Lag0003992 (gene) Sponge gourd (AG‐4) v1

Overview
NameLag0003992
Typegene
OrganismLuffa acutangula (Sponge gourd (AG‐4) v1)
DescriptionPurple acid phosphatase
Locationchr6: 238912 .. 241710 (-)
RNA-Seq ExpressionLag0003992
SyntenyLag0003992
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: polypeptideexonCDS
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGAAGGTATTGAATATTAATGGGTGCTCCTATTTATCTATAAAAATAAAAACTATCCTTTTTTCAAAAAAAAAAAAAAAAAAAAAAAGGAAAAACTATCCTACCCCTTATTTGATCCTATATTTGGAAATTACCCTTCACAGTTGAACCGAAGATGAAAATGAAAGATGGTGGATCAAAGTGAAGAAAGAAACAAGCGTGCAGCTGAAGAATATGCCCAGTTTCTCTGTTTTCAATCTGGTCGTCGTCGATAAGAATTCTGGTATTGCCATTGGCTTCACAGATCTCCTCTCCTGCCATCTCCTCCATACCTATTACCTGTTCTCAATCCATTCCCATGAATAATCTCATCCCTCTCATCCTTCTCATCTCCTTCCTTCCCTTTTCAACCCAATCCAAAGTCTCCATTTCTTTCTCCCCTTCAACTCTCTCCAAATCCGGCGATCCAGTCCAAATCCAATGGTCTGGAATCGAATCCCCCTCCAACCTCGACTGGCTCGGAATCTACTCGCCTCCAAATTCGTCCCACAAGCATTTCATCGGCTACGTCTTCCTTTCTTCTTCTCCCGGCTGGGAATCCGGCTTCGGATCCGTTTCCATCCCCTTAGTCAACCTCCGATCCAACTATTCATTCAGGATTTTCCGGTGGACTGAATCGGAGATCGACGAGAAGCATCAGGACCACGACCACAACCCTCTGCCTGGAACAGCGCACCTCCTCGCGGCCTCCGAGGAGCTACAATTCAGCCCCGGAGGCGGCCCGGAGCAGATCCATCTGGCGTTCACGGACCACGATGACGAAATGAGAGTGATGTTCGTGACGGAGGACGGATCGGAAAGGTATGTGAGATATGGGGAAAGGGAGAAGCTTGATCAGATTGTTGCGGCTCGTGTTCAGAGGTACGAGAGGGAGCACATGTGCGATTCCCCTGCTAATGATAGCATTGGCTGGAGGGATCCTGGTTTTATTCATGATGCGGTTATGAAGAAACTGCAAAAAGGAGCTAGGATTTATTATCAGGTAACTTGTTTGCCTAATTTGGGATTCTGTTATTTCTGCTGGAATTTGTAATGCTGTTACTTTTGGGTTTTGATCATTAACCTTGGTGGTTCAGTTAACTAAATCAAATTGTTTTTATGATGTTTGTTTGGTAAAGATTTATGCAGCTTGCTTTAAATCTCCTCAGAACTCAAGGATTCAGTGTACAATCTATAGAACAGATCTTGTTCAAGGTCGTTATGCAGCTTGTTTTTAATGTTGATTCATTTGTTCTGCTAGCCATGAGAAATCATGAAGGACATTGGTACTATATCATGGTTCTATTACATGAATCGCTGTTGGTTGACTAATTAAGATTCGAAAAGTTAAAGGCTTAATCTCATTAATGGATGCATTCAAATTAGAAATGAGATTAATGATGCTTTTTTTTTCCCTTTCTTTGTTGAGTTGACTTAATAGACCATATAGGTAAAGAACTATAATGCAATGTTGTTTGCTTTTACTAGGTTGGAAGTGACTCTAAAGGTTGGAGTAGTATTCGAAGCTTTGTGTCACGTAATGAGGACTTGGATGAGACGATTGCTTTTCTTTTCGGTGATATGGGTGCTGCAACACCGTACACGACCTTTGTCCGAACACAGGATGAAAGTATATCTACTATTAGGTGGATACTTCGTGACATTGAAGCACTTGGTGACAAACCTGCATTTGTCTCTCACATTGGAGATATAAGCTACGCAAGGGGTCATGCGTGGTTATGGGATGTTTTCTTCAATCAGATTGAACCTGTGGCATCTAAGGTTGCATACCATGTATGTATTGGCAATCACGAATATGACTGGCCTCTTCAGCCGTGGAAACCCGAGTGGGCCTACAGTATATACGGGAAAGACGGGGGAGGGGAATGTGGGGTACCATATAGTTTGAAGTTCAACATGCCAGGAAACTCTTCAGAACCTACTGCAGCCCACAGCTTGCCTACTCGAAACCTTTTCTACTCTTTTAACATGGGATCGGTGCATTTTGTGTACATATCAACAGAAACCAATTTCCTTCAAGGCAGTAGGCAATATGAATTCATAAAGCTTGATTTAGAGTCTGTTGATCGGAAGAAGACACCATTTGTAGTAGTGCAGGGGCACAGACCAATGTATACAACGAGCAACGAACTTAGAGATACCCCATTGAGGGAGAAAATGCTGCAGCATTTGGAACCATTGTTTGTGAAAAACAACGTGACATTGGCATTATGGGGTCATGTCCATAGGTATGAGAGGTTTTGCCCACTTAATAACTACACTTGTGGAAGTATGAGTGTGGATGGGGAAGAATGGGAGGCATTACCCGTCCATCTCGTTATAGGGATGGCTGGACAAGATTGGCAGCCCATCTGGGAGCCGAGACCAAACCACCCAGATGATCCGATCTTCCCACAACCGAAGCGGTCAATGTATCGTGGAGGTGAGTTCGGGTACACCAGATTGGTTGCTACGAAAGAGAAGCTTACAGTTTCTTATGTTGGAAATCATGATGGAGAGGTACACGATTCTGTGGAGATTTTGGCATCGGGACAAGTTCTAAACAGTGGAATTGATGCGAAATTCATCAACAGTAGTCTTGAAAACTCCAGGACGAGGAATGAGATGCTAGAATCCTCATTCTCCTGGTATGTGATGGGAGCAAGTATTTTGGTTCTTGGAGCCTTCATTGGATACATTATTGGATTTGTTTCACATGCAAGGAAGAATTCCATTTCAAGAAATAGCTGGACCCCTGTGAAGACTGAAGAGTTGCAATGA

mRNA sequence

ATGAAGATCTCCTCTCCTGCCATCTCCTCCATACCTATTACCTGTTCTCAATCCATTCCCATGAATAATCTCATCCCTCTCATCCTTCTCATCTCCTTCCTTCCCTTTTCAACCCAATCCAAAGTCTCCATTTCTTTCTCCCCTTCAACTCTCTCCAAATCCGGCGATCCAGTCCAAATCCAATGGTCTGGAATCGAATCCCCCTCCAACCTCGACTGGCTCGGAATCTACTCGCCTCCAAATTCGTCCCACAAGCATTTCATCGGCTACGTCTTCCTTTCTTCTTCTCCCGGCTGGGAATCCGGCTTCGGATCCGTTTCCATCCCCTTAGTCAACCTCCGATCCAACTATTCATTCAGGATTTTCCGGTGGACTGAATCGGAGATCGACGAGAAGCATCAGGACCACGACCACAACCCTCTGCCTGGAACAGCGCACCTCCTCGCGGCCTCCGAGGAGCTACAATTCAGCCCCGGAGGCGGCCCGGAGCAGATCCATCTGGCGTTCACGGACCACGATGACGAAATGAGAGTGATGTTCGTGACGGAGGACGGATCGGAAAGGTATGTGAGATATGGGGAAAGGGAGAAGCTTGATCAGATTGTTGCGGCTCGTGTTCAGAGGTACGAGAGGGAGCACATGTGCGATTCCCCTGCTAATGATAGCATTGGCTGGAGGGATCCTGGTTTTATTCATGATGCGGTTATGAAGAAACTGCAAAAAGGAGCTAGGATTTATTATCAGGTTGGAAGTGACTCTAAAGGTTGGAGTAGTATTCGAAGCTTTGTGTCACGTAATGAGGACTTGGATGAGACGATTGCTTTTCTTTTCGGTGATATGGGTGCTGCAACACCGTACACGACCTTTGTCCGAACACAGGATGAAAGTATATCTACTATTAGGTGGATACTTCGTGACATTGAAGCACTTGGTGACAAACCTGCATTTGTCTCTCACATTGGAGATATAAGCTACGCAAGGGGTCATGCGTGGTTATGGGATGTTTTCTTCAATCAGATTGAACCTGTGGCATCTAAGGTTGCATACCATGTATGTATTGGCAATCACGAATATGACTGGCCTCTTCAGCCGTGGAAACCCGAGTGGGCCTACAGTATATACGGGAAAGACGGGGGAGGGGAATGTGGGGTACCATATAGTTTGAAGTTCAACATGCCAGGAAACTCTTCAGAACCTACTGCAGCCCACAGCTTGCCTACTCGAAACCTTTTCTACTCTTTTAACATGGGATCGGTGCATTTTGTGTACATATCAACAGAAACCAATTTCCTTCAAGGCAGTAGGCAATATGAATTCATAAAGCTTGATTTAGAGTCTGTTGATCGGAAGAAGACACCATTTGTAGTAGTGCAGGGGCACAGACCAATGTATACAACGAGCAACGAACTTAGAGATACCCCATTGAGGGAGAAAATGCTGCAGCATTTGGAACCATTGTTTGTGAAAAACAACGTGACATTGGCATTATGGGGTCATGTCCATAGGTATGAGAGGTTTTGCCCACTTAATAACTACACTTGTGGAAGTATGAGTGTGGATGGGGAAGAATGGGAGGCATTACCCGTCCATCTCGTTATAGGGATGGCTGGACAAGATTGGCAGCCCATCTGGGAGCCGAGACCAAACCACCCAGATGATCCGATCTTCCCACAACCGAAGCGGTCAATGTATCGTGGAGGTGAGTTCGGGTACACCAGATTGGTTGCTACGAAAGAGAAGCTTACAGTTTCTTATGTTGGAAATCATGATGGAGAGGTACACGATTCTGTGGAGATTTTGGCATCGGGACAAGTTCTAAACAGTGGAATTGATGCGAAATTCATCAACAGTAGTCTTGAAAACTCCAGGACGAGGAATGAGATGCTAGAATCCTCATTCTCCTGGTATGTGATGGGAGCAAGTATTTTGGTTCTTGGAGCCTTCATTGGATACATTATTGGATTTGTTTCACATGCAAGGAAGAATTCCATTTCAAGAAATAGCTGGACCCCTGTGAAGACTGAAGAGTTGCAATGA

Coding sequence (CDS)

ATGAAGATCTCCTCTCCTGCCATCTCCTCCATACCTATTACCTGTTCTCAATCCATTCCCATGAATAATCTCATCCCTCTCATCCTTCTCATCTCCTTCCTTCCCTTTTCAACCCAATCCAAAGTCTCCATTTCTTTCTCCCCTTCAACTCTCTCCAAATCCGGCGATCCAGTCCAAATCCAATGGTCTGGAATCGAATCCCCCTCCAACCTCGACTGGCTCGGAATCTACTCGCCTCCAAATTCGTCCCACAAGCATTTCATCGGCTACGTCTTCCTTTCTTCTTCTCCCGGCTGGGAATCCGGCTTCGGATCCGTTTCCATCCCCTTAGTCAACCTCCGATCCAACTATTCATTCAGGATTTTCCGGTGGACTGAATCGGAGATCGACGAGAAGCATCAGGACCACGACCACAACCCTCTGCCTGGAACAGCGCACCTCCTCGCGGCCTCCGAGGAGCTACAATTCAGCCCCGGAGGCGGCCCGGAGCAGATCCATCTGGCGTTCACGGACCACGATGACGAAATGAGAGTGATGTTCGTGACGGAGGACGGATCGGAAAGGTATGTGAGATATGGGGAAAGGGAGAAGCTTGATCAGATTGTTGCGGCTCGTGTTCAGAGGTACGAGAGGGAGCACATGTGCGATTCCCCTGCTAATGATAGCATTGGCTGGAGGGATCCTGGTTTTATTCATGATGCGGTTATGAAGAAACTGCAAAAAGGAGCTAGGATTTATTATCAGGTTGGAAGTGACTCTAAAGGTTGGAGTAGTATTCGAAGCTTTGTGTCACGTAATGAGGACTTGGATGAGACGATTGCTTTTCTTTTCGGTGATATGGGTGCTGCAACACCGTACACGACCTTTGTCCGAACACAGGATGAAAGTATATCTACTATTAGGTGGATACTTCGTGACATTGAAGCACTTGGTGACAAACCTGCATTTGTCTCTCACATTGGAGATATAAGCTACGCAAGGGGTCATGCGTGGTTATGGGATGTTTTCTTCAATCAGATTGAACCTGTGGCATCTAAGGTTGCATACCATGTATGTATTGGCAATCACGAATATGACTGGCCTCTTCAGCCGTGGAAACCCGAGTGGGCCTACAGTATATACGGGAAAGACGGGGGAGGGGAATGTGGGGTACCATATAGTTTGAAGTTCAACATGCCAGGAAACTCTTCAGAACCTACTGCAGCCCACAGCTTGCCTACTCGAAACCTTTTCTACTCTTTTAACATGGGATCGGTGCATTTTGTGTACATATCAACAGAAACCAATTTCCTTCAAGGCAGTAGGCAATATGAATTCATAAAGCTTGATTTAGAGTCTGTTGATCGGAAGAAGACACCATTTGTAGTAGTGCAGGGGCACAGACCAATGTATACAACGAGCAACGAACTTAGAGATACCCCATTGAGGGAGAAAATGCTGCAGCATTTGGAACCATTGTTTGTGAAAAACAACGTGACATTGGCATTATGGGGTCATGTCCATAGGTATGAGAGGTTTTGCCCACTTAATAACTACACTTGTGGAAGTATGAGTGTGGATGGGGAAGAATGGGAGGCATTACCCGTCCATCTCGTTATAGGGATGGCTGGACAAGATTGGCAGCCCATCTGGGAGCCGAGACCAAACCACCCAGATGATCCGATCTTCCCACAACCGAAGCGGTCAATGTATCGTGGAGGTGAGTTCGGGTACACCAGATTGGTTGCTACGAAAGAGAAGCTTACAGTTTCTTATGTTGGAAATCATGATGGAGAGGTACACGATTCTGTGGAGATTTTGGCATCGGGACAAGTTCTAAACAGTGGAATTGATGCGAAATTCATCAACAGTAGTCTTGAAAACTCCAGGACGAGGAATGAGATGCTAGAATCCTCATTCTCCTGGTATGTGATGGGAGCAAGTATTTTGGTTCTTGGAGCCTTCATTGGATACATTATTGGATTTGTTTCACATGCAAGGAAGAATTCCATTTCAAGAAATAGCTGGACCCCTGTGAAGACTGAAGAGTTGCAATGA

Protein sequence

MKISSPAISSIPITCSQSIPMNNLIPLILLISFLPFSTQSKVSISFSPSTLSKSGDPVQIQWSGIESPSNLDWLGIYSPPNSSHKHFIGYVFLSSSPGWESGFGSVSIPLVNLRSNYSFRIFRWTESEIDEKHQDHDHNPLPGTAHLLAASEELQFSPGGGPEQIHLAFTDHDDEMRVMFVTEDGSERYVRYGEREKLDQIVAARVQRYEREHMCDSPANDSIGWRDPGFIHDAVMKKLQKGARIYYQVGSDSKGWSSIRSFVSRNEDLDETIAFLFGDMGAATPYTTFVRTQDESISTIRWILRDIEALGDKPAFVSHIGDISYARGHAWLWDVFFNQIEPVASKVAYHVCIGNHEYDWPLQPWKPEWAYSIYGKDGGGECGVPYSLKFNMPGNSSEPTAAHSLPTRNLFYSFNMGSVHFVYISTETNFLQGSRQYEFIKLDLESVDRKKTPFVVVQGHRPMYTTSNELRDTPLREKMLQHLEPLFVKNNVTLALWGHVHRYERFCPLNNYTCGSMSVDGEEWEALPVHLVIGMAGQDWQPIWEPRPNHPDDPIFPQPKRSMYRGGEFGYTRLVATKEKLTVSYVGNHDGEVHDSVEILASGQVLNSGIDAKFINSSLENSRTRNEMLESSFSWYVMGASILVLGAFIGYIIGFVSHARKNSISRNSWTPVKTEELQ
Homology
BLAST of Lag0003992 vs. NCBI nr
Match: XP_038890179.1 (probable inactive purple acid phosphatase 2 [Benincasa hispida])

HSP 1 Score: 1281.5 bits (3315), Expect = 0.0e+00
Identity = 608/659 (92.26%), Postives = 632/659 (95.90%), Query Frame = 0

Query: 21  MNNLIPLILLISFLPFSTQSKVSISFSPSTLSKSGDPVQIQWSGIESPSNLDWLGIYSPP 80
           MNNLIPLILLISF P S QSKVSIS SPS LSKSGD VQIQWSGIESPS LDWLGIYSPP
Sbjct: 1   MNNLIPLILLISFFPISIQSKVSISVSPSILSKSGDSVQIQWSGIESPSKLDWLGIYSPP 60

Query: 81  NSSHKHFIGYVFLSSSPGWESGFGSVSIPLVNLRSNYSFRIFRWTESEIDEKHQDHDHNP 140
           NSSHKHFIGYVFLSSSP W+SG GSVSIPLVNLRSNYSFRIFRWTESEID KH DHDHNP
Sbjct: 61  NSSHKHFIGYVFLSSSPSWQSGSGSVSIPLVNLRSNYSFRIFRWTESEIDHKHHDHDHNP 120

Query: 141 LPGTAHLLAASEELQFSPGGGPEQIHLAFTDHDDEMRVMFVTEDGSERYVRYGE-REKLD 200
           LPGTAHLLAAS EL+FSPGGGPEQIHLAFTD+DDEMRVMFVTEDGSERYVRYGE +EKLD
Sbjct: 121 LPGTAHLLAASGELRFSPGGGPEQIHLAFTDYDDEMRVMFVTEDGSERYVRYGENKEKLD 180

Query: 201 QIVAARVQRYEREHMCDSPANDSIGWRDPGFIHDAVMKKLQKGARIYYQVGSDSKGWSSI 260
           QIVAARV+RYEREHMCDSPANDSIGWRDPGFIHDAVMKKLQKGA++YYQVGSDSKGWS I
Sbjct: 181 QIVAARVERYEREHMCDSPANDSIGWRDPGFIHDAVMKKLQKGAKVYYQVGSDSKGWSRI 240

Query: 261 RSFVSRNEDLDETIAFLFGDMGAATPYTTFVRTQDESISTIRWILRDIEALGDKPAFVSH 320
            +FVSRNED DETIAFLFGDMGAATPYTTFVRTQ+ESIST+RWILRDIEALGDKPAF+SH
Sbjct: 241 LNFVSRNEDSDETIAFLFGDMGAATPYTTFVRTQEESISTVRWILRDIEALGDKPAFISH 300

Query: 321 IGDISYARGHAWLWDVFFNQIEPVASKVAYHVCIGNHEYDWPLQPWKPEWAYSIYGKDGG 380
           IGDISYARGHAWLWDVFF+QIEPVASKVAYHVCIGNHEYDWPLQPWKPEWA  IYGKDGG
Sbjct: 301 IGDISYARGHAWLWDVFFHQIEPVASKVAYHVCIGNHEYDWPLQPWKPEWANGIYGKDGG 360

Query: 381 GECGVPYSLKFNMPGNSSEPTAAHSLPTRNLFYSFNMGSVHFVYISTETNFLQGSRQYEF 440
           GECGVPYS+KFNMPGNSSEPT +HSLPTRNLFYSFNMGSVHFVYISTETNFLQGS QYEF
Sbjct: 361 GECGVPYSMKFNMPGNSSEPTESHSLPTRNLFYSFNMGSVHFVYISTETNFLQGSSQYEF 420

Query: 441 IKLDLESVDRKKTPFVVVQGHRPMYTTSNELRDTPLREKMLQHLEPLFVKNNVTLALWGH 500
           IK DLESVDRKKTPFVVVQGHRPMYTTSNELRDTPLREKML HLEPLFVKNNVTLALWGH
Sbjct: 421 IKRDLESVDRKKTPFVVVQGHRPMYTTSNELRDTPLREKMLHHLEPLFVKNNVTLALWGH 480

Query: 501 VHRYERFCPLNNYTCGSMSVDGEEWEALPVHLVIGMAGQDWQPIWEPRPNHPDDPIFPQP 560
           VHRYERFCPLNNYTCGSM VDGE+WEALPVHLVIGMAGQDWQPIWEPRPNHPDDP+FPQP
Sbjct: 481 VHRYERFCPLNNYTCGSMGVDGEDWEALPVHLVIGMAGQDWQPIWEPRPNHPDDPVFPQP 540

Query: 561 KRSMYRGGEFGYTRLVATKEKLTVSYVGNHDGEVHDSVEILASGQVLNSGIDAKFINSSL 620
           KRSMYRGGEFGYTRLVATKEKLT+SYVGNHDGEVHDSVEILASGQVLN G+ +KFINSS+
Sbjct: 541 KRSMYRGGEFGYTRLVATKEKLTISYVGNHDGEVHDSVEILASGQVLNGGVGSKFINSSI 600

Query: 621 ENSRTRNEMLESSFSWYVMGASILVLGAFIGYIIGFVSHARKNSISRNSWTPVKTEELQ 679
            NS +RNEMLESSFSW+VMGAS+LVLGAFIGYIIGFVSHARKNSISRN+WTPVKTEELQ
Sbjct: 601 TNSTSRNEMLESSFSWFVMGASLLVLGAFIGYIIGFVSHARKNSISRNNWTPVKTEELQ 659

BLAST of Lag0003992 vs. NCBI nr
Match: XP_008465701.1 (PREDICTED: probable inactive purple acid phosphatase 2 [Cucumis melo])

HSP 1 Score: 1256.1 bits (3249), Expect = 0.0e+00
Identity = 595/656 (90.70%), Postives = 620/656 (94.51%), Query Frame = 0

Query: 24  LIPLILLISFLPFSTQSKVSISFSPSTLSKSGDPVQIQWSGIESPSNLDWLGIYSPPNSS 83
           +IP ILL SF+P S QSKVSIS SPS LSKSGD V IQWSGIESPSNLDWLGIYSPPNSS
Sbjct: 5   IIPFILLFSFVPLSFQSKVSISLSPSILSKSGDSVHIQWSGIESPSNLDWLGIYSPPNSS 64

Query: 84  HKHFIGYVFLSSSPGWESGFGSVSIPLVNLRSNYSFRIFRWTESEIDEKHQDHDHNPLPG 143
           HKHF+GY+FLSSSP WESG+GSVSIPLVNLRSNYSFRIFRWTESEID+KH DHDHNPLPG
Sbjct: 65  HKHFVGYLFLSSSPTWESGYGSVSIPLVNLRSNYSFRIFRWTESEIDDKHHDHDHNPLPG 124

Query: 144 TAHLLAASEELQFSPGGGPEQIHLAFTDHDDEMRVMFVTEDGSERYVRYGE-REKLDQIV 203
           TAHLLAAS+EL+F+PGGGPEQIHLAFTD DDEMRVMFVTEDGSERYVRYGE +EKLDQIV
Sbjct: 125 TAHLLAASDELRFAPGGGPEQIHLAFTDQDDEMRVMFVTEDGSERYVRYGEKKEKLDQIV 184

Query: 204 AARVQRYEREHMCDSPANDSIGWRDPGFIHDAVMKKLQKGARIYYQVGSDSKGWSSIRSF 263
            A V+RYEREHMCDSPANDSIGWRDPGFIHDAVM KL+KGA++YYQVGSDSKGWSSI +F
Sbjct: 185 VAGVERYEREHMCDSPANDSIGWRDPGFIHDAVMNKLKKGAKVYYQVGSDSKGWSSILNF 244

Query: 264 VSRNEDLDETIAFLFGDMGAATPYTTFVRTQDESISTIRWILRDIEALGDKPAFVSHIGD 323
           VSRNED DETIAFLFGDMGAATPYTTFVRTQDESIST+RWILRDIEALGDKPA VSHIGD
Sbjct: 245 VSRNEDSDETIAFLFGDMGAATPYTTFVRTQDESISTVRWILRDIEALGDKPAMVSHIGD 304

Query: 324 ISYARGHAWLWDVFFNQIEPVASKVAYHVCIGNHEYDWPLQPWKPEWAYSIYGKDGGGEC 383
           ISYARGH+WLWDVFFNQIEPVASKVAYHVCIGNHEYDWPLQPWKPEWAY IYGKDGGGEC
Sbjct: 305 ISYARGHSWLWDVFFNQIEPVASKVAYHVCIGNHEYDWPLQPWKPEWAYGIYGKDGGGEC 364

Query: 384 GVPYSLKFNMPGNSSEPTAAHSLPTRNLFYSFNMGSVHFVYISTETNFLQGSRQYEFIKL 443
           GVPYSLKFNMPGN SEPT +HSLPTRNLFYSFNMGSVHFVYISTETNFLQGS QYEFIK 
Sbjct: 365 GVPYSLKFNMPGNFSEPTESHSLPTRNLFYSFNMGSVHFVYISTETNFLQGSSQYEFIKR 424

Query: 444 DLESVDRKKTPFVVVQGHRPMYTTSNELRDTPLREKMLQHLEPLFVKNNVTLALWGHVHR 503
           DLESVDRKKTPFVVVQGHRPMYTTSNELRD PLREKML HLEPL VKNNVTLALWGHVHR
Sbjct: 425 DLESVDRKKTPFVVVQGHRPMYTTSNELRDAPLREKMLHHLEPLLVKNNVTLALWGHVHR 484

Query: 504 YERFCPLNNYTCGSMSVDGEEWEALPVHLVIGMAGQDWQPIWEPRPNHPDDPIFPQPKRS 563
           YERFCPLNNYTCGSM +DGE+WEALPVHLVIGMAGQDWQPIWEPRPNHPDDPIFPQPKRS
Sbjct: 485 YERFCPLNNYTCGSMGLDGEDWEALPVHLVIGMAGQDWQPIWEPRPNHPDDPIFPQPKRS 544

Query: 564 MYRGGEFGYTRLVATKEKLTVSYVGNHDGEVHDSVEILASGQVLNSGIDAKFINSSLENS 623
           MYRGGEFGYTRLVATKEKLT+SYVGNHDGEVHDSVEILASGQVLN  + AKFI+SS+ NS
Sbjct: 545 MYRGGEFGYTRLVATKEKLTISYVGNHDGEVHDSVEILASGQVLNGNVGAKFIDSSIANS 604

Query: 624 RTRNEMLESSFSWYVMGASILVLGAFIGYIIGFVSHARKNSISRNSWTPVKTEELQ 679
            T N MLE SFSWYVMG SILVLGAFIGYIIGFVSHARKNSISRN+WTPVKTEELQ
Sbjct: 605 TTGNAMLEFSFSWYVMGGSILVLGAFIGYIIGFVSHARKNSISRNNWTPVKTEELQ 660

BLAST of Lag0003992 vs. NCBI nr
Match: XP_004143791.1 (probable inactive purple acid phosphatase 2 [Cucumis sativus] >KGN51195.1 hypothetical protein Csa_007744 [Cucumis sativus])

HSP 1 Score: 1252.3 bits (3239), Expect = 0.0e+00
Identity = 589/656 (89.79%), Postives = 621/656 (94.66%), Query Frame = 0

Query: 24  LIPLILLISFLPFSTQSKVSISFSPSTLSKSGDPVQIQWSGIESPSNLDWLGIYSPPNSS 83
           +IP +LL SF+P S QSKVS+SFSPS LSKSGD V IQWSGIESPS LDWLGIYSPPNSS
Sbjct: 5   IIPFVLLFSFIPISFQSKVSVSFSPSILSKSGDSVHIQWSGIESPSKLDWLGIYSPPNSS 64

Query: 84  HKHFIGYVFLSSSPGWESGFGSVSIPLVNLRSNYSFRIFRWTESEIDEKHQDHDHNPLPG 143
           HKHFIGY+FLSSSP WESG+GSVSIPLVNLRSNY+FRIFRWTESEID+KH DHDHNPLPG
Sbjct: 65  HKHFIGYLFLSSSPTWESGYGSVSIPLVNLRSNYAFRIFRWTESEIDDKHHDHDHNPLPG 124

Query: 144 TAHLLAASEELQFSPGGGPEQIHLAFTDHDDEMRVMFVTEDGSERYVRYGE-REKLDQIV 203
           TAHLLAAS+EL+F+PGGGPEQIHLAFTD DDEMRVMFVT+DGS+RYVRYGE +EKLDQIV
Sbjct: 125 TAHLLAASDELRFAPGGGPEQIHLAFTDQDDEMRVMFVTKDGSKRYVRYGEKKEKLDQIV 184

Query: 204 AARVQRYEREHMCDSPANDSIGWRDPGFIHDAVMKKLQKGARIYYQVGSDSKGWSSIRSF 263
            A V+RYEREHMCDSPANDSIGWRDPGFIHDAVM KL+KGA++YYQVGSDSKGWSSI +F
Sbjct: 185 VAGVERYEREHMCDSPANDSIGWRDPGFIHDAVMNKLKKGAKVYYQVGSDSKGWSSILNF 244

Query: 264 VSRNEDLDETIAFLFGDMGAATPYTTFVRTQDESISTIRWILRDIEALGDKPAFVSHIGD 323
           VSRNED DETIAFLFGDMGAATPYTTFVRTQDESIST+RWILRDIEALGDKPA VSHIGD
Sbjct: 245 VSRNEDSDETIAFLFGDMGAATPYTTFVRTQDESISTVRWILRDIEALGDKPAMVSHIGD 304

Query: 324 ISYARGHAWLWDVFFNQIEPVASKVAYHVCIGNHEYDWPLQPWKPEWAYSIYGKDGGGEC 383
           ISYARGH+WLWDVFFNQ+EPVASKVAYHVCIGNHEYDWPLQPWKPEWA  IYGKDGGGEC
Sbjct: 305 ISYARGHSWLWDVFFNQVEPVASKVAYHVCIGNHEYDWPLQPWKPEWANGIYGKDGGGEC 364

Query: 384 GVPYSLKFNMPGNSSEPTAAHSLPTRNLFYSFNMGSVHFVYISTETNFLQGSRQYEFIKL 443
           GVPYSLKFNMPGNS+EPT +HSLPTRNLFYSFNMGSVHFVYISTETNFLQGS QYEFIK 
Sbjct: 365 GVPYSLKFNMPGNSTEPTESHSLPTRNLFYSFNMGSVHFVYISTETNFLQGSSQYEFIKR 424

Query: 444 DLESVDRKKTPFVVVQGHRPMYTTSNELRDTPLREKMLQHLEPLFVKNNVTLALWGHVHR 503
           DLESVDRKKTPF+VVQGHRPMYTTSNELRD PLREKML HLEPL VKNNVTLALWGHVHR
Sbjct: 425 DLESVDRKKTPFIVVQGHRPMYTTSNELRDAPLREKMLHHLEPLLVKNNVTLALWGHVHR 484

Query: 504 YERFCPLNNYTCGSMSVDGEEWEALPVHLVIGMAGQDWQPIWEPRPNHPDDPIFPQPKRS 563
           YERFCPLNNYTCGSM +DGE+WEALPVHLVIGMAGQDWQPIWEPRPNHPDDPIFPQPKRS
Sbjct: 485 YERFCPLNNYTCGSMGLDGEDWEALPVHLVIGMAGQDWQPIWEPRPNHPDDPIFPQPKRS 544

Query: 564 MYRGGEFGYTRLVATKEKLTVSYVGNHDGEVHDSVEILASGQVLNSGIDAKFINSSLENS 623
           MYRGGEFGYTRLVATKEKLT+SYVGNHDGEVHDSVEILASGQVLN G+ AKFINSS+ NS
Sbjct: 545 MYRGGEFGYTRLVATKEKLTISYVGNHDGEVHDSVEILASGQVLNGGVGAKFINSSIANS 604

Query: 624 RTRNEMLESSFSWYVMGASILVLGAFIGYIIGFVSHARKNSISRNSWTPVKTEELQ 679
            T N MLE SFSWYVMG SILVLGAFIGYIIGFVSHARKNS+SRN+WTPVKTEELQ
Sbjct: 605 TTGNAMLEFSFSWYVMGGSILVLGAFIGYIIGFVSHARKNSLSRNNWTPVKTEELQ 660

BLAST of Lag0003992 vs. NCBI nr
Match: XP_022973269.1 (probable inactive purple acid phosphatase 2 [Cucurbita maxima])

HSP 1 Score: 1242.6 bits (3214), Expect = 0.0e+00
Identity = 596/671 (88.82%), Postives = 627/671 (93.44%), Query Frame = 0

Query: 9   SSIPITCSQSIPMNNLIPLILLISFLPFSTQSKVSISFSPSTLSKSGDPVQIQWSGIESP 68
           SS+  TCS  IPM NLI LILLIS  P S QSKVSIS SPSTL+KSGDPVQIQWSG+ESP
Sbjct: 18  SSVFDTCSSFIPMENLIYLILLISIFPISIQSKVSISISPSTLNKSGDPVQIQWSGVESP 77

Query: 69  SNLDWLGIYSPPNSSHKHFIGYVFLSSSPGWESGFGSVSIPLVNLRSNYSFRIFRWTESE 128
           SNLDWLGIYSPPNSSHKHFIGYVFLSSSPGWESG GSVSIPLVNLRS YSFRIFRWTESE
Sbjct: 78  SNLDWLGIYSPPNSSHKHFIGYVFLSSSPGWESGSGSVSIPLVNLRSKYSFRIFRWTESE 137

Query: 129 IDEKHQDHDHNPLPGTAHLLAASEELQFSPGGGPEQIHLAFTDHDDEMRVMFVTEDGSER 188
           ID+KH DHD+NPLPGT HLLAASEEL+FSPG GPEQIHLAFTD DDEMRVMFVTEDGSER
Sbjct: 138 IDDKHHDHDNNPLPGTKHLLAASEELRFSPGRGPEQIHLAFTDQDDEMRVMFVTEDGSER 197

Query: 189 YVRYGER-EKLDQIVAARVQRYEREHMCDSPANDSIGWRDPGFIHDAVMKKLQKGARIYY 248
           +VRYGE+ E+LDQIVAARV RYEREHMCDSPANDSIGWRDPGFIHDAVM KLQ G+R+YY
Sbjct: 198 FVRYGEKEEELDQIVAARVARYEREHMCDSPANDSIGWRDPGFIHDAVMTKLQNGSRVYY 257

Query: 249 QVGSDSKGWSSIRSFVSRNEDLDETIAFLFGDMGAATPYTTFVRTQDESISTIRWILRDI 308
           QVGS+SKGWSSI+SF+SRNED DETIAFLFGDMGAATPYTTFVRTQDESIST+RWILRDI
Sbjct: 258 QVGSESKGWSSIQSFMSRNEDSDETIAFLFGDMGAATPYTTFVRTQDESISTVRWILRDI 317

Query: 309 EALGDKPAFVSHIGDISYARGHAWLWDVFFNQIEPVASKVAYHVCIGNHEYDWPLQPWKP 368
           EALGDKPAFVSHIGDISYARGHAWLWDVFFNQIEPVAS+VAYHVCIGNHEYDWPLQPWKP
Sbjct: 318 EALGDKPAFVSHIGDISYARGHAWLWDVFFNQIEPVASRVAYHVCIGNHEYDWPLQPWKP 377

Query: 369 EWAYSIYGKDGGGECGVPYSLKFNMPGNSSEPTAAHSLPTRNLFYSFNMGSVHFVYISTE 428
           +WA  IYGKDGGGECGVPYSLKFNMPGNSSEPT  + +PTRNLFYSFNMGSVHFVYISTE
Sbjct: 378 DWASGIYGKDGGGECGVPYSLKFNMPGNSSEPTKTNGVPTRNLFYSFNMGSVHFVYISTE 437

Query: 429 TNFLQGSRQYEFIKLDLESVDRKKTPFVVVQGHRPMYTTSNELRDTPLREKMLQHLEPLF 488
           TNFL+GSRQ+ F++ DLESVDRKKTPFVVVQGHRPMYTTSNE RDTPLREKML HLEPLF
Sbjct: 438 TNFLKGSRQFSFLERDLESVDRKKTPFVVVQGHRPMYTTSNERRDTPLREKMLHHLEPLF 497

Query: 489 VKNNVTLALWGHVHRYERFCPLNNYTCGSMSVDGEEWEALPVHLVIGMAGQDWQPIWEPR 548
           VKNNVTLALWGHVHRYERFCPL NYTCGSM VDGE+WEALPVHLVIGMAGQDWQPIWEPR
Sbjct: 498 VKNNVTLALWGHVHRYERFCPLKNYTCGSMGVDGEDWEALPVHLVIGMAGQDWQPIWEPR 557

Query: 549 PNHPDDPIFPQPKRSMYRGGEFGYTRLVATKEKLTVSYVGNHDGEVHDSVEILASGQVLN 608
           PNH D PIFPQPKRSMYRGGEFGYTRLVATKEKLT+SYVGNHDGEVHDSVEILASGQV+N
Sbjct: 558 PNHLDVPIFPQPKRSMYRGGEFGYTRLVATKEKLTISYVGNHDGEVHDSVEILASGQVVN 617

Query: 609 SGIDAKFINSSLENSRTRNEMLESSFSWYVMGASILVLGAFIGYIIGFVSHARKNSISRN 668
            G+ AKF N S+ENSR+RN MLESSFSWYVMG SILVLGAFIGYI+GFVSHARK   SRN
Sbjct: 618 GGVGAKF-NESVENSRSRNGMLESSFSWYVMGGSILVLGAFIGYIVGFVSHARK---SRN 677

Query: 669 SWTPVKTEELQ 679
           +WTPVKTEELQ
Sbjct: 678 NWTPVKTEELQ 684

BLAST of Lag0003992 vs. NCBI nr
Match: KAG6585839.1 (putative inactive purple acid phosphatase 2, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 1239.2 bits (3205), Expect = 0.0e+00
Identity = 595/671 (88.67%), Postives = 626/671 (93.29%), Query Frame = 0

Query: 9   SSIPITCSQSIPMNNLIPLILLISFLPFSTQSKVSISFSPSTLSKSGDPVQIQWSGIESP 68
           SS+  TCS  IPM NLI LILLIS  P S QSKVSIS SPSTL+KSGDPVQIQWSG+ESP
Sbjct: 18  SSVFDTCSSFIPMENLIYLILLISIFPISIQSKVSISISPSTLNKSGDPVQIQWSGVESP 77

Query: 69  SNLDWLGIYSPPNSSHKHFIGYVFLSSSPGWESGFGSVSIPLVNLRSNYSFRIFRWTESE 128
           SNLDWLGIYSPPNSSHKHFIGYVFLSSSPGWESG GSVSIPLVNLRS YSFRIFRWTESE
Sbjct: 78  SNLDWLGIYSPPNSSHKHFIGYVFLSSSPGWESGSGSVSIPLVNLRSKYSFRIFRWTESE 137

Query: 129 IDEKHQDHDHNPLPGTAHLLAASEELQFSPGGGPEQIHLAFTDHDDEMRVMFVTEDGSER 188
           ID+KH DHD+NPLPGT HLLA+SEEL FSPG GPEQIHLAFTD DDEMRVMFVTEDGSER
Sbjct: 138 IDDKHHDHDNNPLPGTKHLLASSEELGFSPGRGPEQIHLAFTDQDDEMRVMFVTEDGSER 197

Query: 189 YVRYGER-EKLDQIVAARVQRYEREHMCDSPANDSIGWRDPGFIHDAVMKKLQKGARIYY 248
            VRYGE+ E+LDQIVAARV RYER+HMCDSPANDSIGWRDPGFIHDAVM  LQ G+R+YY
Sbjct: 198 LVRYGEKEEELDQIVAARVARYERKHMCDSPANDSIGWRDPGFIHDAVMTNLQNGSRVYY 257

Query: 249 QVGSDSKGWSSIRSFVSRNEDLDETIAFLFGDMGAATPYTTFVRTQDESISTIRWILRDI 308
           QVGS+SKGWSSI+SF+SRNED DETIAFLFGDMGAATPYTTFVRTQDESIST+RWILRDI
Sbjct: 258 QVGSESKGWSSIQSFMSRNEDSDETIAFLFGDMGAATPYTTFVRTQDESISTVRWILRDI 317

Query: 309 EALGDKPAFVSHIGDISYARGHAWLWDVFFNQIEPVASKVAYHVCIGNHEYDWPLQPWKP 368
           EALGDKPAFVSHIGDISYARGH+WLWDVFFNQIEPVASKVAYHVCIGNHEYDWPLQPWKP
Sbjct: 318 EALGDKPAFVSHIGDISYARGHSWLWDVFFNQIEPVASKVAYHVCIGNHEYDWPLQPWKP 377

Query: 369 EWAYSIYGKDGGGECGVPYSLKFNMPGNSSEPTAAHSLPTRNLFYSFNMGSVHFVYISTE 428
           +WA  IYGKDGGGECGVPYSLKFNMPGNSSEPT A+ +PTRNLFYSFNMGSVHFVYISTE
Sbjct: 378 DWASGIYGKDGGGECGVPYSLKFNMPGNSSEPTKANGVPTRNLFYSFNMGSVHFVYISTE 437

Query: 429 TNFLQGSRQYEFIKLDLESVDRKKTPFVVVQGHRPMYTTSNELRDTPLREKMLQHLEPLF 488
           TNFL+GSRQ+ F++ DLESVDRKKTPFVVVQGHRPMYTTSNELRDTPLREKML HLEPLF
Sbjct: 438 TNFLKGSRQFSFLERDLESVDRKKTPFVVVQGHRPMYTTSNELRDTPLREKMLHHLEPLF 497

Query: 489 VKNNVTLALWGHVHRYERFCPLNNYTCGSMSVDGEEWEALPVHLVIGMAGQDWQPIWEPR 548
           VKNNVTLALWGHVHRYERFCPL NYTCGSM VDGE+WEALPVHLVIGMAGQDWQPIWEPR
Sbjct: 498 VKNNVTLALWGHVHRYERFCPLKNYTCGSMGVDGEDWEALPVHLVIGMAGQDWQPIWEPR 557

Query: 549 PNHPDDPIFPQPKRSMYRGGEFGYTRLVATKEKLTVSYVGNHDGEVHDSVEILASGQVLN 608
           PNH D PIFPQPKRSMYRGGEFGYTRLVATKEKLT+SYVGNHDGEVHDSVEILASGQV+N
Sbjct: 558 PNHLDVPIFPQPKRSMYRGGEFGYTRLVATKEKLTISYVGNHDGEVHDSVEILASGQVVN 617

Query: 609 SGIDAKFINSSLENSRTRNEMLESSFSWYVMGASILVLGAFIGYIIGFVSHARKNSISRN 668
            G+ AKF N S+ENSR+RN MLESSFSWYVMG SILVLGAFIGYI+GFVSHARK   SRN
Sbjct: 618 GGVGAKF-NESVENSRSRNGMLESSFSWYVMGGSILVLGAFIGYIVGFVSHARK---SRN 677

Query: 669 SWTPVKTEELQ 679
           +WTPVKTEELQ
Sbjct: 678 NWTPVKTEELQ 684

BLAST of Lag0003992 vs. ExPASy Swiss-Prot
Match: Q9LMG7 (Probable inactive purple acid phosphatase 2 OS=Arabidopsis thaliana OX=3702 GN=PAP2 PE=2 SV=1)

HSP 1 Score: 957.2 bits (2473), Expect = 9.8e-278
Identity = 451/654 (68.96%), Postives = 534/654 (81.65%), Query Frame = 0

Query: 27  LILLISFLPFSTQSKVSISFSPSTLSKSGDPVQIQWSGIESPSNLDWLGIYSPPNSSHKH 86
           L+L +S    S  SK +IS SP+ L++SGD V IQWSG++SPS+LDWLG+YSPP S + H
Sbjct: 9   LLLFVSVFVSSADSKATISISPNALNRSGDSVVIQWSGVDSPSDLDWLGLYSPPESPNDH 68

Query: 87  FIGYVFLSSSPGWESGFGSVSIPLVNLRSNYSFRIFRWTESEIDEKHQDHDHNPLPGTAH 146
           FIGY FL+ S  W+ GFGS+S+PL NLRSNY+FRIFRW+ESEID KH+DHD NPLPGT H
Sbjct: 69  FIGYKFLNESSTWKDGFGSISLPLTNLRSNYTFRIFRWSESEIDPKHKDHDQNPLPGTKH 128

Query: 147 LLAASEELQFSPG-GGPEQIHLAFTDHDDEMRVMFVTEDGSERYVRYGE-REKLDQIVAA 206
           LLA SE+L F  G G PEQIHL+FT+  + MRVMFV  DG ER+VRYGE ++ L    AA
Sbjct: 129 LLAESEQLTFGSGVGMPEQIHLSFTNMVNTMRVMFVAGDGEERFVRYGESKDLLGNSAAA 188

Query: 207 RVQRYEREHMCDSPANDSIGWRDPGFIHDAVMKKLQKGARIYYQVGSDSKGWSSIRSFVS 266
           R  RYEREHMCDSPAN +IGWRDPG+I D VMK L  G R YYQVGSDSKGWS I S+++
Sbjct: 189 RGMRYEREHMCDSPANSTIGWRDPGWIFDTVMKNLNDGVRYYYQVGSDSKGWSEIHSYIA 248

Query: 267 RNEDLDETIAFLFGDMGAATPYTTFVRTQDESISTIRWILRDIEALGDKPAFVSHIGDIS 326
           R+   +ET+AF+FGDMG ATPYTTF+RTQDESIST++WILRDIEALGDKPA +SHIGDIS
Sbjct: 249 RDVTAEETVAFMFGDMGCATPYTTFIRTQDESISTVKWILRDIEALGDKPAMISHIGDIS 308

Query: 327 YARGHAWLWDVFFNQIEPVASKVAYHVCIGNHEYDWPLQPWKPEWAYSIYGKDGGGECGV 386
           YARG++W+WD FF Q+EP+AS V YHVCIGNHEYD+  QPWKP+WA SIYG DGGGECGV
Sbjct: 309 YARGYSWVWDEFFAQVEPIASTVPYHVCIGNHEYDFSTQPWKPDWAASIYGNDGGGECGV 368

Query: 387 PYSLKFNMPGNSSEPTAAHSLPTRNLFYSFNMGSVHFVYISTETNFLQGSRQYEFIKLDL 446
           PYSLKFNMPGNSSE T   + PTRNL+YS++MG+VHFVYISTETNFL+G  QYEFIK DL
Sbjct: 369 PYSLKFNMPGNSSESTGMKAPPTRNLYYSYDMGTVHFVYISTETNFLKGGSQYEFIKRDL 428

Query: 447 ESVDRKKTPFVVVQGHRPMYTTSNELRDTPLREKMLQHLEPLFVKNNVTLALWGHVHRYE 506
           ESVDRKKTPFVVVQGHRPMYTTSNE+RDT +R+KM++HLEPLFVKNNVTLALWGHVHRYE
Sbjct: 429 ESVDRKKTPFVVVQGHRPMYTTSNEVRDTMIRQKMVEHLEPLFVKNNVTLALWGHVHRYE 488

Query: 507 RFCPLNNYTCGSMSVDGEEWEALPVHLVIGMAGQDWQPIWEPRPNHPDDPIFPQPKRSMY 566
           RFCP++N TCG+      +W+  PVHLVIGMAGQDWQPIW+PRPNHPD PIFPQP++SMY
Sbjct: 489 RFCPISNNTCGT------QWQGNPVHLVIGMAGQDWQPIWQPRPNHPDLPIFPQPEQSMY 548

Query: 567 RGGEFGYTRLVATKEKLTVSYVGNHDGEVHDSVEILASGQVLNSGIDAKFINS--SLENS 626
           R GEFGYTRLVA KEKLTVS+VGNHDGEVHD+VE+LASG V++   ++  I +  ++  S
Sbjct: 549 RTGEFGYTRLVANKEKLTVSFVGNHDGEVHDTVEMLASGVVISGSKESTKIPNLKTVPAS 608

Query: 627 RTRNEMLESSFSWYVMGASILVLGAFIGYIIGFVSHARKNSISRNSWTPVKTEE 677
            T     ES+  WY  GA ++V+G  +G+IIGF +  +K+S S N W PVK EE
Sbjct: 609 ATLMGKSESNALWYAKGAGLMVVGVLLGFIIGFFTRGKKSS-SGNRWIPVKNEE 655

BLAST of Lag0003992 vs. ExPASy Swiss-Prot
Match: Q9ZQ81 (Probable inactive purple acid phosphatase 9 OS=Arabidopsis thaliana OX=3702 GN=PAP9 PE=2 SV=1)

HSP 1 Score: 909.1 bits (2348), Expect = 3.1e-263
Identity = 438/657 (66.67%), Postives = 517/657 (78.69%), Query Frame = 0

Query: 29  LLISFLPFSTQSKVSISFSPSTLSKSGDPVQIQWSGIESPSNLDWLGIYSPPNSSHKHFI 88
           LLIS    S  SK +IS SP TL++SGD V I+WSG+ESPS+LDWLGIYSPP+S H HFI
Sbjct: 13  LLIS----SVYSKATISISPQTLNRSGDIVVIKWSGVESPSDLDWLGIYSPPDSPHDHFI 72

Query: 89  GYVFLSSSPGWESGFGSVSIPLVNLRSNYSFRIFRWTESEIDEKHQDHDHNPLPGTAHLL 148
           GY FLS SP W+SG GS+S+PL NLRSNY+FRIF WT+SEI+ KHQDHDHNPLPGT HLL
Sbjct: 73  GYKFLSDSPTWQSGSGSISLPLTNLRSNYTFRIFHWTQSEINPKHQDHDHNPLPGTRHLL 132

Query: 149 AASEELQFS-PGGGPEQIHLAFTDHDDEMRVMFVTEDGSERYVRYGE-REKLDQIVAARV 208
             S +L F      PEQIHL++TD+ +EMRV+FVT DG ER  RYGE ++KLD I  AR 
Sbjct: 133 TESNQLNFRFAVNRPEQIHLSYTDNINEMRVVFVTGDGEEREARYGEVKDKLDNIAVARG 192

Query: 209 QRYEREHMCDSPANDSIGWRDPGFIHDAVMKKLQKGARIYYQVGSDSKGWSSIRSFVSRN 268
            RYE EHMC +PAN ++GWRDPG+  DAVMK L++G R YYQVGSD KGWS I SFVSRN
Sbjct: 193 VRYEIEHMCHAPANSTVGWRDPGWTFDAVMKNLKQGIRYYYQVGSDLKGWSEIHSFVSRN 252

Query: 269 EDLDETIAFLFGDMGAATPYTTFVRTQDESISTIRWILRDIEALG-DKPAFVSHIGDISY 328
           E  +ET+AF+FGDMG  TPYTTF+R ++ES+ST++WILRDIEALG DKP  VSHIGDISY
Sbjct: 253 EGSEETLAFMFGDMGCYTPYTTFIRGEEESLSTVKWILRDIEALGDDKPVIVSHIGDISY 312

Query: 329 ARGHAWLWDVFFNQIEPVASKVAYHVCIGNHEYDWPLQPWKPEWAYSIYGKDGGGECGVP 388
           ARG++W+WD FF QIEP+ASKV YHVCIGNHEYDWP QPWKP+WA  +YGKD GGECGVP
Sbjct: 313 ARGYSWIWDEFFTQIEPIASKVPYHVCIGNHEYDWPNQPWKPDWAAYVYGKDSGGECGVP 372

Query: 389 YSLKFNMPGNSSEPTAAHSLP-TRNLFYSFNMGSVHFVYISTETNFLQGSRQYEFIKLDL 448
           YS+KFNMPGNS+E T     P +RNL+YS++MGSVHFVYISTET+FL+G +QY F+K DL
Sbjct: 373 YSVKFNMPGNSTEATGMVKGPQSRNLYYSYDMGSVHFVYISTETDFLKGGKQYSFLKSDL 432

Query: 449 ESVDRKKTPFVVVQGHRPMYTTSNELRDTPLREKMLQHLEPLFVKNNVTLALWGHVHRYE 508
           ESV+R KTPFVVVQGHRPMYTTS ++RD  +REKM++HLEPL VKNNVT+ALWGHVHRYE
Sbjct: 433 ESVNRSKTPFVVVQGHRPMYTTSRKIRDAAIREKMIEHLEPLLVKNNVTVALWGHVHRYE 492

Query: 509 RFCPLNNYTCGSMSVDGEEWEALPVHLVIGMAGQDWQPIWEPRPNHPDDPIFPQPKRSMY 568
           RFC ++N TC      GE W+  PVHLVIGMAG+D QP+WEPR NH D PIFPQP  SMY
Sbjct: 493 RFCAISNNTC------GERWQGNPVHLVIGMAGKDSQPMWEPRANHEDVPIFPQPANSMY 552

Query: 569 RGGEFGYTRLVATKEKLTVSYVGNHDGEVHDSVEILASGQVLNSGIDAKFINSSLENSRT 628
           RGGEFGY RLVA KE+LT+SYVGNHDGEVHD VEILASG+V         I+ S + ++ 
Sbjct: 553 RGGEFGYIRLVANKERLTLSYVGNHDGEVHDVVEILASGEV---------ISGSDDGTKD 612

Query: 629 RNEMLESSFS--WYVMGASILVLGAFIGYIIGFVSHARKNS---ISRNSWTPVKTEE 677
            N   ES F+  WY+ GAS++V+G   GY +GF+S  +K S    S  SW  VK EE
Sbjct: 613 SNFGSESDFAVLWYIEGASVMVVGVIFGYFVGFLSRKKKESGVGSSNRSWIQVKNEE 650

BLAST of Lag0003992 vs. ExPASy Swiss-Prot
Match: Q9LMX4 (Probable inactive purple acid phosphatase 1 OS=Arabidopsis thaliana OX=3702 GN=PAP1 PE=2 SV=1)

HSP 1 Score: 229.6 bits (584), Expect = 1.1e-58
Identity = 181/607 (29.82%), Postives = 277/607 (45.63%), Query Frame = 0

Query: 34  LPFSTQSKVSISFSPSTLSKSG---DPVQIQWSGIESPSNLDWLGIYSPPNSSHKHFIG- 93
           + F    K  +  SP+ L  +G   + V +Q+S    PS+ DW+G++SP + +     G 
Sbjct: 38  ITFGLNEKAYVKASPTVLGSNGQHSELVLVQYSS-PKPSDDDWIGVFSPADFNASTCPGD 97

Query: 94  -----------------YVFLSSSPGWESGFGSVSIPLVNLRSNYSFRIFRWTESEIDEK 153
                            Y   S+     +G GS+ + L+N RS++SF +F  +   ++ K
Sbjct: 98  NKMVQPPRLCSAPVKFQYANFSNPRYTNTGTGSLKLQLINQRSDFSFALF--SGGLLNPK 157

Query: 154 HQDHDHNPLPGTAHLLAASEELQFSPGGGPEQIHLAFTDHDDEMRVMFVTEDG---SERY 213
                         L+A S ++ F     P    LA     DEM V + +  G   +E  
Sbjct: 158 --------------LVAISNKVAFENPNAPVYPRLALGKEWDEMTVTWTSGYGLNLAEPV 217

Query: 214 VRYGEREKLDQIVAARVQRYEREHMCDSPANDSIGWRDPGFIHDAVMKKLQKGARIYYQV 273
           V +G +    ++  A    + R  MC +PA  ++GWRDPG+IH A +K+L   ++  Y+V
Sbjct: 218 VEWGVKGGERKLSPAGTLTFARNSMCGAPAR-TVGWRDPGYIHTAFLKELWPNSKYTYRV 277

Query: 274 GSD-SKG---WSSIRSFVSRNEDLDETI--AFLFGDMGAA-----TPYTTFVRTQDESIS 333
           G   S G   WS    F S       ++    +FGDMG A     + Y  F R    S++
Sbjct: 278 GHRLSNGALIWSKEYQFKSSPFPGQNSVQQVVIFGDMGKAEVDGSSEYNDFQRA---SLN 337

Query: 334 TIRWILRDIEALGDKPAFVSHIGDISYARGHAWLWDVFFNQIEPVASKVAYHVCIGNHEY 393
           T + +++D++    K   V HIGDI YA G+   WD F  QIEP+AS V Y +  GNHE 
Sbjct: 338 TTKQLIKDLK----KTDAVFHIGDICYANGYLSQWDQFIAQIEPIASTVPYMIASGNHER 397

Query: 394 DWPLQPWKPEWAYSIY-GKDGGGECGVPYSLKFNMPGNSSEPTAAHSLPTRNLFYSFNMG 453
            WP        + S Y G D GGECGVP    F +P  +             ++YS + G
Sbjct: 398 VWPN-------SGSFYEGLDSGGECGVPAETMFYVPAQN----------RAKVWYSSDYG 457

Query: 454 SVHFVYISTETNFLQGSRQYEFIKLDLESVDRKKTPFVVVQGHRPMYTTSNEL--RDTPL 513
              F    TE ++ +G+ QY FI+  L SVDR+K P+++   HR +  +S      +   
Sbjct: 458 MFRFCVADTEHDWREGTEQYNFIEHCLASVDRQKQPWLIFLAHRVLGYSSTYFYAEEGSF 517

Query: 514 REKM-LQHLEPLFVKNNVTLALWGHVHRYERFCPLNNYTCGSMSVDGEEWEAL-PVHLVI 573
            E M  + L+ L+ K  V +A++GH H YER CP+    C S      +      +H+V 
Sbjct: 518 AEPMGRESLQKLWQKYKVDIAIYGHAHNYERTCPVYQSVCTSHEKSNYKAPLNGTIHIVA 577

Query: 574 GMAGQDWQPIWEPRPNHPDDPIFPQPKRSMYRGGEFGYTRLVA-TKEKLTVSYVGNHDGE 600
           G  G       + +PN            S++R  ++G+ +L A     L   Y  + DG 
Sbjct: 578 GGGGAGLAEFSDLQPNW-----------SLFRDYDYGFLKLTAIDHSNLLFEYKKSSDGR 591

BLAST of Lag0003992 vs. ExPASy Swiss-Prot
Match: Q5MAU8 (Probable inactive purple acid phosphatase 27 OS=Arabidopsis thaliana OX=3702 GN=PAP27 PE=2 SV=1)

HSP 1 Score: 222.2 bits (565), Expect = 1.7e-56
Identity = 175/599 (29.22%), Postives = 273/599 (45.58%), Query Frame = 0

Query: 44  ISFSPSTLSKSGDPVQIQWSGI----ESPSNLDWLGIYSPPN--------SSHKHF---- 103
           I  SP  L   G     +W  +      PS+ DW+G++SP          +  K      
Sbjct: 47  IHVSPLVLGSQGQ--DTEWVNVVISNPEPSSDDWVGVFSPAKFDSSSCAPTDDKEIAPFI 106

Query: 104 ----IGYVFLSSSPGW-ESGFGSVSIPLVNLRSNYSFRIFRWTESEIDEKHQDHDHNPLP 163
               + Y++  SSP + ++G   +   L+N R+++SF +F    S           NP  
Sbjct: 107 CSAPVKYMYAKSSPDYMKTGNAVLKFMLINQRADFSFALFTGGLS-----------NPT- 166

Query: 164 GTAHLLAASEELQFSPGGGPEQIHLAFTDHDDEMRVMFVTE---DGSERYVRYGEREKLD 223
               L++ S  + F     P    LA     DEM V + +      +  +V +  +    
Sbjct: 167 ----LVSVSNHVSFINPKAPVYPRLALGKKWDEMTVTWTSGYNIGEAVPFVEWSRKGTRS 226

Query: 224 QIVAARVQRYEREHMCDSPANDSIGWRDPGFIHDAVMKKLQKGARIYYQVGSD----SKG 283
           +   A    + R  MC +PA  ++GWRDPGFIH A +K L    +  Y++G +    S  
Sbjct: 227 RRSPAGTLTFTRNSMCGAPAR-TVGWRDPGFIHTASLKDLWPNLKYTYRMGHELMNGSIV 286

Query: 284 WSSIRSFVSRNEDLDETI--AFLFGDMG-----AATPYTTFVRTQDESISTIRWILRDIE 343
           WS   +F S      +++    +FGDMG      +  Y  +   Q  S++T   +++D++
Sbjct: 287 WSKNFTFKSSPYPGQDSLQRVIIFGDMGKGERDGSNEYNDY---QPGSLNTTDQLIKDLK 346

Query: 344 ALGDKPAFVSHIGDISYARGHAWLWDVFFNQIEPVASKVAYHVCIGNHEYDWPLQPWKPE 403
            +      V HIGDI+YA G+   WD F  Q+EP+AS V Y V  GNHE DWP       
Sbjct: 347 NID----IVFHIGDITYANGYISQWDQFTAQVEPIASTVPYMVASGNHERDWP------- 406

Query: 404 WAYSIY-GKDGGGECGVPYSLKFNMPGNSSEPTAAHSLPTRNLFYSFNMGSVHFVYISTE 463
            + S Y GKD GGECGVP    F+ P  +              +YS + G   F    TE
Sbjct: 407 DSGSFYGGKDSGGECGVPAETMFDFPAENK----------AKFWYSADYGMFRFCVADTE 466

Query: 464 TNFLQGSRQYEFIKLDLESVDRKKTPFVVVQGHRPMYTTSNEL--RDTPLREKM-LQHLE 523
            ++ +GS QY+FI+  L SVDR+  P+++   HR +  ++N+   ++    E M  + L+
Sbjct: 467 HDWREGSEQYQFIERCLASVDRRAQPWLIFIAHRVLGYSTNDWYGQEGSFEEPMGRESLQ 526

Query: 524 PLFVKNNVTLALWGHVHRYERFCPLNNYTCGSMSVDGEEWEAL---PVHLVIGMAGQDWQ 583
            L+ K  V +A +GHVH YER CP+    C  M  +   +       +H+V+G AG    
Sbjct: 527 KLWQKYKVDIAFYGHVHNYERTCPIYQNQC--MDNEKSHYSGAFKGTIHVVVGGAG---- 586

Query: 584 PIWEPRPNHPDDPIFPQPKRSMYRGGEFGYTRLVA-TKEKLTVSYVGNHDGEVHDSVEI 600
                  +H       +PK S++R  ++G+ +L A     L   Y  + +G VHDS  I
Sbjct: 587 -------SHLSSFSSLKPKWSIFRDYDYGFVKLTAFDHSSLLFEYKKSSNGAVHDSFTI 589

BLAST of Lag0003992 vs. ExPASy Swiss-Prot
Match: Q8H1R2 (Probable inactive purple acid phosphatase 24 OS=Arabidopsis thaliana OX=3702 GN=PAP24 PE=2 SV=1)

HSP 1 Score: 219.9 bits (559), Expect = 8.6e-56
Identity = 169/604 (27.98%), Postives = 274/604 (45.36%), Query Frame = 0

Query: 40  SKVSISFSPSTLSKSGDPVQIQWSGI----ESPSNLDWLGIYSP---------PNSSHKH 99
           S V +  SP  L   G+    +W  +      P++ DW+G++SP         P S  K 
Sbjct: 46  SSVKLHASPQVLGSQGE--DTEWVNLAISNPKPTSDDWIGVFSPAKFDSGNCWPTSGGKE 105

Query: 100 F--------IGYVFLSSSPGW-ESGFGSVSIPLVNLRSNYSFRIFRWTESEIDEKHQDHD 159
                    I Y++ +S P + +SG  ++   ++N R++ SF +F    + + E      
Sbjct: 106 KTPYICSSPIKYMYCNSHPDYMKSGNVTLKFQIINQRADVSFALF---SNGVQE------ 165

Query: 160 HNPLPGTAHLLAASEELQFSPGGGPEQIHLAFTDHDDEMRVMFVTE---DGSERYVRYGE 219
                   HLL  S  + F     P    LA   + DEM V + +    D +  ++ +  
Sbjct: 166 -------PHLLGVSNPVAFFNPKAPVYPRLALGKNWDEMTVTWTSGYNIDEAVPFIEWSA 225

Query: 220 REKLDQIVAARVQRYEREHMCDSPANDSIGWRDPGFIHDAVMKKLQKGARIYYQVGSD-- 279
           +    +   A    + R  MC +PA   +GWRDPGF H + +K+L       Y++G D  
Sbjct: 226 KGLPARRSPAGTLTFNRNSMCGNPAR-GVGWRDPGFFHTSFLKELWPNREYIYRLGHDLV 285

Query: 280 --SKGWSSIRSFVSR---NEDLDETIAFLFGDMG-----AATPYTTFVRTQDESISTIRW 339
             S  WS   +FVS     +D  + +  +FGDMG      +  Y  +   Q  S++T   
Sbjct: 286 NGSTIWSKNYTFVSSPYPGQDSKQRV-IIFGDMGKGERDGSNEYNDY---QPGSLNTTDQ 345

Query: 340 ILRDIEALGDKPAFVSHIGDISYARGHAWLWDVFFNQIEPVASKVAYHVCIGNHEYDWPL 399
           +++D++ +      V HIGD++Y+ G+   WD F  Q++P+AS V Y +  GNHE DW  
Sbjct: 346 VIKDLKDID----IVFHIGDLTYSNGYLSQWDQFTAQVQPIASTVPYMIASGNHERDW-- 405

Query: 400 QPWKPEWAYSIYGKDGGGECGVPYSLKFNMPGNSSEPTAAHSLPTRNLFYSFNMGSVHFV 459
               P+      G D GGECGVP    F  P  +              +Y  + G   F 
Sbjct: 406 ----PDTGSFYAGTDSGGECGVPAETMFYFPAEN----------RAKFWYKTDYGMFRFC 465

Query: 460 YISTETNFLQGSRQYEFIKLDLESVDRKKTPFVVVQGHRPMYTTSNEL--RDTPLREKM- 519
              +E ++ +G+ QY+FI+  L +VDRK  P+++   HR +  ++N+   ++    E M 
Sbjct: 466 VADSEHDWREGTEQYKFIENCLATVDRKTQPWLIFIAHRVLGYSTNDWYGKEGTFEEPMG 525

Query: 520 LQHLEPLFVKNNVTLALWGHVHRYERFCPLNNYTCGSMSVDGEEWEAL---PVHLVIGMA 579
            + L+ L+ K  V LA +GHVH YER CP+    C  ++ D + +       +H+V+G A
Sbjct: 526 RESLQKLWQKYKVDLAFYGHVHNYERTCPIYESQC--VNNDKDHYSGTFKGTIHVVVGGA 585

Query: 580 GQDWQPIWEPRPNHPDDPIFPQPKRSMYRGGEFGYTRLVAT-KEKLTVSYVGNHDGEVHD 600
           G    P                PK S+ R  +FG+ +L A+    L   Y  +  G+V+D
Sbjct: 586 GSHLSPFSS-----------LVPKWSLVRDYDFGFVKLTASDHSSLLFEYKKSSTGQVYD 593

BLAST of Lag0003992 vs. ExPASy TrEMBL
Match: A0A1S3CPV0 (Purple acid phosphatase OS=Cucumis melo OX=3656 GN=LOC103503324 PE=3 SV=1)

HSP 1 Score: 1256.1 bits (3249), Expect = 0.0e+00
Identity = 595/656 (90.70%), Postives = 620/656 (94.51%), Query Frame = 0

Query: 24  LIPLILLISFLPFSTQSKVSISFSPSTLSKSGDPVQIQWSGIESPSNLDWLGIYSPPNSS 83
           +IP ILL SF+P S QSKVSIS SPS LSKSGD V IQWSGIESPSNLDWLGIYSPPNSS
Sbjct: 5   IIPFILLFSFVPLSFQSKVSISLSPSILSKSGDSVHIQWSGIESPSNLDWLGIYSPPNSS 64

Query: 84  HKHFIGYVFLSSSPGWESGFGSVSIPLVNLRSNYSFRIFRWTESEIDEKHQDHDHNPLPG 143
           HKHF+GY+FLSSSP WESG+GSVSIPLVNLRSNYSFRIFRWTESEID+KH DHDHNPLPG
Sbjct: 65  HKHFVGYLFLSSSPTWESGYGSVSIPLVNLRSNYSFRIFRWTESEIDDKHHDHDHNPLPG 124

Query: 144 TAHLLAASEELQFSPGGGPEQIHLAFTDHDDEMRVMFVTEDGSERYVRYGE-REKLDQIV 203
           TAHLLAAS+EL+F+PGGGPEQIHLAFTD DDEMRVMFVTEDGSERYVRYGE +EKLDQIV
Sbjct: 125 TAHLLAASDELRFAPGGGPEQIHLAFTDQDDEMRVMFVTEDGSERYVRYGEKKEKLDQIV 184

Query: 204 AARVQRYEREHMCDSPANDSIGWRDPGFIHDAVMKKLQKGARIYYQVGSDSKGWSSIRSF 263
            A V+RYEREHMCDSPANDSIGWRDPGFIHDAVM KL+KGA++YYQVGSDSKGWSSI +F
Sbjct: 185 VAGVERYEREHMCDSPANDSIGWRDPGFIHDAVMNKLKKGAKVYYQVGSDSKGWSSILNF 244

Query: 264 VSRNEDLDETIAFLFGDMGAATPYTTFVRTQDESISTIRWILRDIEALGDKPAFVSHIGD 323
           VSRNED DETIAFLFGDMGAATPYTTFVRTQDESIST+RWILRDIEALGDKPA VSHIGD
Sbjct: 245 VSRNEDSDETIAFLFGDMGAATPYTTFVRTQDESISTVRWILRDIEALGDKPAMVSHIGD 304

Query: 324 ISYARGHAWLWDVFFNQIEPVASKVAYHVCIGNHEYDWPLQPWKPEWAYSIYGKDGGGEC 383
           ISYARGH+WLWDVFFNQIEPVASKVAYHVCIGNHEYDWPLQPWKPEWAY IYGKDGGGEC
Sbjct: 305 ISYARGHSWLWDVFFNQIEPVASKVAYHVCIGNHEYDWPLQPWKPEWAYGIYGKDGGGEC 364

Query: 384 GVPYSLKFNMPGNSSEPTAAHSLPTRNLFYSFNMGSVHFVYISTETNFLQGSRQYEFIKL 443
           GVPYSLKFNMPGN SEPT +HSLPTRNLFYSFNMGSVHFVYISTETNFLQGS QYEFIK 
Sbjct: 365 GVPYSLKFNMPGNFSEPTESHSLPTRNLFYSFNMGSVHFVYISTETNFLQGSSQYEFIKR 424

Query: 444 DLESVDRKKTPFVVVQGHRPMYTTSNELRDTPLREKMLQHLEPLFVKNNVTLALWGHVHR 503
           DLESVDRKKTPFVVVQGHRPMYTTSNELRD PLREKML HLEPL VKNNVTLALWGHVHR
Sbjct: 425 DLESVDRKKTPFVVVQGHRPMYTTSNELRDAPLREKMLHHLEPLLVKNNVTLALWGHVHR 484

Query: 504 YERFCPLNNYTCGSMSVDGEEWEALPVHLVIGMAGQDWQPIWEPRPNHPDDPIFPQPKRS 563
           YERFCPLNNYTCGSM +DGE+WEALPVHLVIGMAGQDWQPIWEPRPNHPDDPIFPQPKRS
Sbjct: 485 YERFCPLNNYTCGSMGLDGEDWEALPVHLVIGMAGQDWQPIWEPRPNHPDDPIFPQPKRS 544

Query: 564 MYRGGEFGYTRLVATKEKLTVSYVGNHDGEVHDSVEILASGQVLNSGIDAKFINSSLENS 623
           MYRGGEFGYTRLVATKEKLT+SYVGNHDGEVHDSVEILASGQVLN  + AKFI+SS+ NS
Sbjct: 545 MYRGGEFGYTRLVATKEKLTISYVGNHDGEVHDSVEILASGQVLNGNVGAKFIDSSIANS 604

Query: 624 RTRNEMLESSFSWYVMGASILVLGAFIGYIIGFVSHARKNSISRNSWTPVKTEELQ 679
            T N MLE SFSWYVMG SILVLGAFIGYIIGFVSHARKNSISRN+WTPVKTEELQ
Sbjct: 605 TTGNAMLEFSFSWYVMGGSILVLGAFIGYIIGFVSHARKNSISRNNWTPVKTEELQ 660

BLAST of Lag0003992 vs. ExPASy TrEMBL
Match: A0A0A0KNH8 (Purple acid phosphatase OS=Cucumis sativus OX=3659 GN=Csa_5G487720 PE=3 SV=1)

HSP 1 Score: 1252.3 bits (3239), Expect = 0.0e+00
Identity = 589/656 (89.79%), Postives = 621/656 (94.66%), Query Frame = 0

Query: 24  LIPLILLISFLPFSTQSKVSISFSPSTLSKSGDPVQIQWSGIESPSNLDWLGIYSPPNSS 83
           +IP +LL SF+P S QSKVS+SFSPS LSKSGD V IQWSGIESPS LDWLGIYSPPNSS
Sbjct: 5   IIPFVLLFSFIPISFQSKVSVSFSPSILSKSGDSVHIQWSGIESPSKLDWLGIYSPPNSS 64

Query: 84  HKHFIGYVFLSSSPGWESGFGSVSIPLVNLRSNYSFRIFRWTESEIDEKHQDHDHNPLPG 143
           HKHFIGY+FLSSSP WESG+GSVSIPLVNLRSNY+FRIFRWTESEID+KH DHDHNPLPG
Sbjct: 65  HKHFIGYLFLSSSPTWESGYGSVSIPLVNLRSNYAFRIFRWTESEIDDKHHDHDHNPLPG 124

Query: 144 TAHLLAASEELQFSPGGGPEQIHLAFTDHDDEMRVMFVTEDGSERYVRYGE-REKLDQIV 203
           TAHLLAAS+EL+F+PGGGPEQIHLAFTD DDEMRVMFVT+DGS+RYVRYGE +EKLDQIV
Sbjct: 125 TAHLLAASDELRFAPGGGPEQIHLAFTDQDDEMRVMFVTKDGSKRYVRYGEKKEKLDQIV 184

Query: 204 AARVQRYEREHMCDSPANDSIGWRDPGFIHDAVMKKLQKGARIYYQVGSDSKGWSSIRSF 263
            A V+RYEREHMCDSPANDSIGWRDPGFIHDAVM KL+KGA++YYQVGSDSKGWSSI +F
Sbjct: 185 VAGVERYEREHMCDSPANDSIGWRDPGFIHDAVMNKLKKGAKVYYQVGSDSKGWSSILNF 244

Query: 264 VSRNEDLDETIAFLFGDMGAATPYTTFVRTQDESISTIRWILRDIEALGDKPAFVSHIGD 323
           VSRNED DETIAFLFGDMGAATPYTTFVRTQDESIST+RWILRDIEALGDKPA VSHIGD
Sbjct: 245 VSRNEDSDETIAFLFGDMGAATPYTTFVRTQDESISTVRWILRDIEALGDKPAMVSHIGD 304

Query: 324 ISYARGHAWLWDVFFNQIEPVASKVAYHVCIGNHEYDWPLQPWKPEWAYSIYGKDGGGEC 383
           ISYARGH+WLWDVFFNQ+EPVASKVAYHVCIGNHEYDWPLQPWKPEWA  IYGKDGGGEC
Sbjct: 305 ISYARGHSWLWDVFFNQVEPVASKVAYHVCIGNHEYDWPLQPWKPEWANGIYGKDGGGEC 364

Query: 384 GVPYSLKFNMPGNSSEPTAAHSLPTRNLFYSFNMGSVHFVYISTETNFLQGSRQYEFIKL 443
           GVPYSLKFNMPGNS+EPT +HSLPTRNLFYSFNMGSVHFVYISTETNFLQGS QYEFIK 
Sbjct: 365 GVPYSLKFNMPGNSTEPTESHSLPTRNLFYSFNMGSVHFVYISTETNFLQGSSQYEFIKR 424

Query: 444 DLESVDRKKTPFVVVQGHRPMYTTSNELRDTPLREKMLQHLEPLFVKNNVTLALWGHVHR 503
           DLESVDRKKTPF+VVQGHRPMYTTSNELRD PLREKML HLEPL VKNNVTLALWGHVHR
Sbjct: 425 DLESVDRKKTPFIVVQGHRPMYTTSNELRDAPLREKMLHHLEPLLVKNNVTLALWGHVHR 484

Query: 504 YERFCPLNNYTCGSMSVDGEEWEALPVHLVIGMAGQDWQPIWEPRPNHPDDPIFPQPKRS 563
           YERFCPLNNYTCGSM +DGE+WEALPVHLVIGMAGQDWQPIWEPRPNHPDDPIFPQPKRS
Sbjct: 485 YERFCPLNNYTCGSMGLDGEDWEALPVHLVIGMAGQDWQPIWEPRPNHPDDPIFPQPKRS 544

Query: 564 MYRGGEFGYTRLVATKEKLTVSYVGNHDGEVHDSVEILASGQVLNSGIDAKFINSSLENS 623
           MYRGGEFGYTRLVATKEKLT+SYVGNHDGEVHDSVEILASGQVLN G+ AKFINSS+ NS
Sbjct: 545 MYRGGEFGYTRLVATKEKLTISYVGNHDGEVHDSVEILASGQVLNGGVGAKFINSSIANS 604

Query: 624 RTRNEMLESSFSWYVMGASILVLGAFIGYIIGFVSHARKNSISRNSWTPVKTEELQ 679
            T N MLE SFSWYVMG SILVLGAFIGYIIGFVSHARKNS+SRN+WTPVKTEELQ
Sbjct: 605 TTGNAMLEFSFSWYVMGGSILVLGAFIGYIIGFVSHARKNSLSRNNWTPVKTEELQ 660

BLAST of Lag0003992 vs. ExPASy TrEMBL
Match: A0A6J1IE32 (Purple acid phosphatase OS=Cucurbita maxima OX=3661 GN=LOC111471840 PE=3 SV=1)

HSP 1 Score: 1242.6 bits (3214), Expect = 0.0e+00
Identity = 596/671 (88.82%), Postives = 627/671 (93.44%), Query Frame = 0

Query: 9   SSIPITCSQSIPMNNLIPLILLISFLPFSTQSKVSISFSPSTLSKSGDPVQIQWSGIESP 68
           SS+  TCS  IPM NLI LILLIS  P S QSKVSIS SPSTL+KSGDPVQIQWSG+ESP
Sbjct: 18  SSVFDTCSSFIPMENLIYLILLISIFPISIQSKVSISISPSTLNKSGDPVQIQWSGVESP 77

Query: 69  SNLDWLGIYSPPNSSHKHFIGYVFLSSSPGWESGFGSVSIPLVNLRSNYSFRIFRWTESE 128
           SNLDWLGIYSPPNSSHKHFIGYVFLSSSPGWESG GSVSIPLVNLRS YSFRIFRWTESE
Sbjct: 78  SNLDWLGIYSPPNSSHKHFIGYVFLSSSPGWESGSGSVSIPLVNLRSKYSFRIFRWTESE 137

Query: 129 IDEKHQDHDHNPLPGTAHLLAASEELQFSPGGGPEQIHLAFTDHDDEMRVMFVTEDGSER 188
           ID+KH DHD+NPLPGT HLLAASEEL+FSPG GPEQIHLAFTD DDEMRVMFVTEDGSER
Sbjct: 138 IDDKHHDHDNNPLPGTKHLLAASEELRFSPGRGPEQIHLAFTDQDDEMRVMFVTEDGSER 197

Query: 189 YVRYGER-EKLDQIVAARVQRYEREHMCDSPANDSIGWRDPGFIHDAVMKKLQKGARIYY 248
           +VRYGE+ E+LDQIVAARV RYEREHMCDSPANDSIGWRDPGFIHDAVM KLQ G+R+YY
Sbjct: 198 FVRYGEKEEELDQIVAARVARYEREHMCDSPANDSIGWRDPGFIHDAVMTKLQNGSRVYY 257

Query: 249 QVGSDSKGWSSIRSFVSRNEDLDETIAFLFGDMGAATPYTTFVRTQDESISTIRWILRDI 308
           QVGS+SKGWSSI+SF+SRNED DETIAFLFGDMGAATPYTTFVRTQDESIST+RWILRDI
Sbjct: 258 QVGSESKGWSSIQSFMSRNEDSDETIAFLFGDMGAATPYTTFVRTQDESISTVRWILRDI 317

Query: 309 EALGDKPAFVSHIGDISYARGHAWLWDVFFNQIEPVASKVAYHVCIGNHEYDWPLQPWKP 368
           EALGDKPAFVSHIGDISYARGHAWLWDVFFNQIEPVAS+VAYHVCIGNHEYDWPLQPWKP
Sbjct: 318 EALGDKPAFVSHIGDISYARGHAWLWDVFFNQIEPVASRVAYHVCIGNHEYDWPLQPWKP 377

Query: 369 EWAYSIYGKDGGGECGVPYSLKFNMPGNSSEPTAAHSLPTRNLFYSFNMGSVHFVYISTE 428
           +WA  IYGKDGGGECGVPYSLKFNMPGNSSEPT  + +PTRNLFYSFNMGSVHFVYISTE
Sbjct: 378 DWASGIYGKDGGGECGVPYSLKFNMPGNSSEPTKTNGVPTRNLFYSFNMGSVHFVYISTE 437

Query: 429 TNFLQGSRQYEFIKLDLESVDRKKTPFVVVQGHRPMYTTSNELRDTPLREKMLQHLEPLF 488
           TNFL+GSRQ+ F++ DLESVDRKKTPFVVVQGHRPMYTTSNE RDTPLREKML HLEPLF
Sbjct: 438 TNFLKGSRQFSFLERDLESVDRKKTPFVVVQGHRPMYTTSNERRDTPLREKMLHHLEPLF 497

Query: 489 VKNNVTLALWGHVHRYERFCPLNNYTCGSMSVDGEEWEALPVHLVIGMAGQDWQPIWEPR 548
           VKNNVTLALWGHVHRYERFCPL NYTCGSM VDGE+WEALPVHLVIGMAGQDWQPIWEPR
Sbjct: 498 VKNNVTLALWGHVHRYERFCPLKNYTCGSMGVDGEDWEALPVHLVIGMAGQDWQPIWEPR 557

Query: 549 PNHPDDPIFPQPKRSMYRGGEFGYTRLVATKEKLTVSYVGNHDGEVHDSVEILASGQVLN 608
           PNH D PIFPQPKRSMYRGGEFGYTRLVATKEKLT+SYVGNHDGEVHDSVEILASGQV+N
Sbjct: 558 PNHLDVPIFPQPKRSMYRGGEFGYTRLVATKEKLTISYVGNHDGEVHDSVEILASGQVVN 617

Query: 609 SGIDAKFINSSLENSRTRNEMLESSFSWYVMGASILVLGAFIGYIIGFVSHARKNSISRN 668
            G+ AKF N S+ENSR+RN MLESSFSWYVMG SILVLGAFIGYI+GFVSHARK   SRN
Sbjct: 618 GGVGAKF-NESVENSRSRNGMLESSFSWYVMGGSILVLGAFIGYIVGFVSHARK---SRN 677

Query: 669 SWTPVKTEELQ 679
           +WTPVKTEELQ
Sbjct: 678 NWTPVKTEELQ 684

BLAST of Lag0003992 vs. ExPASy TrEMBL
Match: A0A6J1GI52 (Purple acid phosphatase OS=Cucurbita moschata OX=3662 GN=LOC111454362 PE=3 SV=1)

HSP 1 Score: 1223.4 bits (3164), Expect = 0.0e+00
Identity = 584/659 (88.62%), Postives = 618/659 (93.78%), Query Frame = 0

Query: 21  MNNLIPLILLISFLPFSTQSKVSISFSPSTLSKSGDPVQIQWSGIESPSNLDWLGIYSPP 80
           M NLI LILLIS  P S QSKVSIS SPSTL+KSGDPVQIQWSG+ESPS LDWLGIYSPP
Sbjct: 1   MENLIYLILLISIFPISIQSKVSISISPSTLNKSGDPVQIQWSGVESPSKLDWLGIYSPP 60

Query: 81  NSSHKHFIGYVFLSSSPGWESGFGSVSIPLVNLRSNYSFRIFRWTESEIDEKHQDHDHNP 140
           NSSHKHFIGYVFLSSSPGWESG GSV+IPLVNLRS YSFRIFRWTESEID+KH DHD+NP
Sbjct: 61  NSSHKHFIGYVFLSSSPGWESGSGSVTIPLVNLRSKYSFRIFRWTESEIDDKHHDHDNNP 120

Query: 141 LPGTAHLLAASEELQFSPGGGPEQIHLAFTDHDDEMRVMFVTEDGSERYVRYGER-EKLD 200
           LPGT HLLA+SEEL+FSPG GPEQIHLAFTD DDEMRVMFVTEDGSER VRYGE+ E+LD
Sbjct: 121 LPGTKHLLASSEELRFSPGRGPEQIHLAFTDQDDEMRVMFVTEDGSERLVRYGEKEEELD 180

Query: 201 QIVAARVQRYEREHMCDSPANDSIGWRDPGFIHDAVMKKLQKGARIYYQVGSDSKGWSSI 260
           QIVAARV RYER+HMCDSPANDSIGWRDPGFIHDAVM  LQ G+R+YYQVGS+SKGWSSI
Sbjct: 181 QIVAARVARYERKHMCDSPANDSIGWRDPGFIHDAVMTNLQNGSRVYYQVGSESKGWSSI 240

Query: 261 RSFVSRNEDLDETIAFLFGDMGAATPYTTFVRTQDESISTIRWILRDIEALGDKPAFVSH 320
           ++F+SRNED DETIAFLFGDMGAATPYTTFVRTQDESIST+RWILRDIEALGDKPAFVSH
Sbjct: 241 QNFMSRNEDSDETIAFLFGDMGAATPYTTFVRTQDESISTVRWILRDIEALGDKPAFVSH 300

Query: 321 IGDISYARGHAWLWDVFFNQIEPVASKVAYHVCIGNHEYDWPLQPWKPEWAYSIYGKDGG 380
           IGDISYARGH+WLWDVFFNQIEPVASKVAYHVCIGNHEYDWPLQPWKP+WA  IYGKDGG
Sbjct: 301 IGDISYARGHSWLWDVFFNQIEPVASKVAYHVCIGNHEYDWPLQPWKPDWASGIYGKDGG 360

Query: 381 GECGVPYSLKFNMPGNSSEPTAAHSLPTRNLFYSFNMGSVHFVYISTETNFLQGSRQYEF 440
           GECGVPYSLKFNMPGNSSEPT A+ +PTRNLFYSFNMGSVHFVYISTETNFL+GSRQ+ F
Sbjct: 361 GECGVPYSLKFNMPGNSSEPTKANGVPTRNLFYSFNMGSVHFVYISTETNFLKGSRQFSF 420

Query: 441 IKLDLESVDRKKTPFVVVQGHRPMYTTSNELRDTPLREKMLQHLEPLFVKNNVTLALWGH 500
           ++ DLESVDRKKTPFVVVQGHRPMYTTSNELRDTPLREKML HLEPLFVKNNVTLALWGH
Sbjct: 421 LERDLESVDRKKTPFVVVQGHRPMYTTSNELRDTPLREKMLHHLEPLFVKNNVTLALWGH 480

Query: 501 VHRYERFCPLNNYTCGSMSVDGEEWEALPVHLVIGMAGQDWQPIWEPRPNHPDDPIFPQP 560
           VHRYERFCPL NYTCGSM VDGE+WEALPVHLVIGMAGQDWQPIWEPRPNH D PIFPQP
Sbjct: 481 VHRYERFCPLKNYTCGSMGVDGEDWEALPVHLVIGMAGQDWQPIWEPRPNHLDVPIFPQP 540

Query: 561 KRSMYRGGEFGYTRLVATKEKLTVSYVGNHDGEVHDSVEILASGQVLNSGIDAKFINSSL 620
           KRSMYRGGEFGYTRLVATKEKLT+SYVGNHDGEVHDSVEILASGQV+N G+ AKF N S+
Sbjct: 541 KRSMYRGGEFGYTRLVATKEKLTISYVGNHDGEVHDSVEILASGQVVNGGVGAKF-NESV 600

Query: 621 ENSRTRNEMLESSFSWYVMGASILVLGAFIGYIIGFVSHARKNSISRNSWTPVKTEELQ 679
           +NSR+RN MLESSFSWYVMG SILVLGAFIGYI+GFVSHARK   SRN+WTPVKTEELQ
Sbjct: 601 KNSRSRNGMLESSFSWYVMGGSILVLGAFIGYIVGFVSHARK---SRNNWTPVKTEELQ 655

BLAST of Lag0003992 vs. ExPASy TrEMBL
Match: A0A6J1D9S2 (Purple acid phosphatase OS=Momordica charantia OX=3673 GN=LOC111018532 PE=3 SV=1)

HSP 1 Score: 1177.9 bits (3046), Expect = 0.0e+00
Identity = 563/660 (85.30%), Postives = 600/660 (90.91%), Query Frame = 0

Query: 21  MNNLIPLILL-ISFLPFSTQSKVSISFSPSTLSKSGDPVQIQWSGIESPSNLDWLGIYSP 80
           M NL  L+LL ISFLPFST SKV IS SPSTLS+SGDPV I WSGIESPS LDWLGIYSP
Sbjct: 1   MKNLFHLVLLIISFLPFSTPSKVEISLSPSTLSRSGDPVLIHWSGIESPSKLDWLGIYSP 60

Query: 81  PNSSHKHFIGYVFLSSSPGWESGFGSVSIPLVNLRSNYSFRIFRWTESEIDEKHQDHDHN 140
            NSSHKHFIGYVFLSSSPGWESG GS  +PLV+LRS+YSFRIFRWTESEID KHQDHD N
Sbjct: 61  ANSSHKHFIGYVFLSSSPGWESGAGSTVLPLVDLRSSYSFRIFRWTESEIDHKHQDHDQN 120

Query: 141 PLPGTAHLLAASEELQFSPGGGPEQIHLAFTDHDDEMRVMFVTEDGSERYVRYGE-REKL 200
           PLPGTAHLLA SEEL+FSPG GP+QIHLAFTD+DDEMRVMFVTEDGSER+VRYGE +E+L
Sbjct: 121 PLPGTAHLLATSEELRFSPGRGPDQIHLAFTDNDDEMRVMFVTEDGSERFVRYGETKEQL 180

Query: 201 DQIVAARVQRYEREHMCDSPANDSIGWRDPGFIHDAVMKKLQKGARIYYQVGSDSKGWSS 260
           DQ+ AARV RYEREH+CDSPANDSIGWRDPGFIHDAVMK L KGAR+YYQVGSDSKGWS 
Sbjct: 181 DQVAAARVGRYEREHLCDSPANDSIGWRDPGFIHDAVMKNLPKGARVYYQVGSDSKGWSD 240

Query: 261 IRSFVSRNEDLDETIAFLFGDMGAATPYTTFVRTQDESISTIRWILRDIEALGDKPAFVS 320
            +SFVSRNED DET+AFLFGDMGAATPYTTFVRTQDESIST+RWILRDIEALGDKPAFVS
Sbjct: 241 TKSFVSRNEDSDETLAFLFGDMGAATPYTTFVRTQDESISTVRWILRDIEALGDKPAFVS 300

Query: 321 HIGDISYARGHAWLWDVFFNQIEPVASKVAYHVCIGNHEYDWPLQPWKPEWAYSIYGKDG 380
           HIGDISYARGHAWLWDVFFNQIEPVASKVAYHVCIGNHEYDWPLQPWKP+WA  IYGKDG
Sbjct: 301 HIGDISYARGHAWLWDVFFNQIEPVASKVAYHVCIGNHEYDWPLQPWKPDWASGIYGKDG 360

Query: 381 GGECGVPYSLKFNMPGNSSEPTAAHSLPTRNLFYSFNMGSVHFVYISTETNFLQGSRQYE 440
           GGECGVPYSL+FNMPGNSSE T  +SLPTRNLFYSFNMGSVHFVYISTETNFL+GS+QYE
Sbjct: 361 GGECGVPYSLRFNMPGNSSERTGTNSLPTRNLFYSFNMGSVHFVYISTETNFLKGSKQYE 420

Query: 441 FIKLDLESVDRKKTPFVVVQGHRPMYTTSNELRDTPLREKMLQHLEPLFVKNNVTLALWG 500
           F+K DLESVDRKKTPFV+VQGHRPMYTTSNELRD PLREK+LQHLEPL V+N VT+ALWG
Sbjct: 421 FLKRDLESVDRKKTPFVIVQGHRPMYTTSNELRDAPLREKLLQHLEPLLVENKVTMALWG 480

Query: 501 HVHRYERFCPLNNYTCGSMSVDGEEWEALPVHLVIGMAGQDWQPIWEPRPNHPDDPIFPQ 560
           HVHRYERFCPL NYTCGSM   GE +EA PVHLVIGMAGQDWQPIWEPR NHPD P+FPQ
Sbjct: 481 HVHRYERFCPLKNYTCGSM---GESFEAFPVHLVIGMAGQDWQPIWEPRLNHPDVPVFPQ 540

Query: 561 PKRSMYRGGEFGYTRLVATKEKLTVSYVGNHDGEVHDSVEILASGQVLNSGIDAKFINSS 620
           P RSMYRGGEFGYTRLVATKEKLT+SYVGNHDGE+HDSVEILASGQVLN           
Sbjct: 541 PTRSMYRGGEFGYTRLVATKEKLTLSYVGNHDGEIHDSVEILASGQVLN----------G 600

Query: 621 LENSRTRNEMLESSFSWYVMGASILVLGAFIGYIIGFVSHARKNSISRNSWTPVKTEELQ 679
           +EN+R RNE +ESS SWYVMG SILVLG FIGYI+GFVSHARKNS+SRNSW+PVKTEELQ
Sbjct: 601 IENARARNETVESSVSWYVMGGSILVLGVFIGYIVGFVSHARKNSMSRNSWSPVKTEELQ 647

BLAST of Lag0003992 vs. TAIR 10
Match: AT1G13900.1 (Purple acid phosphatases superfamily protein )

HSP 1 Score: 957.2 bits (2473), Expect = 7.0e-279
Identity = 451/654 (68.96%), Postives = 534/654 (81.65%), Query Frame = 0

Query: 27  LILLISFLPFSTQSKVSISFSPSTLSKSGDPVQIQWSGIESPSNLDWLGIYSPPNSSHKH 86
           L+L +S    S  SK +IS SP+ L++SGD V IQWSG++SPS+LDWLG+YSPP S + H
Sbjct: 9   LLLFVSVFVSSADSKATISISPNALNRSGDSVVIQWSGVDSPSDLDWLGLYSPPESPNDH 68

Query: 87  FIGYVFLSSSPGWESGFGSVSIPLVNLRSNYSFRIFRWTESEIDEKHQDHDHNPLPGTAH 146
           FIGY FL+ S  W+ GFGS+S+PL NLRSNY+FRIFRW+ESEID KH+DHD NPLPGT H
Sbjct: 69  FIGYKFLNESSTWKDGFGSISLPLTNLRSNYTFRIFRWSESEIDPKHKDHDQNPLPGTKH 128

Query: 147 LLAASEELQFSPG-GGPEQIHLAFTDHDDEMRVMFVTEDGSERYVRYGE-REKLDQIVAA 206
           LLA SE+L F  G G PEQIHL+FT+  + MRVMFV  DG ER+VRYGE ++ L    AA
Sbjct: 129 LLAESEQLTFGSGVGMPEQIHLSFTNMVNTMRVMFVAGDGEERFVRYGESKDLLGNSAAA 188

Query: 207 RVQRYEREHMCDSPANDSIGWRDPGFIHDAVMKKLQKGARIYYQVGSDSKGWSSIRSFVS 266
           R  RYEREHMCDSPAN +IGWRDPG+I D VMK L  G R YYQVGSDSKGWS I S+++
Sbjct: 189 RGMRYEREHMCDSPANSTIGWRDPGWIFDTVMKNLNDGVRYYYQVGSDSKGWSEIHSYIA 248

Query: 267 RNEDLDETIAFLFGDMGAATPYTTFVRTQDESISTIRWILRDIEALGDKPAFVSHIGDIS 326
           R+   +ET+AF+FGDMG ATPYTTF+RTQDESIST++WILRDIEALGDKPA +SHIGDIS
Sbjct: 249 RDVTAEETVAFMFGDMGCATPYTTFIRTQDESISTVKWILRDIEALGDKPAMISHIGDIS 308

Query: 327 YARGHAWLWDVFFNQIEPVASKVAYHVCIGNHEYDWPLQPWKPEWAYSIYGKDGGGECGV 386
           YARG++W+WD FF Q+EP+AS V YHVCIGNHEYD+  QPWKP+WA SIYG DGGGECGV
Sbjct: 309 YARGYSWVWDEFFAQVEPIASTVPYHVCIGNHEYDFSTQPWKPDWAASIYGNDGGGECGV 368

Query: 387 PYSLKFNMPGNSSEPTAAHSLPTRNLFYSFNMGSVHFVYISTETNFLQGSRQYEFIKLDL 446
           PYSLKFNMPGNSSE T   + PTRNL+YS++MG+VHFVYISTETNFL+G  QYEFIK DL
Sbjct: 369 PYSLKFNMPGNSSESTGMKAPPTRNLYYSYDMGTVHFVYISTETNFLKGGSQYEFIKRDL 428

Query: 447 ESVDRKKTPFVVVQGHRPMYTTSNELRDTPLREKMLQHLEPLFVKNNVTLALWGHVHRYE 506
           ESVDRKKTPFVVVQGHRPMYTTSNE+RDT +R+KM++HLEPLFVKNNVTLALWGHVHRYE
Sbjct: 429 ESVDRKKTPFVVVQGHRPMYTTSNEVRDTMIRQKMVEHLEPLFVKNNVTLALWGHVHRYE 488

Query: 507 RFCPLNNYTCGSMSVDGEEWEALPVHLVIGMAGQDWQPIWEPRPNHPDDPIFPQPKRSMY 566
           RFCP++N TCG+      +W+  PVHLVIGMAGQDWQPIW+PRPNHPD PIFPQP++SMY
Sbjct: 489 RFCPISNNTCGT------QWQGNPVHLVIGMAGQDWQPIWQPRPNHPDLPIFPQPEQSMY 548

Query: 567 RGGEFGYTRLVATKEKLTVSYVGNHDGEVHDSVEILASGQVLNSGIDAKFINS--SLENS 626
           R GEFGYTRLVA KEKLTVS+VGNHDGEVHD+VE+LASG V++   ++  I +  ++  S
Sbjct: 549 RTGEFGYTRLVANKEKLTVSFVGNHDGEVHDTVEMLASGVVISGSKESTKIPNLKTVPAS 608

Query: 627 RTRNEMLESSFSWYVMGASILVLGAFIGYIIGFVSHARKNSISRNSWTPVKTEE 677
            T     ES+  WY  GA ++V+G  +G+IIGF +  +K+S S N W PVK EE
Sbjct: 609 ATLMGKSESNALWYAKGAGLMVVGVLLGFIIGFFTRGKKSS-SGNRWIPVKNEE 655

BLAST of Lag0003992 vs. TAIR 10
Match: AT2G03450.1 (purple acid phosphatase 9 )

HSP 1 Score: 909.1 bits (2348), Expect = 2.2e-264
Identity = 438/657 (66.67%), Postives = 517/657 (78.69%), Query Frame = 0

Query: 29  LLISFLPFSTQSKVSISFSPSTLSKSGDPVQIQWSGIESPSNLDWLGIYSPPNSSHKHFI 88
           LLIS    S  SK +IS SP TL++SGD V I+WSG+ESPS+LDWLGIYSPP+S H HFI
Sbjct: 13  LLIS----SVYSKATISISPQTLNRSGDIVVIKWSGVESPSDLDWLGIYSPPDSPHDHFI 72

Query: 89  GYVFLSSSPGWESGFGSVSIPLVNLRSNYSFRIFRWTESEIDEKHQDHDHNPLPGTAHLL 148
           GY FLS SP W+SG GS+S+PL NLRSNY+FRIF WT+SEI+ KHQDHDHNPLPGT HLL
Sbjct: 73  GYKFLSDSPTWQSGSGSISLPLTNLRSNYTFRIFHWTQSEINPKHQDHDHNPLPGTRHLL 132

Query: 149 AASEELQFS-PGGGPEQIHLAFTDHDDEMRVMFVTEDGSERYVRYGE-REKLDQIVAARV 208
             S +L F      PEQIHL++TD+ +EMRV+FVT DG ER  RYGE ++KLD I  AR 
Sbjct: 133 TESNQLNFRFAVNRPEQIHLSYTDNINEMRVVFVTGDGEEREARYGEVKDKLDNIAVARG 192

Query: 209 QRYEREHMCDSPANDSIGWRDPGFIHDAVMKKLQKGARIYYQVGSDSKGWSSIRSFVSRN 268
            RYE EHMC +PAN ++GWRDPG+  DAVMK L++G R YYQVGSD KGWS I SFVSRN
Sbjct: 193 VRYEIEHMCHAPANSTVGWRDPGWTFDAVMKNLKQGIRYYYQVGSDLKGWSEIHSFVSRN 252

Query: 269 EDLDETIAFLFGDMGAATPYTTFVRTQDESISTIRWILRDIEALG-DKPAFVSHIGDISY 328
           E  +ET+AF+FGDMG  TPYTTF+R ++ES+ST++WILRDIEALG DKP  VSHIGDISY
Sbjct: 253 EGSEETLAFMFGDMGCYTPYTTFIRGEEESLSTVKWILRDIEALGDDKPVIVSHIGDISY 312

Query: 329 ARGHAWLWDVFFNQIEPVASKVAYHVCIGNHEYDWPLQPWKPEWAYSIYGKDGGGECGVP 388
           ARG++W+WD FF QIEP+ASKV YHVCIGNHEYDWP QPWKP+WA  +YGKD GGECGVP
Sbjct: 313 ARGYSWIWDEFFTQIEPIASKVPYHVCIGNHEYDWPNQPWKPDWAAYVYGKDSGGECGVP 372

Query: 389 YSLKFNMPGNSSEPTAAHSLP-TRNLFYSFNMGSVHFVYISTETNFLQGSRQYEFIKLDL 448
           YS+KFNMPGNS+E T     P +RNL+YS++MGSVHFVYISTET+FL+G +QY F+K DL
Sbjct: 373 YSVKFNMPGNSTEATGMVKGPQSRNLYYSYDMGSVHFVYISTETDFLKGGKQYSFLKSDL 432

Query: 449 ESVDRKKTPFVVVQGHRPMYTTSNELRDTPLREKMLQHLEPLFVKNNVTLALWGHVHRYE 508
           ESV+R KTPFVVVQGHRPMYTTS ++RD  +REKM++HLEPL VKNNVT+ALWGHVHRYE
Sbjct: 433 ESVNRSKTPFVVVQGHRPMYTTSRKIRDAAIREKMIEHLEPLLVKNNVTVALWGHVHRYE 492

Query: 509 RFCPLNNYTCGSMSVDGEEWEALPVHLVIGMAGQDWQPIWEPRPNHPDDPIFPQPKRSMY 568
           RFC ++N TC      GE W+  PVHLVIGMAG+D QP+WEPR NH D PIFPQP  SMY
Sbjct: 493 RFCAISNNTC------GERWQGNPVHLVIGMAGKDSQPMWEPRANHEDVPIFPQPANSMY 552

Query: 569 RGGEFGYTRLVATKEKLTVSYVGNHDGEVHDSVEILASGQVLNSGIDAKFINSSLENSRT 628
           RGGEFGY RLVA KE+LT+SYVGNHDGEVHD VEILASG+V         I+ S + ++ 
Sbjct: 553 RGGEFGYIRLVANKERLTLSYVGNHDGEVHDVVEILASGEV---------ISGSDDGTKD 612

Query: 629 RNEMLESSFS--WYVMGASILVLGAFIGYIIGFVSHARKNS---ISRNSWTPVKTEE 677
            N   ES F+  WY+ GAS++V+G   GY +GF+S  +K S    S  SW  VK EE
Sbjct: 613 SNFGSESDFAVLWYIEGASVMVVGVIFGYFVGFLSRKKKESGVGSSNRSWIQVKNEE 650

BLAST of Lag0003992 vs. TAIR 10
Match: AT1G13750.1 (Purple acid phosphatases superfamily protein )

HSP 1 Score: 229.6 bits (584), Expect = 7.7e-60
Identity = 181/607 (29.82%), Postives = 277/607 (45.63%), Query Frame = 0

Query: 34  LPFSTQSKVSISFSPSTLSKSG---DPVQIQWSGIESPSNLDWLGIYSPPNSSHKHFIG- 93
           + F    K  +  SP+ L  +G   + V +Q+S    PS+ DW+G++SP + +     G 
Sbjct: 38  ITFGLNEKAYVKASPTVLGSNGQHSELVLVQYSS-PKPSDDDWIGVFSPADFNASTCPGD 97

Query: 94  -----------------YVFLSSSPGWESGFGSVSIPLVNLRSNYSFRIFRWTESEIDEK 153
                            Y   S+     +G GS+ + L+N RS++SF +F  +   ++ K
Sbjct: 98  NKMVQPPRLCSAPVKFQYANFSNPRYTNTGTGSLKLQLINQRSDFSFALF--SGGLLNPK 157

Query: 154 HQDHDHNPLPGTAHLLAASEELQFSPGGGPEQIHLAFTDHDDEMRVMFVTEDG---SERY 213
                         L+A S ++ F     P    LA     DEM V + +  G   +E  
Sbjct: 158 --------------LVAISNKVAFENPNAPVYPRLALGKEWDEMTVTWTSGYGLNLAEPV 217

Query: 214 VRYGEREKLDQIVAARVQRYEREHMCDSPANDSIGWRDPGFIHDAVMKKLQKGARIYYQV 273
           V +G +    ++  A    + R  MC +PA  ++GWRDPG+IH A +K+L   ++  Y+V
Sbjct: 218 VEWGVKGGERKLSPAGTLTFARNSMCGAPAR-TVGWRDPGYIHTAFLKELWPNSKYTYRV 277

Query: 274 GSD-SKG---WSSIRSFVSRNEDLDETI--AFLFGDMGAA-----TPYTTFVRTQDESIS 333
           G   S G   WS    F S       ++    +FGDMG A     + Y  F R    S++
Sbjct: 278 GHRLSNGALIWSKEYQFKSSPFPGQNSVQQVVIFGDMGKAEVDGSSEYNDFQRA---SLN 337

Query: 334 TIRWILRDIEALGDKPAFVSHIGDISYARGHAWLWDVFFNQIEPVASKVAYHVCIGNHEY 393
           T + +++D++    K   V HIGDI YA G+   WD F  QIEP+AS V Y +  GNHE 
Sbjct: 338 TTKQLIKDLK----KTDAVFHIGDICYANGYLSQWDQFIAQIEPIASTVPYMIASGNHER 397

Query: 394 DWPLQPWKPEWAYSIY-GKDGGGECGVPYSLKFNMPGNSSEPTAAHSLPTRNLFYSFNMG 453
            WP        + S Y G D GGECGVP    F +P  +             ++YS + G
Sbjct: 398 VWPN-------SGSFYEGLDSGGECGVPAETMFYVPAQN----------RAKVWYSSDYG 457

Query: 454 SVHFVYISTETNFLQGSRQYEFIKLDLESVDRKKTPFVVVQGHRPMYTTSNEL--RDTPL 513
              F    TE ++ +G+ QY FI+  L SVDR+K P+++   HR +  +S      +   
Sbjct: 458 MFRFCVADTEHDWREGTEQYNFIEHCLASVDRQKQPWLIFLAHRVLGYSSTYFYAEEGSF 517

Query: 514 REKM-LQHLEPLFVKNNVTLALWGHVHRYERFCPLNNYTCGSMSVDGEEWEAL-PVHLVI 573
            E M  + L+ L+ K  V +A++GH H YER CP+    C S      +      +H+V 
Sbjct: 518 AEPMGRESLQKLWQKYKVDIAIYGHAHNYERTCPVYQSVCTSHEKSNYKAPLNGTIHIVA 577

Query: 574 GMAGQDWQPIWEPRPNHPDDPIFPQPKRSMYRGGEFGYTRLVA-TKEKLTVSYVGNHDGE 600
           G  G       + +PN            S++R  ++G+ +L A     L   Y  + DG 
Sbjct: 578 GGGGAGLAEFSDLQPNW-----------SLFRDYDYGFLKLTAIDHSNLLFEYKKSSDGR 591

BLAST of Lag0003992 vs. TAIR 10
Match: AT5G50400.1 (purple acid phosphatase 27 )

HSP 1 Score: 222.2 bits (565), Expect = 1.2e-57
Identity = 175/599 (29.22%), Postives = 273/599 (45.58%), Query Frame = 0

Query: 44  ISFSPSTLSKSGDPVQIQWSGI----ESPSNLDWLGIYSPPN--------SSHKHF---- 103
           I  SP  L   G     +W  +      PS+ DW+G++SP          +  K      
Sbjct: 47  IHVSPLVLGSQGQ--DTEWVNVVISNPEPSSDDWVGVFSPAKFDSSSCAPTDDKEIAPFI 106

Query: 104 ----IGYVFLSSSPGW-ESGFGSVSIPLVNLRSNYSFRIFRWTESEIDEKHQDHDHNPLP 163
               + Y++  SSP + ++G   +   L+N R+++SF +F    S           NP  
Sbjct: 107 CSAPVKYMYAKSSPDYMKTGNAVLKFMLINQRADFSFALFTGGLS-----------NPT- 166

Query: 164 GTAHLLAASEELQFSPGGGPEQIHLAFTDHDDEMRVMFVTE---DGSERYVRYGEREKLD 223
               L++ S  + F     P    LA     DEM V + +      +  +V +  +    
Sbjct: 167 ----LVSVSNHVSFINPKAPVYPRLALGKKWDEMTVTWTSGYNIGEAVPFVEWSRKGTRS 226

Query: 224 QIVAARVQRYEREHMCDSPANDSIGWRDPGFIHDAVMKKLQKGARIYYQVGSD----SKG 283
           +   A    + R  MC +PA  ++GWRDPGFIH A +K L    +  Y++G +    S  
Sbjct: 227 RRSPAGTLTFTRNSMCGAPAR-TVGWRDPGFIHTASLKDLWPNLKYTYRMGHELMNGSIV 286

Query: 284 WSSIRSFVSRNEDLDETI--AFLFGDMG-----AATPYTTFVRTQDESISTIRWILRDIE 343
           WS   +F S      +++    +FGDMG      +  Y  +   Q  S++T   +++D++
Sbjct: 287 WSKNFTFKSSPYPGQDSLQRVIIFGDMGKGERDGSNEYNDY---QPGSLNTTDQLIKDLK 346

Query: 344 ALGDKPAFVSHIGDISYARGHAWLWDVFFNQIEPVASKVAYHVCIGNHEYDWPLQPWKPE 403
            +      V HIGDI+YA G+   WD F  Q+EP+AS V Y V  GNHE DWP       
Sbjct: 347 NID----IVFHIGDITYANGYISQWDQFTAQVEPIASTVPYMVASGNHERDWP------- 406

Query: 404 WAYSIY-GKDGGGECGVPYSLKFNMPGNSSEPTAAHSLPTRNLFYSFNMGSVHFVYISTE 463
            + S Y GKD GGECGVP    F+ P  +              +YS + G   F    TE
Sbjct: 407 DSGSFYGGKDSGGECGVPAETMFDFPAENK----------AKFWYSADYGMFRFCVADTE 466

Query: 464 TNFLQGSRQYEFIKLDLESVDRKKTPFVVVQGHRPMYTTSNEL--RDTPLREKM-LQHLE 523
            ++ +GS QY+FI+  L SVDR+  P+++   HR +  ++N+   ++    E M  + L+
Sbjct: 467 HDWREGSEQYQFIERCLASVDRRAQPWLIFIAHRVLGYSTNDWYGQEGSFEEPMGRESLQ 526

Query: 524 PLFVKNNVTLALWGHVHRYERFCPLNNYTCGSMSVDGEEWEAL---PVHLVIGMAGQDWQ 583
            L+ K  V +A +GHVH YER CP+    C  M  +   +       +H+V+G AG    
Sbjct: 527 KLWQKYKVDIAFYGHVHNYERTCPIYQNQC--MDNEKSHYSGAFKGTIHVVVGGAG---- 586

Query: 584 PIWEPRPNHPDDPIFPQPKRSMYRGGEFGYTRLVA-TKEKLTVSYVGNHDGEVHDSVEI 600
                  +H       +PK S++R  ++G+ +L A     L   Y  + +G VHDS  I
Sbjct: 587 -------SHLSSFSSLKPKWSIFRDYDYGFVKLTAFDHSSLLFEYKKSSNGAVHDSFTI 589

BLAST of Lag0003992 vs. TAIR 10
Match: AT4G24890.1 (purple acid phosphatase 24 )

HSP 1 Score: 219.9 bits (559), Expect = 6.1e-57
Identity = 169/604 (27.98%), Postives = 274/604 (45.36%), Query Frame = 0

Query: 40  SKVSISFSPSTLSKSGDPVQIQWSGI----ESPSNLDWLGIYSP---------PNSSHKH 99
           S V +  SP  L   G+    +W  +      P++ DW+G++SP         P S  K 
Sbjct: 46  SSVKLHASPQVLGSQGE--DTEWVNLAISNPKPTSDDWIGVFSPAKFDSGNCWPTSGGKE 105

Query: 100 F--------IGYVFLSSSPGW-ESGFGSVSIPLVNLRSNYSFRIFRWTESEIDEKHQDHD 159
                    I Y++ +S P + +SG  ++   ++N R++ SF +F    + + E      
Sbjct: 106 KTPYICSSPIKYMYCNSHPDYMKSGNVTLKFQIINQRADVSFALF---SNGVQE------ 165

Query: 160 HNPLPGTAHLLAASEELQFSPGGGPEQIHLAFTDHDDEMRVMFVTE---DGSERYVRYGE 219
                   HLL  S  + F     P    LA   + DEM V + +    D +  ++ +  
Sbjct: 166 -------PHLLGVSNPVAFFNPKAPVYPRLALGKNWDEMTVTWTSGYNIDEAVPFIEWSA 225

Query: 220 REKLDQIVAARVQRYEREHMCDSPANDSIGWRDPGFIHDAVMKKLQKGARIYYQVGSD-- 279
           +    +   A    + R  MC +PA   +GWRDPGF H + +K+L       Y++G D  
Sbjct: 226 KGLPARRSPAGTLTFNRNSMCGNPAR-GVGWRDPGFFHTSFLKELWPNREYIYRLGHDLV 285

Query: 280 --SKGWSSIRSFVSR---NEDLDETIAFLFGDMG-----AATPYTTFVRTQDESISTIRW 339
             S  WS   +FVS     +D  + +  +FGDMG      +  Y  +   Q  S++T   
Sbjct: 286 NGSTIWSKNYTFVSSPYPGQDSKQRV-IIFGDMGKGERDGSNEYNDY---QPGSLNTTDQ 345

Query: 340 ILRDIEALGDKPAFVSHIGDISYARGHAWLWDVFFNQIEPVASKVAYHVCIGNHEYDWPL 399
           +++D++ +      V HIGD++Y+ G+   WD F  Q++P+AS V Y +  GNHE DW  
Sbjct: 346 VIKDLKDID----IVFHIGDLTYSNGYLSQWDQFTAQVQPIASTVPYMIASGNHERDW-- 405

Query: 400 QPWKPEWAYSIYGKDGGGECGVPYSLKFNMPGNSSEPTAAHSLPTRNLFYSFNMGSVHFV 459
               P+      G D GGECGVP    F  P  +              +Y  + G   F 
Sbjct: 406 ----PDTGSFYAGTDSGGECGVPAETMFYFPAEN----------RAKFWYKTDYGMFRFC 465

Query: 460 YISTETNFLQGSRQYEFIKLDLESVDRKKTPFVVVQGHRPMYTTSNEL--RDTPLREKM- 519
              +E ++ +G+ QY+FI+  L +VDRK  P+++   HR +  ++N+   ++    E M 
Sbjct: 466 VADSEHDWREGTEQYKFIENCLATVDRKTQPWLIFIAHRVLGYSTNDWYGKEGTFEEPMG 525

Query: 520 LQHLEPLFVKNNVTLALWGHVHRYERFCPLNNYTCGSMSVDGEEWEAL---PVHLVIGMA 579
            + L+ L+ K  V LA +GHVH YER CP+    C  ++ D + +       +H+V+G A
Sbjct: 526 RESLQKLWQKYKVDLAFYGHVHNYERTCPIYESQC--VNNDKDHYSGTFKGTIHVVVGGA 585

Query: 580 GQDWQPIWEPRPNHPDDPIFPQPKRSMYRGGEFGYTRLVAT-KEKLTVSYVGNHDGEVHD 600
           G    P                PK S+ R  +FG+ +L A+    L   Y  +  G+V+D
Sbjct: 586 GSHLSPFSS-----------LVPKWSLVRDYDFGFVKLTASDHSSLLFEYKKSSTGQVYD 593

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
XP_038890179.10.0e+0092.26probable inactive purple acid phosphatase 2 [Benincasa hispida][more]
XP_008465701.10.0e+0090.70PREDICTED: probable inactive purple acid phosphatase 2 [Cucumis melo][more]
XP_004143791.10.0e+0089.79probable inactive purple acid phosphatase 2 [Cucumis sativus] >KGN51195.1 hypoth... [more]
XP_022973269.10.0e+0088.82probable inactive purple acid phosphatase 2 [Cucurbita maxima][more]
KAG6585839.10.0e+0088.67putative inactive purple acid phosphatase 2, partial [Cucurbita argyrosperma sub... [more]
Match NameE-valueIdentityDescription
Q9LMG79.8e-27868.96Probable inactive purple acid phosphatase 2 OS=Arabidopsis thaliana OX=3702 GN=P... [more]
Q9ZQ813.1e-26366.67Probable inactive purple acid phosphatase 9 OS=Arabidopsis thaliana OX=3702 GN=P... [more]
Q9LMX41.1e-5829.82Probable inactive purple acid phosphatase 1 OS=Arabidopsis thaliana OX=3702 GN=P... [more]
Q5MAU81.7e-5629.22Probable inactive purple acid phosphatase 27 OS=Arabidopsis thaliana OX=3702 GN=... [more]
Q8H1R28.6e-5627.98Probable inactive purple acid phosphatase 24 OS=Arabidopsis thaliana OX=3702 GN=... [more]
Match NameE-valueIdentityDescription
A0A1S3CPV00.0e+0090.70Purple acid phosphatase OS=Cucumis melo OX=3656 GN=LOC103503324 PE=3 SV=1[more]
A0A0A0KNH80.0e+0089.79Purple acid phosphatase OS=Cucumis sativus OX=3659 GN=Csa_5G487720 PE=3 SV=1[more]
A0A6J1IE320.0e+0088.82Purple acid phosphatase OS=Cucurbita maxima OX=3661 GN=LOC111471840 PE=3 SV=1[more]
A0A6J1GI520.0e+0088.62Purple acid phosphatase OS=Cucurbita moschata OX=3662 GN=LOC111454362 PE=3 SV=1[more]
A0A6J1D9S20.0e+0085.30Purple acid phosphatase OS=Momordica charantia OX=3673 GN=LOC111018532 PE=3 SV=1[more]
Match NameE-valueIdentityDescription
AT1G13900.17.0e-27968.96Purple acid phosphatases superfamily protein [more]
AT2G03450.12.2e-26466.67purple acid phosphatase 9 [more]
AT1G13750.17.7e-6029.82Purple acid phosphatases superfamily protein [more]
AT5G50400.11.2e-5729.22purple acid phosphatase 27 [more]
AT4G24890.16.1e-5727.98purple acid phosphatase 24 [more]
InterPro
Analysis Name: InterPro Annotations of Sponge gourd (AG-4) v1
Date Performed: 2022-08-01
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
NoneNo IPR availableGENE3D2.60.40.380coord: 161..267
e-value: 1.9E-15
score: 59.0
NoneNo IPR availablePANTHERPTHR45778:SF27PURPLE ACID PHOSPHATASEcoord: 27..643
NoneNo IPR availablePANTHERPTHR45778PURPLE ACID PHOSPHATASE-RELATEDcoord: 27..643
IPR004843Calcineurin-like phosphoesterase domain, ApaH typePFAMPF00149Metallophoscoord: 277..503
e-value: 3.1E-16
score: 60.5
IPR025733Iron/zinc purple acid phosphatase-like C-terminal domainPFAMPF14008Metallophos_Ccoord: 528..596
e-value: 9.7E-16
score: 58.4
IPR015914Purple acid phosphatase, N-terminalPFAMPF16656Pur_ac_phosph_Ncoord: 162..262
e-value: 7.9E-15
score: 55.2
IPR029052Metallo-dependent phosphatase-likeGENE3D3.60.21.10coord: 271..608
e-value: 3.4E-79
score: 268.6
IPR029052Metallo-dependent phosphatase-likeSUPERFAMILY56300Metallo-dependent phosphatasescoord: 276..595
IPR041792Purple acid phosphatase, metallophosphatase domainCDDcd00839MPP_PAPscoord: 271..599
e-value: 4.77464E-102
score: 311.926
IPR008963Purple acid phosphatase-like, N-terminalSUPERFAMILY49363Purple acid phosphatase, N-terminal domaincoord: 148..264

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Lag0003992.1Lag0003992.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0016311 dephosphorylation
cellular_component GO:0016021 integral component of membrane
molecular_function GO:0003993 acid phosphatase activity
molecular_function GO:0046872 metal ion binding
molecular_function GO:0016787 hydrolase activity