Lag0003130 (gene) Sponge gourd (AG‐4) v1

Overview


Name	Lag0003130
Type	gene
Organism	Luffa acutangula (Sponge gourd (AG‐4) v1)
Description	vacuolar protein sorting-associated protein 2 homolog 1-like
Location	chr4: 48346816 .. 48347297 (+)
RNA-Seq Expression	Lag0003130
Synteny	Lag0003130

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonCDSpolypeptide

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGGGAGAAGCCATGAAAGGTGTTACTAAGGCAATGGGCCAGATGAATAGGCAGATGAACTTGCCGGCATTACAGAAGATAATGCAAGAGTTTGAGAGGCAGAATGAGAGGATGGAAATGACATCTGAAGTAATGGCAGACGCTCTCGATGATGCTTTAGAAGGAGATGAAGAAGAGGAGGAGACAGATGAGCTTGTGAATCAGGTGCTTGATGAGATTGGAATTGATATCAACTCAGAGGTAAACGTGCTTGCATGCATACATTTTTTTCATAATGGTCCATATATTGTCGTGGTCGTGGGAAAAGGTAATTTAAACATAATGGTGATTGCTTTCTGCTATCTCACATGCAGCTTGTGAATGCACCCTCAGCTTCTGTGTCAGTGTCAACTGCAAATAATAGAGTTGCCCAAGCAGAAGCCGAAGATACCGGAATTGACAGTGATTTACAGGCTAGGCTTGATAACTTGAGAAAGATGTAG

mRNA sequence

ATGGGAGAAGCCATGAAAGGTGTTACTAAGGCAATGGGCCAGATGAATAGGCAGATGAACTTGCCGGCATTACAGAAGATAATGCAAGAGTTTGAGAGGCAGAATGAGAGGATGGAAATGACATCTGAAGTAATGGCAGACGCTCTCGATGATGCTTTAGAAGGAGATGAAGAAGAGGAGGAGACAGATGAGCTTGTGAATCAGGTGCTTGATGAGATTGGAATTGATATCAACTCAGAGCTTGTGAATGCACCCTCAGCTTCTGTGTCAGTGTCAACTGCAAATAATAGAGTTGCCCAAGCAGAAGCCGAAGATACCGGAATTGACAGTGATTTACAGGCTAGGCTTGATAACTTGAGAAAGATGTAG

Coding sequence (CDS)

ATGGGAGAAGCCATGAAAGGTGTTACTAAGGCAATGGGCCAGATGAATAGGCAGATGAACTTGCCGGCATTACAGAAGATAATGCAAGAGTTTGAGAGGCAGAATGAGAGGATGGAAATGACATCTGAAGTAATGGCAGACGCTCTCGATGATGCTTTAGAAGGAGATGAAGAAGAGGAGGAGACAGATGAGCTTGTGAATCAGGTGCTTGATGAGATTGGAATTGATATCAACTCAGAGCTTGTGAATGCACCCTCAGCTTCTGTGTCAGTGTCAACTGCAAATAATAGAGTTGCCCAAGCAGAAGCCGAAGATACCGGAATTGACAGTGATTTACAGGCTAGGCTTGATAACTTGAGAAAGATGTAG

Protein sequence

MGEAMKGVTKAMGQMNRQMNLPALQKIMQEFERQNERMEMTSEVMADALDDALEGDEEEEETDELVNQVLDEIGIDINSELVNAPSASVSVSTANNRVAQAEAEDTGIDSDLQARLDNLRKM

Homology

[Close]

BLAST of Lag0003130 vs. NCBI nr
Match: KAA0059408.1 (vacuolar protein sorting-associated protein 2-like protein 1-like [Cucumis melo var. makuwa] >TYK03919.1 vacuolar protein sorting-associated protein 2-like protein 1-like [Cucumis melo var. makuwa])

HSP 1 Score: 220.3 bits (560), Expect = 9.0e-54
Identity = 122/122 (100.00%), Postives = 122/122 (100.00%), Query Frame = 0


Query: 1   MGEAMKGVTKAMGQMNRQMNLPALQKIMQEFERQNERMEMTSEVMADALDDALEGDEEEE 60
           MGEAMKGVTKAMGQMNRQMNLPALQKIMQEFERQNERMEMTSEVMADALDDALEGDEEEE
Sbjct: 150 MGEAMKGVTKAMGQMNRQMNLPALQKIMQEFERQNERMEMTSEVMADALDDALEGDEEEE 209

Query: 61  ETDELVNQVLDEIGIDINSELVNAPSASVSVSTANNRVAQAEAEDTGIDSDLQARLDNLR 120
           ETDELVNQVLDEIGIDINSELVNAPSASVSVSTANNRVAQAEAEDTGIDSDLQARLDNLR
Sbjct: 210 ETDELVNQVLDEIGIDINSELVNAPSASVSVSTANNRVAQAEAEDTGIDSDLQARLDNLR 269

Query: 121 KM 123
           KM
Sbjct: 270 KM 271

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BLAST of Lag0003130 vs. NCBI nr
Match: XP_008462317.1 (PREDICTED: vacuolar protein sorting-associated protein 2 homolog 1-like [Cucumis melo] >XP_038897571.1 vacuolar protein sorting-associated protein 2 homolog 1-like [Benincasa hispida])

HSP 1 Score: 220.3 bits (560), Expect = 9.0e-54
Identity = 122/122 (100.00%), Postives = 122/122 (100.00%), Query Frame = 0


Query: 1   MGEAMKGVTKAMGQMNRQMNLPALQKIMQEFERQNERMEMTSEVMADALDDALEGDEEEE 60
           MGEAMKGVTKAMGQMNRQMNLPALQKIMQEFERQNERMEMTSEVMADALDDALEGDEEEE
Sbjct: 99  MGEAMKGVTKAMGQMNRQMNLPALQKIMQEFERQNERMEMTSEVMADALDDALEGDEEEE 158

Query: 61  ETDELVNQVLDEIGIDINSELVNAPSASVSVSTANNRVAQAEAEDTGIDSDLQARLDNLR 120
           ETDELVNQVLDEIGIDINSELVNAPSASVSVSTANNRVAQAEAEDTGIDSDLQARLDNLR
Sbjct: 159 ETDELVNQVLDEIGIDINSELVNAPSASVSVSTANNRVAQAEAEDTGIDSDLQARLDNLR 218

Query: 121 KM 123
           KM
Sbjct: 219 KM 220

[Close]

BLAST of Lag0003130 vs. NCBI nr
Match: XP_022954248.1 (vacuolar protein sorting-associated protein 2 homolog 1-like [Cucurbita moschata] >XP_022992389.1 vacuolar protein sorting-associated protein 2 homolog 1-like [Cucurbita maxima] >KAG7013995.1 Vacuolar protein sorting-associated protein 2-like 1 [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 218.8 bits (556), Expect = 2.6e-53
Identity = 121/122 (99.18%), Postives = 121/122 (99.18%), Query Frame = 0


Query: 1   MGEAMKGVTKAMGQMNRQMNLPALQKIMQEFERQNERMEMTSEVMADALDDALEGDEEEE 60
           MGEAMKGVTKAMGQMNRQMNLPALQKIMQEFERQNERMEMTSEVMAD LDDALEGDEEEE
Sbjct: 99  MGEAMKGVTKAMGQMNRQMNLPALQKIMQEFERQNERMEMTSEVMADVLDDALEGDEEEE 158

Query: 61  ETDELVNQVLDEIGIDINSELVNAPSASVSVSTANNRVAQAEAEDTGIDSDLQARLDNLR 120
           ETDELVNQVLDEIGIDINSELVNAPSASVSVSTANNRVAQAEAEDTGIDSDLQARLDNLR
Sbjct: 159 ETDELVNQVLDEIGIDINSELVNAPSASVSVSTANNRVAQAEAEDTGIDSDLQARLDNLR 218

Query: 121 KM 123
           KM
Sbjct: 219 KM 220

[Close]

BLAST of Lag0003130 vs. NCBI nr
Match: XP_004141696.1 (vacuolar protein sorting-associated protein 2 homolog 1 [Cucumis sativus] >KGN45498.1 hypothetical protein Csa_016370 [Cucumis sativus])

HSP 1 Score: 217.2 bits (552), Expect = 7.6e-53
Identity = 120/122 (98.36%), Postives = 122/122 (100.00%), Query Frame = 0


Query: 1   MGEAMKGVTKAMGQMNRQMNLPALQKIMQEFERQNERMEMTSEVMADALDDALEGDEEEE 60
           MG+AMKGVTKAMGQMNRQMNLPALQKIMQEFERQNERMEMTSEVMADALDDALEGDEEEE
Sbjct: 99  MGDAMKGVTKAMGQMNRQMNLPALQKIMQEFERQNERMEMTSEVMADALDDALEGDEEEE 158

Query: 61  ETDELVNQVLDEIGIDINSELVNAPSASVSVSTANNRVAQAEAEDTGIDSDLQARLDNLR 120
           ETDELV+QVLDEIGIDINSELVNAPSASVSVSTANNRVAQAEAEDTGIDSDLQARLDNLR
Sbjct: 159 ETDELVSQVLDEIGIDINSELVNAPSASVSVSTANNRVAQAEAEDTGIDSDLQARLDNLR 218

Query: 121 KM 123
           KM
Sbjct: 219 KM 220

[Close]

BLAST of Lag0003130 vs. NCBI nr
Match: KAG6575451.1 (Vacuolar protein sorting-associated protein 2-like 1, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 217.2 bits (552), Expect = 7.6e-53
Identity = 120/122 (98.36%), Postives = 121/122 (99.18%), Query Frame = 0


Query: 1   MGEAMKGVTKAMGQMNRQMNLPALQKIMQEFERQNERMEMTSEVMADALDDALEGDEEEE 60
           MGEAMKGVTKAMGQMNRQMNLPALQKIMQEFERQNERMEMTSEVMAD LDDALEGDEEEE
Sbjct: 99  MGEAMKGVTKAMGQMNRQMNLPALQKIMQEFERQNERMEMTSEVMADVLDDALEGDEEEE 158

Query: 61  ETDELVNQVLDEIGIDINSELVNAPSASVSVSTANNRVAQAEAEDTGIDSDLQARLDNLR 120
           ETDELVNQVLDEIGIDINSELVNAPSASVSVSTANNRVAQAEAEDTGIDSDLQARLDNLR
Sbjct: 159 ETDELVNQVLDEIGIDINSELVNAPSASVSVSTANNRVAQAEAEDTGIDSDLQARLDNLR 218

Query: 121 KM 123
           K+
Sbjct: 219 KI 220

[Close]

BLAST of Lag0003130 vs. ExPASy Swiss-Prot
Match: Q9SKI2 (Vacuolar protein sorting-associated protein 2 homolog 1 OS=Arabidopsis thaliana OX=3702 GN=VPS2.1 PE=1 SV=2)

HSP 1 Score: 183.3 bits (464), Expect = 1.6e-45
Identity = 104/126 (82.54%), Postives = 114/126 (90.48%), Query Frame = 0


Query: 1   MGEAMKGVTKAMGQMNRQMNLPALQKIMQEFERQNERMEMTSEVMADALDDALEGDEEEE 60
           MGEAMKGVTKAMGQMNRQMNLP+LQKIMQEFERQNE+MEM SEVM DA+DDALEGDEEEE
Sbjct: 100 MGEAMKGVTKAMGQMNRQMNLPSLQKIMQEFERQNEKMEMVSEVMGDAIDDALEGDEEEE 159

Query: 61  ETDELVNQVLDEIGIDINSELVNAPSASVSVSTANNRVAQAE---AEDT-GIDSDLQARL 120
           ET++LV+QVLDEIGIDIN ELVNAPS +V+V  A N+V QAE   AED+ GIDSDLQARL
Sbjct: 160 ETEDLVSQVLDEIGIDINQELVNAPSGAVAVPAAKNKVVQAEATGAEDSGGIDSDLQARL 219

Query: 121 DNLRKM 123
           DNLRKM
Sbjct: 220 DNLRKM 225

[Close]

BLAST of Lag0003130 vs. ExPASy Swiss-Prot
Match: Q6IP52 (Charged multivesicular body protein 2a OS=Xenopus laevis OX=8355 GN=chmp2a PE=2 SV=1)

HSP 1 Score: 114.0 bits (284), Expect = 1.2e-24
Identity = 63/121 (52.07%), Postives = 88/121 (72.73%), Query Frame = 0


Query: 1   MGEAMKGVTKAMGQMNRQMNLPALQKIMQEFERQNERMEMTSEVMADALDDALEGDEEEE 60
           M +AMKGVTKAM  MNRQ+ LP +QKIM EFE+Q+E M+M  E+M DA+DDA+  +++EE
Sbjct: 99  MAQAMKGVTKAMATMNRQLKLPQIQKIMMEFEKQSEIMDMKEEMMNDAIDDAMGDEDDEE 158

Query: 61  ETDELVNQVLDEIGIDINSELVNAPSASVSVSTANNRVAQAEAEDTGIDSDLQARLDNLR 120
           E+D +V+QVLDE+G+ +  EL N PS   S+S A  +  +A A     D+DL+ RL+NLR
Sbjct: 159 ESDAVVSQVLDELGLTLTDELSNLPSTGGSLSVAGAKKGEATAALADADADLEERLNNLR 218

Query: 121 K 122
           +
Sbjct: 219 R 219

[Close]

BLAST of Lag0003130 vs. ExPASy Swiss-Prot
Match: Q7ZW25 (Charged multivesicular body protein 2a OS=Danio rerio OX=7955 GN=chmp2a PE=2 SV=1)

HSP 1 Score: 113.2 bits (282), Expect = 2.0e-24
Identity = 62/121 (51.24%), Postives = 89/121 (73.55%), Query Frame = 0


Query: 1   MGEAMKGVTKAMGQMNRQMNLPALQKIMQEFERQNERMEMTSEVMADALDDALEGDEEEE 60
           M +AMKGVTKAM  MNRQ+ LP +QKIM EFERQ+E M+M  E+M DA+DDA+  +++EE
Sbjct: 99  MAQAMKGVTKAMATMNRQLKLPQIQKIMMEFERQSEIMDMKEEMMNDAIDDAMGDEDDEE 158

Query: 61  ETDELVNQVLDEIGIDINSELVNAPSASVSVSTANNRVAQAEAEDTGIDSDLQARLDNLR 120
           E+D +V+QVLDE+G+ ++ EL N P+   S+S A  + A+ +      D+DL+ RL+NLR
Sbjct: 159 ESDAVVSQVLDELGLTLSDELSNLPATGGSLSVAAGKKAEPQPTLADADADLEERLNNLR 218

Query: 121 K 122
           +
Sbjct: 219 R 219

[Close]

BLAST of Lag0003130 vs. ExPASy Swiss-Prot
Match: O43633 (Charged multivesicular body protein 2a OS=Homo sapiens OX=9606 GN=CHMP2A PE=1 SV=1)

HSP 1 Score: 112.5 bits (280), Expect = 3.5e-24
Identity = 65/123 (52.85%), Postives = 87/123 (70.73%), Query Frame = 0


Query: 1   MGEAMKGVTKAMGQMNRQMNLPALQKIMQEFERQNERMEMTSEVMADALDDALEGDEEEE 60
           M +AMKGVTKAMG MNRQ+ LP +QKIM EFERQ E M+M  E+M DA+DDA+  +E+EE
Sbjct: 99  MAQAMKGVTKAMGTMNRQLKLPQIQKIMMEFERQAEIMDMKEEMMNDAIDDAMGDEEDEE 158

Query: 61  ETDELVNQVLDEIGIDINSELVNAPS--ASVSVSTANNRVAQAEAEDTGIDSDLQARLDN 120
           E+D +V+QVLDE+G+ +  EL N PS   S+SV+    +   A +     D+DL+ RL N
Sbjct: 159 ESDAVVSQVLDELGLSLTDELSNLPSTGGSLSVAAGGKKAEAAASALADADADLEERLKN 218

Query: 121 LRK 122
           LR+
Sbjct: 219 LRR 221

[Close]

BLAST of Lag0003130 vs. ExPASy Swiss-Prot
Match: Q6DFS6 (Charged multivesicular body protein 2a OS=Xenopus tropicalis OX=8364 GN=chmp2a PE=2 SV=1)

HSP 1 Score: 112.5 bits (280), Expect = 3.5e-24
Identity = 62/121 (51.24%), Postives = 87/121 (71.90%), Query Frame = 0


Query: 1   MGEAMKGVTKAMGQMNRQMNLPALQKIMQEFERQNERMEMTSEVMADALDDALEGDEEEE 60
           M +AMKGVTKAM  MNRQ+ LP +QKIM EFE+Q+E M+M  E+M DA+DDA+  +++EE
Sbjct: 99  MAQAMKGVTKAMATMNRQLKLPQIQKIMMEFEKQSEIMDMKEEMMNDAIDDAMGDEDDEE 158

Query: 61  ETDELVNQVLDEIGIDINSELVNAPSASVSVSTANNRVAQAEAEDTGIDSDLQARLDNLR 120
           E+D +V+QVLDE+G+ +  EL N PS   S+S A  +  +  A     D+DL+ RL+NLR
Sbjct: 159 ESDAVVSQVLDELGLTLTDELSNLPSTGGSLSVAGAKKGEPSAALADADADLEERLNNLR 218

Query: 121 K 122
           +
Sbjct: 219 R 219

The following BLAST results are available for this feature:

BLAST of Lag0003130 vs. NCBI nr


Match Name	E-value	Identity	Description
KAA0059408.1	9.0e-54	100.00	vacuolar protein sorting-associated protein 2-like protein 1-like [Cucumis melo ...	[more]
XP_008462317.1	9.0e-54	100.00	PREDICTED: vacuolar protein sorting-associated protein 2 homolog 1-like [Cucumis...	[more]
XP_022954248.1	2.6e-53	99.18	vacuolar protein sorting-associated protein 2 homolog 1-like [Cucurbita moschata...	[more]
XP_004141696.1	7.6e-53	98.36	vacuolar protein sorting-associated protein 2 homolog 1 [Cucumis sativus] >KGN45...	[more]
KAG6575451.1	7.6e-53	98.36	Vacuolar protein sorting-associated protein 2-like 1, partial [Cucurbita argyros...	[more]

BLAST of Lag0003130 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
Q9SKI2	1.6e-45	82.54	Vacuolar protein sorting-associated protein 2 homolog 1 OS=Arabidopsis thaliana ...	[more]
Q6IP52	1.2e-24	52.07	Charged multivesicular body protein 2a OS=Xenopus laevis OX=8355 GN=chmp2a PE=2 ...	[more]
Q7ZW25	2.0e-24	51.24	Charged multivesicular body protein 2a OS=Danio rerio OX=7955 GN=chmp2a PE=2 SV=...	[more]
O43633	3.5e-24	52.85	Charged multivesicular body protein 2a OS=Homo sapiens OX=9606 GN=CHMP2A PE=1 SV...	[more]
Q6DFS6	3.5e-24	51.24	Charged multivesicular body protein 2a OS=Xenopus tropicalis OX=8364 GN=chmp2a P...	[more]

InterPro

Analysis Name: InterPro Annotations of Sponge gourd (AG-4) v1
Date Performed: 2022-08-01


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
None	No IPR available	COILS	Coil	Coil	coord: 21..41
None	No IPR available	GENE3D	6.10.140.1230		coord: 1..84 e-value: 4.3E-27 score: 97.1
None	No IPR available	PANTHER	PTHR10476	CHARGED MULTIVESICULAR BODY PROTEIN	coord: 1..122
None	No IPR available	PANTHER	PTHR10476:SF44	VACUOLAR PROTEIN SORTING-ASSOCIATED PROTEIN 2 HOMOLOG 1-LIKE	coord: 1..122
IPR005024	Snf7 family	PFAM	PF03357	Snf7	coord: 2..88 e-value: 2.2E-21 score: 76.2

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
Lag0003130.1	Lag0003130.1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0007034	vacuolar transport
cellular_component	GO:0005768	endosome