Lag0003986 (gene) Sponge gourd (AG‐4) v1

Overview


Name	Lag0003986
Type	gene
Organism	Luffa acutangula (Sponge gourd (AG‐4) v1)
Description	RING-type domain-containing protein
Location	chr6: 201928 .. 202671 (+)
RNA-Seq Expression	Lag0003986
Synteny	Lag0003986

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonCDSpolypeptide

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGTGGAAGTTGGCCTCCAACGCCATTGCTGGCATTCGATCTAAGAAAAACTCAACTCTTTCTAAGCAAGTTTTCTGGGAGTGCTCGGATGACGAGGTTTGCTCGGAAGCTAGTAGAGATGAAGAATTGGAATGCCCTATATGCTGGGAATCTTTTAACATTGTGGAGAACATACCTTATGTCTTATGGTGTGGTCATTCACTGTGCAAAAACTGTGTATTAGGCCTTCAAGGGTCTGTGTTGAAAGTAGCATCTCGCCAAATCCGAATCCCGATCTTCATTTCATGTCCTTGGTGTCATCAGCTATCGTTAAGGGTGGTGTACAGGGGGAACCTGAAGTTTCCCAGCAAGAACTTTTTTCTTCTTTGGATGGTTGAGAGCTTCAATGGTAATGAGGGAAAACTTGATCACTCCTTCAATGGGAATAATCAACCAGTTTTTTCTCTACCATCTGATACCAATACTTCAGAGAGGCTTTCTGTTTCTGACAGTCGTGTTAGTAGCCATGGAATAGAGAGGCGGCGGTTGTTTTTCTATAGACATTTGGATTGCCTCGTCCATTTAATCTTCAAGTTTCTACTGGTTGCCATATTTCTTCTCATAGTTGTTTTTGTAATCCCTGGAAGTGCACTCGTCTTGCTGCTCTACCTGCTAATCACTTTACTTTTTGCATTGCCATCTTTGCTGATATTTTACCTTGCAGTCCATGCTTTGGAAAAGCTTATGAGTGATATAACTTCATGA

mRNA sequence

ATGTGGAAGTTGGCCTCCAACGCCATTGCTGGCATTCGATCTAAGAAAAACTCAACTCTTTCTAAGCAAGTTTTCTGGGAGTGCTCGGATGACGAGGTTTGCTCGGAAGCTAGTAGAGATGAAGAATTGGAATGCCCTATATGCTGGGAATCTTTTAACATTGTGGAGAACATACCTTATGTCTTATGGTGTGGTCATTCACTGTGCAAAAACTGTGTATTAGGCCTTCAAGGGTCTGTGTTGAAAGTAGCATCTCGCCAAATCCGAATCCCGATCTTCATTTCATGTCCTTGGTGTCATCAGCTATCGTTAAGGGTGGTGTACAGGGGGAACCTGAAGTTTCCCAGCAAGAACTTTTTTCTTCTTTGGATGGTTGAGAGCTTCAATGGTAATGAGGGAAAACTTGATCACTCCTTCAATGGGAATAATCAACCAGTTTTTTCTCTACCATCTGATACCAATACTTCAGAGAGGCTTTCTGTTTCTGACAGTCGTGTTAGTAGCCATGGAATAGAGAGGCGGCGGTTGTTTTTCTATAGACATTTGGATTGCCTCGTCCATTTAATCTTCAAGTTTCTACTGGTTGCCATATTTCTTCTCATAGTTGTTTTTGTAATCCCTGGAAGTGCACTCGTCTTGCTGCTCTACCTGCTAATCACTTTACTTTTTGCATTGCCATCTTTGCTGATATTTTACCTTGCAGTCCATGCTTTGGAAAAGCTTATGAGTGATATAACTTCATGA

Coding sequence (CDS)

ATGTGGAAGTTGGCCTCCAACGCCATTGCTGGCATTCGATCTAAGAAAAACTCAACTCTTTCTAAGCAAGTTTTCTGGGAGTGCTCGGATGACGAGGTTTGCTCGGAAGCTAGTAGAGATGAAGAATTGGAATGCCCTATATGCTGGGAATCTTTTAACATTGTGGAGAACATACCTTATGTCTTATGGTGTGGTCATTCACTGTGCAAAAACTGTGTATTAGGCCTTCAAGGGTCTGTGTTGAAAGTAGCATCTCGCCAAATCCGAATCCCGATCTTCATTTCATGTCCTTGGTGTCATCAGCTATCGTTAAGGGTGGTGTACAGGGGGAACCTGAAGTTTCCCAGCAAGAACTTTTTTCTTCTTTGGATGGTTGAGAGCTTCAATGGTAATGAGGGAAAACTTGATCACTCCTTCAATGGGAATAATCAACCAGTTTTTTCTCTACCATCTGATACCAATACTTCAGAGAGGCTTTCTGTTTCTGACAGTCGTGTTAGTAGCCATGGAATAGAGAGGCGGCGGTTGTTTTTCTATAGACATTTGGATTGCCTCGTCCATTTAATCTTCAAGTTTCTACTGGTTGCCATATTTCTTCTCATAGTTGTTTTTGTAATCCCTGGAAGTGCACTCGTCTTGCTGCTCTACCTGCTAATCACTTTACTTTTTGCATTGCCATCTTTGCTGATATTTTACCTTGCAGTCCATGCTTTGGAAAAGCTTATGAGTGATATAACTTCATGA

Protein sequence

MWKLASNAIAGIRSKKNSTLSKQVFWECSDDEVCSEASRDEELECPICWESFNIVENIPYVLWCGHSLCKNCVLGLQGSVLKVASRQIRIPIFISCPWCHQLSLRVVYRGNLKFPSKNFFLLWMVESFNGNEGKLDHSFNGNNQPVFSLPSDTNTSERLSVSDSRVSSHGIERRRLFFYRHLDCLVHLIFKFLLVAIFLLIVVFVIPGSALVLLLYLLITLLFALPSLLIFYLAVHALEKLMSDITS

Homology

[Close]

BLAST of Lag0003986 vs. NCBI nr
Match: KAG7029990.1 (hypothetical protein SDJN02_08334, partial [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 416.4 bits (1069), Expect = 1.7e-112
Identity = 211/249 (84.74%), Postives = 228/249 (91.57%), Query Frame = 0


Query: 1   MWKLASNAIAGIRSKKNSTLSKQVFWECSDDEVCSEASRDEELECPICWESFNIVENIPY 60
           MWKLASNA AGIRSK NS +SKQ FWECSDDEVCSE SRDEELECPICWESFNIVEN+PY
Sbjct: 1   MWKLASNAFAGIRSKNNSAVSKQAFWECSDDEVCSEDSRDEELECPICWESFNIVENVPY 60

Query: 61  VLWCGHSLCKNCVLGLQGSVLKVASRQIRIPIFISCPWCHQLSLRVVYRGNLKFPSKNFF 120
           VLWCGH+LC+NCVLGLQG VLK+A+RQIRIPIFISCPWCHQLSLRVVY+GNLKFPSKNFF
Sbjct: 61  VLWCGHTLCRNCVLGLQGYVLKLATRQIRIPIFISCPWCHQLSLRVVYKGNLKFPSKNFF 120

Query: 121 LLWMVESFNGNEGKLDHSFNGNNQPVFSL-PSDTNTSERLSVSDSRVSSHGIE-RRRLFF 180
           LLWMVES NGNEGKLDH FNG+NQPVFSL PSD N S+R S SD RVS HGIE RRRLF 
Sbjct: 121 LLWMVESLNGNEGKLDHPFNGDNQPVFSLPPSDANASQRHSFSDGRVSRHGIERRRRLFS 180

Query: 181 YRHLDCLVHLIFKFLLVAIFLLIVVFVIPGSALVLLLYLLITLLFALPSLLIFYLAVHAL 240
           Y HLDC++HLIFKFLL  IF+LIVVFVIPGSAL+LLLYLLIT LFALPSLLIFY+++HAL
Sbjct: 181 YEHLDCVIHLIFKFLLAVIFVLIVVFVIPGSALILLLYLLITFLFALPSLLIFYISLHAL 240

Query: 241 EKLMSDITS 248
           EKLM+DITS
Sbjct: 241 EKLMNDITS 249

[Close]

BLAST of Lag0003986 vs. NCBI nr
Match: KAG6599045.1 (hypothetical protein SDJN03_08823, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 414.5 bits (1064), Expect = 6.6e-112
Identity = 210/249 (84.34%), Postives = 228/249 (91.57%), Query Frame = 0


Query: 1   MWKLASNAIAGIRSKKNSTLSKQVFWECSDDEVCSEASRDEELECPICWESFNIVENIPY 60
           MWKLASNA AGIRSK NS +SKQ FWECSDDEVCSE SRDEELECPICWESFNIVEN+PY
Sbjct: 1   MWKLASNAFAGIRSKNNSAVSKQAFWECSDDEVCSEDSRDEELECPICWESFNIVENVPY 60

Query: 61  VLWCGHSLCKNCVLGLQGSVLKVASRQIRIPIFISCPWCHQLSLRVVYRGNLKFPSKNFF 120
           VLWCGH+LC+NCVLGLQG VLK+A+RQIRIPIFISCPWCHQLSLRVVY+GNLKFPSKNFF
Sbjct: 61  VLWCGHTLCRNCVLGLQGYVLKLATRQIRIPIFISCPWCHQLSLRVVYKGNLKFPSKNFF 120

Query: 121 LLWMVESFNGNEGKLDHSFNGNNQPVFSL-PSDTNTSERLSVSDSRVSSHGIE-RRRLFF 180
           LLWMVES NGNEGKLDH FNG+NQPVFSL PSD + S+R S SD RVS HGIE RRRLF 
Sbjct: 121 LLWMVESLNGNEGKLDHPFNGDNQPVFSLPPSDADASQRHSFSDGRVSRHGIERRRRLFS 180

Query: 181 YRHLDCLVHLIFKFLLVAIFLLIVVFVIPGSALVLLLYLLITLLFALPSLLIFYLAVHAL 240
           Y HLDC++HLIFKFLL  IF+LIVVFVIPGSAL+LLLYLLIT LFALPSLLIFY+++HAL
Sbjct: 181 YEHLDCVIHLIFKFLLAVIFVLIVVFVIPGSALILLLYLLITFLFALPSLLIFYISLHAL 240

Query: 241 EKLMSDITS 248
           EKLM+DITS
Sbjct: 241 EKLMNDITS 249

[Close]

BLAST of Lag0003986 vs. NCBI nr
Match: XP_023547098.1 (uncharacterized protein LOC111806010 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 414.1 bits (1063), Expect = 8.6e-112
Identity = 209/249 (83.94%), Postives = 228/249 (91.57%), Query Frame = 0


Query: 1   MWKLASNAIAGIRSKKNSTLSKQVFWECSDDEVCSEASRDEELECPICWESFNIVENIPY 60
           MWKLASNA AGI+SK NS +SKQ FWECSDDEVCSE SRDEELECPICWESFNIVEN+PY
Sbjct: 1   MWKLASNAFAGIQSKNNSAVSKQAFWECSDDEVCSEDSRDEELECPICWESFNIVENVPY 60

Query: 61  VLWCGHSLCKNCVLGLQGSVLKVASRQIRIPIFISCPWCHQLSLRVVYRGNLKFPSKNFF 120
           VLWCGH+LC+NCVLGLQG VLK+A+RQIRIPIFI CPWCHQLSLRVVY+GNLKFPSKNFF
Sbjct: 61  VLWCGHTLCRNCVLGLQGYVLKLATRQIRIPIFIPCPWCHQLSLRVVYKGNLKFPSKNFF 120

Query: 121 LLWMVESFNGNEGKLDHSFNGNNQPVFSL-PSDTNTSERLSVSDSRVSSHGIE-RRRLFF 180
           LLWMVES NGNEGKLDH FNG+NQPVFSL PSD N S+R S SD RVS HGIE RRRLF 
Sbjct: 121 LLWMVESLNGNEGKLDHPFNGDNQPVFSLPPSDANASQRHSFSDGRVSRHGIERRRRLFC 180

Query: 181 YRHLDCLVHLIFKFLLVAIFLLIVVFVIPGSALVLLLYLLITLLFALPSLLIFYLAVHAL 240
           Y HLDC++HL+FKFLLV IF+LIVVFVIPGSAL+LLLYLLIT LFALPSLLIFY+++HAL
Sbjct: 181 YEHLDCVIHLVFKFLLVVIFVLIVVFVIPGSALILLLYLLITFLFALPSLLIFYISLHAL 240

Query: 241 EKLMSDITS 248
           EKLM+DITS
Sbjct: 241 EKLMNDITS 249

[Close]

BLAST of Lag0003986 vs. NCBI nr
Match: XP_022999337.1 (uncharacterized protein LOC111493743 [Cucurbita maxima])

HSP 1 Score: 413.3 bits (1061), Expect = 1.5e-111
Identity = 210/249 (84.34%), Postives = 228/249 (91.57%), Query Frame = 0


Query: 1   MWKLASNAIAGIRSKKNSTLSKQVFWECSDDEVCSEASRDEELECPICWESFNIVENIPY 60
           MWKLASNA AGIRSK NS +SKQ FWECSDDEVCSE SRDEELECPICWESFNIVEN+PY
Sbjct: 1   MWKLASNAFAGIRSKNNSAVSKQAFWECSDDEVCSEDSRDEELECPICWESFNIVENVPY 60

Query: 61  VLWCGHSLCKNCVLGLQGSVLKVASRQIRIPIFISCPWCHQLSLRVVYRGNLKFPSKNFF 120
           VLWCGH+LC+NCVLGLQG VLK+A+RQIRIPIFISCPWCHQLSLRVVY+GNLKFPSKNFF
Sbjct: 61  VLWCGHTLCRNCVLGLQGYVLKLATRQIRIPIFISCPWCHQLSLRVVYKGNLKFPSKNFF 120

Query: 121 LLWMVESFNGNEGKLDHSFNGNNQPVFSL-PSDTNTSERLSVSDSRVSSHGIERRR-LFF 180
           LLWMVES NGNEGKLDH FNG+NQPVFSL PSD + S+R S SD RV+ HGIERR  LF 
Sbjct: 121 LLWMVESLNGNEGKLDHPFNGDNQPVFSLPPSDADASQRHSFSDGRVNRHGIERRPWLFS 180

Query: 181 YRHLDCLVHLIFKFLLVAIFLLIVVFVIPGSALVLLLYLLITLLFALPSLLIFYLAVHAL 240
           Y HLDCL+HLIFKFLLV IF+LIVVFVIPGSAL+LLLYLLIT LFALPSLLIFY+++HAL
Sbjct: 181 YEHLDCLIHLIFKFLLVVIFVLIVVFVIPGSALILLLYLLITFLFALPSLLIFYISLHAL 240

Query: 241 EKLMSDITS 248
           EKLM+DITS
Sbjct: 241 EKLMNDITS 249

[Close]

BLAST of Lag0003986 vs. NCBI nr
Match: XP_022946373.1 (uncharacterized protein LOC111450453 [Cucurbita moschata])

HSP 1 Score: 412.9 bits (1060), Expect = 1.9e-111
Identity = 209/249 (83.94%), Postives = 228/249 (91.57%), Query Frame = 0


Query: 1   MWKLASNAIAGIRSKKNSTLSKQVFWECSDDEVCSEASRDEELECPICWESFNIVENIPY 60
           MWKLASNA AGIRSK NS +SKQ FWECSDDEVCSE SRDEELECPICWESFNIVEN+PY
Sbjct: 1   MWKLASNAFAGIRSKNNSAVSKQAFWECSDDEVCSEDSRDEELECPICWESFNIVENVPY 60

Query: 61  VLWCGHSLCKNCVLGLQGSVLKVASRQIRIPIFISCPWCHQLSLRVVYRGNLKFPSKNFF 120
           VLWCGH+LC+NCVLGLQG VLK+A+RQIRIPIFISCPWCHQLSLRVVY+GNLKFPSKNFF
Sbjct: 61  VLWCGHTLCRNCVLGLQGYVLKLATRQIRIPIFISCPWCHQLSLRVVYKGNLKFPSKNFF 120

Query: 121 LLWMVESFNGNEGKLDHSFNGNNQPVFSL-PSDTNTSERLSVSDSRVSSHGIE-RRRLFF 180
           LLWMVES NGNEGKLDH FNG+NQPVFSL PSD + S+R S SD RVS HGIE RRRLF 
Sbjct: 121 LLWMVESLNGNEGKLDHPFNGDNQPVFSLPPSDADASQRHSFSDGRVSRHGIERRRRLFS 180

Query: 181 YRHLDCLVHLIFKFLLVAIFLLIVVFVIPGSALVLLLYLLITLLFALPSLLIFYLAVHAL 240
           Y HLDC++HLIFKFLL  IF+LIVVFVIPGSAL+LLLYLLIT LFALPSLLIFY+++HAL
Sbjct: 181 YEHLDCVIHLIFKFLLAVIFVLIVVFVIPGSALILLLYLLITFLFALPSLLIFYISLHAL 240

Query: 241 EKLMSDITS 248
           EKLM+DI+S
Sbjct: 241 EKLMNDISS 249

[Close]

BLAST of Lag0003986 vs. ExPASy Swiss-Prot
Match: Q9H0X6 (RING finger protein 208 OS=Homo sapiens OX=9606 GN=RNF208 PE=1 SV=2)

HSP 1 Score: 52.8 bits (125), Expect = 6.6e-06
Identity = 27/67 (40.30%), Postives = 35/67 (52.24%), Query Frame = 0


Query: 35  SEASRDEELECPICWESFNIVENIPYVLWCGHSLCKNCVLGLQGSVLKVASRQIRIPIFI 94
           S A+  E LECP C  S+N+ +  P VL C HS+C+ C+  L  S  K          FI
Sbjct: 133 SSAAAGEPLECPTCGHSYNVTQRRPRVLSCLHSVCEQCLQILYESCPKYK--------FI 191

Query: 95  SCPWCHQ 102
           SCP C +
Sbjct: 193 SCPTCRR 191

[Close]

BLAST of Lag0003986 vs. ExPASy Swiss-Prot
Match: Q8K0W3 (RING finger protein 208 OS=Mus musculus OX=10090 GN=Rnf208 PE=1 SV=2)

HSP 1 Score: 52.0 bits (123), Expect = 1.1e-05
Identity = 25/61 (40.98%), Postives = 33/61 (54.10%), Query Frame = 0


Query: 41  EELECPICWESFNIVENIPYVLWCGHSLCKNCVLGLQGSVLKVASRQIRIPIFISCPWCH 100
           E LECP C  ++N+ +  P VL C HS+C+ C+  L  S  K          FISCP CH
Sbjct: 143 EPLECPTCGHTYNVTQRRPRVLSCLHSVCEQCLQILYESCPKYK--------FISCPTCH 195

Query: 101 Q 102
           +
Sbjct: 203 R 195

[Close]

BLAST of Lag0003986 vs. ExPASy Swiss-Prot
Match: Q96D59 (E3 ubiquitin-protein ligase RNF183 OS=Homo sapiens OX=9606 GN=RNF183 PE=1 SV=2)

HSP 1 Score: 48.5 bits (114), Expect = 1.2e-04
Identity = 24/69 (34.78%), Postives = 35/69 (50.72%), Query Frame = 0


Query: 33  VCSEASRDEELECPICWESFNIVENIPYVLWCGHSLCKNCVLGLQGSVLKVASRQIRIPI 92
           +  +  R+ E ECP+CW  FN   + P +L C HS C  C+  L  S++  A R+     
Sbjct: 1   MAEQQGRELEAECPVCWNPFNNTFHTPKMLDCCHSFCVECLAHL--SLVTPARRR----- 60

Query: 93  FISCPWCHQ 102
            + CP C Q
Sbjct: 61  -LLCPLCRQ 61

[Close]

BLAST of Lag0003986 vs. ExPASy Swiss-Prot
Match: Q13049 (E3 ubiquitin-protein ligase TRIM32 OS=Homo sapiens OX=9606 GN=TRIM32 PE=1 SV=2)

HSP 1 Score: 47.8 bits (112), Expect = 2.1e-04
Identity = 22/63 (34.92%), Postives = 34/63 (53.97%), Query Frame = 0


Query: 41  EELECPICWESFNIVENIPYVLWCGHSLCKNCVLGLQGSVLKVASRQIRIPIFISCPWCH 100
           E LECPIC ESF   +  P +L CGH++C+ C+  L  S +            + CP+C 
Sbjct: 16  EVLECPICMESFTEEQLRPKLLHCGHTICRQCLEKLLASSIN----------GVRCPFCS 68

Query: 101 QLS 104
           +++
Sbjct: 76  KIT 68

[Close]

BLAST of Lag0003986 vs. ExPASy Swiss-Prot
Match: Q8CH72 (E3 ubiquitin-protein ligase TRIM32 OS=Mus musculus OX=10090 GN=Trim32 PE=1 SV=2)

HSP 1 Score: 47.8 bits (112), Expect = 2.1e-04
Identity = 22/63 (34.92%), Postives = 34/63 (53.97%), Query Frame = 0


Query: 41  EELECPICWESFNIVENIPYVLWCGHSLCKNCVLGLQGSVLKVASRQIRIPIFISCPWCH 100
           E LECPIC ESF   +  P +L CGH++C+ C+  L  S +            + CP+C 
Sbjct: 17  EVLECPICMESFTEEQLRPKLLHCGHTICRQCLEKLLASSIN----------GVRCPFCS 69

Query: 101 QLS 104
           +++
Sbjct: 77  KIT 69

[Close]

BLAST of Lag0003986 vs. ExPASy TrEMBL
Match: A0A6J1KAL8 (uncharacterized protein LOC111493743 OS=Cucurbita maxima OX=3661 GN=LOC111493743 PE=4 SV=1)

HSP 1 Score: 413.3 bits (1061), Expect = 7.1e-112
Identity = 210/249 (84.34%), Postives = 228/249 (91.57%), Query Frame = 0


Query: 1   MWKLASNAIAGIRSKKNSTLSKQVFWECSDDEVCSEASRDEELECPICWESFNIVENIPY 60
           MWKLASNA AGIRSK NS +SKQ FWECSDDEVCSE SRDEELECPICWESFNIVEN+PY
Sbjct: 1   MWKLASNAFAGIRSKNNSAVSKQAFWECSDDEVCSEDSRDEELECPICWESFNIVENVPY 60

Query: 61  VLWCGHSLCKNCVLGLQGSVLKVASRQIRIPIFISCPWCHQLSLRVVYRGNLKFPSKNFF 120
           VLWCGH+LC+NCVLGLQG VLK+A+RQIRIPIFISCPWCHQLSLRVVY+GNLKFPSKNFF
Sbjct: 61  VLWCGHTLCRNCVLGLQGYVLKLATRQIRIPIFISCPWCHQLSLRVVYKGNLKFPSKNFF 120

Query: 121 LLWMVESFNGNEGKLDHSFNGNNQPVFSL-PSDTNTSERLSVSDSRVSSHGIERRR-LFF 180
           LLWMVES NGNEGKLDH FNG+NQPVFSL PSD + S+R S SD RV+ HGIERR  LF 
Sbjct: 121 LLWMVESLNGNEGKLDHPFNGDNQPVFSLPPSDADASQRHSFSDGRVNRHGIERRPWLFS 180

Query: 181 YRHLDCLVHLIFKFLLVAIFLLIVVFVIPGSALVLLLYLLITLLFALPSLLIFYLAVHAL 240
           Y HLDCL+HLIFKFLLV IF+LIVVFVIPGSAL+LLLYLLIT LFALPSLLIFY+++HAL
Sbjct: 181 YEHLDCLIHLIFKFLLVVIFVLIVVFVIPGSALILLLYLLITFLFALPSLLIFYISLHAL 240

Query: 241 EKLMSDITS 248
           EKLM+DITS
Sbjct: 241 EKLMNDITS 249

[Close]

BLAST of Lag0003986 vs. ExPASy TrEMBL
Match: A0A6J1G3L5 (uncharacterized protein LOC111450453 OS=Cucurbita moschata OX=3662 GN=LOC111450453 PE=4 SV=1)

HSP 1 Score: 412.9 bits (1060), Expect = 9.3e-112
Identity = 209/249 (83.94%), Postives = 228/249 (91.57%), Query Frame = 0


Query: 1   MWKLASNAIAGIRSKKNSTLSKQVFWECSDDEVCSEASRDEELECPICWESFNIVENIPY 60
           MWKLASNA AGIRSK NS +SKQ FWECSDDEVCSE SRDEELECPICWESFNIVEN+PY
Sbjct: 1   MWKLASNAFAGIRSKNNSAVSKQAFWECSDDEVCSEDSRDEELECPICWESFNIVENVPY 60

Query: 61  VLWCGHSLCKNCVLGLQGSVLKVASRQIRIPIFISCPWCHQLSLRVVYRGNLKFPSKNFF 120
           VLWCGH+LC+NCVLGLQG VLK+A+RQIRIPIFISCPWCHQLSLRVVY+GNLKFPSKNFF
Sbjct: 61  VLWCGHTLCRNCVLGLQGYVLKLATRQIRIPIFISCPWCHQLSLRVVYKGNLKFPSKNFF 120

Query: 121 LLWMVESFNGNEGKLDHSFNGNNQPVFSL-PSDTNTSERLSVSDSRVSSHGIE-RRRLFF 180
           LLWMVES NGNEGKLDH FNG+NQPVFSL PSD + S+R S SD RVS HGIE RRRLF 
Sbjct: 121 LLWMVESLNGNEGKLDHPFNGDNQPVFSLPPSDADASQRHSFSDGRVSRHGIERRRRLFS 180

Query: 181 YRHLDCLVHLIFKFLLVAIFLLIVVFVIPGSALVLLLYLLITLLFALPSLLIFYLAVHAL 240
           Y HLDC++HLIFKFLL  IF+LIVVFVIPGSAL+LLLYLLIT LFALPSLLIFY+++HAL
Sbjct: 181 YEHLDCVIHLIFKFLLAVIFVLIVVFVIPGSALILLLYLLITFLFALPSLLIFYISLHAL 240

Query: 241 EKLMSDITS 248
           EKLM+DI+S
Sbjct: 241 EKLMNDISS 249

[Close]

BLAST of Lag0003986 vs. ExPASy TrEMBL
Match: A0A1S3CPH8 (uncharacterized protein LOC103503329 OS=Cucumis melo OX=3656 GN=LOC103503329 PE=4 SV=1)

HSP 1 Score: 404.8 bits (1039), Expect = 2.5e-109
Identity = 207/247 (83.81%), Postives = 222/247 (89.88%), Query Frame = 0


Query: 1   MWKLASNAIAGIRSKKNSTLSKQVFWECSDDEVCSEASRDEELECPICWESFNIVENIPY 60
           MWKLASNAIAGIRSKKNS + KQVFWECSDDEVCSEASRDEELECPICWESFNIVEN+PY
Sbjct: 1   MWKLASNAIAGIRSKKNSDVPKQVFWECSDDEVCSEASRDEELECPICWESFNIVENVPY 60

Query: 61  VLWCGHSLCKNCVLGLQGSVLKVASRQIRIPIFISCPWCHQLSLRVVYRGNLKFPSKNFF 120
           VLWCGHSLCKNCVLGLQGSVLK+A+RQ+RIPI ISCPWCHQLSLRVVY+GNLKFPSKNFF
Sbjct: 61  VLWCGHSLCKNCVLGLQGSVLKLATRQMRIPIVISCPWCHQLSLRVVYKGNLKFPSKNFF 120

Query: 121 LLWMVESFNGNEGKLDHSFNGNNQPVFSLPSDTNTSERLSVSDSRVSSHGIERRRLFFYR 180
           LLWMVESFNGNEGKLDHSFN +NQ VFSLPS T TS               ERRRLFFY 
Sbjct: 121 LLWMVESFNGNEGKLDHSFNSDNQSVFSLPSGTVTS---------------ERRRLFFYN 180

Query: 181 HLDCLVHLIFKFLLVAIFLLIVVFVIPGSALVLLLYLLITLLFALPSLLIFYLAVHALEK 240
           HLDC++HL+FKFLL  IF+LIVVFVIPGSAL+LLLYLLITLLFALPSLLIFYLA+HAL+K
Sbjct: 181 HLDCVIHLVFKFLLTVIFVLIVVFVIPGSALILLLYLLITLLFALPSLLIFYLALHALDK 232

Query: 241 LMSDITS 248
           LM+DITS
Sbjct: 241 LMNDITS 232

[Close]

BLAST of Lag0003986 vs. ExPASy TrEMBL
Match: A0A5A7TGX0 (Zf-RING_LisH domain-containing protein OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold278G00120 PE=4 SV=1)

HSP 1 Score: 404.8 bits (1039), Expect = 2.5e-109
Identity = 207/247 (83.81%), Postives = 222/247 (89.88%), Query Frame = 0


Query: 1   MWKLASNAIAGIRSKKNSTLSKQVFWECSDDEVCSEASRDEELECPICWESFNIVENIPY 60
           MWKLASNAIAGIRSKKNS + KQVFWECSDDEVCSEASRDEELECPICWESFNIVEN+PY
Sbjct: 1   MWKLASNAIAGIRSKKNSDVPKQVFWECSDDEVCSEASRDEELECPICWESFNIVENVPY 60

Query: 61  VLWCGHSLCKNCVLGLQGSVLKVASRQIRIPIFISCPWCHQLSLRVVYRGNLKFPSKNFF 120
           VLWCGHSLCKNCVLGLQGSVLK+A+RQ+RIPI ISCPWCHQLSLRVVY+GNLKFPSKNFF
Sbjct: 61  VLWCGHSLCKNCVLGLQGSVLKLATRQMRIPIVISCPWCHQLSLRVVYKGNLKFPSKNFF 120

Query: 121 LLWMVESFNGNEGKLDHSFNGNNQPVFSLPSDTNTSERLSVSDSRVSSHGIERRRLFFYR 180
           LLWMVESFNGNEGKLDHSFN +NQ VFSLPS T TS               ERRRLFFY 
Sbjct: 121 LLWMVESFNGNEGKLDHSFNSDNQSVFSLPSGTVTS---------------ERRRLFFYN 180

Query: 181 HLDCLVHLIFKFLLVAIFLLIVVFVIPGSALVLLLYLLITLLFALPSLLIFYLAVHALEK 240
           HLDC++HL+FKFLL  IF+LIVVFVIPGSAL+LLLYLLITLLFALPSLLIFYLA+HAL+K
Sbjct: 181 HLDCVIHLVFKFLLTVIFVLIVVFVIPGSALILLLYLLITLLFALPSLLIFYLALHALDK 232

Query: 241 LMSDITS 248
           LM+DITS
Sbjct: 241 LMNDITS 232

[Close]

BLAST of Lag0003986 vs. ExPASy TrEMBL
Match: A0A6J1D940 (uncharacterized protein LOC111018502 OS=Momordica charantia OX=3673 GN=LOC111018502 PE=4 SV=1)

HSP 1 Score: 404.8 bits (1039), Expect = 2.5e-109
Identity = 212/251 (84.46%), Postives = 229/251 (91.24%), Query Frame = 0


Query: 1   MWKLASNAIAGIRSKKNSTLSKQVFWECSDDEVCSEASRDEELECPICWESFNIVENIPY 60
           MWKLASNAIAGIR +KNS +SKQVFWECSDDEVCSEASRDE LECPICWESFNIVENIPY
Sbjct: 1   MWKLASNAIAGIRLRKNSVVSKQVFWECSDDEVCSEASRDEGLECPICWESFNIVENIPY 60

Query: 61  VLWCGHSLCKNCVLGLQGSVLKVASRQIRIPIFISCPWCHQLSLRVVYRGNLKFPSKNFF 120
           VLWCGH+LCKNCVLGLQGSVLKVA+RQIR+PI ISCPWCHQLSLR+VY+GNLKFPSKNFF
Sbjct: 61  VLWCGHTLCKNCVLGLQGSVLKVATRQIRVPICISCPWCHQLSLRLVYKGNLKFPSKNFF 120

Query: 121 LLWMVESFNGNEGKLDHSFNGNNQPVFSLPSDTNT--SERLSVSDSRVSSHGIERRR--L 180
           LLWMVESFN +EGKLDHSFNG+NQPVF LPSDT T  SERL   DS VS HGI RRR  L
Sbjct: 121 LLWMVESFNDDEGKLDHSFNGDNQPVFPLPSDTETVSSERL---DSHVSRHGIGRRRRWL 180

Query: 181 FFYRHLDCLVHLIFKFLLVAIFLLIVVFVIPGSALVLLLYLLITLLFALPSLLIFYLAVH 240
           F Y++LD L+HLI KFLLV IFLLIVVFVIPGSA++LLLYLLIT LFALPSLL+FYLA+H
Sbjct: 181 FVYKYLDWLIHLISKFLLVVIFLLIVVFVIPGSAVILLLYLLITFLFALPSLLVFYLALH 240

Query: 241 ALEKLMSDITS 248
           ALE+LMSDITS
Sbjct: 241 ALERLMSDITS 248

[Close]

BLAST of Lag0003986 vs. TAIR 10
Match: AT1G69330.1 (RING/U-box superfamily protein )

HSP 1 Score: 228.0 bits (580), Expect = 8.2e-60
Identity = 140/275 (50.91%), Postives = 176/275 (64.00%), Query Frame = 0


Query: 1   MWKLASNAIAGIRSKKNSTLSKQVFWECSDDEVCSEASRDEE-LECPICWESFNIVENIP 60
           MW  ASN I  +  KK+   S Q   +CSDDEV S  SRDEE LECPICWESFNIVEN+P
Sbjct: 1   MWGFASNVIGSMGLKKSPKDSAQASSQCSDDEV-SNISRDEEGLECPICWESFNIVENVP 60

Query: 61  YVLWCGHSLCKNCVLGLQGSVLKVASRQIRIPIFISCPWCHQLSLRVVYRGNLKFPSKNF 120
           YVLWCGH+LC+NCV GLQ +VL+++S+ IRIP F+SCPWC  LS R+VY+GNLKFP KNF
Sbjct: 61  YVLWCGHTLCQNCVFGLQSAVLRLSSQDIRIPFFVSCPWCQLLSFRIVYKGNLKFPRKNF 120

Query: 121 FLLWMVESFNGNEGKLDHSFNGNNQPVFSLPSDTNTS---ERLSVSDSRVSSHG--IER- 180
           FLLWMVES NG     D + +G+     SL +D   S    R S+S    SS+   + R 
Sbjct: 121 FLLWMVESLNG-----DRTSHGDRTSHGSLVTDNQQSAPTPRCSMSLGNHSSNNNLVARP 180

Query: 181 --------------------RRLF-FYRHLDCLVHLIFKFLLVAIFLLIVVFVIPGSALV 240
                               R+LF F++ LD  +    KF  V IFLLIV F IPGS ++
Sbjct: 181 LLRNQSTDLLPHHDHSNQPSRQLFSFHKSLDFFISFTSKFPFVIIFLLIVFFAIPGSLII 240

Query: 241 LLLYLLITLLFALPSLLIFYLAVHALEKLMSDITS 248
           L LY L+T+LFA+P+ L+ Y A   LE+L+++ITS
Sbjct: 241 LALYFLLTILFAVPAGLVLYFAYPILERLVNEITS 269

[Close]

BLAST of Lag0003986 vs. TAIR 10
Match: AT3G29270.1 (RING/U-box superfamily protein )

HSP 1 Score: 212.6 bits (540), Expect = 3.5e-55
Identity = 120/261 (45.98%), Postives = 166/261 (63.60%), Query Frame = 0


Query: 1   MWKLASNAIAG-IRSKKNSTLSKQVFWECSDDEVCSEASRDEELECPICWESFNIVENIP 60
           MW LAS+ + G I  K +         ECSDD+V S  S+DE LECPICWESFNIVEN+P
Sbjct: 9   MWNLASSYLTGNIGPKNDIRRPVHAHAECSDDDVSSVGSKDEGLECPICWESFNIVENVP 68

Query: 61  YVLWCGHSLCKNCVLGLQGSVLKVASRQIRIPIFISCPWCHQLSLRVVYRGNLKFPSKNF 120
           YVLWCGH++CKNC+LGLQ +++K+ +  +++P+FISCPWC+ LS R+V+RG L+FP KN+
Sbjct: 69  YVLWCGHTMCKNCILGLQWAIVKLPTHPVQLPLFISCPWCNLLSFRLVFRGTLRFPHKNY 128

Query: 121 FLLWMVESFNG----------NEGKLDHSFNGNNQPVFSLPSDTNTSERLSVSDSRVSSH 180
           F+LWMVE  NG           +G  DH+    +  + +    +      SV+D R+   
Sbjct: 129 FVLWMVERMNGERRNSPGGAQTDGNNDHTRETPSPCLHNRHHRSQPEPSRSVNDHRIPRD 188

Query: 181 GIE---RRRLFFYRHLDCLVHLIFKFLLVAIFLLIVVFVIPGSALVLLLYLLITLLFALP 240
            I+   R+ L F+      V L  KF LV IFLLI+++ IP SA +L +Y+L+TLL ALP
Sbjct: 189 NIQTSLRKSLVFF------VQLTAKFPLVVIFLLIILYAIPTSAAILAMYILVTLLLALP 248

Query: 241 SLLIFYLAVHALEKLMSDITS 248
           S LI Y A   L+ L+ +I +
Sbjct: 249 SFLILYFAYPCLDWLVREIVT 263

[Close]

BLAST of Lag0003986 vs. TAIR 10
Match: AT3G29270.2 (RING/U-box superfamily protein )

HSP 1 Score: 212.6 bits (540), Expect = 3.5e-55
Identity = 120/261 (45.98%), Postives = 166/261 (63.60%), Query Frame = 0


Query: 1   MWKLASNAIAG-IRSKKNSTLSKQVFWECSDDEVCSEASRDEELECPICWESFNIVENIP 60
           MW LAS+ + G I  K +         ECSDD+V S  S+DE LECPICWESFNIVEN+P
Sbjct: 9   MWNLASSYLTGNIGPKNDIRRPVHAHAECSDDDVSSVGSKDEGLECPICWESFNIVENVP 68

Query: 61  YVLWCGHSLCKNCVLGLQGSVLKVASRQIRIPIFISCPWCHQLSLRVVYRGNLKFPSKNF 120
           YVLWCGH++CKNC+LGLQ +++K+ +  +++P+FISCPWC+ LS R+V+RG L+FP KN+
Sbjct: 69  YVLWCGHTMCKNCILGLQWAIVKLPTHPVQLPLFISCPWCNLLSFRLVFRGTLRFPHKNY 128

Query: 121 FLLWMVESFNG----------NEGKLDHSFNGNNQPVFSLPSDTNTSERLSVSDSRVSSH 180
           F+LWMVE  NG           +G  DH+    +  + +    +      SV+D R+   
Sbjct: 129 FVLWMVERMNGERRNSPGGAQTDGNNDHTRETPSPCLHNRHHRSQPEPSRSVNDHRIPRD 188

Query: 181 GIE---RRRLFFYRHLDCLVHLIFKFLLVAIFLLIVVFVIPGSALVLLLYLLITLLFALP 240
            I+   R+ L F+      V L  KF LV IFLLI+++ IP SA +L +Y+L+TLL ALP
Sbjct: 189 NIQTSLRKSLVFF------VQLTAKFPLVVIFLLIILYAIPTSAAILAMYILVTLLLALP 248

Query: 241 SLLIFYLAVHALEKLMSDITS 248
           S LI Y A   L+ L+ +I +
Sbjct: 249 SFLILYFAYPCLDWLVREIVT 263

[Close]

BLAST of Lag0003986 vs. TAIR 10
Match: AT1G74370.1 (RING/U-box superfamily protein )

HSP 1 Score: 166.0 bits (419), Expect = 3.8e-41
Identity = 107/263 (40.68%), Postives = 156/263 (59.32%), Query Frame = 0


Query: 1   MWKLASNAI-AGIRSKKNSTLSKQVFWECSDDEVCSEASRDEELECPICWESFNIVENIP 60
           MW LAS +I  G  SK     +K             + +++E+LECPICWESFN+VEN+P
Sbjct: 1   MWNLASKSIREGFISKGEEAATKPRRATLDRSGDGRKTTKEEKLECPICWESFNVVENVP 60

Query: 61  YVLWCGHSLCKNCVLGLQGS-VLKVASRQIRIPIFISCPWCHQLSLRVVYRGNLKFPSKN 120
           YVLWCGH++CK C+LGLQ + V+K ++   ++P F++CPWC+ LSLR+V  G ++FPSKN
Sbjct: 61  YVLWCGHTICKYCLLGLQRAIVIKSSALPFQLPFFVACPWCNILSLRLVCNGTIRFPSKN 120

Query: 121 FFLLWMVESFNG--NEGKLDHSFNGNNQPVFSLPSDTNTSERL---SVSDSRVSSHGIER 180
           F+LLWMVES NG  +E   D+    + Q       D  ++  L    + D+R   +G+ R
Sbjct: 121 FYLLWMVESMNGSRSEAPSDNKRVASGQRDLRNRCDGVSNTALGDEGLLDNRSWWNGVTR 180

Query: 181 RRLFF---------YRHLDCLVHLIFKFLLVAIFLLIVVFVIPGSALVLLLYLLITLLFA 240
              FF          + +  + HL+ KF LV IFLL+ ++ IP SA VL +Y  +T   A
Sbjct: 181 G--FFRTGRLHDSVRKSMALVAHLLAKFPLVVIFLLMALYAIPVSAAVLGVYFFVTFALA 240

Query: 241 LPSLLIFYLAVHALEKLMSDITS 248
           +PS L+ Y A  +L  L+ +I +
Sbjct: 241 VPSFLVLYFAFPSLNWLIREIAT 261

[Close]

BLAST of Lag0003986 vs. TAIR 10
Match: AT1G21651.1 (zinc ion binding )

HSP 1 Score: 45.8 bits (107), Expect = 5.7e-05
Identity = 21/59 (35.59%), Postives = 30/59 (50.85%), Query Frame = 0


Query: 41  EELECPICWESFNIVENIPYVLWCGHSLCKNCVLGLQGSVLKVASRQIRIPIFISCPWC 100
           EE ECP+C +S++    +P VL CGH+ C+ C+  L            + P  I CP C
Sbjct: 2   EEPECPVCLQSYDGESTVPRVLACGHTACEECLTNLPK----------KFPDTIRCPAC 50

The following BLAST results are available for this feature:

BLAST of Lag0003986 vs. NCBI nr


Match Name	E-value	Identity	Description
KAG7029990.1	1.7e-112	84.74	hypothetical protein SDJN02_08334, partial [Cucurbita argyrosperma subsp. argyro...	[more]
KAG6599045.1	6.6e-112	84.34	hypothetical protein SDJN03_08823, partial [Cucurbita argyrosperma subsp. sorori...	[more]
XP_023547098.1	8.6e-112	83.94	uncharacterized protein LOC111806010 [Cucurbita pepo subsp. pepo]	[more]
XP_022999337.1	1.5e-111	84.34	uncharacterized protein LOC111493743 [Cucurbita maxima]	[more]
XP_022946373.1	1.9e-111	83.94	uncharacterized protein LOC111450453 [Cucurbita moschata]	[more]

BLAST of Lag0003986 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
Q9H0X6	6.6e-06	40.30	RING finger protein 208 OS=Homo sapiens OX=9606 GN=RNF208 PE=1 SV=2	[more]
Q8K0W3	1.1e-05	40.98	RING finger protein 208 OS=Mus musculus OX=10090 GN=Rnf208 PE=1 SV=2	[more]
Q96D59	1.2e-04	34.78	E3 ubiquitin-protein ligase RNF183 OS=Homo sapiens OX=9606 GN=RNF183 PE=1 SV=2	[more]
Q13049	2.1e-04	34.92	E3 ubiquitin-protein ligase TRIM32 OS=Homo sapiens OX=9606 GN=TRIM32 PE=1 SV=2	[more]
Q8CH72	2.1e-04	34.92	E3 ubiquitin-protein ligase TRIM32 OS=Mus musculus OX=10090 GN=Trim32 PE=1 SV=2	[more]

BLAST of Lag0003986 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A6J1KAL8	7.1e-112	84.34	uncharacterized protein LOC111493743 OS=Cucurbita maxima OX=3661 GN=LOC111493743...	[more]
A0A6J1G3L5	9.3e-112	83.94	uncharacterized protein LOC111450453 OS=Cucurbita moschata OX=3662 GN=LOC1114504...	[more]
A0A1S3CPH8	2.5e-109	83.81	uncharacterized protein LOC103503329 OS=Cucumis melo OX=3656 GN=LOC103503329 PE=...	[more]
A0A5A7TGX0	2.5e-109	83.81	Zf-RING_LisH domain-containing protein OS=Cucumis melo var. makuwa OX=1194695 GN...	[more]
A0A6J1D940	2.5e-109	84.46	uncharacterized protein LOC111018502 OS=Momordica charantia OX=3673 GN=LOC111018...	[more]

BLAST of Lag0003986 vs. TAIR 10


Match Name	E-value	Identity	Description
AT1G69330.1	8.2e-60	50.91	RING/U-box superfamily protein	[more]
AT3G29270.1	3.5e-55	45.98	RING/U-box superfamily protein	[more]
AT3G29270.2	3.5e-55	45.98	RING/U-box superfamily protein	[more]
AT1G74370.1	3.8e-41	40.68	RING/U-box superfamily protein	[more]
AT1G21651.1	5.7e-05	35.59	zinc ion binding	[more]

InterPro

Analysis Name: InterPro Annotations of Sponge gourd (AG-4) v1
Date Performed: 2022-08-01


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR013083	Zinc finger, RING/FYVE/PHD-type	GENE3D	3.30.40.10	Zinc/RING finger domain, C3HC4 (zinc finger)	coord: 33..137 e-value: 8.1E-13 score: 49.7
IPR027370	RING-type zinc-finger, LisH dimerisation motif	PFAM	PF13445	zf-RING_UBOX	coord: 45..74 e-value: 8.9E-8 score: 32.0
None	No IPR available	PANTHER	PTHR46616:SF1	TRANSCRIPTION FACTOR C2H2 FAMILY-RELATED	coord: 1..247
None	No IPR available	PANTHER	PTHR46616	UBIQUITIN-PROTEIN LIGASE	coord: 1..247
None	No IPR available	SUPERFAMILY	57850	RING/U-box	coord: 40..102
IPR017907	Zinc finger, RING-type, conserved site	PROSITE	PS00518	ZF_RING_1	coord: 64..73
IPR001841	Zinc finger, RING-type	PROSITE	PS50089	ZF_RING_2	coord: 45..100 score: 9.907503

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
Lag0003986.1	Lag0003986.1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0016567	protein ubiquitination
cellular_component	GO:0016021	integral component of membrane
molecular_function	GO:0046872	metal ion binding
molecular_function	GO:0004842	ubiquitin-protein transferase activity