Lag0003018 (gene) Sponge gourd (AG‐4) v1

Overview


Name	Lag0003018
Type	gene
Organism	Luffa acutangula (Sponge gourd (AG‐4) v1)
Description	protein THYLAKOID ASSEMBLY 8, chloroplastic
Location	chr4: 47420837 .. 47421523 (-)
RNA-Seq Expression	Lag0003018
Synteny	Lag0003018

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonCDSpolypeptide

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGGCTTTTTCTCTTCACTCCACATTTCTCAAATCCCAATTTGCAATTCCGATTCCCATCTCCGCCGCAACCGCCGCCGCCGCCGTCTCTTTTCCGGTACGCTGCGGCCCACGCGACAACCGAGGGCCGCTAGTTAAAGGCAGAACCCTAAGCACCGAGGCAATCCAAGCCATTCAATCCCTAAAACGGGCGGAAAGATCCGACCCGACGAAGCTCCAACAAGTCCTCTCAAATACTCTCTCCCGATTGCTCAAAGCCGACCTCGTCGCCGCCCTGAAGGAGCTTCTCCGGCAGGACCGGTGCGCCCTCGCTTTGGAGGTTTTCGCCGTCGTCCGATCGGAGTACGGCGCGGATTTAGGGATGTACGCGGAGGTCGCGGCGGCGCTGTCGAGGAACGGAGCGGCGGAGGAAATGGACCGGCTGGTGTGCGATTTGGACGACGGAGAGAGTAAAATCCAGTGCGATGACAAGGGTTTGATTAAGTTGATCAAGGCGGTGATTGGTGGAGATCGAAGGGAATCGACGGTCAGGATTTATCGGATGATGAGGAGGAGCGGTTGGGGGTCCGCCATTAAAGCTGATGAGTATATGGTTAGGGTTTTGAGTAAGGGTTTAAGGAGACTTGGGGAAGTGGAATTGGCTGATGAGATCAATAGGGAATTTCAAGATTTAGTGGGGACTTTTTGA

mRNA sequence

ATGGCTTTTTCTCTTCACTCCACATTTCTCAAATCCCAATTTGCAATTCCGATTCCCATCTCCGCCGCAACCGCCGCCGCCGCCGTCTCTTTTCCGGTACGCTGCGGCCCACGCGACAACCGAGGGCCGCTAGTTAAAGGCAGAACCCTAAGCACCGAGGCAATCCAAGCCATTCAATCCCTAAAACGGGCGGAAAGATCCGACCCGACGAAGCTCCAACAAGTCCTCTCAAATACTCTCTCCCGATTGCTCAAAGCCGACCTCGTCGCCGCCCTGAAGGAGCTTCTCCGGCAGGACCGGTGCGCCCTCGCTTTGGAGGTTTTCGCCGTCGTCCGATCGGAGTACGGCGCGGATTTAGGGATGTACGCGGAGGTCGCGGCGGCGCTGTCGAGGAACGGAGCGGCGGAGGAAATGGACCGGCTGGTGTGCGATTTGGACGACGGAGAGAGTAAAATCCAGTGCGATGACAAGGGTTTGATTAAGTTGATCAAGGCGGTGATTGGTGGAGATCGAAGGGAATCGACGGTCAGGATTTATCGGATGATGAGGAGGAGCGGTTGGGGGTCCGCCATTAAAGCTGATGAGTATATGGTTAGGGTTTTGAGTAAGGGTTTAAGGAGACTTGGGGAAGTGGAATTGGCTGATGAGATCAATAGGGAATTTCAAGATTTAGTGGGGACTTTTTGA

Coding sequence (CDS)

ATGGCTTTTTCTCTTCACTCCACATTTCTCAAATCCCAATTTGCAATTCCGATTCCCATCTCCGCCGCAACCGCCGCCGCCGCCGTCTCTTTTCCGGTACGCTGCGGCCCACGCGACAACCGAGGGCCGCTAGTTAAAGGCAGAACCCTAAGCACCGAGGCAATCCAAGCCATTCAATCCCTAAAACGGGCGGAAAGATCCGACCCGACGAAGCTCCAACAAGTCCTCTCAAATACTCTCTCCCGATTGCTCAAAGCCGACCTCGTCGCCGCCCTGAAGGAGCTTCTCCGGCAGGACCGGTGCGCCCTCGCTTTGGAGGTTTTCGCCGTCGTCCGATCGGAGTACGGCGCGGATTTAGGGATGTACGCGGAGGTCGCGGCGGCGCTGTCGAGGAACGGAGCGGCGGAGGAAATGGACCGGCTGGTGTGCGATTTGGACGACGGAGAGAGTAAAATCCAGTGCGATGACAAGGGTTTGATTAAGTTGATCAAGGCGGTGATTGGTGGAGATCGAAGGGAATCGACGGTCAGGATTTATCGGATGATGAGGAGGAGCGGTTGGGGGTCCGCCATTAAAGCTGATGAGTATATGGTTAGGGTTTTGAGTAAGGGTTTAAGGAGACTTGGGGAAGTGGAATTGGCTGATGAGATCAATAGGGAATTTCAAGATTTAGTGGGGACTTTTTGA

Protein sequence

MAFSLHSTFLKSQFAIPIPISAATAAAAVSFPVRCGPRDNRGPLVKGRTLSTEAIQAIQSLKRAERSDPTKLQQVLSNTLSRLLKADLVAALKELLRQDRCALALEVFAVVRSEYGADLGMYAEVAAALSRNGAAEEMDRLVCDLDDGESKIQCDDKGLIKLIKAVIGGDRRESTVRIYRMMRRSGWGSAIKADEYMVRVLSKGLRRLGEVELADEINREFQDLVGTF

Homology

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BLAST of Lag0003018 vs. NCBI nr
Match: XP_023522652.1 (protein THYLAKOID ASSEMBLY 8, chloroplastic-like [Cucurbita pepo subsp. pepo] >XP_023548438.1 protein THYLAKOID ASSEMBLY 8, chloroplastic-like [Cucurbita pepo subsp. pepo] >XP_023548440.1 protein THYLAKOID ASSEMBLY 8, chloroplastic-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 382.1 bits (980), Expect = 3.3e-102
Identity = 201/228 (88.16%), Postives = 213/228 (93.42%), Query Frame = 0


Query: 1   MAFSLHSTFLKSQFAIPIPISAATAAAAVSFPVRCGPRDNRGPLVKGRTLSTEAIQAIQS 60
           MAFSLHSTFLKSQ  IPIPIS ATA   VS PVRCGPRDNRGPLVKGRTLSTEAIQAIQS
Sbjct: 1   MAFSLHSTFLKSQIPIPIPIS-ATATVVVSLPVRCGPRDNRGPLVKGRTLSTEAIQAIQS 60

Query: 61  LKRAERSDPTKLQQVLSNTLSRLLKADLVAALKELLRQDRCALALEVFAVVRSEYGADLG 120
           LKRAE+SDPTKL+QVLS TLSRLLKADLVA LKELLRQDRCALALEVFAVVRSEYGADLG
Sbjct: 61  LKRAEKSDPTKLEQVLSTTLSRLLKADLVATLKELLRQDRCALALEVFAVVRSEYGADLG 120

Query: 121 MYAEVAAALSRNGAAEEMDRLVCDLDDGESKIQCDDKGLIKLIKAVIGGDRRESTVRIYR 180
           MYAEVAAALSRNGAAEE+DRLVCDL+D +  IQCDDKGLIKLIKAVIGGDRRESTVRIYR
Sbjct: 121 MYAEVAAALSRNGAAEEIDRLVCDLEDEDRAIQCDDKGLIKLIKAVIGGDRRESTVRIYR 180

Query: 181 MMRRSGWGSAIKADEYMVRVLSKGLRRLGEVELADEINREFQDLVGTF 229
           MM+RSGWGS IKAD+Y VRVLSKGLRRLGE+E+ADE+N +FQDLVG+F
Sbjct: 181 MMKRSGWGSTIKADDYTVRVLSKGLRRLGEMEMADEVNMQFQDLVGSF 227

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BLAST of Lag0003018 vs. NCBI nr
Match: XP_022992386.1 (protein THYLAKOID ASSEMBLY 8, chloroplastic [Cucurbita maxima])

HSP 1 Score: 380.9 bits (977), Expect = 7.5e-102
Identity = 201/228 (88.16%), Postives = 212/228 (92.98%), Query Frame = 0


Query: 1   MAFSLHSTFLKSQFAIPIPISAATAAAAVSFPVRCGPRDNRGPLVKGRTLSTEAIQAIQS 60
           MAFSLHSTFLKSQ  IPIPIS ATA   VS PVRCGPRDNRGPLVKGRTLSTEAIQAIQS
Sbjct: 1   MAFSLHSTFLKSQIPIPIPIS-ATATVVVSLPVRCGPRDNRGPLVKGRTLSTEAIQAIQS 60

Query: 61  LKRAERSDPTKLQQVLSNTLSRLLKADLVAALKELLRQDRCALALEVFAVVRSEYGADLG 120
           LKRAE+SDPTKL+QVLS TLSRLLKADLVA LKELLRQDRCALALEVFAVVRSEYG DLG
Sbjct: 61  LKRAEKSDPTKLEQVLSTTLSRLLKADLVATLKELLRQDRCALALEVFAVVRSEYGVDLG 120

Query: 121 MYAEVAAALSRNGAAEEMDRLVCDLDDGESKIQCDDKGLIKLIKAVIGGDRRESTVRIYR 180
           MYAEVAAALSRNGA EE+DRLVCDL+D +  IQCDDKGLIKLIKAVIGGDRRESTVRIYR
Sbjct: 121 MYAEVAAALSRNGAMEEIDRLVCDLEDEDRVIQCDDKGLIKLIKAVIGGDRRESTVRIYR 180

Query: 181 MMRRSGWGSAIKADEYMVRVLSKGLRRLGEVELADEINREFQDLVGTF 229
           MM+RSGWGS IKAD+YMVRVLSKGLRRLGE+E+ADEIN +FQDLVG+F
Sbjct: 181 MMKRSGWGSTIKADDYMVRVLSKGLRRLGEMEMADEINMQFQDLVGSF 227

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BLAST of Lag0003018 vs. NCBI nr
Match: KAG7014066.1 (Pentatricopeptide repeat-containing protein, partial [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 380.2 bits (975), Expect = 1.3e-101
Identity = 200/228 (87.72%), Postives = 212/228 (92.98%), Query Frame = 0


Query: 1   MAFSLHSTFLKSQFAIPIPISAATAAAAVSFPVRCGPRDNRGPLVKGRTLSTEAIQAIQS 60
           MAFSLHSTFLKSQ  IPIPIS ATA   VS PVRCGPRDNRGPLVKGRTLSTEAIQAIQS
Sbjct: 1   MAFSLHSTFLKSQIPIPIPIS-ATATVVVSLPVRCGPRDNRGPLVKGRTLSTEAIQAIQS 60

Query: 61  LKRAERSDPTKLQQVLSNTLSRLLKADLVAALKELLRQDRCALALEVFAVVRSEYGADLG 120
           LKRAE+SDPTKL+QVLS TLSRLLKADLVA LKELLRQDRCALALEVFAVVRSEYGADLG
Sbjct: 61  LKRAEKSDPTKLEQVLSTTLSRLLKADLVATLKELLRQDRCALALEVFAVVRSEYGADLG 120

Query: 121 MYAEVAAALSRNGAAEEMDRLVCDLDDGESKIQCDDKGLIKLIKAVIGGDRRESTVRIYR 180
           MYAEVAAALSRNGA EE+DRLVCDL+D +  IQCDDKGLIKLIKAVIGGDRRESTVRIYR
Sbjct: 121 MYAEVAAALSRNGAMEEIDRLVCDLEDEDRAIQCDDKGLIKLIKAVIGGDRRESTVRIYR 180

Query: 181 MMRRSGWGSAIKADEYMVRVLSKGLRRLGEVELADEINREFQDLVGTF 229
           MM+RSGWGS IKAD+Y VRVLSKGLRRLGE+E+ADE+N +FQDLVG+F
Sbjct: 181 MMKRSGWGSTIKADDYTVRVLSKGLRRLGEMEMADEVNMQFQDLVGSF 227

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BLAST of Lag0003018 vs. NCBI nr
Match: XP_022954347.1 (protein THYLAKOID ASSEMBLY 8, chloroplastic [Cucurbita moschata])

HSP 1 Score: 379.0 bits (972), Expect = 2.8e-101
Identity = 200/228 (87.72%), Postives = 212/228 (92.98%), Query Frame = 0


Query: 1   MAFSLHSTFLKSQFAIPIPISAATAAAAVSFPVRCGPRDNRGPLVKGRTLSTEAIQAIQS 60
           MAFSLHSTFLKSQ  IPIPISAA A   VS PVRCGPRDNRGPLVKGRTLSTEAIQAIQS
Sbjct: 1   MAFSLHSTFLKSQIPIPIPISAA-ATVVVSLPVRCGPRDNRGPLVKGRTLSTEAIQAIQS 60

Query: 61  LKRAERSDPTKLQQVLSNTLSRLLKADLVAALKELLRQDRCALALEVFAVVRSEYGADLG 120
           LKRAE+SDPTKL+QVLS TLSRLLKADLVA LKELLRQDRCALALEVFAVVRSEYGADLG
Sbjct: 61  LKRAEKSDPTKLEQVLSTTLSRLLKADLVATLKELLRQDRCALALEVFAVVRSEYGADLG 120

Query: 121 MYAEVAAALSRNGAAEEMDRLVCDLDDGESKIQCDDKGLIKLIKAVIGGDRRESTVRIYR 180
           MYAEVAAALSRNGA EE+DRLVCDL+D +  IQCDDKGLIKLIKAVIGGDRRESTVRIYR
Sbjct: 121 MYAEVAAALSRNGAMEEIDRLVCDLEDEDRVIQCDDKGLIKLIKAVIGGDRRESTVRIYR 180

Query: 181 MMRRSGWGSAIKADEYMVRVLSKGLRRLGEVELADEINREFQDLVGTF 229
           MM+RSGWGS IKAD+Y VRVLSKGLRRLGE+E+ADE+N +FQDLVG+F
Sbjct: 181 MMKRSGWGSTIKADDYTVRVLSKGLRRLGEMEMADEVNMQFQDLVGSF 227

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BLAST of Lag0003018 vs. NCBI nr
Match: XP_038898717.1 (protein THYLAKOID ASSEMBLY 8, chloroplastic [Benincasa hispida])

HSP 1 Score: 377.1 bits (967), Expect = 1.1e-100
Identity = 201/226 (88.94%), Postives = 212/226 (93.81%), Query Frame = 0


Query: 1   MAFSLHSTFLKSQFAIPIPISAATAAAAVSFPVRCGPRDNRGPLVKGRTLSTEAIQAIQS 60
           MA S+HSTFLKSQ +IPIP+SAA AA AVS PVRCGPRDNRGPLVKGRTLS EAIQAIQS
Sbjct: 1   MASSIHSTFLKSQISIPIPVSAA-AAVAVSLPVRCGPRDNRGPLVKGRTLSIEAIQAIQS 60

Query: 61  LKRAERSDPTKLQQVLSNTLSRLLKADLVAALKELLRQDRCALALEVFAVVRSEYGADLG 120
           LKRAERSDPTKLQQVLS TLSRLLKADLVA+LKELLRQDRCALALEVFAV+RSEYGADLG
Sbjct: 61  LKRAERSDPTKLQQVLSTTLSRLLKADLVASLKELLRQDRCALALEVFAVIRSEYGADLG 120

Query: 121 MYAEVAAALSRNGAAEEMDRLVCDLDDGESKIQCDDKGLIKLIKAVIGGDRRESTVRIYR 180
           MYAEVAAALSRNGAAEE+DRLVCDLD G+  IQ DDKGLIKLIKAVI GDRRESTVRIYR
Sbjct: 121 MYAEVAAALSRNGAAEEIDRLVCDLDGGDVLIQWDDKGLIKLIKAVISGDRRESTVRIYR 180

Query: 181 MMRRSGWGSAIKADEYMVRVLSKGLRRLGEVELADEINREFQDLVG 227
           MMRR+GWGS IKAD+Y+VRVLSKGLRR GE+ELADEINREFQDLVG
Sbjct: 181 MMRRNGWGSTIKADDYLVRVLSKGLRRFGEMELADEINREFQDLVG 225

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BLAST of Lag0003018 vs. ExPASy Swiss-Prot
Match: Q9LVW6 (Protein THYLAKOID ASSEMBLY 8, chloroplastic OS=Arabidopsis thaliana OX=3702 GN=THA8 PE=2 SV=1)

HSP 1 Score: 186.4 bits (472), Expect = 3.5e-46
Identity = 102/188 (54.26%), Postives = 140/188 (74.47%), Query Frame = 0


Query: 33  VRCGPRDNRGPLVKGRTLSTEAIQAIQSLKRAERSDPTKLQQVLSNTLSRLLKADLVAAL 92
           +RCGPRDNRGPL+KGR LSTEAIQ+IQSLKRA R+  +    +    L RL+K+DL++ L
Sbjct: 31  IRCGPRDNRGPLLKGRILSTEAIQSIQSLKRAHRTGVS--LSLTLRPLRRLIKSDLISVL 90

Query: 93  KELLRQDRCALALEVFAVVRSEY-GADLGMYAEVAAALSRNGAAEEMDRLVCDLDDGESK 152
           +ELLRQD C LA+ V + +R+EY   DL +YA++  AL+RN   +E+DRL+ ++D  + +
Sbjct: 91  RELLRQDYCTLAVHVLSTLRTEYPPLDLVLYADIVNALTRNKEFDEIDRLIGEIDGIDQR 150

Query: 153 IQCDDKGLIKLIKAVIGGDRRESTVRIYRMMRRSGWGS-AIKADEYMVRVLSKGLRRLGE 212
              DDK L KLI+AV+G +RRES VR+Y +MR SGWGS + +ADEY+  VLSKGL RLGE
Sbjct: 151 --SDDKALAKLIRAVVGAERRESVVRVYTLMRESGWGSESWEADEYVAEVLSKGLLRLGE 210

Query: 213 VELADEIN 219
            +LA +++
Sbjct: 211 PDLASQVS 214

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BLAST of Lag0003018 vs. ExPASy Swiss-Prot
Match: Q9STF9 (Protein THYLAKOID ASSEMBLY 8-like, chloroplastic OS=Arabidopsis thaliana OX=3702 GN=THA8L PE=1 SV=1)

HSP 1 Score: 58.5 bits (140), Expect = 1.1e-07
Identity = 53/187 (28.34%), Postives = 97/187 (51.87%), Query Frame = 0


Query: 41  RGPLVKGRTL-STEAIQAIQSLKRAERSDPTKLQQVLSNTLSRLLKADLVAALKELLRQD 100
           RGPL +G+ L   EA+  I  LKR  + D  KL + +   + RLLK D++A + EL RQ+
Sbjct: 63  RGPLWRGKKLIGKEALFVILGLKRL-KEDDEKLDKFIKTHVFRLLKLDMLAVIGELERQE 122

Query: 101 RCALALEVFAVVRSE--YGADLGMYAEVAAALSRNGAAEEMDRLVCDLDDGESKIQCDDK 160
             ALA+++F V++ +  Y  D+ MY ++  +L+++   +E   L   +   +  +  D +
Sbjct: 123 ETALAIKMFEVIQKQEWYQPDVFMYKDLIVSLAKSKRMDEAMALWEKMK--KENLFPDSQ 182

Query: 161 GLIKLIKAVIGGDRRESTVRIYRMMRRSGWGSAIKADEYMVRVLSKGLRRLGEVELADEI 220
              ++I+  +        + +Y  M +    S    +E   RVL KGL  L    L +++
Sbjct: 183 TYTEVIRGFLRDGCPADAMNVYEDMLK----SPDPPEELPFRVLLKGL--LPHPLLRNKV 240

Query: 221 NREFQDL 225
            ++F++L
Sbjct: 243 KKDFEEL 240

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BLAST of Lag0003018 vs. ExPASy Swiss-Prot
Match: Q1PFH7 (Pentatricopeptide repeat-containing protein At1g62350 OS=Arabidopsis thaliana OX=3702 GN=At1g62350 PE=2 SV=1)

HSP 1 Score: 56.2 bits (134), Expect = 5.5e-07
Identity = 49/177 (27.68%), Postives = 91/177 (51.41%), Query Frame = 0


Query: 50  LSTEAIQAIQSLKRAERSDPTKLQQVLSNTLSRLLKADLVAALKELLRQDRCALALEVFA 109
           +S E + A + LKR + +   +L + + + +SRLLK+DLV+ L E  RQ++  L ++++ 
Sbjct: 1   MSKEGLIAAKELKRLQ-TQSVRLDRFIGSHVSRLLKSDLVSVLAEFQRQNQVFLCMKLYE 60

Query: 110 VVRSE--YGADLGMYAEVAAALSRNGAAEEMDRLVCDLDDGESKIQCDDKGLIKLIKAVI 169
           VVR E  Y  D+  Y ++   L+RN   +E  ++  DL   + ++  D      L++  +
Sbjct: 61  VVRREIWYRPDMFFYRDMLMMLARNKKVDETKKVWEDLK--KEEVLFDQHTFGDLVRGFL 120

Query: 170 GGDRRESTVRIYRMMRRSGWGSAIKADEYMVRVLSKGLRRLGEVELADEINREFQDL 225
             +     +R+Y  MR     S  +      RV+ KGL  +   EL +++  +F +L
Sbjct: 121 DNELPLEAMRLYGEMRE----SPDRPLSLPFRVILKGL--VPYPELREKVKDDFLEL 168

[Close]

BLAST of Lag0003018 vs. ExPASy TrEMBL
Match: A0A6J1JTE8 (protein THYLAKOID ASSEMBLY 8, chloroplastic OS=Cucurbita maxima OX=3661 GN=LOC111488709 PE=4 SV=1)

HSP 1 Score: 380.9 bits (977), Expect = 3.6e-102
Identity = 201/228 (88.16%), Postives = 212/228 (92.98%), Query Frame = 0


Query: 1   MAFSLHSTFLKSQFAIPIPISAATAAAAVSFPVRCGPRDNRGPLVKGRTLSTEAIQAIQS 60
           MAFSLHSTFLKSQ  IPIPIS ATA   VS PVRCGPRDNRGPLVKGRTLSTEAIQAIQS
Sbjct: 1   MAFSLHSTFLKSQIPIPIPIS-ATATVVVSLPVRCGPRDNRGPLVKGRTLSTEAIQAIQS 60

Query: 61  LKRAERSDPTKLQQVLSNTLSRLLKADLVAALKELLRQDRCALALEVFAVVRSEYGADLG 120
           LKRAE+SDPTKL+QVLS TLSRLLKADLVA LKELLRQDRCALALEVFAVVRSEYG DLG
Sbjct: 61  LKRAEKSDPTKLEQVLSTTLSRLLKADLVATLKELLRQDRCALALEVFAVVRSEYGVDLG 120

Query: 121 MYAEVAAALSRNGAAEEMDRLVCDLDDGESKIQCDDKGLIKLIKAVIGGDRRESTVRIYR 180
           MYAEVAAALSRNGA EE+DRLVCDL+D +  IQCDDKGLIKLIKAVIGGDRRESTVRIYR
Sbjct: 121 MYAEVAAALSRNGAMEEIDRLVCDLEDEDRVIQCDDKGLIKLIKAVIGGDRRESTVRIYR 180

Query: 181 MMRRSGWGSAIKADEYMVRVLSKGLRRLGEVELADEINREFQDLVGTF 229
           MM+RSGWGS IKAD+YMVRVLSKGLRRLGE+E+ADEIN +FQDLVG+F
Sbjct: 181 MMKRSGWGSTIKADDYMVRVLSKGLRRLGEMEMADEINMQFQDLVGSF 227

[Close]

BLAST of Lag0003018 vs. ExPASy TrEMBL
Match: A0A6J1GQS0 (protein THYLAKOID ASSEMBLY 8, chloroplastic OS=Cucurbita moschata OX=3662 GN=LOC111456620 PE=4 SV=1)

HSP 1 Score: 379.0 bits (972), Expect = 1.4e-101
Identity = 200/228 (87.72%), Postives = 212/228 (92.98%), Query Frame = 0


Query: 1   MAFSLHSTFLKSQFAIPIPISAATAAAAVSFPVRCGPRDNRGPLVKGRTLSTEAIQAIQS 60
           MAFSLHSTFLKSQ  IPIPISAA A   VS PVRCGPRDNRGPLVKGRTLSTEAIQAIQS
Sbjct: 1   MAFSLHSTFLKSQIPIPIPISAA-ATVVVSLPVRCGPRDNRGPLVKGRTLSTEAIQAIQS 60

Query: 61  LKRAERSDPTKLQQVLSNTLSRLLKADLVAALKELLRQDRCALALEVFAVVRSEYGADLG 120
           LKRAE+SDPTKL+QVLS TLSRLLKADLVA LKELLRQDRCALALEVFAVVRSEYGADLG
Sbjct: 61  LKRAEKSDPTKLEQVLSTTLSRLLKADLVATLKELLRQDRCALALEVFAVVRSEYGADLG 120

Query: 121 MYAEVAAALSRNGAAEEMDRLVCDLDDGESKIQCDDKGLIKLIKAVIGGDRRESTVRIYR 180
           MYAEVAAALSRNGA EE+DRLVCDL+D +  IQCDDKGLIKLIKAVIGGDRRESTVRIYR
Sbjct: 121 MYAEVAAALSRNGAMEEIDRLVCDLEDEDRVIQCDDKGLIKLIKAVIGGDRRESTVRIYR 180

Query: 181 MMRRSGWGSAIKADEYMVRVLSKGLRRLGEVELADEINREFQDLVGTF 229
           MM+RSGWGS IKAD+Y VRVLSKGLRRLGE+E+ADE+N +FQDLVG+F
Sbjct: 181 MMKRSGWGSTIKADDYTVRVLSKGLRRLGEMEMADEVNMQFQDLVGSF 227

[Close]

BLAST of Lag0003018 vs. ExPASy TrEMBL
Match: A0A1S3CGD3 (uncharacterized protein LOC103500597 OS=Cucumis melo OX=3656 GN=LOC103500597 PE=4 SV=1)

HSP 1 Score: 360.5 bits (924), Expect = 5.1e-96
Identity = 193/229 (84.28%), Postives = 209/229 (91.27%), Query Frame = 0


Query: 1   MAFSLHSTFLKSQFAIPIPISAATAAAAVSFPVRCGPRDNRGPLVKGRTLSTEAIQAIQS 60
           MA SLHSTFLKSQ +IPIP S ATAA AVSF VRCGPRDNRGPLVKGRTLS EAIQAIQS
Sbjct: 28  MASSLHSTFLKSQISIPIPTSTATAAVAVSFRVRCGPRDNRGPLVKGRTLSIEAIQAIQS 87

Query: 61  LKRAERSDPTKLQQVLSNTLSRLLKADLVAALKELLRQDRCALALEVFAVVRSEYGADLG 120
           LKRAERSDPTKLQQVLS TLSRLLKADLVA LKELLRQ+RCALALEVFAV+RSEY A+LG
Sbjct: 88  LKRAERSDPTKLQQVLSTTLSRLLKADLVATLKELLRQERCALALEVFAVIRSEYRAELG 147

Query: 121 MYAEVAAALSRNGAAEEMDRLVCDLD--DGESKIQCDDKGLIKLIKAVIGGDRRESTVRI 180
           +YAEVAAALSRNGAAEE+DRLVCDLD  DG  +   DDKGLIKLIKAVI G+RRESTVRI
Sbjct: 148 LYAEVAAALSRNGAAEEIDRLVCDLDGRDGVIEWGDDDKGLIKLIKAVISGNRRESTVRI 207

Query: 181 YRMMRRSGWGSAIKADEYMVRVLSKGLRRLGEVELADEINREFQDLVGT 228
           YRMMRR+GWGS IK D+YM++V+SKGLRR+GE+ELADEINREFQDLVG+
Sbjct: 208 YRMMRRNGWGSMIKGDDYMIKVMSKGLRRVGEIELADEINREFQDLVGS 256

[Close]

BLAST of Lag0003018 vs. ExPASy TrEMBL
Match: A0A0A0K6N6 (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_7G447050 PE=4 SV=1)

HSP 1 Score: 359.8 bits (922), Expect = 8.6e-96
Identity = 192/230 (83.48%), Postives = 209/230 (90.87%), Query Frame = 0


Query: 1   MAFSLHSTFLKSQFAIPIPISAATAAAAVSFPVRCGPRDNRGPLVKGRTLSTEAIQAIQS 60
           MA SLHSTFLKSQ +IPIP S AT+  AVSF VRCGPRDNRGPLVKGRTLS EAIQAIQS
Sbjct: 1   MASSLHSTFLKSQISIPIPASTATSPVAVSFRVRCGPRDNRGPLVKGRTLSIEAIQAIQS 60

Query: 61  LKRAERSDPTKLQQVLSNTLSRLLKADLVAALKELLRQDRCALALEVFAVVRSEYGADLG 120
           LKRAERSDPTKLQQVLS TLSRLLKADLVA LKELLRQ+RCALALEVFAV++SEY A+LG
Sbjct: 61  LKRAERSDPTKLQQVLSTTLSRLLKADLVATLKELLRQERCALALEVFAVIKSEYRAELG 120

Query: 121 MYAEVAAALSRNGAAEEMDRLVCDLDDGESKIQC--DDKGLIKLIKAVIGGDRRESTVRI 180
           +YAEVAAALSRNGAAEE+DRLV DLD G+  I+   DDKGLIKLIKAVI G+RRESTVRI
Sbjct: 121 LYAEVAAALSRNGAAEEIDRLVSDLDGGDGVIEWGDDDKGLIKLIKAVISGNRRESTVRI 180

Query: 181 YRMMRRSGWGSAIKADEYMVRVLSKGLRRLGEVELADEINREFQDLVGTF 229
           YRMMRR GWGS IKAD+YM++VLSKGLRRLGE+ELADEINREF+DLVG+F
Sbjct: 181 YRMMRRKGWGSMIKADDYMIKVLSKGLRRLGEIELADEINREFEDLVGSF 230

[Close]

BLAST of Lag0003018 vs. ExPASy TrEMBL
Match: A0A5A7V0E2 (Uncharacterized protein OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold347G002110 PE=4 SV=1)

HSP 1 Score: 356.7 bits (914), Expect = 7.3e-95
Identity = 192/229 (83.84%), Postives = 208/229 (90.83%), Query Frame = 0


Query: 1   MAFSLHSTFLKSQFAIPIPISAATAAAAVSFPVRCGPRDNRGPLVKGRTLSTEAIQAIQS 60
           MA SL STFLKSQ +IPIP S ATAA AVSF VRCGPRDNRGPLVKGRTLS EAIQAIQS
Sbjct: 1   MASSLRSTFLKSQISIPIPTSTATAAVAVSFRVRCGPRDNRGPLVKGRTLSIEAIQAIQS 60

Query: 61  LKRAERSDPTKLQQVLSNTLSRLLKADLVAALKELLRQDRCALALEVFAVVRSEYGADLG 120
           LKRAERSDPTKLQQVLS TLSRLLKADLVA LKELLRQ+RCALALEVFAV+RSEY A+LG
Sbjct: 61  LKRAERSDPTKLQQVLSTTLSRLLKADLVATLKELLRQERCALALEVFAVIRSEYRAELG 120

Query: 121 MYAEVAAALSRNGAAEEMDRLVCDLD--DGESKIQCDDKGLIKLIKAVIGGDRRESTVRI 180
           +YAEVAAALSRNGAAEE+DRLVCDLD  DG  +   DDKGLIKLIKAVI G+RRESTVRI
Sbjct: 121 LYAEVAAALSRNGAAEEIDRLVCDLDGRDGVIEWGDDDKGLIKLIKAVISGNRRESTVRI 180

Query: 181 YRMMRRSGWGSAIKADEYMVRVLSKGLRRLGEVELADEINREFQDLVGT 228
           YRMMRR+GWGS IK D+YM++V+SKGLRR+GE+ELADEINREFQDLVG+
Sbjct: 181 YRMMRRNGWGSMIKGDDYMIKVMSKGLRRVGELELADEINREFQDLVGS 229

[Close]

BLAST of Lag0003018 vs. TAIR 10
Match: AT3G27750.1 (FUNCTIONS IN: molecular_function unknown; INVOLVED IN: biological_process unknown; LOCATED IN: chloroplast; EXPRESSED IN: 16 plant structures; EXPRESSED DURING: 12 growth stages; BEST Arabidopsis thaliana protein match is: Vacuolar sorting protein 9 (VPS9) domain (TAIR:AT5G09320.1); Has 106 Blast hits to 106 proteins in 16 species: Archae - 0; Bacteria - 0; Metazoa - 0; Fungi - 4; Plants - 102; Viruses - 0; Other Eukaryotes - 0 (source: NCBI BLink). )

HSP 1 Score: 186.4 bits (472), Expect = 2.5e-47
Identity = 102/188 (54.26%), Postives = 140/188 (74.47%), Query Frame = 0


Query: 33  VRCGPRDNRGPLVKGRTLSTEAIQAIQSLKRAERSDPTKLQQVLSNTLSRLLKADLVAAL 92
           +RCGPRDNRGPL+KGR LSTEAIQ+IQSLKRA R+  +    +    L RL+K+DL++ L
Sbjct: 31  IRCGPRDNRGPLLKGRILSTEAIQSIQSLKRAHRTGVS--LSLTLRPLRRLIKSDLISVL 90

Query: 93  KELLRQDRCALALEVFAVVRSEY-GADLGMYAEVAAALSRNGAAEEMDRLVCDLDDGESK 152
           +ELLRQD C LA+ V + +R+EY   DL +YA++  AL+RN   +E+DRL+ ++D  + +
Sbjct: 91  RELLRQDYCTLAVHVLSTLRTEYPPLDLVLYADIVNALTRNKEFDEIDRLIGEIDGIDQR 150

Query: 153 IQCDDKGLIKLIKAVIGGDRRESTVRIYRMMRRSGWGS-AIKADEYMVRVLSKGLRRLGE 212
              DDK L KLI+AV+G +RRES VR+Y +MR SGWGS + +ADEY+  VLSKGL RLGE
Sbjct: 151 --SDDKALAKLIRAVVGAERRESVVRVYTLMRESGWGSESWEADEYVAEVLSKGLLRLGE 210

Query: 213 VELADEIN 219
            +LA +++
Sbjct: 211 PDLASQVS 214

[Close]

BLAST of Lag0003018 vs. TAIR 10
Match: AT5G09320.1 (Vacuolar sorting protein 9 (VPS9) domain )

HSP 1 Score: 82.8 bits (203), Expect = 3.9e-16
Identity = 64/203 (31.53%), Postives = 101/203 (49.75%), Query Frame = 0


Query: 40  NRGPLVKGRTLSTEAIQAIQSLKRAE--------------RSDPTKLQQVLSNTLSRLLK 99
           NR PL +GR LS EAIQA+Q+LKRA                S    L +V+ +   RLLK
Sbjct: 495 NRKPLQRGRMLSIEAIQAVQALKRANPLLPPPPVPSTSTTSSSSALLDRVIISKFRRLLK 554

Query: 100 ADLVAALKELLRQDRCALALEVFAVVRSE--YGADLGMYAEVAAALSRNGAAEEMDRLVC 159
            D+VA L+ELLRQ+ C+LAL+VF  +R E  Y   + MY ++   ++ N   EE++ L  
Sbjct: 555 FDMVAVLRELLRQNECSLALKVFEEIRKEYWYKPQVRMYTDMITVMADNSLMEEVNYLYS 614

Query: 160 DLDDGESKIQCDDKGLIKLIKAVIGGDRRESTVRIYRMMRRSGWGSAIKADEYMVRVLSK 219
            +   E  +  + +    L+  ++     +  +  Y  M+  G+    + D    RVL  
Sbjct: 615 AM-KSEKGLMAEIEWFNTLLTILLNHKLFDLVMDCYAFMQSIGY----EPDRASFRVLVL 674

Query: 220 GLRRLGEVELADEINREFQDLVG 227
           GL   GE+ L+  + ++  +  G
Sbjct: 675 GLESNGEMGLSAIVRQDAHEYYG 692

[Close]

BLAST of Lag0003018 vs. TAIR 10
Match: AT3G46870.1 (Pentatricopeptide repeat (PPR) superfamily protein )

HSP 1 Score: 58.5 bits (140), Expect = 7.9e-09
Identity = 53/187 (28.34%), Postives = 97/187 (51.87%), Query Frame = 0


Query: 41  RGPLVKGRTL-STEAIQAIQSLKRAERSDPTKLQQVLSNTLSRLLKADLVAALKELLRQD 100
           RGPL +G+ L   EA+  I  LKR  + D  KL + +   + RLLK D++A + EL RQ+
Sbjct: 63  RGPLWRGKKLIGKEALFVILGLKRL-KEDDEKLDKFIKTHVFRLLKLDMLAVIGELERQE 122

Query: 101 RCALALEVFAVVRSE--YGADLGMYAEVAAALSRNGAAEEMDRLVCDLDDGESKIQCDDK 160
             ALA+++F V++ +  Y  D+ MY ++  +L+++   +E   L   +   +  +  D +
Sbjct: 123 ETALAIKMFEVIQKQEWYQPDVFMYKDLIVSLAKSKRMDEAMALWEKMK--KENLFPDSQ 182

Query: 161 GLIKLIKAVIGGDRRESTVRIYRMMRRSGWGSAIKADEYMVRVLSKGLRRLGEVELADEI 220
              ++I+  +        + +Y  M +    S    +E   RVL KGL  L    L +++
Sbjct: 183 TYTEVIRGFLRDGCPADAMNVYEDMLK----SPDPPEELPFRVLLKGL--LPHPLLRNKV 240

Query: 221 NREFQDL 225
            ++F++L
Sbjct: 243 KKDFEEL 240

[Close]

BLAST of Lag0003018 vs. TAIR 10
Match: AT1G62350.1 (Pentatricopeptide repeat (PPR) superfamily protein )

HSP 1 Score: 56.2 bits (134), Expect = 3.9e-08
Identity = 49/177 (27.68%), Postives = 91/177 (51.41%), Query Frame = 0


Query: 50  LSTEAIQAIQSLKRAERSDPTKLQQVLSNTLSRLLKADLVAALKELLRQDRCALALEVFA 109
           +S E + A + LKR + +   +L + + + +SRLLK+DLV+ L E  RQ++  L ++++ 
Sbjct: 1   MSKEGLIAAKELKRLQ-TQSVRLDRFIGSHVSRLLKSDLVSVLAEFQRQNQVFLCMKLYE 60

Query: 110 VVRSE--YGADLGMYAEVAAALSRNGAAEEMDRLVCDLDDGESKIQCDDKGLIKLIKAVI 169
           VVR E  Y  D+  Y ++   L+RN   +E  ++  DL   + ++  D      L++  +
Sbjct: 61  VVRREIWYRPDMFFYRDMLMMLARNKKVDETKKVWEDLK--KEEVLFDQHTFGDLVRGFL 120

Query: 170 GGDRRESTVRIYRMMRRSGWGSAIKADEYMVRVLSKGLRRLGEVELADEINREFQDL 225
             +     +R+Y  MR     S  +      RV+ KGL  +   EL +++  +F +L
Sbjct: 121 DNELPLEAMRLYGEMRE----SPDRPLSLPFRVILKGL--VPYPELREKVKDDFLEL 168

The following BLAST results are available for this feature:

BLAST of Lag0003018 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_023522652.1	3.3e-102	88.16	protein THYLAKOID ASSEMBLY 8, chloroplastic-like [Cucurbita pepo subsp. pepo] >X...	[more]
XP_022992386.1	7.5e-102	88.16	protein THYLAKOID ASSEMBLY 8, chloroplastic [Cucurbita maxima]	[more]
KAG7014066.1	1.3e-101	87.72	Pentatricopeptide repeat-containing protein, partial [Cucurbita argyrosperma sub...	[more]
XP_022954347.1	2.8e-101	87.72	protein THYLAKOID ASSEMBLY 8, chloroplastic [Cucurbita moschata]	[more]
XP_038898717.1	1.1e-100	88.94	protein THYLAKOID ASSEMBLY 8, chloroplastic [Benincasa hispida]	[more]

BLAST of Lag0003018 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
Q9LVW6	3.5e-46	54.26	Protein THYLAKOID ASSEMBLY 8, chloroplastic OS=Arabidopsis thaliana OX=3702 GN=T...	[more]
Q9STF9	1.1e-07	28.34	Protein THYLAKOID ASSEMBLY 8-like, chloroplastic OS=Arabidopsis thaliana OX=3702...	[more]
Q1PFH7	5.5e-07	27.68	Pentatricopeptide repeat-containing protein At1g62350 OS=Arabidopsis thaliana OX...	[more]

BLAST of Lag0003018 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A6J1JTE8	3.6e-102	88.16	protein THYLAKOID ASSEMBLY 8, chloroplastic OS=Cucurbita maxima OX=3661 GN=LOC11...	[more]
A0A6J1GQS0	1.4e-101	87.72	protein THYLAKOID ASSEMBLY 8, chloroplastic OS=Cucurbita moschata OX=3662 GN=LOC...	[more]
A0A1S3CGD3	5.1e-96	84.28	uncharacterized protein LOC103500597 OS=Cucumis melo OX=3656 GN=LOC103500597 PE=...	[more]
A0A0A0K6N6	8.6e-96	83.48	Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_7G447050 PE=4 SV=1	[more]
A0A5A7V0E2	7.3e-95	83.84	Uncharacterized protein OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold...	[more]

BLAST of Lag0003018 vs. TAIR 10


Match Name	E-value	Identity	Description
AT3G27750.1	2.5e-47	54.26	FUNCTIONS IN: molecular_function unknown; INVOLVED IN: biological_process unknow...	[more]
AT5G09320.1	3.9e-16	31.53	Vacuolar sorting protein 9 (VPS9) domain	[more]
AT3G46870.1	7.9e-09	28.34	Pentatricopeptide repeat (PPR) superfamily protein	[more]
AT1G62350.1	3.9e-08	27.68	Pentatricopeptide repeat (PPR) superfamily protein	[more]

InterPro

Analysis Name: InterPro Annotations of Sponge gourd (AG-4) v1
Date Performed: 2022-08-01

Position :

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IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR011990	Tetratricopeptide-like helical domain superfamily	GENE3D	1.25.40.10	Tetratricopeptide repeat domain	coord: 49..224 e-value: 4.1E-32 score: 113.2
None	No IPR available	PANTHER	PTHR47594:SF3	PROTEIN THYLAKOID ASSEMBLY 8, CHLOROPLASTIC	coord: 15..226
IPR044190	Protein THYLAKOID ASSEMBLY 8-like	PANTHER	PTHR47594	PPR CONTAINING PLANT-LIKE PROTEIN	coord: 15..226

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
Lag0003018.1	Lag0003018.1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0009658	chloroplast organization
biological_process	GO:0000373	Group II intron splicing
molecular_function	GO:0005515	protein binding
molecular_function	GO:0003723	RNA binding