Lag0003075 (gene) Sponge gourd (AG‐4) v1

BLAST of Lag0003075 vs. NCBI nr
Match: XP_038898020.1 (LRR receptor-like serine/threonine-protein kinase IOS1 isoform X3 [Benincasa hispida])

HSP 1 Score: 1114.8 bits (2882), Expect = 0.0e+00
Identity = 607/888 (68.36%), Postives = 661/888 (74.44%), Query Frame = 0

Query: 1   MIMEILRS-AFGFLGCLAALQVLVQAQVQSSFISLDCGLPANSSYEESTTGINYISDAAY 60
           MIMEILR   FG LGCLAA+Q+LV AQ Q+ FISLDCGLP NSSYEE TTGINYISDAAY
Sbjct: 1   MIMEILRPLLFGLLGCLAAVQILVLAQDQTGFISLDCGLPENSSYEELTTGINYISDAAY 60

Query: 61  INTVK------------------VKIYPPNTEIYINDRCG-------RFGFLYGNYDGLD 120
           I+T +                  ++ +   T    N           R  FLYGNYDG +
Sbjct: 61  ISTGESQDLTSKYKNIYERQFWNLRYFSTGTRNCYNVSINRGTKYLIRASFLYGNYDGFN 120

Query: 121 RLPIFNLYVGQSVWDSVEFTEVDIDVWKEIIHIPLTDNIYICLINTNQGTPFISALEFRP 180
           RLP FNLYVGQS+W SVEFTEVD+ +WK+IIHIPLTDNIYICLIN NQGTPFISALEFRP
Sbjct: 121 RLPKFNLYVGQSLWGSVEFTEVDLYLWKDIIHIPLTDNIYICLININQGTPFISALEFRP 180

Query: 181 LPNSTYTTTAGSLSLVSRANFGTDRTYRFGYDVFDRIWTPFTYHEWTNISTSNTVDLTNH 240
           LPN+TYTTT GSLSL SRANFGTD+TYRFGYDVFDRIW PFTY +WTN+STSNTVD TNH
Sbjct: 181 LPNTTYTTTGGSLSLYSRANFGTDQTYRFGYDVFDRIWLPFTYKKWTNVSTSNTVDPTNH 240

Query: 241 DIYEVPSIVMSTAATPTNSSSSLEFKWNSDEVTPQYYAYMYSAEIVKLQANQTRVFNVNL 300
            IYEVPSIVMSTAATPTNSSS LEFKWN DEVT QYYAYM  AEIVKLQANQTRVFNVNL
Sbjct: 241 AIYEVPSIVMSTAATPTNSSSPLEFKWNPDEVTSQYYAYMSFAEIVKLQANQTRVFNVNL 300

Query: 301 NGQHLFGPLSPIYLSTRTFYSETALYDPDGKYVVSLASTENSTLPPLINAFEVYKVIYLS 360
           NGQHLFGPLSPIYLST+TFYSETALYD +G+Y +SL STENSTLPPLINAFEVYKVI +S
Sbjct: 301 NGQHLFGPLSPIYLSTKTFYSETALYDANGQYEISLVSTENSTLPPLINAFEVYKVINIS 360

Query: 361 DLQTDPAD---------------------------------------------------- 420
           DL+TD AD                                                    
Sbjct: 361 DLRTDTADVNVIMSIKLTYGVNKDWQADPCLPKAYPWSGLICNNDTAPRIISLDLSSSDL 420

Query: 421 -------------------------GKVPDFLVNMSSLKVIKLDKNNLTSPLPPELLKRV 480
                                    GKVPDFL NM SLKVIKLDKNNLT PLPPELLKRV
Sbjct: 421 TGEISPYIFNLSMLQTLDLSNNSLSGKVPDFLANMPSLKVIKLDKNNLTGPLPPELLKRV 480

Query: 481 NNDSLTLSVEDNPNLDSACASNSCSEEKKKSFVVPVVAPIGGLLVILTVAAVIFWIIISW 540
           NN+SLTLSVEDNPNLD ACAS+SC+E+KKKSF+VPVVA +GGLLV+LTVAA+IFWI+   
Sbjct: 481 NNNSLTLSVEDNPNLDGACASDSCAEKKKKSFIVPVVASVGGLLVVLTVAAIIFWIVKLR 540

Query: 541 NKPHGQTTVPVPRVKDTECGNDSLEVRRRQFTFTEVMRMTDNFARPLGEGGSGKVYLGHI 600
           NK    +TVPVPRVKDT+  NDSLEVRRRQFT++E++RMT+NFARP+GEGGSG+VYLGHI
Sbjct: 541 NKSQDHSTVPVPRVKDTKSNNDSLEVRRRQFTYSEIVRMTENFARPVGEGGSGRVYLGHI 600

Query: 601 DNVQVAVKMLSPLSAHSYQQFQTEVKLLMRVYHGNLTSLVGYLNEKNHFGLIYEYMTKGN 660
           +NVQVAVKMLSPLSAHSYQQFQTEVKLLMRVYHGNLTSLVGYLNEK+HFGLIYEYMTKGN
Sbjct: 601 ENVQVAVKMLSPLSAHSYQQFQTEVKLLMRVYHGNLTSLVGYLNEKDHFGLIYEYMTKGN 660

Query: 661 LPQVLSEDAVLLTRENRLQIAIGAAQGLEYLRHGCKPPIVHRDVKTTNILLTDDFQAKLA 720
           L QVLSE  VLLT E+RL+IAI AAQGLEYL HGCKPPIVHRDVKTTNILLTD+FQAKLA
Sbjct: 661 LAQVLSESPVLLTWEDRLRIAIDAAQGLEYLHHGCKPPIVHRDVKTTNILLTDNFQAKLA 720

Query: 721 DFGLSKSFPKDDSKTHMSTMVAGTPDYFDPEKRSHNSMGDRH------------------ 751
           DFGLSKSFP D SKTHMST VAGTP YFDPE  + N + ++                   
Sbjct: 721 DFGLSKSFPNDGSKTHMSTTVAGTPGYFDPEYYTSNRLTEKSDVYSFGVVLLEIITCRPV 780

BLAST of Lag0003075 vs. NCBI nr
Match: XP_038898018.1 (LRR receptor-like serine/threonine-protein kinase IOS1 isoform X1 [Benincasa hispida])

HSP 1 Score: 1095.5 bits (2832), Expect = 0.0e+00
Identity = 607/927 (65.48%), Postives = 661/927 (71.31%), Query Frame = 0

Query: 1   MIMEILRS-AFGFLGCLAALQVLVQAQVQSSFISLDCGLPANSSYEESTTGINYISDAAY 60
           MIMEILR   FG LGCLAA+Q+LV AQ Q+ FISLDCGLP NSSYEE TTGINYISDAAY
Sbjct: 1   MIMEILRPLLFGLLGCLAAVQILVLAQDQTGFISLDCGLPENSSYEELTTGINYISDAAY 60

Query: 61  INTVK------------------VKIYPPNTEIYINDRCG-------RFGFLYGNYDGLD 120
           I+T +                  ++ +   T    N           R  FLYGNYDG +
Sbjct: 61  ISTGESQDLTSKYKNIYERQFWNLRYFSTGTRNCYNVSINRGTKYLIRASFLYGNYDGFN 120

Query: 121 RLPIFNLYVGQSVWDSVEFTEVDIDVWKEIIHIPLTDNIYICLINTNQGTPFISALEFRP 180
           RLP FNLYVGQS+W SVEFTEVD+ +WK+IIHIPLTDNIYICLIN NQGTPFISALEFRP
Sbjct: 121 RLPKFNLYVGQSLWGSVEFTEVDLYLWKDIIHIPLTDNIYICLININQGTPFISALEFRP 180

Query: 181 LPNSTYTTTAGSLSLVSRANFGTDRTYRFGYDVFDRIWTPFTYHEWTNISTSNTVDLTNH 240
           LPN+TYTTT GSLSL SRANFGTD+TYRFGYDVFDRIW PFTY +WTN+STSNTVD TNH
Sbjct: 181 LPNTTYTTTGGSLSLYSRANFGTDQTYRFGYDVFDRIWLPFTYKKWTNVSTSNTVDPTNH 240

Query: 241 DIYEVPSIVMSTAATPTNSSSSLEFKWNSDEVTPQYYAYMYSAEIVKLQANQTRVFNVNL 300
            IYEVPSIVMSTAATPTNSSS LEFKWN DEVT QYYAYM  AEIVKLQANQTRVFNVNL
Sbjct: 241 AIYEVPSIVMSTAATPTNSSSPLEFKWNPDEVTSQYYAYMSFAEIVKLQANQTRVFNVNL 300

Query: 301 NGQHLFGPLSPIYLSTRTFYSETALYDPDGKYVVSLASTENSTLPPLINAFEVYKVIYLS 360
           NGQHLFGPLSPIYLST+TFYSETALYD +G+Y +SL STENSTLPPLINAFEVYKVI +S
Sbjct: 301 NGQHLFGPLSPIYLSTKTFYSETALYDANGQYEISLVSTENSTLPPLINAFEVYKVINIS 360

Query: 361 DLQTDPAD---------------------------------------------------- 420
           DL+TD AD                                                    
Sbjct: 361 DLRTDTADVNVIMSIKLTYGVNKDWQADPCLPKAYPWSGLICNNDTAPRIISLDLSSSDL 420

Query: 421 -------------------------GKVPDFLVNMSSLKVIKLDKNNLTSPLPPELLKRV 480
                                    GKVPDFL NM SLKVIKLDKNNLT PLPPELLKRV
Sbjct: 421 TGEISPYIFNLSMLQTLDLSNNSLSGKVPDFLANMPSLKVIKLDKNNLTGPLPPELLKRV 480

Query: 481 NNDSLTLSVEDNPNLDSACASNSCSEEKKKSFVVPVVAPIGGLLVILTVAAVIFWIIISW 540
           NN+SLTLSVEDNPNLD ACAS+SC+E+KKKSF+VPVVA +GGLLV+LTVAA+IFWI+   
Sbjct: 481 NNNSLTLSVEDNPNLDGACASDSCAEKKKKSFIVPVVASVGGLLVVLTVAAIIFWIVKLR 540

Query: 541 NKPHGQTTVPVPRVKDTECGNDSLEVRRRQFTFTEVMRMTDNFARPLGEGGSGKVYLGHI 600
           NK    +TVPVPRVKDT+  NDSLEVRRRQFT++E++RMT+NFARP+GEGGSG+VYLGHI
Sbjct: 541 NKSQDHSTVPVPRVKDTKSNNDSLEVRRRQFTYSEIVRMTENFARPVGEGGSGRVYLGHI 600

Query: 601 DNVQVAVKMLSPLSAHSYQQFQTEVKLLMRVYHGNLTSLVGYLNEKNHFGLIYEYMTKGN 660
           +NVQVAVKMLSPLSAHSYQQFQTEVKLLMRVYHGNLTSLVGYLNEK+HFGLIYEYMTKGN
Sbjct: 601 ENVQVAVKMLSPLSAHSYQQFQTEVKLLMRVYHGNLTSLVGYLNEKDHFGLIYEYMTKGN 660

Query: 661 LPQVLSEDAVLLTRENRLQIAIGAAQ---------------------------------- 720
           L QVLSE  VLLT E+RL+IAI AAQ                                  
Sbjct: 661 LAQVLSESPVLLTWEDRLRIAIDAAQGEKTQQNYRVYSVLSTNTLLLTFLLHHKNEILSQ 720

Query: 721 -----GLEYLRHGCKPPIVHRDVKTTNILLTDDFQAKLADFGLSKSFPKDDSKTHMSTMV 751
                GLEYL HGCKPPIVHRDVKTTNILLTD+FQAKLADFGLSKSFP D SKTHMST V
Sbjct: 721 ILKNTGLEYLHHGCKPPIVHRDVKTTNILLTDNFQAKLADFGLSKSFPNDGSKTHMSTTV 780

BLAST of Lag0003075 vs. NCBI nr
Match: XP_038898019.1 (LRR receptor-like serine/threonine-protein kinase IOS1 isoform X2 [Benincasa hispida])

HSP 1 Score: 1045.0 bits (2701), Expect = 3.0e-301
Identity = 588/908 (64.76%), Postives = 640/908 (70.48%), Query Frame = 0

Query: 1   MIMEILRS-AFGFLGCLAALQVLVQAQVQ------SSFISLDCGLPANSSYEESTTGINY 60
           MIMEILR   FG LGCLAA+Q+LV AQ Q      S+  S D      + YE     + Y
Sbjct: 1   MIMEILRPLLFGLLGCLAAVQILVLAQDQTDAAYISTGESQDLTSKYKNIYERQFWNLRY 60

Query: 61  ISDAAYINTVKVKIYPPNTEIYINDRCGRFGFLYGNYDGLDRLPIFNLYVGQSVWDSVEF 120
            S     N   V I    T+  I     R  FLYGNYDG +RLP FNLYVGQS+W SVEF
Sbjct: 61  FSTGTR-NCYNVSI-NRGTKYLI-----RASFLYGNYDGFNRLPKFNLYVGQSLWGSVEF 120

Query: 121 TEVDIDVWKEIIHIPLTDNIYICLINTNQGTPFISALEFRPLPNSTYTTTAGSLSLVSRA 180
           TEVD+ +WK+IIHIPLTDNIYICLIN NQGTPFISALEFRPLPN+TYTTT GSLSL SRA
Sbjct: 121 TEVDLYLWKDIIHIPLTDNIYICLININQGTPFISALEFRPLPNTTYTTTGGSLSLYSRA 180

Query: 181 NFGTDRTYRFGYDVFDRIWTPFTYHEWTNISTSNTVDLTNHDIYEVPSIVMSTAATPTNS 240
           NFGTD+TYRFGYDVFDRIW PFTY +WTN+STSNTVD TNH IYEVPSIVMSTAATPTNS
Sbjct: 181 NFGTDQTYRFGYDVFDRIWLPFTYKKWTNVSTSNTVDPTNHAIYEVPSIVMSTAATPTNS 240

Query: 241 SSSLEFKWNSDEVTPQYYAYMYSAEIVKLQANQTRVFNVNLNGQHLFGPLSPIYLSTRTF 300
           SS LEFKWN DEVT QYYAYM  AEIVKLQANQTRVFNVNLNGQHLFGPLSPIYLST+TF
Sbjct: 241 SSPLEFKWNPDEVTSQYYAYMSFAEIVKLQANQTRVFNVNLNGQHLFGPLSPIYLSTKTF 300

Query: 301 YSETALYDPDGKYVVSLASTENSTLPPLINAFEVYKVIYLSDLQTDPAD----------- 360
           YSETALYD +G+Y +SL STENSTLPPLINAFEVYKVI +SDL+TD AD           
Sbjct: 301 YSETALYDANGQYEISLVSTENSTLPPLINAFEVYKVINISDLRTDTADVNVIMSIKLTY 360

Query: 361 ------------------------------------------------------------ 420
                                                                       
Sbjct: 361 GVNKDWQADPCLPKAYPWSGLICNNDTAPRIISLDLSSSDLTGEISPYIFNLSMLQTLDL 420

Query: 421 ------GKVPDFLVNMSSLKVIKLDKNNLTSPLPPELLKRVNNDSLTLSVEDNPNLDSAC 480
                 GKVPDFL NM SLKVIKLDKNNLT PLPPELLKRVNN+SLTLSVEDNPNLD AC
Sbjct: 421 SNNSLSGKVPDFLANMPSLKVIKLDKNNLTGPLPPELLKRVNNNSLTLSVEDNPNLDGAC 480

Query: 481 ASNSCSEEKKKSFVVPVVAPIGGLLVILTVAAVIFWIIISWNKPHGQTTVPVPRVKDTEC 540
           AS+SC+E+KKKSF+VPVVA +GGLLV+LTVAA+IFWI+   NK    +TVPVPRVKDT+ 
Sbjct: 481 ASDSCAEKKKKSFIVPVVASVGGLLVVLTVAAIIFWIVKLRNKSQDHSTVPVPRVKDTKS 540

Query: 541 GNDSLEVRRRQFTFTEVMRMTDNFARPLGEGGSGKVYLGHIDNVQVAVKMLSPLSAHSYQ 600
            NDSLEVRRRQFT++E++RMT+NFARP+GEGGSG+VYLGHI+NVQVAVKMLSPLSAHSYQ
Sbjct: 541 NNDSLEVRRRQFTYSEIVRMTENFARPVGEGGSGRVYLGHIENVQVAVKMLSPLSAHSYQ 600

Query: 601 QFQTEVKLLMRVYHGNLTSLVGYLNEKNHFGLIYEYMTKGNLPQVLSEDAVLLTRENRLQ 660
           QFQTEVKLLMRVYHGNLTSLVGYLNEK+HFGLIYEYMTKGNL QVLSE  VLLT E+RL+
Sbjct: 601 QFQTEVKLLMRVYHGNLTSLVGYLNEKDHFGLIYEYMTKGNLAQVLSESPVLLTWEDRLR 660

Query: 661 IAIGAAQ---------------------------------------GLEYLRHGCKPPIV 720
           IAI AAQ                                       GLEYL HGCKPPIV
Sbjct: 661 IAIDAAQGEKTQQNYRVYSVLSTNTLLLTFLLHHKNEILSQILKNTGLEYLHHGCKPPIV 720

Query: 721 HRDVKTTNILLTDDFQAKLADFGLSKSFPKDDSKTHMSTMVAGTPDYFDPEKRSHNSMGD 751
           HRDVKTTNILLTD+FQAKLADFGLSKSFP D SKTHMST VAGTP YFDPE  + N + +
Sbjct: 721 HRDVKTTNILLTDNFQAKLADFGLSKSFPNDGSKTHMSTTVAGTPGYFDPEYYTSNRLTE 780

BLAST of Lag0003075 vs. NCBI nr
Match: XP_038898021.1 (LRR receptor-like serine/threonine-protein kinase IOS1 isoform X4 [Benincasa hispida] >XP_038898022.1 LRR receptor-like serine/threonine-protein kinase IOS1 isoform X4 [Benincasa hispida])

HSP 1 Score: 994.6 bits (2570), Expect = 4.7e-286
Identity = 534/790 (67.59%), Postives = 580/790 (73.42%), Query Frame = 0

Query: 1   MIMEILRS-AFGFLGCLAALQVLVQAQVQSSFISLDCGLPANSSYEESTTGINYISDAAY 60
           MIMEILR   FG LGCLAA+Q+LV AQ Q+ FISLDCGLP NSSYEE TTGINYISDAAY
Sbjct: 1   MIMEILRPLLFGLLGCLAAVQILVLAQDQTGFISLDCGLPENSSYEELTTGINYISDAAY 60

Query: 61  INTVK------------------VKIYPPNTEIYINDRCG-------RFGFLYGNYDGLD 120
           I+T +                  ++ +   T    N           R  FLYGNYDG +
Sbjct: 61  ISTGESQDLTSKYKNIYERQFWNLRYFSTGTRNCYNVSINRGTKYLIRASFLYGNYDGFN 120

Query: 121 RLPIFNLYVGQSVWDSVEFTEVDIDVWKEIIHIPLTDNIYICLINTNQGTPFISALEFRP 180
           RLP FNLYVGQS+W SVEFTEVD+ +WK+IIHIPLTDNIYICLIN NQGTPFISALEFRP
Sbjct: 121 RLPKFNLYVGQSLWGSVEFTEVDLYLWKDIIHIPLTDNIYICLININQGTPFISALEFRP 180

Query: 181 LPNSTYTTTAGSLSLVSRANFGTDRTYRFGYDVFDRIWTPFTYHEWTNISTSNTVDLTNH 240
           LPN+TYTTT GSLSL SRANFGTD+TYRFGYDVFDRIW PFTY +WTN+STSNTVD TNH
Sbjct: 181 LPNTTYTTTGGSLSLYSRANFGTDQTYRFGYDVFDRIWLPFTYKKWTNVSTSNTVDPTNH 240

Query: 241 DIYEVPSIVMSTAATPTNSSSSLEFKWNSDEVTPQYYAYMYSAEIVKLQANQTRVFNVNL 300
            IYEVPSIVMSTAATPTNSSS LEFKWN DEVT QYYAYM  AEIVKLQANQTRVFNVNL
Sbjct: 241 AIYEVPSIVMSTAATPTNSSSPLEFKWNPDEVTSQYYAYMSFAEIVKLQANQTRVFNVNL 300

Query: 301 NGQHLFGPLSPIYLSTRTFYSETALYDPDGKYVVSLASTENSTLPPLINAFEVYKVIYLS 360
           NGQHLFGPLSPIYLST+TFYSETALYD +G+Y +SL STENSTLPPLINAFEVYKVI +S
Sbjct: 301 NGQHLFGPLSPIYLSTKTFYSETALYDANGQYEISLVSTENSTLPPLINAFEVYKVINIS 360

Query: 361 DLQTDPAD---------------------------------------------------- 420
           DL+TD AD                                                    
Sbjct: 361 DLRTDTADVNVIMSIKLTYGVNKDWQADPCLPKAYPWSGLICNNDTAPRIISLDLSSSDL 420

Query: 421 -------------------------GKVPDFLVNMSSLKVIKLDKNNLTSPLPPELLKRV 480
                                    GKVPDFL NM SLKVIKLDKNNLT PLPPELLKRV
Sbjct: 421 TGEISPYIFNLSMLQTLDLSNNSLSGKVPDFLANMPSLKVIKLDKNNLTGPLPPELLKRV 480

Query: 481 NNDSLTLSVEDNPNLDSACASNSCSEEKKKSFVVPVVAPIGGLLVILTVAAVIFWIIISW 540
           NN+SLTLSVEDNPNLD ACAS+SC+E+KKKSF+VPVVA +GGLLV+LTVAA+IFWI+   
Sbjct: 481 NNNSLTLSVEDNPNLDGACASDSCAEKKKKSFIVPVVASVGGLLVVLTVAAIIFWIVKLR 540

Query: 541 NKPHGQTTVPVPRVKDTECGNDSLEVRRRQFTFTEVMRMTDNFARPLGEGGSGKVYLGHI 600
           NK    +TVPVPRVKDT+  NDSLEVRRRQFT++E++RMT+NFARP+GEGGSG+VYLGHI
Sbjct: 541 NKSQDHSTVPVPRVKDTKSNNDSLEVRRRQFTYSEIVRMTENFARPVGEGGSGRVYLGHI 600

Query: 601 DNVQVAVKMLSPLSAHSYQQFQTEVKLLMRVYHGNLTSLVGYLNEKNHFGLIYEYMTKGN 649
           +NVQVAVKMLSPLSAHSYQQFQTEVKLLMRVYHGNLTSLVGYLNEK+HFGLIYEYMTKGN
Sbjct: 601 ENVQVAVKMLSPLSAHSYQQFQTEVKLLMRVYHGNLTSLVGYLNEKDHFGLIYEYMTKGN 660

BLAST of Lag0003075 vs. NCBI nr
Match: XP_023521501.1 (LRR receptor-like serine/threonine-protein kinase IOS1 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 719.5 bits (1856), Expect = 2.9e-203
Identity = 427/897 (47.60%), Postives = 553/897 (61.65%), Query Frame = 0

Query: 2   IMEI--LRSAFGFLGCLAALQVLVQAQVQSSFISLDCGLPANSSYEESTTGINYISDAAY 61
           IME+    S FGFL C   L +LVQAQ QS FISLDCG P ++SY E+TT I+Y+SDA +
Sbjct: 3   IMELSGFHSLFGFLLCGLTLLLLVQAQDQSGFISLDCGTPESTSYTETTTKIDYVSDAPF 62

Query: 62  INT------------------VKVKIYPPNTEIYINDRCG-------RFGFLYGNYDGLD 121
           IN+                   K++ +P       N           R  FLYGNYDGL+
Sbjct: 63  INSGVSGSVASIYGDIYQRQMRKLRSFPEGIRNCYNVSVKKDTKYLIRANFLYGNYDGLN 122

Query: 122 RLPIFNLYVGQSVWDSVEFTEVDIDVWKEIIHIPLTDNIYICLINTNQGTPFISALEFRP 181
            LP F+LY+G S+W+++ FT+V ID +K++IH+  +  ++ICLINT  G PFISALEFRP
Sbjct: 123 LLPQFDLYIGNSLWETINFTKVGIDTFKDLIHVTSSKEVHICLINTGNGVPFISALEFRP 182

Query: 182 LPNSTYTTTAGSLSLVSRANFGT--DRTYRFGYDVFDRIWTPFTYHEWTNISTSNTVDLT 241
           L N TY T +GSLSL +R + G+  ++TYRF +DV+DR+W+PF ++EWT +ST+ TVD T
Sbjct: 183 LLNITYQTASGSLSLYTRLDIGSTNNQTYRFPFDVYDRLWSPFNFNEWTQVSTNQTVDAT 242

Query: 242 NHDIYEVPSIVMSTAATPTNSSSSLEFKWNSDEVTPQYYAYMYSAEIVKLQANQTRVFNV 301
           +H+ ++ PS+VM TA+TP N+S  LE  W++   + QYY +++ AE+V+L+ NQ+R FN+
Sbjct: 243 DHNNHQPPSVVMKTASTPINASKPLEIWWDTVN-SSQYYVFIHFAEVVELRGNQSRGFNI 302

Query: 302 NLNGQHLFGPLSPIYLSTRTFYSETALYDPDGKYVVSLASTENSTLPPLINAFEVYKVIY 361
             NG H +GPL P YLST T YS   L D   ++  SL   EN+TLPP+INAFE+Y V  
Sbjct: 303 MHNGDHFYGPLIPSYLSTLTVYSREQL-DAADRHTFSLVPIENATLPPIINAFEIYTVKD 362

Query: 362 LSDLQTDPAD-------------------------------------------------- 421
           +S+L +D  D                                                  
Sbjct: 363 ISELASDKGDVDAITNIKSTYGVTKDWQGDPCVPMEYPWSGLNCSNEAAPRITSLNLSTS 422

Query: 422 ---------------------------GKVPDFLVNMSSLKVIKLDKNNLTSPLPPELLK 481
                                      GKVPDFL ++  LK++ L  NNL+ PLP +LLK
Sbjct: 423 GLNGEISSYISKLTMLQTLDLSNNNLTGKVPDFLSSLLHLKILNLGNNNLSGPLPADLLK 482

Query: 482 RVNNDSLTLSVEDNPNLDSACASNSC-SEEKKKSFVVPVVAPIGGLLVILTVAAVIFWII 541
           R N+ SL+LSV  N NLD AC S+ C   EKK + ++P+VA IGG+LVI+T+AA+ FWII
Sbjct: 483 RSNDGSLSLSVGGNQNLD-ACTSDPCPKNEKKNNIIIPIVASIGGVLVIVTIAAISFWII 542

Query: 542 ISWNKPHGQTTVPVPRVKDT--ECGNDSLEVRRRQFTFTEVMRMTDNFARPLGEGGSGKV 601
            S  KP G+  V V    +T  + GN SLEVRR+QFT++EV++MT+NF + LG+GG G+V
Sbjct: 543 KSRKKPQGKNVVSVVDKSETNSQFGN-SLEVRRQQFTYSEVVKMTNNFTKVLGKGGFGEV 602

Query: 602 YLGHIDNVQVAVKMLSPLSAHSYQQFQTEVKLLMRVYHGNLTSLVGYLNEKNHFGLIYEY 661
           Y G ID++QVAVKMLS  S+  Y+QFQ EV LLMRV+H NLTSLVGYLNE NH GLIYEY
Sbjct: 603 YYGVIDDIQVAVKMLSLSSSQGYRQFQAEVTLLMRVHHRNLTSLVGYLNEGNHLGLIYEY 662

Query: 662 MTKGNLPQVLSEDA-VLLTRENRLQIAIGAAQGLEYLRHGCKPPIVHRDVKTTNILLTDD 721
           M  G+L + LSE +  +L+ E+RL+IAI AAQGLEYL +GCKPPIVHRDVKTTNILLTD+
Sbjct: 663 MANGDLAEHLSESSPSILSWEDRLRIAIDAAQGLEYLHYGCKPPIVHRDVKTTNILLTDN 722

Query: 722 FQAKLADFGLSKSFPKDDSKTHMSTMVAGTPDYFDPEKRSHNSMGDR------------- 751
           FQ KLADFGLSKSFP  D  THMST+VAGTP Y DPE    N + ++             
Sbjct: 723 FQGKLADFGLSKSFP-IDGNTHMSTVVAGTPGYLDPEYYVSNRLTEKSDVYSFGIALLEI 782

BLAST of Lag0003075 vs. ExPASy Swiss-Prot
Match: Q9C8I6 (LRR receptor-like serine/threonine-protein kinase IOS1 OS=Arabidopsis thaliana OX=3702 GN=IOS1 PE=1 SV=1)

HSP 1 Score: 486.9 bits (1252), Expect = 4.2e-136
Identity = 334/894 (37.36%), Postives = 462/894 (51.68%), Query Frame = 0

Query: 9   AFGFLGCLAALQV-----LVQAQVQSSFISLDCGLPANSSYEESTTGINYISDAAYIN-- 68
           AF     L  LQ+     L  AQ QS FISLDCG P  +S+ E TT I YISDA +IN  
Sbjct: 2   AFSSCFLLVLLQIFSALLLCLAQDQSGFISLDCGSPRETSFREKTTNITYISDANFINTG 61

Query: 69  ----------------TVKVKIYPP------NTEIYINDR-CGRFGFLYGNYDGLDRLPI 128
                           T  ++ +P          + I D    R  FL+G YD       
Sbjct: 62  VGGSIKQGYRTQFQQQTWNLRSFPQGIRNCYTLNLTIGDEYLIRANFLHGGYDDKPSTQ- 121

Query: 129 FNLYVGQSVWDSVEFT-EVDIDVWKEIIHIPLTDNIYICLINTNQGTPFISALEFRPLPN 188
           F LY+G ++W +V  T E +  ++ E+IHI  TD + ICL+ T   TPFISALE R L N
Sbjct: 122 FELYLGPNLWSTVTTTNETEASIF-EMIHILTTDRLQICLVKTGNATPFISALELRKLMN 181

Query: 189 STYTTTAGSLSLVSRANFG--TDRTYRFGYDVFDRIWTPFTYHEWTNISTSNTVDLTNHD 248
           +TY T  GSL    RA+ G   ++ YR+G DVFDR+WTP+ +  W+ IST+ +V++ N  
Sbjct: 182 TTYLTRQGSLQTFIRADVGATVNQGYRYGIDVFDRVWTPYNFGNWSQISTNQSVNINND- 241

Query: 249 IYEVPSIVMSTAATPTNSSSSLEFKWNSDEVTPQYYAYMYSAEIVKLQANQTRVFNVNLN 308
            Y+ P I M TA+ PT+  +++       E T Q+Y +M+ AEI +L++N TR FN+  N
Sbjct: 242 -YQPPEIAMVTASVPTDPDAAMNISLVGVERTVQFYVFMHFAEIQELKSNDTREFNIMYN 301

Query: 309 GQHLFGPLSPIYLSTRTFYSET-ALYDPDGKYVVSLASTENSTLPPLINAFEVYKVIYLS 368
            +H++GP  P+  +T + ++ T  + D +G+Y+ SL  T NSTLPPL+NA E+Y V  L 
Sbjct: 302 NKHIYGPFRPLNFTTSSVFTPTEVVADANGQYIFSLQRTGNSTLPPLLNAMEIYSVNLLP 361

Query: 369 DLQTD---------------------------PAD------------------------- 428
             +TD                           P D                         
Sbjct: 362 QQETDRKEVDAMMNIKSAYGVNKIDWEGDPCVPLDYKWSGVNCTYVDNETPKIISLDLST 421

Query: 429 ----------------------------GKVPDFLVNMSSLKVIKLDKNNLTSPLPPELL 488
                                       G VP+FL NM +LK+I L  N L   +P  LL
Sbjct: 422 SGLTGEILEFISDLTSLEVLDLSNNSLTGSVPEFLANMETLKLINLSGNELNGSIPATLL 481

Query: 489 KRVNNDSLTLSVEDNPNLDSACASNSCSEEKKKSFVVPVVAPIGGLL--VILTVAAVIFW 548
            +    S+TLS+E N  L   C+S SC+  KKK     V+AP+   L  V L  A ++ +
Sbjct: 482 DKERRGSITLSIEGNTGL---CSSTSCATTKKKK-KNTVIAPVAASLVSVFLIGAGIVTF 541

Query: 549 IIISWNK-------PHGQTTVPVPRVKDTECGNDSLEVRRRQFTFTEVMRMTDNFARPLG 608
           +I+   K       P+  T       +        +  + R+ T+ +V+++T+NF R LG
Sbjct: 542 LILKRKKRTKLGLNPNSGTGTTPLHSRSHHGFEPPVIAKNRKLTYIDVVKITNNFERVLG 601

Query: 609 EGGSGKVYLGHIDNVQVAVKMLSPLSAHSYQQFQTEVKLLMRVYHGNLTSLVGYLNEKNH 668
            GG G VY G ++N  VAVKML+  +A  Y+QF+ EV+LL+RV+H +LT LVGY  E + 
Sbjct: 602 RGGFGVVYYGVLNNEPVAVKMLTESTALGYKQFKAEVELLLRVHHKDLTCLVGYCEEGDK 661

Query: 669 FGLIYEYMTKGNLPQVLS--EDAVLLTRENRLQIAIGAAQGLEYLRHGCKPPIVHRDVKT 728
             LIYE+M  G+L + LS      +LT E RL+IA  +AQGLEYL +GCKP IVHRD+KT
Sbjct: 662 MSLIYEFMANGDLKEHLSGKRGPSILTWEGRLRIAAESAQGLEYLHNGCKPQIVHRDIKT 721

Query: 729 TNILLTDDFQAKLADFGLSKSFPKDDSKTHMSTMVAGTPDYFDPE--------------- 742
           TNILL + FQAKLADFGLS+SFP   ++TH+ST+VAGTP Y DPE               
Sbjct: 722 TNILLNEKFQAKLADFGLSRSFPL-GTETHVSTIVAGTPGYLDPEYYRTNWLTEKSDVFS 781

BLAST of Lag0003075 vs. ExPASy Swiss-Prot
Match: Q9FZB1 (Probable LRR receptor-like serine/threonine-protein kinase At1g51880 OS=Arabidopsis thaliana OX=3702 GN=At1g51880 PE=2 SV=1)

HSP 1 Score: 471.1 bits (1211), Expect = 2.4e-131
Identity = 323/895 (36.09%), Postives = 459/895 (51.28%), Query Frame = 0

Query: 6   LRSAFGFLGCLAALQVL---VQAQVQSSFISLDCGL-PANSSYEESTTGINYISDAAYIN 65
           ++S  GFL  L    V+   VQAQ Q  FISLDCGL P N++Y E TT I Y SDA YI+
Sbjct: 1   MKSIHGFLLFLITAYVILESVQAQDQLGFISLDCGLVPKNATYTEKTTNITYKSDANYID 60

Query: 66  ------------------TVKVKIYPPNTEIYINDRCG-------RFGFLYGNYDGLDRL 125
                             T  V+ +P       N           R  F YGNYDGL ++
Sbjct: 61  SGLVGRISAEYKAQLQQQTWTVRSFPEGERNCYNFNLTAKSRYLIRATFTYGNYDGLRQV 120

Query: 126 PIFNLYVGQSVWDSVEFTEVDIDVWKEIIHIPLTDNIYICLINTNQGTPFISALEFRPLP 185
           P F++++G S W SV+   V      E+IH+   D + ICL+ T +G PFIS+LE RPL 
Sbjct: 121 PKFDIHIGPSKWTSVKLDGVGNGAVLEMIHVLTQDRLQICLVKTGKGIPFISSLELRPLN 180

Query: 186 NSTYTTTAGSLSLVSRANFGTDRTY-RFGYDVFDRIWTPFTYHEWTNISTSNTVDLTNHD 245
           N+TY T +GSL   +R  F    T+ R+  D+ DR+W     +   +IST   VD +N  
Sbjct: 181 NNTYLTQSGSLIGFARVFFSATPTFIRYDEDIHDRVWVRQFGNGLKSISTDLLVDTSNP- 240

Query: 246 IYEVPSIVMSTAATPTNSSSSLEFKWNSDEVTPQYYAYMYSAEIVKLQANQTRVFNVNLN 305
            Y+VP  V  TA  P+N+S  L F W  D +T Q Y YM+ AEI  L+ N  R FN+  N
Sbjct: 241 -YDVPQAVAKTACVPSNASQPLIFDWTLDNITSQSYVYMHFAEIQTLKDNDIREFNITYN 300

Query: 306 -GQHLFGPLSPIYLSTRTFYSETALYDPDGKYVVSLASTENSTLPPLINAFEVYKVIYLS 365
            GQ+++  L P      T +    L  PDG + +S   T NSTLPPLIN  E+YKV+ L 
Sbjct: 301 GGQNVYSYLRPEKFEISTLFDSKPLSSPDGSFSLSFTKTGNSTLPPLINGLEIYKVLDLL 360

Query: 366 DLQTDPAD---------------------------------------------------- 425
           +L+TD  +                                                    
Sbjct: 361 ELETDQDEVSAMINIKATYDLSKKVSWQGDPCAPKSYQWEGLNCSYPNSDQPRIISLNLA 420

Query: 426 -----------------------------GKVPDFLVNMSSLKVIKLDKN-NLTSPLPPE 485
                                        G++P+F  +M  LK+I L  N  L S +P  
Sbjct: 421 ENKLTGTITPEISKLTQLIELDLSKNDLSGEIPEFFADMKLLKLINLSGNLGLNSTIPDS 480

Query: 486 LLKRVNNDSLTLSVEDNPNLDSACASNSCSEEKKKSFVVPVVAPIGGLLVILTVAAVIFW 545
           + +R+++ SL L +       +   + +   + KK  ++P+VA + G+  +L + A+ F 
Sbjct: 481 IQQRLDSKSLILILS-----KTVTKTVTLKGKSKKVPMIPIVASVAGVFALLVILAIFFV 540

Query: 546 IIISWNKPHGQTTVPVPRVKDTECGNDSLEVRRRQFTFTEVMRMTDNFARPLGEGGSGKV 605
           +     + +G++       K T   N S+  + R+ T+ EV++MT+NF R LG+GG G V
Sbjct: 541 V----RRKNGESN------KGT---NPSIITKERRITYPEVLKMTNNFERVLGKGGFGTV 600

Query: 606 YLGHIDNVQVAVKMLSPLSAHSYQQFQTEVKLLMRVYHGNLTSLVGYLNEKNHFGLIYEY 665
           Y G++++ QVAVKMLS  SA  Y++F+ EV+LL+RV+H NL  LVGY ++ ++  LIYEY
Sbjct: 601 YHGNLEDTQVAVKMLSHSSAQGYKEFKAEVELLLRVHHRNLVGLVGYCDDGDNLALIYEY 660

Query: 666 MTKGNLPQVLS--EDAVLLTRENRLQIAIGAAQGLEYLRHGCKPPIVHRDVKTTNILLTD 725
           M  G+L + +S      +LT ENR+QIA+ AAQGLEYL +GC PP+VHRDVKTTNILL +
Sbjct: 661 MANGDLKENMSGKRGGNVLTWENRMQIAVEAAQGLEYLHNGCTPPMVHRDVKTTNILLNE 720

Query: 726 DFQAKLADFGLSKSFPKDDSKTHMSTMVAGTPDYFDPEKRSHNSMGDRHYCAR------- 751
            + AKLADFGLS+SFP  D ++H+ST+VAGTP Y DPE    N + ++            
Sbjct: 721 RYGAKLADFGLSRSFPV-DGESHVSTVVAGTPGYLDPEYYRTNWLSEKSDVYSFGVVLLE 780

BLAST of Lag0003075 vs. ExPASy Swiss-Prot
Match: C0LGG4 (Probable LRR receptor-like serine/threonine-protein kinase At1g51860 OS=Arabidopsis thaliana OX=3702 GN=At1g51860 PE=2 SV=2)

HSP 1 Score: 470.7 bits (1210), Expect = 3.1e-131
Identity = 322/874 (36.84%), Postives = 457/874 (52.29%), Query Frame = 0

Query: 23  VQAQVQSSFISLDCGL-PANSSYEESTTGINYISDAAYINT------------------V 82
           V+AQ Q+ FISLDCGL P  ++Y E +T I Y SD  YI++                   
Sbjct: 21  VEAQNQAGFISLDCGLVPKETTYTEKSTNITYKSDVDYIDSGLVGKINDAYKTQFQQQVW 80

Query: 83  KVKIYPP------NTEIYINDR-CGRFGFLYGNYDGLDRLPIFNLYVGQSVWDSVEFTEV 142
            V+ +P       N  +  N++   R  F+YGNYDGL++ P F+L++G + W SV+   V
Sbjct: 81  AVRSFPVGQRNCYNVNLTANNKYLIRGTFVYGNYDGLNQFPSFDLHIGPNKWSSVKILGV 140

Query: 143 DIDVWKEIIHIPLTDNIYICLINTNQGTPFISALEFRPLPNSTYTTTAGSLSLVSRANFG 202
                 EIIH+   D++ +CL+ T   TPFIS+LE RPL N +Y T +GSL L +R  F 
Sbjct: 141 TNTSMHEIIHVVPQDSLEVCLVKTGPTTPFISSLEVRPLNNESYLTQSGSLMLFARVYFP 200

Query: 203 TDRT--YRFGYDVFDRIWTPFTYHEWTNISTSNTVDLTNHDIYEVPSIVMSTAATPTNSS 262
           +  +   R+  D+ DR+W  FT  E   IST   +D +N   Y++P  VM TAA P N+S
Sbjct: 201 SSSSSFIRYDEDIHDRVWNSFTDDETVWISTDLPIDTSNS--YDMPQSVMKTAAVPKNAS 260

Query: 263 SSLEFKWNSDEVTPQYYAYMYSAEIVKLQANQTRVFNVNLN-GQHLFGPLSPIYLSTRTF 322
                 W  DE T Q Y YM+ AE+  L AN+TR FN+  N G   F  L P  LS  T 
Sbjct: 261 EPWLLWWTLDENTAQSYVYMHFAEVQNLTANETREFNITYNGGLRWFSYLRPPNLSISTI 320

Query: 323 YSETALYDPDGKYVVSLASTENSTLPPLINAFEVYKVIYLSDLQT--------------- 382
           ++  A+   +G +  + A T NSTLPPL+NA E+Y V+ +  L+T               
Sbjct: 321 FNPRAVSSSNGIFNFTFAMTGNSTLPPLLNALEIYTVVDILQLETNKDEVSAMMNIKETY 380

Query: 383 ----------DPA----------------------------------------------- 442
                     DP                                                
Sbjct: 381 GLSKKISWQGDPCAPQLYRWEGLNCSYPDSEGSRIISLNLNGSELTGSITSDISKLTLLT 440

Query: 443 ---------DGKVPDFLVNMSSLKVIKLDKN---NLTSPLPPELLKRVNNDSLTLSVEDN 502
                     G +P F   M SLK+I L  N   NLT+ +P  L +RVN+ SLTL + +N
Sbjct: 441 VLDLSNNDLSGDIPTFFAEMKSLKLINLSGNPNLNLTA-IPDSLQQRVNSKSLTLILGEN 500

Query: 503 PNLDSACASNSCSEEKKKSFVVPVVAPIGGLLVILTVAAVIFWI---IISWNKPHGQTTV 562
             L       +  +E KK  +V + A + G+  +L + A+ F I    +  +K  G   +
Sbjct: 501 LTL-------TPKKESKKVPMVAIAASVAGVFALLVILAIFFVIKRKNVKAHKSPGPPPL 560

Query: 563 PVPRV--KDTECGNDSLEVRRRQFTFTEVMRMTDNFARPLGEGGSGKVYLGHIDNVQVAV 622
             P +   +T   N S+  R R+ T+ EV++MT+NF R LG+GG G VY G++D  +VAV
Sbjct: 561 VTPGIVKSETRSSNPSIITRERKITYPEVLKMTNNFERVLGKGGFGTVYHGNLDGAEVAV 620

Query: 623 KMLSPLSAHSYQQFQTEVKLLMRVYHGNLTSLVGYLNEKNHFGLIYEYMTKGNLPQVLS- 682
           KMLS  SA  Y++F+ EV+LL+RV+H +L  LVGY ++ ++  LIYEYM  G+L + +S 
Sbjct: 621 KMLSHSSAQGYKEFKAEVELLLRVHHRHLVGLVGYCDDGDNLALIYEYMANGDLRENMSG 680

Query: 683 -EDAVLLTRENRLQIAIGAAQGLEYLRHGCKPPIVHRDVKTTNILLTDDFQAKLADFGLS 742
                +LT ENR+QIA+ AAQGLEYL +GC+PP+VHRDVKTTNILL +   AKLADFGLS
Sbjct: 681 KRGGNVLTWENRMQIAVEAAQGLEYLHNGCRPPMVHRDVKTTNILLNERCGAKLADFGLS 740

BLAST of Lag0003075 vs. ExPASy Swiss-Prot
Match: C0LGG6 (Probable LRR receptor-like protein kinase At1g51890 OS=Arabidopsis thaliana OX=3702 GN=At1g51890 PE=1 SV=2)

HSP 1 Score: 468.8 bits (1205), Expect = 1.2e-130
Identity = 320/878 (36.45%), Postives = 450/878 (51.25%), Query Frame = 0

Query: 12  FLGCLAALQVLVQAQVQSSFISLDCGL-PANSSYEESTTGINYISDAAYINT-VKVKIYP 71
           FL  + A+  LVQAQ QS FISLDCGL P   +Y E +T I Y SDA YI++ V  KI  
Sbjct: 6   FLIFVFAVLGLVQAQDQSGFISLDCGLVPTEITYVEKSTNITYRSDATYIDSGVPGKI-- 65

Query: 72  PNTEIYIND----------------RCGRFG------------FLYGNYDGLDRLPIFNL 131
              E+Y                    C  F             F+YGNYDGL++LP F+L
Sbjct: 66  --NEVYRTQFQQQIWALRSFPEGQRNCYNFSLTAKRKYLIRGTFIYGNYDGLNQLPSFDL 125

Query: 132 YVGQSVWDSVEFTEVDIDVWKEIIHIPLTDNIYICLINTNQGTPFISALEFRPLPNSTYT 191
           Y+G + W SV    V      E+IH+   D++ ICL+ T + TPFIS+LE RPL N+TY 
Sbjct: 126 YIGPNKWTSVSIPGVRNGSVSEMIHVLRQDHLQICLVKTGETTPFISSLELRPLNNNTYV 185

Query: 192 TTAGSLSLVSRANFG-TDRTYRFGYDVFDRIWTPFTYHEWTNISTSNTVDLTNHDIYEVP 251
           T +GSL +V+R  F  T    R+  DV DRIW PF  ++ + +ST  +VD +N   Y VP
Sbjct: 186 TKSGSLIVVARLYFSPTPPFLRYDEDVHDRIWIPFLDNKNSLLSTELSVDTSN--FYNVP 245

Query: 252 SIVMSTAATPTNSSSSLEFKWNSDEVTPQYYAYMYSAEIVKLQANQTRVFNVNLN-GQHL 311
             V  TAA P N++  L+  W+ D++T Q Y YM+ AEI  L+AN+TR FN+  N G++ 
Sbjct: 246 QTVAKTAAVPLNATQPLKINWSLDDITSQSYIYMHFAEIENLEANETREFNITYNGGENW 305

Query: 312 FGPLSPIYLSTRTFYSETALYDPDGKYVVSLASTENSTLPPLINAFEVYKVIYLSDLQT- 371
           F    P      T Y+  A+   DG +  + + T NST PPLIN  E+Y+V+ L  L T 
Sbjct: 306 FSYFRPPKFRITTVYNPAAVSSLDGNFNFTFSMTGNSTHPPLINGLEIYQVLELPQLDTY 365

Query: 372 ------------------------DPA--------------------------------- 431
                                   DP                                  
Sbjct: 366 QDEVSAMMNIKTIYGLSKRSSWQGDPCAPELYRWEGLNCSYPNFAPPQIISLNLSGSNLS 425

Query: 432 -----------------------DGKVPDFLVNMSSLKVIKLDKN-NLTSPLPPELLKRV 491
                                   G +P    +M +L +I L  N NL   +P  L KR+
Sbjct: 426 GTITSDISKLTHLRELDLSNNDLSGDIPFVFSDMKNLTLINLSGNKNLNRSVPETLQKRI 485

Query: 492 NNDSLTLSVEDNPNLDSACASNSCSEEKKKSFVVPVVAPIGGLLVILTVAAVIFWIIIS- 551
           +N SLTL  ++                K  + VV + A +  +  +L + A++F +I   
Sbjct: 486 DNKSLTLIRDET--------------GKNSTNVVAIAASVASVFAVLVILAIVFVVIRKK 545

Query: 552 --WNKPHGQTTVPVPRVK-DTECGNDSLEVRRRQFTFTEVMRMTDNFARPLGEGGSGKVY 611
              N+  G  +     VK D    + S+  + R+FT++EV++MT NF R LG+GG G VY
Sbjct: 546 QRTNEASGPRSFTTGTVKSDARSSSSSIITKERKFTYSEVLKMTKNFERVLGKGGFGTVY 605

Query: 612 LGHIDNVQVAVKMLSPLSAHSYQQFQTEVKLLMRVYHGNLTSLVGYLNEKNHFGLIYEYM 671
            G++D+ QVAVKMLS  SA  Y++F+ EV+LL+RV+H +L  LVGY ++ ++  LIYEYM
Sbjct: 606 HGNLDDTQVAVKMLSHSSAQGYKEFKAEVELLLRVHHRHLVGLVGYCDDGDNLALIYEYM 665

Query: 672 TKGNLPQVLS--EDAVLLTRENRLQIAIGAAQGLEYLRHGCKPPIVHRDVKTTNILLTDD 731
            KG+L + +S      +L+ E R+QIA+ AAQGLEYL +GC+PP+VHRDVK TNILL + 
Sbjct: 666 EKGDLRENMSGKHSVNVLSWETRMQIAVEAAQGLEYLHNGCRPPMVHRDVKPTNILLNER 725

Query: 732 FQAKLADFGLSKSFPKDDSKTHMSTMVAGTPDYFDPEKRSHNSMGDR------------- 735
            QAKLADFGLS+SFP  D ++H+ T+VAGTP Y DPE    N + ++             
Sbjct: 726 SQAKLADFGLSRSFPV-DGESHVMTVVAGTPGYLDPEYYRTNWLSEKSDVYSFGVVLLEI 785

BLAST of Lag0003075 vs. ExPASy Swiss-Prot
Match: Q9FN93 (Probable LRR receptor-like serine/threonine-protein kinase At5g59680 OS=Arabidopsis thaliana OX=3702 GN=At5g59680 PE=1 SV=1)

HSP 1 Score: 467.2 bits (1201), Expect = 3.4e-130
Identity = 313/862 (36.31%), Postives = 441/862 (51.16%), Query Frame = 0

Query: 24  QAQVQSSFISLDCGLPAN--SSYEESTTGINYISDAAYINTVKVK----------IYPPN 83
           QAQ Q  FISLDCGLPAN  S Y E  TG+ + SDAA+I + K+           + P  
Sbjct: 22  QAQSQQGFISLDCGLPANEPSPYTEPRTGLQFSSDAAFIQSGKIGRIQANLEADFLKPST 81

Query: 84  TEIYIND---------------RCGRFGFLYGNYDGLDRLPIFNLYVGQSVWDSVEFTEV 143
           T  Y  D                  R  F+YGNYDG D  P F+LY+G + W +++  + 
Sbjct: 82  TMRYFPDGKRNCYNLNVEKGRNHLIRARFVYGNYDGRDTGPKFDLYLGPNPWATIDLAKQ 141

Query: 144 DIDVWKEIIHIPLTDNIYICLINTNQGTPFISALEFRPLPNSTYTTTAGSLSLVSRANFG 203
                 EI+HIP ++ + +CL+ T + TP IS LE RP+ + TY T +GSL L  R  F 
Sbjct: 142 VNGTRPEIMHIPTSNKLQVCLVKTGETTPLISVLEVRPMGSGTYLTKSGSLKLYYREYFS 201

Query: 204 -TDRTYRFGYDVFDRIWTPFTYHEWTNISTSNTVDLTNHDIYEVPSIVMSTAATPTNSSS 263
            +D + R+  D++DR WT F   EWT I+T  T D+ N + Y+ P + ++TAA PTN+S+
Sbjct: 202 KSDSSLRYPDDIYDRQWTSFFDTEWTQINT--TSDVGNSNDYKPPKVALTTAAIPTNASA 261

Query: 264 SLEFKWNSDEVTPQYYAYMYSAEIVKLQANQTRVFNVNLNGQHLFGPLSPIYLSTRTFYS 323
            L  +W+S     QYY Y + +EI +LQAN+TR FN+ LNG+  FGP+ P  L+  T  S
Sbjct: 262 PLTNEWSSVNPDEQYYVYAHFSEIQELQANETREFNMLLNGKLFFGPVVPPKLAISTILS 321

Query: 324 ETALYDPDGKYVVSLASTENSTLPPLINAFEVYKVIYLSDLQTDPAD------------- 383
            +      G+  + L  T  STLPPL+NA+EVYKVI    L+T+  D             
Sbjct: 322 VSPNTCEGGECNLQLIRTNRSTLPPLLNAYEVYKVIQFPQLETNETDVSAVKNIQATYEL 381

Query: 384 ------------------------------------------------------------ 443
                                                                       
Sbjct: 382 SRINWQSDPCVPQQFMWDGLNCSITDITTPPRITTLNLSSSGLTGTITAAIQNLTTLEKL 441

Query: 444 --------GKVPDFLVNMSSLKVIKLDKNNLTSPLPPELLKRVNNDSLTLSVEDNPNLDS 503
                   G+VP+FL NM SL VI L  N+L   +P  L ++     L L  + NP L S
Sbjct: 442 DLSNNNLTGEVPEFLSNMKSLLVINLSGNDLNGTIPQSLQRK----GLELLYQGNPRLIS 501

Query: 504 ACASNSCSEEKKKSFVVPVVAPIGGLLVILTVAAVIFWIIISWNKPHGQTTV------PV 563
                S   +  KSF V +VA +G   +++ V  ++  + +   KP     V      P 
Sbjct: 502 ---PGSTETKSGKSFPVTIVASVGSAAILIVVLVLV--LFLRKKKPSAVEVVLPRPSRPT 561

Query: 564 PRVKDTECGNDSLEVRRRQFTFTEVMRMTDNFARPLGEGGSGKVYLGHID-NVQVAVKML 623
             V        S+E+++R+FT++EV +MT+NF R +GEGG G V  G ++ + QVAVK+L
Sbjct: 562 MNVPYANSPEPSIEMKKRKFTYSEVTKMTNNFGRVVGEGGFGVVCHGTVNGSEQVAVKLL 621

Query: 624 SPLSAHSYQQFQTEVKLLMRVYHGNLTSLVGYLNEKNHFGLIYEYMTKGNLPQVLSEDA- 683
           S  S   Y++F+ EV LL+RV+H NL SLVGY +E +H  LIYE++  G+L Q LS    
Sbjct: 622 SQSSTQGYKEFKAEVDLLLRVHHTNLVSLVGYCDEGDHLALIYEFVPNGDLRQHLSGKGG 681

Query: 684 -VLLTRENRLQIAIGAAQGLEYLRHGCKPPIVHRDVKTTNILLTDDFQAKLADFGLSKSF 733
             ++    RL+IA  AA GLEYL  GC PP+VHRDVKTTNILL + ++AKLADFGLS+SF
Sbjct: 682 KPIVNWGTRLRIAAEAALGLEYLHIGCTPPMVHRDVKTTNILLDEHYKAKLADFGLSRSF 741

BLAST of Lag0003075 vs. ExPASy TrEMBL
Match: A0A6J1GR51 (LRR receptor-like serine/threonine-protein kinase IOS1 OS=Cucurbita moschata OX=3662 GN=LOC111456368 PE=4 SV=1)

HSP 1 Score: 714.5 bits (1843), Expect = 4.5e-202
Identity = 420/883 (47.57%), Postives = 545/883 (61.72%), Query Frame = 0

Query: 2   IMEI--LRSAFGFLGCLAALQVLVQAQVQSSFISLDCGLPANSSYEESTTGINYISDAAY 61
           IME+    S FGFL C   L +LVQAQ QS FISLDCG P ++SY E+TT INY+SDA +
Sbjct: 3   IMELSGFHSLFGFLLCGLTLLLLVQAQDQSGFISLDCGTPESTSYTETTTKINYVSDAPF 62

Query: 62  INT------------------VKVKIYPPNTEIYINDRCG-------RFGFLYGNYDGLD 121
           IN+                   K++ +P       N           R  FLYGNYDGL+
Sbjct: 63  INSGVSGSVASIYGDTYQRQMRKLRSFPEGIRNCYNVSVKKDTKYLIRANFLYGNYDGLN 122

Query: 122 RLPIFNLYVGQSVWDSVEFTEVDIDVWKEIIHIPLTDNIYICLINTNQGTPFISALEFRP 181
            LP F+LY+G S+W+++ FT+  ID +K++IH+  +  ++ICLINT  G PFISALEFRP
Sbjct: 123 LLPQFDLYIGNSLWETINFTKAGIDTFKDLIHVTSSKEVHICLINTGNGVPFISALEFRP 182

Query: 182 LPNSTYTTTAGSLSLVSRANFGT--DRTYRFGYDVFDRIWTPFTYHEWTNISTSNTVDLT 241
           L N TY T +GSLSL +R + G+  ++TYRF +DV+DR+W+PF ++EWT +ST+ TVD T
Sbjct: 183 LLNITYQTVSGSLSLYTRLDIGSTNNQTYRFPFDVYDRLWSPFNFNEWTQVSTNQTVDAT 242

Query: 242 NHDIYEVPSIVMSTAATPTNSSSSLEFKWNSDEVTPQYYAYMYSAEIVKLQANQTRVFNV 301
           +H+ ++ PS+VM TA+TP N+S  LE  W++ + + QYY +++ AE+V+L+ NQ+R FN+
Sbjct: 243 DHNNHQPPSVVMKTASTPINASKPLEIWWDTVD-SSQYYVFIHFAEVVELRGNQSRGFNI 302

Query: 302 NLNGQHLFGPLSPIYLSTRTFYSETALYDPDGKYVVSLASTENSTLPPLINAFEVYKVIY 361
             NG H +GPL P YLST T YS   L D   ++  SL   EN+TLPP+INAFE+Y V  
Sbjct: 303 MHNGDHFYGPLIPSYLSTLTVYSREQL-DAADRHTFSLVPIENATLPPIINAFEIYTVKD 362

Query: 362 LSDLQTDPAD-------------------------------------------------- 421
           +S+L +D  D                                                  
Sbjct: 363 ISELASDKGDVDAITNIKSTYGVKKDWQGDPCVPMRYPWSGLNCSNEAAPRITSLNLSTS 422

Query: 422 ---------------------------GKVPDFLVNMSSLKVIKLDKNNLTSPLPPELLK 481
                                      GKVPDFL ++  LK++ L  NNL+ P+P +LLK
Sbjct: 423 GLNGEISSYISSLTMLQTLDLSNNNLTGKVPDFLSSLLHLKILNLGNNNLSGPIPADLLK 482

Query: 482 RVNNDSLTLSVEDNPNLDSACASNSC-SEEKKKSFVVPVVAPIGGLLVILTVAAVIFWII 541
           R N+ SL+LSV  N NLD AC S+ C   EKK + ++P+VA IGG+LVI+T+ A+ FWII
Sbjct: 483 RSNDGSLSLSVGGNQNLD-ACTSDPCPKNEKKNNIIIPIVASIGGVLVIVTIGAITFWII 542

Query: 542 ISWNKPHGQTTVPVPRVKDT--ECGNDSLEVRRRQFTFTEVMRMTDNFARPLGEGGSGKV 601
            S  KP G+  V V    +T  + GN SLEVRR+QFT++EV++MT+NF R LG+GG G+V
Sbjct: 543 KSRKKPQGKNVVSVVDKSETNSQFGN-SLEVRRQQFTYSEVVKMTNNFTRVLGKGGFGEV 602

Query: 602 YLGHIDNVQVAVKMLSPLSAHSYQQFQTEVKLLMRVYHGNLTSLVGYLNEKNHFGLIYEY 661
           Y G ID++QVAVKMLS  S+  Y+QFQ EV LLMRV+H NLTSLVGYLNE NH GLIYEY
Sbjct: 603 YYGVIDDIQVAVKMLSLSSSQGYRQFQAEVTLLMRVHHRNLTSLVGYLNEGNHLGLIYEY 662

Query: 662 MTKGNLPQVLSE-DAVLLTRENRLQIAIGAAQGLEYLRHGCKPPIVHRDVKTTNILLTDD 721
           M  G+L + LSE    +L+ E+RL+IA+ AAQGLEYL +GCKPPIVHRDVKTTNILLTD+
Sbjct: 663 MANGDLAEHLSERSPSILSWEDRLRIAMDAAQGLEYLHYGCKPPIVHRDVKTTNILLTDN 722

Query: 722 FQAKLADFGLSKSFPKDDSKTHMSTMVAGTPDYFDPEKRSHNSMGDR------------- 740
           FQ KLADFGLSKSFP  D  THMST+VAGTP Y DPE    N + ++             
Sbjct: 723 FQGKLADFGLSKSFP-IDGNTHMSTVVAGTPGYLDPEYYVSNRLTEKSDVYSFGIALLEI 782

BLAST of Lag0003075 vs. ExPASy TrEMBL
Match: A0A6J1JWM5 (LRR receptor-like serine/threonine-protein kinase IOS1 OS=Cucurbita maxima OX=3661 GN=LOC111488544 PE=4 SV=1)

HSP 1 Score: 694.5 bits (1791), Expect = 4.9e-196
Identity = 415/883 (47.00%), Postives = 537/883 (60.82%), Query Frame = 0

Query: 2   IMEI--LRSAFGFLGCLAALQVLVQAQVQSSFISLDCGLPANSSYEESTTGINYISDAAY 61
           IME+    S FGFL C   L +LVQAQ QS FISLDCG P ++SY E+TT I+Y+SDA +
Sbjct: 3   IMELSGFHSLFGFLLCGLTLLLLVQAQDQSGFISLDCGTPESTSYTETTTKIDYVSDAPF 62

Query: 62  INT------------------VKVKIYPPNTEIYINDRCG-------RFGFLYGNYDGLD 121
           IN+                   K++ +P       N           R  FLYGNYDGL+
Sbjct: 63  INSGVSGNVASIYGDIYQRQMRKLRSFPQGIRNCYNVSVKRDTKYLIRASFLYGNYDGLN 122

Query: 122 RLPIFNLYVGQSVWDSVEFTEVDIDVWKEIIHIPLTDNIYICLINTNQGTPFISALEFRP 181
             P F+LY+G S+W+++  T+  ID +K++IH+  ++ ++ICLIN   G PFISALEFRP
Sbjct: 123 LPPNFDLYIGNSLWETINLTKNGIDAFKDLIHVTSSNEVHICLINKGNGVPFISALEFRP 182

Query: 182 LPNSTYTTTAGSLSLVSRANFGT--DRTYRFGYDVFDRIWTPFTYHEWTNISTSNTVDLT 241
           L N TY T +GSLSL  R + G+  ++TYRF +DV+DR W+PF ++EWT +ST+ TVD  
Sbjct: 183 LLNVTYQTASGSLSLYYRLDVGSTKNQTYRFPFDVYDRFWSPFNFNEWTQVSTNQTVDAI 242

Query: 242 NHDIYEVPSIVMSTAATPTNSSSSLEFKWNSDEVTPQYYAYMYSAEIVKLQANQTRVFNV 301
           +H+ ++ PSIVM TA+  TN+S  LE  W++ + + QYY +++ AE+V+L+ NQ+R FN+
Sbjct: 243 DHNNHQPPSIVMKTASFATNASKPLEIWWDTVD-SSQYYVFIHFAEVVELRGNQSRGFNI 302

Query: 302 NLNGQHLFGPLSPIYLSTRTFYSETALYDPDGKYVVSLASTENSTLPPLINAFEVYKVIY 361
             NG H +GPL P YLST T YS   L D   ++  SL   EN+TLPP+INAFE+Y V  
Sbjct: 303 MHNGDHFYGPLIPSYLSTLTIYSLEPL-DAADRHTFSLIPIENATLPPIINAFEIYTVKD 362

Query: 362 LSDLQTDPAD-------------------------------------------------- 421
           +S+L +D  D                                                  
Sbjct: 363 ISELASDKGDVDAITNIKSTYGVKKDWQGDPCVPMRYPWSGLNCSNEAAPRITSLNLSTS 422

Query: 422 ---------------------------GKVPDFLVNMSSLKVIKLDKNNLTSPLPPELLK 481
                                      GKVPDFL ++  LK++ L  NNL+ P+P +LLK
Sbjct: 423 GLNGEISSYISNLTMLQTLDLSNNNLTGKVPDFLSSLLHLKLLNLGNNNLSGPIPADLLK 482

Query: 482 RVNNDSLTLSVEDNPNLDSACASNSC-SEEKKKSFVVPVVAPIGGLLVILTVAAVIFWII 541
           R N+ SL+LSV  N NLD  C S+ C   EKK + ++P+VA IGG+LVI T+AA+ FWII
Sbjct: 483 RSNDGSLSLSVGGNQNLD-GCTSDPCPKNEKKNNIIIPIVASIGGVLVIATIAAITFWII 542

Query: 542 ISWNKPHGQTTVPVPRVKDT--ECGNDSLEVRRRQFTFTEVMRMTDNFARPLGEGGSGKV 601
            S  KP G+  V V    +T  + GN SLEVRR+QFT++EV++MT+NF R LG+GG G+V
Sbjct: 543 KSRKKPQGKNVVSVVDKSETNSQFGN-SLEVRRQQFTYSEVVKMTNNFTRVLGKGGFGEV 602

Query: 602 YLGHIDNVQVAVKMLSPLSAHSYQQFQTEVKLLMRVYHGNLTSLVGYLNEKNHFGLIYEY 661
           Y G ID++QVAVKMLS  S+  Y+QFQ EV LLMRV+H NLTSLVGYLNE NH GLIYEY
Sbjct: 603 YYGVIDDIQVAVKMLSLSSSQGYRQFQAEVTLLMRVHHRNLTSLVGYLNEGNHLGLIYEY 662

Query: 662 MTKGNLPQVLSE-DAVLLTRENRLQIAIGAAQGLEYLRHGCKPPIVHRDVKTTNILLTDD 721
           M  G+L + LSE    +L  E+RL+IA+ AAQGLEYL +GCKPPIVHRDVKTTNILLTD+
Sbjct: 663 MANGDLAEQLSERSPSILNWEDRLRIAMDAAQGLEYLHYGCKPPIVHRDVKTTNILLTDN 722

Query: 722 FQAKLADFGLSKSFPKDDSKTHMSTMVAGTPDYFDPEKRSHNSMGDR------------- 740
           FQ KLADFGLSKSFP  D  THMST+VAGTP Y DPE    N + ++             
Sbjct: 723 FQGKLADFGLSKSFP-IDGNTHMSTVVAGTPGYLDPEYYVSNRLTEKSDVYSFGIALLEI 782

BLAST of Lag0003075 vs. ExPASy TrEMBL
Match: A0A1S3CHD1 (probable LRR receptor-like serine/threonine-protein kinase At1g51860 OS=Cucumis melo OX=3656 GN=LOC103500756 PE=4 SV=1)

HSP 1 Score: 691.8 bits (1784), Expect = 3.1e-195
Identity = 412/895 (46.03%), Postives = 539/895 (60.22%), Query Frame = 0

Query: 1   MIMEILRSAFGFLGCLAALQVLVQAQVQSSFISLDCGLPANSSYEESTTGINYISDAAYI 60
           M + +L S FG L C   L +LVQAQ QS FISLDCG P  +SY E+TT I+Y+SDA YI
Sbjct: 4   MELSVLHSLFGLLLCSLTLLLLVQAQDQSGFISLDCGTPEGTSYTETTTKIDYVSDAPYI 63

Query: 61  NT------------------VKVKIYPPNTEIYINDRCG-------RFGFLYGNYDGLDR 120
           N+                   K++ +P       N           R GFLYGNYD L+ 
Sbjct: 64  NSGVSRSVASVYGDTYQQQMRKLRSFPQGIRNCYNVSVKKDTKYLIRAGFLYGNYDALNS 123

Query: 121 LPIFNLYVGQSVWDSVEFTEVDIDVWKEIIHIPLTDNIYICLINTNQGTPFISALEFRPL 180
           LP+F+LY+G S+W+++ FT+V IDV+K++IHI  ++ ++ICLINT  G PFISALEFRPL
Sbjct: 124 LPMFDLYIGNSLWETINFTKVGIDVFKDLIHITSSNKVHICLINTGNGVPFISALEFRPL 183

Query: 181 PNSTYTTTAGSLSLVSRANFGT--DRTYRFGYDVFDRIWTPFTYHEWTNISTSNTVDLTN 240
            N TY T AGSLSL  R + G+  +RTYRF +DV+DR+W+PF ++ WT +ST+ TVD T 
Sbjct: 184 LNVTYQTAAGSLSLDYRLDVGSTENRTYRFPFDVYDRMWSPFNFNAWTQVSTNRTVDETG 243

Query: 241 HDIYEVPSIVMSTAATPTNSSSSLEFKWNSDEVTPQYYAYMYSAEIVKLQANQTRVFNVN 300
           H+ ++ PS+VM TA+   N+S  LE  W++++ + QYY +++ AE+V+LQ NQ+R FN+ 
Sbjct: 244 HNNHQPPSVVMQTASARINASEPLEIWWDTED-SSQYYVFIHVAEVVELQGNQSRGFNIM 303

Query: 301 LNGQHLFGPLSPIYLSTRTFYSETALYDPDGKYVVSLASTENSTLPPLINAFEVYKVIYL 360
            NG   +GPL P YLST T +    L D   ++  S    EN+TLPP++NAFEVY V  +
Sbjct: 304 HNGDLFYGPLIPSYLSTLTIFGHEPL-DAADRHTFSFTPIENATLPPIVNAFEVYIVKDI 363

Query: 361 SDLQTDPAD--------------------------------------------------- 420
           S+L+ D  D                                                   
Sbjct: 364 SELEADKGDVEAITNIKSTYGVKKDWQGDPCVPMGYPWSGLNCSNEAEPRIISLNLSASG 423

Query: 421 --------------------------GKVPDFLVNMSSLKVIKLDKNNLTSPLPPELLKR 480
                                     G VPDFL ++S LK + L  N L+ P+P ELLKR
Sbjct: 424 LNGEISSYISSLTMLQTLDLSNNNLTGHVPDFLSSLSHLKTLNLGNNKLSGPIPAELLKR 483

Query: 481 VNNDSLTLSVEDNPNLDSACASNSC--SEEKKKSFVVPVVAPIGGLLVILTVAAVIFWII 540
            N+ SL+LSV  N NL+  CAS+ C  +EEKK + ++P+VA IGG LV++ + A+ FWII
Sbjct: 484 SNDGSLSLSVGGNQNLE-GCASDPCPKNEEKKNNIIIPIVASIGGFLVVVAIVAITFWII 543

Query: 541 ISWNKPHGQTTVPVPRVKDTECG-NDSLEVRRRQFTFTEVMRMTDNFARPLGEGGSGKVY 600
            S  K  G+  V V    +T      SLEVR RQFT++EV++MT+NF + LG+GG G+VY
Sbjct: 544 KSRKKQQGKNVVSVVDKSETNSQLGTSLEVRSRQFTYSEVVKMTNNFKKVLGKGGFGEVY 603

Query: 601 LGHIDNVQVAVKMLSPLSAHSYQQFQTEVKLLMRVYHGNLTSLVGYLNEKNHFGLIYEYM 660
            G ID V+VAVKMLS  S+  Y+QFQ EV LLMRV+H NLTSLVGYLNE+NH GLIYEYM
Sbjct: 604 YGVIDEVEVAVKMLSLSSSQGYRQFQAEVTLLMRVHHRNLTSLVGYLNEENHIGLIYEYM 663

Query: 661 TKGNLPQVLSEDAV-LLTRENRLQIAIGAAQGLEYLRHGCKPPIVHRDVKTTNILLTDDF 720
             G+L + LSE ++ +++ E+RL+IA+ AAQGLEYL +GCKPPIVHRDVKTTNILLTD+F
Sbjct: 664 ANGDLAEHLSERSLRIISWEDRLRIAMDAAQGLEYLHYGCKPPIVHRDVKTTNILLTDNF 723

Query: 721 QAKLADFGLSKSFPKDDSKTHMSTMVAGTPDYFDPEKRSHNSM----------------- 751
           Q KLADFGLSKSFP  D  THMST+VAGTP Y DPE    N +                 
Sbjct: 724 QGKLADFGLSKSFP-TDGNTHMSTVVAGTPGYLDPEYYVSNRLTEKSDVYSFGIALLEII 783

BLAST of Lag0003075 vs. ExPASy TrEMBL
Match: A0A5D3BYB9 (Putative LRR receptor-like serine/threonine-protein kinase OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold347G00560 PE=4 SV=1)

HSP 1 Score: 688.0 bits (1774), Expect = 4.5e-194
Identity = 411/899 (45.72%), Postives = 538/899 (59.84%), Query Frame = 0

Query: 1   MIMEILRSAFGFLGCLAALQVLVQAQVQSSFISLDCGLPANSSYEESTTGINYISDAAYI 60
           M + +L S FG L C   L +LVQAQ QS FISLDCG P  +SY E+TT I+Y+SDA YI
Sbjct: 4   MELSVLHSLFGLLLCSLTLLLLVQAQDQSGFISLDCGTPEGTSYTETTTKIDYVSDAPYI 63

Query: 61  NT------------------VKVKIYPPNTEIYINDRCG-------RFGFLYGNYDGLDR 120
           N+                   K++ +P       N           R GFLYGNYD L+ 
Sbjct: 64  NSGVSRSVASVYGDTYQQQMRKLRSFPQGIRNCYNVSVKKDTKYLIRAGFLYGNYDALNS 123

Query: 121 LPIFNLYVGQSVWDSVEFTEVDIDVWKEIIHIPLTDNIYICLINTNQGTPFISALEFRPL 180
           LP+F+LY+G S+W+++ FT+V IDV+K++IHI  ++ ++ICLINT  G PFISALEFRPL
Sbjct: 124 LPMFDLYIGNSLWETINFTKVGIDVFKDLIHITSSNKVHICLINTGNGVPFISALEFRPL 183

Query: 181 PNSTYTTTAGSLSLVSRANFGT--DRTYRFGYDVFDRIWTPFTYHEWTNISTSNTVDLTN 240
            N TY T AGSLSL  R + G+  +RTYRF +DV+DR+W+PF ++ WT +ST+ TVD T 
Sbjct: 184 LNVTYQTAAGSLSLDYRLDVGSTENRTYRFPFDVYDRMWSPFNFNAWTQVSTNRTVDETG 243

Query: 241 HDIYEVPSIVMSTAATPTNSSSSLEFKWNSDEVTPQYYAYMYSAEIVKLQANQTRVFNVN 300
           H+ ++ PS+VM TA+   N+S  LE  W++++ + QYY +++ AE+V+LQ NQ+R FN+ 
Sbjct: 244 HNNHQPPSVVMQTASARINASEPLEIWWDTED-SSQYYVFIHVAEVVELQGNQSRGFNIM 303

Query: 301 LNGQHLFGPLSPIYLSTRTFYSETALYDPDGKYVVSLASTENSTLPPLINAFEVYKVIYL 360
            NG   +GPL P YLST T +    L D   ++  S    EN+TLPP++NAFEVY V  +
Sbjct: 304 HNGDLFYGPLIPSYLSTLTIFGHEPL-DAADRHTFSFTPIENATLPPIVNAFEVYIVKDI 363

Query: 361 SDLQTDPAD--------------------------------------------------- 420
           S+L+ D  D                                                   
Sbjct: 364 SELEADKGDVEAITNIKSTYGVKKDWQGDPCVPMGYPWSGLNCSNEAEPRIISLNLSASG 423

Query: 421 --------------------------GKVPDFLVNMSSLKVIKLDKNNLTSPLPPELLKR 480
                                     G VPDFL ++S LK + L  N L+ P+P ELLKR
Sbjct: 424 LNGEISSYISSLTMLQTLDLSNNNLTGHVPDFLSSLSHLKTLNLGNNKLSGPIPAELLKR 483

Query: 481 VNNDSLTLSVEDNPNLDSACASNSC--SEEKKKSFVVPVVAPIGGLLVILTVAAVIFWII 540
            N+ SL+LSV  N NL+  CAS+ C  +EEKK + ++P+VA IGG LV++ + A+ FWII
Sbjct: 484 SNDGSLSLSVGGNQNLE-GCASDPCPKNEEKKNNIIIPIVASIGGFLVVVAIVAITFWII 543

Query: 541 ISWNKPHGQTTVPVPRVKDTECG-NDSLEVRRRQFTFTEVMRMTDNFARPLGEGGSGKVY 600
            S  K  G+  V V    +T      SLEVR RQFT++EV++MT+NF + LG+GG G+VY
Sbjct: 544 KSRKKQQGKNVVSVVDKSETNSQLGTSLEVRSRQFTYSEVVKMTNNFKKVLGKGGFGEVY 603

Query: 601 LGHIDNVQVAVKMLSPLSAHSYQQFQTEVKLLMRVYHGNLTSLVGYLNEKNHFGLIYEYM 660
            G ID V+VAVKMLS  S+  Y+QFQ EV LLMRV+H NLTSLVGYLNE+NH GLIYEYM
Sbjct: 604 YGVIDEVEVAVKMLSLSSSQGYRQFQAEVTLLMRVHHRNLTSLVGYLNEENHIGLIYEYM 663

Query: 661 TKGNLPQVLS-----EDAVLLTRENRLQIAIGAAQGLEYLRHGCKPPIVHRDVKTTNILL 720
             G+L + LS     ++  +++ E+RL+IA+ AAQGLEYL +GCKPPIVHRDVKTTNILL
Sbjct: 664 ANGDLAEHLSGKSNGDNLRIISWEDRLRIAMDAAQGLEYLHYGCKPPIVHRDVKTTNILL 723

Query: 721 TDDFQAKLADFGLSKSFPKDDSKTHMSTMVAGTPDYFDPEKRSHNSM------------- 751
           TD+FQ KLADFGLSKSFP  D  THMST+VAGTP Y DPE    N +             
Sbjct: 724 TDNFQGKLADFGLSKSFP-TDGNTHMSTVVAGTPGYLDPEYYVSNRLTEKSDVYSFGIAL 783

BLAST of Lag0003075 vs. ExPASy TrEMBL
Match: A0A6J1CSK1 (LRR receptor-like serine/threonine-protein kinase IOS1 OS=Momordica charantia OX=3673 GN=LOC111013830 PE=4 SV=1)

HSP 1 Score: 682.9 bits (1761), Expect = 1.5e-192
Identity = 406/890 (45.62%), Postives = 543/890 (61.01%), Query Frame = 0

Query: 7   RSAFGFLGCLAALQVLVQAQVQSSFISLDCGLPANSSYEESTTGINYISDAAYINT---- 66
           +S F FL    AL +LVQAQ QS FISLDCG P  ++Y E+TT INY+SDA+YIN+    
Sbjct: 6   QSLFCFLLSGLALLLLVQAQDQSGFISLDCGTPEGTTYTETTTNINYVSDASYINSGVSG 65

Query: 67  --------------VKVKIYPPNTEIYINDRCG-------RFGFLYGNYDGLDRLPIFNL 126
                          K++ +P       N           R  FLYGNYDGL+ LP F+L
Sbjct: 66  SVAPIYGDTFQRQMRKLRSFPQGIRNCYNVSIKRGTKYLIRANFLYGNYDGLNLLPKFDL 125

Query: 127 YVGQSVWDSVEFTEVDIDVWKEIIHIPLTDNIYICLINTNQGTPFISALEFRPLPNSTYT 186
           Y+G S+W++V FT+VDID +K++IH   ++ +++CLINT  G PFISALEFRPL N TY 
Sbjct: 126 YIGNSLWETVNFTKVDIDTFKDLIHTTSSNEVHVCLINTGDGVPFISALEFRPLLNVTYQ 185

Query: 187 TTAGSLSLVSRANFGT--DRTYRFGYDVFDRIWTPFTYHEWTNISTSNTVDLTNHDIYEV 246
           T +GSLSL  R + G+  + +YRF YDV+DRIW+PF ++EWT +ST+ TVD T+H+ ++ 
Sbjct: 186 TASGSLSLNFRLDIGSTENTSYRFPYDVYDRIWSPFNFNEWTQVSTNQTVDATDHNNHQP 245

Query: 247 PSIVMSTAATPTNSSSSLEFKWNSDEVTPQYYAYMYSAEIVKLQANQTRVFNVNLNGQHL 306
           PSIVM TA+TP N+S  LE  W++ + T +YY +++ AE+ +LQ NQ+R F++  NG+  
Sbjct: 246 PSIVMKTASTPINASKPLEIWWDAVDST-RYYVFIHVAEVGELQPNQSRGFDIMHNGELF 305

Query: 307 FGPLSPIYLSTRTFYSETALYDPDGKYVVSLASTENSTLPPLINAFEVYKVIYLSDLQTD 366
           +GPL P YLST T + +  L D   +++ S    +N+TLPP++NAFE+Y    +S+L+ D
Sbjct: 306 YGPLIPSYLSTLTIFGQKPL-DAANRHLFSFIPIKNATLPPIVNAFEIYTAKDISELEAD 365

Query: 367 PAD--------------------------------------------------------- 426
             D                                                         
Sbjct: 366 KGDVDAITNINSTYGVKRDWQGDPCVPMGYPWSGINCSNEATPRIISLNLSASGLTGEIS 425

Query: 427 --------------------GKVPDFLVNMSSLKVIKLDKNNLTSPLPPELLKRVNNDSL 486
                               GKVPDFL  +S LK + L  N L+ P+P +LLKR N+ SL
Sbjct: 426 SYISNLTMLQTLDLSNNNLTGKVPDFLSTLSHLKTLNLGNNKLSGPIPADLLKRSNDGSL 485

Query: 487 TLSVEDNPNLDSACASNSCS--EEKKKSFVVPVVAPIGGLLVILTVAAVIFWIIISWNKP 546
           +LSV  N NLD  CA +SCS  EEK K+ ++P+VA IGG LV+L ++A+ FW II   + 
Sbjct: 486 SLSVGGNQNLD-GCALDSCSKNEEKNKNVIIPIVASIGGFLVVLAISAITFW-IIKCRRK 545

Query: 547 HGQTTVPVPRVKDT--ECGNDSLEVRRRQFTFTEVMRMTDNFARPLGEGGSGKVYLGHID 606
            G+  + V    +T  + GN SLEVRRRQFT++EV++MT+NF R LG+GG G+VY G +D
Sbjct: 546 QGKNVISVVEKSETNSQFGN-SLEVRRRQFTYSEVVKMTNNFKRVLGKGGFGEVYHGVVD 605

Query: 607 NVQVAVKMLSPLSAHSYQQFQTEVKLLMRVYHGNLTSLVGYLNEKNHFGLIYEYMTKGNL 666
           +VQVAVKMLS  S+  Y++FQ EV LLMRV+H NLTSLVGYL+E NH GLIYEYM  G+L
Sbjct: 606 DVQVAVKMLSLSSSQGYREFQAEVTLLMRVHHRNLTSLVGYLSEGNHLGLIYEYMANGDL 665

Query: 667 PQVLSEDA-VLLTRENRLQIAIGAAQGLEYLRHGCKPPIVHRDVKTTNILLTDDFQAKLA 726
            + LSE +  +L+ E+RL+IA+ AAQGLEYL +GCKPPIVHRDVKTTNILLT++ Q KLA
Sbjct: 666 AEHLSERSNRILSWEDRLRIAMDAAQGLEYLHYGCKPPIVHRDVKTTNILLTENLQGKLA 725

Query: 727 DFGLSKSFPKDDSKTHMSTMVAGTPDYFDPEKRSHNSM---------------------- 751
           DFGLSKSFP  D KTH+ST+VAGTP Y DPE    N +                      
Sbjct: 726 DFGLSKSFP-IDGKTHVSTVVAGTPGYLDPEYYVSNRLTEKSDVYSFGIALLEIISCRPV 785

BLAST of Lag0003075 vs. TAIR 10
Match: AT1G51800.1 (Leucine-rich repeat protein kinase family protein )

HSP 1 Score: 486.9 bits (1252), Expect = 3.0e-137
Identity = 334/894 (37.36%), Postives = 462/894 (51.68%), Query Frame = 0

Query: 9   AFGFLGCLAALQV-----LVQAQVQSSFISLDCGLPANSSYEESTTGINYISDAAYIN-- 68
           AF     L  LQ+     L  AQ QS FISLDCG P  +S+ E TT I YISDA +IN  
Sbjct: 2   AFSSCFLLVLLQIFSALLLCLAQDQSGFISLDCGSPRETSFREKTTNITYISDANFINTG 61

Query: 69  ----------------TVKVKIYPP------NTEIYINDR-CGRFGFLYGNYDGLDRLPI 128
                           T  ++ +P          + I D    R  FL+G YD       
Sbjct: 62  VGGSIKQGYRTQFQQQTWNLRSFPQGIRNCYTLNLTIGDEYLIRANFLHGGYDDKPSTQ- 121

Query: 129 FNLYVGQSVWDSVEFT-EVDIDVWKEIIHIPLTDNIYICLINTNQGTPFISALEFRPLPN 188
           F LY+G ++W +V  T E +  ++ E+IHI  TD + ICL+ T   TPFISALE R L N
Sbjct: 122 FELYLGPNLWSTVTTTNETEASIF-EMIHILTTDRLQICLVKTGNATPFISALELRKLMN 181

Query: 189 STYTTTAGSLSLVSRANFG--TDRTYRFGYDVFDRIWTPFTYHEWTNISTSNTVDLTNHD 248
           +TY T  GSL    RA+ G   ++ YR+G DVFDR+WTP+ +  W+ IST+ +V++ N  
Sbjct: 182 TTYLTRQGSLQTFIRADVGATVNQGYRYGIDVFDRVWTPYNFGNWSQISTNQSVNINND- 241

Query: 249 IYEVPSIVMSTAATPTNSSSSLEFKWNSDEVTPQYYAYMYSAEIVKLQANQTRVFNVNLN 308
            Y+ P I M TA+ PT+  +++       E T Q+Y +M+ AEI +L++N TR FN+  N
Sbjct: 242 -YQPPEIAMVTASVPTDPDAAMNISLVGVERTVQFYVFMHFAEIQELKSNDTREFNIMYN 301

Query: 309 GQHLFGPLSPIYLSTRTFYSET-ALYDPDGKYVVSLASTENSTLPPLINAFEVYKVIYLS 368
            +H++GP  P+  +T + ++ T  + D +G+Y+ SL  T NSTLPPL+NA E+Y V  L 
Sbjct: 302 NKHIYGPFRPLNFTTSSVFTPTEVVADANGQYIFSLQRTGNSTLPPLLNAMEIYSVNLLP 361

Query: 369 DLQTD---------------------------PAD------------------------- 428
             +TD                           P D                         
Sbjct: 362 QQETDRKEVDAMMNIKSAYGVNKIDWEGDPCVPLDYKWSGVNCTYVDNETPKIISLDLST 421

Query: 429 ----------------------------GKVPDFLVNMSSLKVIKLDKNNLTSPLPPELL 488
                                       G VP+FL NM +LK+I L  N L   +P  LL
Sbjct: 422 SGLTGEILEFISDLTSLEVLDLSNNSLTGSVPEFLANMETLKLINLSGNELNGSIPATLL 481

Query: 489 KRVNNDSLTLSVEDNPNLDSACASNSCSEEKKKSFVVPVVAPIGGLL--VILTVAAVIFW 548
            +    S+TLS+E N  L   C+S SC+  KKK     V+AP+   L  V L  A ++ +
Sbjct: 482 DKERRGSITLSIEGNTGL---CSSTSCATTKKKK-KNTVIAPVAASLVSVFLIGAGIVTF 541

Query: 549 IIISWNK-------PHGQTTVPVPRVKDTECGNDSLEVRRRQFTFTEVMRMTDNFARPLG 608
           +I+   K       P+  T       +        +  + R+ T+ +V+++T+NF R LG
Sbjct: 542 LILKRKKRTKLGLNPNSGTGTTPLHSRSHHGFEPPVIAKNRKLTYIDVVKITNNFERVLG 601

Query: 609 EGGSGKVYLGHIDNVQVAVKMLSPLSAHSYQQFQTEVKLLMRVYHGNLTSLVGYLNEKNH 668
            GG G VY G ++N  VAVKML+  +A  Y+QF+ EV+LL+RV+H +LT LVGY  E + 
Sbjct: 602 RGGFGVVYYGVLNNEPVAVKMLTESTALGYKQFKAEVELLLRVHHKDLTCLVGYCEEGDK 661

Query: 669 FGLIYEYMTKGNLPQVLS--EDAVLLTRENRLQIAIGAAQGLEYLRHGCKPPIVHRDVKT 728
             LIYE+M  G+L + LS      +LT E RL+IA  +AQGLEYL +GCKP IVHRD+KT
Sbjct: 662 MSLIYEFMANGDLKEHLSGKRGPSILTWEGRLRIAAESAQGLEYLHNGCKPQIVHRDIKT 721

Query: 729 TNILLTDDFQAKLADFGLSKSFPKDDSKTHMSTMVAGTPDYFDPE--------------- 742
           TNILL + FQAKLADFGLS+SFP   ++TH+ST+VAGTP Y DPE               
Sbjct: 722 TNILLNEKFQAKLADFGLSRSFPL-GTETHVSTIVAGTPGYLDPEYYRTNWLTEKSDVFS 781

BLAST of Lag0003075 vs. TAIR 10
Match: AT1G51890.2 (Leucine-rich repeat protein kinase family protein )

HSP 1 Score: 477.6 bits (1228), Expect = 1.8e-134
Identity = 316/830 (38.07%), Postives = 449/830 (54.10%), Query Frame = 0

Query: 12  FLGCLAALQVLVQAQVQSSFISLDCGL-PANSSYEESTTGINYISDAAYINT-VKVKIYP 71
           FL  + A+  LVQAQ QS FISLDCGL P   +Y E +T I Y SDA YI++ V  KI  
Sbjct: 6   FLIFVFAVLGLVQAQDQSGFISLDCGLVPTEITYVEKSTNITYRSDATYIDSGVPGKI-- 65

Query: 72  PNTEIYIND----------------RCGRFG------------FLYGNYDGLDRLPIFNL 131
              E+Y                    C  F             F+YGNYDGL++LP F+L
Sbjct: 66  --NEVYRTQFQQQIWALRSFPEGQRNCYNFSLTAKRKYLIRGTFIYGNYDGLNQLPSFDL 125

Query: 132 YVGQSVWDSVEFTEVDIDVWKEIIHIPLTDNIYICLINTNQGTPFISALEFRPLPNSTYT 191
           Y+G + W SV    V      E+IH+   D++ ICL+ T + TPFIS+LE RPL N+TY 
Sbjct: 126 YIGPNKWTSVSIPGVRNGSVSEMIHVLRQDHLQICLVKTGETTPFISSLELRPLNNNTYV 185

Query: 192 TTAGSLSLVSRANFG-TDRTYRFGYDVFDRIWTPFTYHEWTNISTSNTVDLTNHDIYEVP 251
           T +GSL +V+R  F  T    R+  DV DRIW PF  ++ + +ST  +VD +N   Y VP
Sbjct: 186 TKSGSLIVVARLYFSPTPPFLRYDEDVHDRIWIPFLDNKNSLLSTELSVDTSN--FYNVP 245

Query: 252 SIVMSTAATPTNSSSSLEFKWNSDEVTPQYYAYMYSAEIVKLQANQTRVFNVNLN-GQHL 311
             V  TAA P N++  L+  W+ D++T Q Y YM+ AEI  L+AN+TR FN+  N G++ 
Sbjct: 246 QTVAKTAAVPLNATQPLKINWSLDDITSQSYIYMHFAEIENLEANETREFNITYNGGENW 305

Query: 312 FGPLSPIYLSTRTFYSETALYDPDGKYVVSLASTENSTLPPLINAFEVYKVIYLSDLQT- 371
           F    P      T Y+  A+   DG +  + + T NST PPLIN  E+Y+V+ L  L T 
Sbjct: 306 FSYFRPPKFRITTVYNPAAVSSLDGNFNFTFSMTGNSTHPPLINGLEIYQVLELPQLDTY 365

Query: 372 ------------------------DPADGKVPDF------LVNMSSLKVIKLD---KNNL 431
                                   DP   ++  +        N +  ++I L+     NL
Sbjct: 366 QDEVSAMMNIKTIYGLSKRSSWQGDPCAPELYRWEGLNCSYPNFAPPQIISLNLSGNKNL 425

Query: 432 TSPLPPELLKRVNNDSLTLSVEDNPNLDSACASNSCSEEKKKSFVVPVVAPIGGLLVILT 491
              +P  L KR++N SLTL  ++                K  + VV + A +  +  +L 
Sbjct: 426 NRSVPETLQKRIDNKSLTLIRDET--------------GKNSTNVVAIAASVASVFAVLV 485

Query: 492 VAAVIFWIIIS---WNKPHGQTTVPVPRVK-DTECGNDSLEVRRRQFTFTEVMRMTDNFA 551
           + A++F +I      N+  G  +     VK D    + S+  + R+FT++EV++MT NF 
Sbjct: 486 ILAIVFVVIRKKQRTNEASGPRSFTTGTVKSDARSSSSSIITKERKFTYSEVLKMTKNFE 545

Query: 552 RPLGEGGSGKVYLGHIDNVQVAVKMLSPLSAHSYQQFQTEVKLLMRVYHGNLTSLVGYLN 611
           R LG+GG G VY G++D+ QVAVKMLS  SA  Y++F+ EV+LL+RV+H +L  LVGY +
Sbjct: 546 RVLGKGGFGTVYHGNLDDTQVAVKMLSHSSAQGYKEFKAEVELLLRVHHRHLVGLVGYCD 605

Query: 612 EKNHFGLIYEYMTKGNLPQVLS--EDAVLLTRENRLQIAIGAAQGLEYLRHGCKPPIVHR 671
           + ++  LIYEYM KG+L + +S      +L+ E R+QIA+ AAQGLEYL +GC+PP+VHR
Sbjct: 606 DGDNLALIYEYMEKGDLRENMSGKHSVNVLSWETRMQIAVEAAQGLEYLHNGCRPPMVHR 665

Query: 672 DVKTTNILLTDDFQAKLADFGLSKSFPKDDSKTHMSTMVAGTPDYFDPEKRSHNSMGDR- 731
           DVK TNILL +  QAKLADFGLS+SFP  D ++H+ T+VAGTP Y DPE    N + ++ 
Sbjct: 666 DVKPTNILLNERSQAKLADFGLSRSFPV-DGESHVMTVVAGTPGYLDPEYYRTNWLSEKS 725

Query: 732 ---------------HYCARGHQERDR-------------------PRLKGEFDSNSAWK 735
                                ++ER                     P+L  ++D+N  WK
Sbjct: 726 DVYSFGVVLLEIVTNQPVMNKNRERPHINEWVMFMLTNGDIKSIVDPKLNEDYDTNGVWK 785

BLAST of Lag0003075 vs. TAIR 10
Match: AT4G29990.1 (Leucine-rich repeat transmembrane protein kinase protein )

HSP 1 Score: 471.1 bits (1211), Expect = 1.7e-132
Identity = 314/862 (36.43%), Postives = 443/862 (51.39%), Query Frame = 0

Query: 22  LVQAQVQSSFISLDCGLPANSSYEESTTGINYISDAAYINT------------------- 81
           LV AQ QS FIS+DCG+P +SSY +  T + Y+SD  ++ +                   
Sbjct: 18  LVFAQDQSGFISIDCGIPDDSSYTDEKTNMKYVSDLGFVESGTSHSIVSDLQTTSLERQF 77

Query: 82  VKVKIYPPNTEIYINDR---------CGRFGFLYGNYDGLDRLPIFNLYVGQSVWDSVEF 141
             V+ +P       + R           R  F+YGNYDG  + P F+LY+G ++W+SV  
Sbjct: 78  QNVRSFPEGKRNCYDIRPQQGKGFKYLIRTRFMYGNYDGFSKTPEFDLYIGANLWESVVL 137

Query: 142 TEVDIDVWKEIIHIPLTDNIYICLINTNQGTPFISALEFRPLPNSTYTTTAGSLSLVSRA 201
                 + KEII+ P +D+I++CL++ N+GTPF+S LE R L N TY T   +L L  R 
Sbjct: 138 INETAIMTKEIIYTPPSDHIHVCLVDKNRGTPFLSVLEIRFLKNDTYDTPYEALMLGRRW 197

Query: 202 NFGTDRTYRFGY--DVFDRIWTPFTYHEWTNISTSNTVDLTNHDIYEVPSIVMSTAATPT 261
           +FGT    +  Y  D +DRIW P+       ++TS T+D TNH+ +   SIVM +A  P 
Sbjct: 198 DFGTATNLQIRYKDDFYDRIWMPYKSPYQKTLNTSLTIDETNHNGFRPASIVMRSAIAPG 257

Query: 262 NSSSSLEFKWNSDEVTPQYYAYMYSAEIVKLQANQTRVFNVNLNGQHLFGPLSPIYLSTR 321
           N S+ L+F W  D+   ++Y YM+ AE+ +LQ N+TR F++ +N   L     P YL T 
Sbjct: 258 NESNPLKFNWAPDDPRSKFYIYMHFAEVRELQRNETREFDIYINDVILAENFRPFYLFTD 317

Query: 322 TFYSETALYDPDGKYV--VSLASTENSTLPPLINAFEVYKVIYLSDLQTDPAD------- 381
           T     +  DP G+ +  + L  T  STLPP+INA E+Y++     L TD  D       
Sbjct: 318 T----RSTVDPVGRKMNEIVLQRTGVSTLPPIINAIEIYQINEFLQLPTDQQDVDAMTKI 377

Query: 382 ------------------------------------------------------------ 441
                                                                       
Sbjct: 378 KFKYRVKKNWQGDPCVPVDNSWEGLECLHSDNNTSPKSIALNLSSSGLTGQIDPAFANLT 437

Query: 442 -------------GKVPDFLVNMSSLKVIKLDKNNLTSPLPPELLKRVNNDSLTLSVEDN 501
                        GKVPDFL ++ +L  + L+ N LT  +P +LL++  + SL+L    N
Sbjct: 438 SINKLDLSNNSLTGKVPDFLASLPNLTELNLEGNKLTGSIPAKLLEKSKDGSLSLRFGGN 497

Query: 502 PNLDSACASNSC--SEEKKKSFVVPVVAPIGGLLVILTVAAVIFWI-------IISWNKP 561
           P+L   C S SC  + +KK  ++VPVVA + GLL++LT  A+I+          IS NKP
Sbjct: 498 PDL---CQSPSCQTTTKKKIGYIVPVVASLAGLLIVLTALALIWHFKKRSRRGTIS-NKP 557

Query: 562 HGQTTVPVPRVKDTECGNDSLEVRRRQFTFTEVMRMTDNFARPLGEGGSGKVYLGHIDNV 621
            G  T P             L+  +R F ++EV+ +T+NF R LG+GG GKVY G ++  
Sbjct: 558 LGVNTGP-------------LDTAKRYFIYSEVVNITNNFERVLGKGGFGKVYHGFLNGD 617

Query: 622 QVAVKMLSPLSAHSYQQFQTEVKLLMRVYHGNLTSLVGYLNEKNHFGLIYEYMTKGNLPQ 681
           QVAVK+LS  S   Y++F+ EV+LLMRV+H NLTSL+GY NE NH  LIYEYM  GNL  
Sbjct: 618 QVAVKILSEESTQGYKEFRAEVELLMRVHHTNLTSLIGYCNEDNHMALIYEYMANGNLGD 677

Query: 682 VLS-EDAVLLTRENRLQIAIGAAQGLEYLRHGCKPPIVHRDVKTTNILLTDDFQAKLADF 726
            LS + +++L+ E RLQI++ AAQGLEYL +GCKPPIVHRDVK  NILL ++ QAK+ADF
Sbjct: 678 YLSGKSSLILSWEERLQISLDAAQGLEYLHYGCKPPIVHRDVKPANILLNENLQAKIADF 737

BLAST of Lag0003075 vs. TAIR 10
Match: AT1G51860.1 (Leucine-rich repeat protein kinase family protein )

HSP 1 Score: 470.7 bits (1210), Expect = 2.2e-132
Identity = 322/874 (36.84%), Postives = 457/874 (52.29%), Query Frame = 0

Query: 23  VQAQVQSSFISLDCGL-PANSSYEESTTGINYISDAAYINT------------------V 82
           V+AQ Q+ FISLDCGL P  ++Y E +T I Y SD  YI++                   
Sbjct: 21  VEAQNQAGFISLDCGLVPKETTYTEKSTNITYKSDVDYIDSGLVGKINDAYKTQFQQQVW 80

Query: 83  KVKIYPP------NTEIYINDR-CGRFGFLYGNYDGLDRLPIFNLYVGQSVWDSVEFTEV 142
            V+ +P       N  +  N++   R  F+YGNYDGL++ P F+L++G + W SV+   V
Sbjct: 81  AVRSFPVGQRNCYNVNLTANNKYLIRGTFVYGNYDGLNQFPSFDLHIGPNKWSSVKILGV 140

Query: 143 DIDVWKEIIHIPLTDNIYICLINTNQGTPFISALEFRPLPNSTYTTTAGSLSLVSRANFG 202
                 EIIH+   D++ +CL+ T   TPFIS+LE RPL N +Y T +GSL L +R  F 
Sbjct: 141 TNTSMHEIIHVVPQDSLEVCLVKTGPTTPFISSLEVRPLNNESYLTQSGSLMLFARVYFP 200

Query: 203 TDRT--YRFGYDVFDRIWTPFTYHEWTNISTSNTVDLTNHDIYEVPSIVMSTAATPTNSS 262
           +  +   R+  D+ DR+W  FT  E   IST   +D +N   Y++P  VM TAA P N+S
Sbjct: 201 SSSSSFIRYDEDIHDRVWNSFTDDETVWISTDLPIDTSNS--YDMPQSVMKTAAVPKNAS 260

Query: 263 SSLEFKWNSDEVTPQYYAYMYSAEIVKLQANQTRVFNVNLN-GQHLFGPLSPIYLSTRTF 322
                 W  DE T Q Y YM+ AE+  L AN+TR FN+  N G   F  L P  LS  T 
Sbjct: 261 EPWLLWWTLDENTAQSYVYMHFAEVQNLTANETREFNITYNGGLRWFSYLRPPNLSISTI 320

Query: 323 YSETALYDPDGKYVVSLASTENSTLPPLINAFEVYKVIYLSDLQT--------------- 382
           ++  A+   +G +  + A T NSTLPPL+NA E+Y V+ +  L+T               
Sbjct: 321 FNPRAVSSSNGIFNFTFAMTGNSTLPPLLNALEIYTVVDILQLETNKDEVSAMMNIKETY 380

Query: 383 ----------DPA----------------------------------------------- 442
                     DP                                                
Sbjct: 381 GLSKKISWQGDPCAPQLYRWEGLNCSYPDSEGSRIISLNLNGSELTGSITSDISKLTLLT 440

Query: 443 ---------DGKVPDFLVNMSSLKVIKLDKN---NLTSPLPPELLKRVNNDSLTLSVEDN 502
                     G +P F   M SLK+I L  N   NLT+ +P  L +RVN+ SLTL + +N
Sbjct: 441 VLDLSNNDLSGDIPTFFAEMKSLKLINLSGNPNLNLTA-IPDSLQQRVNSKSLTLILGEN 500

Query: 503 PNLDSACASNSCSEEKKKSFVVPVVAPIGGLLVILTVAAVIFWI---IISWNKPHGQTTV 562
             L       +  +E KK  +V + A + G+  +L + A+ F I    +  +K  G   +
Sbjct: 501 LTL-------TPKKESKKVPMVAIAASVAGVFALLVILAIFFVIKRKNVKAHKSPGPPPL 560

Query: 563 PVPRV--KDTECGNDSLEVRRRQFTFTEVMRMTDNFARPLGEGGSGKVYLGHIDNVQVAV 622
             P +   +T   N S+  R R+ T+ EV++MT+NF R LG+GG G VY G++D  +VAV
Sbjct: 561 VTPGIVKSETRSSNPSIITRERKITYPEVLKMTNNFERVLGKGGFGTVYHGNLDGAEVAV 620

Query: 623 KMLSPLSAHSYQQFQTEVKLLMRVYHGNLTSLVGYLNEKNHFGLIYEYMTKGNLPQVLS- 682
           KMLS  SA  Y++F+ EV+LL+RV+H +L  LVGY ++ ++  LIYEYM  G+L + +S 
Sbjct: 621 KMLSHSSAQGYKEFKAEVELLLRVHHRHLVGLVGYCDDGDNLALIYEYMANGDLRENMSG 680

Query: 683 -EDAVLLTRENRLQIAIGAAQGLEYLRHGCKPPIVHRDVKTTNILLTDDFQAKLADFGLS 742
                +LT ENR+QIA+ AAQGLEYL +GC+PP+VHRDVKTTNILL +   AKLADFGLS
Sbjct: 681 KRGGNVLTWENRMQIAVEAAQGLEYLHNGCRPPMVHRDVKTTNILLNERCGAKLADFGLS 740

BLAST of Lag0003075 vs. TAIR 10
Match: AT1G51890.1 (Leucine-rich repeat protein kinase family protein )

HSP 1 Score: 468.8 bits (1205), Expect = 8.3e-132
Identity = 320/878 (36.45%), Postives = 450/878 (51.25%), Query Frame = 0

Query: 12  FLGCLAALQVLVQAQVQSSFISLDCGL-PANSSYEESTTGINYISDAAYINT-VKVKIYP 71
           FL  + A+  LVQAQ QS FISLDCGL P   +Y E +T I Y SDA YI++ V  KI  
Sbjct: 6   FLIFVFAVLGLVQAQDQSGFISLDCGLVPTEITYVEKSTNITYRSDATYIDSGVPGKI-- 65

Query: 72  PNTEIYIND----------------RCGRFG------------FLYGNYDGLDRLPIFNL 131
              E+Y                    C  F             F+YGNYDGL++LP F+L
Sbjct: 66  --NEVYRTQFQQQIWALRSFPEGQRNCYNFSLTAKRKYLIRGTFIYGNYDGLNQLPSFDL 125

Query: 132 YVGQSVWDSVEFTEVDIDVWKEIIHIPLTDNIYICLINTNQGTPFISALEFRPLPNSTYT 191
           Y+G + W SV    V      E+IH+   D++ ICL+ T + TPFIS+LE RPL N+TY 
Sbjct: 126 YIGPNKWTSVSIPGVRNGSVSEMIHVLRQDHLQICLVKTGETTPFISSLELRPLNNNTYV 185

Query: 192 TTAGSLSLVSRANFG-TDRTYRFGYDVFDRIWTPFTYHEWTNISTSNTVDLTNHDIYEVP 251
           T +GSL +V+R  F  T    R+  DV DRIW PF  ++ + +ST  +VD +N   Y VP
Sbjct: 186 TKSGSLIVVARLYFSPTPPFLRYDEDVHDRIWIPFLDNKNSLLSTELSVDTSN--FYNVP 245

Query: 252 SIVMSTAATPTNSSSSLEFKWNSDEVTPQYYAYMYSAEIVKLQANQTRVFNVNLN-GQHL 311
             V  TAA P N++  L+  W+ D++T Q Y YM+ AEI  L+AN+TR FN+  N G++ 
Sbjct: 246 QTVAKTAAVPLNATQPLKINWSLDDITSQSYIYMHFAEIENLEANETREFNITYNGGENW 305

Query: 312 FGPLSPIYLSTRTFYSETALYDPDGKYVVSLASTENSTLPPLINAFEVYKVIYLSDLQT- 371
           F    P      T Y+  A+   DG +  + + T NST PPLIN  E+Y+V+ L  L T 
Sbjct: 306 FSYFRPPKFRITTVYNPAAVSSLDGNFNFTFSMTGNSTHPPLINGLEIYQVLELPQLDTY 365

Query: 372 ------------------------DPA--------------------------------- 431
                                   DP                                  
Sbjct: 366 QDEVSAMMNIKTIYGLSKRSSWQGDPCAPELYRWEGLNCSYPNFAPPQIISLNLSGSNLS 425

Query: 432 -----------------------DGKVPDFLVNMSSLKVIKLDKN-NLTSPLPPELLKRV 491
                                   G +P    +M +L +I L  N NL   +P  L KR+
Sbjct: 426 GTITSDISKLTHLRELDLSNNDLSGDIPFVFSDMKNLTLINLSGNKNLNRSVPETLQKRI 485

Query: 492 NNDSLTLSVEDNPNLDSACASNSCSEEKKKSFVVPVVAPIGGLLVILTVAAVIFWIIIS- 551
           +N SLTL  ++                K  + VV + A +  +  +L + A++F +I   
Sbjct: 486 DNKSLTLIRDET--------------GKNSTNVVAIAASVASVFAVLVILAIVFVVIRKK 545

Query: 552 --WNKPHGQTTVPVPRVK-DTECGNDSLEVRRRQFTFTEVMRMTDNFARPLGEGGSGKVY 611
              N+  G  +     VK D    + S+  + R+FT++EV++MT NF R LG+GG G VY
Sbjct: 546 QRTNEASGPRSFTTGTVKSDARSSSSSIITKERKFTYSEVLKMTKNFERVLGKGGFGTVY 605

Query: 612 LGHIDNVQVAVKMLSPLSAHSYQQFQTEVKLLMRVYHGNLTSLVGYLNEKNHFGLIYEYM 671
            G++D+ QVAVKMLS  SA  Y++F+ EV+LL+RV+H +L  LVGY ++ ++  LIYEYM
Sbjct: 606 HGNLDDTQVAVKMLSHSSAQGYKEFKAEVELLLRVHHRHLVGLVGYCDDGDNLALIYEYM 665

Query: 672 TKGNLPQVLS--EDAVLLTRENRLQIAIGAAQGLEYLRHGCKPPIVHRDVKTTNILLTDD 731
            KG+L + +S      +L+ E R+QIA+ AAQGLEYL +GC+PP+VHRDVK TNILL + 
Sbjct: 666 EKGDLRENMSGKHSVNVLSWETRMQIAVEAAQGLEYLHNGCRPPMVHRDVKPTNILLNER 725

Query: 732 FQAKLADFGLSKSFPKDDSKTHMSTMVAGTPDYFDPEKRSHNSMGDR------------- 735
            QAKLADFGLS+SFP  D ++H+ T+VAGTP Y DPE    N + ++             
Sbjct: 726 SQAKLADFGLSRSFPV-DGESHVMTVVAGTPGYLDPEYYRTNWLSEKSDVYSFGVVLLEI 785