Lag0003129 (gene) Sponge gourd (AG‐4) v1

Overview


Name	Lag0003129
Type	gene
Organism	Luffa acutangula (Sponge gourd (AG‐4) v1)
Description	vacuolar protein sorting-associated protein 2 homolog 1-like
Location	chr4: 48344401 .. 48345575 (+)
RNA-Seq Expression	Lag0003129
Synteny	Lag0003129

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonCDSpolypeptide

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGCTTAGTTCCTTGCCTGAGTGGGCTTTGTATCCAAGCCCATTTAGTTCAACAATTATGGGCTCATGGATTTTCCCATCTTCCTACGGCCCAACTTGTGGTCGATCAGGAAGGCCCAATAATCATTACGGCCGGGCCTTAAGTCCAGAACCTTGCTTTTACGTACCGATTAAGGTGGCTCCTCCATGGCGAGAGCTCACCGTGGCCGGTGGATTCGTCGACGGACTTCGAACAGGGACAGAGTTGCAGAGTAACGGAAGGTGAAGCGAAGAAGGAATTAAGCTTATCGGGATCGGAAATTGCTCGCATCTTTTGGTTTTATCGATCGACGGTCAAGTATGAGCTTCCTCTTTGGCAGCCGAAAAACTCCCGCAGGTTCGTATCCTTCTTACCTTCTCTAATTATGCATATTCTGAAGCTTTTTTCTTGATGATTGCGTGATTTCTTGTGATGAATTTCTAACTCTGAAGTTTAGTTCTATTAGCGTCAATTTGATGTGGATGAAATTATGCGTTCTTACCTTCTCAATTAGTGCATACTCCGGAGCTTTGTTCTTGATGATGGCGTGCTTTCTTGTGATGAATTCTACTCTGGAGTTCAGGCCTGTAAACGTCAATTGGATGTGGATTAAATTACATGAAATTTTACTCGTGATGTCGAGGAATTAAAACTTTCCTATGCGTTATAACGTGTATAAGTAATTTCGCGCGTTCTATTACAATAGACGGCTCGAGATTGTGATCTGTTATGGTTTTCTGTGCAGAGCTTCTGCGGGAGAACAAGAGGATGCTAGATAAGTCCATTAGAGAAATAGAGCGGGAAAGACAAGGCTTACAATCTCAGGAAAAGAAGTTGATTGTCGAGATAAAGAAAAATGCTAAACAAGGACAAATGGTGAGTTAAGCAATTTAGCACTTGTTCTTTGACATCGACACATTTTCAGTGACTGCATTTCTTTAGATTTATTGGTAGTAAGTATGCTTCTACTCTTAACTCGAAATCTAGTCTTCGTTTCTTTCGTTTCTTTAGGAAAAGGCGATGGATACTAGGTTGGACTTGGAACCATCAACTCCGACAATGTTCTTGATTCATTTTGTCTTTTTCAGGGAGCTGTTAGAATAATGGCCAAAGATCTTATCAGGACACGACATCAGATTGAAAAGTTTATAAACTAA

mRNA sequence

ATGCTTAGTTCCTTGCCTGAGTGGGCTTTGTATCCAAGCCCATTTAGTTCAACAATTATGGGCTCATGGATTTTCCCATCTTCCTACGGCCCAACTTGTGGTCGATCAGGAAGGCCCAATAATCATTACGGCCGGGCCTTAAGTCCAGAACCTTGCTTTTACGTACCGATTAAGGTGGCTCCTCCATGGCGAGAGCTCACCGTGGCCGGTGGATTCGTCGACGGACTTCGAACAGGGACAGAGTTGCAGAGTAACGGAAGCCGAAAAACTCCCGCAGAGCTTCTGCGGGAGAACAAGAGGATGCTAGATAAGTCCATTAGAGAAATAGAGCGGGAAAGACAAGGCTTACAATCTCAGGAAAAGAAGTTGATTGTCGAGATAAAGAAAAATGCTAAACAAGGACAAATGGGAGCTGTTAGAATAATGGCCAAAGATCTTATCAGGACACGACATCAGATTGAAAAGTTTATAAACTAA

Coding sequence (CDS)

ATGCTTAGTTCCTTGCCTGAGTGGGCTTTGTATCCAAGCCCATTTAGTTCAACAATTATGGGCTCATGGATTTTCCCATCTTCCTACGGCCCAACTTGTGGTCGATCAGGAAGGCCCAATAATCATTACGGCCGGGCCTTAAGTCCAGAACCTTGCTTTTACGTACCGATTAAGGTGGCTCCTCCATGGCGAGAGCTCACCGTGGCCGGTGGATTCGTCGACGGACTTCGAACAGGGACAGAGTTGCAGAGTAACGGAAGCCGAAAAACTCCCGCAGAGCTTCTGCGGGAGAACAAGAGGATGCTAGATAAGTCCATTAGAGAAATAGAGCGGGAAAGACAAGGCTTACAATCTCAGGAAAAGAAGTTGATTGTCGAGATAAAGAAAAATGCTAAACAAGGACAAATGGGAGCTGTTAGAATAATGGCCAAAGATCTTATCAGGACACGACATCAGATTGAAAAGTTTATAAACTAA

Protein sequence

MLSSLPEWALYPSPFSSTIMGSWIFPSSYGPTCGRSGRPNNHYGRALSPEPCFYVPIKVAPPWRELTVAGGFVDGLRTGTELQSNGSRKTPAELLRENKRMLDKSIREIERERQGLQSQEKKLIVEIKKNAKQGQMGAVRIMAKDLIRTRHQIEKFIN

Homology

[Close]

BLAST of Lag0003129 vs. NCBI nr
Match: XP_004141696.1 (vacuolar protein sorting-associated protein 2 homolog 1 [Cucumis sativus] >KGN45498.1 hypothetical protein Csa_016370 [Cucumis sativus])

HSP 1 Score: 130.6 bits (327), Expect = 1.2e-26
Identity = 70/71 (98.59%), Postives = 71/71 (100.00%), Query Frame = 0


Query: 86  GSRKTPAELLRENKRMLDKSIREIERERQGLQSQEKKLIVEIKKNAKQGQMGAVRIMAKD 145
           GSRKTPAELLRENKRMLDKSIREIERERQGLQSQEKKLIVEIKKNAKQGQMGAV+IMAKD
Sbjct: 6   GSRKTPAELLRENKRMLDKSIREIERERQGLQSQEKKLIVEIKKNAKQGQMGAVKIMAKD 65

Query: 146 LIRTRHQIEKF 157
           LIRTRHQIEKF
Sbjct: 66  LIRTRHQIEKF 76

[Close]

BLAST of Lag0003129 vs. NCBI nr
Match: KAG6575451.1 (Vacuolar protein sorting-associated protein 2-like 1, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 130.6 bits (327), Expect = 1.2e-26
Identity = 70/71 (98.59%), Postives = 71/71 (100.00%), Query Frame = 0


Query: 86  GSRKTPAELLRENKRMLDKSIREIERERQGLQSQEKKLIVEIKKNAKQGQMGAVRIMAKD 145
           GSRKTPAELLRENKRMLDKSIREIERERQGLQSQEKKLIVEIKKNAKQGQMGAV+IMAKD
Sbjct: 6   GSRKTPAELLRENKRMLDKSIREIERERQGLQSQEKKLIVEIKKNAKQGQMGAVKIMAKD 65

Query: 146 LIRTRHQIEKF 157
           LIRTRHQIEKF
Sbjct: 66  LIRTRHQIEKF 76

[Close]

BLAST of Lag0003129 vs. NCBI nr
Match: XP_022954248.1 (vacuolar protein sorting-associated protein 2 homolog 1-like [Cucurbita moschata] >XP_022992389.1 vacuolar protein sorting-associated protein 2 homolog 1-like [Cucurbita maxima] >KAG7013995.1 Vacuolar protein sorting-associated protein 2-like 1 [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 130.6 bits (327), Expect = 1.2e-26
Identity = 70/71 (98.59%), Postives = 71/71 (100.00%), Query Frame = 0


Query: 86  GSRKTPAELLRENKRMLDKSIREIERERQGLQSQEKKLIVEIKKNAKQGQMGAVRIMAKD 145
           GSRKTPAELLRENKRMLDKSIREIERERQGLQSQEKKLIVEIKKNAKQGQMGAV+IMAKD
Sbjct: 6   GSRKTPAELLRENKRMLDKSIREIERERQGLQSQEKKLIVEIKKNAKQGQMGAVKIMAKD 65

Query: 146 LIRTRHQIEKF 157
           LIRTRHQIEKF
Sbjct: 66  LIRTRHQIEKF 76

[Close]

BLAST of Lag0003129 vs. NCBI nr
Match: XP_022149334.1 (vacuolar protein sorting-associated protein 2 homolog 1 [Momordica charantia])

HSP 1 Score: 130.6 bits (327), Expect = 1.2e-26
Identity = 70/71 (98.59%), Postives = 71/71 (100.00%), Query Frame = 0


Query: 86  GSRKTPAELLRENKRMLDKSIREIERERQGLQSQEKKLIVEIKKNAKQGQMGAVRIMAKD 145
           GSRKTPAELLRENKRMLDKSIREIERERQGLQSQEKKLIVEIKKNAKQGQMGAV+IMAKD
Sbjct: 6   GSRKTPAELLRENKRMLDKSIREIERERQGLQSQEKKLIVEIKKNAKQGQMGAVKIMAKD 65

Query: 146 LIRTRHQIEKF 157
           LIRTRHQIEKF
Sbjct: 66  LIRTRHQIEKF 76

[Close]

BLAST of Lag0003129 vs. NCBI nr
Match: XP_023547513.1 (vacuolar protein sorting-associated protein 2 homolog 1-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 130.6 bits (327), Expect = 1.2e-26
Identity = 70/71 (98.59%), Postives = 71/71 (100.00%), Query Frame = 0


Query: 86  GSRKTPAELLRENKRMLDKSIREIERERQGLQSQEKKLIVEIKKNAKQGQMGAVRIMAKD 145
           GSRKTPAELLRENKRMLDKSIREIERERQGLQSQEKKLIVEIKKNAKQGQMGAV+IMAKD
Sbjct: 6   GSRKTPAELLRENKRMLDKSIREIERERQGLQSQEKKLIVEIKKNAKQGQMGAVKIMAKD 65

Query: 146 LIRTRHQIEKF 157
           LIRTRHQIEKF
Sbjct: 66  LIRTRHQIEKF 76

[Close]

BLAST of Lag0003129 vs. ExPASy Swiss-Prot
Match: Q9SKI2 (Vacuolar protein sorting-associated protein 2 homolog 1 OS=Arabidopsis thaliana OX=3702 GN=VPS2.1 PE=1 SV=2)

HSP 1 Score: 122.5 bits (306), Expect = 4.3e-27
Identity = 65/71 (91.55%), Postives = 68/71 (95.77%), Query Frame = 0


Query: 86  GSRKTPAELLRENKRMLDKSIREIERERQGLQSQEKKLIVEIKKNAKQGQMGAVRIMAKD 145
           G RKTPAELLRENKRMLDKSIREIERERQGLQ+QEKKLI EIKK AKQGQMGAV++MAKD
Sbjct: 7   GKRKTPAELLRENKRMLDKSIREIERERQGLQTQEKKLINEIKKTAKQGQMGAVKVMAKD 66

Query: 146 LIRTRHQIEKF 157
           LIRTRHQIEKF
Sbjct: 67  LIRTRHQIEKF 77

[Close]

BLAST of Lag0003129 vs. ExPASy Swiss-Prot
Match: Q5ZHN1 (Charged multivesicular body protein 2a OS=Gallus gallus OX=9031 GN=CHMP2A PE=2 SV=1)

HSP 1 Score: 94.0 bits (232), Expect = 1.7e-18
Identity = 47/72 (65.28%), Postives = 63/72 (87.50%), Query Frame = 0


Query: 86  GSRKTPAELLRENKRMLDKSIREIERERQGLQSQEKKLIVEIKKNAKQGQMGAVRIMAKD 145
           G RKTP ELLR+N+R L +++RE++RERQ L++QEKK+I++IKK AKQGQM AV+IMAKD
Sbjct: 6   GRRKTPEELLRQNQRALTRAMRELDRERQKLEAQEKKIIIDIKKMAKQGQMDAVKIMAKD 65

Query: 146 LIRTRHQIEKFI 158
           L+RTR  ++KFI
Sbjct: 66  LVRTRRYVKKFI 77

[Close]

BLAST of Lag0003129 vs. ExPASy Swiss-Prot
Match: O43633 (Charged multivesicular body protein 2a OS=Homo sapiens OX=9606 GN=CHMP2A PE=1 SV=1)

HSP 1 Score: 94.0 bits (232), Expect = 1.7e-18
Identity = 47/72 (65.28%), Postives = 62/72 (86.11%), Query Frame = 0


Query: 86  GSRKTPAELLRENKRMLDKSIREIERERQGLQSQEKKLIVEIKKNAKQGQMGAVRIMAKD 145
           G RKTP ELLR+N+R L++++RE++RERQ L++QEKK+I +IKK AKQGQM AVRIMAKD
Sbjct: 6   GRRKTPEELLRQNQRALNRAMRELDRERQKLETQEKKIIADIKKMAKQGQMDAVRIMAKD 65

Query: 146 LIRTRHQIEKFI 158
           L+RTR  + KF+
Sbjct: 66  LVRTRRYVRKFV 77

[Close]

BLAST of Lag0003129 vs. ExPASy Swiss-Prot
Match: Q9DB34 (Charged multivesicular body protein 2a OS=Mus musculus OX=10090 GN=Chmp2a PE=1 SV=1)

HSP 1 Score: 94.0 bits (232), Expect = 1.7e-18
Identity = 47/72 (65.28%), Postives = 62/72 (86.11%), Query Frame = 0


Query: 86  GSRKTPAELLRENKRMLDKSIREIERERQGLQSQEKKLIVEIKKNAKQGQMGAVRIMAKD 145
           G RKTP ELLR+N+R L++++RE++RERQ L++QEKK+I +IKK AKQGQM AVRIMAKD
Sbjct: 6   GRRKTPEELLRQNQRALNRAMRELDRERQKLETQEKKIIADIKKMAKQGQMDAVRIMAKD 65

Query: 146 LIRTRHQIEKFI 158
           L+RTR  + KF+
Sbjct: 66  LVRTRRYVRKFV 77

[Close]

BLAST of Lag0003129 vs. ExPASy Swiss-Prot
Match: Q6IP52 (Charged multivesicular body protein 2a OS=Xenopus laevis OX=8355 GN=chmp2a PE=2 SV=1)

HSP 1 Score: 93.2 bits (230), Expect = 2.8e-18
Identity = 47/72 (65.28%), Postives = 62/72 (86.11%), Query Frame = 0


Query: 86  GSRKTPAELLRENKRMLDKSIREIERERQGLQSQEKKLIVEIKKNAKQGQMGAVRIMAKD 145
           G RKTP ELLR+N+R L++++RE++RERQ L+ QEKK+I +IKK AKQGQM AV+IMAKD
Sbjct: 6   GRRKTPEELLRQNQRALNRAMRELDRERQKLEQQEKKIIADIKKMAKQGQMDAVKIMAKD 65

Query: 146 LIRTRHQIEKFI 158
           L+RTR  ++KFI
Sbjct: 66  LVRTRRYVKKFI 77

The following BLAST results are available for this feature:

BLAST of Lag0003129 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_004141696.1	1.2e-26	98.59	vacuolar protein sorting-associated protein 2 homolog 1 [Cucumis sativus] >KGN45...	[more]
KAG6575451.1	1.2e-26	98.59	Vacuolar protein sorting-associated protein 2-like 1, partial [Cucurbita argyros...	[more]
XP_022954248.1	1.2e-26	98.59	vacuolar protein sorting-associated protein 2 homolog 1-like [Cucurbita moschata...	[more]
XP_022149334.1	1.2e-26	98.59	vacuolar protein sorting-associated protein 2 homolog 1 [Momordica charantia]	[more]
XP_023547513.1	1.2e-26	98.59	vacuolar protein sorting-associated protein 2 homolog 1-like [Cucurbita pepo sub...	[more]

BLAST of Lag0003129 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
Q9SKI2	4.3e-27	91.55	Vacuolar protein sorting-associated protein 2 homolog 1 OS=Arabidopsis thaliana ...	[more]
Q5ZHN1	1.7e-18	65.28	Charged multivesicular body protein 2a OS=Gallus gallus OX=9031 GN=CHMP2A PE=2 S...	[more]
O43633	1.7e-18	65.28	Charged multivesicular body protein 2a OS=Homo sapiens OX=9606 GN=CHMP2A PE=1 SV...	[more]
Q9DB34	1.7e-18	65.28	Charged multivesicular body protein 2a OS=Mus musculus OX=10090 GN=Chmp2a PE=1 S...	[more]
Q6IP52	2.8e-18	65.28	Charged multivesicular body protein 2a OS=Xenopus laevis OX=8355 GN=chmp2a PE=2 ...	[more]

InterPro

Analysis Name: InterPro Annotations of Sponge gourd (AG-4) v1
Date Performed: 2022-08-01


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
None	No IPR available	COILS	Coil	Coil	coord: 92..126
None	No IPR available	GENE3D	6.10.140.1230		coord: 85..158 e-value: 6.5E-26 score: 93.2
None	No IPR available	PANTHER	PTHR10476	CHARGED MULTIVESICULAR BODY PROTEIN	coord: 86..157
None	No IPR available	PANTHER	PTHR10476:SF44	VACUOLAR PROTEIN SORTING-ASSOCIATED PROTEIN 2 HOMOLOG 1-LIKE	coord: 86..157
IPR005024	Snf7 family	PFAM	PF03357	Snf7	coord: 97..156 e-value: 2.8E-10 score: 40.1

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
Lag0003129.1	Lag0003129.1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0032509	endosome transport via multivesicular body sorting pathway
biological_process	GO:0070676	intralumenal vesicle formation
biological_process	GO:0045324	late endosome to vacuole transport
biological_process	GO:0015031	protein transport
biological_process	GO:0007034	vacuolar transport
cellular_component	GO:0000815	ESCRT III complex
cellular_component	GO:0005771	multivesicular body