Lag0004021 (gene) Sponge gourd (AG‐4) v1

Overview


Name	Lag0004021
Type	gene
Organism	Luffa acutangula (Sponge gourd (AG‐4) v1)
Description	alpha-dioxygenase 1-like
Location	chr6: 451483 .. 453015 (-)
RNA-Seq Expression	Lag0004021
Synteny	Lag0004021

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: polypeptideexonCDS

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGTTATCTGAGTTAATGAAACCCATTAGAGCTCTTTTACTCCAACACTTAGTACATAAAGATTTCCATGAACTTGTTCACAGAATGACCATCCTTGATACTCTCCTCTTCTTGGTAACTTTTTTTCTTCTATCATTTTAAAAAAATCTCATGATATGTTTTTTTTTTTTTTTTTTTTTTTTTATTGAAATGACGGATAAAATTTGACATGGCACAATTGATGTATAAAATTAACAATACTGCATATACGATATCGTAGGATCAAATTTATTAGACTATTGTTATTAACATTCATCTCAACTCAAAATTGTTTATGATTATGTCAACAATATTTTTGACTTGAAATGAATGGTATCAACGGTCGTATATTATCTTAGCTGACTATATTATGCTGATGCAGATTGTGCAATATTTTGATAAGTTGGGTGTTTGGTACAAATTACCAGTGGCTTTGGGGCTACTTCATTTATCAATTCGGCGACATCTTTATCAACGATATAACTTGTTCAACGTAGGCAGAATCCCCACCGGAGTTAGGTTTAAGGCCGTTGATTTTCGTTACAGAACCGCCGACGGCAAGTATAACGATCCCTTTAACGAAGCTGCTGGTGCCTATGGATCCTTCTTTGGCAGAAACATCCTCCCTCTCGACCAAACCAAAAAAGTATATCTCCCTCATTTTAAAAAAAAGTCTCCAACATCCCATTTGATCAAATTTAGTTTACAAACACTATTTTTCTATCTACGAGTTTATCATTAAACATCCTCCCTCTCAATCAAACCAAAAAAAAAACAAGTGTTTGTTAACGTGTTTTTGTAGGTACTAAGTCCGGACCCAATGGTGGTTGCTACAAAGCTTTTGGGAAGAAGAAAGTTCATAGATACAGGGAAGCAATTCAATATGATTGCTGTTTCTTGGATTCAATTCATGATTCATGATTGGATTGATCATCTAGAGGATACCAAACAGGTCCAACATTATACCAAAGTTTAATTATTTATAATTATAATTGAAATTTTTAATATTAAAATGATTGATCATGTATAATAATTTTTGCAAGCATAGGTTGAGTTGGTTGCTCCATGTGAAGTTGCCAATGAATGCCCACTGAAATCATTCAAGTTCTACAGAACTAAACAGATGGACACTGGATCATATGAAATCAAAACCGGATCTCTCAACATTCGAACCCCTTGGTGGTAATGGTAAGATAAGATTTATTTTCACTCATAAACAAAAACATTTTCATGTATTCCGTGAGCCAGTGAAATTAATTAAAAGCACGTAGGGATGCAAGTGTTGTATATGCATGGGAATGATGAAAAAAGTTTGAAGAAGGTGAGAAGTTACAAAGATGGGAAGTTGAAAATAGGGGACGACGGCGTAGCCATCTCCGGCGACGTCCGCAACAGCTATATCGCCATGCGAGGCTTGTAACTTCTGCAGTGATTGCTAAGGTTCACACCATAGATTGGAGCCTTCAGCTCACAAACACTCATACTTTGCTTGCCGGACTCCGTGCCAATTGGTAA

mRNA sequence

ATGTTATCTGAGTTAATGAAACCCATTAGAGCTCTTTTACTCCAACACTTAGTACATAAAGATTTCCATGAACTTGTTCACAGAATGACCATCCTTGATACTCTCCTCTTCTTGATTGTGCAATATTTTGATAAGTTGGGTGTTTGGTACAAATTACCAGTGGCTTTGGGGCTACTTCATTTATCAATTCGGCGACATCTTTATCAACGATATAACTTGTTCAACGTAGGCAGAATCCCCACCGGAGTTAGGTTTAAGGCCGTTGATTTTCGTTACAGAACCGCCGACGGCAAGTATAACGATCCCTTTAACGAAGCTGCTGGTGCCTATGGATCCTTCTTTGGCAGAAACATCCTCCCTCTCGACCAAACCAAAAAAGTACTAAGTCCGGACCCAATGGTGGTTGCTACAAAGCTTTTGGGAAGAAGAAAGTTCATAGATACAGGGAAGCAATTCAATATGATTGCTGTTTCTTGGATTCAATTCATGATTCATGATTGGATTGATCATCTAGAGGATACCAAACAGGTTGAGTTGGTTGCTCCATGTGAAGTTGCCAATGAATGCCCACTGAAATCATTCAAGTTCTACAGAACTAAACAGATGGACACTGGATCATATGAAATCAAAACCGGATCTCTCAACATTCGAACCCCTTGGTGGGGACGACGGCGTAGCCATCTCCGGCGACGTCCGCAACAGCTATATCGCCATGCGAGGCTTGTAACTTCTGCAGTGATTGCTAAGGTTCACACCATAGATTGGAGCCTTCAGCTCACAAACACTCATACTTTGCTTGCCGGACTCCGTGCCAATTGGTAA

Coding sequence (CDS)

ATGTTATCTGAGTTAATGAAACCCATTAGAGCTCTTTTACTCCAACACTTAGTACATAAAGATTTCCATGAACTTGTTCACAGAATGACCATCCTTGATACTCTCCTCTTCTTGATTGTGCAATATTTTGATAAGTTGGGTGTTTGGTACAAATTACCAGTGGCTTTGGGGCTACTTCATTTATCAATTCGGCGACATCTTTATCAACGATATAACTTGTTCAACGTAGGCAGAATCCCCACCGGAGTTAGGTTTAAGGCCGTTGATTTTCGTTACAGAACCGCCGACGGCAAGTATAACGATCCCTTTAACGAAGCTGCTGGTGCCTATGGATCCTTCTTTGGCAGAAACATCCTCCCTCTCGACCAAACCAAAAAAGTACTAAGTCCGGACCCAATGGTGGTTGCTACAAAGCTTTTGGGAAGAAGAAAGTTCATAGATACAGGGAAGCAATTCAATATGATTGCTGTTTCTTGGATTCAATTCATGATTCATGATTGGATTGATCATCTAGAGGATACCAAACAGGTTGAGTTGGTTGCTCCATGTGAAGTTGCCAATGAATGCCCACTGAAATCATTCAAGTTCTACAGAACTAAACAGATGGACACTGGATCATATGAAATCAAAACCGGATCTCTCAACATTCGAACCCCTTGGTGGGGACGACGGCGTAGCCATCTCCGGCGACGTCCGCAACAGCTATATCGCCATGCGAGGCTTGTAACTTCTGCAGTGATTGCTAAGGTTCACACCATAGATTGGAGCCTTCAGCTCACAAACACTCATACTTTGCTTGCCGGACTCCGTGCCAATTGGTAA

Protein sequence

MLSELMKPIRALLLQHLVHKDFHELVHRMTILDTLLFLIVQYFDKLGVWYKLPVALGLLHLSIRRHLYQRYNLFNVGRIPTGVRFKAVDFRYRTADGKYNDPFNEAAGAYGSFFGRNILPLDQTKKVLSPDPMVVATKLLGRRKFIDTGKQFNMIAVSWIQFMIHDWIDHLEDTKQVELVAPCEVANECPLKSFKFYRTKQMDTGSYEIKTGSLNIRTPWWGRRRSHLRRRPQQLYRHARLVTSAVIAKVHTIDWSLQLTNTHTLLAGLRANW

Homology

[Close]

BLAST of Lag0004021 vs. NCBI nr
Match: XP_022158084.1 (alpha-dioxygenase 1-like [Momordica charantia])

HSP 1 Score: 393.3 bits (1009), Expect = 1.7e-105
Identity = 207/344 (60.17%), Postives = 232/344 (67.44%), Query Frame = 0


Query: 1   MLSELMKPIRALL---LQHLVHKDFHELVHRMTILDTLLFLIVQYFDKLGVWYKLPVALG 60
           M  EL+K  +A L   LQ  VH+DFHE+V RMTILDT+LFLIVQ FDKLGVW+KLPV LG
Sbjct: 1   MFCELLKHSKARLRAELQQFVHRDFHEVVQRMTILDTVLFLIVQLFDKLGVWHKLPVLLG 60

Query: 61  LLHLSIRRHLYQRYNLFNVGRIPTGVRFKAVDFRYRTADGKYNDPFNEAAGAYGSFFGRN 120
           L+HL+IRRHLYQRYNL NVG  P GV F  +DF YRTADGKYNDP +EA GA GSF  RN
Sbjct: 61  LIHLAIRRHLYQRYNLLNVGTTPIGVGFDPLDFPYRTADGKYNDPVDEAIGARGSFIARN 120

Query: 121 ILPLDQTKKVLSPDPMVVATKLLGRRKFIDTGKQFNMIAVSWIQFMIHDWIDHLEDTKQV 180
           I P+DQTK +LSPDPMV+ATKLL RRKFIDTGKQFN+IA SW+QFMIHDWIDHLEDTKQ+
Sbjct: 121 IFPIDQTKNLLSPDPMVIATKLLARRKFIDTGKQFNIIAASWVQFMIHDWIDHLEDTKQI 180

Query: 181 ELVAPCEVANECPLKSFKFYRTKQMDTGSYEIKTGSLNIRTPWW------GRRRSHLRR- 240
           ELVAP EVA+ECPLKSFKFY+TKQ+ TGSYEIKTGS+NIRTPWW      G     LR+ 
Sbjct: 181 ELVAPREVASECPLKSFKFYKTKQIPTGSYEIKTGSINIRTPWWDGSVVYGNDEERLRKV 240

Query: 241 ------------------------------------------------------------ 274
                                                                       
Sbjct: 241 RSYEDGKLKIGDDGLLLHDEDGVAISGDVRNSWAGVSALQALFVKEHNAVCDALEKEYPD 300

[Close]

BLAST of Lag0004021 vs. NCBI nr
Match: KAG6581009.1 (Alpha-dioxygenase 1, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 389.8 bits (1000), Expect = 1.9e-104
Identity = 204/344 (59.30%), Postives = 237/344 (68.90%), Query Frame = 0


Query: 1   MLSELMKPIRALL---LQHLVHKDFHELVHRMTILDTLLFLIVQYFDKLGVWYKLPVALG 60
           MLS+L+KPI+A+    LQ  +HKDFHELV RMT++DT LFLIV + DKLGVW+KLPV LG
Sbjct: 1   MLSQLLKPIKAVFKSELQKFIHKDFHELVQRMTVIDTFLFLIVHFVDKLGVWHKLPVILG 60

Query: 61  LLHLSIRRHLYQRYNLFNVGRIPTGVRFKAVDFRYRTADGKYNDPFNEAAGAYGSFFGRN 120
           LL+L+IRRHL+Q YNL NVGR P GVR   VDF YRTADGKYNDPFNEA GA GSFFGRN
Sbjct: 61  LLYLAIRRHLHQEYNLINVGRTPVGVRSNPVDFPYRTADGKYNDPFNEAVGARGSFFGRN 120

Query: 121 ILPLDQTKKVLSPDPMVVATKLLGRRKFIDTGKQFNMIAVSWIQFMIHDWIDHLEDTKQV 180
           I P+DQ+ K+LSPDPMVVATKLL RRK IDTGKQFNMIA SWIQFMIHDWIDHLEDTKQV
Sbjct: 121 IQPIDQSNKLLSPDPMVVATKLLARRKLIDTGKQFNMIAASWIQFMIHDWIDHLEDTKQV 180

Query: 181 ELVAPCEVANECPLKSFKFYRTKQMDTGSYEIKTGSLNIRTPWW-------------GRR 240
           ELVAP EVA+ECPLKSFKF++TKQ+ TG ++ KTG +N+RTPWW             G+ 
Sbjct: 181 ELVAPREVASECPLKSFKFFKTKQVPTGFFQNKTGLINVRTPWWDGSVLYGSNAETLGKV 240

Query: 241 RSH--------------------------------------------------LRR---- 274
           R++                                                  L++    
Sbjct: 241 RTYKDGKLKIADDGLLLHDNDGVAISGDVRNSWAGVSTLQALFIKEHNAVCDALKKEDPD 300

[Close]

BLAST of Lag0004021 vs. NCBI nr
Match: KAG7017750.1 (Alpha-dioxygenase 1, partial [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 389.8 bits (1000), Expect = 1.9e-104
Identity = 204/344 (59.30%), Postives = 237/344 (68.90%), Query Frame = 0


Query: 1   MLSELMKPIRALL---LQHLVHKDFHELVHRMTILDTLLFLIVQYFDKLGVWYKLPVALG 60
           MLS+L+KPI+A+    LQ  +HKDFHELV RMT++DT LFLIV + DKLGVW+KLPV LG
Sbjct: 1   MLSQLLKPIKAVFKSELQKFIHKDFHELVQRMTVIDTFLFLIVHFVDKLGVWHKLPVILG 60

Query: 61  LLHLSIRRHLYQRYNLFNVGRIPTGVRFKAVDFRYRTADGKYNDPFNEAAGAYGSFFGRN 120
           LL+L+IRRHL+Q YNL NVGR P GVR   VDF YRTADGKYNDPFNEA GA GSFFGRN
Sbjct: 61  LLYLAIRRHLHQEYNLINVGRTPVGVRSNPVDFPYRTADGKYNDPFNEAVGARGSFFGRN 120

Query: 121 ILPLDQTKKVLSPDPMVVATKLLGRRKFIDTGKQFNMIAVSWIQFMIHDWIDHLEDTKQV 180
           I P+DQ+ K+LSPDPMVVATKLL RRK IDTGKQFNMIA SWIQFMIHDWIDHLEDTKQV
Sbjct: 121 IQPIDQSNKLLSPDPMVVATKLLARRKLIDTGKQFNMIAASWIQFMIHDWIDHLEDTKQV 180

Query: 181 ELVAPCEVANECPLKSFKFYRTKQMDTGSYEIKTGSLNIRTPWW-------------GRR 240
           ELVAP EVA+ECPLKSFKF++TKQ+ TG ++ KTG +N+RTPWW             G+ 
Sbjct: 181 ELVAPREVASECPLKSFKFFKTKQVPTGFFQNKTGLINVRTPWWDGSVLYGSNAETLGKV 240

Query: 241 RSH--------------------------------------------------LRR---- 274
           R++                                                  L++    
Sbjct: 241 RTYKDGKLKIADDGLLLHDNDGVAISGDVRNSWAGVSTLQALFIKEHNAVCDALKKEDPD 300

[Close]

BLAST of Lag0004021 vs. NCBI nr
Match: XP_038903383.1 (alpha-dioxygenase 1-like isoform X2 [Benincasa hispida])

HSP 1 Score: 389.4 bits (999), Expect = 2.5e-104
Identity = 200/343 (58.31%), Postives = 237/343 (69.10%), Query Frame = 0


Query: 1   MLSELMKPIRALL---LQHLVHKDFHELVHRMTILDTLLFLIVQYFDKLGVWYKLPVALG 60
           MLS L+KPI+A+L   LQ  +HKDFHE+V RMT++DT LFLI+ + DKLGVW+KLPV LG
Sbjct: 1   MLSHLLKPIKAVLRGELQKFIHKDFHEVVQRMTVIDTFLFLIIHFIDKLGVWHKLPVILG 60

Query: 61  LLHLSIRRHLYQRYNLFNVGRIPTGVRFKAVDFRYRTADGKYNDPFNEAAGAYGSFFGRN 120
           LL+L++RRHL+Q YNLFNVGR P GVRF  +DF YRTADGKYNDPFNE AGA G+FFGRN
Sbjct: 61  LLYLAVRRHLHQEYNLFNVGRTPVGVRFNPMDFPYRTADGKYNDPFNEGAGAQGTFFGRN 120

Query: 121 ILPLDQTKKVLSPDPMVVATKLLGRRKFIDTGKQFNMIAVSWIQFMIHDWIDHLEDTKQV 180
           I P+DQ+K +L PDPMVVATKLL RRK IDTGKQFNMIA SWIQFMIHDWIDHLEDTKQV
Sbjct: 121 IHPVDQSKMLLKPDPMVVATKLLTRRKLIDTGKQFNMIAASWIQFMIHDWIDHLEDTKQV 180

Query: 181 ELVAPCEVANECPLKSFKFYRTKQMDTGSYEIKTGSLNIRTPWW-------------GRR 240
           +LVAP EVA+ECPLKSFKF++TKQ+ TG +E KTGS+N+RTPWW             G+ 
Sbjct: 181 DLVAPREVASECPLKSFKFFKTKQVSTGFFENKTGSINVRTPWWDGSVLYGSNAEKLGKV 240

Query: 241 RSHLRRR----------------------------------------------------- 274
           R++   +                                                     
Sbjct: 241 RTYKDGKLKIADDGLLLHDNDGVAISGDVRNSWAGVSTLQALFIKEHNAICDALKADEDL 300

[Close]

BLAST of Lag0004021 vs. NCBI nr
Match: XP_008443484.1 (PREDICTED: alpha-dioxygenase 1 [Cucumis melo] >KAA0053695.1 alpha-dioxygenase 1 [Cucumis melo var. makuwa])

HSP 1 Score: 389.4 bits (999), Expect = 2.5e-104
Identity = 201/344 (58.43%), Postives = 238/344 (69.19%), Query Frame = 0


Query: 1   MLSELMKPIRALL---LQHLVHKDFHELVHRMTILDTLLFLIVQYFDKLGVWYKLPVALG 60
           MLS+L+KPI+ +L   LQ  VHKDFHE+V RMT++DT LFLI+ + DKLG+W+KLPV LG
Sbjct: 1   MLSQLLKPIKIVLRGELQKFVHKDFHEVVERMTVIDTFLFLIIHFVDKLGIWHKLPVILG 60

Query: 61  LLHLSIRRHLYQRYNLFNVGRIPTGVRFKAVDFRYRTADGKYNDPFNEAAGAYGSFFGRN 120
           LL+L++RRHL+Q YNLFNVGR P GVRF  VD+ YRTADGKYNDPFNE AGA GSFFGRN
Sbjct: 61  LLYLAVRRHLHQEYNLFNVGRTPVGVRFNPVDYPYRTADGKYNDPFNEGAGAQGSFFGRN 120

Query: 121 ILPLDQTKKVLSPDPMVVATKLLGRRKFIDTGKQFNMIAVSWIQFMIHDWIDHLEDTKQV 180
           I P+DQ+K +L PDPMVVATKLL RRK IDTGKQFNMIA SWIQFMIHDWIDHLEDTKQV
Sbjct: 121 IHPVDQSKTLLKPDPMVVATKLLARRKLIDTGKQFNMIAASWIQFMIHDWIDHLEDTKQV 180

Query: 181 ELVAPCEVANECPLKSFKFYRTKQMDTGSYEIKTGSLNIRTPWW-------------GRR 240
           ELVAP EVA+ECPLKSFKF++TKQ+ TG +E KTGS+N+RTPWW             G+ 
Sbjct: 181 ELVAPSEVASECPLKSFKFFKTKQVSTGFFENKTGSINVRTPWWDGSVLYGSNAEKLGKV 240

Query: 241 RSH--------------------------------------------------LRR---- 274
           R++                                                  L++    
Sbjct: 241 RTYKDGKLKIADDDLLLHDNDGVAISGDVRNSWAGVSTLQALFIKEHNAVCDALKKEDEN 300

[Close]

BLAST of Lag0004021 vs. ExPASy Swiss-Prot
Match: Q9SGH6 (Alpha-dioxygenase 1 OS=Arabidopsis thaliana OX=3702 GN=DOX1 PE=1 SV=1)

HSP 1 Score: 338.6 bits (867), Expect = 6.7e-92
Identity = 178/340 (52.35%), Postives = 214/340 (62.94%), Query Frame = 0


Query: 6   MKPIRAL---LLQHLVHKDFHELVHRMTILDTLLFLIVQYFDKLGVWYKLPVALGLLHLS 65
           MK I +L   +L   +HKDFHE+  RM++LD  L LIV   DK+  W+KLPV LGL +L 
Sbjct: 1   MKVITSLISSILLKFIHKDFHEIYARMSLLDRFLLLIVHGVDKMVPWHKLPVFLGLTYLE 60

Query: 66  IRRHLYQRYNLFNVGRIPTGVRFKAVDFRYRTADGKYNDPFNEAAGAYGSFFGRNILPLD 125
           +RRHL+Q+YNL NVG+ PTG+RF   ++ YRTADGK+NDPFNE  G+  SFFGRN  P+D
Sbjct: 61  VRRHLHQQYNLLNVGQTPTGIRFDPANYPYRTADGKFNDPFNEGVGSQNSFFGRNCPPVD 120

Query: 126 QTKKVLSPDPMVVATKLLGRRKFIDTGKQFNMIAVSWIQFMIHDWIDHLEDTKQVELVAP 185
           Q  K+  PDPMVVATKLLGR+KFIDTGKQFNMIA SWIQFMIHDWIDHLEDT Q+ELVAP
Sbjct: 121 QKSKLRRPDPMVVATKLLGRKKFIDTGKQFNMIAASWIQFMIHDWIDHLEDTHQIELVAP 180

Query: 186 CEVANECPLKSFKFYRTKQMDTGSYEIKTGSLNIRTPWW------GRRRSHLRR------ 245
            EVA++CPL SF+F +TK++ TG +EIKTGS NIRTPWW      G     L R      
Sbjct: 181 KEVASKCPLSSFRFLKTKEVPTGFFEIKTGSQNIRTPWWDSSVIYGSNSKTLDRVRTYKD 240

Query: 246 ---------------------------------------------------------RPQ 274
                                                                      +
Sbjct: 241 GKLKISEETGLLLHDEDGLAISGDIRNSWAGVSALQALFIKEHNAVCDALKDEDDDLEDE 300

[Close]

BLAST of Lag0004021 vs. ExPASy Swiss-Prot
Match: Q9C9U3 (Alpha-dioxygenase 2 OS=Arabidopsis thaliana OX=3702 GN=DOX2 PE=2 SV=1)

HSP 1 Score: 265.0 bits (676), Expect = 9.4e-70
Identity = 147/324 (45.37%), Postives = 185/324 (57.10%), Query Frame = 0


Query: 18  VHKDFHELVHRMTILDTLLFLIVQYFDKLGVWYKLPVALGLLHLSIRRHLYQRYNLFNVG 77
           +H   H +V +M+  D  LF IV   DKLG+W++ PV LG+ +L +RRHL+QRYNL +VG
Sbjct: 11  LHPQLHHVVSKMSYFDAFLFYIVHLVDKLGLWHRFPVLLGVAYLGLRRHLHQRYNLVHVG 70

Query: 78  RIPTGVRFKAVDFRYRTADGKYNDPFNEAAGAYGSFFGRNILPLDQTKKVLSPDPMVVAT 137
            I  G  +   +F YRTADGK N P +   G+ GSF GRN+ P      +L P P VVAT
Sbjct: 71  PI-NGQGYDTDEFCYRTADGKCNHPSDNTIGSQGSFIGRNMPPSTSQYGILDPHPSVVAT 130

Query: 138 KLLGRRKFIDTGKQFNMIAVSWIQFMIHDWIDHLEDTKQVELVAPCEVANECPLKSFKFY 197
           KLL R++FID G QFN+IA SWIQFMIHDW+DHLEDT Q+EL AP EVA+ CPLKSFKF 
Sbjct: 131 KLLARKRFIDNGDQFNVIACSWIQFMIHDWVDHLEDTHQIELEAPEEVASGCPLKSFKFL 190

Query: 198 RTKQMDTGSYEIKTGSLNIRTPWW-----------GRRRSHL------------------ 257
           RTK++ T  +  K+G++N RTPWW           G RR  +                  
Sbjct: 191 RTKKVPTDDHH-KSGAVNTRTPWWDGSVIYGNDETGMRRVRVFKDGKLKISGDGLLERDE 250

Query: 258 -----------------------------------RRRP----QQLYRHARLVTSAVIAK 274
                                               R P    ++LYR ARLVT+AVIAK
Sbjct: 251 RGVPISGDIRNSWSGFSLLQALFVKEHNSVCDMLKERYPDFDDEKLYRTARLVTAAVIAK 310

[Close]

BLAST of Lag0004021 vs. ExPASy TrEMBL
Match: A0A6J1DYD0 (alpha-dioxygenase 1-like OS=Momordica charantia OX=3673 GN=LOC111024652 PE=4 SV=1)

HSP 1 Score: 393.3 bits (1009), Expect = 8.4e-106
Identity = 207/344 (60.17%), Postives = 232/344 (67.44%), Query Frame = 0


Query: 1   MLSELMKPIRALL---LQHLVHKDFHELVHRMTILDTLLFLIVQYFDKLGVWYKLPVALG 60
           M  EL+K  +A L   LQ  VH+DFHE+V RMTILDT+LFLIVQ FDKLGVW+KLPV LG
Sbjct: 1   MFCELLKHSKARLRAELQQFVHRDFHEVVQRMTILDTVLFLIVQLFDKLGVWHKLPVLLG 60

Query: 61  LLHLSIRRHLYQRYNLFNVGRIPTGVRFKAVDFRYRTADGKYNDPFNEAAGAYGSFFGRN 120
           L+HL+IRRHLYQRYNL NVG  P GV F  +DF YRTADGKYNDP +EA GA GSF  RN
Sbjct: 61  LIHLAIRRHLYQRYNLLNVGTTPIGVGFDPLDFPYRTADGKYNDPVDEAIGARGSFIARN 120

Query: 121 ILPLDQTKKVLSPDPMVVATKLLGRRKFIDTGKQFNMIAVSWIQFMIHDWIDHLEDTKQV 180
           I P+DQTK +LSPDPMV+ATKLL RRKFIDTGKQFN+IA SW+QFMIHDWIDHLEDTKQ+
Sbjct: 121 IFPIDQTKNLLSPDPMVIATKLLARRKFIDTGKQFNIIAASWVQFMIHDWIDHLEDTKQI 180

Query: 181 ELVAPCEVANECPLKSFKFYRTKQMDTGSYEIKTGSLNIRTPWW------GRRRSHLRR- 240
           ELVAP EVA+ECPLKSFKFY+TKQ+ TGSYEIKTGS+NIRTPWW      G     LR+ 
Sbjct: 181 ELVAPREVASECPLKSFKFYKTKQIPTGSYEIKTGSINIRTPWWDGSVVYGNDEERLRKV 240

Query: 241 ------------------------------------------------------------ 274
                                                                       
Sbjct: 241 RSYEDGKLKIGDDGLLLHDEDGVAISGDVRNSWAGVSALQALFVKEHNAVCDALEKEYPD 300

[Close]

BLAST of Lag0004021 vs. ExPASy TrEMBL
Match: A0A5A7UJ02 (Alpha-dioxygenase 1 OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold135G00690 PE=4 SV=1)

HSP 1 Score: 389.4 bits (999), Expect = 1.2e-104
Identity = 201/344 (58.43%), Postives = 238/344 (69.19%), Query Frame = 0


Query: 1   MLSELMKPIRALL---LQHLVHKDFHELVHRMTILDTLLFLIVQYFDKLGVWYKLPVALG 60
           MLS+L+KPI+ +L   LQ  VHKDFHE+V RMT++DT LFLI+ + DKLG+W+KLPV LG
Sbjct: 1   MLSQLLKPIKIVLRGELQKFVHKDFHEVVERMTVIDTFLFLIIHFVDKLGIWHKLPVILG 60

Query: 61  LLHLSIRRHLYQRYNLFNVGRIPTGVRFKAVDFRYRTADGKYNDPFNEAAGAYGSFFGRN 120
           LL+L++RRHL+Q YNLFNVGR P GVRF  VD+ YRTADGKYNDPFNE AGA GSFFGRN
Sbjct: 61  LLYLAVRRHLHQEYNLFNVGRTPVGVRFNPVDYPYRTADGKYNDPFNEGAGAQGSFFGRN 120

Query: 121 ILPLDQTKKVLSPDPMVVATKLLGRRKFIDTGKQFNMIAVSWIQFMIHDWIDHLEDTKQV 180
           I P+DQ+K +L PDPMVVATKLL RRK IDTGKQFNMIA SWIQFMIHDWIDHLEDTKQV
Sbjct: 121 IHPVDQSKTLLKPDPMVVATKLLARRKLIDTGKQFNMIAASWIQFMIHDWIDHLEDTKQV 180

Query: 181 ELVAPCEVANECPLKSFKFYRTKQMDTGSYEIKTGSLNIRTPWW-------------GRR 240
           ELVAP EVA+ECPLKSFKF++TKQ+ TG +E KTGS+N+RTPWW             G+ 
Sbjct: 181 ELVAPSEVASECPLKSFKFFKTKQVSTGFFENKTGSINVRTPWWDGSVLYGSNAEKLGKV 240

Query: 241 RSH--------------------------------------------------LRR---- 274
           R++                                                  L++    
Sbjct: 241 RTYKDGKLKIADDDLLLHDNDGVAISGDVRNSWAGVSTLQALFIKEHNAVCDALKKEDEN 300

[Close]

BLAST of Lag0004021 vs. ExPASy TrEMBL
Match: A0A5D3CRF7 (Alpha-dioxygenase 1 OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold1119G00040 PE=4 SV=1)

HSP 1 Score: 389.4 bits (999), Expect = 1.2e-104
Identity = 201/344 (58.43%), Postives = 238/344 (69.19%), Query Frame = 0


Query: 1   MLSELMKPIRALL---LQHLVHKDFHELVHRMTILDTLLFLIVQYFDKLGVWYKLPVALG 60
           MLS+L+KPI+ +L   LQ  VHKDFHE+V RMT++DT LFLI+ + DKLG+W+KLPV LG
Sbjct: 1   MLSQLLKPIKIVLRGELQKFVHKDFHEVVERMTVIDTFLFLIIHFVDKLGIWHKLPVILG 60

Query: 61  LLHLSIRRHLYQRYNLFNVGRIPTGVRFKAVDFRYRTADGKYNDPFNEAAGAYGSFFGRN 120
           LL+L++RRHL+Q YNLFNVGR P GVRF  VD+ YRTADGKYNDPFNE AGA GSFFGRN
Sbjct: 61  LLYLAVRRHLHQEYNLFNVGRTPVGVRFNPVDYPYRTADGKYNDPFNEGAGAQGSFFGRN 120

Query: 121 ILPLDQTKKVLSPDPMVVATKLLGRRKFIDTGKQFNMIAVSWIQFMIHDWIDHLEDTKQV 180
           I P+DQ+K +L PDPMVVATKLL RRK IDTGKQFNMIA SWIQFMIHDWIDHLEDTKQV
Sbjct: 121 IHPVDQSKTLLKPDPMVVATKLLARRKLIDTGKQFNMIAASWIQFMIHDWIDHLEDTKQV 180

Query: 181 ELVAPCEVANECPLKSFKFYRTKQMDTGSYEIKTGSLNIRTPWW-------------GRR 240
           ELVAP EVA+ECPLKSFKF++TKQ+ TG +E KTGS+N+RTPWW             G+ 
Sbjct: 181 ELVAPSEVASECPLKSFKFFKTKQVSTGFFENKTGSINVRTPWWDGSVLYGSNAEKLGKV 240

Query: 241 RSH--------------------------------------------------LRR---- 274
           R++                                                  L++    
Sbjct: 241 RTYKDGKLKIADDDLLLHDNDGVAISGDVRNSWAGVSTLQALFIKEHNAVCDALKKEDEN 300

[Close]

BLAST of Lag0004021 vs. ExPASy TrEMBL
Match: A0A1S3B8X6 (alpha-dioxygenase 1 OS=Cucumis melo OX=3656 GN=LOC103487064 PE=4 SV=1)

HSP 1 Score: 389.4 bits (999), Expect = 1.2e-104
Identity = 201/344 (58.43%), Postives = 238/344 (69.19%), Query Frame = 0


Query: 1   MLSELMKPIRALL---LQHLVHKDFHELVHRMTILDTLLFLIVQYFDKLGVWYKLPVALG 60
           MLS+L+KPI+ +L   LQ  VHKDFHE+V RMT++DT LFLI+ + DKLG+W+KLPV LG
Sbjct: 1   MLSQLLKPIKIVLRGELQKFVHKDFHEVVERMTVIDTFLFLIIHFVDKLGIWHKLPVILG 60

Query: 61  LLHLSIRRHLYQRYNLFNVGRIPTGVRFKAVDFRYRTADGKYNDPFNEAAGAYGSFFGRN 120
           LL+L++RRHL+Q YNLFNVGR P GVRF  VD+ YRTADGKYNDPFNE AGA GSFFGRN
Sbjct: 61  LLYLAVRRHLHQEYNLFNVGRTPVGVRFNPVDYPYRTADGKYNDPFNEGAGAQGSFFGRN 120

Query: 121 ILPLDQTKKVLSPDPMVVATKLLGRRKFIDTGKQFNMIAVSWIQFMIHDWIDHLEDTKQV 180
           I P+DQ+K +L PDPMVVATKLL RRK IDTGKQFNMIA SWIQFMIHDWIDHLEDTKQV
Sbjct: 121 IHPVDQSKTLLKPDPMVVATKLLARRKLIDTGKQFNMIAASWIQFMIHDWIDHLEDTKQV 180

Query: 181 ELVAPCEVANECPLKSFKFYRTKQMDTGSYEIKTGSLNIRTPWW-------------GRR 240
           ELVAP EVA+ECPLKSFKF++TKQ+ TG +E KTGS+N+RTPWW             G+ 
Sbjct: 181 ELVAPSEVASECPLKSFKFFKTKQVSTGFFENKTGSINVRTPWWDGSVLYGSNAEKLGKV 240

Query: 241 RSH--------------------------------------------------LRR---- 274
           R++                                                  L++    
Sbjct: 241 RTYKDGKLKIADDDLLLHDNDGVAISGDVRNSWAGVSTLQALFIKEHNAVCDALKKEDEN 300

[Close]

BLAST of Lag0004021 vs. ExPASy TrEMBL
Match: A0A0A0LG46 (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_3G829270 PE=4 SV=1)

HSP 1 Score: 389.0 bits (998), Expect = 1.6e-104
Identity = 202/344 (58.72%), Postives = 238/344 (69.19%), Query Frame = 0


Query: 1   MLSELMKPIRALL---LQHLVHKDFHELVHRMTILDTLLFLIVQYFDKLGVWYKLPVALG 60
           MLS+L+KPI+ +L   LQ  VHKDFHE+V RMT++DT LFLIV + DKLG+W+KLPV LG
Sbjct: 1   MLSQLLKPIKIVLRGELQKFVHKDFHEVVQRMTVIDTFLFLIVHFVDKLGIWHKLPVILG 60

Query: 61  LLHLSIRRHLYQRYNLFNVGRIPTGVRFKAVDFRYRTADGKYNDPFNEAAGAYGSFFGRN 120
           LL+L++RRHL+Q YNLFNVGR P GVRF  VDF YRTADGKYNDPFNE AGA GSFFGRN
Sbjct: 61  LLYLAVRRHLHQEYNLFNVGRTPVGVRFNPVDFPYRTADGKYNDPFNEGAGAQGSFFGRN 120

Query: 121 ILPLDQTKKVLSPDPMVVATKLLGRRKFIDTGKQFNMIAVSWIQFMIHDWIDHLEDTKQV 180
           I P+DQ+K +L PDPMVVATKLL RRK IDTGKQFNMIA SWIQFMIHDWIDHLEDTKQV
Sbjct: 121 IHPVDQSKTLLKPDPMVVATKLLARRKLIDTGKQFNMIAASWIQFMIHDWIDHLEDTKQV 180

Query: 181 ELVAPCEVANECPLKSFKFYRTKQMDTGSYEIKTGSLNIRTPWW-------------GRR 240
           ELVAP EVA+ECPLKSFKF++TKQ+ TG ++ KTGS+N+RTPWW             G+ 
Sbjct: 181 ELVAPREVASECPLKSFKFFKTKQVSTGFFDNKTGSINVRTPWWDGSVLYGSNAEKLGKV 240

Query: 241 RSH--------------------------------------------------LRR---- 274
           R++                                                  L++    
Sbjct: 241 RTYKDGKLKIADDDLLLHDNDGVAISGDVRNSWAGVSTLQALFIREHNAVCDALKKEDED 300

[Close]

BLAST of Lag0004021 vs. TAIR 10
Match: AT3G01420.1 (Peroxidase superfamily protein )

HSP 1 Score: 338.6 bits (867), Expect = 4.7e-93
Identity = 178/340 (52.35%), Postives = 214/340 (62.94%), Query Frame = 0


Query: 6   MKPIRAL---LLQHLVHKDFHELVHRMTILDTLLFLIVQYFDKLGVWYKLPVALGLLHLS 65
           MK I +L   +L   +HKDFHE+  RM++LD  L LIV   DK+  W+KLPV LGL +L 
Sbjct: 1   MKVITSLISSILLKFIHKDFHEIYARMSLLDRFLLLIVHGVDKMVPWHKLPVFLGLTYLE 60

Query: 66  IRRHLYQRYNLFNVGRIPTGVRFKAVDFRYRTADGKYNDPFNEAAGAYGSFFGRNILPLD 125
           +RRHL+Q+YNL NVG+ PTG+RF   ++ YRTADGK+NDPFNE  G+  SFFGRN  P+D
Sbjct: 61  VRRHLHQQYNLLNVGQTPTGIRFDPANYPYRTADGKFNDPFNEGVGSQNSFFGRNCPPVD 120

Query: 126 QTKKVLSPDPMVVATKLLGRRKFIDTGKQFNMIAVSWIQFMIHDWIDHLEDTKQVELVAP 185
           Q  K+  PDPMVVATKLLGR+KFIDTGKQFNMIA SWIQFMIHDWIDHLEDT Q+ELVAP
Sbjct: 121 QKSKLRRPDPMVVATKLLGRKKFIDTGKQFNMIAASWIQFMIHDWIDHLEDTHQIELVAP 180

Query: 186 CEVANECPLKSFKFYRTKQMDTGSYEIKTGSLNIRTPWW------GRRRSHLRR------ 245
            EVA++CPL SF+F +TK++ TG +EIKTGS NIRTPWW      G     L R      
Sbjct: 181 KEVASKCPLSSFRFLKTKEVPTGFFEIKTGSQNIRTPWWDSSVIYGSNSKTLDRVRTYKD 240

Query: 246 ---------------------------------------------------------RPQ 274
                                                                      +
Sbjct: 241 GKLKISEETGLLLHDEDGLAISGDIRNSWAGVSALQALFIKEHNAVCDALKDEDDDLEDE 300

[Close]

BLAST of Lag0004021 vs. TAIR 10
Match: AT1G73680.1 (alpha dioxygenase )

HSP 1 Score: 265.0 bits (676), Expect = 6.7e-71
Identity = 147/324 (45.37%), Postives = 185/324 (57.10%), Query Frame = 0


Query: 18  VHKDFHELVHRMTILDTLLFLIVQYFDKLGVWYKLPVALGLLHLSIRRHLYQRYNLFNVG 77
           +H   H +V +M+  D  LF IV   DKLG+W++ PV LG+ +L +RRHL+QRYNL +VG
Sbjct: 11  LHPQLHHVVSKMSYFDAFLFYIVHLVDKLGLWHRFPVLLGVAYLGLRRHLHQRYNLVHVG 70

Query: 78  RIPTGVRFKAVDFRYRTADGKYNDPFNEAAGAYGSFFGRNILPLDQTKKVLSPDPMVVAT 137
            I  G  +   +F YRTADGK N P +   G+ GSF GRN+ P      +L P P VVAT
Sbjct: 71  PI-NGQGYDTDEFCYRTADGKCNHPSDNTIGSQGSFIGRNMPPSTSQYGILDPHPSVVAT 130

Query: 138 KLLGRRKFIDTGKQFNMIAVSWIQFMIHDWIDHLEDTKQVELVAPCEVANECPLKSFKFY 197
           KLL R++FID G QFN+IA SWIQFMIHDW+DHLEDT Q+EL AP EVA+ CPLKSFKF 
Sbjct: 131 KLLARKRFIDNGDQFNVIACSWIQFMIHDWVDHLEDTHQIELEAPEEVASGCPLKSFKFL 190

Query: 198 RTKQMDTGSYEIKTGSLNIRTPWW-----------GRRRSHL------------------ 257
           RTK++ T  +  K+G++N RTPWW           G RR  +                  
Sbjct: 191 RTKKVPTDDHH-KSGAVNTRTPWWDGSVIYGNDETGMRRVRVFKDGKLKISGDGLLERDE 250

Query: 258 -----------------------------------RRRP----QQLYRHARLVTSAVIAK 274
                                               R P    ++LYR ARLVT+AVIAK
Sbjct: 251 RGVPISGDIRNSWSGFSLLQALFVKEHNSVCDMLKERYPDFDDEKLYRTARLVTAAVIAK 310

[Close]

BLAST of Lag0004021 vs. TAIR 10
Match: AT1G73680.2 (alpha dioxygenase )

HSP 1 Score: 249.2 bits (635), Expect = 3.8e-66
Identity = 145/333 (43.54%), Postives = 185/333 (55.56%), Query Frame = 0


Query: 18  VHKDFHELVHRMTILDTLLFLIVQYFDKLGVWYKLPVALGLLHLSIRRHLYQRYNLFNVG 77
           +H   H +V +M+  D  LF IV   DKLG+W++ PV LG+ +L +RRHL+QRYNL +VG
Sbjct: 11  LHPQLHHVVSKMSYFDAFLFYIVHLVDKLGLWHRFPVLLGVAYLGLRRHLHQRYNLVHVG 70

Query: 78  RIPTGVRFKAVDFRYRTADGKYNDPFNEAAGAYGSFFGRNILPLDQ-----TKKVLSPD- 137
            I  G  +   +F YRTADGK N P +   G+ GSF GRN+ P         +    P+ 
Sbjct: 71  PI-NGQGYDTDEFCYRTADGKCNHPSDNTIGSQGSFIGRNMPPSTSQYGFFCQYFFFPEN 130

Query: 138 ---PMVVATKLLGRRKFIDTGKQFNMIAVSWIQFMIHDWIDHLEDTKQVELVAPCEVANE 197
               +VVATKLL R++FID G QFN+IA SWIQFMIHDW+DHLEDT Q+EL AP EVA+ 
Sbjct: 131 LEKKIVVATKLLARKRFIDNGDQFNVIACSWIQFMIHDWVDHLEDTHQIELEAPEEVASG 190

Query: 198 CPLKSFKFYRTKQMDTGSYEIKTGSLNIRTPWW-----------GRRRSHL--------- 257
           CPLKSFKF RTK++ T  +  K+G++N RTPWW           G RR  +         
Sbjct: 191 CPLKSFKFLRTKKVPTDDHH-KSGAVNTRTPWWDGSVIYGNDETGMRRVRVFKDGKLKIS 250

Query: 258 --------------------------------------------RRRP----QQLYRHAR 274
                                                        R P    ++LYR AR
Sbjct: 251 GDGLLERDERGVPISGDIRNSWSGFSLLQALFVKEHNSVCDMLKERYPDFDDEKLYRTAR 310

The following BLAST results are available for this feature:

BLAST of Lag0004021 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_022158084.1	1.7e-105	60.17	alpha-dioxygenase 1-like [Momordica charantia]	[more]
KAG6581009.1	1.9e-104	59.30	Alpha-dioxygenase 1, partial [Cucurbita argyrosperma subsp. sororia]	[more]
KAG7017750.1	1.9e-104	59.30	Alpha-dioxygenase 1, partial [Cucurbita argyrosperma subsp. argyrosperma]	[more]
XP_038903383.1	2.5e-104	58.31	alpha-dioxygenase 1-like isoform X2 [Benincasa hispida]	[more]
XP_008443484.1	2.5e-104	58.43	PREDICTED: alpha-dioxygenase 1 [Cucumis melo] >KAA0053695.1 alpha-dioxygenase 1 ...	[more]

BLAST of Lag0004021 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
Q9SGH6	6.7e-92	52.35	Alpha-dioxygenase 1 OS=Arabidopsis thaliana OX=3702 GN=DOX1 PE=1 SV=1	[more]
Q9C9U3	9.4e-70	45.37	Alpha-dioxygenase 2 OS=Arabidopsis thaliana OX=3702 GN=DOX2 PE=2 SV=1	[more]

BLAST of Lag0004021 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A6J1DYD0	8.4e-106	60.17	alpha-dioxygenase 1-like OS=Momordica charantia OX=3673 GN=LOC111024652 PE=4 SV=...	[more]
A0A5A7UJ02	1.2e-104	58.43	Alpha-dioxygenase 1 OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold135G...	[more]
A0A5D3CRF7	1.2e-104	58.43	Alpha-dioxygenase 1 OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold1119...	[more]
A0A1S3B8X6	1.2e-104	58.43	alpha-dioxygenase 1 OS=Cucumis melo OX=3656 GN=LOC103487064 PE=4 SV=1	[more]
A0A0A0LG46	1.6e-104	58.72	Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_3G829270 PE=4 SV=1	[more]

BLAST of Lag0004021 vs. TAIR 10


Match Name	E-value	Identity	Description
AT3G01420.1	4.7e-93	52.35	Peroxidase superfamily protein	[more]
AT1G73680.1	6.7e-71	45.37	alpha dioxygenase	[more]
AT1G73680.2	3.8e-66	43.54	alpha dioxygenase	[more]

InterPro

Analysis Name: InterPro Annotations of Sponge gourd (AG-4) v1
Date Performed: 2022-08-01


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR019791	Haem peroxidase, animal-type	PFAM	PF03098	An_peroxidase	coord: 92..221 e-value: 8.6E-17 score: 60.9
IPR019791	Haem peroxidase, animal-type	PROSITE	PS50292	PEROXIDASE_3	coord: 82..273 score: 15.068833
IPR037120	Haem peroxidase domain superfamily, animal type	GENE3D	1.10.640.10	Haem peroxidase domain superfamily, animal type	coord: 9..222 e-value: 2.0E-46 score: 160.5
None	No IPR available	PANTHER	PTHR11903:SF27	ALPHA-DIOXYGENASE 1-LIKE	coord: 233..273
None	No IPR available	PANTHER	PTHR11903:SF27	ALPHA-DIOXYGENASE 1-LIKE	coord: 14..222
None	No IPR available	PANTHER	PTHR11903	PROSTAGLANDIN G/H SYNTHASE	coord: 14..222
None	No IPR available	PANTHER	PTHR11903	PROSTAGLANDIN G/H SYNTHASE	coord: 233..273
IPR010255	Haem peroxidase superfamily	SUPERFAMILY	48113	Heme-dependent peroxidases	coord: 89..266

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
Lag0004021.1	Lag0004021.1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0006979	response to oxidative stress
molecular_function	GO:0020037	heme binding
molecular_function	GO:0016702	oxidoreductase activity, acting on single donors with incorporation of molecular oxygen, incorporation of two atoms of oxygen
molecular_function	GO:0004601	peroxidase activity