Lag0003136 (gene) Sponge gourd (AG‐4) v1

Overview


Name	Lag0003136
Type	gene
Organism	Luffa acutangula (Sponge gourd (AG‐4) v1)
Description	Germin-like protein
Location	chr4: 48369978 .. 48370604 (+)
RNA-Seq Expression	Lag0003136
Synteny	Lag0003136

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonCDSpolypeptide

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGTTTCACTCCCTATTCATCATTTCCCTCCTCTTCTCCCTTTCCAATGCCGCCGTCCAGGACTTCTGCGTTGCTGATCTAAAAGGTCCACAAGGGCCAGCCGGCTACTCCTGCAAGAGCGCTGCTGACGTGACCGCAGATGACTTTGTGTATTCAGGCTTAATCGGTGCTGGAAACATATCAAGTCTAATCAAAGCTGCAGTCACCCCAGCATTTGTGGAGCAGTATCCAGGTGTGAATGGCCTCGGTCTCTCGGCCGCTCGGTTAGACTTGGCTCCAGGTGGTGTTATTCCACTTCACACACATCCAGGAGCTTCAGAATTGCTGCTTGTGACACAAGGAGTCATTGAGGCTGGCTTCATCTCTTCTGCTAATGCTGTCTACGTGAAGACTCTGAAAAAGGGAGACCTCATGATATTCCCACAAGGGTTGCTACACTTTCAGATTAATGCTGGTGGTGTGACTGCCGTTGGCTTTCCTATATTTAGCAGCCCAAGCCCTGGTCTCCAAATCACTGACTTTGCACTATTTGCCAACGACTTAAGTACTGATTTGGTGTCGAAGACCACTTTCCTGGACCCGGCTCTGGTGAAGAAGTTTAAGGCTGTTCTTGGTGGGAGTGGTTAG

mRNA sequence

ATGTTTCACTCCCTATTCATCATTTCCCTCCTCTTCTCCCTTTCCAATGCCGCCGTCCAGGACTTCTGCGTTGCTGATCTAAAAGGTCCACAAGGGCCAGCCGGCTACTCCTGCAAGAGCGCTGCTGACGTGACCGCAGATGACTTTGTGTATTCAGGCTTAATCGGTGCTGGAAACATATCAAGTCTAATCAAAGCTGCAGTCACCCCAGCATTTGTGGAGCAGTATCCAGGTGTGAATGGCCTCGGTCTCTCGGCCGCTCGGTTAGACTTGGCTCCAGGTGGTGTTATTCCACTTCACACACATCCAGGAGCTTCAGAATTGCTGCTTGTGACACAAGGAGTCATTGAGGCTGGCTTCATCTCTTCTGCTAATGCTGTCTACGTGAAGACTCTGAAAAAGGGAGACCTCATGATATTCCCACAAGGGTTGCTACACTTTCAGATTAATGCTGGTGGTGTGACTGCCGTTGGCTTTCCTATATTTAGCAGCCCAAGCCCTGGTCTCCAAATCACTGACTTTGCACTATTTGCCAACGACTTAAGTACTGATTTGGTGTCGAAGACCACTTTCCTGGACCCGGCTCTGGTGAAGAAGTTTAAGGCTGTTCTTGGTGGGAGTGGTTAG

Coding sequence (CDS)

ATGTTTCACTCCCTATTCATCATTTCCCTCCTCTTCTCCCTTTCCAATGCCGCCGTCCAGGACTTCTGCGTTGCTGATCTAAAAGGTCCACAAGGGCCAGCCGGCTACTCCTGCAAGAGCGCTGCTGACGTGACCGCAGATGACTTTGTGTATTCAGGCTTAATCGGTGCTGGAAACATATCAAGTCTAATCAAAGCTGCAGTCACCCCAGCATTTGTGGAGCAGTATCCAGGTGTGAATGGCCTCGGTCTCTCGGCCGCTCGGTTAGACTTGGCTCCAGGTGGTGTTATTCCACTTCACACACATCCAGGAGCTTCAGAATTGCTGCTTGTGACACAAGGAGTCATTGAGGCTGGCTTCATCTCTTCTGCTAATGCTGTCTACGTGAAGACTCTGAAAAAGGGAGACCTCATGATATTCCCACAAGGGTTGCTACACTTTCAGATTAATGCTGGTGGTGTGACTGCCGTTGGCTTTCCTATATTTAGCAGCCCAAGCCCTGGTCTCCAAATCACTGACTTTGCACTATTTGCCAACGACTTAAGTACTGATTTGGTGTCGAAGACCACTTTCCTGGACCCGGCTCTGGTGAAGAAGTTTAAGGCTGTTCTTGGTGGGAGTGGTTAG

Protein sequence

MFHSLFIISLLFSLSNAAVQDFCVADLKGPQGPAGYSCKSAADVTADDFVYSGLIGAGNISSLIKAAVTPAFVEQYPGVNGLGLSAARLDLAPGGVIPLHTHPGASELLLVTQGVIEAGFISSANAVYVKTLKKGDLMIFPQGLLHFQINAGGVTAVGFPIFSSPSPGLQITDFALFANDLSTDLVSKTTFLDPALVKKFKAVLGGSG

Homology

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BLAST of Lag0003136 vs. NCBI nr
Match: XP_038897216.1 (auxin-binding protein ABP19a [Benincasa hispida])

HSP 1 Score: 387.5 bits (994), Expect = 7.3e-104
Identity = 197/208 (94.71%), Postives = 200/208 (96.15%), Query Frame = 0


Query: 1   MFHSLFIISLLFSLSNAAVQDFCVADLKGPQGPAGYSCKSAADVTADDFVYSGLIGAGNI 60
           MFHSL IISLLFSL NAAVQDFCVADLKGPQGPAGYSCKSA  VTADDFVYSGL GAGNI
Sbjct: 1   MFHSLLIISLLFSLCNAAVQDFCVADLKGPQGPAGYSCKSAGVVTADDFVYSGLSGAGNI 60

Query: 61  SSLIKAAVTPAFVEQYPGVNGLGLSAARLDLAPGGVIPLHTHPGASELLLVTQGVIEAGF 120
           SSLIKAAVTPAFVEQYPGVNGLGLSAARLDLAPGGVIPLHTHPGASELLLVTQG+I+AGF
Sbjct: 61  SSLIKAAVTPAFVEQYPGVNGLGLSAARLDLAPGGVIPLHTHPGASELLLVTQGIIQAGF 120

Query: 121 ISSANAVYVKTLKKGDLMIFPQGLLHFQINAGGVTAVGFPIFSSPSPGLQITDFALFAND 180
           ISSANAVYVKTLKKGDLMIFPQGLLHFQIN GGVTAVGFPIFSSPSPGLQI DFALFAND
Sbjct: 121 ISSANAVYVKTLKKGDLMIFPQGLLHFQINGGGVTAVGFPIFSSPSPGLQILDFALFAND 180

Query: 181 LSTDLVSKTTFLDPALVKKFKAVLGGSG 209
           LSTDLVSKTTFLDPAL+KK KAVLGGSG
Sbjct: 181 LSTDLVSKTTFLDPALIKKLKAVLGGSG 208

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BLAST of Lag0003136 vs. NCBI nr
Match: XP_022964299.1 (auxin-binding protein ABP19a-like [Cucurbita moschata] >XP_023514157.1 auxin-binding protein ABP19a-like isoform X1 [Cucurbita pepo subsp. pepo] >XP_023514158.1 auxin-binding protein ABP19a-like isoform X2 [Cucurbita pepo subsp. pepo] >KAG6593548.1 Auxin-binding protein ABP19a, partial [Cucurbita argyrosperma subsp. sororia] >KAG7025890.1 Auxin-binding protein ABP19a, partial [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 384.4 bits (986), Expect = 6.2e-103
Identity = 193/208 (92.79%), Postives = 204/208 (98.08%), Query Frame = 0


Query: 1   MFHSLFIISLLFSLSNAAVQDFCVADLKGPQGPAGYSCKSAADVTADDFVYSGLIGAGNI 60
           MFHSLF+I+LLFSLSNAAVQDFCVADLKGPQGPAGY+CKSA  VTADDFVYSGL GAGNI
Sbjct: 1   MFHSLFVIALLFSLSNAAVQDFCVADLKGPQGPAGYACKSA--VTADDFVYSGLSGAGNI 60

Query: 61  SSLIKAAVTPAFVEQYPGVNGLGLSAARLDLAPGGVIPLHTHPGASELLLVTQGVIEAGF 120
           +SLIKAAVTPAFVEQYPGVNGLGLSAARLDL PGGVIPLHTHPGASELL+VTQG+I+AGF
Sbjct: 61  TSLIKAAVTPAFVEQYPGVNGLGLSAARLDLLPGGVIPLHTHPGASELLIVTQGIIQAGF 120

Query: 121 ISSANAVYVKTLKKGDLMIFPQGLLHFQINAGGVTAVGFPIFSSPSPGLQITDFALFAND 180
           ISSANAV+VKTLKKGDLMIFPQGLLHFQINAGGVTAVG+P+FSSPSPGLQITDFALFAND
Sbjct: 121 ISSANAVFVKTLKKGDLMIFPQGLLHFQINAGGVTAVGYPVFSSPSPGLQITDFALFAND 180

Query: 181 LSTDLVSKTTFLDPALVKKFKAVLGGSG 209
           LSTDLVSKTTFLDPALVKKFKA+LGGSG
Sbjct: 181 LSTDLVSKTTFLDPALVKKFKAILGGSG 206

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BLAST of Lag0003136 vs. NCBI nr
Match: KAA0059402.1 (auxin-binding protein ABP19a [Cucumis melo var. makuwa] >TYK03925.1 auxin-binding protein ABP19a [Cucumis melo var. makuwa])

HSP 1 Score: 384.0 bits (985), Expect = 8.0e-103
Identity = 194/208 (93.27%), Postives = 200/208 (96.15%), Query Frame = 0


Query: 1   MFHSLFIISLLFSLSNAAVQDFCVADLKGPQGPAGYSCKSAADVTADDFVYSGLIGAGNI 60
           MFHSL IISLLFSLSNAAVQDFCVADLKGPQGPAGYSCK A+ VTADDFVY+GL  AGNI
Sbjct: 1   MFHSLLIISLLFSLSNAAVQDFCVADLKGPQGPAGYSCKIASVVTADDFVYTGLRAAGNI 60

Query: 61  SSLIKAAVTPAFVEQYPGVNGLGLSAARLDLAPGGVIPLHTHPGASELLLVTQGVIEAGF 120
           SSLIKAAVTPAFVEQYPGVNGLGLSAARLDLAPGGVIPLHTHPGASELLLVTQG+IEAGF
Sbjct: 61  SSLIKAAVTPAFVEQYPGVNGLGLSAARLDLAPGGVIPLHTHPGASELLLVTQGIIEAGF 120

Query: 121 ISSANAVYVKTLKKGDLMIFPQGLLHFQINAGGVTAVGFPIFSSPSPGLQITDFALFAND 180
           ISSANAVYVKTLKKGDLM+FPQGLLHFQINAGGVTAVGFP+FSSPSPGLQI DFALFAND
Sbjct: 121 ISSANAVYVKTLKKGDLMVFPQGLLHFQINAGGVTAVGFPVFSSPSPGLQILDFALFAND 180

Query: 181 LSTDLVSKTTFLDPALVKKFKAVLGGSG 209
           LSTDLVSKTTFLDP L+KK KAVLGGSG
Sbjct: 181 LSTDLVSKTTFLDPVLIKKLKAVLGGSG 208

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BLAST of Lag0003136 vs. NCBI nr
Match: XP_004141699.1 (auxin-binding protein ABP19a [Cucumis sativus] >KGN45492.1 hypothetical protein Csa_016297 [Cucumis sativus])

HSP 1 Score: 381.7 bits (979), Expect = 4.0e-102
Identity = 193/208 (92.79%), Postives = 200/208 (96.15%), Query Frame = 0


Query: 1   MFHSLFIISLLFSLSNAAVQDFCVADLKGPQGPAGYSCKSAADVTADDFVYSGLIGAGNI 60
           M HSL IISLLFSLSNAAVQDFCVADLKGPQGPAGYSCK A+ VTADDFV+SGL  AGNI
Sbjct: 1   MLHSLLIISLLFSLSNAAVQDFCVADLKGPQGPAGYSCKIASVVTADDFVFSGLRAAGNI 60

Query: 61  SSLIKAAVTPAFVEQYPGVNGLGLSAARLDLAPGGVIPLHTHPGASELLLVTQGVIEAGF 120
           SSLIKAAVTPAFVEQYPGVNGLGLSAARLDLAPGGVIPLHTHPGASELLLVTQG+IEAGF
Sbjct: 61  SSLIKAAVTPAFVEQYPGVNGLGLSAARLDLAPGGVIPLHTHPGASELLLVTQGIIEAGF 120

Query: 121 ISSANAVYVKTLKKGDLMIFPQGLLHFQINAGGVTAVGFPIFSSPSPGLQITDFALFAND 180
           ISSANAVYVKTLKKGDLM+FPQGLLHFQINAGGVTAVGFP+FSSPSPGLQI DFALFAND
Sbjct: 121 ISSANAVYVKTLKKGDLMVFPQGLLHFQINAGGVTAVGFPVFSSPSPGLQILDFALFAND 180

Query: 181 LSTDLVSKTTFLDPALVKKFKAVLGGSG 209
           LSTDLVSKTTFLDPAL+KK K+VLGGSG
Sbjct: 181 LSTDLVSKTTFLDPALIKKLKSVLGGSG 208

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BLAST of Lag0003136 vs. NCBI nr
Match: XP_023000032.1 (auxin-binding protein ABP19a-like [Cucurbita maxima])

HSP 1 Score: 374.4 bits (960), Expect = 6.4e-100
Identity = 189/208 (90.87%), Postives = 200/208 (96.15%), Query Frame = 0


Query: 1   MFHSLFIISLLFSLSNAAVQDFCVADLKGPQGPAGYSCKSAADVTADDFVYSGLIGAGNI 60
           MFHSLF+I LLFSLSNAAVQDFCVADLKGPQGPAGY+CKSA  VTADDFVYSGL  AGNI
Sbjct: 35  MFHSLFVIVLLFSLSNAAVQDFCVADLKGPQGPAGYACKSA--VTADDFVYSGLRSAGNI 94

Query: 61  SSLIKAAVTPAFVEQYPGVNGLGLSAARLDLAPGGVIPLHTHPGASELLLVTQGVIEAGF 120
           +S+IKAAVTPAFVEQYPGVNG+GLSAARLDL PGGVIPLHTHPGASELLLVTQG+I+AGF
Sbjct: 95  TSIIKAAVTPAFVEQYPGVNGIGLSAARLDLLPGGVIPLHTHPGASELLLVTQGIIQAGF 154

Query: 121 ISSANAVYVKTLKKGDLMIFPQGLLHFQINAGGVTAVGFPIFSSPSPGLQITDFALFAND 180
           ISSANAV+VKTLKKGDLMIFPQGLLHFQINAGGVTAVG+PIFSS SPGLQITD+ALFAND
Sbjct: 155 ISSANAVFVKTLKKGDLMIFPQGLLHFQINAGGVTAVGYPIFSSSSPGLQITDYALFAND 214

Query: 181 LSTDLVSKTTFLDPALVKKFKAVLGGSG 209
           LSTDLVSKTTFLDP LVKKFKA+LGGSG
Sbjct: 215 LSTDLVSKTTFLDPVLVKKFKAILGGSG 240

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BLAST of Lag0003136 vs. ExPASy Swiss-Prot
Match: Q9ZRA4 (Auxin-binding protein ABP19a OS=Prunus persica OX=3760 GN=ABP19A PE=3 SV=1)

HSP 1 Score: 292.0 bits (746), Expect = 5.5e-78
Identity = 143/207 (69.08%), Postives = 166/207 (80.19%), Query Frame = 0


Query: 1   MFHSLFIISLLFSLSNAAVQDFCVADLKGPQGPAGYSCKSAADVTADDFVYSGLIGAGNI 60
           +F   F   LL S S+A+VQDFCVAD K P GPAGYSCK  A VT +DFVYSGL  AGN 
Sbjct: 2   IFPIFFTFFLLLSSSHASVQDFCVADYKAPDGPAGYSCKKPAKVTINDFVYSGLGIAGNT 61

Query: 61  SSLIKAAVTPAFVEQYPGVNGLGLSAARLDLAPGGVIPLHTHPGASELLLVTQGVIEAGF 120
           +++IKAAVTPAF  Q+PGVNGLG+S ARLDL PGGVIP HTHPGASE+LLV QG I AGF
Sbjct: 62  TNIIKAAVTPAFAAQFPGVNGLGISLARLDLGPGGVIPFHTHPGASEVLLVVQGTIIAGF 121

Query: 121 ISSANAVYVKTLKKGDLMIFPQGLLHFQINAGGVTAVGFPIFSSPSPGLQITDFALFAND 180
           ++S N  Y+KTLKKGD+M+FPQGLLHFQ+N GG  A+ FP FSSPSPGLQI DFALF ND
Sbjct: 122 VASDNTPYLKTLKKGDIMVFPQGLLHFQVNGGGTPALAFPSFSSPSPGLQILDFALFKND 181

Query: 181 LSTDLVSKTTFLDPALVKKFKAVLGGS 208
           L T+L+++TTFLD A +KK K VLGG+
Sbjct: 182 LPTELIAQTTFLDAAQIKKLKGVLGGT 208

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BLAST of Lag0003136 vs. ExPASy Swiss-Prot
Match: O04012 (Auxin-binding protein ABP19b OS=Prunus persica OX=3760 GN=ABP19B PE=3 SV=1)

HSP 1 Score: 283.5 bits (724), Expect = 1.9e-75
Identity = 137/207 (66.18%), Postives = 163/207 (78.74%), Query Frame = 0


Query: 1   MFHSLFIISLLFSLSNAAVQDFCVADLKGPQGPAGYSCKSAADVTADDFVYSGLIGAGNI 60
           +F   F   LL S S+A+VQDFCVAD K P GPAGYSCK  A VT +DFVYSGL  AGN 
Sbjct: 2   IFPIFFTFFLLLSTSHASVQDFCVADYKAPDGPAGYSCKKPAIVTVNDFVYSGLGIAGNT 61

Query: 61  SSLIKAAVTPAFVEQYPGVNGLGLSAARLDLAPGGVIPLHTHPGASELLLVTQGVIEAGF 120
           +++ KAAVTPAF  Q+PGVNGLG+S ARLDL PGGV+P HTHPGASE+LLV QG I AGF
Sbjct: 62  TNIFKAAVTPAFAAQFPGVNGLGISLARLDLGPGGVVPFHTHPGASEVLLVVQGTIIAGF 121

Query: 121 ISSANAVYVKTLKKGDLMIFPQGLLHFQINAGGVTAVGFPIFSSPSPGLQITDFALFAND 180
           ++S N  Y+KTLKKGD+++FPQGLLHFQ+N G   A+ FP FSSPSPGLQI DFALF ND
Sbjct: 122 VASDNTPYLKTLKKGDIIVFPQGLLHFQVNGGDTPAIAFPSFSSPSPGLQIVDFALFKND 181

Query: 181 LSTDLVSKTTFLDPALVKKFKAVLGGS 208
           L+T+L+++TT LD   +KK K VLGG+
Sbjct: 182 LATELIAQTTLLDAPQIKKLKGVLGGT 208

[Close]

BLAST of Lag0003136 vs. ExPASy Swiss-Prot
Match: O04011 (Auxin-binding protein ABP20 OS=Prunus persica OX=3760 GN=ABP20 PE=2 SV=1)

HSP 1 Score: 276.6 bits (706), Expect = 2.4e-73
Identity = 136/207 (65.70%), Postives = 164/207 (79.23%), Query Frame = 0


Query: 1   MFHSLFIISLLFSLSNAAVQDFCVADLKGPQGPAGYSCKSAADVTADDFVYSGLIGAGNI 60
           +F  LF   LL S SNAAVQDFCVADL  P+GPAG+SCK  A V  +DFV+SGL  AGN 
Sbjct: 7   IFPILFTFFLLLSSSNAAVQDFCVADLAAPEGPAGFSCKKPASVKVNDFVFSGLGIAGNT 66

Query: 61  SSLIKAAVTPAFVEQYPGVNGLGLSAARLDLAPGGVIPLHTHPGASELLLVTQGVIEAGF 120
           S++IKAAVTPAFV Q+PGVNGLG+S ARLDLA GGV+P HTHPGASE+L+V QG I AGF
Sbjct: 67  SNIIKAAVTPAFVAQFPGVNGLGISIARLDLAVGGVVPFHTHPGASEVLIVAQGTICAGF 126

Query: 121 ISSANAVYVKTLKKGDLMIFPQGLLHFQINAGGVTAVGFPIFSSPSPGLQITDFALFAND 180
           ++S N  Y++TL+KGD+M+FPQGLLHFQ+N G   A+ F  F S SPGLQI DFALF ND
Sbjct: 127 VASDNTPYLQTLEKGDIMVFPQGLLHFQVNGGEAPALAFASFGSASPGLQILDFALFKND 186

Query: 181 LSTDLVSKTTFLDPALVKKFKAVLGGS 208
           L T+++++TTFLD A +KK K VLGG+
Sbjct: 187 LPTEVIAQTTFLDAAQIKKLKGVLGGT 213

[Close]

BLAST of Lag0003136 vs. ExPASy Swiss-Prot
Match: L8BRS3 (Germin-like protein OS=Morus alba OX=3498 PE=1 SV=1)

HSP 1 Score: 276.6 bits (706), Expect = 2.4e-73
Identity = 135/203 (66.50%), Postives = 164/203 (80.79%), Query Frame = 0


Query: 6   FIISLLFSLSNAAVQDFCVADLKGPQGPAGYSCKSAADVTADDFVYSGLIGAGNISSLIK 65
           F+ SLLFS S+ A+QDFCVAD   PQGPAGYSCK+ A VT D+FVYSGL   GN ++LIK
Sbjct: 7   FLFSLLFSSSHGAIQDFCVADYSAPQGPAGYSCKNPAKVTVDNFVYSGLGITGNTTNLIK 66

Query: 66  AAVTPAFVEQYPGVNGLGLSAARLDLAPGGVIPLHTHPGASELLLVTQGVIEAGFISSAN 125
           AAVT AF  Q+PGVNGLG+S AR DLAPGGVIP HTHPGASE+++V +G + A F+SS N
Sbjct: 67  AAVTTAFDNQFPGVNGLGISLARPDLAPGGVIPFHTHPGASEIIIVIEGSLCAAFVSSDN 126

Query: 126 AVYVKTLKKGDLMIFPQGLLHFQINAGGVTAVGFPIFSSPSPGLQITDFALFANDLSTDL 185
            VY+K+LKKGD MIFP GLLHFQ+NAG   A+ F  F+SP+PGLQ+ D+ALF NDL+T+L
Sbjct: 127 KVYLKSLKKGDTMIFPSGLLHFQLNAGKNNALFFVAFNSPNPGLQLVDYALFGNDLATEL 186

Query: 186 VSKTTFLDPALVKKFKAVLGGSG 209
           V+  +FLDPA +K+ KAVLGGSG
Sbjct: 187 VAAASFLDPAEIKRLKAVLGGSG 209

[Close]

BLAST of Lag0003136 vs. ExPASy Swiss-Prot
Match: P94072 (Germin-like protein subfamily 3 member 3 OS=Arabidopsis thaliana OX=3702 GN=GER3 PE=1 SV=1)

HSP 1 Score: 267.7 bits (683), Expect = 1.1e-70
Identity = 136/202 (67.33%), Postives = 160/202 (79.21%), Query Frame = 0


Query: 6   FIISLLFSLSNAAVQDFCVADLKGPQGPAGYSCKSAADVTADDFVYSGLIGAGNISSLIK 65
           FIISL+ ++S A+VQDFCVAD KGPQ P+GYSCK+   VT +DF ++GL  AGN S++IK
Sbjct: 9   FIISLISTISFASVQDFCVADPKGPQSPSGYSCKNPDQVTENDFAFTGLGTAGNTSNIIK 68

Query: 66  AAVTPAFVEQYPGVNGLGLSAARLDLAPGGVIPLHTHPGASELLLVTQGVIEAGFISSAN 125
           AAVTPAF   Y G+NGLG+S ARLDLA GGVIPLHTHPGASE+L+V QG I AGFISSAN
Sbjct: 69  AAVTPAFAPAYAGINGLGVSLARLDLAGGGVIPLHTHPGASEVLVVIQGTICAGFISSAN 128

Query: 126 AVYVKTLKKGDLMIFPQGLLHFQINAGGVTAVGFPIFSSPSPGLQITDFALFANDLSTDL 185
            VY+KTL +GD M+FPQGLLHFQ+N+G   A+ F  F S SPGLQI  FALFANDL ++L
Sbjct: 129 KVYLKTLNRGDSMVFPQGLLHFQLNSGKGPALAFVAFGSSSPGLQILPFALFANDLPSEL 188

Query: 186 VSKTTFLDPALVKKFKAVLGGS 208
           V  TTFL  A VKK K VLGG+
Sbjct: 189 VEATTFLSDAEVKKLKGVLGGT 210

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BLAST of Lag0003136 vs. ExPASy TrEMBL
Match: A0A6J1HMR8 (Germin-like protein OS=Cucurbita moschata OX=3662 GN=LOC111464356 PE=3 SV=1)

HSP 1 Score: 384.4 bits (986), Expect = 3.0e-103
Identity = 193/208 (92.79%), Postives = 204/208 (98.08%), Query Frame = 0


Query: 1   MFHSLFIISLLFSLSNAAVQDFCVADLKGPQGPAGYSCKSAADVTADDFVYSGLIGAGNI 60
           MFHSLF+I+LLFSLSNAAVQDFCVADLKGPQGPAGY+CKSA  VTADDFVYSGL GAGNI
Sbjct: 1   MFHSLFVIALLFSLSNAAVQDFCVADLKGPQGPAGYACKSA--VTADDFVYSGLSGAGNI 60

Query: 61  SSLIKAAVTPAFVEQYPGVNGLGLSAARLDLAPGGVIPLHTHPGASELLLVTQGVIEAGF 120
           +SLIKAAVTPAFVEQYPGVNGLGLSAARLDL PGGVIPLHTHPGASELL+VTQG+I+AGF
Sbjct: 61  TSLIKAAVTPAFVEQYPGVNGLGLSAARLDLLPGGVIPLHTHPGASELLIVTQGIIQAGF 120

Query: 121 ISSANAVYVKTLKKGDLMIFPQGLLHFQINAGGVTAVGFPIFSSPSPGLQITDFALFAND 180
           ISSANAV+VKTLKKGDLMIFPQGLLHFQINAGGVTAVG+P+FSSPSPGLQITDFALFAND
Sbjct: 121 ISSANAVFVKTLKKGDLMIFPQGLLHFQINAGGVTAVGYPVFSSPSPGLQITDFALFAND 180

Query: 181 LSTDLVSKTTFLDPALVKKFKAVLGGSG 209
           LSTDLVSKTTFLDPALVKKFKA+LGGSG
Sbjct: 181 LSTDLVSKTTFLDPALVKKFKAILGGSG 206

[Close]

BLAST of Lag0003136 vs. ExPASy TrEMBL
Match: A0A5D3BXR0 (Germin-like protein OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold347G001220 PE=3 SV=1)

HSP 1 Score: 384.0 bits (985), Expect = 3.9e-103
Identity = 194/208 (93.27%), Postives = 200/208 (96.15%), Query Frame = 0


Query: 1   MFHSLFIISLLFSLSNAAVQDFCVADLKGPQGPAGYSCKSAADVTADDFVYSGLIGAGNI 60
           MFHSL IISLLFSLSNAAVQDFCVADLKGPQGPAGYSCK A+ VTADDFVY+GL  AGNI
Sbjct: 1   MFHSLLIISLLFSLSNAAVQDFCVADLKGPQGPAGYSCKIASVVTADDFVYTGLRAAGNI 60

Query: 61  SSLIKAAVTPAFVEQYPGVNGLGLSAARLDLAPGGVIPLHTHPGASELLLVTQGVIEAGF 120
           SSLIKAAVTPAFVEQYPGVNGLGLSAARLDLAPGGVIPLHTHPGASELLLVTQG+IEAGF
Sbjct: 61  SSLIKAAVTPAFVEQYPGVNGLGLSAARLDLAPGGVIPLHTHPGASELLLVTQGIIEAGF 120

Query: 121 ISSANAVYVKTLKKGDLMIFPQGLLHFQINAGGVTAVGFPIFSSPSPGLQITDFALFAND 180
           ISSANAVYVKTLKKGDLM+FPQGLLHFQINAGGVTAVGFP+FSSPSPGLQI DFALFAND
Sbjct: 121 ISSANAVYVKTLKKGDLMVFPQGLLHFQINAGGVTAVGFPVFSSPSPGLQILDFALFAND 180

Query: 181 LSTDLVSKTTFLDPALVKKFKAVLGGSG 209
           LSTDLVSKTTFLDP L+KK KAVLGGSG
Sbjct: 181 LSTDLVSKTTFLDPVLIKKLKAVLGGSG 208

[Close]

BLAST of Lag0003136 vs. ExPASy TrEMBL
Match: A0A0A0KAW6 (Germin-like protein OS=Cucumis sativus OX=3659 GN=Csa_7G450510 PE=3 SV=1)

HSP 1 Score: 381.7 bits (979), Expect = 1.9e-102
Identity = 193/208 (92.79%), Postives = 200/208 (96.15%), Query Frame = 0


Query: 1   MFHSLFIISLLFSLSNAAVQDFCVADLKGPQGPAGYSCKSAADVTADDFVYSGLIGAGNI 60
           M HSL IISLLFSLSNAAVQDFCVADLKGPQGPAGYSCK A+ VTADDFV+SGL  AGNI
Sbjct: 1   MLHSLLIISLLFSLSNAAVQDFCVADLKGPQGPAGYSCKIASVVTADDFVFSGLRAAGNI 60

Query: 61  SSLIKAAVTPAFVEQYPGVNGLGLSAARLDLAPGGVIPLHTHPGASELLLVTQGVIEAGF 120
           SSLIKAAVTPAFVEQYPGVNGLGLSAARLDLAPGGVIPLHTHPGASELLLVTQG+IEAGF
Sbjct: 61  SSLIKAAVTPAFVEQYPGVNGLGLSAARLDLAPGGVIPLHTHPGASELLLVTQGIIEAGF 120

Query: 121 ISSANAVYVKTLKKGDLMIFPQGLLHFQINAGGVTAVGFPIFSSPSPGLQITDFALFAND 180
           ISSANAVYVKTLKKGDLM+FPQGLLHFQINAGGVTAVGFP+FSSPSPGLQI DFALFAND
Sbjct: 121 ISSANAVYVKTLKKGDLMVFPQGLLHFQINAGGVTAVGFPVFSSPSPGLQILDFALFAND 180

Query: 181 LSTDLVSKTTFLDPALVKKFKAVLGGSG 209
           LSTDLVSKTTFLDPAL+KK K+VLGGSG
Sbjct: 181 LSTDLVSKTTFLDPALIKKLKSVLGGSG 208

[Close]

BLAST of Lag0003136 vs. ExPASy TrEMBL
Match: A0A6J1KCE7 (Germin-like protein OS=Cucurbita maxima OX=3661 GN=LOC111494341 PE=3 SV=1)

HSP 1 Score: 374.4 bits (960), Expect = 3.1e-100
Identity = 189/208 (90.87%), Postives = 200/208 (96.15%), Query Frame = 0


Query: 1   MFHSLFIISLLFSLSNAAVQDFCVADLKGPQGPAGYSCKSAADVTADDFVYSGLIGAGNI 60
           MFHSLF+I LLFSLSNAAVQDFCVADLKGPQGPAGY+CKSA  VTADDFVYSGL  AGNI
Sbjct: 35  MFHSLFVIVLLFSLSNAAVQDFCVADLKGPQGPAGYACKSA--VTADDFVYSGLRSAGNI 94

Query: 61  SSLIKAAVTPAFVEQYPGVNGLGLSAARLDLAPGGVIPLHTHPGASELLLVTQGVIEAGF 120
           +S+IKAAVTPAFVEQYPGVNG+GLSAARLDL PGGVIPLHTHPGASELLLVTQG+I+AGF
Sbjct: 95  TSIIKAAVTPAFVEQYPGVNGIGLSAARLDLLPGGVIPLHTHPGASELLLVTQGIIQAGF 154

Query: 121 ISSANAVYVKTLKKGDLMIFPQGLLHFQINAGGVTAVGFPIFSSPSPGLQITDFALFAND 180
           ISSANAV+VKTLKKGDLMIFPQGLLHFQINAGGVTAVG+PIFSS SPGLQITD+ALFAND
Sbjct: 155 ISSANAVFVKTLKKGDLMIFPQGLLHFQINAGGVTAVGYPIFSSSSPGLQITDYALFAND 214

Query: 181 LSTDLVSKTTFLDPALVKKFKAVLGGSG 209
           LSTDLVSKTTFLDP LVKKFKA+LGGSG
Sbjct: 215 LSTDLVSKTTFLDPVLVKKFKAILGGSG 240

[Close]

BLAST of Lag0003136 vs. ExPASy TrEMBL
Match: A0A6J1D2R7 (Germin-like protein OS=Momordica charantia OX=3673 GN=LOC111017042 PE=3 SV=1)

HSP 1 Score: 372.5 bits (955), Expect = 1.2e-99
Identity = 187/208 (89.90%), Postives = 195/208 (93.75%), Query Frame = 0


Query: 1   MFHSLFIISLLFSLSNAAVQDFCVADLKGPQGPAGYSCKSAADVTADDFVYSGLIGAGNI 60
           M H+LFIISLLFSLSNAAVQDFCV DLKGPQGPAGYSCKSA  V  DDFV+SGL  AGNI
Sbjct: 1   MLHALFIISLLFSLSNAAVQDFCVGDLKGPQGPAGYSCKSADKVIVDDFVFSGLRAAGNI 60

Query: 61  SSLIKAAVTPAFVEQYPGVNGLGLSAARLDLAPGGVIPLHTHPGASELLLVTQGVIEAGF 120
           SSLIKAAVTPAFVEQYPGVNGLGLSAARLDLAPGGV+PLHTHPGASELL+VTQG++EAGF
Sbjct: 61  SSLIKAAVTPAFVEQYPGVNGLGLSAARLDLAPGGVVPLHTHPGASELLIVTQGIVEAGF 120

Query: 121 ISSANAVYVKTLKKGDLMIFPQGLLHFQINAGGVTAVGFPIFSSPSPGLQITDFALFAND 180
           ISSANAVYVKTLKKGDLM+FPQGLLHFQINAGGVTAV FP+FSSPSPGLQITDFALFAND
Sbjct: 121 ISSANAVYVKTLKKGDLMVFPQGLLHFQINAGGVTAVAFPVFSSPSPGLQITDFALFAND 180

Query: 181 LSTDLVSKTTFLDPALVKKFKAVLGGSG 209
           LSTDLV KTTFLDP  VKK KAVLGGSG
Sbjct: 181 LSTDLVVKTTFLDPDQVKKLKAVLGGSG 208

[Close]

BLAST of Lag0003136 vs. TAIR 10
Match: AT5G20630.1 (germin 3 )

HSP 1 Score: 267.7 bits (683), Expect = 7.8e-72
Identity = 136/202 (67.33%), Postives = 160/202 (79.21%), Query Frame = 0


Query: 6   FIISLLFSLSNAAVQDFCVADLKGPQGPAGYSCKSAADVTADDFVYSGLIGAGNISSLIK 65
           FIISL+ ++S A+VQDFCVAD KGPQ P+GYSCK+   VT +DF ++GL  AGN S++IK
Sbjct: 9   FIISLISTISFASVQDFCVADPKGPQSPSGYSCKNPDQVTENDFAFTGLGTAGNTSNIIK 68

Query: 66  AAVTPAFVEQYPGVNGLGLSAARLDLAPGGVIPLHTHPGASELLLVTQGVIEAGFISSAN 125
           AAVTPAF   Y G+NGLG+S ARLDLA GGVIPLHTHPGASE+L+V QG I AGFISSAN
Sbjct: 69  AAVTPAFAPAYAGINGLGVSLARLDLAGGGVIPLHTHPGASEVLVVIQGTICAGFISSAN 128

Query: 126 AVYVKTLKKGDLMIFPQGLLHFQINAGGVTAVGFPIFSSPSPGLQITDFALFANDLSTDL 185
            VY+KTL +GD M+FPQGLLHFQ+N+G   A+ F  F S SPGLQI  FALFANDL ++L
Sbjct: 129 KVYLKTLNRGDSMVFPQGLLHFQLNSGKGPALAFVAFGSSSPGLQILPFALFANDLPSEL 188

Query: 186 VSKTTFLDPALVKKFKAVLGGS 208
           V  TTFL  A VKK K VLGG+
Sbjct: 189 VEATTFLSDAEVKKLKGVLGGT 210

[Close]

BLAST of Lag0003136 vs. TAIR 10
Match: AT1G72610.1 (germin-like protein 1 )

HSP 1 Score: 266.9 bits (681), Expect = 1.3e-71
Identity = 136/208 (65.38%), Postives = 161/208 (77.40%), Query Frame = 0


Query: 1   MFHSLFIISLLFSLSNAAVQDFCVADLKGPQGPAGYSCKSAADVTADDFVYSGLIGAGNI 60
           M  ++F++SLLF+LSNA+VQDFCVA+LK  + PAGY C     V A DFV+SGL   GN 
Sbjct: 1   MLRTIFLLSLLFALSNASVQDFCVANLKRAETPAGYPCIRPIHVKATDFVFSGLGTPGNT 60

Query: 61  SSLIKAAVTPAFVEQYPGVNGLGLSAARLDLAPGGVIPLHTHPGASELLLVTQGVIEAGF 120
           +++I AAVTPAF  Q+PG+NGLGLS ARLDLAP GVIP+HTHPGASE+L V  G I AGF
Sbjct: 61  TNIINAAVTPAFAAQFPGLNGLGLSTARLDLAPKGVIPMHTHPGASEVLFVLTGSITAGF 120

Query: 121 ISSANAVYVKTLKKGDLMIFPQGLLHFQINAGGVTAVGFPIFSSPSPGLQITDFALFAND 180
           +SSANAVYV+TLK G +M+FPQGLLHFQINAG  +A     F+S +PGLQI DFALFAN 
Sbjct: 121 VSSANAVYVQTLKPGQVMVFPQGLLHFQINAGKSSASAVVTFNSANPGLQILDFALFANS 180

Query: 181 LSTDLVSKTTFLDPALVKKFKAVLGGSG 209
           L T+LV  TTFLD   VKK K VLGG+G
Sbjct: 181 LPTELVVGTTFLDATTVKKLKGVLGGTG 208

[Close]

BLAST of Lag0003136 vs. TAIR 10
Match: AT1G02335.1 (germin-like protein subfamily 2 member 2 precursor )

HSP 1 Score: 143.3 bits (360), Expect = 2.2e-34
Identity = 79/200 (39.50%), Postives = 120/200 (60.00%), Query Frame = 0


Query: 4   SLFIISLLFSLSNAAVQDFCVADLKGPQGPAGYSCKSAADVTADDFVYSGLIGAGNISSL 63
           S  +I+ + +    A+QD CVAD        G+ CK   ++T  DF ++G+     I+S 
Sbjct: 13  SCIMITSIRAYDPDALQDLCVADKSHGTKLNGFPCKETLNITESDFFFAGISKPAVINST 72

Query: 64  IKAAVTPAFVEQYPGVNGLGLSAARLDLAPGGVIPLHTHPGASELLLVTQGVIEAGFISS 123
           + +AVT A VE+ PG+N L +S AR+D APGG+ P HTHP A+E++ V +G +E GFI++
Sbjct: 73  MGSAVTGANVEKIPGLNTLSVSLARIDYAPGGLNPPHTHPRATEVVYVLEGELEVGFITT 132

Query: 124 ANAVYVKTLKKGDLMIFPQGLLHFQINAGGVTAVGFPIFSSPSPGLQITDFALFAND--L 183
           AN ++ KT+K G++ +FP+GL+HFQ N G   A     F+S  PG       LFA +  L
Sbjct: 133 ANKLFTKTIKIGEVFVFPRGLVHFQKNNGKSPASVLSAFNSQLPGTASVAATLFAAEPAL 192

Query: 184 STDLVSKTTFLDPALVKKFK 202
             D+++KT  +   +V K K
Sbjct: 193 PEDVLTKTFQVGSKMVDKIK 212

[Close]

BLAST of Lag0003136 vs. TAIR 10
Match: AT5G26700.1 (RmlC-like cupins superfamily protein )

HSP 1 Score: 141.0 bits (354), Expect = 1.1e-33
Identity = 78/201 (38.81%), Postives = 114/201 (56.72%), Query Frame = 0


Query: 3   HSLFIISLLFSLSNAA--VQDFCVADLKGPQGPAGYSCKSAADVTADDFVYSGLIGAGNI 62
           H + ++++LF    +A  +QD CVADL       GY+CK +  +T +DF + GL      
Sbjct: 7   HLVVVVTMLFVAMASAEMLQDVCVADLSNAVKVNGYTCKDSTQITPEDFYFKGLANIAAT 66

Query: 63  SSLIKAAVTPAFVEQYPGVNGLGLSAARLDLAPGGVIPLHTHPGASELLLVTQGVIEAGF 122
           ++   + VT A VE+ PG+N LGLS +R+D AP G+ P H HP ASE++ V +G +  GF
Sbjct: 67  NTSTGSVVTGANVEKLPGLNTLGLSMSRIDYAPNGLNPPHVHPRASEIIFVLEGQLYVGF 126

Query: 123 ISSANAVYVKTLKKGDLMIFPQGLLHFQINAGGVTAVGFPIFSSPSPGLQITDFALFAND 182
           +++A  +  K L KGD+  FP+GL+HFQ N     A     F S  PG Q    +LF   
Sbjct: 127 VTTAGKLIAKNLNKGDVFTFPKGLIHFQKNIANSPASVLAAFDSQLPGTQSLVASLF-GA 186

Query: 183 LSTDLVSKTTFLDPALVKKFK 202
           L  D+++K+  L    VKK K
Sbjct: 187 LPDDILAKSFQLKHKQVKKIK 206

[Close]

BLAST of Lag0003136 vs. TAIR 10
Match: AT1G09560.1 (germin-like protein 5 )

HSP 1 Score: 140.2 bits (352), Expect = 1.9e-33
Identity = 80/204 (39.22%), Postives = 120/204 (58.82%), Query Frame = 0


Query: 5   LFIISLLFSLSNAA----VQDFCVADLKGPQGPAGYSCKSAADVTADDFVYSGLIGAGNI 64
           L + ++ F +S++A    +QD CVADL       G+ CK AA VT+ DF   GL   G  
Sbjct: 10  LLLTTVSFFISSSADPDMLQDLCVADLPSGIKINGFPCKDAATVTSADFFSQGLAKPGLT 69

Query: 65  SSLIKAAVTPAFVEQYPGVNGLGLSAARLDLAPGGVIPLHTHPGASELLLVTQGVIEAGF 124
           ++   A VT A V   PG+N LG+S +R+D APGG+ P HTHP A+E++ V +G ++ GF
Sbjct: 70  NNTFGALVTGANVMTIPGLNTLGVSLSRIDYAPGGLNPPHTHPRATEVVFVLEGTLDVGF 129

Query: 125 ISSANAVYVKTLKKGDLMIFPQGLLHFQINAGGVTAVGFPIFSSPSPGLQITDFALFAN- 184
           +++AN +  ++LKKGD+  FP+GL+HFQ N G V A     F+S  PG Q     LF + 
Sbjct: 130 LTTANKLISQSLKKGDVFAFPKGLVHFQKNNGDVPASVIAAFNSQLPGTQSLGATLFGST 189

Query: 185 -DLSTDLVSKTTFLDPALVKKFKA 203
             +  +++++     P  VK  K+
Sbjct: 190 PPVPDNILAQAFQTSPGTVKHIKS 213

The following BLAST results are available for this feature:

BLAST of Lag0003136 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_038897216.1	7.3e-104	94.71	auxin-binding protein ABP19a [Benincasa hispida]	[more]
XP_022964299.1	6.2e-103	92.79	auxin-binding protein ABP19a-like [Cucurbita moschata] >XP_023514157.1 auxin-bin...	[more]
KAA0059402.1	8.0e-103	93.27	auxin-binding protein ABP19a [Cucumis melo var. makuwa] >TYK03925.1 auxin-bindin...	[more]
XP_004141699.1	4.0e-102	92.79	auxin-binding protein ABP19a [Cucumis sativus] >KGN45492.1 hypothetical protein ...	[more]
XP_023000032.1	6.4e-100	90.87	auxin-binding protein ABP19a-like [Cucurbita maxima]	[more]

BLAST of Lag0003136 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
Q9ZRA4	5.5e-78	69.08	Auxin-binding protein ABP19a OS=Prunus persica OX=3760 GN=ABP19A PE=3 SV=1	[more]
O04012	1.9e-75	66.18	Auxin-binding protein ABP19b OS=Prunus persica OX=3760 GN=ABP19B PE=3 SV=1	[more]
O04011	2.4e-73	65.70	Auxin-binding protein ABP20 OS=Prunus persica OX=3760 GN=ABP20 PE=2 SV=1	[more]
L8BRS3	2.4e-73	66.50	Germin-like protein OS=Morus alba OX=3498 PE=1 SV=1	[more]
P94072	1.1e-70	67.33	Germin-like protein subfamily 3 member 3 OS=Arabidopsis thaliana OX=3702 GN=GER3...	[more]

BLAST of Lag0003136 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A6J1HMR8	3.0e-103	92.79	Germin-like protein OS=Cucurbita moschata OX=3662 GN=LOC111464356 PE=3 SV=1	[more]
A0A5D3BXR0	3.9e-103	93.27	Germin-like protein OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold347G...	[more]
A0A0A0KAW6	1.9e-102	92.79	Germin-like protein OS=Cucumis sativus OX=3659 GN=Csa_7G450510 PE=3 SV=1	[more]
A0A6J1KCE7	3.1e-100	90.87	Germin-like protein OS=Cucurbita maxima OX=3661 GN=LOC111494341 PE=3 SV=1	[more]
A0A6J1D2R7	1.2e-99	89.90	Germin-like protein OS=Momordica charantia OX=3673 GN=LOC111017042 PE=3 SV=1	[more]

BLAST of Lag0003136 vs. TAIR 10


Match Name	E-value	Identity	Description
AT5G20630.1	7.8e-72	67.33	germin 3	[more]
AT1G72610.1	1.3e-71	65.38	germin-like protein 1	[more]
AT1G02335.1	2.2e-34	39.50	germin-like protein subfamily 2 member 2 precursor	[more]
AT5G26700.1	1.1e-33	38.81	RmlC-like cupins superfamily protein	[more]
AT1G09560.1	1.9e-33	39.22	germin-like protein 5	[more]

InterPro

Analysis Name: InterPro Annotations of Sponge gourd (AG-4) v1
Date Performed: 2022-08-01


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR001929	Germin	PRINTS	PR00325	GERMIN	coord: 100..120 score: 54.04 coord: 130..150 score: 56.7 coord: 163..178 score: 45.2
IPR006045	Cupin 1	SMART	SM00835	Cupin_1_3	coord: 52..198 e-value: 9.8E-37 score: 138.0
IPR006045	Cupin 1	PFAM	PF00190	Cupin_1	coord: 65..195 e-value: 2.3E-30 score: 105.1
IPR014710	RmlC-like jelly roll fold	GENE3D	2.60.120.10	Jelly Rolls	coord: 16..207 e-value: 3.5E-61 score: 208.1
None	No IPR available	PANTHER	PTHR31238	GERMIN-LIKE PROTEIN SUBFAMILY 3 MEMBER 3	coord: 5..204
None	No IPR available	PANTHER	PTHR31238:SF161	GERMIN-LIKE PROTEIN SUBFAMILY 3 MEMBER 1	coord: 5..204
None	No IPR available	CDD	cd02241	cupin_OxOx	coord: 15..204 e-value: 7.18187E-79 score: 232.486
IPR019780	Germin, manganese binding site	PROSITE	PS00725	GERMIN	coord: 95..108
IPR011051	RmlC-like cupin domain superfamily	SUPERFAMILY	51182	RmlC-like cupins	coord: 17..201

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
Lag0003136.1	Lag0003136.1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
cellular_component	GO:0048046	apoplast
molecular_function	GO:0030145	manganese ion binding