV 253

Query: 271 GMSSSEPRVSSYREFADFVLPRIKENNYNTVQLMAIMEHSYYASFGYHVTN-FFAVSSRS 330
           GMSSSEPR++SYREFAD VLPRI+ENNYNTVQLMA+MEHSYYASF YHVT  FFAVSSRS
Sbjct: 254 GMSSSEPRINSYREFADDVLPRIRENNYNTVQLMAVMEHSYYASFWYHVTKPFFAVSSRS 313

Query: 331 GTPEDLKYLIDKAHGLGIRVLMDVVHSHASNNVTDGLNGFDVGQGTQDSYFHTGDRGYHK 390
           G+PEDLKYLIDKAH LG+ VLMDV+HSHASNNVTDGLNGFDVGQ +Q SYFH GDRGYHK
Sbjct: 314 GSPEDLKYLIDKAHSLGLNVLMDVIHSHASNNVTDGLNGFDVGQSSQQSYFHAGDRGYHK 373

Query: 391 LWDSRIFNYANWEVLRFLLSNIRWWLEEYQFDGFRFDGVTSMLYHHHGINMAFTGNYNEY 450
           LWDSR+FNYANW+   FLLSN+RWWLEEY+FDGFRFDGVTSMLYHHHGINMAFTG+YNEY
Sbjct: 374 LWDSRLFNYANWK-SSFLLSNLRWWLEEYKFDGFRFDGVTSMLYHHHGINMAFTGDYNEY 433

Query: 451 FSEATDVDAVVYLMLANNLIHSIFPDATVIAEDVSGMPGLGRPVSEGGIGFDYRLQMAIP 510
           FSE TDVDAVVYLMLAN+L+H I PDAT IAEDVSGMPGLGRPVSE GIGFDYRL MAIP
Sbjct: 434 FSEETDVDAVVYLMLANSLVHDILPDATDIAEDVSGMPGLGRPVSEVGIGFDYRLAMAIP 493

Query: 511 DKWIDYLKNKTDEEWSMGEISWNLTNRRYSEKCISYAESHDQAIVGDKTISFLLMDKEMY 570
           DKWIDYLKNK D EWSM EIS NLTNRRY+EKC+SYAESHDQ+IVGDKTI+FLLMD+EMY
Sbjct: 494 DKWIDYLKNKKDSEWSMKEISLNLTNRRYTEKCVSYAESHDQSIVGDKTIAFLLMDEEMY 553

Query: 571 SGMSCLTDASPIVERGIALHKMIHFITMALGGDGYLNFMGNEFGHPEWIDFPREGNGWSY 630
           S MSCLT  SP +ERGI+LHKMIHFIT+ALGG+GYLNFMGNEFGHPEWIDFPREGNGWSY
Sbjct: 554 SSMSCLTMLSPTIERGISLHKMIHFITLALGGEGYLNFMGNEFGHPEWIDFPREGNGWSY 613

Query: 631 DKCR-RQWNLPDTDHLRYKFLNAFDRAMNLLDDKFSFLASSKQIVSWTGEEDKVIIFERG 690
           +KCR  QWNL DT+HLRYKF+NAFDRAMNLLDDKFS LAS+KQIVS T  EDKVI+FERG
Sbjct: 614 EKCRLTQWNLVDTNHLRYKFMNAFDRAMNLLDDKFSILASTKQIVSSTNNEDKVIVFERG 673

Query: 691 DLVFVFNFHPVNTYDGYKVGCDLPGKYRVALDSDARDFGGLGRVGHDVDHFTSPEGIPGV 750
           DLVFVFNFHP NTY+GYKVGCDLPGKYRVALDSDA +FGG GRVGHD D FTSPEGIPG+
Sbjct: 674 DLVFVFNFHPENTYEGYKVGCDLPGKYRVALDSDATEFGGHGRVGHDADQFTSPEGIPGI 733

Query: 751 PETNFNNRPNSFKVLSPARTCVVYHRVDESKEEIENGDLVGVNKPV------VTEVSTRL 810
           PETNFNNRPNSFKVLSP  TCVVY+RVDE +EE  N +L  V +        V  +    
Sbjct: 734 PETNFNNRPNSFKVLSPPHTCVVYYRVDERQEESNNPNLGSVEETFAAADTDVARIPDVS 793

Query: 811 GRSEKENGDSVGANKPIAID 822
             SE  N D +  N   A+D
Sbjct: 794 MESEDSNLDRIEDNSEDAVD 812

BLAST of Lag0004066 vs. ExPASy Swiss-Prot
Match: P30924 (1,4-alpha-glucan-branching enzyme OS=Solanum tuberosum OX=4113 GN=SBE1 PE=2 SV=2)

HSP 1 Score: 1235.3 bits (3195), Expect = 0.0e+00
Identity = 589/772 (76.30%), Postives = 661/772 (85.62%), Query Frame = 0

Query: 7   VLHSTALASLPLSAKKNSNLDSRDQWSSLRQ-QIELNFGSRV-SHRRQKNPFVRPFSTYK 66
           VL      S P  + K S+  SR++     Q    L FGS+  S      P  R     +
Sbjct: 7   VLSKPIRGSFPSFSPKVSSGASRNKICFPSQHSTGLKFGSQERSWDISSTPKSRVRKDER 66

Query: 67  RVHSSAISSVMTDDTSTVSSADE--SEENIGILSHDPGLQPFKDHFKYRVGRYADLLNLL 126
             HSSAIS+V+TDD ST++  +E    ENIG+L+ DP L+P+ DHF++R+ RY D   L+
Sbjct: 67  MKHSSAISAVLTDDNSTMAPLEEDVKTENIGLLNLDPTLEPYLDHFRHRMKRYVDQKMLI 126

Query: 127 EKYEGGLDEFARGYLKFGFNREEGGIVYREWAPAAQEAQIIGDFNGWDGSNHQMEKNEFG 186
           EKYEG L+EFA+GYLKFGFNRE+G IVYREWAPAAQE ++IGDFNGW+GSNH MEK++FG
Sbjct: 127 EKYEGPLEEFAQGYLKFGFNREDGCIVYREWAPAAQEDEVIGDFNGWNGSNHMMEKDQFG 186

Query: 187 TWSIKIPDLEGKPAIAHNSRVKFRFKHGNGVWIDRIPAWIKYATVDPKRFAAPYNGVYWD 246
            WSI+IPD++ KP I HNSRVKFRFKHGNGVW+DRIPAWIKYAT D  +FAAPY+GVYWD
Sbjct: 187 VWSIRIPDVDSKPVIPHNSRVKFRFKHGNGVWVDRIPAWIKYATADATKFAAPYDGVYWD 246

Query: 247 PPALERYEFKHPRPTKPEAPRVYEAHVGMSSSEPRVSSYREFADFVLPRIKENNYNTVQL 306
           PP  ERY FK+PRP KP APR+YEAHVGMSSSEPRV+SYREFAD VLPRIK NNYNTVQL
Sbjct: 247 PPPSERYHFKYPRPPKPRAPRIYEAHVGMSSSEPRVNSYREFADDVLPRIKANNYNTVQL 306

Query: 307 MAIMEHSYYASFGYHVTNFFAVSSRSGTPEDLKYLIDKAHGLGIRVLMDVVHSHASNNVT 366
           MAIMEHSYY SFGYHVTNFFAVSSR G PEDLKYLIDKAH LG++VL+DVVHSHASNNVT
Sbjct: 307 MAIMEHSYYGSFGYHVTNFFAVSSRYGNPEDLKYLIDKAHSLGLQVLVDVVHSHASNNVT 366

Query: 367 DGLNGFDVGQGTQDSYFHTGDRGYHKLWDSRIFNYANWEVLRFLLSNIRWWLEEYQFDGF 426
           DGLNGFD+GQG+Q+SYFH G+RGYHKLWDSR+FNYANWEVLRFLLSN+RWWLEEY FDGF
Sbjct: 367 DGLNGFDIGQGSQESYFHAGERGYHKLWDSRLFNYANWEVLRFLLSNLRWWLEEYNFDGF 426

Query: 427 RFDGVTSMLYHHHGINMAFTGNYNEYFSEATDVDAVVYLMLANNLIHSIFPDATVIAEDV 486
           RFDG+TSMLY HHGINM FTGNYNEYFSEATDVDAVVYLMLANNLIH IFPDATVIAEDV
Sbjct: 427 RFDGITSMLYVHHGINMGFTGNYNEYFSEATDVDAVVYLMLANNLIHKIFPDATVIAEDV 486

Query: 487 SGMPGLGRPVSEGGIGFDYRLQMAIPDKWIDYLKNKTDEEWSMGEISWNLTNRRYSEKCI 546
           SGMPGLGRPVSEGGIGFDYRL MAIPDKWIDYLKNK DE+WSM E++ +LTNRRY+EKCI
Sbjct: 487 SGMPGLGRPVSEGGIGFDYRLAMAIPDKWIDYLKNKNDEDWSMKEVTSSLTNRRYTEKCI 546

Query: 547 SYAESHDQAIVGDKTISFLLMDKEMYSGMSCLTDASPIVERGIALHKMIHFITMALGGDG 606
           +YAESHDQ+IVGDKTI+FLLMDKEMYSGMSCLTDASP+V+RGIALHKMIHF TMALGG+G
Sbjct: 547 AYAESHDQSIVGDKTIAFLLMDKEMYSGMSCLTDASPVVDRGIALHKMIHFFTMALGGEG 606

Query: 607 YLNFMGNEFGHPEWIDFPREGNGWSYDKCRRQWNLPDTDHLRYKFLNAFDRAMNLLDDKF 666
           YLNFMGNEFGHPEWIDFPREGN WSYDKCRRQWNL D++HLRYKF+NAFDRAMN LD+KF
Sbjct: 607 YLNFMGNEFGHPEWIDFPREGNNWSYDKCRRQWNLADSEHLRYKFMNAFDRAMNSLDEKF 666

Query: 667 SFLASSKQIVSWTGEEDKVIIFERGDLVFVFNFHPVNTYDGYKVGCDLPGKYRVALDSDA 726
           SFLAS KQIVS   +++KV++FERGDLVFVFNFHP NTY+GYKVGCDLPGKYRVALDSDA
Sbjct: 667 SFLASGKQIVSSMDDDNKVVVFERGDLVFVFNFHPKNTYEGYKVGCDLPGKYRVALDSDA 726

Query: 727 RDFGGLGRVGHDVDHFTSPEGIPGVPETNFNNRPNSFKVLSPARTCVVYHRV 775
            +FGG GR GHDVDHFTSPEGIPGVPETNFN R        P++ C++   V
Sbjct: 727 WEFGGHGRTGHDVDHFTSPEGIPGVPETNFNGRQ------IPSKCCLLREHV 772

BLAST of Lag0004066 vs. ExPASy Swiss-Prot
Match: Q01401 (1,4-alpha-glucan-branching enzyme, chloroplastic/amyloplastic OS=Oryza sativa subsp. japonica OX=39947 GN=SBE1 PE=1 SV=2)

HSP 1 Score: 1191.4 bits (3081), Expect = 0.0e+00
Identity = 549/736 (74.59%), Postives = 627/736 (85.19%), Query Frame = 0

Query: 81  TVSSADESEENIGILSHDPGLQPFKDHFKYRVGRYADLLNLLEKYEGGLDEFARGYLKFG 140
           T+ +  E  +++ I   DP L+ FKDHF YR+ RY D   L+EK+EGGL+EF++GYLKFG
Sbjct: 65  TMVTVVEEVDHLPIYDLDPKLEEFKDHFNYRIKRYLDQKCLIEKHEGGLEEFSKGYLKFG 124

Query: 141 FNREEGGIVYREWAPAAQEAQIIGDFNGWDGSNHQMEKNEFGTWSIKIPDLEGKPAIAHN 200
            N  +G  +YREWAPAAQEAQ+IG+FN W+G+ H+MEK++FG WSIKI  + GKPAI HN
Sbjct: 125 INTVDGATIYREWAPAAQEAQLIGEFNNWNGAKHKMEKDKFGIWSIKISHVNGKPAIPHN 184

Query: 201 SRVKFRFKHGNGVWIDRIPAWIKYATVDPKRFAAPYNGVYWDPPALERYEFKHPRPTKPE 260
           S+VKFRF+HG G W+DRIPAWI+YAT D  +F APY+GV+WDPPA ERY FKHPRP KP+
Sbjct: 185 SKVKFRFRHGGGAWVDRIPAWIRYATFDASKFGAPYDGVHWDPPACERYVFKHPRPPKPD 244

Query: 261 APRVYEAHVGMSSSEPRVSSYREFADFVLPRIKENNYNTVQLMAIMEHSYYASFGYHVTN 320
           APR+YEAHVGMS  EP VS+YREFAD VLPRI+ NNYNTVQLMAIMEHSYYASFGYHVTN
Sbjct: 245 APRIYEAHVGMSGEEPEVSTYREFADNVLPRIRANNYNTVQLMAIMEHSYYASFGYHVTN 304

Query: 321 FFAVSSRSGTPEDLKYLIDKAHGLGIRVLMDVVHSHASNNVTDGLNGFDVGQGTQDSYFH 380
           FFAVSSRSGTPEDLKYL+DKAH LG+RVLMDVVHSHASNNVTDGLNG+DVGQ T +SYFH
Sbjct: 305 FFAVSSRSGTPEDLKYLVDKAHSLGLRVLMDVVHSHASNNVTDGLNGYDVGQNTHESYFH 364

Query: 381 TGDRGYHKLWDSRIFNYANWEVLRFLLSNIRWWLEEYQFDGFRFDGVTSMLYHHHGINMA 440
           TGDRGYHKLWDSR+FNYANWEVLRFLLSN+R+W++E+ FDGFRFDGVTSMLYHHHGIN  
Sbjct: 365 TGDRGYHKLWDSRLFNYANWEVLRFLLSNLRYWMDEFMFDGFRFDGVTSMLYHHHGINKG 424

Query: 441 FTGNYNEYFSEATDVDAVVYLMLANNLIHSIFPDATVIAEDVSGMPGLGRPVSEGGIGFD 500
           FTGNY EYFS  TDVDA+VY+MLAN+L+H + P+AT++AEDVSGMP L RPV EGG+GFD
Sbjct: 425 FTGNYKEYFSLDTDVDAIVYMMLANHLMHKLLPEATIVAEDVSGMPVLCRPVDEGGVGFD 484

Query: 501 YRLQMAIPDKWIDYLKNKTDEEWSMGEISWNLTNRRYSEKCISYAESHDQAIVGDKTISF 560
           +RL MAIPD+WIDYLKNK D +WSM EI   LTNRRY+EKCI+YAESHDQ+IVGDKTI+F
Sbjct: 485 FRLAMAIPDRWIDYLKNKEDRKWSMSEIVQTLTNRRYTEKCIAYAESHDQSIVGDKTIAF 544

Query: 561 LLMDKEMYSGMSCLTDASPIVERGIALHKMIHFITMALGGDGYLNFMGNEFGHPEWIDFP 620
           LLMDKEMY+GMS L  ASP + RGIAL KMIHFITMALGGDGYLNFMGNEFGHPEWIDFP
Sbjct: 545 LLMDKEMYTGMSDLQPASPTINRGIALQKMIHFITMALGGDGYLNFMGNEFGHPEWIDFP 604

Query: 621 REGNGWSYDKCRRQWNLPDTDHLRYKFLNAFDRAMNLLDDKFSFLASSKQIVSWTGEEDK 680
           REGN WSYDKCRRQW+L DTDHLRYK++NAFD+AMN L+++FSFL+SSKQIVS   E+DK
Sbjct: 605 REGNNWSYDKCRRQWSLVDTDHLRYKYMNAFDQAMNALEEEFSFLSSSKQIVSDMNEKDK 664

Query: 681 VIIFERGDLVFVFNFHPVNTYDGYKVGCDLPGKYRVALDSDARDFGGLGRVGHDVDHFTS 740
           VI+FERGDLVFVFNFHP  TY GYKVGCDLPGKYRVALDSDA  FGG GRVGHDVDHFTS
Sbjct: 665 VIVFERGDLVFVFNFHPNKTYKGYKVGCDLPGKYRVALDSDALVFGGHGRVGHDVDHFTS 724

Query: 741 PEGIPGVPETNFNNRPNSFKVLSPARTCVVYHRVDESKEEIENGDLVGVNKPVVTEVSTR 800
           PEG+PGVPETNFNNRPNSFKVLSP RTCV Y+RVDE +EE+  G  V   K V   +   
Sbjct: 725 PEGMPGVPETNFNNRPNSFKVLSPPRTCVAYYRVDEDREELRRGGAVASGKIVTEYIDVE 784

Query: 801 LGRSEKENGDSVGANK 817
               E  +G   G+ K
Sbjct: 785 ATSGETISGGWKGSEK 800

BLAST of Lag0004066 vs. ExPASy Swiss-Prot
Match: O23647 (1,4-alpha-glucan-branching enzyme 2-1, chloroplastic/amyloplastic OS=Arabidopsis thaliana OX=3702 GN=SBE2.1 PE=1 SV=1)

HSP 1 Score: 854.7 bits (2207), Expect = 1.0e-246
Identity = 410/704 (58.24%), Postives = 510/704 (72.44%), Query Frame = 0

Query: 98  DPGLQPFKDHFKYRVGRYADLLNLLEKYEGGLDEFARGYLKFGFNREEGGIVYREWAPAA 157
           DP L   ++H  YR G+Y  L   ++K EGGL+ F+RGY  FGF R   GI YREWAP A
Sbjct: 164 DPMLNSHRNHLDYRYGQYRKLREEIDKNEGGLEAFSRGYEIFGFTRSATGITYREWAPGA 223

Query: 158 QEAQIIGDFNGWDGSNHQMEKNEFGTWSIKIP-DLEGKPAIAHNSRVKFRFKHGNGVWID 217
           + A +IGDFN W+  +  M +N+FG W I +P + +G PAI H SRVK R    +G+  D
Sbjct: 224 KAASLIGDFNNWNAKSDVMARNDFGVWEIFLPNNADGSPAIPHGSRVKIRMDTPSGI-KD 283

Query: 218 RIPAWIKYATVDPKRFAAPYNGVYWDPPALERYEFKHPRPTKPEAPRVYEAHVGMSSSEP 277
            IPAWIKY+   P     PYNGVY+DPP  ++Y FKHPRP KP + R+YE+HVGMSS+EP
Sbjct: 284 SIPAWIKYSVQPPGEI--PYNGVYYDPPEEDKYAFKHPRPKKPTSLRIYESHVGMSSTEP 343

Query: 278 RVSSYREFADFVLPRIKENNYNTVQLMAIMEHSYYASFGYHVTNFFAVSSRSGTPEDLKY 337
           ++++Y  F D VLPRIK+  YN VQ+MAI EH+YYASFGYHVTNFFA SSR GTP+DLK 
Sbjct: 344 KINTYANFRDDVLPRIKKLGYNAVQIMAIQEHAYYASFGYHVTNFFAPSSRFGTPDDLKS 403

Query: 338 LIDKAHGLGIRVLMDVVHSHASNNVTDGLNGFDVGQGTQDSYFHTGDRGYHKLWDSRIFN 397
           LIDKAH LG+ VLMD+VHSHAS N  DGL+ FD   GT   YFH+G RGYH +WDSR+FN
Sbjct: 404 LIDKAHELGLVVLMDIVHSHASKNTLDGLDMFD---GTDGQYFHSGSRGYHWMWDSRLFN 463

Query: 398 YANWEVLRFLLSNIRWWLEEYQFDGFRFDGVTSMLYHHHGINMAFTGNYNEYFSEATDVD 457
           Y +WEVLR+LLSN RWWLEEY+FDGFRFDGVTSM+Y HHG+ + FTGNYNEYF  +TDVD
Sbjct: 464 YGSWEVLRYLLSNARWWLEEYKFDGFRFDGVTSMMYTHHGLQVEFTGNYNEYFGYSTDVD 523

Query: 458 AVVYLMLANNLIHSIFPDATVIAEDVSGMPGLGRPVSEGGIGFDYRLQMAIPDKWIDYLK 517
           AVVYLML N+LIH ++P+A V+ EDVSGMP    PV +GG+GFDYRL MA+ DKWI+ LK
Sbjct: 524 AVVYLMLVNDLIHGLYPEAIVVGEDVSGMPAFCVPVEDGGVGFDYRLHMAVADKWIELLK 583

Query: 518 NKTDEEWSMGEISWNLTNRRYSEKCISYAESHDQAIVGDKTISFLLMDKEMYSGMSCLTD 577
            K DE+W +G+I++ LTNRR+ EKC+ YAESHDQA+VGDKTI+F LMDK+MY  M+    
Sbjct: 584 -KRDEDWQVGDITFTLTNRRWGEKCVVYAESHDQALVGDKTIAFWLMDKDMYDFMAVDRQ 643

Query: 578 ASPIVERGIALHKMIHFITMALGGDGYLNFMGNEFGHPEWIDFPR-----------EGNG 637
           A+P V+RGIALHKMI  ITM LGG+GYLNFMGNEFGHPEWIDFPR            GN 
Sbjct: 644 ATPRVDRGIALHKMIRLITMGLGGEGYLNFMGNEFGHPEWIDFPRTDQHLPDGRVIAGNN 703

Query: 638 WSYDKCRRQWNLPDTDHLRYKFLNAFDRAMNLLDDKFSFLASSKQIVSWTGEEDKVIIFE 697
            SYDKCRR+++L D ++LRY  L  FDRAM  L++ + F+ S  Q +S   E D+VI+FE
Sbjct: 704 GSYDKCRRRFDLGDAEYLRYHGLQEFDRAMQNLEETYGFMTSEHQYISRKDEGDRVIVFE 763

Query: 698 RGDLVFVFNFHPVNTYDGYKVGCDLPGKYRVALDSDARDFGGLGRVGHDVDHFTSPEGIP 757
           RG+L+FVFNFH  N+Y  Y++GC +PGKY++ LDSD   FGG  R+    + FTS     
Sbjct: 764 RGNLLFVFNFHWTNSYSDYRIGCSVPGKYKIVLDSDNSLFGGFNRLDDSAEFFTS----- 823

Query: 758 GVPETNFNNRPNSFKVLSPARTCVVYHRVDESKEEIENGDLVGV 790
              +   ++RP SF V +P RT VVY  VD+  ++ E   LV +
Sbjct: 824 ---DGRHDDRPCSFMVYAPCRTAVVYAAVDDDDDD-ERSSLVPI 851

BLAST of Lag0004066 vs. ExPASy Swiss-Prot
Match: Q9LZS3 (1,4-alpha-glucan-branching enzyme 2-2, chloroplastic/amyloplastic OS=Arabidopsis thaliana OX=3702 GN=SBE2.2 PE=1 SV=1)

HSP 1 Score: 851.7 bits (2199), Expect = 8.6e-246
Identity = 406/686 (59.18%), Postives = 507/686 (73.91%), Query Frame = 0

Query: 98  DPGLQPFKDHFKYRVGRYADLLNLLEKYEGGLDEFARGYLKFGFNREEGGIVYREWAPAA 157
           DP L+ + +H  YR G+Y  L   ++KYEGGL+ F+RGY K GF+R + GI YREWAP A
Sbjct: 129 DPMLRTYNNHLDYRYGQYKRLREEIDKYEGGLEAFSRGYEKLGFSRSDAGITYREWAPGA 188

Query: 158 QEAQIIGDFNGWDGSNHQMEKNEFGTWSIKIP-DLEGKPAIAHNSRVKFRFKHGNGVWID 217
           + A +IGDFN W+ +   M +NEFG W I +P + +G PAI H SRVK R    +G+  D
Sbjct: 189 KAASLIGDFNNWNSNADIMTRNEFGVWEIFLPNNTDGSPAIPHGSRVKIRMDTPSGI-KD 248

Query: 218 RIPAWIKYATVDPKRFAAPYNGVYWDPPALERYEFKHPRPTKPEAPRVYEAHVGMSSSEP 277
            IPAWIK++   P     P+NG+Y+DPP  E+Y FKHP+P +P++ R+YEAHVGMSS+EP
Sbjct: 249 SIPAWIKFSVQAPGEI--PFNGIYYDPPEEEKYVFKHPQPKRPKSLRIYEAHVGMSSTEP 308

Query: 278 RVSSYREFADFVLPRIKENNYNTVQLMAIMEHSYYASFGYHVTNFFAVSSRSGTPEDLKY 337
            V++Y  F D VLPRIK+  YN VQ+MAI EHSYYASFGYHVTNFFA SSR GTPE+LK 
Sbjct: 309 MVNTYANFRDDVLPRIKKLGYNAVQIMAIQEHSYYASFGYHVTNFFAPSSRCGTPEELKS 368

Query: 338 LIDKAHGLGIRVLMDVVHSHASNNVTDGLNGFDVGQGTQDSYFHTGDRGYHKLWDSRIFN 397
           LID+AH LG+ VLMD+VHSHAS N  DGLN FD   GT   YFH+G RGYH +WDSR+FN
Sbjct: 369 LIDRAHELGLVVLMDIVHSHASKNTLDGLNMFD---GTDAHYFHSGPRGYHWMWDSRLFN 428

Query: 398 YANWEVLRFLLSNIRWWLEEYQFDGFRFDGVTSMLYHHHGINMAFTGNYNEYFSEATDVD 457
           Y +WEVLR+LLSN RWWLEEY+FDGFRFDGVTSM+Y HHG+++ FTGNY EYF   TDVD
Sbjct: 429 YGSWEVLRYLLSNARWWLEEYKFDGFRFDGVTSMMYTHHGLSVGFTGNYTEYFGLETDVD 488

Query: 458 AVVYLMLANNLIHSIFPDATVIAEDVSGMPGLGRPVSEGGIGFDYRLQMAIPDKWIDYLK 517
           AV YLML N++IH ++P+A  + EDVSGMP    PV +GG+GFDYRL MAI DKWI+ LK
Sbjct: 489 AVNYLMLVNDMIHGLYPEAITVGEDVSGMPTFCIPVQDGGVGFDYRLHMAIADKWIEMLK 548

Query: 518 NKTDEEWSMGEISWNLTNRRYSEKCISYAESHDQAIVGDKTISFLLMDKEMYSGMSCLTD 577
            K DE+W MG+I + LTNRR+SEKCISYAESHDQA+VGDKTI+F LMDK+MY  M+    
Sbjct: 549 -KRDEDWQMGDIIYTLTNRRWSEKCISYAESHDQALVGDKTIAFWLMDKDMYDFMAVDRP 608

Query: 578 ASPIVERGIALHKMIHFITMALGGDGYLNFMGNEFGHPEWIDFPR-----------EGNG 637
           ++P+++RGIALHKMI  ITM LGG+GYLNFMGNEFGHPEWIDFPR            GN 
Sbjct: 609 STPLIDRGIALHKMIRLITMGLGGEGYLNFMGNEFGHPEWIDFPRGEQRLSDGSVIPGNN 668

Query: 638 WSYDKCRRQWNLPDTDHLRYKFLNAFDRAMNLLDDKFSFLASSKQIVSWTGEEDKVIIFE 697
           +SYDKCRR+++L D D+LRY+ L  FD+AM  L++ + F+ S  Q +S   E D+VI+FE
Sbjct: 669 FSYDKCRRRFDLGDADYLRYRGLQEFDQAMQHLEENYGFMTSEHQFISRKDEADRVIVFE 728

Query: 698 RGDLVFVFNFHPVNTYDGYKVGCDLPGKYRVALDSDARDFGGLGRVGHDVDHFTSPEGIP 757
           RGDLVFVFNFH  ++Y  Y++GC  PGKY++ LDSD   FGG  R+    ++FT  +G+ 
Sbjct: 729 RGDLVFVFNFHWTSSYFDYRIGCSKPGKYKIVLDSDDPLFGGFNRLDRKAEYFTY-DGL- 788

Query: 758 GVPETNFNNRPNSFKVLSPARTCVVY 772
                 ++ RP SF V +P RT VVY
Sbjct: 789 ------YDERPCSFMVYAPCRTAVVY 799

BLAST of Lag0004066 vs. ExPASy TrEMBL
Match: A0A6J1DI71 (1,4-alpha-glucan branching enzyme OS=Momordica charantia OX=3673 GN=LOC111020717 PE=3 SV=1)

HSP 1 Score: 1513.0 bits (3916), Expect = 0.0e+00
Identity = 725/842 (86.10%), Postives = 772/842 (91.69%), Query Frame = 0

Query: 1   MSTSPCVLHSTALASLPLSAKKNSNLDSRDQWSSLRQQIELNFGSRVSHRRQKNPFVRPF 60
           M  S CVL  T+LASL LSAKKN +  SR++W SLR+QIEL F SR+SH R  NPF+ P 
Sbjct: 1   MLASSCVLPPTSLASLSLSAKKNFSAGSRNKWPSLRRQIELIFVSRMSHHRHMNPFMHPI 60

Query: 61  STYKRVHSSAISSVMTDDTSTVSSADESEENIGILSHDPGLQPFKDHFKYRVGRYADLLN 120
           STYKR HSSAIS VMTD+  T++SADE  EN+GILSHDPGL+PFKDHFKYR+GRYA+LL+
Sbjct: 61  STYKRGHSSAISCVMTDNAPTITSADEIMENMGILSHDPGLEPFKDHFKYRIGRYANLLS 120

Query: 121 LLEKYEGGLDEFARGYLKFGFNREEGGIVYREWAPAAQEAQIIGDFNGWDGSNHQMEKNE 180
           LLEK+EGGLDEFA+GYLKFGFNREEGGIVYREWAPAAQEAQIIGDFNGWDGSNHQMEKNE
Sbjct: 121 LLEKHEGGLDEFAQGYLKFGFNREEGGIVYREWAPAAQEAQIIGDFNGWDGSNHQMEKNE 180

Query: 181 FGTWSIKIPDLEGKPAIAHNSRVKFRFKHGNGVWIDRIPAWIKYATVDPKRFAAPYNGVY 240
           FG WSIK+PD  GKPAI HNSRVKFRFKHGNGVWIDRIPAWI+YATVDP RFAAPY+GVY
Sbjct: 181 FGIWSIKLPDNGGKPAITHNSRVKFRFKHGNGVWIDRIPAWIRYATVDPTRFAAPYDGVY 240

Query: 241 WDPPALERYEFKHPRPTKPEAPRVYEAHVGMSSSEPRVSSYREFADFVLPRIKENNYNTV 300
           WDPP LERYEF+HPRP KPEAPR+YEAHVGMSSSEPRV+SYREFADF+LPRIKENNYNTV
Sbjct: 241 WDPPPLERYEFEHPRPAKPEAPRIYEAHVGMSSSEPRVNSYREFADFILPRIKENNYNTV 300

Query: 301 QLMAIMEHSYYASFGYHVTNFFAVSSRSGTPEDLKYLIDKAHGLGIRVLMDVVHSHASNN 360
           QLMAIMEHSYYASFGYHVTNFFAVSSRSGTPEDLKYLIDKAHGLG+RVLMDV+HSHASNN
Sbjct: 301 QLMAIMEHSYYASFGYHVTNFFAVSSRSGTPEDLKYLIDKAHGLGLRVLMDVIHSHASNN 360

Query: 361 VTDGLNGFDVGQGTQDSYFHTGDRGYHKLWDSRIFNYANWEVLRFLLSNIRWWLEEYQFD 420
           VTDGLNGFDVGQ +Q SYFHTGDRGYHKLWDSR+FNYANWEVLRFLLSNIRWWLEEY+FD
Sbjct: 361 VTDGLNGFDVGQSSQGSYFHTGDRGYHKLWDSRLFNYANWEVLRFLLSNIRWWLEEYKFD 420

Query: 421 GFRFDGVTSMLYHHHGINMAFTGNYNEYFSEATDVDAVVYLMLANNLIHSIFPDATVIAE 480
           GFRFDGVTSMLYHHHGINM FTGNYNEYFSEATDVDAVVYLMLANNLIHSI PDATVIAE
Sbjct: 421 GFRFDGVTSMLYHHHGINMGFTGNYNEYFSEATDVDAVVYLMLANNLIHSILPDATVIAE 480

Query: 481 DVSGMPGLGRPVSEGGIGFDYRLQMAIPDKWIDYLKNKTDEEWSMGEISWNLTNRRYSEK 540
           DVSGMPGLGRPVSEGGIGFDYRLQMAIPDKWIDYLKNKTDEEWSMGEISWNLTNRRYSEK
Sbjct: 481 DVSGMPGLGRPVSEGGIGFDYRLQMAIPDKWIDYLKNKTDEEWSMGEISWNLTNRRYSEK 540

Query: 541 CISYAESHDQAIVGDKTISFLLMDKEMYSGMSCLTDASPIVERGIALHKMIHFITMALGG 600
           CISYAESHDQAIVGDKT++FLLMDKEMYSGMSCLT+ASPIVERGIALHKMIHFITMALGG
Sbjct: 541 CISYAESHDQAIVGDKTVAFLLMDKEMYSGMSCLTEASPIVERGIALHKMIHFITMALGG 600

Query: 601 DGYLNFMGNEFGHPEWIDFPREGNGWSYDKCRRQWNLPDTDHLRYKFLNAFDRAMNLLDD 660
           +GYLNFMGNEFGHPEWIDFPREGNGWSYDKCRRQWNLPDTDHLRYKFLNAFDRAMNLLDD
Sbjct: 601 EGYLNFMGNEFGHPEWIDFPREGNGWSYDKCRRQWNLPDTDHLRYKFLNAFDRAMNLLDD 660

Query: 661 KFSFLASSKQIVSWTGEEDKVIIFERGDLVFVFNFHPVNTYDGYKVGCDLPGKYRVALDS 720
           KFSFLASSKQI+S T EEDKVI+FERGDLVFVFNFHPVNTYDGYKVGCDLPGKYRVALDS
Sbjct: 661 KFSFLASSKQILSCTSEEDKVIVFERGDLVFVFNFHPVNTYDGYKVGCDLPGKYRVALDS 720

Query: 721 DARDFGGLGRVGHDVDHFTSPEGIPGVPETNFNNRPNSFKVLSPARTCVVYHRVDESKEE 780
           DA DFGG GR+GHD DHFTSPEGIPGVPETNFNNRPNSFKVLSPARTCVVY+RVDESKE+
Sbjct: 721 DAWDFGGHGRIGHDADHFTSPEGIPGVPETNFNNRPNSFKVLSPARTCVVYYRVDESKEK 780

Query: 781 IENGDLVGVNKPVVTEVSTRLGRSE------KENGD-SVGANKPIAIDVSTQLGHCEKEN 836
            ENGD VG NKP+ T+VS R    E       E GD +VG  +   ++    +   + E+
Sbjct: 781 -ENGDSVGTNKPLTTDVSARHTEEEDEELTHSEEGDIAVGETRTTNVE-DDNIDTSKSED 840

BLAST of Lag0004066 vs. ExPASy TrEMBL
Match: E5GBZ3 (1,4-alpha-glucan branching enzyme OS=Cucumis melo subsp. melo OX=412675 PE=3 SV=1)

HSP 1 Score: 1508.4 bits (3904), Expect = 0.0e+00
Identity = 733/847 (86.54%), Postives = 771/847 (91.03%), Query Frame = 0

Query: 1   MSTSPCVLHSTALASLPLSAKKNSNLDSRDQWSSLRQQIELNFGSRVSHRRQKNPFVRPF 60
           M  SPCVL STA+ASL LSAKK    DSR+QW SLRQQ ELNF SR+SH RQ NPF+ P+
Sbjct: 1   MLLSPCVLPSTAIASLSLSAKKIFISDSRNQWPSLRQQTELNFWSRMSHHRQMNPFIFPY 60

Query: 61  STYKRVHSSAISSVMTDDTSTVSSADESEENIGILSHDPGLQPFKDHFKYRVGRYADLLN 120
           STYKRVHS AISSVMTDDTSTVSS DES ENIGILSHDPGL+PFKDHFKYRVGRY DLLN
Sbjct: 61  STYKRVHSPAISSVMTDDTSTVSSNDESMENIGILSHDPGLKPFKDHFKYRVGRYTDLLN 120

Query: 121 LLEKYEGGLDEFARGYLKFGFNREEGGIVYREWAPAAQEAQIIGDFNGWDGSNHQMEKNE 180
           LL+K+EGGLDEFA+GYLKFGFNREE GIVYREWAPAAQEAQI+GDFNGW+G+NH MEKNE
Sbjct: 121 LLDKHEGGLDEFAQGYLKFGFNREEDGIVYREWAPAAQEAQIVGDFNGWNGTNHCMEKNE 180

Query: 181 FGTWSIKIPDLEGKPAIAHNSRVKFRFKHGNGVWIDRIPAWIKYATVDPKRFAAPYNGVY 240
           FG WSIKI DL GKPAI+HNSRVKFRFKHGNGVWIDRIPAWIKYATVDP +FAAPY+GVY
Sbjct: 181 FGIWSIKIYDLGGKPAISHNSRVKFRFKHGNGVWIDRIPAWIKYATVDPTKFAAPYDGVY 240

Query: 241 WDPPALERYEFKHPRPTKPEAPRVYEAHVGMSSSEPRVSSYREFADFVLPRIKENNYNTV 300
           WDPP LERYEFKHPRP KP  PRVYEAHVGMSSSEPRVSSYREFADFVLPRIKENNYNTV
Sbjct: 241 WDPPPLERYEFKHPRPAKPNGPRVYEAHVGMSSSEPRVSSYREFADFVLPRIKENNYNTV 300

Query: 301 QLMAIMEHSYYASFGYHVTNFFAVSSRSGTPEDLKYLIDKAHGLGIRVLMDVVHSHASNN 360
           QLMAIMEHSYYASFGYHVTNFFAVSSRSGTPEDLKYLIDKAHGLG+RVLMDVVHSHASNN
Sbjct: 301 QLMAIMEHSYYASFGYHVTNFFAVSSRSGTPEDLKYLIDKAHGLGLRVLMDVVHSHASNN 360

Query: 361 VTDGLNGFDVGQGTQDSYFHTGDRGYHKLWDSRIFNYANWEVLRFLLSNIRWWLEEYQFD 420
           VTDGLNGFDVGQ TQDSYFHTGDRGYHKLWDSR+FNYANWEVLRFLLSNIRWWLEEYQFD
Sbjct: 361 VTDGLNGFDVGQSTQDSYFHTGDRGYHKLWDSRLFNYANWEVLRFLLSNIRWWLEEYQFD 420

Query: 421 GFRFDGVTSMLYHHHGINMAFTGNYNEYFSEATDVDAVVYLMLANNLIHSIFPDATVIAE 480
           GFRFDGVTSMLYHHHGI+MAFTGNYNEYFSEATDVDAVVYLMLANNL HSI PDATVIAE
Sbjct: 421 GFRFDGVTSMLYHHHGISMAFTGNYNEYFSEATDVDAVVYLMLANNLTHSILPDATVIAE 480

Query: 481 DVSGMPGLGRPVSEGGIGFDYRLQMAIPDKWIDYLKNKTDEEWSMGEISWNLTNRRYSEK 540
           DVSGMPGLGRPV EGGIGFDYRLQMAIPDKWIDYLKNK+DEEWSMGEISWNLTNRRYSEK
Sbjct: 481 DVSGMPGLGRPVFEGGIGFDYRLQMAIPDKWIDYLKNKSDEEWSMGEISWNLTNRRYSEK 540

Query: 541 CISYAESHDQAIVGDKTISFLLMDKEMYSGMSCLTDASPIVERGIALHKMIHFITMALGG 600
           CISYAESHDQ+IVGDKTI+FLLMDKEMYSGMSCL +ASP+VERGIALHKMIHFITMALGG
Sbjct: 541 CISYAESHDQSIVGDKTIAFLLMDKEMYSGMSCLENASPVVERGIALHKMIHFITMALGG 600

Query: 601 DGYLNFMGNEFGHPEWIDFPREGNGWSYDKCRRQWNLPDTDHLRYKFLNAFDRAMNLLDD 660
           +GYLNFMGNEFGHPEWIDFPREGNGWSYDKCRRQWNL DTDHLRYKFLNAFDRAMN LD+
Sbjct: 601 EGYLNFMGNEFGHPEWIDFPREGNGWSYDKCRRQWNLADTDHLRYKFLNAFDRAMNALDE 660

Query: 661 KFSFLASSKQIVSWTGEEDKVIIFERGDLVFVFNFHPVNTYDGYKVGCDLPGKYRVALDS 720
           KFSFLASSKQIVSWTGEEDKVI+FERGDLVFVFNFHP+NTYDGYKVGCDLPGKYRVALDS
Sbjct: 661 KFSFLASSKQIVSWTGEEDKVIVFERGDLVFVFNFHPINTYDGYKVGCDLPGKYRVALDS 720

Query: 721 DARDFGGLGRVGHDVDHFTSPEGIPGVPETNFNNRPNSFKVLSPARTCVVYHRVDESKEE 780
           DA DFGG GRVGHD+DHFTSPEGIPGVPETNFNNRPNSFKVLSPARTCVVY++VDESKE+
Sbjct: 721 DAGDFGGYGRVGHDIDHFTSPEGIPGVPETNFNNRPNSFKVLSPARTCVVYYKVDESKEK 780

Query: 781 IENGDLVG-----VNKPVVTEVSTRLGRSEKENGDSVG-ANKPIAIDVSTQLGHCEKENG 840
            E  DLV      V+   V E S  L   E+EN  +VG  +K    D+ T      K   
Sbjct: 781 -EKDDLVASVHEDVSARHVEEDSEGLAGREEENDIAVGKISKTEDDDIDT-----SKTEE 840

Query: 841 DSVGANE 842
           D V +N+
Sbjct: 841 DDVDSNK 841

BLAST of Lag0004066 vs. ExPASy TrEMBL
Match: A0A1S3CMX9 (1,4-alpha-glucan branching enzyme OS=Cucumis melo OX=3656 GN=LOC103502624 PE=3 SV=1)

HSP 1 Score: 1508.4 bits (3904), Expect = 0.0e+00
Identity = 733/847 (86.54%), Postives = 771/847 (91.03%), Query Frame = 0

Query: 1   MSTSPCVLHSTALASLPLSAKKNSNLDSRDQWSSLRQQIELNFGSRVSHRRQKNPFVRPF 60
           M  SPCVL STA+ASL LSAKK    DSR+QW SLRQQ ELNF SR+SH RQ NPF+ P+
Sbjct: 1   MLLSPCVLPSTAIASLSLSAKKIFISDSRNQWPSLRQQTELNFWSRMSHHRQMNPFIFPY 60

Query: 61  STYKRVHSSAISSVMTDDTSTVSSADESEENIGILSHDPGLQPFKDHFKYRVGRYADLLN 120
           STYKRVHS AISSVMTDDTSTVSS DES ENIGILSHDPGL+PFKDHFKYRVGRY DLLN
Sbjct: 61  STYKRVHSPAISSVMTDDTSTVSSNDESMENIGILSHDPGLKPFKDHFKYRVGRYTDLLN 120

Query: 121 LLEKYEGGLDEFARGYLKFGFNREEGGIVYREWAPAAQEAQIIGDFNGWDGSNHQMEKNE 180
           LL+K+EGGLDEFA+GYLKFGFNREE GIVYREWAPAAQEAQI+GDFNGW+G+NH MEKNE
Sbjct: 121 LLDKHEGGLDEFAQGYLKFGFNREEDGIVYREWAPAAQEAQIVGDFNGWNGTNHCMEKNE 180

Query: 181 FGTWSIKIPDLEGKPAIAHNSRVKFRFKHGNGVWIDRIPAWIKYATVDPKRFAAPYNGVY 240
           FG WSIKI DL GKPAI+HNSRVKFRFKHGNGVWIDRIPAWIKYATVDP +FAAPY+GVY
Sbjct: 181 FGIWSIKIYDLGGKPAISHNSRVKFRFKHGNGVWIDRIPAWIKYATVDPTKFAAPYDGVY 240

Query: 241 WDPPALERYEFKHPRPTKPEAPRVYEAHVGMSSSEPRVSSYREFADFVLPRIKENNYNTV 300
           WDPP LERYEFKHPRP KP  PRVYEAHVGMSSSEPRVSSYREFADFVLPRIKENNYNTV
Sbjct: 241 WDPPPLERYEFKHPRPAKPNGPRVYEAHVGMSSSEPRVSSYREFADFVLPRIKENNYNTV 300

Query: 301 QLMAIMEHSYYASFGYHVTNFFAVSSRSGTPEDLKYLIDKAHGLGIRVLMDVVHSHASNN 360
           QLMAIMEHSYYASFGYHVTNFFAVSSRSGTPEDLKYLIDKAHGLG+RVLMDVVHSHASNN
Sbjct: 301 QLMAIMEHSYYASFGYHVTNFFAVSSRSGTPEDLKYLIDKAHGLGLRVLMDVVHSHASNN 360

Query: 361 VTDGLNGFDVGQGTQDSYFHTGDRGYHKLWDSRIFNYANWEVLRFLLSNIRWWLEEYQFD 420
           VTDGLNGFDVGQ TQDSYFHTGDRGYHKLWDSR+FNYANWEVLRFLLSNIRWWLEEYQFD
Sbjct: 361 VTDGLNGFDVGQSTQDSYFHTGDRGYHKLWDSRLFNYANWEVLRFLLSNIRWWLEEYQFD 420

Query: 421 GFRFDGVTSMLYHHHGINMAFTGNYNEYFSEATDVDAVVYLMLANNLIHSIFPDATVIAE 480
           GFRFDGVTSMLYHHHGI+MAFTGNYNEYFSEATDVDAVVYLMLANNL HSI PDATVIAE
Sbjct: 421 GFRFDGVTSMLYHHHGISMAFTGNYNEYFSEATDVDAVVYLMLANNLTHSILPDATVIAE 480

Query: 481 DVSGMPGLGRPVSEGGIGFDYRLQMAIPDKWIDYLKNKTDEEWSMGEISWNLTNRRYSEK 540
           DVSGMPGLGRPV EGGIGFDYRLQMAIPDKWIDYLKNK+DEEWSMGEISWNLTNRRYSEK
Sbjct: 481 DVSGMPGLGRPVFEGGIGFDYRLQMAIPDKWIDYLKNKSDEEWSMGEISWNLTNRRYSEK 540

Query: 541 CISYAESHDQAIVGDKTISFLLMDKEMYSGMSCLTDASPIVERGIALHKMIHFITMALGG 600
           CISYAESHDQ+IVGDKTI+FLLMDKEMYSGMSCL +ASP+VERGIALHKMIHFITMALGG
Sbjct: 541 CISYAESHDQSIVGDKTIAFLLMDKEMYSGMSCLENASPVVERGIALHKMIHFITMALGG 600

Query: 601 DGYLNFMGNEFGHPEWIDFPREGNGWSYDKCRRQWNLPDTDHLRYKFLNAFDRAMNLLDD 660
           +GYLNFMGNEFGHPEWIDFPREGNGWSYDKCRRQWNL DTDHLRYKFLNAFDRAMN LD+
Sbjct: 601 EGYLNFMGNEFGHPEWIDFPREGNGWSYDKCRRQWNLADTDHLRYKFLNAFDRAMNALDE 660

Query: 661 KFSFLASSKQIVSWTGEEDKVIIFERGDLVFVFNFHPVNTYDGYKVGCDLPGKYRVALDS 720
           KFSFLASSKQIVSWTGEEDKVI+FERGDLVFVFNFHP+NTYDGYKVGCDLPGKYRVALDS
Sbjct: 661 KFSFLASSKQIVSWTGEEDKVIVFERGDLVFVFNFHPINTYDGYKVGCDLPGKYRVALDS 720

Query: 721 DARDFGGLGRVGHDVDHFTSPEGIPGVPETNFNNRPNSFKVLSPARTCVVYHRVDESKEE 780
           DA DFGG GRVGHD+DHFTSPEGIPGVPETNFNNRPNSFKVLSPARTCVVY++VDESKE+
Sbjct: 721 DAGDFGGYGRVGHDIDHFTSPEGIPGVPETNFNNRPNSFKVLSPARTCVVYYKVDESKEK 780

Query: 781 IENGDLVG-----VNKPVVTEVSTRLGRSEKENGDSVG-ANKPIAIDVSTQLGHCEKENG 840
            E  DLV      V+   V E S  L   E+EN  +VG  +K    D+ T      K   
Sbjct: 781 -EKDDLVASVHEDVSARHVEEDSEGLAGREEENDIAVGKISKTEDDDIDT-----SKTEE 840

Query: 841 DSVGANE 842
           D V +N+
Sbjct: 841 DDVDSNK 841

BLAST of Lag0004066 vs. ExPASy TrEMBL
Match: A0A0A0KGQ9 (1,4-alpha-glucan branching enzyme OS=Cucumis sativus OX=3659 GN=Csa_6G357030 PE=3 SV=1)

HSP 1 Score: 1506.5 bits (3899), Expect = 0.0e+00
Identity = 731/847 (86.30%), Postives = 772/847 (91.15%), Query Frame = 0

Query: 1   MSTSPCVLHSTALASLPLSAKKNSNLDSRDQWSSLRQQIELNFGSRVSHRRQKNPFVRPF 60
           M  S CVL STA+ASL LSAKK    DSR+QW SLRQQ ELNF SR+SH RQ NPFV P+
Sbjct: 1   MLLSSCVLPSTAVASLSLSAKKIIISDSRNQWPSLRQQTELNFWSRMSHHRQMNPFVFPY 60

Query: 61  STYKRVHSSAISSVMTDDTSTVSSADESEENIGILSHDPGLQPFKDHFKYRVGRYADLLN 120
           STYKRVHS AISSVMT+DTSTVSS DES ENIGILSHDPGL+PFKDHFKYRVGRY DLLN
Sbjct: 61  STYKRVHSPAISSVMTEDTSTVSSTDESMENIGILSHDPGLKPFKDHFKYRVGRYTDLLN 120

Query: 121 LLEKYEGGLDEFARGYLKFGFNREEGGIVYREWAPAAQEAQIIGDFNGWDGSNHQMEKNE 180
           LL+K+EGGLDEFARGYLKFGFNREE GIVYREWAPAAQEAQI+GDFNGWDG+NH MEKNE
Sbjct: 121 LLDKHEGGLDEFARGYLKFGFNREEDGIVYREWAPAAQEAQIVGDFNGWDGTNHCMEKNE 180

Query: 181 FGTWSIKIPDLEGKPAIAHNSRVKFRFKHGNGVWIDRIPAWIKYATVDPKRFAAPYNGVY 240
           FG WSIK+ DL GKPAI+HNSRVKFRFKHGNGVWIDRIPAWIKYATVDP +FAAPY+GVY
Sbjct: 181 FGIWSIKVYDLGGKPAISHNSRVKFRFKHGNGVWIDRIPAWIKYATVDPTKFAAPYDGVY 240

Query: 241 WDPPALERYEFKHPRPTKPEAPRVYEAHVGMSSSEPRVSSYREFADFVLPRIKENNYNTV 300
           WDPP LERYEFKHPRP KP APRVYEAHVGMSSSEPRV+SYREFADFVLPRIKENNYNTV
Sbjct: 241 WDPPPLERYEFKHPRPAKPNAPRVYEAHVGMSSSEPRVNSYREFADFVLPRIKENNYNTV 300

Query: 301 QLMAIMEHSYYASFGYHVTNFFAVSSRSGTPEDLKYLIDKAHGLGIRVLMDVVHSHASNN 360
           QLMAIMEHSYYASFGYH+TNFFAVSSRSGTPEDLKYLIDKAHGLG+RVLMDVVHSHASNN
Sbjct: 301 QLMAIMEHSYYASFGYHITNFFAVSSRSGTPEDLKYLIDKAHGLGLRVLMDVVHSHASNN 360

Query: 361 VTDGLNGFDVGQGTQDSYFHTGDRGYHKLWDSRIFNYANWEVLRFLLSNIRWWLEEYQFD 420
           VTDGLNGFDVGQ +QDSYFHTGDRGYHKLWDSR+FNYANWEVLRFLLSNIRWWLEEYQFD
Sbjct: 361 VTDGLNGFDVGQSSQDSYFHTGDRGYHKLWDSRLFNYANWEVLRFLLSNIRWWLEEYQFD 420

Query: 421 GFRFDGVTSMLYHHHGINMAFTGNYNEYFSEATDVDAVVYLMLANNLIHSIFPDATVIAE 480
           GFRFDGVTSMLYHHHGINM F+GNYNEYFSEATDVDAVVYLMLANNL HSI PDATVIAE
Sbjct: 421 GFRFDGVTSMLYHHHGINMGFSGNYNEYFSEATDVDAVVYLMLANNLTHSILPDATVIAE 480

Query: 481 DVSGMPGLGRPVSEGGIGFDYRLQMAIPDKWIDYLKNKTDEEWSMGEISWNLTNRRYSEK 540
           DVSGMPGLGRPV EGGIGFDYRLQMAIPDKWIDYLKNK+DEEWSMGEISWNLTNRRYSEK
Sbjct: 481 DVSGMPGLGRPVFEGGIGFDYRLQMAIPDKWIDYLKNKSDEEWSMGEISWNLTNRRYSEK 540

Query: 541 CISYAESHDQAIVGDKTISFLLMDKEMYSGMSCLTDASPIVERGIALHKMIHFITMALGG 600
           CISYAESHDQ+IVGDKTI+FLLMDKEMYSGMSCL +ASP+VERGIALHKMIHFITMALGG
Sbjct: 541 CISYAESHDQSIVGDKTIAFLLMDKEMYSGMSCLENASPVVERGIALHKMIHFITMALGG 600

Query: 601 DGYLNFMGNEFGHPEWIDFPREGNGWSYDKCRRQWNLPDTDHLRYKFLNAFDRAMNLLDD 660
           +GYLNFMGNEFGHPEWIDFPREGNGWSYDKCRRQWNLPDTDHLRYKFLNAFD AMN LD+
Sbjct: 601 EGYLNFMGNEFGHPEWIDFPREGNGWSYDKCRRQWNLPDTDHLRYKFLNAFDSAMNALDE 660

Query: 661 KFSFLASSKQIVSWTGEEDKVIIFERGDLVFVFNFHPVNTYDGYKVGCDLPGKYRVALDS 720
           KFSFLASSKQIVSWTGEEDKVI+FERGDLVFVFNFHPVNTYDGYKVGCDLPGKYRVALDS
Sbjct: 661 KFSFLASSKQIVSWTGEEDKVIVFERGDLVFVFNFHPVNTYDGYKVGCDLPGKYRVALDS 720

Query: 721 DARDFGGLGRVGHDVDHFTSPEGIPGVPETNFNNRPNSFKVLSPARTCVVYHRVDESKEE 780
           DA DFGG GRVGHD+DHFTSPEGIPGVPETNFNNRPNSFK+LSPARTCVVY++VDESKE+
Sbjct: 721 DASDFGGHGRVGHDIDHFTSPEGIPGVPETNFNNRPNSFKILSPARTCVVYYKVDESKEK 780

Query: 781 IENGDLVG-VNKPV----VTEVSTRLGRSEKENGDSVG-ANKPIAIDVSTQLGHCEKENG 840
            E  DLVG  N+ V    V E S  L   ++EN  +VG  +K    D+ T      K   
Sbjct: 781 -EKDDLVGSANEDVFARHVEEDSEGLAGCKEENDIAVGEISKTEDDDIDT-----SKPED 840

Query: 841 DSVGANE 842
           D V +N+
Sbjct: 841 DDVDSNK 841

BLAST of Lag0004066 vs. ExPASy TrEMBL
Match: A0A6J1KG03 (1,4-alpha-glucan branching enzyme OS=Cucurbita maxima OX=3661 GN=LOC111493933 PE=3 SV=1)

HSP 1 Score: 1488.8 bits (3853), Expect = 0.0e+00
Identity = 716/837 (85.54%), Postives = 764/837 (91.28%), Query Frame = 0

Query: 1   MSTSPCVLHSTALASLPLSAKKNSNLDSRDQWSSLRQQIELNFGSRVSHRRQKNPFVRPF 60
           M  SPCVL ST LASL LSAKKN   DSR+Q SSLRQQI LNFGSR+SH RQ +PF+ PF
Sbjct: 1   MLASPCVLPSTVLASLSLSAKKNFTSDSRNQCSSLRQQIVLNFGSRMSHHRQMHPFMDPF 60

Query: 61  STYKRVHSSA-ISSVMTDDTSTVSSADESEENIGILSHDPGLQPFKDHFKYRVGRYADLL 120
           STYKRV+  A ISSVMTDDTST SSADE+ ++IGIL HDP L+PFKDHFKYR+GRY+D+L
Sbjct: 61  STYKRVYRPAIISSVMTDDTSTASSADENMKDIGILRHDPSLEPFKDHFKYRIGRYSDVL 120

Query: 121 NLLEKYEGGLDEFARGYLKFGFNREEGGIVYREWAPAAQEAQIIGDFNGWDGSNHQMEKN 180
           NL +K+EGGLDEFARGYLKFGFNRE  GIVYREWAP AQEAQIIGDFNGWDGSNH+MEKN
Sbjct: 121 NLFDKHEGGLDEFARGYLKFGFNREGDGIVYREWAPVAQEAQIIGDFNGWDGSNHRMEKN 180

Query: 181 EFGTWSIKIPDLEGKPAIAHNSRVKFRFKHGNGVWIDRIPAWIKYATVDPKRFAAPYNGV 240
           EFG WSI IPD++GKPAIAHNSRVKFRFKHGNG WIDRIPAWIKYATVDP RF APY+GV
Sbjct: 181 EFGIWSINIPDIDGKPAIAHNSRVKFRFKHGNGAWIDRIPAWIKYATVDPTRFGAPYDGV 240

Query: 241 YWDPPALERYEFKHPRPTKPEAPRVYEAHVGMSSSEPRVSSYREFADFVLPRIKENNYNT 300
           YW+PP LERYEFKHPRP KP+APR+YEAHVGMSSSEPRV+SYREFADFVLPRIKENNYNT
Sbjct: 241 YWEPPPLERYEFKHPRPAKPKAPRIYEAHVGMSSSEPRVNSYREFADFVLPRIKENNYNT 300

Query: 301 VQLMAIMEHSYYASFGYHVTNFFAVSSRSGTPEDLKYLIDKAHGLGIRVLMDVVHSHASN 360
           VQLMAIMEHSYYASFGYHVTNFFA+SSRSGTPEDLKYLIDKAHGLG+RVLMD+VHSHASN
Sbjct: 301 VQLMAIMEHSYYASFGYHVTNFFAMSSRSGTPEDLKYLIDKAHGLGLRVLMDIVHSHASN 360

Query: 361 NVTDGLNGFDVGQGTQDSYFHTGDRGYHKLWDSRIFNYANWEVLRFLLSNIRWWLEEYQF 420
           NVTDGLNGFDVGQ TQDSYFHTGDRGYHKLWDSR+FNYANWEVLRFLLSNIRWWLEEYQF
Sbjct: 361 NVTDGLNGFDVGQSTQDSYFHTGDRGYHKLWDSRLFNYANWEVLRFLLSNIRWWLEEYQF 420

Query: 421 DGFRFDGVTSMLYHHHGINMAFTGNYNEYFSEATDVDAVVYLMLANNLIHSIFPDATVIA 480
           DGFRFDGVTSMLYHHHGIN AFTGNYNEYFSEATDVDAVVYLMLANNLIHSI P+ATVIA
Sbjct: 421 DGFRFDGVTSMLYHHHGINTAFTGNYNEYFSEATDVDAVVYLMLANNLIHSILPNATVIA 480

Query: 481 EDVSGMPGLGRPVSEGGIGFDYRLQMAIPDKWIDYLKNKTDEEWSMGEISWNLTNRRYSE 540
           EDVSGMPGLG PVSEGGIGFDYRLQMAIPDKWIDY+KNK DEEWSMGEISWNLTNRRYSE
Sbjct: 481 EDVSGMPGLGIPVSEGGIGFDYRLQMAIPDKWIDYVKNKNDEEWSMGEISWNLTNRRYSE 540

Query: 541 KCISYAESHDQAIVGDKTISFLLMDKEMYSGMSCLTDASPIVERGIALHKMIHFITMALG 600
           KCISYAESHDQ+IVGDKTI+FLLMDKEMYSGMSCLT+ASP+VERGIALHKMIHFITMALG
Sbjct: 541 KCISYAESHDQSIVGDKTIAFLLMDKEMYSGMSCLTEASPVVERGIALHKMIHFITMALG 600

Query: 601 GDGYLNFMGNEFGHPEWIDFPREGNGWSYDKCRRQWNLPDTDHLRYKFLNAFDRAMNLLD 660
           G+GYLNFMGNEFGHPEWIDFPREGNGWSYDKCRRQWNLPDTDHLRYKFLNAFDRAMN LD
Sbjct: 601 GEGYLNFMGNEFGHPEWIDFPREGNGWSYDKCRRQWNLPDTDHLRYKFLNAFDRAMNSLD 660

Query: 661 DKFSFLASSKQIVSWTGEEDKVIIFERGDLVFVFNFHPVNTYDGYKVGCDLPGKYRVALD 720
           DKFSFLASSKQIVS T EE+KVI+FERGDLVFVFNFHP+NTYDGYKVGCD+PGKYRVALD
Sbjct: 661 DKFSFLASSKQIVSCTSEEEKVIVFERGDLVFVFNFHPINTYDGYKVGCDIPGKYRVALD 720

Query: 721 SDARDFGGLGRVGHDVDHFTSPEGIPGVPETNFNNRPNSFKVLSPARTCVVYHRVDESKE 780
           SDA +FGG GRVGHD+DHFTSPEGIPGVPETNFNNRPNSFKVLSPA+TCVVY+RVDESKE
Sbjct: 721 SDASEFGGRGRVGHDIDHFTSPEGIPGVPETNFNNRPNSFKVLSPAQTCVVYYRVDESKE 780

Query: 781 EIENGDLVGVNKPVVTEV---STRLGRSEKENG-DSVGANK----PIAIDVSTQLGH 829
           + ENG  VG ++   T+V     + G   K  G D V  +K    P+  D   Q GH
Sbjct: 781 K-ENGVSVGASEAHATDVVLARDKEGEISKIKGDDDVHTSKIEDFPVREDQVEQQGH 836

BLAST of Lag0004066 vs. TAIR 10
Match: AT2G36390.1 (starch branching enzyme 2.1 )

HSP 1 Score: 854.7 bits (2207), Expect = 7.2e-248
Identity = 410/704 (58.24%), Postives = 510/704 (72.44%), Query Frame = 0

Query: 98  DPGLQPFKDHFKYRVGRYADLLNLLEKYEGGLDEFARGYLKFGFNREEGGIVYREWAPAA 157
           DP L   ++H  YR G+Y  L   ++K EGGL+ F+RGY  FGF R   GI YREWAP A
Sbjct: 164 DPMLNSHRNHLDYRYGQYRKLREEIDKNEGGLEAFSRGYEIFGFTRSATGITYREWAPGA 223

Query: 158 QEAQIIGDFNGWDGSNHQMEKNEFGTWSIKIP-DLEGKPAIAHNSRVKFRFKHGNGVWID 217
           + A +IGDFN W+  +  M +N+FG W I +P + +G PAI H SRVK R    +G+  D
Sbjct: 224 KAASLIGDFNNWNAKSDVMARNDFGVWEIFLPNNADGSPAIPHGSRVKIRMDTPSGI-KD 283

Query: 218 RIPAWIKYATVDPKRFAAPYNGVYWDPPALERYEFKHPRPTKPEAPRVYEAHVGMSSSEP 277
            IPAWIKY+   P     PYNGVY+DPP  ++Y FKHPRP KP + R+YE+HVGMSS+EP
Sbjct: 284 SIPAWIKYSVQPPGEI--PYNGVYYDPPEEDKYAFKHPRPKKPTSLRIYESHVGMSSTEP 343

Query: 278 RVSSYREFADFVLPRIKENNYNTVQLMAIMEHSYYASFGYHVTNFFAVSSRSGTPEDLKY 337
           ++++Y  F D VLPRIK+  YN VQ+MAI EH+YYASFGYHVTNFFA SSR GTP+DLK 
Sbjct: 344 KINTYANFRDDVLPRIKKLGYNAVQIMAIQEHAYYASFGYHVTNFFAPSSRFGTPDDLKS 403

Query: 338 LIDKAHGLGIRVLMDVVHSHASNNVTDGLNGFDVGQGTQDSYFHTGDRGYHKLWDSRIFN 397
           LIDKAH LG+ VLMD+VHSHAS N  DGL+ FD   GT   YFH+G RGYH +WDSR+FN
Sbjct: 404 LIDKAHELGLVVLMDIVHSHASKNTLDGLDMFD---GTDGQYFHSGSRGYHWMWDSRLFN 463

Query: 398 YANWEVLRFLLSNIRWWLEEYQFDGFRFDGVTSMLYHHHGINMAFTGNYNEYFSEATDVD 457
           Y +WEVLR+LLSN RWWLEEY+FDGFRFDGVTSM+Y HHG+ + FTGNYNEYF  +TDVD
Sbjct: 464 YGSWEVLRYLLSNARWWLEEYKFDGFRFDGVTSMMYTHHGLQVEFTGNYNEYFGYSTDVD 523

Query: 458 AVVYLMLANNLIHSIFPDATVIAEDVSGMPGLGRPVSEGGIGFDYRLQMAIPDKWIDYLK 517
           AVVYLML N+LIH ++P+A V+ EDVSGMP    PV +GG+GFDYRL MA+ DKWI+ LK
Sbjct: 524 AVVYLMLVNDLIHGLYPEAIVVGEDVSGMPAFCVPVEDGGVGFDYRLHMAVADKWIELLK 583

Query: 518 NKTDEEWSMGEISWNLTNRRYSEKCISYAESHDQAIVGDKTISFLLMDKEMYSGMSCLTD 577
            K DE+W +G+I++ LTNRR+ EKC+ YAESHDQA+VGDKTI+F LMDK+MY  M+    
Sbjct: 584 -KRDEDWQVGDITFTLTNRRWGEKCVVYAESHDQALVGDKTIAFWLMDKDMYDFMAVDRQ 643

Query: 578 ASPIVERGIALHKMIHFITMALGGDGYLNFMGNEFGHPEWIDFPR-----------EGNG 637
           A+P V+RGIALHKMI  ITM LGG+GYLNFMGNEFGHPEWIDFPR            GN 
Sbjct: 644 ATPRVDRGIALHKMIRLITMGLGGEGYLNFMGNEFGHPEWIDFPRTDQHLPDGRVIAGNN 703

Query: 638 WSYDKCRRQWNLPDTDHLRYKFLNAFDRAMNLLDDKFSFLASSKQIVSWTGEEDKVIIFE 697
            SYDKCRR+++L D ++LRY  L  FDRAM  L++ + F+ S  Q +S   E D+VI+FE
Sbjct: 704 GSYDKCRRRFDLGDAEYLRYHGLQEFDRAMQNLEETYGFMTSEHQYISRKDEGDRVIVFE 763

Query: 698 RGDLVFVFNFHPVNTYDGYKVGCDLPGKYRVALDSDARDFGGLGRVGHDVDHFTSPEGIP 757
           RG+L+FVFNFH  N+Y  Y++GC +PGKY++ LDSD   FGG  R+    + FTS     
Sbjct: 764 RGNLLFVFNFHWTNSYSDYRIGCSVPGKYKIVLDSDNSLFGGFNRLDDSAEFFTS----- 823

Query: 758 GVPETNFNNRPNSFKVLSPARTCVVYHRVDESKEEIENGDLVGV 790
              +   ++RP SF V +P RT VVY  VD+  ++ E   LV +
Sbjct: 824 ---DGRHDDRPCSFMVYAPCRTAVVYAAVDDDDDD-ERSSLVPI 851

BLAST of Lag0004066 vs. TAIR 10
Match: AT5G03650.1 (starch branching enzyme 2.2 )

HSP 1 Score: 851.7 bits (2199), Expect = 6.1e-247
Identity = 406/686 (59.18%), Postives = 507/686 (73.91%), Query Frame = 0

Query: 98  DPGLQPFKDHFKYRVGRYADLLNLLEKYEGGLDEFARGYLKFGFNREEGGIVYREWAPAA 157
           DP L+ + +H  YR G+Y  L   ++KYEGGL+ F+RGY K GF+R + GI YREWAP A
Sbjct: 129 DPMLRTYNNHLDYRYGQYKRLREEIDKYEGGLEAFSRGYEKLGFSRSDAGITYREWAPGA 188

Query: 158 QEAQIIGDFNGWDGSNHQMEKNEFGTWSIKIP-DLEGKPAIAHNSRVKFRFKHGNGVWID 217
           + A +IGDFN W+ +   M +NEFG W I +P + +G PAI H SRVK R    +G+  D
Sbjct: 189 KAASLIGDFNNWNSNADIMTRNEFGVWEIFLPNNTDGSPAIPHGSRVKIRMDTPSGI-KD 248

Query: 218 RIPAWIKYATVDPKRFAAPYNGVYWDPPALERYEFKHPRPTKPEAPRVYEAHVGMSSSEP 277
            IPAWIK++   P     P+NG+Y+DPP  E+Y FKHP+P +P++ R+YEAHVGMSS+EP
Sbjct: 249 SIPAWIKFSVQAPGEI--PFNGIYYDPPEEEKYVFKHPQPKRPKSLRIYEAHVGMSSTEP 308

Query: 278 RVSSYREFADFVLPRIKENNYNTVQLMAIMEHSYYASFGYHVTNFFAVSSRSGTPEDLKY 337
            V++Y  F D VLPRIK+  YN VQ+MAI EHSYYASFGYHVTNFFA SSR GTPE+LK 
Sbjct: 309 MVNTYANFRDDVLPRIKKLGYNAVQIMAIQEHSYYASFGYHVTNFFAPSSRCGTPEELKS 368

Query: 338 LIDKAHGLGIRVLMDVVHSHASNNVTDGLNGFDVGQGTQDSYFHTGDRGYHKLWDSRIFN 397
           LID+AH LG+ VLMD+VHSHAS N  DGLN FD   GT   YFH+G RGYH +WDSR+FN
Sbjct: 369 LIDRAHELGLVVLMDIVHSHASKNTLDGLNMFD---GTDAHYFHSGPRGYHWMWDSRLFN 428

Query: 398 YANWEVLRFLLSNIRWWLEEYQFDGFRFDGVTSMLYHHHGINMAFTGNYNEYFSEATDVD 457
           Y +WEVLR+LLSN RWWLEEY+FDGFRFDGVTSM+Y HHG+++ FTGNY EYF   TDVD
Sbjct: 429 YGSWEVLRYLLSNARWWLEEYKFDGFRFDGVTSMMYTHHGLSVGFTGNYTEYFGLETDVD 488

Query: 458 AVVYLMLANNLIHSIFPDATVIAEDVSGMPGLGRPVSEGGIGFDYRLQMAIPDKWIDYLK 517
           AV YLML N++IH ++P+A  + EDVSGMP    PV +GG+GFDYRL MAI DKWI+ LK
Sbjct: 489 AVNYLMLVNDMIHGLYPEAITVGEDVSGMPTFCIPVQDGGVGFDYRLHMAIADKWIEMLK 548

Query: 518 NKTDEEWSMGEISWNLTNRRYSEKCISYAESHDQAIVGDKTISFLLMDKEMYSGMSCLTD 577
            K DE+W MG+I + LTNRR+SEKCISYAESHDQA+VGDKTI+F LMDK+MY  M+    
Sbjct: 549 -KRDEDWQMGDIIYTLTNRRWSEKCISYAESHDQALVGDKTIAFWLMDKDMYDFMAVDRP 608

Query: 578 ASPIVERGIALHKMIHFITMALGGDGYLNFMGNEFGHPEWIDFPR-----------EGNG 637
           ++P+++RGIALHKMI  ITM LGG+GYLNFMGNEFGHPEWIDFPR            GN 
Sbjct: 609 STPLIDRGIALHKMIRLITMGLGGEGYLNFMGNEFGHPEWIDFPRGEQRLSDGSVIPGNN 668

Query: 638 WSYDKCRRQWNLPDTDHLRYKFLNAFDRAMNLLDDKFSFLASSKQIVSWTGEEDKVIIFE 697
           +SYDKCRR+++L D D+LRY+ L  FD+AM  L++ + F+ S  Q +S   E D+VI+FE
Sbjct: 669 FSYDKCRRRFDLGDADYLRYRGLQEFDQAMQHLEENYGFMTSEHQFISRKDEADRVIVFE 728

Query: 698 RGDLVFVFNFHPVNTYDGYKVGCDLPGKYRVALDSDARDFGGLGRVGHDVDHFTSPEGIP 757
           RGDLVFVFNFH  ++Y  Y++GC  PGKY++ LDSD   FGG  R+    ++FT  +G+ 
Sbjct: 729 RGDLVFVFNFHWTSSYFDYRIGCSKPGKYKIVLDSDDPLFGGFNRLDRKAEYFTY-DGL- 788

Query: 758 GVPETNFNNRPNSFKVLSPARTCVVY 772
                 ++ RP SF V +P RT VVY
Sbjct: 789 ------YDERPCSFMVYAPCRTAVVY 799

BLAST of Lag0004066 vs. TAIR 10
Match: AT3G20440.2 (Alpha amylase family protein )

HSP 1 Score: 467.6 bits (1202), Expect = 2.5e-131
Identity = 236/582 (40.55%), Postives = 358/582 (61.51%), Query Frame = 0

Query: 195 PAIAHNSRVKFRFKHGNGVWIDRIPAWIKYATVDPKRFAAPYNGVYWDPPALERYEFKHP 254
           PA+ H S+ +  F   +G  ++R+PAW  Y  V P+        ++W+P     Y++K+ 
Sbjct: 334 PAVPHGSKYRLYFNTPDGP-LERVPAWATY--VQPEDEGKQAYAIHWEPSPEAAYKWKYS 393

Query: 255 RPTKPEAPRVYEAHVGMSSSEPRVSSYREFADFVLPRIKENNYNTVQLMAIMEHSYYASF 314
           +P  PE+ R+YE HVG+S SEP+VS++ EF   VLP +K   YN +QL+ + EH  Y + 
Sbjct: 394 KPKVPESLRIYECHVGISGSEPKVSTFEEFTKKVLPHVKRAGYNAIQLIGVPEHKDYFTV 453

Query: 315 GYHVTNFFAVSSRSGTPEDLKYLIDKAHGLGIRVLMDVVHSHASNNVTDGLNGFDVGQGT 374
           GY VTNFFA SSR GTP+D K L+D+AHGLG+ V +D+VHS+A+ +   GL+ FD   G+
Sbjct: 454 GYRVTNFFAASSRYGTPDDFKRLVDEAHGLGLLVFLDIVHSYAAADQMVGLSLFD---GS 513

Query: 375 QDSYFHTGDRGYHKLWDSRIFNYANWEVLRFLLSNIRWWLEEYQFDGFRFDGVTSMLYHH 434
            D YFH G RG+HK W +R+F Y + +VL FL+SN+ WW+ EYQ DG++F  + SM+Y H
Sbjct: 514 NDCYFHYGKRGHHKHWGTRMFKYGDLDVLHFLISNLNWWITEYQVDGYQFHSLASMIYTH 573

Query: 435 HGINMAFTGNYNEYFSEATDVDAVVYLMLANNLIHSIFPDATVIAEDVSGMPGLGRPVSE 494
           +G   +F  + ++Y ++  D DA++YL+LAN ++H   P+   IAED +  PGL  PVS+
Sbjct: 574 NGF-ASFNNDLDDYCNQYVDRDALMYLILANEILHVQHPNIITIAEDATYYPGLCEPVSQ 633

Query: 495 GGIGFDYRLQMAIPDKWIDYLKNKTDEEWSMGEI-SWNLTNRRYSEKCISYAESHDQAIV 554
           GG+GFDY + ++  + W+  L N  D EWSM +I S  + N+ Y++K +SYAE+H+Q+I 
Sbjct: 634 GGLGFDYYVNLSASEMWVSLLDNVPDNEWSMSKIVSTLVANKEYADKMLSYAENHNQSIS 693

Query: 555 GDKTISFLLMDKEMYSGMSCLTDASP----IVERGIALHKMIHFITMALGGDGYLNFMGN 614
           G ++ + +L     + G+    + SP    +++RGI+LHKMI  IT   GG  YLNFMGN
Sbjct: 694 GGRSFAEIL-----FGGVD---NGSPGGKELLDRGISLHKMIRLITFTSGGRAYLNFMGN 753

Query: 615 EFGHPEWIDFPREGNGWSYDKCRRQWNLPDTDHLRYKFLNAFDRAMNLLDDKFSFLASSK 674
           EFGHPE ++FP + N +S+    R+W+L ++    + F  +FD+ +  LD     L+   
Sbjct: 754 EFGHPERVEFPTQSNNFSFSLANRRWDLLESGVHHHLF--SFDKELMDLDKSKGILSRGL 813

Query: 675 QIVSWTGEEDKVIIFERGDLVFVFNFHPVNTYDGYKVGCDLPGKYRVALDSDARDFGGLG 734
             +    + + VI F RG  +F+FNFHP N+Y+ Y VG +  G+Y + L+SD   +GG G
Sbjct: 814 PSIHHVNDANMVISFSRGPFLFIFNFHPSNSYEKYDVGVEEAGEYTMILNSDEVKYGGQG 873

Query: 735 RVGHDVDHFTSPEGIPGVPETNFNNRPNSFKVLSPARTCVVY 772
            V    DH+     +        + + N  +V  P+RT  VY
Sbjct: 874 IVTE--DHY-----LQRSISKRIDGQRNCLEVFLPSRTAQVY 891

BLAST of Lag0004066 vs. TAIR 10
Match: AT3G20440.3 (Alpha amylase family protein )

HSP 1 Score: 458.4 bits (1178), Expect = 1.5e-128
Identity = 231/552 (41.85%), Postives = 345/552 (62.50%), Query Frame = 0

Query: 195 PAIAHNSRVKFRFKHGNGVWIDRIPAWIKYATVDPKRFAAPYNG-----VYWDPPALERY 254
           PA+ H S+ +  F   +G  ++R+PAW  Y  V P   A    G     ++W+P     Y
Sbjct: 334 PAVPHGSKYRLYFNTPDGP-LERVPAWATY--VQPGMTAFEDEGKQAYAIHWEPSPEAAY 393

Query: 255 EFKHPRPTKPEAPRVYEAHVGMSSSEPRVSSYREFADFVLPRIKENNYNTVQLMAIMEHS 314
           ++K+ +P  PE+ R+YE HVG+S SEP+VS++ EF   VLP +K   YN +QL+ + EH 
Sbjct: 394 KWKYSKPKVPESLRIYECHVGISGSEPKVSTFEEFTKKVLPHVKRAGYNAIQLIGVPEHK 453

Query: 315 YYASFGYHVTNFFAVSSRSGTPEDLKYLIDKAHGLGIRVLMDVVHSHASNNVTDGLNGFD 374
            Y + GY VTNFFA SSR GTP+D K L+D+AHGLG+ V +D+VHS+A+ +   GL+ FD
Sbjct: 454 DYFTVGYRVTNFFAASSRYGTPDDFKRLVDEAHGLGLLVFLDIVHSYAAADQMVGLSLFD 513

Query: 375 VGQGTQDSYFHTGDRGYHKLWDSRIFNYANWEVLRFLLSNIRWWLEEYQFDGFRFDGVTS 434
              G+ D YFH G RG+HK W +R+F Y + +VL FL+SN+ WW+ EYQ DG++F  + S
Sbjct: 514 ---GSNDCYFHYGKRGHHKHWGTRMFKYGDLDVLHFLISNLNWWITEYQVDGYQFHSLAS 573

Query: 435 MLYHHHGINMAFTGNYNEYFSEATDVDAVVYLMLANNLIHSIFPDATVIAEDVSGMPGLG 494
           M+Y H+G   +F  + ++Y ++  D DA++YL+LAN ++H   P+   IAED +  PGL 
Sbjct: 574 MIYTHNGF-ASFNNDLDDYCNQYVDRDALMYLILANEILHVQHPNIITIAEDATYYPGLC 633

Query: 495 RPVSEGGIGFDYRLQMAIPDKWIDYLKNKTDEEWSMGEI-SWNLTNRRYSEKCISYAESH 554
            PVS+GG+GFDY + ++  + W+  L N  D EWSM +I S  + N+ Y++K +SYAE+H
Sbjct: 634 EPVSQGGLGFDYYVNLSASEMWVSLLDNVPDNEWSMSKIVSTLVANKEYADKMLSYAENH 693

Query: 555 DQAIVGDKTISFLLMDKEMYSGMSCLTDASP----IVERGIALHKMIHFITMALGGDGYL 614
           +Q+I G ++ + +L     + G+    + SP    +++RGI+LHKMI  IT   GG  YL
Sbjct: 694 NQSISGGRSFAEIL-----FGGVD---NGSPGGKELLDRGISLHKMIRLITFTSGGRAYL 753

Query: 615 NFMGNEFGHPEWIDFPREGNGWSYDKCRRQWNLPDTDHLRYKFLNAFDRAMNL--LDDKF 674
           NFMGNEFGHPE ++FP + N +S+    R+W+L ++    + F  +FD+   L  LD   
Sbjct: 754 NFMGNEFGHPERVEFPTQSNNFSFSLANRRWDLLESGVHHHLF--SFDKVSELMDLDKSK 813

Query: 675 SFLASSKQIVSWTGEEDKVIIFERGDLVFVFNFHPVNTYDGYKVGCDLPGKYRVALDSDA 734
             L+     +    + + VI F RG  +F+FNFHP N+Y+ Y VG +  G+Y + L+SD 
Sbjct: 814 GILSRGLPSIHHVNDANMVISFSRGPFLFIFNFHPSNSYEKYDVGVEEAGEYTMILNSDE 868

BLAST of Lag0004066 vs. TAIR 10
Match: AT3G20440.1 (Alpha amylase family protein )

HSP 1 Score: 424.5 bits (1090), Expect = 2.4e-118
Identity = 223/582 (38.32%), Postives = 340/582 (58.42%), Query Frame = 0

Query: 195 PAIAHNSRVKFRFKHGNGVWIDRIPAWIKYATVDPKRFAAPYNGVYWDPPALERYEFKHP 254
           PA+ H S+ +  F   +G  ++R+PAW  Y  V P+        ++W+P     Y++K+ 
Sbjct: 334 PAVPHGSKYRLYFNTPDGP-LERVPAWATY--VQPEDEGKQAYAIHWEPSPEAAYKWKYS 393

Query: 255 RPTKPEAPRVYEAHVGMSSSEPRVSSYREFADFVLPRIKENNYNTVQLMAIMEHSYYASF 314
           +P  PE+ R+YE HVG+S SEP+VS++ EF                              
Sbjct: 394 KPKVPESLRIYECHVGISGSEPKVSTFEEFTK---------------------------- 453

Query: 315 GYHVTNFFAVSSRSGTPEDLKYLIDKAHGLGIRVLMDVVHSHASNNVTDGLNGFDVGQGT 374
              VTNFFA SSR GTP+D K L+D+AHGLG+ V +D+VHS+A+ +   GL+ FD   G+
Sbjct: 454 --KVTNFFAASSRYGTPDDFKRLVDEAHGLGLLVFLDIVHSYAAADQMVGLSLFD---GS 513

Query: 375 QDSYFHTGDRGYHKLWDSRIFNYANWEVLRFLLSNIRWWLEEYQFDGFRFDGVTSMLYHH 434
            D YFH G RG+HK W +R+F Y + +VL FL+SN+ WW+ EYQ DG++F  + SM+Y H
Sbjct: 514 NDCYFHYGKRGHHKHWGTRMFKYGDLDVLHFLISNLNWWITEYQVDGYQFHSLASMIYTH 573

Query: 435 HGINMAFTGNYNEYFSEATDVDAVVYLMLANNLIHSIFPDATVIAEDVSGMPGLGRPVSE 494
           +G   +F  + ++Y ++  D DA++YL+LAN ++H   P+   IAED +  PGL  PVS+
Sbjct: 574 NGF-ASFNNDLDDYCNQYVDRDALMYLILANEILHVQHPNIITIAEDATYYPGLCEPVSQ 633

Query: 495 GGIGFDYRLQMAIPDKWIDYLKNKTDEEWSMGEI-SWNLTNRRYSEKCISYAESHDQAIV 554
           GG+GFDY + ++  + W+  L N  D EWSM +I S  + N+ Y++K +SYAE+H+Q+I 
Sbjct: 634 GGLGFDYYVNLSASEMWVSLLDNVPDNEWSMSKIVSTLVANKEYADKMLSYAENHNQSIS 693

Query: 555 GDKTISFLLMDKEMYSGMSCLTDASP----IVERGIALHKMIHFITMALGGDGYLNFMGN 614
           G ++ + +L     + G+    + SP    +++RGI+LHKMI  IT   GG  YLNFMGN
Sbjct: 694 GGRSFAEIL-----FGGVD---NGSPGGKELLDRGISLHKMIRLITFTSGGRAYLNFMGN 753

Query: 615 EFGHPEWIDFPREGNGWSYDKCRRQWNLPDTDHLRYKFLNAFDRAMNLLDDKFSFLASSK 674
           EFGHPE ++FP + N +S+    R+W+L ++    + F  +FD+ +  LD     L+   
Sbjct: 754 EFGHPERVEFPTQSNNFSFSLANRRWDLLESGVHHHLF--SFDKELMDLDKSKGILSRGL 813

Query: 675 QIVSWTGEEDKVIIFERGDLVFVFNFHPVNTYDGYKVGCDLPGKYRVALDSDARDFGGLG 734
             +    + + VI F RG  +F+FNFHP N+Y+ Y VG +  G+Y + L+SD   +GG G
Sbjct: 814 PSIHHVNDANMVISFSRGPFLFIFNFHPSNSYEKYDVGVEEAGEYTMILNSDEVKYGGQG 861

Query: 735 RVGHDVDHFTSPEGIPGVPETNFNNRPNSFKVLSPARTCVVY 772
            V    DH+     +        + + N  +V  P+RT  VY
Sbjct: 874 IVTE--DHY-----LQRSISKRIDGQRNCLEVFLPSRTAQVY 861