Lag0003223 (gene) Sponge gourd (AG‐4) v1

BLAST of Lag0003223 vs. NCBI nr
Match: XP_008462190.1 (PREDICTED: auxin-responsive protein SAUR71-like [Cucumis melo] >KAA0059328.1 auxin-responsive protein SAUR71-like [Cucumis melo var. makuwa] >TYK03999.1 auxin-responsive protein SAUR71-like [Cucumis melo var. makuwa])

HSP 1 Score: 211.8 bits (538), Expect = 4.6e-51
Identity = 112/166 (67.47%), Postives = 132/166 (79.52%), Query Frame = 0

Query: 12  DYPKKSNKIRDIVRLQQILKKWKRLANGQKSSSNSNTCCSSNSNKGIKFLKTS-LSFTEA 71
           ++PKKSNKIR+IV+LQQI+KKWKRLANG+KS+S+SN           K LKTS + FT+ 
Sbjct: 5   NWPKKSNKIREIVKLQQIVKKWKRLANGEKSNSSSNN----------KLLKTSGVWFTDG 64

Query: 72  VPKGYLAVCVGKELKRFVIPTHYLTHQAFRMLLREAEEEFGFQQEGVLQIPCDVSVFENI 131
           VPKGYLAVCVGKE+KRFVIPTHYLTH+AFR+LL+EAEEEFGF Q+GVLQIPC VSVFE+I
Sbjct: 65  VPKGYLAVCVGKEMKRFVIPTHYLTHKAFRILLQEAEEEFGFHQQGVLQIPCHVSVFEDI 124

Query: 132 LNTVQHNHQPLFFQNH-PAPDNEIIGLSCSPDCELRPHHH-PQMCT 175
           LN VQ  +      NH  + DNEII L CSPDC+L  HHH PQ+CT
Sbjct: 125 LNIVQQQN-----DNHFASDDNEIIRLCCSPDCDLTHHHHPPQICT 155

BLAST of Lag0003223 vs. NCBI nr
Match: XP_038899517.1 (auxin-responsive protein SAUR71-like [Benincasa hispida])

HSP 1 Score: 207.6 bits (527), Expect = 8.6e-50
Identity = 114/166 (68.67%), Postives = 127/166 (76.51%), Query Frame = 0

Query: 12  DYPKKSNKIRDIVRLQQILKKWKRLANGQKSSSNSNTCCSSNSNKGIKFLKTS-LSFTEA 71
           D PKKSNKIR+IVRLQQI+KKWKRLANG+KSSSN +           K LK S +  T+ 
Sbjct: 9   DCPKKSNKIREIVRLQQIVKKWKRLANGEKSSSNKS-----------KLLKISGVWLTDV 68

Query: 72  VPKGYLAVCVGKELKRFVIPTHYLTHQAFRMLLREAEEEFGFQQEGVLQIPCDVSVFENI 131
           VPKGYLAVCVGKELKRFVIPTHYLTHQAFR+LL+EAEEEFGF Q+GVLQIPC VSVFE+I
Sbjct: 69  VPKGYLAVCVGKELKRFVIPTHYLTHQAFRILLQEAEEEFGFHQQGVLQIPCHVSVFEDI 128

Query: 132 LNTVQHNHQPLFFQNHPAP-DNEIIGLSCSPDCELRPHH-HPQMCT 175
           LNTVQ  +      N  AP DNEIIG  CSPDC L  HH  P++CT
Sbjct: 129 LNTVQQQN-----HNQFAPSDNEIIGFFCSPDCNLTHHHPPPRICT 158

BLAST of Lag0003223 vs. NCBI nr
Match: XP_004141833.2 (auxin-responsive protein SAUR40 [Cucumis sativus])

HSP 1 Score: 203.8 bits (517), Expect = 1.2e-48
Identity = 112/174 (64.37%), Postives = 130/174 (74.71%), Query Frame = 0

Query: 5   ESAKCVMDYPKKSNKIRDIVRLQQILKKWKRLANGQKSSSNSNTCCSSNSNKGIKFLKTS 64
           ES  C    PKKSNKIR+IV+LQQI+KKWKRLANG+KS+S+SN       NK +K     
Sbjct: 53  ESKNC----PKKSNKIREIVKLQQIVKKWKRLANGEKSNSSSN-------NKLLKI--NG 112

Query: 65  LSFTEAVPKGYLAVCVGKELKRFVIPTHYLTHQAFRMLLREAEEEFGFQQEGVLQIPCDV 124
             FT+ VPKGYLAVCVGKE+KRFVIPTHYLTH+AFR+LL+EAEEEFGF Q+GVLQIPC V
Sbjct: 113 AWFTDGVPKGYLAVCVGKEMKRFVIPTHYLTHKAFRILLQEAEEEFGFHQQGVLQIPCHV 172

Query: 125 SVFENILNTVQ---HNHQPLFFQNHPAPDNEIIGLSCSPDCELRPHH-HPQMCT 175
           SVFE+ILNTVQ   HNH         + DNEII   CSP+C+L  HH  PQ+CT
Sbjct: 173 SVFEDILNTVQQQNHNH-------FASDDNEIIRFCCSPECDLTHHHPPPQICT 206

BLAST of Lag0003223 vs. NCBI nr
Match: KGN45404.1 (hypothetical protein Csa_015702 [Cucumis sativus])

HSP 1 Score: 203.8 bits (517), Expect = 1.2e-48
Identity = 112/174 (64.37%), Postives = 130/174 (74.71%), Query Frame = 0

Query: 5   ESAKCVMDYPKKSNKIRDIVRLQQILKKWKRLANGQKSSSNSNTCCSSNSNKGIKFLKTS 64
           ES  C    PKKSNKIR+IV+LQQI+KKWKRLANG+KS+S+SN       NK +K     
Sbjct: 2   ESKNC----PKKSNKIREIVKLQQIVKKWKRLANGEKSNSSSN-------NKLLKI--NG 61

Query: 65  LSFTEAVPKGYLAVCVGKELKRFVIPTHYLTHQAFRMLLREAEEEFGFQQEGVLQIPCDV 124
             FT+ VPKGYLAVCVGKE+KRFVIPTHYLTH+AFR+LL+EAEEEFGF Q+GVLQIPC V
Sbjct: 62  AWFTDGVPKGYLAVCVGKEMKRFVIPTHYLTHKAFRILLQEAEEEFGFHQQGVLQIPCHV 121

Query: 125 SVFENILNTVQ---HNHQPLFFQNHPAPDNEIIGLSCSPDCELRPHH-HPQMCT 175
           SVFE+ILNTVQ   HNH         + DNEII   CSP+C+L  HH  PQ+CT
Sbjct: 122 SVFEDILNTVQQQNHNH-------FASDDNEIIRFCCSPECDLTHHHPPPQICT 155

BLAST of Lag0003223 vs. NCBI nr
Match: XP_002316430.1 (auxin-responsive protein SAUR72 [Populus trichocarpa] >PNT18667.1 hypothetical protein POPTR_010G253800 [Populus trichocarpa])

HSP 1 Score: 201.1 bits (510), Expect = 8.1e-48
Identity = 111/184 (60.33%), Postives = 130/184 (70.65%), Query Frame = 0

Query: 11  MDYPKKSNKIRDIVRLQQILKKWKRLANGQK----SSSNSNTCCSSNSNKGIKFLKTSLS 70
           MD+PKKSNKI DIVRLQQILKKW++ AN  K    SS+N+++  SSN++K IKFLK +LS
Sbjct: 1   MDHPKKSNKISDIVRLQQILKKWRKAANAPKNISSSSNNNSSSSSSNASKSIKFLKRTLS 60

Query: 71  FT-----------EAVPKGYLAVCVGKELKRFVIPTHYLTHQAFRMLLREAEEEFGFQQE 130
           FT           +AVPKGYLAVCVGKELKR++IPT YL HQAF +LLREAEEEFGFQQE
Sbjct: 61  FTDLSSSAAASSNDAVPKGYLAVCVGKELKRYIIPTEYLGHQAFGILLREAEEEFGFQQE 120

Query: 131 GVLQIPCDVSVFENILNTVQHNHQPLFFQ-----NHPAPDNEIIG-LSCSPDCELRPHHH 174
           GVL+IPC+V VFE IL  V+              N  +   E+IG  S SPDCEL P HH
Sbjct: 121 GVLKIPCEVPVFEKILKVVEEKKDVYLLHELGPVNAESTAKEMIGCYSQSPDCELTPSHH 180

BLAST of Lag0003223 vs. ExPASy Swiss-Prot
Match: Q9LTV3 (Auxin-responsive protein SAUR72 OS=Arabidopsis thaliana OX=3702 GN=SAUR72 PE=1 SV=1)

HSP 1 Score: 70.9 bits (172), Expect = 1.7e-11
Identity = 41/116 (35.34%), Postives = 73/116 (62.93%), Query Frame = 0

Query: 26  LQQILKKWKRLA-NGQKSSSNSNTCCSSNSNKGIKFLKTSLS-----FTEAVPKGYLAVC 85
           ++Q++++  R+A + Q S   S++     S +   FL++S++      T +VP+G++ V 
Sbjct: 1   MKQLIRRLSRVADSAQYSLLRSDS--QRPSRRSESFLRSSVTRRSKKQTSSVPEGHVPVY 60

Query: 86  VGKELKRFVIPTHYLTHQAFRMLLREAEEEFGFQQEGVLQIPCDVSVFENILNTVQ 136
           VG E++RFV+    L H  F  LL  + +E+G++Q+GVLQIPC V VFE I+ +++
Sbjct: 61  VGDEMERFVVSAELLNHPVFIGLLNRSAQEYGYEQKGVLQIPCHVLVFERIMESLR 114

BLAST of Lag0003223 vs. ExPASy Swiss-Prot
Match: O65695 (Auxin-responsive protein SAUR50 OS=Arabidopsis thaliana OX=3702 GN=SAUR50 PE=1 SV=1)

HSP 1 Score: 69.7 bits (169), Expect = 3.7e-11
Identity = 41/121 (33.88%), Postives = 63/121 (52.07%), Query Frame = 0

Query: 15  KKSNKIRDIVRLQQILKKWKRLANGQKSSSNSNTCCSSNSNKGIKFLKTSLSFTEAVPKG 74
           KK++K+     L+QILK+   L  G+K+    +  C                    VPKG
Sbjct: 5   KKTSKLTQTAMLKQILKRCSSL--GKKNGGGYDEDC----------------LPLDVPKG 64

Query: 75  YLAVCVGKELKRFVIPTHYLTHQAFRMLLREAEEEFGFQQEGVLQIPCDVSVFENILNTV 134
           +  V VG+   R+++P  +LTH  F+ LL+ AEEEFGF  +  L IPCD  VF+ + + +
Sbjct: 65  HFPVYVGENRSRYIVPISFLTHPEFQSLLQRAEEEFGFDHDMGLTIPCDELVFQTLTSMI 107

Query: 135 Q 136
           +
Sbjct: 125 R 107

BLAST of Lag0003223 vs. ExPASy Swiss-Prot
Match: P33081 (Auxin-induced protein 15A OS=Glycine max OX=3847 PE=2 SV=1)

HSP 1 Score: 68.2 bits (165), Expect = 1.1e-10
Identity = 35/60 (58.33%), Postives = 42/60 (70.00%), Query Frame = 0

Query: 72  PKGYLAVCVGKELKRFVIPTHYLTHQAFRMLLREAEEEFGFQQE-GVLQIPCDVSVFENI 131
           PKGYLAV VG++LKRFVIP  YL   +F+ LL +AEEEFG+    G L IPC   VF+ I
Sbjct: 18  PKGYLAVYVGEKLKRFVIPVSYLNQPSFQDLLSQAEEEFGYDHPMGGLTIPCSEDVFQCI 77

BLAST of Lag0003223 vs. ExPASy Swiss-Prot
Match: Q9SGU2 (Auxin-responsive protein SAUR71 OS=Arabidopsis thaliana OX=3702 GN=SAUR71 PE=2 SV=1)

HSP 1 Score: 68.2 bits (165), Expect = 1.1e-10
Identity = 38/110 (34.55%), Postives = 68/110 (61.82%), Query Frame = 0

Query: 26  LQQILKKWKRLANGQKSSSNSNTCCSSNSNKGIKFLKTSLSFTEAVPKGYLAVCVGKELK 85
           ++Q++++  R+A+     S   +   S S +G    +   S+   VP+G++ V VG E++
Sbjct: 1   MKQLIRRLSRVAD-----STQYSLLRSESQRGRTKKEKHKSW---VPEGHVPVYVGHEME 60

Query: 86  RFVIPTHYLTHQAFRMLLREAEEEFGFQQEGVLQIPCDVSVFENILNTVQ 136
           RFV+    L H  F  LL+++ +E+G++Q+GVL+IPC V VFE IL +++
Sbjct: 61  RFVVNAELLNHPVFVALLKQSAQEYGYEQQGVLRIPCHVLVFERILESLR 102

BLAST of Lag0003223 vs. ExPASy Swiss-Prot
Match: P33082 (Auxin-induced protein X15 OS=Glycine max OX=3847 PE=2 SV=1)

HSP 1 Score: 67.8 bits (164), Expect = 1.4e-10
Identity = 33/60 (55.00%), Postives = 42/60 (70.00%), Query Frame = 0

Query: 72  PKGYLAVCVGKELKRFVIPTHYLTHQAFRMLLREAEEEFGFQQE-GVLQIPCDVSVFENI 131
           PKGYLAV VG+++KRFVIP  Y+   +F+ LL +AEEEFG+    G L IPC   VF+ I
Sbjct: 18  PKGYLAVYVGEKMKRFVIPVSYMNQPSFQDLLTQAEEEFGYDHPMGGLTIPCSEEVFQRI 77