Lag0003177 (gene) Sponge gourd (AG‐4) v1

BLAST of Lag0003177 vs. NCBI nr
Match: XP_038897092.1 (GDSL lipase-like [Benincasa hispida])

HSP 1 Score: 649.4 bits (1674), Expect = 1.8e-182
Identity = 308/372 (82.80%), Postives = 335/372 (90.05%), Query Frame = 0

Query: 1   MKISNFHFFFSIFITIFLIAQSSQIADVPSSKKHVAFFVFGDSFFDPGNNNFINTSEGFR 60
           MKISNFHF F IF ++FLIA+SS+I DVP S+  +AFF+FGDSFFDPGNNNFINT+E FR
Sbjct: 1   MKISNFHFHFLIFSSLFLIARSSRIDDVPPSENRLAFFIFGDSFFDPGNNNFINTTEDFR 60

Query: 61  ANFTPYGESFFKDPTGRFSNGRLMPDFIAEYADLPQIPAYLDPHNNLYIHGVNFASGGGG 120
           ANFTPYGESFFK PTGRFS+GRL+PDFIAEYA+LP IPAYLDP N LYIHGVNFASGGGG
Sbjct: 61  ANFTPYGESFFKCPTGRFSDGRLIPDFIAEYANLPLIPAYLDPRNKLYIHGVNFASGGGG 120

Query: 121 ALVETHQGFVIDIETQLRYFKKVERSLKKKLGDSGARTLLSDSVYMFSIGGNDYMVAFDG 180
           ALVETH+GF IDIETQLRYFKKVERS++KKLGDS A  L  +SVYMFSIGGNDY+V F+G
Sbjct: 121 ALVETHRGFAIDIETQLRYFKKVERSMRKKLGDSRAYNLFLNSVYMFSIGGNDYIVPFEG 180

Query: 181 TPVLEKYTETEYVNMVIGNVTIVLEEIYKKGGRKFAFVAVPPLGCLPRTRLMKPDGHGSC 240
           +PVLEKYTETEYVNMVIGN T VLEEIYKKGGRKFAF AVPPLGCLP  RLMK  GHGSC
Sbjct: 181 SPVLEKYTETEYVNMVIGNTTAVLEEIYKKGGRKFAFAAVPPLGCLPHIRLMKKGGHGSC 240

Query: 241 WDEASALARLHNKVLPGALQKLADKLQGFKYTLADTYHMLQNRIDNPSKYGFKDGITGCC 300
           WDEASAL RLHNK+LP ALQKLADKLQGFKYT+ADTY +LQNRIDNPSKYGFK+G   CC
Sbjct: 241 WDEASALTRLHNKLLPIALQKLADKLQGFKYTVADTYTLLQNRIDNPSKYGFKEGKKACC 300

Query: 301 GSGEFRGVYTCGGMRGQKEFELCENPDEYLFFDSFHPNEKAYEQFAKLMWNGDTQVIKPY 360
           GSGE RG+Y+CGGMRGQKEFELCENP+EYLFFDS+HPNEKAYEQFAKLMW+GD QVIKPY
Sbjct: 301 GSGELRGIYSCGGMRGQKEFELCENPNEYLFFDSYHPNEKAYEQFAKLMWSGDAQVIKPY 360

Query: 361 NLKQLFQSGSLQ 373
           NLKQLFQ G  Q
Sbjct: 361 NLKQLFQCGLSQ 372

BLAST of Lag0003177 vs. NCBI nr
Match: XP_022964041.1 (GDSL esterase/lipase 5-like [Cucurbita moschata])

HSP 1 Score: 646.7 bits (1667), Expect = 1.2e-181
Identity = 307/372 (82.53%), Postives = 331/372 (88.98%), Query Frame = 0

Query: 1   MKISNFHFFFSIFITIFLIAQSSQIADVPSSKKHVAFFVFGDSFFDPGNNNFINTSEGFR 60
           MKISN HFFF IF TI   +  SQI DVPSS   VAFFVFGDSF DPGNNNFINT+E FR
Sbjct: 5   MKISNVHFFFKIFFTILFTSHGSQIDDVPSSNNGVAFFVFGDSFLDPGNNNFINTTEDFR 64

Query: 61  ANFTPYGESFFKDPTGRFSNGRLMPDFIAEYADLPQIPAYLDPHNNLYIHGVNFASGGGG 120
           ANFTPYGE+FF  PTGRFS+GRL+PDFIAEYA+LP IPAYLDPHNNLYIHGVNFASGGGG
Sbjct: 65  ANFTPYGETFFNSPTGRFSDGRLIPDFIAEYANLPLIPAYLDPHNNLYIHGVNFASGGGG 124

Query: 121 ALVETHQGFVIDIETQLRYFKKVERSLKKKLGDSGARTLLSDSVYMFSIGGNDYMVAFDG 180
           ALVETHQGF I+IETQLRYFKKVERSL+KKLGD+ A +LLSDSVYMFSIGGNDY+VAF+G
Sbjct: 125 ALVETHQGFAINIETQLRYFKKVERSLRKKLGDARASSLLSDSVYMFSIGGNDYIVAFEG 184

Query: 181 TPVLEKYTETEYVNMVIGNVTIVLEEIYKKGGRKFAFVAVPPLGCLPRTRLMKPDGHGSC 240
           +PVLEKYTE EYVNMVIGNVT VL+EIYKKGGRKFAF+AVPPLGC+P TRLMK  GHGSC
Sbjct: 185 SPVLEKYTEIEYVNMVIGNVTSVLQEIYKKGGRKFAFLAVPPLGCMPHTRLMKVGGHGSC 244

Query: 241 WDEASALARLHNKVLPGALQKLADKLQGFKYTLADTYHMLQNRIDNPSKYGFKDGITGCC 300
           WDEASALARLHNK+LP ALQKLA  LQGFKYTLADTY+MLQNRIDNPSKYG KDG T CC
Sbjct: 245 WDEASALARLHNKLLPAALQKLAHDLQGFKYTLADTYNMLQNRIDNPSKYGLKDGRTACC 304

Query: 301 GSGEFRGVYTCGGMRGQKEFELCENPDEYLFFDSFHPNEKAYEQFAKLMWNGDTQVIKPY 360
           GSGE RG+Y+CGGMRGQ EFELCENP+EYLFFDS+HPNE+AYEQFAKL+W GD Q I P 
Sbjct: 305 GSGELRGIYSCGGMRGQTEFELCENPNEYLFFDSYHPNERAYEQFAKLIWYGDAQFINPS 364

Query: 361 NLKQLFQSGSLQ 373
           NLKQ FQ GS Q
Sbjct: 365 NLKQFFQCGSSQ 376

BLAST of Lag0003177 vs. NCBI nr
Match: XP_023515160.1 (GDSL esterase/lipase 5-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 644.4 bits (1661), Expect = 5.9e-181
Identity = 304/372 (81.72%), Postives = 330/372 (88.71%), Query Frame = 0

Query: 1   MKISNFHFFFSIFITIFLIAQSSQIADVPSSKKHVAFFVFGDSFFDPGNNNFINTSEGFR 60
           MKISN HFFF IF TI   +  SQI D+PSS   VAFFVFGDS  DPGNNNFINT+E FR
Sbjct: 5   MKISNVHFFFKIFFTILFTSHGSQIGDIPSSNNGVAFFVFGDSLLDPGNNNFINTTEDFR 64

Query: 61  ANFTPYGESFFKDPTGRFSNGRLMPDFIAEYADLPQIPAYLDPHNNLYIHGVNFASGGGG 120
           ANFTPYGE+FF  PTGRFS+GRL+PDFIAEYA+LP IPAYLDPHNNLYIHGVNFASGGGG
Sbjct: 65  ANFTPYGETFFNSPTGRFSDGRLIPDFIAEYANLPLIPAYLDPHNNLYIHGVNFASGGGG 124

Query: 121 ALVETHQGFVIDIETQLRYFKKVERSLKKKLGDSGARTLLSDSVYMFSIGGNDYMVAFDG 180
           ALVETHQGF I+IETQLRYFKKVERSL+KKLGD+ A +LLSDSVYMFSIGGNDY+VAF+G
Sbjct: 125 ALVETHQGFAINIETQLRYFKKVERSLRKKLGDARASSLLSDSVYMFSIGGNDYIVAFEG 184

Query: 181 TPVLEKYTETEYVNMVIGNVTIVLEEIYKKGGRKFAFVAVPPLGCLPRTRLMKPDGHGSC 240
           +PVLEKYTE EYVNMVIGNVT VL+EIYKKGGRKFAF+AVPPLGC+P TRLMK  GHGSC
Sbjct: 185 SPVLEKYTEIEYVNMVIGNVTSVLQEIYKKGGRKFAFLAVPPLGCMPHTRLMKVGGHGSC 244

Query: 241 WDEASALARLHNKVLPGALQKLADKLQGFKYTLADTYHMLQNRIDNPSKYGFKDGITGCC 300
           WDEASALARLHNK+LP ALQKLA  LQGFKYTLADTY+MLQ RIDNPSKYG KDG   CC
Sbjct: 245 WDEASALARLHNKLLPAALQKLAHDLQGFKYTLADTYNMLQTRIDNPSKYGLKDGRRACC 304

Query: 301 GSGEFRGVYTCGGMRGQKEFELCENPDEYLFFDSFHPNEKAYEQFAKLMWNGDTQVIKPY 360
           GSGE RG+Y+CGGMRGQ+EFELCENP+EYLFFDS+HPNE+AYEQFAKL+WNGD Q I P 
Sbjct: 305 GSGELRGIYSCGGMRGQREFELCENPNEYLFFDSYHPNERAYEQFAKLIWNGDAQFINPS 364

Query: 361 NLKQLFQSGSLQ 373
           NLKQ FQ GS Q
Sbjct: 365 NLKQFFQCGSSQ 376

BLAST of Lag0003177 vs. NCBI nr
Match: XP_023000252.1 (GDSL esterase/lipase 5-like [Cucurbita maxima])

HSP 1 Score: 641.0 bits (1652), Expect = 6.5e-180
Identity = 303/372 (81.45%), Postives = 330/372 (88.71%), Query Frame = 0

Query: 1   MKISNFHFFFSIFITIFLIAQSSQIADVPSSKKHVAFFVFGDSFFDPGNNNFINTSEGFR 60
           MKISN HFFF IF TI   +  SQI DVPSS   VAFFVFGDS  DPGNNNFINT+E FR
Sbjct: 1   MKISNVHFFFKIFFTILFTSHGSQIGDVPSSHNGVAFFVFGDSLLDPGNNNFINTTEDFR 60

Query: 61  ANFTPYGESFFKDPTGRFSNGRLMPDFIAEYADLPQIPAYLDPHNNLYIHGVNFASGGGG 120
           ANFTPYGE+FF  P GRFS+GRL+PDFIAEYA+LP IPAYLDPH+NLYIHGVNFASGGGG
Sbjct: 61  ANFTPYGETFFNSPIGRFSDGRLIPDFIAEYANLPLIPAYLDPHSNLYIHGVNFASGGGG 120

Query: 121 ALVETHQGFVIDIETQLRYFKKVERSLKKKLGDSGARTLLSDSVYMFSIGGNDYMVAFDG 180
           ALVETHQGF I+IETQLRYFKKVERSL+KKLG + A +LLSDSVYMFSIGGNDY+VAF+G
Sbjct: 121 ALVETHQGFAINIETQLRYFKKVERSLRKKLGGARASSLLSDSVYMFSIGGNDYIVAFEG 180

Query: 181 TPVLEKYTETEYVNMVIGNVTIVLEEIYKKGGRKFAFVAVPPLGCLPRTRLMKPDGHGSC 240
           +PVLEK+TE EYVNMVIGNVT VL+EIYKKGGRKFAF+AVPPLGC+P TRLMK  GHGSC
Sbjct: 181 SPVLEKFTEIEYVNMVIGNVTSVLQEIYKKGGRKFAFLAVPPLGCMPHTRLMKVGGHGSC 240

Query: 241 WDEASALARLHNKVLPGALQKLADKLQGFKYTLADTYHMLQNRIDNPSKYGFKDGITGCC 300
           WDEASALARLHNK+LP ALQKL   LQGFKYTLADTY+MLQNRIDNPSKYGFKDG T CC
Sbjct: 241 WDEASALARLHNKLLPAALQKLTHDLQGFKYTLADTYNMLQNRIDNPSKYGFKDGRTACC 300

Query: 301 GSGEFRGVYTCGGMRGQKEFELCENPDEYLFFDSFHPNEKAYEQFAKLMWNGDTQVIKPY 360
           GSGE RG+Y+CGGMRGQ+EFELCENP+EYLFFDS+HPNE+AYEQFAKL+WNGD Q I P 
Sbjct: 301 GSGELRGIYSCGGMRGQREFELCENPNEYLFFDSYHPNERAYEQFAKLIWNGDDQFINPS 360

Query: 361 NLKQLFQSGSLQ 373
           NLKQ FQ GS Q
Sbjct: 361 NLKQFFQCGSSQ 372

BLAST of Lag0003177 vs. NCBI nr
Match: XP_008462253.1 (PREDICTED: GDSL esterase/lipase 5-like isoform X1 [Cucumis melo] >KAA0059365.1 GDSL esterase/lipase 5-like isoform X1 [Cucumis melo var. makuwa] >TYK03961.1 GDSL esterase/lipase 5-like isoform X1 [Cucumis melo var. makuwa])

HSP 1 Score: 624.8 bits (1610), Expect = 4.9e-175
Identity = 294/367 (80.11%), Postives = 326/367 (88.83%), Query Frame = 0

Query: 1   MKISNFHFFFSIFITIFLIAQSSQIADVPSSKKHVAFFVFGDSFFDPGNNNFINTSEGFR 60
           MKIS+ HFFF IF  +F IAQSS+I DV SS+K +AFF+FGDSFFDPGNNNFINT+E FR
Sbjct: 1   MKISSVHFFFLIFSAVFFIAQSSRIDDVSSSEKRLAFFIFGDSFFDPGNNNFINTTEDFR 60

Query: 61  ANFTPYGESFFKDPTGRFSNGRLMPDFIAEYADLPQIPAYLDPHNNLYIHGVNFASGGGG 120
           ANFTPYG+SFF+ PTGRFS+ RL+PDF+AEYA+LP IPAYLDP N  YIHGVNFASGGGG
Sbjct: 61  ANFTPYGKSFFRTPTGRFSDSRLVPDFVAEYANLPLIPAYLDPRNKRYIHGVNFASGGGG 120

Query: 121 ALVETHQGFVIDIETQLRYFKKVERSLKKKLGDSGARTLLSDSVYMFSIGGNDYMVAFDG 180
           ALVETH+GF IDIETQLRYFKKVERS++KKLGDS A  L S+SVY+FSIGGNDY+V F+G
Sbjct: 121 ALVETHRGFAIDIETQLRYFKKVERSIRKKLGDSRAYNLFSNSVYLFSIGGNDYIVPFEG 180

Query: 181 TPVLEKYTETEYVNMVIGNVTIVLEEIYKKGGRKFAFVAVPPLGCLPRTRLMKPDGHGSC 240
           +PV EKYTETEYVNMVIGNVT VLEEIYKKGGRKFAFVAVPPLGCLP  RLMK DGHGSC
Sbjct: 181 SPVFEKYTETEYVNMVIGNVTAVLEEIYKKGGRKFAFVAVPPLGCLPHIRLMKKDGHGSC 240

Query: 241 WDEASALARLHNKVLPGALQKLADKLQGFKYTLADTYHMLQNRIDNPSKYGFKDGITGCC 300
            DEASAL RLHNK+L  ALQKLADKL GFKYT+ DTY MLQNRIDNPSKYG K+G   CC
Sbjct: 241 LDEASALVRLHNKLLHAALQKLADKLHGFKYTIGDTYTMLQNRIDNPSKYGLKEGKKACC 300

Query: 301 GSGEFRGVYTCGGMRGQKEFELCENPDEYLFFDSFHPNEKAYEQFAKLMWNGDTQVIKPY 360
           GSG+ RG+Y+CGGMRG KEFELCENP+EYLFFDS+HPNE+AYEQFAKLMW+GD+QVI PY
Sbjct: 301 GSGKLRGIYSCGGMRGVKEFELCENPNEYLFFDSYHPNERAYEQFAKLMWSGDSQVINPY 360

Query: 361 NLKQLFQ 368
           NLKQ FQ
Sbjct: 361 NLKQFFQ 367

BLAST of Lag0003177 vs. ExPASy Swiss-Prot
Match: H6U1I8 (GDSL lipase OS=Tanacetum cinerariifolium OX=118510 GN=GLIP PE=1 SV=1)

HSP 1 Score: 370.2 bits (949), Expect = 2.8e-101
Identity = 180/338 (53.25%), Postives = 231/338 (68.34%), Query Frame = 0

Query: 30  SSKKHVAFFVFGDSFFDPGNNNFINTSEGFRANFTPYGESFFKDPTGRFSNGRLMPDFIA 89
           SS++  A F+FGDS FDPGNNN INT   F+ANF PYG+S+F  PTGRFS+GR++PDFIA
Sbjct: 27  SSQQAAALFIFGDSVFDPGNNNHINTHVNFKANFWPYGQSYFSSPTGRFSDGRIIPDFIA 86

Query: 90  EYADLPQIPAYLDPHNNLYIHGVNFASGGGGALVETHQGFVIDIETQLRYFKKVERSLKK 149
           EYA LP IPAYL+P NN + HG NFAS G GAL+ +H G  + ++TQLRYF  +    ++
Sbjct: 87  EYASLPIIPAYLEP-NNDFTHGANFASAGAGALIASHAGLAVGLQTQLRYFGDLVDHYRQ 146

Query: 150 KLGDSGARTLLSDSVYMFSIGGNDYMVAFDGTPVLEKYTETEYVNMVIGNVTIVLEEIYK 209
            LGD  +R LLSD+VY+FS GGNDY   +        YT+ +YV++VIGN+T V++ IY+
Sbjct: 147 NLGDIKSRQLLSDAVYLFSCGGNDYQSPY------YPYTQEQYVDIVIGNMTNVIKGIYE 206

Query: 210 KGGRKFAFVAVPPLGCLPRTRLMKPDGHGSCWDEASALARLHNKVLPGALQKLADKLQGF 269
           KGGRKF  V VP +GC P  R  +P    +C  E   L RLHN+     L++L  +L+GF
Sbjct: 207 KGGRKFGVVNVPLIGCWPGMRAKQPG--NTCNTEVDELTRLHNQAFAKRLEQLEKQLEGF 266

Query: 270 KYTLADTYHMLQNRIDNPSKYGFKDGITGCCGSGEFRGVYTCGGMRGQKEFELCENPDEY 329
            Y   D    + NR+ NPSKYGFK+G + CCGSG F G Y CG +   KEF LC+N  EY
Sbjct: 267 VYAKFDLSTAILNRMKNPSKYGFKEGESACCGSGPFGGNYDCGRI---KEFGLCDNATEY 326

Query: 330 LFFDSFHPNEKAYEQFAKLMWNGDTQVIKPYNLKQLFQ 368
            FFD FHPNE A  QFA++ W+GD+ V +PYNLK LF+
Sbjct: 327 FFFDPFHPNELASRQFAEMFWDGDSMVTQPYNLKALFE 352

BLAST of Lag0003177 vs. ExPASy Swiss-Prot
Match: Q9SSA7 (GDSL esterase/lipase 5 OS=Arabidopsis thaliana OX=3702 GN=GLIP5 PE=2 SV=2)

HSP 1 Score: 357.1 bits (915), Expect = 2.5e-97
Identity = 179/370 (48.38%), Postives = 248/370 (67.03%), Query Frame = 0

Query: 3   ISNFHFFFSIFITIFLIAQSSQIADVPSSKKHVAFFVFGDSFFDPGNNNFINTSEGFRAN 62
           IS+F FF      +FL  +SS            A F+FGDSF D GNNN+INT+   +AN
Sbjct: 15  ISSFIFFIVSSTILFLAGKSSAKISHNGDNNVTALFLFGDSFLDAGNNNYINTTTLDQAN 74

Query: 63  FTPYGESFFKDPTGRFSNGRLMPDFIAEYADLPQIPAYLDPHNN-LYIHGVNFASGGGGA 122
           F PYG++FF  PTGRFS+GRL+ DFIAEYA+LP IP +L+P N+   ++GVNFAS G GA
Sbjct: 75  FPPYGQTFFGLPTGRFSDGRLISDFIAEYANLPLIPPFLEPGNSQKKLYGVNFASAGAGA 134

Query: 123 LVETHQGFVIDIETQLRYFKKVERSLKKKLGDSGARTLLSDSVYMFSIGGNDYMVAFDGT 182
           LVET QG VI++ TQL ++KKVER  +   G   ++  +S +VY+ SIG NDY   F   
Sbjct: 135 LVETFQGSVINLRTQLDHYKKVERLWRTNFGKEESKKRISRAVYLISIGSNDYSSIFLTN 194

Query: 183 PVLEKYTETEYVNMVIGNVTIVLEEIYKKGGRKFAFVAVPPLGCLPRTRLMKPDGHGSCW 242
             L   + +++V++VIGN+T  + EIYK GGRKF F+ VP LGC P  R+++P    SC 
Sbjct: 195 QSL-PISMSQHVDIVIGNLTTFIHEIYKIGGRKFGFLNVPDLGCFPALRILQPKNDDSCL 254

Query: 243 DEASALARLHNKVLPGALQKLADKLQGFKYTLADTYHMLQNRIDNPSKYGFKDGITGCCG 302
            +AS LA +HN+ L   L ++  +++GFK++L D    L+ R+ +PSK+GFK+G   CCG
Sbjct: 255 RDASRLASMHNRALTNLLFQMQRQVKGFKFSLFDMNKSLRLRMQHPSKFGFKEGEEACCG 314

Query: 303 SGEFRGVYTCGGMRGQKEFELCENPDEYLFFDSFHPNEKAYEQFAKLMWNG----DTQVI 362
           +G++RGV++CGG R  KE++LCENP +Y+F+DS H  +  Y QFA L+WNG    D+ V+
Sbjct: 315 TGKWRGVFSCGGKRIVKEYQLCENPKDYIFWDSLHLTQNTYNQFANLIWNGGHMSDSLVV 374

Query: 363 KPYNLKQLFQ 368
            PYN+  LFQ
Sbjct: 375 GPYNINNLFQ 383

BLAST of Lag0003177 vs. ExPASy Swiss-Prot
Match: Q9SYF0 (GDSL esterase/lipase 2 OS=Arabidopsis thaliana OX=3702 GN=GLIP2 PE=2 SV=1)

HSP 1 Score: 342.0 bits (876), Expect = 8.2e-93
Identity = 166/336 (49.40%), Postives = 227/336 (67.56%), Query Frame = 0

Query: 36  AFFVFGDSFFDPGNNNFINTSEGFRANFTPYGESFFKDPTGRFSNGRLMPDFIAEYADLP 95
           A FVFGDS FD GNNN+I+T   FR+N+ PYG++ FK PTGR S+GR +PDFIAEYA LP
Sbjct: 39  ALFVFGDSVFDAGNNNYIDTLPSFRSNYWPYGQTTFKFPTGRVSDGRTIPDFIAEYAWLP 98

Query: 96  QIPAYLDPHN--NLYIHGVNFASGGGGALVETHQGFVIDIETQLRYFKKVERSLKKKLGD 155
            IPAYL P N  N + +GV+FAS G GALV T  G VI++++QL  FKKVE+ L+  LG+
Sbjct: 99  LIPAYLQPSNGKNQFPYGVSFASAGAGALVGTFPGMVINLKSQLNNFKKVEKLLRSTLGE 158

Query: 156 SGARTLLSDSVYMFSIGGNDYMVAFD-GTPVLEKYTETEYVNMVIGNVTIVLEEIYKKGG 215
           +  + ++S +VY+F IG NDY   F   + + +   +  YV+ V+GN T V++E+YK GG
Sbjct: 159 AQGKMVISRAVYLFHIGVNDYQYPFSTNSSIFQSSPQEIYVDFVVGNTTAVIKEVYKIGG 218

Query: 216 RKFAFVAVPPLGCLPRTRLMKPDGHGSCWDEASALARLHNKVLPGALQKLADKLQGFKYT 275
           RKF F+ +    C P + ++     G+C+   + L  LHN+ L   L++L  +L GFKY 
Sbjct: 219 RKFGFLNMGAYDCAPASLIIDQTKIGTCFKPVTELINLHNEKLESGLRRLERELSGFKYA 278

Query: 276 LADTYHMLQNRIDNPSKYGFKDGITGCCGSGEFRGVYTCGGMRG-QKEFELCENPDEYLF 335
           L D +  L  R++NPSKYGFK+G   CCG+G  RG+ TCGG  G  + +ELCE   +YLF
Sbjct: 279 LHDYHTSLSVRMNNPSKYGFKEGKMACCGTGPLRGINTCGGRMGVSQSYELCEKVTDYLF 338

Query: 336 FDSFHPNEKAYEQFAKLMWNGDTQVIKPYNLKQLFQ 368
           FD FH  EKA++Q A+L+W+G T V KPYNL+ LF+
Sbjct: 339 FDHFHLTEKAHQQIAELIWSGPTNVTKPYNLQALFE 374

BLAST of Lag0003177 vs. ExPASy Swiss-Prot
Match: Q9FLN0 (GDSL esterase/lipase 1 OS=Arabidopsis thaliana OX=3702 GN=GLIP1 PE=1 SV=1)

HSP 1 Score: 338.6 bits (867), Expect = 9.1e-92
Identity = 166/336 (49.40%), Postives = 221/336 (65.77%), Query Frame = 0

Query: 36  AFFVFGDSFFDPGNNNFINTSEGFRANFTPYGESFFKDPTGRFSNGRLMPDFIAEYADLP 95
           A FVFGDS FD GNNN+I+T    R+N+ PYG++ FK PTGR S+GRL+PDFIAEYA LP
Sbjct: 37  ALFVFGDSVFDAGNNNYIDTLSSVRSNYWPYGQTTFKSPTGRVSDGRLIPDFIAEYAWLP 96

Query: 96  QIPAYLDPH--NNLYIHGVNFASGGGGALVETHQGFVIDIETQLRYFKKVERSLKKKLGD 155
            IP  L P   N+ + +GVNFASGG GALV T  G VI++ TQL  FKKVE  L+ KLGD
Sbjct: 97  LIPPNLQPFNGNSQFAYGVNFASGGAGALVGTFSGLVINLRTQLNNFKKVEEMLRSKLGD 156

Query: 156 SGARTLLSDSVYMFSIGGNDYMVAF-DGTPVLEKYTETEYVNMVIGNVTIVLEEIYKKGG 215
           +  + ++S +VY+F IG NDY   F   + + +  +  +YV+ V+GN+T V +E+Y  GG
Sbjct: 157 AEGKRVISRAVYLFHIGLNDYQYPFTTNSSLFQSISNEKYVDYVVGNMTDVFKEVYNLGG 216

Query: 216 RKFAFVAVPPLGCLPRTRLMKPDGHGSCWDEASALARLHNKVLPGALQKLADKLQGFKYT 275
           RKF  +   P  C P + ++      SC+   + L  +HN+ L   L++L  +L GFKY 
Sbjct: 217 RKFGILNTGPYDCAPASLVIDQTKIRSCFQPVTELINMHNEKLLNGLRRLNHELSGFKYA 276

Query: 276 LADTYHMLQNRIDNPSKYGFKDGITGCCGSGEFRGVYTCGGMRG-QKEFELCENPDEYLF 335
           L D +  L  R+++PSKYGFK+G   CCGSG  RG+ TCGG  G  + +ELCEN  +YLF
Sbjct: 277 LHDYHTSLSERMNDPSKYGFKEGKKACCGSGPLRGINTCGGRMGLSQSYELCENVTDYLF 336

Query: 336 FDSFHPNEKAYEQFAKLMWNGDTQVIKPYNLKQLFQ 368
           FD FH  EKA  Q A+L+W+G T +  PYNLK LF+
Sbjct: 337 FDPFHLTEKANRQIAELIWSGPTNITGPYNLKALFE 372

BLAST of Lag0003177 vs. ExPASy Swiss-Prot
Match: Q9LJP1 (GDSL esterase/lipase 4 OS=Arabidopsis thaliana OX=3702 GN=GLIP4 PE=2 SV=2)

HSP 1 Score: 326.2 bits (835), Expect = 4.7e-88
Identity = 166/371 (44.74%), Postives = 226/371 (60.92%), Query Frame = 0

Query: 9   FFSIFITIFLIAQSSQIADVPSSK----KHVAFFVFGDSFFDPGNNNFINTSEGFRANFT 68
           F SI I +F+   S  I  +   +       A F FGDS F+ GNNN+ ++   FR+NF 
Sbjct: 6   FNSIIIILFICTISLSIVSISCKEDLKTNQAALFAFGDSLFEAGNNNYFDSISSFRSNFW 65

Query: 69  PYGESFFKDPTGRFSNGRLMPDFIAEYADLPQIPAYLDP--HNNLYIHGVNFASGGGGAL 128
           PYG++ FK PTGR S+GR+M DFIAEYA LP IP  L P   N+   +G+NFA+   G  
Sbjct: 66  PYGKTTFKFPTGRVSDGRIMIDFIAEYAWLPLIPPNLQPGYSNSQLTYGLNFATTAAGVF 125

Query: 129 VETHQGFVI----DIETQLRYFKKVERSLKKKLGDSGARTLLSDSVYMFSIGGNDYMVA- 188
             T  G V     D+ TQL  FK VE++L+  LGD+ AR ++S +VY+F IG NDY    
Sbjct: 126 AGTFPGSVTNLSKDLGTQLNNFKNVEKTLRSNLGDAEARRVISKAVYLFHIGANDYQYPF 185

Query: 189 FDGTPVLEKYTETEYVNMVIGNVTIVLEEIYKKGGRKFAFVAVPPLGCLPRTRLMKPDGH 248
           F  T      T+  +++ VIGN T V+EE+YK G RKF F+++ P GC P   ++     
Sbjct: 186 FANTSTFSNTTKERFIDFVIGNTTTVIEELYKLGARKFGFLSLGPFGCTPSALIINSTKI 245

Query: 249 GSCWDEASALARLHNKVLPGALQKLADKLQGFKYTLADTYHMLQNRIDNPSKYGFKDGIT 308
           GSC++  + L  LHN+  P  L++L  +L GFKY L D +  L  RI+NPS+YGFK+G  
Sbjct: 246 GSCFEPVTELINLHNQEFPKVLRRLERRLSGFKYALHDFHTSLSQRINNPSRYGFKEGEM 305

Query: 309 GCCGSGEFRGVYTCGGMRGQKE-FELCENPDEYLFFDSFHPNEKAYEQFAKLMWNGDTQV 368
            CCGSG  RG+ TCG   G  + ++LCEN D+Y+FFD  H  E A++Q A+L+W+G   V
Sbjct: 306 ACCGSGPLRGINTCGFRNGPSQGYKLCENADDYVFFDPSHLTETAHQQIAELIWSGPPNV 365

BLAST of Lag0003177 vs. ExPASy TrEMBL
Match: A0A6J1HHR5 (GDSL esterase/lipase 5-like OS=Cucurbita moschata OX=3662 GN=LOC111464186 PE=3 SV=1)

HSP 1 Score: 646.7 bits (1667), Expect = 5.8e-182
Identity = 307/372 (82.53%), Postives = 331/372 (88.98%), Query Frame = 0

Query: 1   MKISNFHFFFSIFITIFLIAQSSQIADVPSSKKHVAFFVFGDSFFDPGNNNFINTSEGFR 60
           MKISN HFFF IF TI   +  SQI DVPSS   VAFFVFGDSF DPGNNNFINT+E FR
Sbjct: 5   MKISNVHFFFKIFFTILFTSHGSQIDDVPSSNNGVAFFVFGDSFLDPGNNNFINTTEDFR 64

Query: 61  ANFTPYGESFFKDPTGRFSNGRLMPDFIAEYADLPQIPAYLDPHNNLYIHGVNFASGGGG 120
           ANFTPYGE+FF  PTGRFS+GRL+PDFIAEYA+LP IPAYLDPHNNLYIHGVNFASGGGG
Sbjct: 65  ANFTPYGETFFNSPTGRFSDGRLIPDFIAEYANLPLIPAYLDPHNNLYIHGVNFASGGGG 124

Query: 121 ALVETHQGFVIDIETQLRYFKKVERSLKKKLGDSGARTLLSDSVYMFSIGGNDYMVAFDG 180
           ALVETHQGF I+IETQLRYFKKVERSL+KKLGD+ A +LLSDSVYMFSIGGNDY+VAF+G
Sbjct: 125 ALVETHQGFAINIETQLRYFKKVERSLRKKLGDARASSLLSDSVYMFSIGGNDYIVAFEG 184

Query: 181 TPVLEKYTETEYVNMVIGNVTIVLEEIYKKGGRKFAFVAVPPLGCLPRTRLMKPDGHGSC 240
           +PVLEKYTE EYVNMVIGNVT VL+EIYKKGGRKFAF+AVPPLGC+P TRLMK  GHGSC
Sbjct: 185 SPVLEKYTEIEYVNMVIGNVTSVLQEIYKKGGRKFAFLAVPPLGCMPHTRLMKVGGHGSC 244

Query: 241 WDEASALARLHNKVLPGALQKLADKLQGFKYTLADTYHMLQNRIDNPSKYGFKDGITGCC 300
           WDEASALARLHNK+LP ALQKLA  LQGFKYTLADTY+MLQNRIDNPSKYG KDG T CC
Sbjct: 245 WDEASALARLHNKLLPAALQKLAHDLQGFKYTLADTYNMLQNRIDNPSKYGLKDGRTACC 304

Query: 301 GSGEFRGVYTCGGMRGQKEFELCENPDEYLFFDSFHPNEKAYEQFAKLMWNGDTQVIKPY 360
           GSGE RG+Y+CGGMRGQ EFELCENP+EYLFFDS+HPNE+AYEQFAKL+W GD Q I P 
Sbjct: 305 GSGELRGIYSCGGMRGQTEFELCENPNEYLFFDSYHPNERAYEQFAKLIWYGDAQFINPS 364

Query: 361 NLKQLFQSGSLQ 373
           NLKQ FQ GS Q
Sbjct: 365 NLKQFFQCGSSQ 376

BLAST of Lag0003177 vs. ExPASy TrEMBL
Match: A0A6J1KD54 (GDSL esterase/lipase 5-like OS=Cucurbita maxima OX=3661 GN=LOC111494533 PE=3 SV=1)

HSP 1 Score: 641.0 bits (1652), Expect = 3.2e-180
Identity = 303/372 (81.45%), Postives = 330/372 (88.71%), Query Frame = 0

Query: 1   MKISNFHFFFSIFITIFLIAQSSQIADVPSSKKHVAFFVFGDSFFDPGNNNFINTSEGFR 60
           MKISN HFFF IF TI   +  SQI DVPSS   VAFFVFGDS  DPGNNNFINT+E FR
Sbjct: 1   MKISNVHFFFKIFFTILFTSHGSQIGDVPSSHNGVAFFVFGDSLLDPGNNNFINTTEDFR 60

Query: 61  ANFTPYGESFFKDPTGRFSNGRLMPDFIAEYADLPQIPAYLDPHNNLYIHGVNFASGGGG 120
           ANFTPYGE+FF  P GRFS+GRL+PDFIAEYA+LP IPAYLDPH+NLYIHGVNFASGGGG
Sbjct: 61  ANFTPYGETFFNSPIGRFSDGRLIPDFIAEYANLPLIPAYLDPHSNLYIHGVNFASGGGG 120

Query: 121 ALVETHQGFVIDIETQLRYFKKVERSLKKKLGDSGARTLLSDSVYMFSIGGNDYMVAFDG 180
           ALVETHQGF I+IETQLRYFKKVERSL+KKLG + A +LLSDSVYMFSIGGNDY+VAF+G
Sbjct: 121 ALVETHQGFAINIETQLRYFKKVERSLRKKLGGARASSLLSDSVYMFSIGGNDYIVAFEG 180

Query: 181 TPVLEKYTETEYVNMVIGNVTIVLEEIYKKGGRKFAFVAVPPLGCLPRTRLMKPDGHGSC 240
           +PVLEK+TE EYVNMVIGNVT VL+EIYKKGGRKFAF+AVPPLGC+P TRLMK  GHGSC
Sbjct: 181 SPVLEKFTEIEYVNMVIGNVTSVLQEIYKKGGRKFAFLAVPPLGCMPHTRLMKVGGHGSC 240

Query: 241 WDEASALARLHNKVLPGALQKLADKLQGFKYTLADTYHMLQNRIDNPSKYGFKDGITGCC 300
           WDEASALARLHNK+LP ALQKL   LQGFKYTLADTY+MLQNRIDNPSKYGFKDG T CC
Sbjct: 241 WDEASALARLHNKLLPAALQKLTHDLQGFKYTLADTYNMLQNRIDNPSKYGFKDGRTACC 300

Query: 301 GSGEFRGVYTCGGMRGQKEFELCENPDEYLFFDSFHPNEKAYEQFAKLMWNGDTQVIKPY 360
           GSGE RG+Y+CGGMRGQ+EFELCENP+EYLFFDS+HPNE+AYEQFAKL+WNGD Q I P 
Sbjct: 301 GSGELRGIYSCGGMRGQREFELCENPNEYLFFDSYHPNERAYEQFAKLIWNGDDQFINPS 360

Query: 361 NLKQLFQSGSLQ 373
           NLKQ FQ GS Q
Sbjct: 361 NLKQFFQCGSSQ 372

BLAST of Lag0003177 vs. ExPASy TrEMBL
Match: A0A5A7V0K2 (GDSL esterase/lipase 5-like isoform X1 OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold347G001580 PE=3 SV=1)

HSP 1 Score: 624.8 bits (1610), Expect = 2.3e-175
Identity = 294/367 (80.11%), Postives = 326/367 (88.83%), Query Frame = 0

Query: 1   MKISNFHFFFSIFITIFLIAQSSQIADVPSSKKHVAFFVFGDSFFDPGNNNFINTSEGFR 60
           MKIS+ HFFF IF  +F IAQSS+I DV SS+K +AFF+FGDSFFDPGNNNFINT+E FR
Sbjct: 1   MKISSVHFFFLIFSAVFFIAQSSRIDDVSSSEKRLAFFIFGDSFFDPGNNNFINTTEDFR 60

Query: 61  ANFTPYGESFFKDPTGRFSNGRLMPDFIAEYADLPQIPAYLDPHNNLYIHGVNFASGGGG 120
           ANFTPYG+SFF+ PTGRFS+ RL+PDF+AEYA+LP IPAYLDP N  YIHGVNFASGGGG
Sbjct: 61  ANFTPYGKSFFRTPTGRFSDSRLVPDFVAEYANLPLIPAYLDPRNKRYIHGVNFASGGGG 120

Query: 121 ALVETHQGFVIDIETQLRYFKKVERSLKKKLGDSGARTLLSDSVYMFSIGGNDYMVAFDG 180
           ALVETH+GF IDIETQLRYFKKVERS++KKLGDS A  L S+SVY+FSIGGNDY+V F+G
Sbjct: 121 ALVETHRGFAIDIETQLRYFKKVERSIRKKLGDSRAYNLFSNSVYLFSIGGNDYIVPFEG 180

Query: 181 TPVLEKYTETEYVNMVIGNVTIVLEEIYKKGGRKFAFVAVPPLGCLPRTRLMKPDGHGSC 240
           +PV EKYTETEYVNMVIGNVT VLEEIYKKGGRKFAFVAVPPLGCLP  RLMK DGHGSC
Sbjct: 181 SPVFEKYTETEYVNMVIGNVTAVLEEIYKKGGRKFAFVAVPPLGCLPHIRLMKKDGHGSC 240

Query: 241 WDEASALARLHNKVLPGALQKLADKLQGFKYTLADTYHMLQNRIDNPSKYGFKDGITGCC 300
            DEASAL RLHNK+L  ALQKLADKL GFKYT+ DTY MLQNRIDNPSKYG K+G   CC
Sbjct: 241 LDEASALVRLHNKLLHAALQKLADKLHGFKYTIGDTYTMLQNRIDNPSKYGLKEGKKACC 300

Query: 301 GSGEFRGVYTCGGMRGQKEFELCENPDEYLFFDSFHPNEKAYEQFAKLMWNGDTQVIKPY 360
           GSG+ RG+Y+CGGMRG KEFELCENP+EYLFFDS+HPNE+AYEQFAKLMW+GD+QVI PY
Sbjct: 301 GSGKLRGIYSCGGMRGVKEFELCENPNEYLFFDSYHPNERAYEQFAKLMWSGDSQVINPY 360

Query: 361 NLKQLFQ 368
           NLKQ FQ
Sbjct: 361 NLKQFFQ 367

BLAST of Lag0003177 vs. ExPASy TrEMBL
Match: A0A1S3CI17 (GDSL esterase/lipase 5-like isoform X1 OS=Cucumis melo OX=3656 GN=LOC103500655 PE=3 SV=1)

HSP 1 Score: 624.8 bits (1610), Expect = 2.3e-175
Identity = 294/367 (80.11%), Postives = 326/367 (88.83%), Query Frame = 0

Query: 1   MKISNFHFFFSIFITIFLIAQSSQIADVPSSKKHVAFFVFGDSFFDPGNNNFINTSEGFR 60
           MKIS+ HFFF IF  +F IAQSS+I DV SS+K +AFF+FGDSFFDPGNNNFINT+E FR
Sbjct: 1   MKISSVHFFFLIFSAVFFIAQSSRIDDVSSSEKRLAFFIFGDSFFDPGNNNFINTTEDFR 60

Query: 61  ANFTPYGESFFKDPTGRFSNGRLMPDFIAEYADLPQIPAYLDPHNNLYIHGVNFASGGGG 120
           ANFTPYG+SFF+ PTGRFS+ RL+PDF+AEYA+LP IPAYLDP N  YIHGVNFASGGGG
Sbjct: 61  ANFTPYGKSFFRTPTGRFSDSRLVPDFVAEYANLPLIPAYLDPRNKRYIHGVNFASGGGG 120

Query: 121 ALVETHQGFVIDIETQLRYFKKVERSLKKKLGDSGARTLLSDSVYMFSIGGNDYMVAFDG 180
           ALVETH+GF IDIETQLRYFKKVERS++KKLGDS A  L S+SVY+FSIGGNDY+V F+G
Sbjct: 121 ALVETHRGFAIDIETQLRYFKKVERSIRKKLGDSRAYNLFSNSVYLFSIGGNDYIVPFEG 180

Query: 181 TPVLEKYTETEYVNMVIGNVTIVLEEIYKKGGRKFAFVAVPPLGCLPRTRLMKPDGHGSC 240
           +PV EKYTETEYVNMVIGNVT VLEEIYKKGGRKFAFVAVPPLGCLP  RLMK DGHGSC
Sbjct: 181 SPVFEKYTETEYVNMVIGNVTAVLEEIYKKGGRKFAFVAVPPLGCLPHIRLMKKDGHGSC 240

Query: 241 WDEASALARLHNKVLPGALQKLADKLQGFKYTLADTYHMLQNRIDNPSKYGFKDGITGCC 300
            DEASAL RLHNK+L  ALQKLADKL GFKYT+ DTY MLQNRIDNPSKYG K+G   CC
Sbjct: 241 LDEASALVRLHNKLLHAALQKLADKLHGFKYTIGDTYTMLQNRIDNPSKYGLKEGKKACC 300

Query: 301 GSGEFRGVYTCGGMRGQKEFELCENPDEYLFFDSFHPNEKAYEQFAKLMWNGDTQVIKPY 360
           GSG+ RG+Y+CGGMRG KEFELCENP+EYLFFDS+HPNE+AYEQFAKLMW+GD+QVI PY
Sbjct: 301 GSGKLRGIYSCGGMRGVKEFELCENPNEYLFFDSYHPNERAYEQFAKLMWSGDSQVINPY 360

Query: 361 NLKQLFQ 368
           NLKQ FQ
Sbjct: 361 NLKQFFQ 367

BLAST of Lag0003177 vs. ExPASy TrEMBL
Match: A0A0A0KCD4 (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_7G448130 PE=3 SV=1)

HSP 1 Score: 623.2 bits (1606), Expect = 6.8e-175
Identity = 294/373 (78.82%), Postives = 326/373 (87.40%), Query Frame = 0

Query: 1   MKISNFHFFFSIFITIFLIAQSSQIADVPSSKKHVAFFVFGDSFFDPGNNNFINTSEGFR 60
           MKI N HF F IF  +F IAQSS I DV S +K +AFF+FGDS FDPGNNNFINT+E FR
Sbjct: 1   MKIPNLHFLFLIFTAVFFIAQSSLIDDVSSPEKRLAFFIFGDSLFDPGNNNFINTTEDFR 60

Query: 61  ANFTPYGESFFKDPTGRFSNGRLMPDFIAEYADLPQIPAYLDPHNNLYIHGVNFASGGGG 120
           ANFTPYGESFFK PTGRFS+GRL+PDF+AEYA+LP IPAYLDPHN  YIHGVNFASGGGG
Sbjct: 61  ANFTPYGESFFKTPTGRFSDGRLVPDFVAEYANLPLIPAYLDPHNKRYIHGVNFASGGGG 120

Query: 121 ALVETHQGFVIDIETQLRYFKKVERSLKKKLGDSGARTLLSDSVYMFSIGGNDYMVAFDG 180
           ALVETH+GF IDIETQLRYFKKVERS++KKLGD  A  L S+SVY+FSIGGNDY+V F+G
Sbjct: 121 ALVETHRGFAIDIETQLRYFKKVERSIRKKLGDWRAYNLFSNSVYLFSIGGNDYIVPFEG 180

Query: 181 TPVLEKYTETEYVNMVIGNVTIVLEEIYKKGGRKFAFVAVPPLGCLPRTRLM-KPDGHGS 240
           +P+ +KYTE EYVNMVIGN T VLEEIYKKGGRKFAFVAVPPLGCLP  RL+ K  GHGS
Sbjct: 181 SPIFDKYTEREYVNMVIGNATAVLEEIYKKGGRKFAFVAVPPLGCLPHIRLVKKAGGHGS 240

Query: 241 CWDEASALARLHNKVLPGALQKLADKLQGFKYTLADTYHMLQNRIDNPSKYGFKDGITGC 300
           CWDE SAL RLHNK+LPGALQKLADKLQGFKYT+ DTY MLQNRIDNPSKYGFK+  T C
Sbjct: 241 CWDEPSALVRLHNKLLPGALQKLADKLQGFKYTVGDTYTMLQNRIDNPSKYGFKEEKTAC 300

Query: 301 CGSGEFRGVYTCGGMRGQKEFELCENPDEYLFFDSFHPNEKAYEQFAKLMWNGDTQVIKP 360
           CGSG+FRG+Y+CGGMRG KEFELCENP+EYLFFDS+HPNE+AYEQFAKLMW+GD+QVI P
Sbjct: 301 CGSGKFRGIYSCGGMRGVKEFELCENPNEYLFFDSYHPNERAYEQFAKLMWSGDSQVINP 360

Query: 361 YNLKQLFQSGSLQ 373
           Y+LKQ FQ  S Q
Sbjct: 361 YSLKQFFQYASSQ 373

BLAST of Lag0003177 vs. TAIR 10
Match: AT1G53920.1 (GDSL-motif lipase 5 )

HSP 1 Score: 357.1 bits (915), Expect = 1.8e-98
Identity = 179/370 (48.38%), Postives = 248/370 (67.03%), Query Frame = 0

Query: 3   ISNFHFFFSIFITIFLIAQSSQIADVPSSKKHVAFFVFGDSFFDPGNNNFINTSEGFRAN 62
           IS+F FF      +FL  +SS            A F+FGDSF D GNNN+INT+   +AN
Sbjct: 15  ISSFIFFIVSSTILFLAGKSSAKISHNGDNNVTALFLFGDSFLDAGNNNYINTTTLDQAN 74

Query: 63  FTPYGESFFKDPTGRFSNGRLMPDFIAEYADLPQIPAYLDPHNN-LYIHGVNFASGGGGA 122
           F PYG++FF  PTGRFS+GRL+ DFIAEYA+LP IP +L+P N+   ++GVNFAS G GA
Sbjct: 75  FPPYGQTFFGLPTGRFSDGRLISDFIAEYANLPLIPPFLEPGNSQKKLYGVNFASAGAGA 134

Query: 123 LVETHQGFVIDIETQLRYFKKVERSLKKKLGDSGARTLLSDSVYMFSIGGNDYMVAFDGT 182
           LVET QG VI++ TQL ++KKVER  +   G   ++  +S +VY+ SIG NDY   F   
Sbjct: 135 LVETFQGSVINLRTQLDHYKKVERLWRTNFGKEESKKRISRAVYLISIGSNDYSSIFLTN 194

Query: 183 PVLEKYTETEYVNMVIGNVTIVLEEIYKKGGRKFAFVAVPPLGCLPRTRLMKPDGHGSCW 242
             L   + +++V++VIGN+T  + EIYK GGRKF F+ VP LGC P  R+++P    SC 
Sbjct: 195 QSL-PISMSQHVDIVIGNLTTFIHEIYKIGGRKFGFLNVPDLGCFPALRILQPKNDDSCL 254

Query: 243 DEASALARLHNKVLPGALQKLADKLQGFKYTLADTYHMLQNRIDNPSKYGFKDGITGCCG 302
            +AS LA +HN+ L   L ++  +++GFK++L D    L+ R+ +PSK+GFK+G   CCG
Sbjct: 255 RDASRLASMHNRALTNLLFQMQRQVKGFKFSLFDMNKSLRLRMQHPSKFGFKEGEEACCG 314

Query: 303 SGEFRGVYTCGGMRGQKEFELCENPDEYLFFDSFHPNEKAYEQFAKLMWNG----DTQVI 362
           +G++RGV++CGG R  KE++LCENP +Y+F+DS H  +  Y QFA L+WNG    D+ V+
Sbjct: 315 TGKWRGVFSCGGKRIVKEYQLCENPKDYIFWDSLHLTQNTYNQFANLIWNGGHMSDSLVV 374

Query: 363 KPYNLKQLFQ 368
            PYN+  LFQ
Sbjct: 375 GPYNINNLFQ 383

BLAST of Lag0003177 vs. TAIR 10
Match: AT5G40990.1 (GDSL lipase 1 )

HSP 1 Score: 338.6 bits (867), Expect = 6.5e-93
Identity = 166/336 (49.40%), Postives = 221/336 (65.77%), Query Frame = 0

Query: 36  AFFVFGDSFFDPGNNNFINTSEGFRANFTPYGESFFKDPTGRFSNGRLMPDFIAEYADLP 95
           A FVFGDS FD GNNN+I+T    R+N+ PYG++ FK PTGR S+GRL+PDFIAEYA LP
Sbjct: 37  ALFVFGDSVFDAGNNNYIDTLSSVRSNYWPYGQTTFKSPTGRVSDGRLIPDFIAEYAWLP 96

Query: 96  QIPAYLDPH--NNLYIHGVNFASGGGGALVETHQGFVIDIETQLRYFKKVERSLKKKLGD 155
            IP  L P   N+ + +GVNFASGG GALV T  G VI++ TQL  FKKVE  L+ KLGD
Sbjct: 97  LIPPNLQPFNGNSQFAYGVNFASGGAGALVGTFSGLVINLRTQLNNFKKVEEMLRSKLGD 156

Query: 156 SGARTLLSDSVYMFSIGGNDYMVAF-DGTPVLEKYTETEYVNMVIGNVTIVLEEIYKKGG 215
           +  + ++S +VY+F IG NDY   F   + + +  +  +YV+ V+GN+T V +E+Y  GG
Sbjct: 157 AEGKRVISRAVYLFHIGLNDYQYPFTTNSSLFQSISNEKYVDYVVGNMTDVFKEVYNLGG 216

Query: 216 RKFAFVAVPPLGCLPRTRLMKPDGHGSCWDEASALARLHNKVLPGALQKLADKLQGFKYT 275
           RKF  +   P  C P + ++      SC+   + L  +HN+ L   L++L  +L GFKY 
Sbjct: 217 RKFGILNTGPYDCAPASLVIDQTKIRSCFQPVTELINMHNEKLLNGLRRLNHELSGFKYA 276

Query: 276 LADTYHMLQNRIDNPSKYGFKDGITGCCGSGEFRGVYTCGGMRG-QKEFELCENPDEYLF 335
           L D +  L  R+++PSKYGFK+G   CCGSG  RG+ TCGG  G  + +ELCEN  +YLF
Sbjct: 277 LHDYHTSLSERMNDPSKYGFKEGKKACCGSGPLRGINTCGGRMGLSQSYELCENVTDYLF 336

Query: 336 FDSFHPNEKAYEQFAKLMWNGDTQVIKPYNLKQLFQ 368
           FD FH  EKA  Q A+L+W+G T +  PYNLK LF+
Sbjct: 337 FDPFHLTEKANRQIAELIWSGPTNITGPYNLKALFE 372

BLAST of Lag0003177 vs. TAIR 10
Match: AT1G53940.1 (GDSL-motif lipase 2 )

HSP 1 Score: 327.8 bits (839), Expect = 1.1e-89
Identity = 161/342 (47.08%), Postives = 227/342 (66.37%), Query Frame = 0

Query: 36  AFFVFGDSFFDPGNNNFINTSEGFRANFTPYGESFFKDPTGRFSNGRLMPDFIAEYADLP 95
           A FVFGDS FD GNNN+I+T   FR+N+ PYG++ FK PTGR S+GR +PDFIAEYA LP
Sbjct: 39  ALFVFGDSVFDAGNNNYIDTLPSFRSNYWPYGQTTFKFPTGRVSDGRTIPDFIAEYAWLP 98

Query: 96  QIPAYLDPHN--NLYIHGVNFASGGGGALVETHQGFVIDIETQLRYFKKVERSLKKKLGD 155
            IPAYL P N  N + +GV+FAS G GALV T  G VI++++QL  FKKVE+ L+  LG+
Sbjct: 99  LIPAYLQPSNGKNQFPYGVSFASAGAGALVGTFPGMVINLKSQLNNFKKVEKLLRSTLGE 158

Query: 156 SGARTLLSDSVYMFSIGGNDYMVAFD-GTPVLEKYTETEYVNMVIGNVTIVLEEIYKKGG 215
           +  + ++S +VY+F IG NDY   F   + + +   +  YV+ V+GN T V++E+YK GG
Sbjct: 159 AQGKMVISRAVYLFHIGVNDYQYPFSTNSSIFQSSPQEIYVDFVVGNTTAVIKEVYKIGG 218

Query: 216 RKFAFVAVPPLGCLPRTRLMKPDGHGSCWDEASALARLHNKVLPGALQKLADKLQGFKYT 275
           RKF F+ +    C P + ++     G+C+   + L  LHN+ L   L++L  +L GFKY 
Sbjct: 219 RKFGFLNMGAYDCAPASLIIDQTKIGTCFKPVTELINLHNEKLESGLRRLERELSGFKYA 278

Query: 276 LADTYHMLQNRIDNPSKYGFKDGITGCCGSGEFRGVYTCGGMRG-QKEFELCENPDEYLF 335
           L D +  L  R++NPSKYGFK+G   CCG+G  RG+ TCGG  G  + +ELCE   +YLF
Sbjct: 279 LHDYHTSLSVRMNNPSKYGFKEGKMACCGTGPLRGINTCGGRMGVSQSYELCEKVTDYLF 338

Query: 336 FDSFHPNEKAYEQFAKLMWNGDTQVIKPYNLKQLFQSGSLQG 374
           FD FH  EKA++Q A+L+W+G T  ++   ++ +  +G L+G
Sbjct: 339 FDHFHLTEKAHQQIAELIWSGPTNGLRK-GIRHVCGTGPLRG 379

BLAST of Lag0003177 vs. TAIR 10
Match: AT3G14225.1 (GDSL-motif lipase 4 )

HSP 1 Score: 326.2 bits (835), Expect = 3.3e-89
Identity = 166/371 (44.74%), Postives = 226/371 (60.92%), Query Frame = 0

Query: 9   FFSIFITIFLIAQSSQIADVPSSK----KHVAFFVFGDSFFDPGNNNFINTSEGFRANFT 68
           F SI I +F+   S  I  +   +       A F FGDS F+ GNNN+ ++   FR+NF 
Sbjct: 6   FNSIIIILFICTISLSIVSISCKEDLKTNQAALFAFGDSLFEAGNNNYFDSISSFRSNFW 65

Query: 69  PYGESFFKDPTGRFSNGRLMPDFIAEYADLPQIPAYLDP--HNNLYIHGVNFASGGGGAL 128
           PYG++ FK PTGR S+GR+M DFIAEYA LP IP  L P   N+   +G+NFA+   G  
Sbjct: 66  PYGKTTFKFPTGRVSDGRIMIDFIAEYAWLPLIPPNLQPGYSNSQLTYGLNFATTAAGVF 125

Query: 129 VETHQGFVI----DIETQLRYFKKVERSLKKKLGDSGARTLLSDSVYMFSIGGNDYMVA- 188
             T  G V     D+ TQL  FK VE++L+  LGD+ AR ++S +VY+F IG NDY    
Sbjct: 126 AGTFPGSVTNLSKDLGTQLNNFKNVEKTLRSNLGDAEARRVISKAVYLFHIGANDYQYPF 185

Query: 189 FDGTPVLEKYTETEYVNMVIGNVTIVLEEIYKKGGRKFAFVAVPPLGCLPRTRLMKPDGH 248
           F  T      T+  +++ VIGN T V+EE+YK G RKF F+++ P GC P   ++     
Sbjct: 186 FANTSTFSNTTKERFIDFVIGNTTTVIEELYKLGARKFGFLSLGPFGCTPSALIINSTKI 245

Query: 249 GSCWDEASALARLHNKVLPGALQKLADKLQGFKYTLADTYHMLQNRIDNPSKYGFKDGIT 308
           GSC++  + L  LHN+  P  L++L  +L GFKY L D +  L  RI+NPS+YGFK+G  
Sbjct: 246 GSCFEPVTELINLHNQEFPKVLRRLERRLSGFKYALHDFHTSLSQRINNPSRYGFKEGEM 305

Query: 309 GCCGSGEFRGVYTCGGMRGQKE-FELCENPDEYLFFDSFHPNEKAYEQFAKLMWNGDTQV 368
            CCGSG  RG+ TCG   G  + ++LCEN D+Y+FFD  H  E A++Q A+L+W+G   V
Sbjct: 306 ACCGSGPLRGINTCGFRNGPSQGYKLCENADDYVFFDPSHLTETAHQQIAELIWSGPPNV 365

BLAST of Lag0003177 vs. TAIR 10
Match: AT1G53990.1 (GDSL-motif lipase 3 )

HSP 1 Score: 321.2 bits (822), Expect = 1.1e-87
Identity = 165/362 (45.58%), Postives = 223/362 (61.60%), Query Frame = 0

Query: 10  FSIFITIFLIAQSSQIADVPSSKKHVAFFVFGDSFFDPGNNNFINTSEGFRANFTPYGES 69
           F ++  I  I   + I +        A FVFGDS FD GNNN+INT   FR+N  PYG++
Sbjct: 9   FFVYTIILSIGSINCIDNNNLVTNQAALFVFGDSLFDAGNNNYINTVSSFRSNIWPYGQT 68

Query: 70  FFKDPTGRFSNGRLMPDFIAEYADLPQIPAYLDPH--NNLYIHGVNFASGGGGALVETHQ 129
            FK PTGR S+G        E A LP IP  L P+  NN + +GV+FAS G GAL E+  
Sbjct: 69  NFKFPTGRLSDG-------PEKAWLPSIPPNLQPNNGNNQFTYGVSFASAGAGALAESFL 128

Query: 130 GFVIDIETQLRYFKKVERSLKKKLGDSGARTLLSDSVYMFSIGGNDYMVAFD-GTPVLEK 189
           G VI++ TQL  FK VE+SL+ +LGD+  + + S +VY+F IG NDY   F   +   + 
Sbjct: 129 GMVINLGTQLNNFKDVEKSLRSELGDAETKRVFSRAVYLFHIGANDYFYPFSANSSTFKS 188

Query: 190 YTETEYVNMVIGNVTIVLEEIYKKGGRKFAFVAVPPLGCLPRTRLMKPDGHGSCWDEASA 249
            ++ ++V+ VIGN+T V+EE+YK GGRKF F+ V P  C P + +      GSC+   + 
Sbjct: 189 NSKEKFVDFVIGNITFVIEEVYKMGGRKFGFLNVGPYECSPNSLIRDRTKIGSCFKPVAE 248

Query: 250 LARLHNKVLPGALQKLADKLQGFKYTLADTYHMLQNRIDNPSKYGFKDGITGCCGSGEFR 309
           L  +HNK  P  L++L  +L GF+Y L D +  L  RI++PSKYGFK+G   CCGSG  R
Sbjct: 249 LIDMHNKKFPDVLRRLQRQLSGFRYALHDYHTSLSERINSPSKYGFKEGKKACCGSGPLR 308

Query: 310 GVYTCGGMRGQKE-FELCENPDEYLFFDSFHPNEKAYEQFAKLMWNGDTQVIKPYNLKQL 368
           G+ TCG   G  + + LCEN  +YLF+DS H  EKA+ Q A+L+WNG   V +PYNLK L
Sbjct: 309 GINTCGNRIGPSQGYGLCENVTDYLFYDSSHLTEKAHRQIAELIWNGPPNVTRPYNLKAL 363